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Physical exercise is a tool to prevent and treat some of the chronic diseases affecting the world’s population. A mechanism through which exercise could exert beneficial effects in the body is by provoking alterations to the gut microbiota, an environmental factor that in recent years has been associated with numerous chronic diseases. Here we show that physical exercise performed by women to at least the degree recommended by the World Health Organization can modify the composition of gut microbiota. Using high-throughput sequencing of the 16s rRNA gene, eleven genera were found to be significantly different between active and sedentary women. Quantitative PCR analysis revealed higher abundance of health-promoting bacterial species in active women, including Faecalibacterium prausnitzii, Roseburia hominis and Akkermansia muciniphila. Moreover, body fat percentage, muscular mass and physical activity significantly correlated with several bacterial populations. In summary, we provide the first demonstration of interdependence between some bacterial genera and sedentary behavior parameters, and show that not only does the dose and type of exercise influence the composition of gut microbiota, but also the breaking of sedentary behavior. [ABSTRACT FROM AUTHOR]

Objective: Design of a questionnaire to determine the satisfaction and perceptions of patients with electronic prescription system. Methods: The questionnaire was developed in three phases. First, search and selection of questionnaire attributes. Second, analysis of the questionnaire by experts with a focus group methodology with pharmacists, doctors and nurses. Third, observational pilot study with 50 patients, users of electronic prescription system for at least one year. The internal consistency of the questionnaire, intraobserver reliability and validity were assessed. Results: The first and second phase resulted in a questionnaire that includes 5 attributes and 17 items. In the psychometric study, Croncach's alpha was 0.77 (CI95%: 0.66- 0.85), being the "satisfaction" attribute the one most valued. The intraobserver reliability had a correlation coefficient of 0.96 (CI95%: 0.91-0.98). The validity of criterion was confirmed through Spearman's correlation with a VAS scale. Convergent validity was statistically significant (p=0.04) for the level of studies and did not show a relation with the number of prescribed treatments. Conclusions: The designed questionnaire has adequate reliability and validity and can be a useful tool for work in community pharmacy and for use in other autonomous communities. [ABSTRACT FROM AUTHOR]