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To determine whether cycle training of sedentary subjects would increase the expression of the principle muscle glucose transporters, six volunteers completed 6 wk of progressively increasing intensity stationary cycle cycling.In vastus lateralis muscle biopsies, changes in expression of GLUT1, GLUT4, GLUT5, and GLUT12 were compared using quantitative immunoblots with specific protein standards. Regulatory pathway components were evaluated by immunoblots of muscle homogenates and immunohistochemistry of microscopic sections.GLUT1 was unchanged, GLUT4 increased 66%, GLUT12 increased 104%, and GLUT5 decreased 72%. A mitochondrial marker (cytochrome c) and regulators of mitochondrial biogenesis (peroxisome proliferator-activated receptor gamma coactivator 1 alpha and phospho-5'-adenosine monophosphate-activated protein kinase) were unchanged, but the muscle hypertrophy pathway component, phospho-mammalian target of rapamycin (mTOR), increased 83% after the exercise program. In baseline biopsies, GLUT4 by immunohistochemical techniques was 37% greater in Type I (slow twitch, red) muscle fibers, but the exercise training increased GLUT4 expression in Type II (fast twitch, white) fibers by 50%, achieving parity with the Type I fibers. Baseline phospho-mTOR expression was 50% higher in Type II fibers and increased more in Type II fibers (62%) with training but also increased in Type I fibers (34%).Progressive intensity stationary cycle training of previously sedentary subjects increased muscle insulin-responsive glucose transporters (GLUT4 and GLUT12) and decreased the fructose transporter (GLUT5). The increase in GLUT4 occurred primarily in Type II muscle fibers, and this coincided with activation of the mTOR muscle hypertrophy pathway. There was little impact on Type I fiber GLUT4 expression and no evidence of change in mitochondrial biogenesis.

This thesis presents a simple, meanline analysis of the impact of blade roughness on the mass flow, work coefficient, and efficiency of a three-stage axial compressor as a function of the location of fouling. First, an extensive review is presented on the state-of-the-art of measuring compressor degradation and on the impact of roughness on loss and deviation in a compressor cascade. The performance of a baseline, three-stage compressor, which has hydrodynamically smooth blades, is predicted. Using this baseline geometry, the influence of roughness in the front, middle and rear stages is calculated using empirical data for the enhanced losses and increased deviation, with a stage stacking technique. Influence coefficients that relate percentage changes in one parameter to percentage changes in other parameters are calculated. This analysis predicts that the most sensitive parameter for predicting fouling in the front stages is the percentage change in mass flow and the most sensitive parameter for predicting fouling in the rear stages is the efficiency. http://archive.org/details/analysisofsensit109452916 Lieutenant, United States Coast Guard Approved for public release; distribution is unlimited.