43 results on '"Bagga, M."'
Search Results
2. Analysis of the non-recombining Y chromosome defines polymorphisms in domestic pig breeds: ancestral bases identified by comparative sequencing
- Author
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Cliffe, K. M., Day, A. E., Bagga, M., Siggens, K., Quilter, C. R., Lowden, S., Finlayson, H. A., Palgrave, C. J., Li, N., Huang, L., Blott, S. C., and Sargent, C. A.
- Published
- 2010
- Full Text
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3. Genetic diversity in European pigs utilizing amplified fragment length polymorphism markers
- Author
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SanCristobal, M., Chevalet, C., Peleman, J., Heuven, H., Brugmans, B., van Schriek, M., Joosten, R., Rattink, A. P., Harlizius, B., Groenen, M. A. M., Amigues, Y., Boscher, M.-Y., Russell, G., Law, A., Davoli, R., Russo, V., Dèsautés, C., Alderson, L., Fimland, E., Bagga, M., Delgado, J. V., Vega-Pla, J. L., Martinez, A. M., Ramos, M., Glodek, P., Meyer, J. N., Gandini, G., Matassino, D., Siggens, K., Laval, G., Archibald, A., Milan, D., Hammond, K., Cardellino, R., Haley, C., and Plastow, G.
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- 2006
4. Genetic diversity within and between European pig breeds using microsatellite markers
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SanCristobal, M., Chevalet, C., Haley, C. S., Joosten, R., Rattink, A. P., Harlizius, B., Groenen, M. A. M., Amigues, Y., Boscher, M.-Y., Russell, G., Law, A., Davoli, R., Russo, V., Désautés, C., Alderson, L., Fimland, E., Bagga, M., Delgado, J. V., Vega-Pla, J. L., Martinez, A. M., Ramos, M., Glodek, P., Meyer, J. N., Gandini, G. C., Matassino, D., Plastow, G. S., Siggens, K. W., Laval, G., Archibald, A. L., Milan, D., Hammond, K., and Cardellino, R.
- Published
- 2006
5. Genetic Diversity Analysis Using Lowly Polymorphic Dominant Markers: The Example of AFLP in Pigs
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Foulley, J.-L., van Schriek, M. G. M., Alderson, L., Amigues, Y., Bagga, M., Boscher, M.-.Y, Brugmans, B., Cardellino, R., Davoli, R., Delgado, J. V., Fimland, E., Gandini, G. C., Glodek, P., Groenen, M. A. M., Hammond, K., Harlizius, B., Heuven, H., Joosten, R., Martinez, A. M., Matassino, D., Meyer, J.-N., Peleman, J., Ramos, A. M., Rattink, A. P., Russo, V., Siggens, K. W., Vega-Pla, J. L., and Ollivier, L.
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- 2006
6. Metalloproteinase production by rabbit articular cartilage: comparison of the effects of interleukin-1α in vitro and in vivo
- Author
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Hembry, R. M., Bagga, M. R., Dingle, J. T., Page Thomas, P., and Reynolds, J. J.
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- 1994
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7. Immunolocalisation studies on six matrix metalloproteinases and their inhibitors, TIMP-1 and TIMP-2, in synovia from patients with osteo- and rheumatoid arthritis
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Hembry, R. M., Bagga, M. R., Reynolds, J. J., and Hamblen, D. L.
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- 1995
8. Utilization of AFLP for genetic distance analysis in pigs
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Heuven, H., Plastow, G., Peleman, J., Siggens, K., Brugmanns, B., and Bagga, M.
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- 2003
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- View/download PDF
9. Genetic diversity in pigs: preliminary results on 58 european breeds and lines
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San Cristobal, Magali, Chevalet, Claude, Haley, C.S., Russell, G., Plastow, G., Siggens, K., Bagga, M., Groenen, M.A.M., Amigues, Yves, Hammond, K., Laval, Guillaume, Boscher, Marie Yvonne, Milan, Denis, Law, A., Fimland, E., Davoli, R., Russo, V., Gandini, G., Archibald, Alan Langskill, Delgado, J.V., Ramos, M., Desautes, C., Alderson, L., Glodek, P., Meyer, J.N., Foulley, Jean Louis, Ollivier, Louis, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), Institut National de la Recherche Agronomique (INRA), Station de Génétique Quantitative et Appliquée (SGQA), and ProdInra, Migration
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[SDV] Life Sciences [q-bio] ,pig ,molecular marker ,[SDV]Life Sciences [q-bio] ,genetic diversity ,europe ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2002
10. Utilización de los AFLP(C) para el análisis de distancias genéticas en cerdos
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Plastow, G., Siggens, K., Bagga, M., Brugmans, B., Heuven, H., Peleman, J., Plastow, G., Siggens, K., Bagga, M., Brugmans, B., Heuven, H., and Peleman, J.
- Abstract
Los AFLPs han sido utilizados con éxito como herramienta para generar un gran número (más de cien) de marcadores de ADN en 2435 individuos del proyecto Europeo sobre Biodiversidad Porcina. Esta información fue utilizada para realizar un análisis inicial de distancias genéticas que permitió agrupar líneas y razas según sugería la información descriptiva.
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- 2003
11. Reply: Array comparative genomic hybridization for the detection of submicroscopic copy number variations of the X chromosome in women with premature ovarian failure
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Quilter, C. R., primary, Karcanias, A. C., additional, Bagga, M. R., additional, Duncan, S., additional, Murray, A., additional, Conway, G. S., additional, Sargent, C. A., additional, and Affara, N. A., additional
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- 2010
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12. Analysis of X chromosome genomic DNA sequence copy number variation associated with premature ovarian failure (POF)
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Quilter, C. R., primary, Karcanias, A. C., additional, Bagga, M. R., additional, Duncan, S., additional, Murray, A., additional, Conway, G. S., additional, Sargent, C. A., additional, and Affara, N. A., additional
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- 2010
- Full Text
- View/download PDF
13. Metalloproteinase production by rabbit articular cartilage: comparison of the effects of interleukin-1 alpha in vitro and in vivo.
- Author
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Hembry, R., Bagga, M., Dingle, J., Page Thomas, P., Reynolds, J., Hembry, R M, Bagga, M R, Dingle, J T, Thomas, P P, and Reynolds, J J
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ANIMAL experimentation ,ARTICULAR cartilage ,CELL culture ,COMPARATIVE studies ,GLYCOPROTEINS ,GROWTH factors ,IMMUNOHISTOCHEMISTRY ,INTERLEUKIN-1 ,RESEARCH methodology ,MEDICAL cooperation ,PROTEINS ,PROTEOLYTIC enzymes ,RABBITS ,RECOMBINANT proteins ,RESEARCH ,SWINE ,EVALUATION research ,DEXAMETHASONE ,PHARMACODYNAMICS - Abstract
To assess the effects of interleukin-1 on intact To assess the effects of interleukin-1 on intact articular cartilage in vitro, explants from young and adult rabbits were cultured with interleukin-1 and the distributions of the matrix metalloproteinases and tissue inhibitor of metalloproteinases (TIMP-1) were investigated by indirect immunofluorescence microscopy. One to 2-week-old cartilage chondrocytes synthesized collagenase in response to pure or crude interleukin-1 (monocyte conditioned medium), with subarticular cells most responsive. Collagenase synthesis was not stimulated in adult articular chondrocytes when explants were treated with either pure or crude interleukin-1. Stromelysin, gelatinase and TIMP-1 could not be demonstrated within any zone of the cartilage, indicating that their synthesis was not stimulated by either pure or crude interleukin-1. The addition of fibroblast growth factors, either alone or in combination with interleukin-1, did not modify these responses. These results contrast markedly with observations on cultured chondrocyte monolayers, where interleukin-1 treatment induces near co-ordinate expression of metalloproteinases. To assess the effects of interleukin-1 in vivo, it was injected into adult rabbit knee joint spaces and the articular cartilage subsequently analysed for evidence of altered metalloproteinase production by immunocytochemistry. No significant increase in metalloproteinase or TIMP-1 synthesis by chondrocytes was detected, although the cartilage matrix showed a marked loss of toluidine blue metachromasia. We conclude that metalloproteinases are not involved in the rapid loss of proteoglycan from cartilage matrix in these situations. [ABSTRACT FROM AUTHOR]
- Published
- 1994
- Full Text
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14. Reciprocal chromosome painting shows that genomic rearrangement between rat and mouse proceeds ten times faster than between humans and cats
- Author
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Stanyon, R., primary, Yang, F., additional, Cavagna, P., additional, O’Brien, P.C.M., additional, Bagga, M., additional, Ferguson-Smith, M.A., additional, and Wienberg, J., additional
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- 1999
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15. Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome
- Author
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Griffin, D.K., primary, Haberman, F., additional, Masabanda, J., additional, O’Brien, P., additional, Bagga, M., additional, Sazanov, A., additional, Smith, J., additional, Burt, D.W., additional, Ferguson-Smith, M., additional, and Wienberg, J., additional
- Published
- 1999
- Full Text
- View/download PDF
16. Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome.
- Author
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Griffin, D.K., Haberman, F., Masabanda, C J., O'brien, P., Bagga, M., Sazanov, A., Smith, J., Burt, D.W., Ferguson-Smith, M., and Wienberg, J.
- Subjects
ANIMAL genome mapping ,CHROMOSOMES ,KARYOTYPES ,DNA ,CHICKENS ,MICRODISSECTION ,FLOW cytometry - Abstract
Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes. Copyright © 2000 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
17. Reciprocal chromosome painting shows that genomic rearrangement between rat and mouse proceeds ten times faster than between humans and cats.
- Author
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Stanyon, R., Yang, F., Cavagna, P., O'brien, P. C. M., Bagga, M., Ferguson-smith, M. A., and Wienberg, J.
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GENE mapping ,HOMOLOGY (Biology) ,CHROMOSOME banding ,FLUORESCENCE in situ hybridization ,KARYOTYPES ,MAMMALS ,GENOMICS - Abstract
Reciprocal chromosome painting between mouse and rat using complete chromosome probe sets of both species permitted us to assign the chromosomal homology between these rodents. The comparative gene mapping data and chromosome painting have a better than 90% correspondence. The reciprocal painting results graphically show that mouse and rat have strikingly different karyotypes. At least 14 translocations have occurred in the 10–20 million years of evolution that separates these two species. The evolutionary rate of chromosome translocations between these two rodents appears to be up to 10 times greater than that found between humans and cats, or between humans and chimpanzees, where over the last 5–6 million years just one translocation has occurred. Outgroup comparison shows that the mouse genome has incorporated at least three times the amount of interchromosomal rearrangements compared to the rat genome. The utility of chromosome painting was also illustrated by the assignment of two new chromosome homologies between rat and mouse unsuspected by gene mapping: between mouse 11 and rat 20 and between mouse 17 and rat 6. We conclude that reciprocal chromosome painting is a powerful method, which can be used with confidence to chart the genome and predict the chromosome location of genes. Reciprocal painting combined with gene mapping data will allow the construction of large-scale comparative chromosome maps between placental mammals and perhaps other animals. [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
18. Variations in the serum concentration and urine excretion of alpha 2HS-glycoprotein, a bone-related protein, in normal individuals and in patients with osteogenesis imperfecta.
- Author
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Dickson, I R, Bagga, M, and Paterson, C R
- Abstract
The concentration of alpha 2HS-glycoprotein was measured in the serum and urine of normal individuals and of patients with osteogenesis imperfecta. The serum concentration of alpha 2HS-glycoprotein was higher in normal children than in adults. In women values showed a progressive age-related decrease, from 632 mg/l at 21-30 years to 573 mg/l at 51-60 years. In men there was no such age-related variation, and values were higher than in women of comparable age; the mean value for men aged 20-60 years was 648 mg/l. Of 48 patients with osteogenesis imperfecta, 11 had an abnormally high concentration of alpha 2HS-glycoprotein in serum; the cause of this is not clear. In urine of 24 normal individuals the mean value of the ratio albumin: alpha 2HS-glycoprotein was 20 +/- 3; in serum the corresponding ratio was 70. Urine excretion of alpha 2HS-glycoprotein was lowest in female children (132 +/- 29 micrograms/24 h) and highest in male adults (592 +/- 91 micrograms/24 h); values in patients with osteogenesis imperfecta did not differ from normal. [ABSTRACT FROM AUTHOR]
- Published
- 1983
19. Iron and palladium complexes from azobenzenes and o-diaminobenzene.
- Author
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Bagga, M. M., Flannigan, W. T., Knox, G. R., and Pauson, P. L.
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- 1969
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20. Iron carbonyl complexes from Schiff bases.
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Bagga, M. M., Flannigan, W. T., Knox, G. R., Pauson, P. L., Preston, F. J., and Reed, R. I.
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- 1968
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21. Effect of glucose-mediated LDL oxidation on the P388D1 macrophage-like cell line
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Millican, S. A., Bagga, M., Eddy, R., Mitchinson, M. J., and Hunt, J. V.
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- 1997
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22. Microneedles in transdermal drug delivery
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Bagga, M., Ghosh, A., and MANOJ KUMAR DEKA
23. ChemInform Abstract: A Novel Reaction of Formaldehyde with Some Diamines.
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BAGGA, M. M., primary, EVERATT, B., additional, and HINTON, I. G., additional
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- 1987
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24. Iron complexes from diaryldiazomethanes
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Bagga, M. M., primary, Baikie, P. E., additional, Mills, O. S., additional, and Pauson, P. L., additional
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- 1967
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25. ChemInform Abstract: CARBEN-EINSCHIEBUNG IN EINE KOHLENSTOFF-EISEN-BINDUNG
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BAGGA, M. M., primary, FERGUSON, G., additional, JEFFREYS, J. A. D., additional, MANSELL, C. M., additional, PAUSON, P. L., additional, ROBERTSON, I. C., additional, and SIME, J. G., additional
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- 1970
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26. Carbene insertion into a carbon–iron bond
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Bagga, M. M., primary, Ferguson, G., additional, Jeffreys, J. A. D., additional, Mansell, C. M., additional, Pauson, P. L., additional, Robertson, I. C., additional, and Sime, J. G., additional
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- 1970
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27. Complexes of azobenzenes and Schiff bases
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Bagga, M. M., primary, Pauson, P. L., additional, Preston, F. J., additional, and Reed, R. I., additional
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- 1965
- Full Text
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28. Carbene insertion into a carbon–iron bond.
- Author
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Bagga, M. M., Ferguson, G., Jeffreys, J. A. D., Mansell, C. M., Pauson, P. L., Robertson, I. C., and Sime, J. G.
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- 1970
- Full Text
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29. Genetic diversity in European pigs utilizing amplified fragment length polymorphism markers
- Author
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Guillaume Laval, P. Glodek, R. Cardellino, Alan Archibald, A.P. Rattink, Graham Plastow, Martien A. M. Groenen, Vincenzo Russo, M. Bagga, Henri C M Heuven, Amparo Martínez Martínez, J. Peleman, B. Brugmans, Juan Vicente Delgado, Chris Haley, R. Joosten, K. Hammond, L. Alderson, Maria Ramos, E. Fimland, Barbara Harlizius, J. N. Meyer, K. Siggens, Andy Law, Donato Matassino, Roberta Davoli, George C. Russell, Claude Chevalet, Gustavo Gandini, Y. Amigues, Magali SanCristobal, C. Désautés, David J. Milan, Jose Luis Vega-Pla, M.-Y. Boscher, M. G. M. van Schriek, ProdInra, Migration, San Cristobal M., Chevalet C., Peleman J., Heuven H., Brugmans B., Van Schriek M., Joosten R., Rattink A.P., Harlizius B., Groenen M.A.M., Amigues Y., Boscher M.-Y, Russel G., Law A., Davoli R., Russo V., Desautes C., Alderson L., Fimland E., Bagga M., Delgado J.V., Vega-Pla J., Martinez A.M., Ramos M., Glodeck P., Meyer J.N., Gandini G., Matassino D., Siggens K., Laval G., Archibald A., Milan D., Hammond K., Cardellino R., Haley C., Plastow G., Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), and Institut National de la Recherche Agronomique (INRA)
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pig ,Genetic Markers ,0106 biological sciences ,Heterozygote ,Genotype ,Swine ,[SDV]Life Sciences [q-bio] ,AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS ,Breeding ,Biology ,010603 evolutionary biology ,01 natural sciences ,Loss of heterozygosity ,03 medical and health sciences ,Genetics ,Animals ,Allele ,Alleles ,Phylogeny ,030304 developmental biology ,0303 health sciences ,Genetic diversity ,genetic distance ,Polymorphism, Genetic ,conservation ,food and beverages ,genetic diversity ,General Medicine ,[SDV] Life Sciences [q-bio] ,Europe ,Genetic distance ,Genetic marker ,Microsatellite ,Animal Science and Zoology ,Amplified fragment length polymorphism ,aflp marker ,Microsatellite Repeats - Abstract
The use of DNA markers to evaluate genetic diversity is an important component of the management of animal genetic resources. The Food and Agriculture Organisation of the United Nations (FAO) has published a list of recommended microsatellite markers for such studies; however, other markers are potential alternatives. This paper describes results obtained with a set of amplified fragment length polymorphism (AFLP) markers as part of a genetic diversity study of European pig breeds that also utilized microsatellite markers. Data from 148 AFLP markers genotyped across samples from 58 European and one Chinese breed were analysed. The results were compared with previous analyses of data from 50 microsatellite markers genotyped on the same animals. The AFLP markers had an average within-breed heterozygosity of 0.124 but there was wide variation, with individual markers being monomorphic in 3-98% of the populations. The biallelic and dominant nature of AFLP markers creates a challenge for their use in genetic diversity studies as each individual marker contains limited information and AFLPs only provide indirect estimates of the allelic frequencies that are needed to estimate genetic distances. Nonetheless, AFLP marker-based characterization of genetic distances was consistent with expectations based on breed and regional distributions and produced a similar pattern to that obtained with microsatellites. Thus, data from AFLP markers can be combined with microsatellite data for measuring genetic diversity.
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- 2006
30. Genetic diversity within and between European pig breeds using microsatellite markers
- Author
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K. Siggens, Claude Chevalet, Gustavo Gandini, R. Joosten, Magali SanCristobal, Alan Archibald, George C. Russell, M. Bagga, David J. Milan, Roberta Davoli, L. Alderson, Jose Luis Vega-Pla, Donato Matassino, E. Fimland, A.P. Rattink, Martien A. M. Groenen, P. Glodek, Juan Vicente Delgado, Graham Plastow, Chris Haley, K. Hammond, Andy Law, C. Désautés, Amparo Martínez Martínez, Guillaume Laval, Maria Ramos, J. N. Meyer, R. Cardellino, Y. Amigues, Barbara Harlizius, Vincenzo Russo, M.-Y. Boscher, San Cristobal M., Chevalet C., Haley C.S., Joosten R., Rattink A.P., Harlizius M., Groenen A.M., Amigues Y., Boscher M.-Y., Russell G., Law A., Davoli R., Russo V., Desautes C., Alderson L., Fimland E., Bagga M., Delgado J.V., Vega-Pla J.L., Martinez A.M., Ramos M., Glodek P., Meyer J.N., Gandini G.C., Matassino D., Plastow G.S., Siggens K.W., Laval G., A.L. Archibald, Milan D., Hammond K., Cardellino R., Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration
- Subjects
Animal breeding ,Swine ,[SDV]Life Sciences [q-bio] ,statistische analyse ,Population genetics ,CLUSTERING ,genetische merkers ,rasverschillen ,Breeding ,microsatellites ,genetische diversiteit ,Gene Frequency ,humans ,biodiversity ,Genetics ,0303 health sciences ,genetic distance ,pig breeds ,conservation ,pigs ,dierveredeling ,04 agricultural and veterinary sciences ,General Medicine ,animal breeding ,genetic diversity ,wilde varkens ,populatiegenetica ,varkens ,Breed ,[SDV] Life Sciences [q-bio] ,Europe ,allelen ,GENETIC DISTANCE ,alleles ,loci ,Microsatellite ,Biodiversity ,Clustering ,Genetic distance ,Microsatellites ,Pig ,Settore AGR/19 - Zootecnica Speciale ,microsatellite ,Genotype ,microsatellieten ,MICROSATELLITES ,Zoology ,wild pigs ,Biology ,Animal Breeding and Genomics ,heterozygotie ,03 medical and health sciences ,statistical analysis ,varkensrassen ,Genetic variation ,evolution ,Animals ,heterozygosity ,Fokkerij en Genomica ,distance ,030304 developmental biology ,Genetic diversity ,PIG ,0402 animal and dairy science ,biodiversiteit ,Genetic Variation ,population genetics ,genetische afstand ,populations ,040201 dairy & animal science ,breed differences ,Genetic marker ,WIAS ,genetic markers ,Animal Science and Zoology ,BIODIVERSITY ,Microsatellite Repeats ,conservering - Abstract
An important prerequisite for a conservation programme is a comprehensive description of genetic diversity. The aim of this study was to use anonymous genetic markers to assess the between- and the within-population components of genetic diversity for European pig breeds at the scale of the whole continent using microsatellites. Fifty-eight European pig breeds and lines were analysed including local breeds, national varieties of international breeds and commercial lines. A sample of the Chinese Meishan breed was also included. Eleven additional breeds from a previous project were added for some analyses. Approximately 50 individuals per breed were genotyped for a maximum of 50 microsatellite loci. Substantial within-breed variability was observed, with the average expected heterozygosity and observed number of alleles per locus being 0.56 [range 0.43-0.68] and 4.5 respectively. Genotypic frequencies departed from Hardy-Weinberg expectations (P < 0.01) in 15 European populations, with an excess of homozygotes in 12 of them. The European breeds were on average genetically very distinct, with a Wright F(ST) index value of 0.21. The Neighbour-Joining tree drawn from the Reynolds distances among the breeds showed that the national varieties of major breeds and the commercial lines were mostly clustered around their breeds of reference (Duroc, Hampshire, Landrace, Large White and Pietrain). In contrast, local breeds, with the exception of the Iberian breeds, exhibited a star-like topology. The results are discussed in the light of various forces, which may have driven the recent evolution of European pig breeds. This study has consequences for the interpretation of biodiversity results and will be of importance for future conservation programmes.
- Published
- 2006
31. An assessment of European pig diversity using molecular markers: Partitioning of diversity among breeds
- Author
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Alan Archibald, Jose Luis Vega-Pla, Keith Hammond, Meena Bagga, Vincenzo Russo, Andy Law, Céline Désautés, Erling Fimland, Marie-Yvonne Boscher, L. Ollivier, A.P. Rattink, Barbara Harlizius, Graham Plastow, Guillaume Laval, Amparo Martínez Martínez, K. Siggens, Chris Haley, Roberta Davoli, Y. Amigues, Magali San Cristobal, Denis Milan, Claude Chevalet, Gustavo Gandini, Ricardo Cardellino, R. Joosten, Lawrence Alderson, A. M. Ramos, J. V. Delgado, Donato Matassino, Johann-Nikolaus Meyer, Jean-Louis Foulley, Geraldine Russell, Martien A. M. Groenen, Peter Glodek, ProdInra, Migration, Ollivier L., Alderson L., Gandini G.C, Foulley J.-L., Haley C.S., Joosten R., Rattink A.P., Harlizius M., Groenen A.M., Amigues Y., Boscher M.-Y., Russell G., Law A., Davoli R., Russo V., Matassino D., Desautes C., Fimland E., Bagga M., Delgado J.V., Vega-Pla J.L., Martinez A.M., Ramos M., Glodek P., Meyer J.N., Plastow G.S., Siggens K.W., A.L. Archibald, Milan D., San Cristobal M., Laval G., Hammond K., Cardellino R., Chevalet C., Station de Génétique Quantitative et Appliquée (SGQA), Institut National de la Recherche Agronomique (INRA), Laboratoire d'Analyse Génétique pour les Espèces Animales (LABOGENA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
- Subjects
Conservation genetics ,pig ,Animal breeding ,[SDV]Life Sciences [q-bio] ,Biodiversity ,purposes ,genetische merkers ,rasverschillen ,cryopreservation ,microsatellites ,amplified fragment length polymorphism ,genetische diversiteit ,cryopreservering ,PIGS ,ComputingMilieux_MISCELLANEOUS ,biodiversity ,2. Zero hunger ,Genetics ,0303 health sciences ,extinction ,pig breeds ,conservation ,subdivided populations ,pigs ,dierveredeling ,04 agricultural and veterinary sciences ,animal breeding ,genetic diversity ,kruising ,varkens ,Breed ,crossbreds ,[SDV] Life Sciences [q-bio] ,uitsterven ,moleculaire genetica ,Microsatellite ,genetic marker ,management ,Settore AGR/19 - Zootecnica Speciale ,microsatellieten ,CONSERVATION ,Zoology ,Biology ,Animal Breeding and Genomics ,size ,aflp ,03 medical and health sciences ,varkensrassen ,Fokkerij en Genomica ,distance ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,livestock breeds ,Genetic diversity ,0402 animal and dairy science ,biodiversiteit ,biodiversity, conservation, genetic marker, pig ,040201 dairy & animal science ,breed differences ,cattle breeds ,GENETIC MARKERS ,conservation genetics ,molecular genetics ,genetic markers ,WIAS ,Amplified fragment length polymorphism ,BIODIVERSITY ,Purebred ,human activities ,conservering - Abstract
Genetic diversity within and between breeds (and lines) of pigs was investigated. The sample comprised 68 European domestic breeds (and lines), including 29 local breeds, 18 varieties of major international breeds, namely Duroc, Hampshire, Landrace, Large White and Pietrain, and 21 commercial lines either purebred or synthetic, to which the Chinese Meishan and a sample of European wild pig were added. On average 46 animals per breed were sampled (range 12–68). The genetic markers were microsatellites (50 loci) and AFLP (amplified fragment length polymorphism, 148 loci). The analysis of diversity showed that the local breeds accounted for 56% of the total European between-breed microsatellite diversity, and slightly less for AFLP, followed by commercial lines and international breeds. Conversely, the group of international breeds contributed most to within-breed diversity, followed by commercial lines and local breeds. Individual breed contributions to the overall European between- and within-breed diversity were estimated. The range in between-breed diversity contributions among the 68 breeds was 0.04–3.94% for microsatellites and 0.24–2.94% for AFLP. The within-breed diversity contributions varied very little for both types of markers, but microsatellite contributions were negatively correlated with the between-breed contributions, so care is needed in balancing the two types of contribution when making conservation decisions. By taking into account the risks of extinction of the 29 local breeds, a cryopreservation potential (priority) was estimated for each of them.
- Published
- 2005
32. Chemical Profile of the Pits Oil from the Tunisian 'Alig' Cultivar of Phoenix dactylifera L.: In Vivo Wound Healing Potential Evaluation of a Cream Formulated from the Extracted Oil and Insights from Molecular Docking and SAR Analysis.
- Author
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Chhoud R, Said Bagga M, Ali Lassoued M, Jlizi S, Nabili A, Sfar S, Ben Jannet H, and Majdoub H
- Subjects
- Rats, Animals, Molecular Docking Simulation, Spectroscopy, Fourier Transform Infrared, Plant Oils chemistry, Wound Healing, Fatty Acids analysis, Oleic Acid, Phoeniceae chemistry
- Abstract
Since ancient times the oil from date palm pits (Phoenix dactylifera L.) has been used to heal wounds. In order to prove this traditional usage of the pits, this oil was extracted from the pits of the Tunisian cultivar 'Alig' and its physico-chemical properties and the chemical composition were evaluated. The fatty acid profile, evidenced by GC, allowed to classify this oil as an oleic-myristic acid oil with a clear abundance of oleic acid (53.66 %).
1 H and13 C-NMR as well as FT-IR analyses confirmed the presence of fatty acids in triglyceride forms. Furthermore, in vivo wound healing activity of a cream formulated from the extracted oil was performed, for the first time, using a rat model and was compared to placebo cream and a commercial formulation, MEBO®. This study showed that the test cream promoted the healing of pressure ulcers better than the placebo cream and the MEBO® ointment. The results showed that this vegetable oil is able to improve the healing of infected wounds in rats, thus supporting its traditional use. The contribution of the main oleic, linoleic and myristic acids that can be derived from enzymatic hydrolysis to the healing activity of the whole pits oil was predicted by in silico study and the calculated pharmacokinetics parameters., (© 2022 Wiley-VHCA AG, Zurich, Switzerland.)- Published
- 2023
- Full Text
- View/download PDF
33. Just-in-Time Interdisciplinary Education for Hospice Care of Ventricular-Assist Device Patients.
- Author
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Kossman DA, Wolfrom SD, Haines S, Bagga M, Denno M, Ljubic L, and O'Connor N
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Health Personnel education, Heart Failure surgery, Heart-Assist Devices, Hospice Care methods, Hospice Care standards, Interdisciplinary Studies, Practice Guidelines as Topic
- Abstract
Introduction: Ventricular-assist devices (VADs) offer end-stage heart failure patients a chance to live longer and better. VAD patient numbers are growing, as is the need for their hospice care, whether they die from cardiac or noncardiac causes. But many hospices do not accept VAD patients because of unfamiliarity with the technology. In 2017, there were no hospice agencies in our area accepting VAD patients. Case Description: Mr. S was a 61-year-old man with a VAD implanted in 2011. In 2016, he was diagnosed with metastatic colorectal adenocarcinoma, underwent surgical resection, and suffered many postoperative complications. In January 2017, he was hospitalized for a driveline infection and bacteremia. During that stay, Mr. S opted for comfort care with the goal of returning home. The only chance of Mr. S's going home rested on our hospice agency's accepting him. In less than two weeks, our hospice staff partnered with the patient's VAD team to develop the competencies to care for Mr. S. He was stabilized at our inpatient unit and then spent several weeks at home before symptoms required return to inpatient care. When Mr. S became unresponsive, his family chose to inactivate the VAD; Mr. S's death followed quickly and peacefully. Our agency now routinely provides hospice care for VAD patients. Discussion: It is increasingly important that hospice agencies accept VAD patients seeking hospice care. By drawing on educational resources available from VAD patients' acute-care hospital-based VAD teams, interdisciplinary education of hospice staff can be accomplished quickly and effectively.
- Published
- 2019
- Full Text
- View/download PDF
34. Training Residents in the Use of Telepsychiatry: Review of the Literature and a Proposed Elective.
- Author
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Saeed SA, Johnson TL, Bagga M, and Glass O
- Subjects
- Clinical Competence standards, Humans, Curriculum, Education, Medical, Graduate standards, Internship and Residency standards, Psychiatry education, Telemedicine methods
- Abstract
Many regions in the United States are deficient in mental health services, especially those in rural areas. As a result of these deficiencies, many patients in need of psychiatric services are often left untreated. Although it is clear that telepsychiatry has great potential in improving patient access to mental health care in areas where psychiatric services are deficient, the lack of familiarity with the technology and inadequate training are current barriers to expanding the use of telepsychiatry. A review of telepsychiatry, its clinical applications, and evidence-based literature regarding competencies in graduate medical education related to telepsychiatry are provided. An approach to implementing telepsychiatry into a curriculum is suggested. We also propose an elective clinical experience with resources for didactics or independent study that will enable residents to develop a knowledge base and competence in the practice of telepsychiatry.
- Published
- 2017
- Full Text
- View/download PDF
35. ColonyArea: an ImageJ plugin to automatically quantify colony formation in clonogenic assays.
- Author
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Guzmán C, Bagga M, Kaur A, Westermarck J, and Abankwa D
- Subjects
- Cell Line, Tumor, Humans, Staurosporine analogs & derivatives, Staurosporine pharmacology, Tumor Cells, Cultured, Colony-Forming Units Assay methods
- Abstract
The clonogenic or colony formation assay is a widely used method to study the number and size of cancer cell colonies that remain after irradiation or cytotoxic agent administration and serves as a measure for the anti-proliferative effect of these treatments. Alternatively, this assay is used to quantitate the transforming potential of cancer associated genes and chemical agents. Therefore, there is a need for a simplified and standardized analysis of colony formation assays for both routine laboratory use and for parallelized automated analysis. Here we describe the freely available ImageJ-plugin "ColonyArea", which is optimized for rapid and quantitative analysis of focus formation assays conducted in 6- to 24-well dishes. ColonyArea processes image data of multi-well dishes, by separating, concentrically cropping and background correcting well images individually, before colony formation is quantitated. Instead of counting the number of colonies, ColonyArea determines the percentage of area covered by crystal violet stained cell colonies, also taking the intensity of the staining and therefore cell density into account. We demonstrate that these parameters alone or in combination allow for robust quantification of IC50 values of the cytotoxic effect of two staurosporines, UCN-01 and staurosporine (STS) on human glioblastoma cells (T98G). The relation between the potencies of the two compounds compared very well with that obtained from an absorbance based method to quantify colony growth and to published data. The ColonyArea ImageJ plugin provides a simple and efficient analysis routine to quantitate assay data of one of the most commonly used cellular assays. The bundle is freely available for download as supporting information. We expect that ColonyArea will be of broad utility for cancer biologists, as well as clinical radiation scientists.
- Published
- 2014
- Full Text
- View/download PDF
36. Gene structure and expression of serotonin receptor HTR2C in hypothalamic samples from infanticidal and control sows.
- Author
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Quilter CR, Bagga M, Moinie A, Junaid F, and Sargent CA
- Subjects
- Animals, Genetic Loci, Molecular Sequence Data, Polymorphism, Genetic, RNA Editing, Receptor, Serotonin, 5-HT2C metabolism, Swine, Behavior, Animal physiology, Hypothalamus metabolism, Maternal Behavior physiology, Receptor, Serotonin, 5-HT2C genetics
- Abstract
Background: The serotonin pathways have been implicated in behavioural phenotypes in a number of species, including human, rat, mouse, dog and chicken. Components of the pathways, including the receptors, are major targets for drugs used to treat a variety of physiological and psychiatric conditions in humans. In our previous studies we have identified genetic loci potentially contributing to maternal infanticide in pigs, which includes a locus on the porcine X chromosome long arm. The serotonin receptor HTR2C maps to this region, and is therefore an attractive candidate for further study based on its function and its position in the genome., Results: In this paper we describe the structure of the major transcripts produced from the porcine HTR2C locus using cDNA prepared from porcine hypothalamic and pooled total brain samples. We have confirmed conservation of sites altered by RNA editing in other mammalian species, and identified polymorphisms in the gene sequence. Finally, we have analysed expression and editing of HTR2C in hypothalamus samples from infanticidal and control animals., Conclusions: The results confirm that although the expression of the long transcriptional variant of HTR2C is raised in infanticidal animals, the overall patterns of editing in the hypothalamus are similar between the two states.Sequences associated with the cDNA and genomic structures of HTR2C reported in this paper are deposited in GenBank under accession numbers FR720593, FR720594 and FR744452.
- Published
- 2012
- Full Text
- View/download PDF
37. A systematic review of different type of tuberculosis.
- Author
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Kumar SV, Deka MK, Bagga M, Kala MS, and Gauthaman K
- Subjects
- Humans, Tuberculosis diagnosis, Tuberculosis epidemiology, Tuberculosis prevention & control, Tuberculosis classification
- Abstract
Tuberculosis (TB) is still a leading cause of death worldwide. Almost a third of the world's population is infected with TB bacilli, and each year approximately 8 million people develop active tuberculosis and 2 million die as a result. However, there are few studies of long-term TB treatment outcomes from Directly Observed Therapy, Short-course (DOTS) programs in high-burden settings and particularly settings of high drug resistance. This study is a systematic review to evidence the incidence and prevalence of latent TB infection (LTBI) and disease and to evaluate the impact of various preventive strategies that have been attempted. To identify relevant studies, we searched electronic databases and journals, and contacted experts in the field. This review demonstrates that, various types of tuberculosis have different imaging findings, and typical computed tomography (CT) and magnetic resonance (MG) findings can suggest the diagnosis. Available evidence reinforces the need to design and implement simple, effective, and affordable tuberculosis infection-control programs in health-care facilities in our countries. With the revision of all the data's, we are able to conclude that the controlling of tuberculosis by human beings is yet not achieved. So, there is an urgency to develop awareness amongst the individuals and also a new drugs regimen for the proper treatment of tuberculosis.
- Published
- 2010
38. Glucose-modified low density lipoprotein enhances human monocyte chemotaxis.
- Author
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Millican SA, Schultz D, Bagga M, Coussons PJ, Müller K, and Hunt JV
- Subjects
- Chelating Agents pharmacology, Chemotaxis, Leukocyte drug effects, Enzyme Inhibitors pharmacology, Glycosylation, Guanidines pharmacology, Humans, Monocytes drug effects, Oxidation-Reduction, Pentetic Acid pharmacology, Chemotaxis, Leukocyte physiology, Glucose metabolism, Lipoproteins, LDL physiology, Monocytes physiology
- Abstract
In diabetes mellitus the progression of atherosclerosis is accelerated. The interaction of glucose with atherogenic lipoproteins may be relevant to the mechanisms responsible for this vascular damage. The aim of this study was to examine the effect of glucose-modified low density lipoprotein (LDL) on human monocyte chemotaxis and to investigate the roles of oxidation and glycation in the generation of chemotactic LDL. Cu(II)-mediated LDL oxidation was potentiated by glucose in a dose-dependent manner and increased its chemotactic activity. Incubation with glucose alone, under conditions where very little oxidation was observed, also increased the chemotactic property of LDL. Neither diethylenetriamine pentaacetic acid (DETAPAC) nor aminoguanidine, which both inhibited LDL oxidation, completely inhibited the chemotactic activity of glycated oxidised LDL. The results suggest that both oxidation and glycation contribute to increased chemotactic activity.
- Published
- 1998
- Full Text
- View/download PDF
39. Stromelysin, gelatinase A and TIMP-1 in prosthetic interface tissue: a role for macrophages in tissue remodelling.
- Author
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Hembry RM, Bagga MR, Reynolds JJ, and Hamblen DL
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Hip Joint pathology, Humans, Immunohistochemistry, Knee Joint pathology, Macrophages metabolism, Male, Matrix Metalloproteinase 2, Middle Aged, Tissue Inhibitor of Metalloproteinases, Gelatinases analysis, Glycoproteins analysis, Hip Joint metabolism, Joint Prosthesis, Knee Joint metabolism, Macrophages pathology, Matrix Metalloproteinase 3 analysis, Metalloendopeptidases analysis
- Abstract
Aseptic loosening of prosthetic components is the most important long-term complication of total joint replacement. To investigate the underlying destructive mechanisms, periprosthetic tissues from both well-fixed and loosened sites from six patients, undergoing surgery for aseptic loosening of knee or hip prostheses, were analysed in detail by immunohistochemical methods for the presence of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 (TIMP-1). The tissues contained small numbers of cells positive for either collagenase, stromelysin, gelatinase A or TIMP-1; these were randomly distributed, neither specifically next to the bone interface nor to wear particles, and the number of positive cells did not correlate with macroscopic observations at operation. Gelatinase A was co-localized in cells with prolyl-4-hydroxylase, an enzyme involved in collagen synthesis. The predominant cell type in these tissues was shown to be the macrophage by the use of cell marker antibodies. Dual localization was not technically possible but the results strongly suggest that monocyte/macrophages were the primary source of gelatinase A and TIMP-1. Stromelysin was immunolocalized on connective tissue matrix in four patients, and gelatinase A in one patient, and were also observed in tissues in which there was no evidence of cellular synthesis of these enzymes. This suggests that secretion had taken place previously, resulting in enzyme bound to matrix for some time. Taken together, these data indicate that localized focal connective tissue remodelling occurs in periprosthetic tissues from both well fixed and loosened sites.
- Published
- 1995
- Full Text
- View/download PDF
40. Rabbit models of arthritis: immunolocalization of matrix metalloproteinases and tissue inhibitor of metalloproteinase in synovium and cartilage.
- Author
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Hembry RM, Bagga MR, Murphy G, Henderson B, and Reynolds JJ
- Subjects
- Animals, Arthritis, Experimental pathology, Cartilage pathology, Fluorescent Antibody Technique, Hyaluronic Acid, Metalloendopeptidases antagonists & inhibitors, Ovalbumin, Polylysine, Rabbits, Synovitis pathology, Tissue Inhibitor of Metalloproteinase-2, Arthritis, Experimental enzymology, Cartilage enzymology, Metalloendopeptidases metabolism, Neoplasm Proteins metabolism, Synovitis enzymology
- Abstract
The distribution of the matrix metalloproteinases, collagenase, stromelysin, gelatinases A and B, and the tissue inhibitor of metalloproteinases in cartilage and synovium removed from rabbits up to 27 days after induction of two models of arthritis was investigated by immunolocalization. Following intra-articular injection of poly-D-lysine/hyaluronic acid coacervate, collagenase and stromelysin were found bound to cartilage matrix, but there was little increase in chondrocyte synthesis of these enzymes. The synovium underwent a complex wound healing response involving invagination and encapsulation of the coacervate and inflammatory cell debris, during which all four metalloproteinases and tissue inhibitor of metalloproteinase could be immunolocalized. The second model, intra-articular injection of ovalbumin into sensitized rabbits, caused considerable chondrocyte necrosis; collagenase was found bound to cartilage matrix on day 13, although again there was little evidence of synthesis by chondrocytes. Inflammatory cell infiltration of meniscoid synovia took place initially, followed by fibrosis involving macrophagelike cells secreting gelatinase A. In both models there was rapid loss of glycosaminoglycan metachromasia from the cartilage matrix. These results are discussed in relation to current knowledge of metalloproteinase involvement in the chronic rheumatoid synovial pannus erosion of cartilage in humans. The data suggest that there are considerable differences between rheumatoid arthritis and these models, and their use must therefore be carefully defined.
- Published
- 1993
41. Changes with age in the non-collagenous proteins of human bone.
- Author
-
Dickson IR and Bagga MK
- Subjects
- Adolescent, Adult, Aged, Blood Proteins metabolism, Calcium metabolism, Child, Child, Preschool, Collagen metabolism, Humans, Hydroxyproline metabolism, Infant, Infant, Newborn, Middle Aged, Serum Albumin metabolism, Sialoglycoproteins metabolism, alpha-2-HS-Glycoprotein, Aging, Bone Matrix metabolism, Proteins metabolism
- Abstract
Little is known about the function of the non-collagenous proteins in the organic matrix of bone. Information about these proteins was obtained from studies of human cortical bone. Analysis of fractions of bone differing in density showed that 96% of the alpha 2HS-glycoprotein and 58% of the albumin was associated with the mineralized phase. A study of age-related changes in the composition of bone showed that the amounts present of alpha 2HS-glycoprotein, albumin, sialoprotein, soluble collagen and of EDTA-soluble protein were all higher in bone from children than in adults. In subjects aged over 60 y the content in bone of albumin, sialoprotein and of total protein was similar to that of children. These differences could not be correlated with age-related changes in the calcium/hydroxyproline ratio nor with previous reports of alterations in the crystal structure and the physical properties of human cortical bone. This suggests that these proteins are unlikely to be involved in stabilization of the mineralized matrix or in influencing the physical properties of bone; their function appears more likely to be related to bone formation and turnover.
- Published
- 1985
- Full Text
- View/download PDF
42. The degradation of collagen in pig synovium in vitro and the effect of colchicine.
- Author
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Fell HB, Lawrence CE, Bagga MR, Hembry RM, and Reynolds JJ
- Subjects
- Animals, Cells, Cultured, Hydrocortisone pharmacology, Indomethacin pharmacology, Swine, Synovial Membrane cytology, Synovial Membrane drug effects, Vinblastine pharmacology, Colchicine pharmacology, Collagen metabolism, Microbial Collagenase metabolism, Synovial Membrane metabolism
- Abstract
Colchicine induced a rapid destruction of the collagenous matrix of pig synovial explants in culture in the presence of serum. The most efficacious doses were 0.01-0.1 micrograms/ml (2.5 x 10(-8) M - 2.5 x 10(-7) M). The histological progression of the tissue breakdown induced by colchicine was very similar, although faster, to that described for other agents (Fell et al., 1986), with cells having basophilic nuclei accumulating in areas of fibril degradation. The loss of collagen correlated with an increase in collagenase production and at the peak of resorption (6 to 8 days) active collagenase was present in the culture media. Immunocytochemical methods demonstrated active collagenase bound to collagen fibrils after only 4 days in culture, before significant collagen degradation could be observed histologically. Collagen breakdown was completely inhibited by cortisol, and partially inhibited by indomethacin: only the inhibition by indomethacin could be reversed by exogenous prostaglandin E2. Vinblastine at a higher dose was as effective as colchicine but the lumicolchicines, which do not disrupt microtubules, were ineffective. Although the precise mechanism of action of colchicine is unknown, this culture system provides a useful in vitro model for increasing our understanding of the cellular mechanisms of tissue breakdown and for elucidating the roles of active collagenase and related metalloproteinases.
- Published
- 1989
- Full Text
- View/download PDF
43. The promotion and inhibition of collagen-breakdown in organ cultures of pig synovium: the requirement for serum components and the involvement of cyclic adenosine 3':5'-monophosphate (cAMP).
- Author
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Fell HB, Reynolds JJ, Lawrence CE, Bagga MR, and Glauert AM
- Subjects
- Animals, Culture Media, Hydroxyproline analysis, Microscopy, Electron, Organ Culture Techniques, Swine, Synovial Membrane cytology, Synovial Membrane ultrastructure, Collagen metabolism, Cyclic AMP metabolism, Microbial Collagenase metabolism, Synovial Membrane metabolism
- Abstract
In many pathological situations connective tissue cells acquire the ability to degrade the macromolecular components of their extracellular matrix. To study the destruction of collagen we used organ cultures of porcine synovial tissue. In the presence of 15% rabbit serum explants shrink considerably during 10-14 days, owing to early loss of interfibrillar material followed by retraction and local destruction of collagen fibres, partly by phagocytosis. These changes, and the release of latent collagenase into the medium, are largely inhibited by cortisol and partially by indomethacin. Collagen destruction can be greatly accelerated by the addition to the culture medium of one of the following: sodium fluoride, 3-isobutyl-1-methylxanthine, dibutyryl cyclic adenosine 3':5'-monophosphate or forskolin; these agents are known to affect cyclic adenosine monophosphate metabolism and our results suggest strongly that a change in the intracellular levels of cyclic adenosine monophosphate is a key-step in the process leading to the increased catabolism of collagen. With these compounds the destruction of collagen is largely extracellular; the histological changes and the increased levels of collagenase associated with the destruction can be prevented by cortisol and, except in the case of dibutyryl cyclic adenosine monophosphate, at least partially by indomethacin. Without serum only 3-isobutyl-1-methylxanthine sometimes causes drastic breakdown of collagen. This model system should be of great benefit in exploring the mechanisms involved in collagen destruction.
- Published
- 1986
- Full Text
- View/download PDF
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