20 results on '"Baeseman, J."'
Search Results
2. Antarctic Climate Change and the Environment - 2017 Update
- Author
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Turner, J., Summerhayes, C., Bracegirdle, T., Liggett, D., Baeseman, J., Mayewski, P., Convey, P., di Prisco, G., Gutt, Julian, Hodgson, D., Worby, T., Wang, Z., and Klepikov, A.
- Published
- 2017
3. Delievering 21st century Antarctic and Southern Ocean science
- Author
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Kennicutt II, M. C., Kim, Y. D., Rogan-Finnemore, M., Anandakrishnan, S., Chown, S. L., Colwell, S. R., Cowan, D., Escutia, C., Frenot, Y., Hall, J., Liggett, D., McDonald, A., Nixdorf, Uwe, Siegert, M. J., Storey, J., Wahlin, A., Weatherwax, A., Wilson, G. S., Wooding, R., Ackley, S., Biebow, Nicole, Blankenship, D., Bo, S., Baeseman, J., Cardenas, C. A., Cassano, J., Danhong, C., Danobeitia, J., Francis, J., Guldahl, J., Hashida, G., Jimenez Corbalan, L., Klepikov, A., Lee, J., Leppe, M., Lijun, F., López-Martinez, J., Memolli, M., Motoyoshi, Y., Mousalle Bueno, R., Negrete, J., Ojeda Cardenes, M.A., Proano Silva, M., Ramos-Garcia, S., Sala, H., Shin, H., Shije, X., Shiraishi, K., Stockings, T., Trotter, S., Vaughan, D. G., Viera Da Unha De Menezes, J., Vlasich, V., Weijia, Q., Winther, J.-G., Miller, Heinrich, Rintoul, S., Yang, H., Kennicutt II, M. C., Kim, Y. D., Rogan-Finnemore, M., Anandakrishnan, S., Chown, S. L., Colwell, S. R., Cowan, D., Escutia, C., Frenot, Y., Hall, J., Liggett, D., McDonald, A., Nixdorf, Uwe, Siegert, M. J., Storey, J., Wahlin, A., Weatherwax, A., Wilson, G. S., Wooding, R., Ackley, S., Biebow, Nicole, Blankenship, D., Bo, S., Baeseman, J., Cardenas, C. A., Cassano, J., Danhong, C., Danobeitia, J., Francis, J., Guldahl, J., Hashida, G., Jimenez Corbalan, L., Klepikov, A., Lee, J., Leppe, M., Lijun, F., López-Martinez, J., Memolli, M., Motoyoshi, Y., Mousalle Bueno, R., Negrete, J., Ojeda Cardenes, M.A., Proano Silva, M., Ramos-Garcia, S., Sala, H., Shin, H., Shije, X., Shiraishi, K., Stockings, T., Trotter, S., Vaughan, D. G., Viera Da Unha De Menezes, J., Vlasich, V., Weijia, Q., Winther, J.-G., Miller, Heinrich, Rintoul, S., and Yang, H.
- Abstract
The Antarctic Roadmap Challenges (ARC) project identified critical requirements to deliver high priority Antarctic research in the 21st century. The ARC project addressed the challenges of enabling technologies, facilitating access, providing logistics and infrastructure, and capitalizing on international co-operation. Technological requirements include: i) innovative automated in situ observing systems, sensors and interoperable platforms (including power demands), ii) realistic and holistic numerical models, iii) enhanced remote sensing and sensors, iv) expanded sample collection and retrieval technologies, and v) greater cyber-infrastructure to process ‘big data’ collection, transmission and analyses while promoting data accessibility. These technologies must be widely available, performance and reliability must be improved and technologies used elsewhere must be applied to the Antarctic. Considerable Antarctic research is field-based, making access to vital geographical targets essential. Future research will require continent- and ocean-wide environmentally responsible access to coastal and interior Antarctica and the Southern Ocean. Year-round access is indispensable. The cost of future Antarctic science is great but there are opportunities for all to participate commensurate with national resources, expertise and interests. The scope of future Antarctic research will necessitate enhanced and inventive interdisciplinary and international collaborations. The full promise of Antarctic science will only be realized if nations act together.
- Published
- 2016
4. Delivering 21st century Antarctic and Southern Ocean science
- Author
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Kennicutt, M.C., Kim, Y.D., Rogan-Finnemore, M., Anandakrishnan, S., Chown, S.L., Colwell, S., Cowan, D., Escutia, C., Frenot, Y., Hall, J., Liggett, D., Mcdonald, A.J., Nixdorf, U., Siegert, M.J., Storey, J., Wåhlin, A., Weatherwax, A., Wilson, G.S., Wilson, T., Wooding, R., Ackley, S., Biebow, N., Blankenship, D., Bo, S., Baeseman, J., Cárdenas, C.A., Cassano, J., Danhong, C., Dañobeitia, J., Francis, J., Guldahl, J., Hashida, G., Corbalán, L. Jiménez, Klepikov, A., Lee, J., Leppe, M., Lijun, F., López-Martinez, J., Memolli, M., Motoyoshi, Y., Bueno, R. Mousalle, Negrete, J., Cárdenes, M.A. Ojeda, Silva, M. Proaño, Ramos-Garcia, S., Sala, H., Shin, H., Shijie, X., Shiraishi, K., Stockings, T., Trotter, S., Vaughan, D.G., De Menezes, J. Viera Da Unha, Vlasich, V., Weijia, Q., Winther, J.-G., Miller, H., Rintoul, S., Yang, H., Kennicutt, M.C., Kim, Y.D., Rogan-Finnemore, M., Anandakrishnan, S., Chown, S.L., Colwell, S., Cowan, D., Escutia, C., Frenot, Y., Hall, J., Liggett, D., Mcdonald, A.J., Nixdorf, U., Siegert, M.J., Storey, J., Wåhlin, A., Weatherwax, A., Wilson, G.S., Wilson, T., Wooding, R., Ackley, S., Biebow, N., Blankenship, D., Bo, S., Baeseman, J., Cárdenas, C.A., Cassano, J., Danhong, C., Dañobeitia, J., Francis, J., Guldahl, J., Hashida, G., Corbalán, L. Jiménez, Klepikov, A., Lee, J., Leppe, M., Lijun, F., López-Martinez, J., Memolli, M., Motoyoshi, Y., Bueno, R. Mousalle, Negrete, J., Cárdenes, M.A. Ojeda, Silva, M. Proaño, Ramos-Garcia, S., Sala, H., Shin, H., Shijie, X., Shiraishi, K., Stockings, T., Trotter, S., Vaughan, D.G., De Menezes, J. Viera Da Unha, Vlasich, V., Weijia, Q., Winther, J.-G., Miller, H., Rintoul, S., and Yang, H.
- Abstract
The Antarctic Roadmap Challenges (ARC) project identified critical requirements to deliver high priority Antarctic research in the 21st century. The ARC project addressed the challenges of enabling technologies, facilitating access, providing logistics and infrastructure, and capitalizing on international co-operation. Technological requirements include: i) innovative automated in situ observing systems, sensors and interoperable platforms (including power demands), ii) realistic and holistic numerical models, iii) enhanced remote sensing and sensors, iv) expanded sample collection and retrieval technologies, and v) greater cyber-infrastructure to process ‘big data’ collection, transmission and analyses while promoting data accessibility. These technologies must be widely available, performance and reliability must be improved and technologies used elsewhere must be applied to the Antarctic. Considerable Antarctic research is field-based, making access to vital geographical targets essential. Future research will require continent- and ocean-wide environmentally responsible access to coastal and interior Antarctica and the Southern Ocean. Year-round access is indispensable. The cost of future Antarctic science is great but there are opportunities for all to participate commensurate with national resources, expertise and interests. The scope of future Antarctic research will necessitate enhanced and inventive interdisciplinary and international collaborations. The full promise of Antarctic science will only be realized if nations act together.
- Published
- 2016
5. Permafrost Research Priorities: A roadmap for the future of permafrost research
- Author
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Lantuit, H., Allard, M., Guglielmin, M., Johansson, M., Kraev, G., Krautblatter, M., Krinner, G., Schuur, E., Sjöberg, Y., Baeseman, J., and Schollaen, K.
- Published
- 2014
6. Community review of Southern Ocean satellite data needs
- Author
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Pope, A., primary, Wagner, P., additional, Johnson, R., additional, Shutler, J.D., additional, Baeseman, J., additional, and Newman, L., additional
- Published
- 2016
- Full Text
- View/download PDF
7. Delivering 21st century Antarctic and Southern Ocean science
- Author
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Kennicutt, M.C., primary, Kim, Y.D., additional, Rogan-Finnemore, M., additional, Anandakrishnan, S., additional, Chown, S.L., additional, Colwell, S., additional, Cowan, D., additional, Escutia, C., additional, Frenot, Y., additional, Hall, J., additional, Liggett, D., additional, Mcdonald, A.J., additional, Nixdorf, U., additional, Siegert, M.J., additional, Storey, J., additional, Wåhlin, A., additional, Weatherwax, A., additional, Wilson, G.S., additional, Wilson, T., additional, Wooding, R., additional, Ackley, S., additional, Biebow, N., additional, Blankenship, D., additional, Bo, S., additional, Baeseman, J., additional, Cárdenas, C.A., additional, Cassano, J., additional, Danhong, C., additional, Dañobeitia, J., additional, Francis, J., additional, Guldahl, J., additional, Hashida, G., additional, Corbalán, L. Jiménez, additional, Klepikov, A., additional, Lee, J., additional, Leppe, M., additional, Lijun, F., additional, López-Martinez, J., additional, Memolli, M., additional, Motoyoshi, Y., additional, Bueno, R. Mousalle, additional, Negrete, J., additional, Cárdenes, M.A. Ojeda, additional, Silva, M. Proaño, additional, Ramos-Garcia, S., additional, Sala, H., additional, Shin, H., additional, Shijie, X., additional, Shiraishi, K., additional, Stockings, T., additional, Trotter, S., additional, Vaughan, D.G., additional, De Menezes, J. Viera Da Unha, additional, Vlasich, V., additional, Weijia, Q., additional, Winther, J.-G., additional, Miller, H., additional, Rintoul, S., additional, and Yang, H., additional
- Published
- 2016
- Full Text
- View/download PDF
8. A roadmap for Antarctic and Southern Ocean science for the next two decades and beyond
- Author
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Kennicutt, M.C., Chown, S.L., Cassano, J.J., Liggett, D., Peck, L.S., Massom, R., Rintoul, S.R., Storey, J., Vaughan, D.G., Wilson, T.J., Allison, I., Ayton, J., Badhe, R., Baeseman, J., Barrett, P.J., Bell, R.E., Bertler, N., Bo, S., Brandt, A., Bromwich, D., Cary, S.C., Clark, M.S., Convey, P., Costa, E.S., Cowan, D., Deconto, R., Dunbar, R., Elfring, C., Escutia, C., Francis, J., Fricker, H.A., Fukuchi, M., Gilbert, N., Gutt, J., Havermans, C., Hik, D., Hosie, G., Jones, C., Kim, Y.D., Le Maho, Y., Lee, S.H., Leppe, M., Leitchenkov, G., Li, X., Lipenkov, V., Lochte, K., López-Martínez, J., Lüdecke, C., Lyons, W., Marenssi, S., Miller, H., Morozova, P., Naish, T., Nayak, S., Ravindra, R., Retamales, J., Ricci, C.A., Rogan-Finnemore, M., Ropert-Coudert, Y., Samah, A.A., Sanson, L., Scambos, T., Schloss, I.R., Shiraishi, K., Siegert, M.J., Simões, J.C., Storey, B., Sparrow, M.D., Wall, D.H., Walsh, J.C., Wilson, G., Winther, J.G., Xavier, J.C., Yang, H., Sutherland, W.J., Kennicutt, M.C., Chown, S.L., Cassano, J.J., Liggett, D., Peck, L.S., Massom, R., Rintoul, S.R., Storey, J., Vaughan, D.G., Wilson, T.J., Allison, I., Ayton, J., Badhe, R., Baeseman, J., Barrett, P.J., Bell, R.E., Bertler, N., Bo, S., Brandt, A., Bromwich, D., Cary, S.C., Clark, M.S., Convey, P., Costa, E.S., Cowan, D., Deconto, R., Dunbar, R., Elfring, C., Escutia, C., Francis, J., Fricker, H.A., Fukuchi, M., Gilbert, N., Gutt, J., Havermans, C., Hik, D., Hosie, G., Jones, C., Kim, Y.D., Le Maho, Y., Lee, S.H., Leppe, M., Leitchenkov, G., Li, X., Lipenkov, V., Lochte, K., López-Martínez, J., Lüdecke, C., Lyons, W., Marenssi, S., Miller, H., Morozova, P., Naish, T., Nayak, S., Ravindra, R., Retamales, J., Ricci, C.A., Rogan-Finnemore, M., Ropert-Coudert, Y., Samah, A.A., Sanson, L., Scambos, T., Schloss, I.R., Shiraishi, K., Siegert, M.J., Simões, J.C., Storey, B., Sparrow, M.D., Wall, D.H., Walsh, J.C., Wilson, G., Winther, J.G., Xavier, J.C., Yang, H., and Sutherland, W.J.
- Abstract
Antarctic and Southern Ocean science is vital to understanding natural variability, the processes that govern global change and the role of humans in the Earth and climate system. The potential for new knowledge to be gained from future Antarctic science is substantial. Therefore, the international Antarctic community came together to ‘scan the horizon’ to identify the highest priority scientific questions that researchers should aspire to answer in the next two decades and beyond. Wide consultation was a fundamental principle for the development of a collective, international view of the most important future directions in Antarctic science. From the many possibilities, the horizon scan identified 80 key scientific questions through structured debate, discussion, revision and voting. Questions were clustered into seven topics: i) Antarctic atmosphere and global connections, ii) Southern Ocean and sea ice in a warming world, iii) ice sheet and sea level, iv) the dynamic Earth, v) life on the precipice, vi) near-Earth space and beyond, and vii) human presence in Antarctica. Answering the questions identified by the horizon scan will require innovative experimental designs, novel applications of technology, invention of next-generation field and laboratory approaches, and expanded observing systems and networks. Unbiased, non-contaminating procedures will be required to retrieve the requisite air, biota, sediment, rock, ice and water samples. Sustained year-round access to Antarctica and the Southern Ocean will be essential to increase winter-time measurements. Improved models are needed that represent Antarctica and the Southern Ocean in the Earth System, and provide predictions at spatial and temporal resolutions useful for decision making. A co-ordinated portfolio of cross-disciplinary science, based on new models of international collaboration, will be essential as no scientist, programme or nation can realize these aspirations alone.
- Published
- 2015
9. Climate Changes at the Poles: Research immersion experience at Bellingshausen, Antarctica
- Author
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Alexeev, V., Repina, I., Fernandoy, Francisco, and Baeseman, J.
- Abstract
This immersion experience exposed students from multiple disciplines and several nations to basic and advanced Antarctic research techniques necessary for understanding the area of the world most affected by climate change.This course offered a general overview of several fields, techniques and technologies used to collect and analyze data, hands-on training in multiple disciplines and incorporate research projects that use a systems approach and were enhanced by international perspectives on newly synthesized science hypotheses. The field school provided the opportunity and forum for collaborations between a number of scientists and young researchers from a variety of backgrounds. The students gained knowledge of one of the most rapidly warming region on Earth and sociological impacts. They also acquired hands-on field techniques for collecting data, data sharing and management. They learned logistical requirements for research at several international Antarctic stations and developed international and interdisciplinary research projects and collaborators. In addition, they will participate in disseminating this information and knowledge to other young researchers around the world. The abstract has been submitted while the summer school has been conducted in Antarctica.
- Published
- 2010
10. Community review of Southern Ocean satellite data needs.
- Author
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Pope, A., Wagner, P., Johnson, R., Shutler, J.D., Baeseman, J., and Newman, L.
- Subjects
SEA ice ,OCEAN temperature - Abstract
This review represents the Southern Ocean community’s satellite data needs for the coming decade. Developed through widespread engagement and incorporating perspectives from a range of stakeholders (both research and operational), it is designed as an important community-driven strategy paper that provides the rationale and information required for future planning and investment. The Southern Ocean is vast but globally connected, and the communities that require satellite-derived data in the region are diverse. This review includes many observable variables, including sea ice properties, sea surface temperature, sea surface height, atmospheric parameters, marine biology (both micro and macro) and related activities, terrestrial cryospheric connections, sea surface salinity, and a discussion of coincident and in situ data collection. Recommendations include commitment to data continuity, increases in particular capabilities (sensor types, spatial, temporal), improvements in dissemination of data/products/uncertainties, and innovation in calibration/validation capabilities. Full recommendations are detailed by variable as well as summarized. This review provides a starting point for scientists to understand more about Southern Ocean processes and their global roles, for funders to understand the desires of the community, for commercial operators to safely conduct their activities in the Southern Ocean, and for space agencies to gain greater impact from Southern Ocean-related acquisitions and missions. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
11. A roadmap for Antarctic and Southern Ocean science for the next two decades and beyond
- Author
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Kennicutt, M.C., primary, Chown, S.L., additional, Cassano, J.J., additional, Liggett, D., additional, Peck, L.S., additional, Massom, R., additional, Rintoul, S.R., additional, Storey, J., additional, Vaughan, D.G., additional, Wilson, T.J., additional, Allison, I., additional, Ayton, J., additional, Badhe, R., additional, Baeseman, J., additional, Barrett, P.J., additional, Bell, R.E., additional, Bertler, N., additional, Bo, S., additional, Brandt, A., additional, Bromwich, D., additional, Cary, S.C., additional, Clark, M.S., additional, Convey, P., additional, Costa, E.S., additional, Cowan, D., additional, Deconto, R., additional, Dunbar, R., additional, Elfring, C., additional, Escutia, C., additional, Francis, J., additional, Fricker, H.A., additional, Fukuchi, M., additional, Gilbert, N., additional, Gutt, J., additional, Havermans, C., additional, Hik, D., additional, Hosie, G., additional, Jones, C., additional, Kim, Y.D., additional, Le Maho, Y., additional, Lee, S.H., additional, Leppe, M., additional, Leitchenkov, G., additional, Li, X., additional, Lipenkov, V., additional, Lochte, K., additional, López-Martínez, J., additional, Lüdecke, C., additional, Lyons, W., additional, Marenssi, S., additional, Miller, H., additional, Morozova, P., additional, Naish, T., additional, Nayak, S., additional, Ravindra, R., additional, Retamales, J., additional, Ricci, C.A., additional, Rogan-Finnemore, M., additional, Ropert-Coudert, Y., additional, Samah, A.A., additional, Sanson, L., additional, Scambos, T., additional, Schloss, I.R., additional, Shiraishi, K., additional, Siegert, M.J., additional, Simões, J.C., additional, Storey, B., additional, Sparrow, M.D., additional, Wall, D.H., additional, Walsh, J.C., additional, Wilson, G., additional, Winther, J.G., additional, Xavier, J.C., additional, Yang, H., additional, and Sutherland, W.J., additional
- Published
- 2014
- Full Text
- View/download PDF
12. An IMPETUS for New Research Collaborations
- Author
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Kassens, Heidemarie, Lantuit, Hugues, Wegner, C., Baeseman, J., Johansson, M., Kassens, Heidemarie, Lantuit, Hugues, Wegner, C., Baeseman, J., and Johansson, M.
- Abstract
During the IPY, two international hands-on training workshops were held at the Otto-Schmidt Laboratory for Polar and Marine Research at the Arctic and Antarctic Research Institute in St. Petersburg, Russia. These workshops were under the conference heading of IMPETUS, meaning to be the catalyst for new collaborations between young Russian researchers and their international counterparts.IMPETUS 2007 provided insights into the latest techniques and methods used in permafrost research in fields as diverse as permafrost modeling, investigations of mountain ice segregation, bubbling from thermakarst lakes, and submarine permafrost. It brought together experts to provide young investigators with a multidisciplinary and cross-border perspective on permafrost research, a much needed approach in a discipline marked by strong research history yet strongly entangled within national boarders.IMPETUS 2008 focused on the latest techniques and methods used in polar ocean observation in research fields including nutrient and primary production monitoring, data transmission, remote sensing in oceanography and meteorology, sea-ice monitoring, seafloor mapping and Arctic drilling, coastal sediment dynamics, and climate modeling. It brought together experts to provide young researchers with a cross-border perspective in polar ocean research, a needed approach in a field characterized by the presence of many different, partially overlapping disciplines.Both IMPETUS workshops improved existing and built up new collaborations and networks including the whole range from early to senior career scientists. The success of these workshops highlights the need for a sense of belonging among scientists beyond the traditional nationally and disciplinarily established boarders.
- Published
- 2010
13. Association of Polar Early Career Scientists: its origin and history during the International Polar Year 2007-2008
- Author
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Xavier, J., Baeseman, J., Lantuit, Hugues, Xavier, J., Baeseman, J., and Lantuit, Hugues
- Published
- 2010
14. Professional development training for early career polar researchers
- Author
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Thomas, Liz, Baeseman, J., Lantuit, H., Xavier, J.C., Baker, N., Thomas, Liz, Baeseman, J., Lantuit, H., Xavier, J.C., and Baker, N.
- Published
- 2008
15. The First Training Workshop on Permafrost Research Methods: IMPETUS 2007 : OSL-APECS-PYRN Training Workshop; St. Petersburg, Russia, 29 November to 2 December 2007
- Author
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Lantuit, Hugues, Kassens, Heidemarie, Johansson, M., Timokhov, Leonid A., Haltigin, T., Baeseman, J., Volkmann-Lark, Karen, Lantuit, Hugues, Kassens, Heidemarie, Johansson, M., Timokhov, Leonid A., Haltigin, T., Baeseman, J., and Volkmann-Lark, Karen
- Abstract
Fifty young researchers from 14 countries met in St. Petersburg, Russia, to learn about the latest methods used in permafrost research and engineering and to discuss future plans to address climate change issues in permafrost areas. This workshop was an official International Polar Year (IPY) event organized jointly by the Otto Schmidt Laboratory for Polar and Marine Sciences (OSL) in St. Petersburg, the Permafrost Young Researchers Network (PYRN), and the Association of Polar Early Career Scientists (APECS). The workshop provided insights into the latest techniques and methods used in permafrost research in fields as diverse as permafrost modeling, investigations of mountain ice segregation, bubbling from thermokarst lakes, and submarine permafrost detection. It brought together experts to provide young investigators with a multidisciplinary and cross-border perspective on permafrost research, a much needed approach in a discipline marked by strong research history yet strongly entangled within national borders. Presentations and speaker biographies are now available on the conference Web site (http://pyrn.ways.org/activities/pyrn-meetings/2007-saint-petersburg).
- Published
- 2008
- Full Text
- View/download PDF
16. The First Training Workshop on Permafrost Research Methods: IMPETUS 2007: OSL‐APECS‐PYRN Training Workshop; St. Petersburg, Russia, 29 November to 2 December 2007
- Author
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Lantuit, H., primary, Kassens, H., additional, Johansson, M., additional, Timokhov, L., additional, Haltigin, T., additional, Baeseman, J., additional, and Volkmann‐Lark, K., additional
- Published
- 2008
- Full Text
- View/download PDF
17. Professional development training for early career polar researchers
- Author
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Elizabeth Thomas, Baeseman, J., Lantuit, H., Xavier, J., and Baker, N.
18. Arctic science diplomacy maintains Russia co-operation.
- Author
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Berkman PA, Baeseman J, and Shibata A
- Published
- 2022
- Full Text
- View/download PDF
19. Effects of various environmental conditions on the transformation of chlorinated solvents by Methanosarcina thermophila cell exudates.
- Author
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Baeseman JL and Novak PJ
- Subjects
- Biodegradation, Environmental, Soil, Temperature, Environment, Hydrocarbons, Chlorinated chemistry, Solvents chemistry
- Abstract
Several microbiologically produced biomolecules have been shown to degrade chlorinated contaminants found in groundwater systems. It was discovered that the cell-free exudates of the methanogen Methanosarcina thermophila were capable of carbon tetrachloride (CT) and chloroform (CF) degradation. Characterization of the exudates suggested that the active agents were porphorinogen-type molecules, possibly containing zinc. This research was performed to determine if the exudates from M. thermophila could be used for remediation purposes. The cell exudates were found to be capable of degrading CT, CF, tetrachloroethene, trichloroethene, and 1,1,1-trichloroethane. CT degradation was used to gauge exudate activity under a variety of conditions that would be encountered in the environment. The cell exudates were active when incubated in two types of soil matrices and at temperatures ranging from 4 to 23 degrees C. Over a 35-day period approximately 10.2 micromoles of CT were degraded by M. thermophila exudates. To test the hypothesis that the exudates contained either a zinc porphorinogen or a quinone, experiments were performed with zinc 5,10,15,20-tetra (4-pyridyl)-21 H, 23 H-porphine tetrakis, protoporphyrin IX zinc, and juglone. The two zinc porphyrins were capable of mediating CT degradation at rates comparable to those observed with the M. thermophila exudates; however, juglone was only capable of very slow CT transformation. The electron-transfer activity of the M. thermophila cell exudates was therefore more consistent with the activity of porphorinogens rather than quinones. Finally, in two enrichment cultures established from aquifer material and marine sediment, the possibility of excreted agents capable of degrading CT was evident., (Copyright 2001 John Wiley & Sons, Inc.)
- Published
- 2001
- Full Text
- View/download PDF
20. Investigation of cell exudates active in carbon tetrachloride and chloroform degradation.
- Author
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Koons BW, Baeseman JL, and Novak PJ
- Subjects
- Biodegradation, Environmental, Hydrogen-Ion Concentration, Oxygen metabolism, Particle Size, Temperature, Carbon Tetrachloride metabolism, Chloroform metabolism, Methanosarcina metabolism
- Abstract
Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35-65 degrees C, with first-order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were <10 kDa in size, with degradation activity present in both 1-10 kDa and <1 kDa size ranges. Upon purification of the <10 kDa size range of the cell exudates on a C(18) chromatography column, 17 fractions (out of 100) degraded >50% of the added CCl4 in 8 h. These 17 fractions were pooled into three samples based on their elution time from the C(18) column. One of these pooled samples contained elevated levels of cobalt, zinc, and iron, at 2, 3, and 13 times the levels measured in similarly fractionated and pooled samples of medium, respectively. The UV-visible spectrum of this pooled sample had an absorption maximum at 560-580 nm, which is similar to the absorption maxima of heme (approximately 550 and 575 nm). The two other pooled samples contained elevated levels of zinc at 11 and 22 times the concentration measured in similarly fractionated and pooled samples of medium, respectively, and also contained very low levels of nickel, cobalt, and iron. This research suggests that the cell exudates from M. thermophila contain porphorinogen-type molecules capable of dechlorination, possibly excreted corrinoids, hemes, and zinc-containing molecules., (Copyright 2001 John Wiley & Sons, Inc.)
- Published
- 2001
- Full Text
- View/download PDF
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