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162 results on '"Bai, Shiqie"'

13. Contribution of the Bacterial Community of Poorly Fermented-Oat Silage To Biogas Emission On the Qinghai Tibetan Plateau

Author

Liao, Chaosheng, primary, Tang, Xiaolong, additional, You, Minghong, additional, Zhang, Changbing, additional, Gou, Wenlong, additional, Zhao, Man, additional, Xie, Yixiao, additional, Chen, Shiyong, additional, Bai, Binqiang, additional, Hao, Lizhuang, additional, Chen, Chao, additional, Tondrob, Dorjeeh, additional, Qu, Guangpeng, additional, Yang, Shuqing, additional, Huang, Bo, additional, Li, Pi, additional, and Bai, Shiqie, additional

Published
2023
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17. Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers in Kengyilia melanthera

Author

Xiong, Yanli, Yang, Jian, Xiong, Yi, Zhao, Junming, Liu, Lin, Liu, Wei, Sha, Lina, Zhou, Jiqiong, You, Minghong, Li, Daxu, Lei, Xiong, Bai, Shiqie, and Ma, Xiao

Subjects
Plant Science
Abstract

As a typical psammophyte of the Triticeae, Kengyilia melanthera possesses high feeding potential and great utilization values in desertification control in the Qinghai-Tibet Plateau. However, few gene function and genetic studies have been performed in K. melanthera. In this study, single-molecule real-time sequencing technology was used to obtain the full-length transcriptome sequence of K. melanthera, following the functional annotation of transcripts and prediction of coding sequences (CDSs), transcription factors (TFs), and long noncoding RNA (lncRNA) sequences. Meanwhile, a total of 42,433 SSR loci were detected, with 5′-UTRs having the most SSR loci and trinucleotide being the most abundant type. In total, 108,399 SSR markers were designed, and 300 SSR markers were randomly selected for diversity verification of K. melanthera. A total of 49 polymorphic SSR markers were used to construct the genetic relationships of 56 K. melanthera accessions, among which 21 SSR markers showed good cross-species transferability among the related species. In conclusion, the full-length transcriptome sequence of the K. melanthera will assist gene prediction and promote molecular biology and genomics research, and the polymorphic SSR markers will promote molecular-assisted breeding and related research of K. melanthera and its relatives.

Published
2022

18. High-Altitude Genetic Selection and Genome-Wide Association Analysis of Yield-Related Traits in Elymus sibiricus L. Using SLAF Sequencing

Author

Zhang, Zongyu, Zheng, Yuying, Zhang, Junchao, Wang, Na, Wang, Yanrong, Liu, Wenhui, Bai, Shiqie, and Xie, Wengang

Subjects
Plant Science
Abstract

The genetic adaptations to harsh climatic conditions in high altitudes and genetic basis of important agronomic traits are poorly understood in Elymus sibiricus L. In this study, an association population of 210 genotypes was used for population structure, selective sweep analysis, and genome-wide association study (GWAS) based on 88,506 single nucleotide polymorphisms (SNPs). We found 965 alleles under the natural selection of high altitude, which included 7 hub genes involved in the response to UV, and flavonoid and anthocyanin biosynthetic process based on the protein–protein interaction (PPI) analysis. Using a mixed linear model (MLM), the GWAS test identified a total of 1,825 significant loci associated with 12 agronomic traits. Based on the gene expression data of two wheat cultivars and the PPI analysis, we finally identified 12 hub genes. Especially, in plant height traits, the top hub gene (TOPLESS protein) encoding auxins and jasmonic acid signaling pathway, shoot apical meristem specification, and xylem and phloem pattern formation was highly overexpressed. These genes might play essential roles in controlling the growth and development of E. sibiricus. Therefore, this study provides fundamental insights relevant to hub genes and will benefit molecular breeding and improvement in E. sibiricus and other Elymus species.

Published
2022

21. Phylogeographical Pattern and Population Evolution History of Indigenous Elymus sibiricus L. on Qinghai-Tibetan Plateau

Author

Han, Mengli, primary, Zhang, Jingxue, additional, Li, Daxu, additional, Sun, Shengnan, additional, Zhang, Changbing, additional, Zhang, Chuanjie, additional, Yan, Lijun, additional, Guan, Yongzhuo, additional, Chen, Lili, additional, Guo, Yuxia, additional, You, Minghong, additional, Gou, Wenlong, additional, Li, Xinrui, additional, Yan, Jiajun, additional, Bai, Shiqie, additional, and Yan, Xuebing, additional

Published
2022
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22. Selecting suitable shrub and herb species to revegetation from the perspective of root exudates: An implication for ecological restoration of desertification in an alpine meadow of the eastern Tibetan Plateau

Author

Huang, Qian, primary, Zeng, Kai, additional, Chen, Dongming, additional, Li, Qi, additional, Gu, Rui, additional, Bai, Yanfu, additional, Sun, Feida, additional, Zhou, Jiqiong, additional, Gao, Wuchao, additional, Ran, Zhuoya, additional, Peng, Yan, additional, Zhao, Junming, additional, Ma, Xiao, additional, Bai, Shiqie, additional, and Liu, Lin, additional

Published
2022
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27. Comparative Metabolomic Studies of Siberian Wildrye (Elymus sibiricus L.): A New Look at the Mechanism of Plant Drought Resistance.

Author

Yu, Qingqing, Xiong, Yi, Su, Xiaoli, Xiong, Yanli, Dong, Zhixiao, Zhao, Junming, Shu, Xin, Bai, Shiqie, Lei, Xiong, Yan, Lijun, and Ma, Xiao

Subjects
DROUGHT tolerance, DROUGHTS, CLIMATE change, DROUGHT management, RESTORATION ecology, GENE regulatory networks, COMPARATIVE studies
Abstract

Drought is one of the most important factors affecting plant growth and production due to ongoing global climate change. Elymus sibiricus has been widely applied for ecological restoration and reseeding of degraded grassland in the Qinghai–Tibetan Plateau (QTP) because of its strong adaptability to barren, salted, and drought soils. To explore the mechanism of drought resistance in E. sibiricus, drought-tolerant and drought-sensitive genotypes of E. sibiricus were used in metabolomic studies under simulated long-term and short-term drought stress. A total of 1091 metabolites were detected, among which, 27 DMs were considered to be the key metabolites for drought resistance of E. sibiricus in weighted gene co-expression network analysis (WGCNA). Ten metabolites, including 3-amino-2-methylpropanoic acid, coniferin, R-aminobutyrate, and so on, and 12 metabolites, including L-Proline, L-histidine, N-acetylglycine, and so on, showed differential accumulation patterns under short-term and long-term drought stress, respectively, and thus, could be used as biomarkers for drought-tolerant and drought-sensitive E. sibiricus. In addition, different metabolic accumulation patterns and different drought response mechanisms were also found in drought-tolerant and drought-sensitive genotypes of E. sibiricus. Finally, we constructed metabolic pathways and metabolic patterns for the two genotypes. This metabolomic study on the drought stress response of E. sibiricus can provide resources and a reference for the breeding of new drought-tolerant cultivars of E. sibiricus. [ABSTRACT FROM AUTHOR]

Published
2023
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28. Effect of Harvest Time on the Seed Yield and Quality of Kengyilia melanthera.

Author

Ling, Yao, Yuan, Shuai, Xiong, Yanli, Chen, Shuming, Feng, Junjie, Zhao, Junming, Zhang, Chenglin, Lei, Xiong, You, Minghong, Bai, Shiqie, and Ma, Xiao

Subjects
HARVESTING time, SEED yield, SEED harvesting, SEED quality, ACID phosphatase
Abstract

Kengyilia melanthera is one of the most important forages, and has received significant attention as a desirable ecological pioneer grass for conserving grasslands and mitigating degradation pressure in a region. It is widely distributed in the alpine sandy meadow zone of the Eastern Qinghai–Tibet Plateau (QTP). Therefore, determining the optimal harvest time of this species is critical. A two-year field experiment (2016–2017) was utilized in this study to evaluate the impact of eight harvest times on the seed yield and quality of K. melanthera 'Aba'. The results show that the fresh weight (FW), dry weight (DW), seed yield (SY), thousand-grain weight (TGW), accelerated aging germination percentage (AAGP), and dehydrogenase activity (DA) of seeds increased with the extension of harvest time in two trial years, while the water content (WC) of seeds decreased. In both trial years, the germination percentage (GEP), germination potential (GP), and acid phosphatase activity (APA) of seeds increased initially and then decreased with the extension of harvest time. The comprehensive evaluation of the membership function and hierarchical clustering revealed that the 30th day after peak anthesis was the optimal harvest time for both 2016 and 2017. During this time, FW, DW, SY, TGW, AAGP, DA, WC, GEP, GP, and APA reached their optimal value, with 0.815 g, 0.518 g, 1773.67 kg·hm−2, 143.81%, 66%, 16.23 µg·mL−1, 36.01%, 87%, 85%, and 2.50 nmol·min−1 in 2016 and 0.805 g, 0.515 g, 1847.42 kg·hm−2, 133.96%, 65%, 16.52 µg·mL−1, 36.04%, 86.5%, 83.5%, and 2.55 nmol·min−1 in 2017, respectively. This study uncovers several high-efficiency and long-term technological solutions for cultivating high yields and quality production of K. melanthera in East QTP. [ABSTRACT FROM AUTHOR]

Published
2023
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31. Physiological and Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.) Varieties to High Temperature Stress

Author

Li, Yingzhu, primary, Li, Xinrui, additional, Zhang, Jin, additional, Li, Daxu, additional, Yan, Lijun, additional, You, Minghong, additional, Zhang, Jianbo, additional, Lei, Xiong, additional, Chang, Dan, additional, Ji, Xiaofei, additional, An, Jinchan, additional, Li, Mingfeng, additional, Bai, Shiqie, additional, and Yan, Jiajun, additional

Published
2021
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33. Study on differentially expressed genes related to defoliation traits in two alfalfa varieties based on RNA-Seq

Author

Cheng Qiming, Jia Yushan, Chengdong Zhang, Bai Shiqie, Ping Li, Ge Gentu, and Liu Liying

Subjects
0301 basic medicine, Differentially expressed genes (DEGs), lcsh:QH426-470, lcsh:Biotechnology, Quantitative Trait Loci, RNA-Seq, Proteomics, Transcriptome, 03 medical and health sciences, Abscission, Gene Expression Regulation, Plant, lcsh:TP248.13-248.65, Genetics, Medicago sativa, Gene, Plant Proteins, biology, Alfalfa, fungi, High-Throughput Nucleotide Sequencing, food and beverages, biology.organism_classification, Plant Leaves, Plant Breeding, lcsh:Genetics, 030104 developmental biology, Defoliation, Plant hormone, DNA microarray, Biotechnology, Research Article
Abstract

Background Alfalfa (Medicago sativa) is a widely cultivated, essential commercial forage crop. The protein content in its leaves is the critical factor in determining the quality of alfalfa. Thus far, the understanding of the molecular mechanism of alfalfa defoliation traits remains unclear. The transcriptome database created by RNA-Seq is used to identify critical genes related to defoliation traits. Results In this study, we sequenced the transcriptomes of the Zhungeer variety (with easy leaf abscission) and WL319HQ variety (without easy leaf abscission). Among the identified 66,734 unigenes, 706 differentially expressed genes (DEGs) upregulated, and 392 unigenes downregulated in the Zhungeer vs WL319HQ leaf. KEGG pathway annotations showed that 8,414 unigenes were annotated to 87 pathways and contained 281 DEGs. Six DEGs belonging to the “Carotenoid biosynthesis”, “Plant hormone signal transduction” and “Circadian rhythm-plant” pathways involved in defoliation traits were identified and validated by RT-qPCR analyses. Conclusions This study used RNA-Seq to discover genes associated with defoliation traits between two alfalfa varieties. Our transcriptome data dramatically enriches alfalfa functional genomic studies. In addition, these data provide theoretical guidance for field production practice and genetic breeding, as well as references for future study of defoliation traits in alfalfa. Electronic supplementary material The online version of this article (10.1186/s12864-018-5180-1) contains supplementary material, which is available to authorized users.

Published
2018

34. Genomic survey sequencing, development and characterization of single- and multi-locus genomic SSR markers of Elymus sibiricus L

Author

Xiong Lei, Zhixiao Dong, Xiao Ma, Bai Shiqie, Wendan Wu, Jian Yang, Qingqing Yu, Wenhui Liu, Yi Xiong, and Yanli Xiong

Subjects
Genetic Markers, Elymus, DNA, Plant, Locus (genetics), Plant Science, Elymus sibiricus L, Genome, Loss of heterozygosity, Polymorphism (computer science), lcsh:Botany, Single-locus SSR, Genome size, Gene Library, Whole genome sequencing, Genetics, Expressed Sequence Tags, biology, food and beverages, Chromosome Mapping, Genetic Variation, High-Throughput Nucleotide Sequencing, Genomics, biology.organism_classification, lcsh:QK1-989, Genomic survey sequencing, Genetic diversity study, Genetic Loci, Marker development, GC-content, Genome, Plant, Microsatellite Repeats, Research Article
Abstract

Background Siberian wildrye (Elymus sibiricus L.) attracts considerable interest for grassland establishment and pasture recovery in the Qinghai-Tibet Plateau (QTP) due to its excellence in strong stress tolerance, high nutritional value and ease to cultivate. However, the lack of genomic information of E. sibiricus hampers its genetics study and breeding process. Results In this study, we performed a genome survey and developed a set of SSR markers for E. sibiricus based on Next-generation sequencing (NGS). We generated 469.17 Gb clean sequence which is 58.64× of the 6.86 Gb estimated genome size. We assembled a draft genome of 4.34 Gb which has 73.23% repetitive elements, a heterozygosity ratio of 0.01% and GC content of 45.68%. Based on the gnomic sequences we identified 67,833 SSR loci and from which four hundred were randomly selected to develop markers. Finally, 30 markers exhibited polymorphism between accessions and ten were identified as single-locus SSR. These newly developed markers along with previously reported 30 ones were applied to analyze genetic polymorphism among 27 wild E. sibiricus accessions. We found that single-locus SSRs are superior to multi-loci SSRs in effectiveness. Conclusions This study provided insights into further whole genome sequencing of E. sibiricus in strategy selection. The novel developed SSR markers will facilitate genetics study and breeding for Elymus species.

Published
2020

41. Genetic variability and structure of an important wild steppe grass Psathyrostachys juncea (Triticeae: Poaceae) germplasm collection from north and central Asia

Author

Xiong, Yi, primary, Xiong, Yanli, additional, Yu, Qingqing, additional, Zhao, Junming, additional, Lei, Xiong, additional, Dong, Zhixiao, additional, Yang, Jian, additional, Song, Sijia, additional, Peng, Yan, additional, Liu, Wenhui, additional, Bai, Shiqie, additional, and Ma, Xiao, additional

Published
2020
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46. Effects of LAB inoculant and cellulase on the fermentation quality and chemical composition of forage soybean silage prepared with corn stover.

Author

Cheng, Qiming, Li, Ping, Xiao, Bingxue, Yang, Fuyu, Li, Daxu, Ge, Gentu, Jia, Yushan, and Bai, Shiqie

Subjects
SILAGE, CORN stover, FORAGE plants, METABOLIZABLE energy values, FORAGE, CELLULASE, LACTIC acid bacteria, SOYBEAN
Abstract

The objective of the present study was to evaluate the silage quality of forage soybean‐corn stover silage in the south of China. Forage soybean were combined manually with corn stover by ratios of R1 (25:75), R2 (50:50) and R3 (75:25) in fresh weight (FM) and ensiled in bag silos for 60 days at room temperature (20–25°C). All silages were treated without (control) or with lactic acid bacteria (LAB) inoculant (G, Lactobacillus plantarum and Lactobacillus buchneri, 105 cfu/g, of FM), cellulase (C, 0.02% of FM) and C + G. R1 silage had appropriate V‐score value with high lactic acid content and low pH value and ammonia‐N (NH3‐N) content, undetectable levels of propionic acid (PA) and butyric acid (BA). C and C + G reduced undesirable microorganisms of aerobic bacteria, yeasts and molds, resulting in improved nutritive value of R2 silage, as indicated by lower neutral detergent fiber and acid detergent fiber contents, and higher crude protein content, in vitro ruminal dry matter digestibility, gross energy and metabolizable energy values as compared to the control. Additives did not improve the quality of R3 silage with high pH value and NH3‐N content, BA and PA contents and undesirable microorganism counts. These results confirmed that high‐quality silage produced from mixtures (25:75) of forage soybean and corn stover in the south of China; additives (especially for C + G) could enhance the silage quality of forage soybean‐corn stover (50:50); and high proportion (>75%) of forage soybean was difficult to ensile. [ABSTRACT FROM AUTHOR]

Published
2021
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