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Your search keyword '"Bae Seon Joo"' showing total 7 results
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7 results on '"Bae Seon Joo"'

1. ANKRD1 Promotes Breast Cancer Metastasis by Activating NF- κ B-MAGE-A6 Pathway.

Author

Diskul-Na-Ayudthaya, Penchatr, Bae, Seon Joo, Bae, Yun-Ui, Van, Ngu Trinh, Kim, Wootae, and Ryu, Seongho

Subjects
*PROTEINS, *NF-kappa B, *IN vitro studies, *RESEARCH funding, *SURVIVAL rate, *BREAST tumors, *EARLY detection of cancer, *CELL motility, *CELLULAR signal transduction, *TREATMENT effectiveness, *IN vivo studies, *TUMOR markers, *METASTASIS, *CELL lines
Abstract

Simple Summary: Change in protein expression is the key driving force for tumorigenesis. Increased expression of Ankyrin Repeat Domain 1 (ANKRD1) acts as a co-activator of the p53 tumor suppressor protein and is associated with cancer drug resistance and poor survival of cancer patients. In this study, we found increased levels of ANKRD1 in highly metastatic breast cancer cell lines and human tissues of high-grade breast cancer. We also demonstrated that ANKRD1 is upstream of NF-κB-MAGE-A6 and plays a role in cancer metastasis. Our results show that the expression of ANKRD1 has significant clinical diagnostic significance in breast cancer metastasis. Early detection and surgical excision of tumors have helped improve the survival rate of patients with breast cancer. However, patients with metastatic cancer typically have a poor prognosis. In this study, we propose that ANKRD1 promotes metastasis of breast cancer. ANKRD1 was found to be highly expressed in the MDA-MB-231 and MDA-LM-2 highly metastatic breast cancer cell lines compared to the non-metastatic breast cancer cell lines (MCF-7, ZR-75-30, T47D) and normal breast cancer cells (MCF-10A). Furthermore, high-grade tumors showed increased levels of ANKRD1 compared to low-grade tumors. Both in vitro and in vivo functional studies demonstrated the essential role of ANKRD1 in cancer cell migration and invasion. The previous studies have suggested a significant role of NF-κB and MAGE-A6 in breast cancer metastasis, but the upstream regulators of this axis are not well characterized. Our study suggests that ANKRD1 promotes metastasis of breast cancer by activating NF-κB as well as MAGE-A6 signaling. Our findings show that ANKRD1 is a potential therapeutic target and a diagnostic marker for breast cancer metastasis. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Self-assembled Micelle Interfering RNA for Effective and Safe Targeting of Dysregulated Genes in Pulmonary Fibrosis

Author

Won-Kyung Cho, Han Oh Park, Chun Geun Lee, Qing Lu, Kyuhong Lee, Jin Wook Park, Pyoung Oh Yoon, Han Na Kim, Sung-Hwan Kim, Hye Ryun Kang, Jun Hong Park, Joo Sung Yang, Jack A. Elias, Sung-Il Yun, Woo-Seok Kim, Youngho Ko, Chang-Min Lee, Bae Seon Joo, Tae-woo Kwon, and Ji-Young Lee

Subjects
0301 basic medicine, Male, Small interfering RNA, EGF Family of Proteins, medicine.medical_treatment, Pulmonary Fibrosis, Connective tissue, Biology, Bleomycin, Biochemistry, Amphiregulin, 03 medical and health sciences, chemistry.chemical_compound, In vivo, Pulmonary fibrosis, medicine, Gene silencing, Animals, Tissue Distribution, Gene Regulation, RNA, Small Interfering, Molecular Biology, Lung, Cells, Cultured, Micelles, Growth factor, Connective Tissue Growth Factor, Cell Biology, Genetic Therapy, medicine.disease, Molecular biology, Cell biology, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, chemistry, Gene Knockdown Techniques, Nanoparticles, Female, RNA Interference, Collagen
Abstract

The siRNA silencing approach has long been used as a method to regulate the expression of specific target genes in vitro and in vivo. However, the effectiveness of delivery and the nonspecific immune-stimulatory function of siRNA are the limiting factors for therapeutic applications of siRNAs. To overcome these limitations, we developed self-assembled micelle inhibitory RNA (SAMiRNA) nanoparticles made of individually biconjugated siRNAs with a hydrophilic polymer and lipid on their ends and characterized their stability, immune-stimulatory function, and in vivo silencing efficacy. SAMiRNAs form very stable nanoparticles with no significant degradation in size distribution and polydispersity index over 1 year. Overnight incubation of SAMiRNAs (3 μm) on murine peripheral blood mononuclear cells did not cause any significant elaboration of innate immune cytokines such as TNF-α, IL-12, or IL-6, whereas unmodified siRNAs or liposomes or liposome complexes significantly stimulated the expression of these cytokines. Last, the in vivo silencing efficacy of SAMiRNAs was evaluated by targeting amphiregulin and connective tissue growth factor in bleomycin or TGF-β transgenic animal models of pulmonary fibrosis. Intratracheal or intravenous delivery two or three times of amphiregulin or connective tissue growth factor SAMiRNAs significantly reduced the bleomycin- or TGF-β-stimulated collagen accumulation in the lung and substantially restored the lung function of TGF-β transgenic mice. This study demonstrates that SAMiRNA nanoparticle is a less toxic, stable siRNA silencing platform for efficient in vivo targeting of genes implicated in the pathogenesis of pulmonary fibrosis.

Published
2015

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