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The effects of adding dried Lyophyllum decastes (DLR) or dried L. decastes by-products (DBY) on physicochemical properties and nutritional functions of extruded barley flour (BF) were analyzed. Results revealed a notable decrease in the degree of starch gelatinization in the DLR and DBY groups compared to the control group with nothing added. Gastrointestinal digestion simulation using the INFOGEST 2.0 method indicated that the antioxidant capacity of DBY was significantly higher than the BF group and DLR group (P 0.5, P < 0.05). In summary, the incorporation of L. decastes or its by-products effectively enhanced the quality and functional attributes of barley flour products. Notably, DBY exhibited superior antioxidant activity and modulation of the gut microbiota. These findings offer theoretical support for the development of extruded L. decastes-barley products.

3D printing, as an advanced and promising strategy for processing electrode for energy storage devices, such as supercapacitors and batteries, has garnered considerable interest in recent decades. The interest in 3D printed electrodes stems from its exceptional performance and manufacturing features, including customized sizes and shapes and the layer‐by‐layer processing principle, etc., especially integrating with MXene which allows the manufacturing of electrodes from different raw materials and possessing desired electrochemical properties. Herculean challenges, such as material compatibility of the printing inks, nondurable interfacial or bulk mechanical strength of the printed electrodes, and sometimes the low capacitance, lead to inferior electrochemical performance and hinder the practical applications of this promising technology. In this review, we firstly summarize the representative 3D printing methods, then, review the MXene‐based 3D printing electrodes made from different materials, and last, provide electrochemical performance of 3D printing MXene‐based electrodes for supercapacitors. Furthermore, based on a summary on the recent progress, an outlook on these promising electrodes for sustainable energy devices is provided. We anticipate that this review could provide some insights into overcoming the challenges and achieving more remarkable electrochemical performance of 3D printing supercapacitor electrodes and offer perspectives in the future for emerging energy devices. [ABSTRACT FROM AUTHOR]

Studying glacier changes is essential for understanding global glacier mass balance, patterns in climate change, and the potential consequences they may have. The glaciers of Geladandong Mountain serve as a crucial water source for the Yangtze River and its downstream regions. Any changes in the glacier at the river source will directly impact the ecological environment and water recycling process downstream. This study presents an approach for monitoring Geladandong Mountain glacier elevation changes using ICESat-2 data combined with a Binned Multilevel Regression Fitting Method (BMRFM). We analysed multi-year ICESat-2 data, considering various topographic features, to understand glaciers' annual and seasonal elevation changes. Our research reveals significant spatial heterogeneity in glacier elevation changes, influenced by altitude, slope, and aspect factors. It demonstrates the effectiveness of ICESat-2 in glacier monitoring, offering insights into the impacts of climate change on glacier dynamics. Moreover, the results indicate a predominant trend of ongoing melting at lower altitudes, with a small amount of accretion at higher elevations. Over the last two decades, the overall elevation change rate is −0.23 ± 0.12 metres per year. Seasonal analysis shows substantial glacier thickening from April to August, primarily due to increased precipitation offsetting elevated temperatures. This study offers a robust method for monitoring glaciers using satellite data, thereby advancing the precision and reliability of glacier research. [ABSTRACT FROM AUTHOR]

Lactic acid bacteria fermentation is a beneficial bioprocessing method that can improve the flavor, transform nutrients, and maintain the biological activity of foods. The aim of this study is to investigate the effects of Lactiplantibacillus plantarum dy-1 fermentation on the nutritional components, flavor and taste properties, and composition of saponin compounds and their hypolipidemic and antioxidant activities. The results suggested that the total polyphenol content increased, and the soluble polysaccharides and total saponin contents decreased in fermented bitter melon juice (FJ) compared with those in non-fermented bitter melon juice (NFJ). The determination of volatile flavor substances by GC-MS revealed that the response values of acetic acid, n-octanol, sedumol, etc., augmented significantly, and taste analysis with an electronic tongue demonstrated lower bitterness and higher acidity in FJ. Furthermore, UPLC-Q-TOF-MS/MS testing showed a significant decrease in bitter compounds, including momordicines I and II, and a significant increase in the active saponin momordicine U in the fermented bitter melon saponin group (FJBMS). The in vitro assays indicated that FJBMS exhibited similar antioxidant activities as the non-fermented bitter melon saponin group (NFBMS). The in vitro results show that both NFBMS and FJBMS, when used at 50 μg/mL, could significantly reduce fat accumulation and the malondialdehyde (MDA) content and increased the catalase (CAT) activity, while there was no significant difference in the bioactivities of NFBMS and FJBMS. In conclusion, Lactiplantibacillus plantarum dy-1 fermentation is an effective means to lower the bitterness value of bitter melon and preserve the well-known bioactivities of its raw materials, which can improve the edibility of bitter melon. [ABSTRACT FROM AUTHOR]

Non-POU domain-containing octamer-binding protein (NONO) is a multi-functional nuclear protein which belongs to the Drosophila behavior/human splicing (DBHS) protein family. NONO is known to regulate multiple important biological processes including host antiviral immune response. However, whether NONO can inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication is less well understood. In this study, we demonstrated that swine NONO (sNONO) inhibited PRRSV replication, via increasing expression of IFN-β, whereas NONO knockdown or knockout in PAM-KNU cells was more susceptible to PRRSV infection. As an IRF3 positive regulation factor, NONO promoted IFN-β expression by enhancing activation of IRF3. During PRRSV infection, NONO further up-regulated IRF3-mediated IFN-β expression by interacting with PRRSV N protein. Mechanistically, NONO functioned as a scaffold protein to detect PRRSV N protein and formed N-NONO-IRF3 complex in the nucleus. Interestingly, it was found that the NONO protein reversed the inhibitory effect of PRRSV N protein on type I IFN signaling pathway. Taken together, our study provides a novel mechanism for NONO to increase the IRF3-mediated IFN-β activation by interacting with the viral N protein to inhibit PRRSV infection. Author summary: Porcine reproductive and respiratory syndrome (PRRS), which is caused by porcine reproductive and respiratory syndrome virus (PRRSV), is a highly infectious disease and leads to huge economic losses to the global swine industry. Here, we reported that Non-POU domain-containing octamer-binding protein (NONO) significantly inhibited PRRSV replication and increased the expression of IFN-β. We found that NONO positively regulated IFN-β signaling pathway by targeting IRF3 and interacted with PRRSV N protein to promote activation of IFN-β, which reversed the inhibitory effect of PRRSV N protein on type I IFN signaling. We also found that NONO acted as a bridge to recruit PRRSV N and IRF3 to form N-NONO-IRF3 complex and enhanced activation of IFN-β signaling. Our study uncovers a novel mechanism for NONO to exert antiviral response through interacting with PRRSV N protein and IRF3 to inhibit PRRSV replication. [ABSTRACT FROM AUTHOR]

SUMMARY: Maintaining an optimal redox status is essential for plant growth and development, particularly when the plants are under stress. AT‐hook motif nuclear localized (AHL) proteins are evolutionarily conserved transcription factors in plants. Much of our understanding about this gene family has been derived from studies on clade A members. To elucidate the functions of clade B genes, we first analyzed their spatial expression patterns using transgenic plants expressing a nuclear localized GFP under the control of their promoter sequences. AHL1, 2, 6, 7, and 10 were further functionally characterized owing to their high expression in the root apical meristem. Through mutant analyses and transgenic studies, we showed that these genes have the ability to promote root growth. Using yeast one‐hybrid and dual luciferase assays, we demonstrated that AHL1, 2, 6, 7, and 10 are transcription regulators and this activity is required for their roles in root growth. Although mutants for these genes did not showed obvious defects in root growth, transgenic plants expressing their fusion proteins with the SRDX repressor motif exhibited a short‐root phenotype. Through transcriptome analysis, histochemical staining and molecular genetics experiments, we found that AHL10 maintains redox homeostasis via direct regulation of glutathione transferase (GST) genes. When the transcript level of GSTF2, a top‐ranked target of AHL10, was reduced by RNAi, the short‐root phenotype in the AHL10‐SRDX expressing plant was largely rescued. These results together suggest that AHL genes function redundantly in promoting root growth through direct regulation of redox homeostasis. [ABSTRACT FROM AUTHOR]