Search

Your search keyword '"Baigorri, R. [0000-0002-9197-5895]"' showing total 3 results
Start Over Author "Baigorri, R. [0000-0002-9197-5895]"
3 results on '"Baigorri, R. [0000-0002-9197-5895]"'

1. Alternative Polyadenylation and Salicylic Acid Modulate Root Responses to Low Nitrogen Availability

Author

Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Conesa, Carlos M. [0000-0002-3113-4576], Navarro Neila, Sara [0000-0002-9335-5665], de Lorenzo, L. [0000-0001-5422-3339], Hunt, Arthur G. [0000-0002-0008-4158], Baigorri, R. [0000-0002-9197-5895], Garcia-Mina, J. M. [0000-0001-6352-9612], Zamarreño, Angel M. [0000-0003-1966-0239], Sacristán, Soledad [0000-0002-5753-2644], del Pozo, J. C. [0000-0002-4113-457X], Conesa, Carlos M., Saez, Angela, Navarro Neila, Sara, de Lorenzo, L., Hunt, Arthur G., Sepúlveda, Edgar B., Baigorri, R., Garcia-Mina, J. M., Zamarreño, Angel M., Sacristán, Soledad, del Pozo, J. C., Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Conesa, Carlos M. [0000-0002-3113-4576], Navarro Neila, Sara [0000-0002-9335-5665], de Lorenzo, L. [0000-0001-5422-3339], Hunt, Arthur G. [0000-0002-0008-4158], Baigorri, R. [0000-0002-9197-5895], Garcia-Mina, J. M. [0000-0001-6352-9612], Zamarreño, Angel M. [0000-0003-1966-0239], Sacristán, Soledad [0000-0002-5753-2644], del Pozo, J. C. [0000-0002-4113-457X], Conesa, Carlos M., Saez, Angela, Navarro Neila, Sara, de Lorenzo, L., Hunt, Arthur G., Sepúlveda, Edgar B., Baigorri, R., Garcia-Mina, J. M., Zamarreño, Angel M., Sacristán, Soledad, and del Pozo, J. C.

Abstract

Nitrogen (N) is probably the most important macronutrient and its scarcity limits plant growth, development and fitness. N starvation response has been largely studied by transcriptomic analyses, but little is known about the role of alternative polyadenylation (APA) in such response. In this work, we show that N starvation modifies poly(A) usage in a large number of transcripts, some of them mediated by FIP1, a component of the polyadenylation machinery. Interestingly, the number of mRNAs isoforms with poly(A) tags located in protein-coding regions or 5'-UTRs significantly increases in response to N starvation. The set of genes affected by APA in response to N deficiency is enriched in N-metabolism, oxidation-reduction processes, response to stresses, and hormone responses, among others. A hormone profile analysis shows that the levels of salicylic acid (SA), a phytohormone that reduces nitrate accumulation and root growth, increase significantly upon N starvation. Meta-analyses of APA-affected and fip1-2-deregulated genes indicate a connection between the nitrogen starvation response and salicylic acid (SA) signaling. Genetic analyses show that SA may be important for preventing the overgrowth of the root system in low N environments. This work provides new insights on how plants interconnect different pathways, such as defense-related hormonal signaling and the regulation of genomic information by APA, to fine-tune the response to low N availability.

Published
2020

2. Role of cis-zeatin in root responses to phosphate starvation

Author

Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Comunidad de Madrid, Silva-Navas, J. [0000-0003-1567-1184], Conesa, Carlos M. [0000-0002-3113-4576], Saez, Angela [0000-0002-9189-4737], Navarro Neila, Sara [0000-0002-9335-5665], Garcia-Mina, J. M. [0000-0001-6352-9612], Zamarreño, Angel M. [0000-0003-1966-0239], Baigorri, R. [0000-0002-9197-5895], Swarup, Ranjan [0000-0002-6438-9188], del Pozo, J. C. [0000-0002-4113-457X], Silva-Navas, J., Conesa, Carlos M., Saez, Angela, Navarro Neila, Sara, Garcia-Mina, J. M., Zamarreño, Angel M., Baigorri, R., Swarup, Ranjan, del Pozo, J. C., Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Comunidad de Madrid, Silva-Navas, J. [0000-0003-1567-1184], Conesa, Carlos M. [0000-0002-3113-4576], Saez, Angela [0000-0002-9189-4737], Navarro Neila, Sara [0000-0002-9335-5665], Garcia-Mina, J. M. [0000-0001-6352-9612], Zamarreño, Angel M. [0000-0003-1966-0239], Baigorri, R. [0000-0002-9197-5895], Swarup, Ranjan [0000-0002-6438-9188], del Pozo, J. C. [0000-0002-4113-457X], Silva-Navas, J., Conesa, Carlos M., Saez, Angela, Navarro Neila, Sara, Garcia-Mina, J. M., Zamarreño, Angel M., Baigorri, R., Swarup, Ranjan, and del Pozo, J. C.

Abstract

Phosphate (Pi) is an essential nutrient for all organisms. Roots are underground organs, but the majority of the root biology studies have been done on root systems growing in the presence of light. Root illumination alters the Pi starvation response (PSR) at different intensities. Thus, we have analyzed morphological, transcriptional and physiological responses to Pi starvation in dark-grown roots. We have identified new genes and pathways regulated by Pi starvation that were not described previously. We also show that Pi-starved plants increase the cis-zeatin (cZ) : trans-zeatin (tZ) ratio. Transcriptomic analyses show that tZ preferentially represses cell cycle and PSR genes, whereas cZ induces genes involved in cell and root hair elongation and differentiation. In fact, cZ-treated seedlings show longer root system as well as longer root hairs compared with tZ-treated seedlings, increasing the total absorbing surface. Mutants with low cZ concentrations do not allocate free Pi in roots during Pi starvation. We propose that Pi-starved plants increase the cZ : tZ ratio to maintain basal cytokinin responses and allocate Pi in the root system to sustain its growth. Therefore, cZ acts as a PSR hormone that stimulates root and root hair elongation to enlarge the root absorbing surface and to increase Pi concentrations in roots.

Published
2019

3. The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Author

Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Téllez-Robledo, B. [0000-0002-0846-9992], Manzano, Concepción [0000-0003-1592-5068], Saez, Angela [0000-0002-9189-4737], Navarro Neila, Sara [0000-0002-9335-5665], Silva-Navas, J. [0000-0002-0938-910X], de Lorenzo, L. [0000-0001-5422-3339], González García, M. Paz [0000-0002-3739-3368], Toribio, René [0000-0002-4847-629X], Hunt, Arthur G. [0000-0002-0008-4158], Baigorri, R. [0000-0002-9197-5895], Casimiro, Ilda [0000-0002-5817-9176], Brady, Siobhan M. [0000-0001-9424-8055], Castellano Moreno, María Mar [0000-0003-0339-4247], del Pozo, J. C. [0000-0002-4113-457X], Téllez-Robledo, B., Manzano, Concepción, Saez, Angela, Navarro Neila, Sara, Silva-Navas, J., de Lorenzo, L., González García, M. Paz, Toribio, René, Hunt, Arthur G., Baigorri, R., Casimiro, Ilda, Brady, Siobhan M., Castellano Moreno, María Mar, del Pozo, J. C., Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Téllez-Robledo, B. [0000-0002-0846-9992], Manzano, Concepción [0000-0003-1592-5068], Saez, Angela [0000-0002-9189-4737], Navarro Neila, Sara [0000-0002-9335-5665], Silva-Navas, J. [0000-0002-0938-910X], de Lorenzo, L. [0000-0001-5422-3339], González García, M. Paz [0000-0002-3739-3368], Toribio, René [0000-0002-4847-629X], Hunt, Arthur G. [0000-0002-0008-4158], Baigorri, R. [0000-0002-9197-5895], Casimiro, Ilda [0000-0002-5817-9176], Brady, Siobhan M. [0000-0001-9424-8055], Castellano Moreno, María Mar [0000-0003-0339-4247], del Pozo, J. C. [0000-0002-4113-457X], Téllez-Robledo, B., Manzano, Concepción, Saez, Angela, Navarro Neila, Sara, Silva-Navas, J., de Lorenzo, L., González García, M. Paz, Toribio, René, Hunt, Arthur G., Baigorri, R., Casimiro, Ilda, Brady, Siobhan M., Castellano Moreno, María Mar, and del Pozo, J. C.

Abstract

Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5' untranslated regions (5'-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results