Search

This study aims to provide improved knowledge and evidence on current (1986–2015) climate variation based on six rainfall indices over five West African countries (Senegal, Niger, Burkina Faso, Ivory Coast, and Benin) using the Climate Hazards Group InfraRed Precipitation with Station (CHIRPS) dataset. On average, precipitation has increased over the central Sahel and the western Sahel. This increase is associated with increase in the number of rainy days, longer wet spells and shorter dry spells. Over the Guinea Coast, the slight increase in precipitation is associated with an increase in the intensity of rainfall with a shorter duration of wet spells. However, these mean changes in precipitation are not all statistically significant and uniform within a country. While previous studies are focused on regional and sub-regional scales, this study contributes to deliver a climate information at a country level that is more relevant for decision making and for policy makers, and to document climate-related risks within a country to feed impact studies in key sectors of the development, such as agriculture and water resources.

This study uses the high resolution outputs of the recent CORDEX-AFRICA climate projections to investigate the future changes in different aspects of the hydrological cycle over West Africa. Over the twenty-first century, temperatures in West Africa are expected to increase at a faster rate (+ 0.5 °C per decade) than the global average (+ 0.3 °C per decade), and mean precipitation is expected to increase over the Guinea Coast (+ 0.03 mm/day per decade) but decrease over the Sahel (- 0.005 mm/day per decade). In addition, precipitation is expected to become more intense (+ 0.2 mm/day per decade) and less frequent (- 1.5 days per decade) over the entire West Africa as a results of increasing regional temperature (precipitation intensity increases on average by + 0.35 mm/day per °C and precipitation frequency decreases on average by - 2.2 days per °C). Over the Sahel, the average length of dry spells is also expected to increase with temperature (+ 4 % days per °C), which increases the likelihood for droughts with warming in this sub-region. Hence, the hydrological cycle is expected to increase throughout the twenty-first century over the entire West Africa, on average by + 11 % per °C over the Sahel as a result of increasing precipitation intensity and lengthening of dry spells, and on average by + 3 % per °C over the Guinea Coast as a result of increasing precipitation intensity only. [ABSTRACT FROM AUTHOR]

The synthesis of cellulose microfibrils requires the presence of a membrane-bound endo-1,4-β-D-glucanase, KORRIGAN1 (KOR1). Although the exact biochemical role of KOR1 in cellulose synthesis is unknown, we used the protein as a marker to explore the potential involvement of subcellular transport processes in cellulose synthesis. Using immunofluorescence and a green fluorescent protein (GFP)-KOR1 fusion that complemented the phenotype conferred by the kor1-1 mutant, we investigated the distribution of KOR1 in epidermal cells in the root meristem. KOR1 was localized in intracellular compartments corresponding to a heterogeneous population of organelles, which comprised the Golgi apparatus, FM4-64-labeled compartments referred to as early endosomes, and, in the case of GFP-KOR1, the tonoplast. Inhibition of cellulose synthesis by isoxaben promoted a net redistribution of GFP-KOR1 toward a homogeneous population of compartments, distinct from early endosomes, which were concentrated close to the plasma membrane facing the root surface. A redistribution of GFP-KOR1 away from early endosomes was also observed in the same cells at later stages of cell elongation. A subpopulation of GFP-KOR1-containing compartments followed trajectories along the plasma membrane, and this motility required intact microtubules. These observations demonstrate that the deposition of cellulose, like chitin synthesis in yeast, involves the regulated intracellular cycling of at least one enzyme required for its synthesis. [ABSTRACT FROM AUTHOR]

The synthesis of cellulose microfibrils requires the presence of a membrane-bound endo-1,4-β-D-glucanase, KORRIGAN1 (KOR1). Although the exact biochemical role of KOR1 in cellulose synthesis is unknown, we used the protein as a marker to explore the potential involvement of subcellular transport processes in cellulose synthesis. Using immunofluorescence and a green fluorescent protein (GFP)-KOR1 fusion that complemented the phenotype conferred by the kor1-1 mutant, we investigated the distribution of KOR1 in epidermal cells in the root meristem. KOR1 was localized in intracellular compartments corresponding to a heterogeneous population of organelles, which comprised the Golgi apparatus, FM4-64-labeled compartments referred to as early endosomes, and, in the case of GFP-KOR1, the tonoplast. Inhibition of cellulose synthesis by isoxaben promoted a net redistribution of GFP-KOR1 toward a homogeneous population of compartments, distinct from early endosomes, which were concentrated close to the plasma membrane facing the root surface. A redistribution of GFP-KOR1 away from early endosomes was also observed in the same cells at later stages of cell elongation. A subpopulation of GFP-KOR1-containing compartments followed trajectories along the plasma membrane, and this motility required intact microtubules. These observations demonstrate that the deposition of cellulose, like chitin synthesis in yeast, involves the regulated intracellular cycling of at least one enzyme required for its synthesis. [ABSTRACT FROM AUTHOR]

Gene regulation networks control differentiation and function of hundreds of cell types. Dysfunctions of transcription factors, which are key elements in the regulation pathways, are involved in numerous pathologies. The recent development of genomics technologies allows the study of gene regulation mechanisms and help us understand their impact on the cells. Bioinformatic tools are needed to fully exploit data obtained by genomics approaches. Thus, bioinformatics play an essential role in the characterisation of the transcription factors binding sites and their target genes. In this review we will introduce the main breakthroughs in bioinformatics area for the comprehension of regulation mechanisms. We will insist on i) the approaches "with a priori" for the genome annotation based on known transcription factors binding sites, ii) the approaches "without a priori" for the discovery of new binding sites and iii) the functional annotation of the target genes of those transcription factors. Finally, we will present recent examples of the fruitful use of in silico studies for the comprehension of regulation mechanisms and of the consequences of their dysfunction.