Search

Your search keyword '"BAJPAI, VIVEK K."' showing total 672 results
Start Over Author "BAJPAI, VIVEK K."
672 results on '"BAJPAI, VIVEK K."'

2. Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos

Author

Roodgar, Morteza, Suchy, Fabian P, Nguyen, Lan H, Bajpai, Vivek K, Sinha, Rahul, Vilches-Moure, Jose G, Van Bortle, Kevin, Bhadury, Joydeep, Metwally, Ahmed, Jiang, Lihua, Jian, Ruiqi, Chiang, Rosaria, Oikonomopoulos, Angelos, Wu, Joseph C, Weissman, Irving L, Mankowski, Joseph L, Holmes, Susan, Loh, Kyle M, Nakauchi, Hiromitsu, VandeVoort, Catherine A, and Snyder, Michael P

Subjects
Biological Sciences, Stem Cell Research, Stem Cell Research - Induced Pluripotent Stem Cell, Biotechnology, Stem Cell Research - Nonembryonic - Non-Human, Animals, Induced Pluripotent Stem Cells, Macaca mulatta, Macaca nemestrina, Pan troglodytes, Proteomics, CP: Developmental biology, Nonhuman primates, blastocyst, BCL2, chimpanzee, cross-species embryos, iPSCs, interspecies chimera, pig-tailed macaque, Biochemistry and Cell Biology, Medical Physiology, Biological sciences
Abstract

As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research.

Published
2022

3. Human–chimpanzee fused cells reveal cis-regulatory divergence underlying skeletal evolution

Author

Gokhman, David, Agoglia, Rachel M, Kinnebrew, Maia, Gordon, Wei, Sun, Danqiong, Bajpai, Vivek K, Naqvi, Sahin, Chen, Coral, Chan, Anthony, Chen, Chider, Petrov, Dmitri A, Ahituv, Nadav, Zhang, Honghao, Mishina, Yuji, Wysocka, Joanna, Rohatgi, Rajat, and Fraser, Hunter B

Subjects
Biochemistry and Cell Biology, Biological Sciences, Dental/Oral and Craniofacial Disease, Stem Cell Research, Genetics, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Animals, Biological Evolution, Cell Differentiation, Chimera, Ellis-Van Creveld Syndrome, Female, Gene Expression, Genotype, Humans, Induced Pluripotent Stem Cells, Intercellular Signaling Peptides and Proteins, Male, Mice, Mice, Knockout, Neural Crest, Pan troglodytes, Phenotype, Signal Transduction, Skull, Species Specificity, Tetraploidy, Medical and Health Sciences, Developmental Biology, Agricultural biotechnology, Bioinformatics and computational biology
Abstract

Gene regulatory divergence is thought to play a central role in determining human-specific traits. However, our ability to link divergent regulation to divergent phenotypes is limited. Here, we utilized human-chimpanzee hybrid induced pluripotent stem cells to study gene expression separating these species. The tetraploid hybrid cells allowed us to separate cis- from trans-regulatory effects, and to control for nongenetic confounding factors. We differentiated these cells into cranial neural crest cells, the primary cell type giving rise to the face. We discovered evidence of lineage-specific selection on the hedgehog signaling pathway, including a human-specific sixfold down-regulation of EVC2 (LIMBIN), a key hedgehog gene. Inducing a similar down-regulation of EVC2 substantially reduced hedgehog signaling output. Mice and humans lacking functional EVC2 show striking phenotypic parallels to human-chimpanzee craniofacial differences, suggesting that the regulatory divergence of hedgehog signaling may have contributed to the unique craniofacial morphology of humans.

Published
2021

18. Revisiting the plant growth-promoting rhizobacteria: lessons from the past and objectives for the future

Author

Aeron, Abhinav, Khare, Ekta, Jha, Chaitanya Kumar, Meena, Vijay Singh, Aziz, Shadia Mohammed Abdel, Islam, Mohammed Tofazzal, Kim, Kangmin, Meena, Sunita Kumari, Pattanayak, Arunava, Rajashekara, Hosahatti, Dubey, Ramesh Chandra, Maurya, Bihari Ram, Maheshwari, Dinesh Kumar, Saraf, Meenu, Choudhary, Mahipal, Verma, Rajhans, Meena, H. N., Subbanna, A. R. N. S., Parihar, Manoj, Shukla, Shruti, Muthusamy, Govarthanan, Bana, Ram Swaroop, Bajpai, Vivek K., Han, Young-Kyu, Rahman, Mahfuzur, Kumar, Dileep, Singh, Norang Pal, and Meena, Rajesh Kumar

Published
2020
Full Text
View/download PDF

31. Mycotoxins in food and feed: toxicity, preventive challenges, and advanced detection techniques for associated diseases.

Author

Dey, Debasish Kumar, Kang, Ji In, Bajpai, Vivek K., Kim, Kwanwoo, Lee, Hoomin, Sonwal, Sonam, Simal-Gandara, Jesus, Xiao, Jianbo, Ali, Sajad, Huh, Yun Suk, Han, Yong-Kyu, and Shukla, Shruti

Subjects
DISEASE complications, FEED contamination, FUNGAL metabolites, FOOD contamination, METABOLITES, MYCOTOXINS
Abstract

Mycotoxins are produced primarily as secondary fungal metabolites. Mycotoxins are toxic in nature and naturally produced by various species of fungi, which usually contaminate food and feed ingredients. The growth of these harmful fungi depends on several environmental factors, such as pH, humidity, and temperature; therefore, the mycotoxin distribution also varies among global geographical areas. Various rules and regulations regarding mycotoxins are imposed by the government bodies of each country, which are responsible for addressing global food and health security concerns. Despite this legislation, the incidence of mycotoxin contamination is continuously increasing. In this review, we discuss the geographical regulatory guidelines and recommendations that are implemented around the world to control mycotoxin contamination of food and feed products. Researchers and inventors from various parts of the world have reported several innovations for controlling mycotoxin-associated health consequences. Unfortunately, most of these techniques are restricted to laboratory scales and cannot reach users. Consequently, to date, no single device has been commercialized that can detect all mycotoxins that are naturally available in the environment. Therefore, in this study, we describe severe health hazards that are associated with mycotoxin exposure, their molecular signaling pathways and processes of toxicity, and their genotoxic and cytotoxic effects toward humans and animals. We also discuss recent developments in the construction of a sensitive and specific device that effectively implements mycotoxin identification and detection methods. In addition, our study comprehensively examines the recent advancements in the field for mitigating the health consequences and links them with the molecular and signaling pathways that are activated upon mycotoxin exposure. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

38. Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L.

Author

Kumar Pankaj, Dubey Ramesh Chandra, Maheshwari Dinesh Kumar, Park Yong-Ha, and Bajpai Vivek K.

Subjects
Pseudomonas sp. PPR8, Phaseolus vulgaris, plant growth promotion, biocontrol, Biology (General), QH301-705.5
Abstract

In vitro screening of plant growth-promoting (PGP) traits was carried out using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All the isolates were fast growers, positive for catalase, oxidase and urease activities, and utilized lactose and some amino acids. All the isolates were indole acetic acid (IAA) positive, however PPR8 solubilized potassium and zinc along with various other types of inorganic (tricalcium, dicalcium and zinc phosphate) and organic (calcium phytate) phosphates, as well as producing siderophore and ACC deaminase. PPR8 also produced cyanogens, extracellular chitinase, β-1,3-glucanase, β-1,4-glucanase and oxalate oxidase. Based on the PGP traits of all isolates, PPR8 was found to be the most potent plant growth-promoting rhizobacteria (PGPR). Further, PPR8 was identified as Pseudomonas sp. PPR8, based on 16S rRNA gene sequencing analysis. Moreover, the PGP activities of PPR8 confirmed it to be a potent biocontrol agent, inhibiting the growth of various plant pathogenic fungi. This study reveals the potential of Pseudomonas sp. PPR8 to be used as a good bioinoculant for growth promotion of common bean and for the protection of important legume crops from various deleterious phytopathogens.

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results