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1. CD146 at the Interface between Oxidative Stress and the Wnt Signaling Pathway in Systemic Sclerosis

Author

Heim, Xavier, Bermudez, Julien, Joshkon, Ahmad, Kaspi, Elise, Bachelier, Richard, Nollet, Marie, Vélier, Mélanie, Dou, Laetitia, Brodovitch, Alexandre, Foucault-Bertaud, Alexandrine, Leroyer, Aurelie S., Benyamine, Audrey, Daumas, Aurélie, Granel, Brigitte, Sabatier, Florence, Dignat-George, Françoise, Blot-Chabaud, Marcel, and Bardin, Nathalie

Published
2022
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2. Caspase-1 activation, IL-1/IL-6 signature and IFNγ-induced chemokines in lungs of COVID-19 patients.

Author

Cambon, Audrey, Guervilly, Christophe, Delteil, Clémence, Potere, Nicola, Bachelier, Richard, Tellier, Edwige, Abdili, Evelyne, Leprince, Marine, Giani, Marco, Polidoro, Ildo, Albanese, Valentina, Ferrante, Paolo, Coffin, Laurence, Schiffrin, Michael, Arnaud, Laurent, Lacroix, Romaric, Roque, Sandrine, Forel, Jean-Marie, Hraiech, Sami, and Daniel, Laurent

Subjects
ADULT respiratory distress syndrome, COVID-19, CASPASES, IMMUNE response, LUNGS
Abstract

Rationale: COVID-19-associated acute-respiratory distress syndrome (C-ARDS) results from a direct viral injury associated with host excessive innate immune response mainly affecting the lungs. However, cytokine profile in the lung compartment of C-ARDS patients has not been widely studied, nor compared to non-COVID related ARDS (NC-ARDS). Objectives: To evaluate caspase-1 activation, IL-1 signature, and other inflammatory cytokine pathways associated with tissue damage using post-mortem lung tissues, bronchoalveolar lavage fluids (BALF), and serum across the spectrum of COVID-19 severity. Methods: Histological features were described and activated-caspase-1 labeling was performed in 40 post-mortem biopsies. Inflammatory cytokines were quantified in BALF and serum from 19 steroid-treated-C-ARDSand compared to 19 NC-ARDS. Cytokine concentrations were also measured in serum from 128 COVID-19 patients at different severity stages. Measurements and main results: Typical "diffuse alveolar damage" in lung biopsies were associated with activated caspase-1 expression and vascular lesions. Soluble Caspase-1p20, IL-1β, IL-1Ra, IL-6 and at lower level IFNγ and CXCL-10, were highly elevated in BALF from steroid-treated-C-ARDS as well as in NC-ARDS. IL-1β appeared concentrated in BALF, whereas circulating IL-6 and IL-1Ra concentrations were comparable to those in BALF and correlated with severity. TNFα, TNFR1 and CXCL8 however, were significantly higher in NC-ARDS compared to C-ARDS, treated by steroid. Conclusions: In the lungs of C-ARDS, both caspase-1 activation with a predominant IL-1β/IL-6 signature and IFNγ -associated chemokines are elevated despite steroid treatment. These pathways may be specifically targeted in ARDS to improve response to treatment and to limit alveolar and vascular lung damage. [ABSTRACT FROM AUTHOR]

Published
2025
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3. Soluble CD146 is increased in preeclampsia and interacts with galectin-1 to regulate trophoblast migration through VEGFR2 receptor

Author

Bouvier, Sylvie, Traboulsi, Waël, Blois, Sandra M., Demattei, Christophe, Joshkon, Ahmad, Mousty, Eve, Nollet, Marie, Paulmyer-Lacroix, Odile, Foucault-Bertaud, Alexandrine, Fortier, Mathieu, Leroyer, Aurélie S., Bachelier, Richard, Letouzey, Vincent, Alfaidy, Nadia, Dignat-George, Françoise, Blot-Chabaud, Marcel, Gris, Jean-Christophe, and Bardin, Nathalie

Published
2022
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7. Macrophage IL-1β-positive microvesicles exhibit thrombo-inflammatory properties and are detectable in patients with active juvenile idiopathic arthritis

Author

Cambon, Audrey, primary, Rebelle, Charlotte, additional, Bachelier, Richard, additional, Arnaud, Laurent, additional, Robert, Stéphane, additional, Lagarde, Marie, additional, Muller, Romain, additional, Tellier, Edwige, additional, Kara, Yéter, additional, Leroyer, Aurélie, additional, Farnarier, Catherine, additional, Vallier, Loris, additional, Chareyre, Corinne, additional, Retornaz, Karine, additional, Jurquet, Anne-Laure, additional, Tran, Tu-Anh, additional, Lacroix, Romaric, additional, Dignat-George, Françoise, additional, and Kaplanski, Gilles, additional

Published
2023
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9. Macrophage IL-1b-positive microvesicles exhibit thromboinflammatory properties and are detectable in patients with active juvenile idiopathic arthritis.

Author

Cambon, Audrey, Rebelle, Charlotte, Bachelier, Richard, Arnaud, Laurent, Robert, Stéphane, Lagarde, Marie, Muller, Romain, Tellier, Edwige, Kara, Yéter, Leroyer, Aurélie, Farnarier, Catherine, Vallier, Loris, Chareyre, Corinne, Retornaz, Karine, Jurquet, Anne-Laure, Tran, Tu-Anh, Lacroix, Romaric, Dignat-George, Françoise, and Kaplanski, Gilles

Subjects
JUVENILE idiopathic arthritis, EXTRACELLULAR vesicles, INTERLEUKIN-1, MACROPHAGES, NLRP3 protein
Abstract

Objective: IL-1b is a leaderless cytokine with poorly known secretory mechanisms that is barely detectable in serum of patients, including those with an IL-1b-mediated disease such as systemic juvenile idiopathic arthritis (sJIA). Leukocyte microvesicles (MVs) may be a mechanism of IL-1b secretion. The first objective of our study was to characterize IL-1b-positive MVs obtained from macrophage cell culture supernatants and to investigate their biological functions in vitro and in vivo. The second objective was to detect circulating IL-1b-positive MVs in JIA patients. Methods: MVs were purified by serial centrifugations from PBMCs, or THP-1 differentiated into macrophages, then stimulated with LPS ± ATP. MV content was analyzed for the presence of IL-1b, NLRP3 inflammasome, caspase-1, P2X7 receptor, and tissue factor (TF) using ELISA, Western blot, or flow cytometry. MV biological properties were studied in vitro by measuring VCAM-1, ICAM-1, and Eselectin expression after HUVEC co-culture and factor-Xa generation test was realized. In vivo, MVs’ ability to recruit leukocytes in a murine model of peritonitis was evaluated. Plasmatic IL-1b-positive MVs were studied ex vivo in 10 active JIA patients using flow cytometry. Results: THP-1-derived macrophages stimulated with LPS and ATP released MVs, which contained NLRP3, caspase-1, and the 33-kDa precursor and 17-kDa mature forms of IL-1b and bioactive TF. IL-1b-positive MVs expressed P2X7 receptor and released soluble IL-1b in response to ATP stimulation in vitro. In mice, MVs induced a leukocyte peritoneal infiltrate, which was reduced by treatment with the IL-1 receptor antagonist. Finally, IL-1b-positive MVs were detectable in plasma from 10 active JIA patients. Conclusion: MVs shed from activated macrophages contain IL-1b, NLRP3 inflammasome components, and TF, and constitute thrombo-inflammatory vectors that can be detected in the plasma from active JIA patients. [ABSTRACT FROM AUTHOR]

Published
2023
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10. supplementary methods from miRNA-30 Family Members Inhibit Breast Cancer Invasion, Osteomimicry, and Bone Destruction by Directly Targeting Multiple Bone Metastasis–Associated Genes

Author

Croset, Martine, primary, Pantano, Francesco, primary, Kan, Casina W.S., primary, Bonnelye, Edith, primary, Descotes, Françoise, primary, Alix-Panabières, Catherine, primary, Lecellier, Charles-Henri, primary, Bachelier, Richard, primary, Allioli, Nathalie, primary, Hong, Saw-See, primary, Bartkowiak, Kai, primary, Pantel, Klaus, primary, and Clézardin, Philippe, primary

Published
2023
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11. Figures S1 to S11 from miRNA-30 Family Members Inhibit Breast Cancer Invasion, Osteomimicry, and Bone Destruction by Directly Targeting Multiple Bone Metastasis–Associated Genes

Author

Croset, Martine, primary, Pantano, Francesco, primary, Kan, Casina W.S., primary, Bonnelye, Edith, primary, Descotes, Françoise, primary, Alix-Panabières, Catherine, primary, Lecellier, Charles-Henri, primary, Bachelier, Richard, primary, Allioli, Nathalie, primary, Hong, Saw-See, primary, Bartkowiak, Kai, primary, Pantel, Klaus, primary, and Clézardin, Philippe, primary

Published
2023
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12. Data from miRNA-30 Family Members Inhibit Breast Cancer Invasion, Osteomimicry, and Bone Destruction by Directly Targeting Multiple Bone Metastasis–Associated Genes

Author

Croset, Martine, primary, Pantano, Francesco, primary, Kan, Casina W.S., primary, Bonnelye, Edith, primary, Descotes, Françoise, primary, Alix-Panabières, Catherine, primary, Lecellier, Charles-Henri, primary, Bachelier, Richard, primary, Allioli, Nathalie, primary, Hong, Saw-See, primary, Bartkowiak, Kai, primary, Pantel, Klaus, primary, and Clézardin, Philippe, primary

Published
2023
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13. Supplementary Figure Legends from miRNA-30 Family Members Inhibit Breast Cancer Invasion, Osteomimicry, and Bone Destruction by Directly Targeting Multiple Bone Metastasis–Associated Genes

Author

Croset, Martine, primary, Pantano, Francesco, primary, Kan, Casina W.S., primary, Bonnelye, Edith, primary, Descotes, Françoise, primary, Alix-Panabières, Catherine, primary, Lecellier, Charles-Henri, primary, Bachelier, Richard, primary, Allioli, Nathalie, primary, Hong, Saw-See, primary, Bartkowiak, Kai, primary, Pantel, Klaus, primary, and Clézardin, Philippe, primary

Published
2023
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15. Supplementary Figure 1 from Bone Morphogenetic Protein 7 in the Development and Treatment of Bone Metastases from Breast Cancer

Author

Buijs, Jeroen T., primary, Henriquez, Nico V., primary, van Overveld, Petra G.M., primary, van der Horst, Geertje, primary, Que, Ivo, primary, Schwaninger, Ruth, primary, Rentsch, Cyrill, primary, ten Dijke, Peter, primary, Cleton-Jansen, Anne-Marie, primary, Driouch, Keltouma, primary, Lidereau, Rosette, primary, Bachelier, Richard, primary, Vukicevic, Slobodan, primary, Clézardin, Philippe, primary, Papapoulos, Socrates E., primary, Cecchini, Marco G., primary, Löwik, Clemens W.G.M., primary, and van der Pluijm, Gabri, primary

Published
2023
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17. Supplementary Table 1 from Bone Morphogenetic Protein 7 in the Development and Treatment of Bone Metastases from Breast Cancer

Author

Buijs, Jeroen T., primary, Henriquez, Nico V., primary, van Overveld, Petra G.M., primary, van der Horst, Geertje, primary, Que, Ivo, primary, Schwaninger, Ruth, primary, Rentsch, Cyrill, primary, ten Dijke, Peter, primary, Cleton-Jansen, Anne-Marie, primary, Driouch, Keltouma, primary, Lidereau, Rosette, primary, Bachelier, Richard, primary, Vukicevic, Slobodan, primary, Clézardin, Philippe, primary, Papapoulos, Socrates E., primary, Cecchini, Marco G., primary, Löwik, Clemens W.G.M., primary, and van der Pluijm, Gabri, primary

Published
2023
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18. Supplementary Table 2 from Bone Morphogenetic Protein 7 in the Development and Treatment of Bone Metastases from Breast Cancer

Author

Buijs, Jeroen T., primary, Henriquez, Nico V., primary, van Overveld, Petra G.M., primary, van der Horst, Geertje, primary, Que, Ivo, primary, Schwaninger, Ruth, primary, Rentsch, Cyrill, primary, ten Dijke, Peter, primary, Cleton-Jansen, Anne-Marie, primary, Driouch, Keltouma, primary, Lidereau, Rosette, primary, Bachelier, Richard, primary, Vukicevic, Slobodan, primary, Clézardin, Philippe, primary, Papapoulos, Socrates E., primary, Cecchini, Marco G., primary, Löwik, Clemens W.G.M., primary, and van der Pluijm, Gabri, primary

Published
2023
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19. Supplementary Methods and Materials from Bone Morphogenetic Protein 7 in the Development and Treatment of Bone Metastases from Breast Cancer

Author

Buijs, Jeroen T., primary, Henriquez, Nico V., primary, van Overveld, Petra G.M., primary, van der Horst, Geertje, primary, Que, Ivo, primary, Schwaninger, Ruth, primary, Rentsch, Cyrill, primary, ten Dijke, Peter, primary, Cleton-Jansen, Anne-Marie, primary, Driouch, Keltouma, primary, Lidereau, Rosette, primary, Bachelier, Richard, primary, Vukicevic, Slobodan, primary, Clézardin, Philippe, primary, Papapoulos, Socrates E., primary, Cecchini, Marco G., primary, Löwik, Clemens W.G.M., primary, and van der Pluijm, Gabri, primary

Published
2023
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21. Immune-mediated thrombotic thrombocytopenic purpura plasma induces calcium- and IgG-dependent endothelial activation: correlations with disease severity

Author

Tellier, Edwige, primary, Widemann, Agnès, additional, Cauchois, Raphaël, additional, Faccini, Julien, additional, Lagarde, Marie, additional, Brun, Marion, additional, Robert, Philippe, additional, Robert, Stéphane, additional, Bachelier, Richard, additional, Poullin, Pascale, additional, Roose, Elien, additional, Vanhoorelbeke, Karen, additional, Coppo, Paul, additional, Dignat-George, Françoise, additional, and Kaplanski, Gilles, additional

Published
2022
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22. Accelerated senescence of cord blood endothelial progenitor cells in premature neonates is driven by SIRT1 decreased expression

Author

Vassallo, Paula Frizera, Simoncini, Stéphanie, Ligi, Isabelle, Chateau, Anne-Line, Bachelier, Richard, Robert, Stéphane, Morere, Julia, Fernandez, Samantha, Guillet, Benjamin, Marcelli, Maxime, Tellier, Edwige, Pascal, Alain, Simeoni, Umberto, Anfosso, Francine, Magdinier, Frédérique, Dignat-George, Françoise, and Sabatier, Florence

Published
2014
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24. Involvement of Multiple Variants of Soluble CD146 in Systemic Sclerosis: Identification of a Novel Profibrotic Factor

Author

Nollet, Marie, primary, Bachelier, Richard, additional, Joshkon, Ahmad, additional, Traboulsi, Waël, additional, Mahieux, Amandine, additional, Moyon, Anais, additional, Muller, Alexandre, additional, Somasundaram, Indumathi, additional, Simoncini, Stéphanie, additional, Peiretti, Franck, additional, Leroyer, Aurélie S., additional, Guillet, Benjamin, additional, Granel, Brigitte, additional, Dignat‐George, Françoise, additional, Bardin, Nathalie, additional, Foucault‐Bertaud, Alexandrine, additional, and Blot‐Chabaud, Marcel, additional

Published
2022
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25. Soluble CD146 as a Potential Target for Preventing Triple Negative Breast Cancer MDA-MB-231 Cell Growth and Dissemination

Author

Sharma, Akshita, Joshkon, Ahmad, Ladjimi, Aymen, Traboulsi, Waël, Bachelier, Richard, Robert, Stéphane, Foucault-Bertaud, Alexandrine, Leroyer, Aurélie S., Bardin, Nathalie, Somasundaram, Indumathi, Blot-Chabaud, Marcel, D.Y. Patil University , Kolhapur, India, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Lucas, Nelly

Subjects
Epithelial-Mesenchymal Transition, QH301-705.5, Triple Negative Breast Neoplasms, CD146 Antigen, Article, Mice, Antineoplastic Agents, Immunological, Cell Line, Tumor, Biomarkers, Tumor, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Biology (General), QD1-999, Cell Proliferation, treatment, Cell Cycle, Xenograft Model Antitumor Assays, Up-Regulation, Gene Expression Regulation, Neoplastic, Chemistry, CD146, triple negative breast cancer, Neoplastic Stem Cells, Female
Abstract

Background: Triple Negative Breast Cancers (TNBC) are the most aggressive breast cancers and lead to poor prognoses. This is due to a high resistance to therapies, mainly because of the presence of Cancer Stem Cells (CSCs). Plasticity, a feature of CSCs, is acquired through the Epithelial to Mesenchymal Transition (EMT), a process that has been recently shown to be regulated by a key molecule, CD146. Of interest, CD146 is over-expressed in TNBC. Methods: The MDA-MB-231 TNBC cell line was used as a model to study the role of CD146 and its secreted soluble form (sCD146) in the development and dissemination of TNBC using in vitro and in vivo studies. Results: High expression of CD146 in a majority of MDA-MB-231 cells leads to an increased secretion of sCD146 that up-regulates the expression of EMT and CSC markers on the cells. These effects can be blocked with a specific anti-sCD146 antibody, M2J-1 mAb. M2J-1 mAb was able to reduce tumour development and dissemination in a model of cells xenografted in nude mice and an experimental model of metastasis, respectively, in part through its effects on CSC. Conclusion: We propose that M2J-1 mAb could be used as an additional therapeutic approach to fight TNBC.

Published
2022
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26. Additional file 1 of Soluble CD146, a biomarker and a target for preventing resistance to anti-angiogenic therapy in glioblastoma

Author

Joshkon, Ahmad, Tabouret, Emeline, Traboulsi, Wael, Bachelier, Richard, Simoncini, Stéphanie, Roffino, Sandrine, Jiguet-Jiglaire, Carine, Badran, Bassam, Guillet, Benjamin, Foucault-Bertaud, Alexandrine, Leroyer, Aurelie S., Dignat-George, Françoise, Chinot, Olivier, Fayyad-Kazan, Hussein, Bardin, Nathalie, and Blot-Chabaud, Marcel

Abstract

Additional file 1. Supplementary methods:Peptides and antibodies. Plasmids, siRNA and cell transfection. Western-blot and Immunoprecipitation assays. ELISA experiments. Immunofluorescence experiments. Flow cytometry experiments. Reverse Transcription-quantitative PCR (RT-qPCR). Cell migration assays. Cell Proliferation assays. Transwell invasion assays. Crispr/Cas9 deletion of genes. Experiments on animals and imaging. Immunohistochemistry. Patient cohort. Statistical analysis. Supplementary figures and tables: Supplementary Table 1: Absence of significant impact of age, KPS and steroid dose on progression-free survival or overall survival. Supplementary Table 2: references and application condition of the different antibodies used in the study. Supplementary Figure 1: Expression of CD146 on various types of cancers and effect on overall survival in patients with GBM. Supplementary Figure 2: Glioblastoma cell lines characterization. Supplementary Figure 3: Concentration of VEGF and sCD146 in the culture media of U87, U373, and U118 glioblastoma cell lines. Supplementary Figure 4: Avastin induces proliferation, sCD146 secretion, and EMT/CSC markers in U373 cells. Supplementary Figure 5: Avastin has no effect on CD146-negative U118 glioblastoma cells. Supplementary Figure 6: sCD146 induces U373 cell proliferation, migration and invasion in-vitro and promotes CSC and EMT markers. Supplementary Figure 7: Effect of sCD146 and VEGF on CD146-negative glioblastoma cells. Supplementary Figure 8: sCD146 binds integrin αvβ3 on U373 cells. Supplementary Figure 9: Soluble CD146 induces EMT in U118 cells transfected with integrin αvβ3. Supplementary Figure 10: Knocking-down integrin αvβ3 inhibits sCD146-induced EMT in U87 cells. Supplementary Figure 11: sCD146 mediated its effects on U87 cells through a signalosome containing CD146, αvβ3, and VEGFR2. Supplementary Figure 12: sCD146 mediates its effects on U373 cells through a signalosome containing CD146, αvβ3, and VEGFR2. Supplementary Figure 13: Validating gene knock out in U87 and U373 cells. Supplementary Figure 14: Integrin αvβ3 associates with membrane CD146 and VEGFR2 on HUVECs and binds sCD146. Supplementary Figure 15: CD146/VEGFR2/integrin αvβ3 signalosome mediates sCD146 and VEGF effects in U87 cells and activates common signaling pathways. Supplementary Figure 16: CD146/VEGFR2/integrin αvβ3 signalosome mediates sCD146 and VEGF effects in U373 cells and activates common signaling pathways. Supplementary Figure 17: CD146, integrin αvβ3, and VEGFR2 are essential for mediating sCD146 and VEGF effects on U87 cells. Supplementary Figure 18: CD146, integrin αvβ3, and VEGFR2 are essential for mediating sCD146 and VEGF effects on U373 cells. Supplementary Figure 19: Humanized anti-sCD146 antibody mucizumab significantly decreases U373 cell proliferation, migration and invasion and hampers CSC and EMT in-vitro. Supplementary Figure 20: Humanized anti-sCD146 mucizumab significantly decreases human sCD146 and human VEGF in two different mouse models of glioblastoma. Supplementary Figure 21: Illustrative summary describing the mechanism of resistance to bevacizumab in CD146-positive glioblastoma cells.

Published
2022
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28. Multilineage Differentiation for Formation of Innervated Skeletal Muscle Fibers from Healthy and Diseased Human Pluripotent Stem Cells

Author

Mazaleyrat, Kilian, Badja, Cherif, Broucqsault, Natacha, Chevalier, Raphaël, Laberthonnière, Camille, Dion, Camille, Baldasseroni, Lyla, El-Yazidi, Claire, Thomas, Morgane, Bachelier, Richard, Altié, Alexandre, Nguyen, Karine, Levy, Nicolas, ROBIN, Jérôme, Magdinier, Frédérique, Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département de génétique médicale [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Region Provence Alpes Cote d'Azur (Cladimus project) 2018-06436Excellence Initiative of Aix-Marseille University-A*Midex, a French 'investissement d'avenir programme' through Marseille Maladies Rares (MarMaRa) Institute AMX-19-IET-007, Bachelier, Richard [0000-0002-0236-2633], Robin, Jérôme D [0000-0001-5865-8372], Magdinier, Frédérique [0000-0002-0159-9559], Apollo - University of Cambridge Repository, and ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011)

Subjects
Pluripotent Stem Cells, human induced pluripotent cells, satellite cells, muscular dystrophy, Facio-Scapulo-Humeral Dystrophy, Muscle Fibers, Skeletal, myoblasts, contraction, Cell Differentiation, differentiation, Duchenne Muscular Dystrophy, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Article, Muscular Dystrophy, Duchenne, Limb girdle muscular dystrophy, lcsh:Biology (General), myotubes, Z-lines, Humans, motor neurons, Myotonic Dystrophy, lcsh:QH301-705.5
Abstract

Induced pluripotent stem cells (iPSCs) obtained by reprogramming primary somatic cells have revolutionized the fields of cell biology and disease modeling. However, the number protocols for generating mature muscle fibers with sarcolemmal organization using iPSCs remain limited, and partly mimic the complexity of mature skeletal muscle. Methods: We used a novel combination of small molecules added in a precise sequence for the simultaneous codifferentiation of human iPSCs into skeletal muscle cells and motor neurons. Results: We show that the presence of both cell types reduces the production time for millimeter-long multinucleated muscle fibers with sarcolemmal organization. Muscle fiber contractions are visible in 19&ndash, 21 days, and can be maintained over long period thanks to the production of innervated multinucleated mature skeletal muscle fibers with autonomous cell regeneration of PAX7-positive cells and extracellular matrix synthesis. The sequential addition of specific molecules recapitulates key steps of human peripheral neurogenesis and myogenesis. Furthermore, this organoid-like culture can be used for functional evaluation and drug screening. Conclusion: Our protocol, which is applicable to hiPSCs from healthy individuals, was validated in Duchenne Muscular Dystrophy, Myotonic Dystrophy, Facio-Scapulo-Humeral Dystrophy and type 2A Limb-Girdle Muscular Dystrophy, opening new paths for the exploration of muscle differentiation, disease modeling and drug discovery.

Published
2020
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34. CD146/sCD146 in the Pathogenesis and Monitoring of Angiogenic and Inflammatory Diseases

Author

Heim, Xavier, Joshkon, Ahmad, Bermudez, Julien, Bachelier, Richard, Dubrou, Cléa, Boucraut, José, Foucault-Bertaud, Alexandrine, Leroyer, Aurélie, Dignat-George, Francoise, Blot-Chabaud, Marcel, Bardin, Nathalie, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital de la Timone [CHU - APHM] (TIMONE), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Hôpital Nord [CHU - APHM], Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Immunologie [Hôpital de la Conception - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION)-Centre National de la Recherche Scientifique (CNRS), Vascular research center of Marseille (VRCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Lucas, Nelly, and Leroyer, Aurelie

Subjects
[SDV] Life Sciences [q-bio], soluble CD146, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, lcsh:Biology (General), CD146, inflammation angiogenesis, [SDV]Life Sciences [q-bio], autoimmunity, Review, lcsh:QH301-705.5, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; CD146 is a cell adhesion molecule expressed on endothelial cells, as well as on other cells such as mesenchymal stem cells and Th17 lymphocytes. This protein also exists in a soluble form, whereby it can be detected in biological fluids, including the serum or the cerebrospinal fluid (CSF). Some studies have highlighted the significance of CD146 and its soluble form in angiogenesis and inflammation, having been shown to contribute to the pathogenesis of many inflammatory autoimmune diseases, such as systemic sclerosis, mellitus diabetes, rheumatoid arthritis, inflammatory bowel diseases, and multiple sclerosis. In this review, we will focus on how CD146 and sCD146 contribute to the pathogenesis of the aforementioned autoimmune diseases and discuss the relevance of considering it as a biomarker in these pathologies.

Published
2020
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38. Role of CD146 (MCAM) in Physiological and Pathological Angiogenesis—Contribution of New Antibodies for Therapy

Author

Joshkon, Ahmad, primary, Heim, Xavier, additional, Dubrou, Cléa, additional, Bachelier, Richard, additional, Traboulsi, Wael, additional, Stalin, Jimmy, additional, Fayyad-Kazan, Hussein, additional, Badran, Bassam, additional, Foucault-Bertaud, Alexandrine, additional, Leroyer, Aurelie S., additional, Bardin, Nathalie, additional, and Blot-Chabaud, Marcel, additional

Published
2020
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39. Therapeutic targeting of soluble CD146/MCAM with the M2J‐1 monoclonal antibody prevents metastasis development and procoagulant activity in CD146‐positive invasive tumors

Author

Stalin, Jimmy, primary, Traboulsi, Wael, additional, Vivancos‐Stalin, Lucie, additional, Nollet, Marie, additional, Joshkon, Ahmad, additional, Bachelier, Richard, additional, Guillet, Benjamin, additional, Lacroix, Romaric, additional, Foucault‐Bertaud, Alexandrine, additional, Leroyer, Aurélie S., additional, Dignat‐George, Françoise, additional, Bardin, Nathalie, additional, and Blot‐Chabaud, Marcel, additional

Published
2020
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40. A novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule

Author

Nollet, Marie, STALIN, JIMMY, Moyon, Anaïs, Traboulsi, Wael, Essaadi, Amel, Robert, Stéphane, Malissen, Nausicaa, Bachelier, Richard, Daniel, Laurent, Foucault-Bertaud, Alexandrine, Gaudy-Marqueste, Caroline, Lacroix, Romaric, Leroyer, Aurélie, Guillet, Benjamin, Bardin, Nathalie, Dignat-George, Francoise, Blot-Chabaud, Marcel, Vascular research center of Marseille (VRCM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie de l'Endothelium, Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aix-Marseille Université - Faculté de pharmacie (AMU PHARM), Aix Marseille Université (AMU), Département de dermatologie [CHU de Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Hôpital de la Timone [CHU - APHM] (TIMONE), Département de dermatologie, CHU Marseille, CHU Marseille, Laboratoire d'Immunologie [Hôpital de la Conception - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), and Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )

Subjects
therapy, PET, CD146, antibody, [SDV]Life Sciences [q-bio], cancer, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; CD146 is an adhesion molecule present on many tumors (melanoma, kidney, pancreas, breast, ...). In addition, it has been shown to be expressed on vascular endothelial and smooth muscle cells. Generating an antibody able to specifically recognize CD146 in cancer cells (designated as tumor CD146), but not in normal cells, would thus be of major interest for targeting tumor CD146 without affecting the vascular system. We thus generated antibodies against the extracellular domain of the molecule produced in cancer cells and selected an antibody that specifically recognizes tumor CD146. This antibody (TsCD146 mAb) was able to detect CD146-positive tumors in human biopsies and in vivo, by PET imaging, in a murine xenograft model. In addition, TsCD146 mAb antibody was able to specifically detect CD146-positive cancer microparticles in the plasma of patients. TsCD146 mAb displayed also therapeutic effects since it was able to reduce the growth of human CD146-positive cancer cells xenografted in nude mice. This effect was due to a decrease in the proliferation and an increase in the apoptosis of CD146-positive cancer cells after TsCD146-mediated internalization of the cell surface CD146. Thus, TsCD146 mAb could be of major interest for diagnostic and therapeutic strategies against CD146-positive tumors in a context of personalized medicine. www.impactjournals.com/oncotarget/

Published
2017
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41. miRNA-30 Family Members Inhibit Breast Cancer Invasion, Osteomimicry, and Bone Destruction by Directly Targeting Multiple Bone Metastasis–Associated Genes

Author

Croset, Martine, primary, Pantano, Francesco, additional, Kan, Casina W.S., additional, Bonnelye, Edith, additional, Descotes, Françoise, additional, Alix-Panabières, Catherine, additional, Lecellier, Charles-Henri, additional, Bachelier, Richard, additional, Allioli, Nathalie, additional, Hong, Saw-See, additional, Bartkowiak, Kai, additional, Pantel, Klaus, additional, and Clézardin, Philippe, additional

Published
2018
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42. Abstract 486: MicroRNA-30 family members inhibit breast cancer invasion, osteomimicry, and bone destruction by directly targeting multiple bone metastasis-associated genes

Author

Croset, Martine, primary, Pantano, Francesco, additional, Kan, Casina, additional, Bonnelye, Edith, additional, Descotes, Francoise, additional, Alix-Panabières, Catherine, additional, Lecellier, Charles, additional, Bachelier, Richard, additional, Allioli, Nathalie, additional, Hong, Saw See, additional, Bartkowiak, Kai, additional, Pantel, Klaus, additional, and Clézardin, Philippe, additional

Published
2018
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43. Biogenesis of Pro-senescent Microparticles by Endothelial Colony Forming Cells from Premature Neonates is driven by SIRT1-Dependent Epigenetic Regulation of MKK6

Author

Simoncini, Stéphanie, primary, Chateau, Anne-Line, additional, Robert, Stéphane, additional, Todorova, Dilyana, additional, Yzydorzick, Catherine, additional, Lacroix, Romaric, additional, Ligi, Isabelle, additional, Louis, Laurence, additional, Bachelier, Richard, additional, Simeoni, Umberto, additional, Magdinier, Frédérique, additional, Dignat-George, Françoise, additional, and Sabatier, Florence, additional

Published
2017
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44. CD146 mediates VEGF-induced melanoma cell extravasation through FAK activation

Author

Leroyer, Aurelie, Jouve, Nathalie, Bachelier, Richard, Despoix, Nicolas, Blin, Muriel, Matinzadeh, Maryam Khalili, Poitevin, Stéphane, Aurrand-Lions, Michel, Fallague, Karim, Bardin, Nathalie, Blot-Chabaud, Marcel, Vely, Frédéric, Dignat-George, Françoise, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015
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45. Soluble CD146 boosts therapeutic effect of endothelial progenitors through proteolytic processing of short CD146 isoform

Author

Stalin, Jimmy, primary, Harhouri, Karim, additional, Hubert, Lucas, additional, Garrigue, Philippe, additional, Nollet, Marie, additional, Essaadi, Amel, additional, Muller, Alexandre, additional, Foucault-Bertaud, Alexandrine, additional, Bachelier, Richard, additional, Sabatier, Florence, additional, Pisano, Pascale, additional, Peiretti, Franck, additional, Leroyer, Aurélie S., additional, Guillet, Benjamin, additional, Bardin, Nathalie, additional, Dignat-George, Françoise, additional, and Blot-Chabaud, Marcel, additional

Published
2016
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46. Abstract 437: Cd146 Deficiency Leads to Accelerated Atherosclerosis in Mice Through Upregulation of Rantes

Author

Blin, Muriel G, primary, Bachelier, Richard, additional, Fernandez, Samantha, additional, Guillet, Benjamin, additional, Fallague, Karim, additional, Robert, Stéphane, additional, Heymes, Christophe, additional, Bardin, Nathalie, additional, Blot-Chabaud, Marcel, additional, Dignat-George, Francoise, additional, and Leroyer, Aurélie S, additional

Published
2016
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48. CD146 mediates VEGF-induced melanoma cell extravasation through FAK activation

Author

Jouve, Nathalie, primary, Bachelier, Richard, additional, Despoix, Nicolas, additional, Blin, Muriel G., additional, Matinzadeh, Maryam Khalili, additional, Poitevin, Stéphane, additional, Aurrand-Lions, Michel, additional, Fallague, Karim, additional, Bardin, Nathalie, additional, Blot-Chabaud, Marcel, additional, Vely, Frédéric, additional, Dignat-George, Françoise, additional, and Leroyer, Aurélie S., additional

Published
2014
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