14 results on '"Balatonyi, B."'
Search Results
2. Preconditioning is a method that may reduce the negative side-effect of pneumoperitoneum
- Author
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Jávor, Szaniszló Z., primary, Shanava, K., additional, Hocsák, E., additional, Kürthy, M., additional, Lantos, J., additional, Borsiczky, B., additional, Takács, I., additional, Horváth, Sz., additional, Balatonyi, B., additional, Ferencz, S., additional, Ferencz, A., additional, Rőth, E., additional, and Wéber, Gy., additional
- Published
- 2010
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3. Transgastric small bowel resection by using hybrid technique — Experimental study
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Shanava, K., primary, Horváth, Sz., additional, Karl-Hermann, F., additional, Jávor, Sz., additional, Takács, I., additional, Balatonyi, B., additional, Ferencz, S., additional, Ferencz, A., additional, Rőth, E., additional, and Wéber, György, additional
- Published
- 2010
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4. Examination of Protective Effect of Ischemic Postconditioning After Small Bowel Autotransplantation
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Ferencz, A., primary, Takács, I., additional, Horváth, S., additional, Ferencz, S., additional, Jávor, S., additional, Fekecs, T., additional, Shanava, K., additional, Balatonyi, B., additional, and Wéber, G., additional
- Published
- 2010
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5. Ischaemic postconditioning reduces peroxide formation, cytokine expression and leukocyte activation in reperfusion injury after abdominal aortic surgery in rat model
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Sínay, L., primary, Kürthy, M., additional, Horváth, Sz., additional, Arató, E., additional, Shafiei, M., additional, Lantos, J., additional, Ferencz, S., additional, Bátor, A., additional, Balatonyi, B., additional, Verzár, Zs., additional, Sütő, B., additional, Kollár, L., additional, Wéber, Gy., additional, Rőth, E., additional, and Jancsó, G., additional
- Published
- 2008
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6. Image analysis of fibrosis in labial salivary glands of patients with systemic autoimmune diseases. Close correlation of lobular fibrosis to seropositive rheumatoid arthritis and increased anti-CCP and RF titres in the serum.
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Katona K, Farkas N, Kneif M, SütŐ G, Berki T, Balatonyi B, and Tornóczky T
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- Adult, Aged, Autoantibodies blood, Biomarkers blood, Female, Humans, Male, Middle Aged, Peptides, Cyclic immunology, Rheumatoid Factor immunology, Anti-Citrullinated Protein Antibodies blood, Arthritis, Rheumatoid immunology, Salivary Glands, Minor pathology, Sjogren's Syndrome blood, Sjogren's Syndrome immunology
- Abstract
Lobular fibrosis in labial salivary glands of patients with systemic autoimmune disease is a rarely examined and rather neglected histological change. Its significance and disease association is poorly understood. Our aim was to explore the clinical correlations of fibrosis in labial salivary gland samples using objective methods and laboratory parameters. Labial salivary gland samples from more than 300 patients over a 3-year period were selected from the archives of the pathology department, histologically examined, digitised, image analysed and statistically evaluated to identify the presence and intensity of lobular fibrosis, its relation to age, clinical diagnoses of systemic autoimmune disease and the presence of rheumatoid factor (RF), anti-cyclic citrullinated peptide (CCP), antinuclear antibodies (ANAs), and anti-dsDNA serum markers. Significant correlation was found between lobular fibrosis and the presence of autoimmune disease (p = 0.023), mainly seropositive rheumatoid arthritis (p < 0.001). Also significant association was found between the fibrosis and the presence of serum anti-CCP (p < 0.001) and IgA/IgG/IgM-RF (p < 0.001, p < 0.001 and p = 0.008, respectively). Significant association was explored between the anti-dsDNA positivity and the negative histology groups (p = 0.033) and between the ANA positivity and the inflammation only group (p = 0.021). The results suggest that lobular fibrosis tends to associate to certain systemic autoimmune diseases, mainly seropositive rheumatoid arthritis, and seems to be rare in labial salivary gland biopsies of autoimmune diseases characterised by presence of anti-dsDNA. The close correlation of ANA positivity and the inflammation only histology was not surprising, since the majority of patients (62%) have Sjögren's syndrome, known for its inflammatory infiltrate. These findings emphasise that evaluation of lobular fibrosis and inflammation in histological samples of labial salivary gland biopsies are equally important., (Copyright © 2018 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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7. Polymorphisms in glutathione S-transferase are risk factors for perioperative acute myocardial infarction after cardiac surgery: a preliminary study.
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Kovacs V, Gasz B, Balatonyi B, Jaromi L, Kisfali P, Borsiczky B, Jancso G, Marczin N, Szabados S, Melegh B, Nasri A, and Roth E
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- Alleles, Cardiac Surgical Procedures methods, Case-Control Studies, Female, Gene Frequency genetics, Genotype, Humans, Male, Middle Aged, Myocardial Infarction surgery, Risk Factors, Glutathione Transferase genetics, Myocardial Infarction genetics, Polymorphism, Genetic genetics
- Abstract
In the present study we explored glutathione S-transferase (GST) polymorphisms in selected patients who experienced accelerated myocardial injury following open heart surgery and compared these to a control group of patients without postoperative complications. 758 Patients were enrolled from which 132 patients were selected to genotype analysis according to exclusion criteria. Patients were divided into the following groups: Group I: control patients (n = 78) without and Group II.: study patients (n = 54) with evidence of perioperative myocardial infarction. Genotyping for GSTP1 A (Ile105Ile/Ala113Ala), B (Ile105Val/Ala113Ala) and C (Ile105Val/Ala113Val) alleles was performed by using real-time-PCR. The heterozygous AC allele was nearly three times elevated (18.5 vs. 7.7 %) in the patients who suffered postoperative myocardial infarction compared to controls. Contrary, we found allele frequency of 14.1 % for homozygous BB allele in the control group whereas no such allele combination was present in the study group. These preliminary results may suggest the protective role for the B and C alleles during myocardial oxidative stress whereas the A allele may represent predisposing risk for cellular injury in patients undergoing cardiac surgery.
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- 2014
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8. The role of the inhibition of glutathione-S-transferase in the protective mechanisms of ischemic postconditioning.
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Balatonyi B, Gasz B, Kovács V, Lantos J, Jancsó G, Marczin N, and Rőth E
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- Animals, Animals, Newborn, Apoptosis drug effects, Cell Survival drug effects, Cells, Cultured, Enzyme Inhibitors pharmacology, Ethacrynic Acid pharmacology, Flow Cytometry, Models, Biological, Myocardial Reperfusion Injury pathology, Myocytes, Cardiac drug effects, Myocytes, Cardiac pathology, Pilot Projects, Rats, Rats, Wistar, Glutathione Transferase antagonists & inhibitors, Glutathione Transferase physiology, Ischemic Postconditioning, Myocardial Reperfusion Injury enzymology, Myocardial Reperfusion Injury prevention & control, Myocytes, Cardiac enzymology
- Abstract
The antioxidant glutathione-S-transferase (GST) is a crucial determinant of the development of ischaemic-reperfusion (I/R) injury, and plays a pivotal role in the regulation of the mitogen activated protein kinase (MAPK) pathways involved in stress response and apoptosis. The aim of this study was to investigate whether inhibition of GST can abolish the benefit of ischaemic postconditioning (IPoC). A neonatal rat cardiomyocyte cell culture was prepared and divided into 6 groups: (I) control group without treatment; (II) cells exposed to simulated I/R; (III) simulated I/R (sI/R) with IPoC; (IV) ethacrynic acid (EA) alone; (V) sI/R with EA; and (VI) sI/R and IPoC together with EA. Viability of the cells was measured by MTT assay, the quantity of apoptotic cells was assessed by flow cytometry following annexin V-FITC - propidium-iodide double staining. The activation of JNK, p38, ERK/p42-p44 MAPKs, and GSK-3β protein kinase was determined by flow-cytometric assay. GST inhibition markedly increased the apoptosis and decreased the cell viability despite IPoC. The protective effect of IPoC was lost in GST-inhibited groups for all MAPKs and GSK-3β. GST activity is required for the survival of cultured cardiomyocytes under stress conditions. GST inhibition was associated with differential activation of MAP and the protein kinases regulating these pathways in the process of ischaemic postconditioning.
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- 2013
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9. Effect of a glutathione S-transferase inhibitor on oxidative stress and ischemia-reperfusion-induced apoptotic signalling of cultured cardiomyocytes.
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Röth E, Marczin N, Balatonyi B, Ghosh S, Kovács V, Alotti N, Borsiczky B, and Gasz B
- Abstract
Oxidative stress and ischemia-reperfusion (I/R) injury are crucial in the pathogenesis of cardiovascular diseases. The antioxidant glutathione S-transferase (GST) is responsible for the high-capacity metabolic inactivation of electrophilic compounds and toxic substrates. The main objective of the present study was to examine the effect of GST inhibition (with the administration of ethacrynic acid [EA]) on the viability and apoptosis of cardiomyocytes when these cells are exposed to various stress components of I/R and mitogen-activated protein kinase (c-Jun N-terminal kinase, p38 and extracellular signal-regulated kinase [ERK]) inhibitors. The primary culture of neonatal rat cardiomyocytes was divided into six experimental groups: control group of cells (group 1), cells exposed to H(2)O(2) (group 2), I/R (group 3), I/R and EA (group 4), H(2)O(2) coupled with EA (group 5), and EA alone (group 6). The viability of cardiomyocytes was determined using a colorimetric MTT assay. The apoptosis ratio was evaluated via fluorescein isothiocyanate-labelled annexin V and propidium iodide staining. c-Jun N-terminal kinase, p38, Akt/protein kinase B and ERK/p42-p44 transcription factors were monitored with flow cytometry. c-Jun N-terminal kinase activation increased due to GST inhibition during I/R. EA administration led to a significant increase in p38 activation following both H(2)O(2) treatment and I/R. ERK phosphorylation increased when GST was exposed to I/R. A pronounced decrease in Akt phosphorylation was observed when cells were cotreated with EA and H(2)O(2). GST plays an important role as a regulator of mitogen-activated protein kinase pathways in I/R injury.
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- 2011
10. Comparative immunohistochemical study of tissue integration of macroporous and laminar surgical meshes.
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Takács I, Horváth S, Molnár Á, Gáspár S, Hajós R, Meczker Á, Kóbor P, Lantos J, Jávor S, Balatonyi B, Szekeres G, Röth E, and Wéber G
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- Animals, Biocompatible Materials, Coated Materials, Biocompatible, Humans, Immunohistochemistry, Keratins metabolism, Ki-67 Antigen metabolism, Materials Testing, Microscopy, Electron, Scanning, Models, Animal, Polypropylenes, Rabbits, Silicones, Time Factors, Tissue Adhesions metabolism, Tissue Adhesions pathology, Tissue Adhesions prevention & control, Vascular Endothelial Growth Factor A metabolism, Wound Healing, Hernia, Ventral surgery, Surgical Mesh
- Abstract
Intraperitoneal surgical mesh implantation is required for laparoscopic ventral hernia repair. Composite meshes are well known in animal models and human practice. The aim of our study is to compare the biological behaviour of two different textured silicone-covered polypropylene meshes. Transmural abdominal wall defect was created in 40 rabbits and treated as follows: In 20 animals a polypropylene mesh with a laminar silicone covering (LSPP) and in the rest a macroporous textured mesh knitted of silicone-impregnated polypropylene filaments (MSPP) was applied. One and three weeks after implantation we evaluated the intraperitoneal adhesion formation of the mesh macroscopically, histologically and immunohistochemically to detect the reactive cells, especially inflammatory, endothelial and mesothelial cells, as well as their proliferative activity, and with Scanning Electron microscopy to visualize the surface of the meshes. The adhesion formation caused by the composites showed no statistical difference after one week although in the three weeks old samples the LSPP adhesion was significantly weaker than that of MSPP. As complications, serome formation in both groups, fistulas, abscesses, and sc. haematoma in the LSPP group were found. Only in MSPP containing tissues was the decrease of Ki-67 positive proliferating cells significant. A significant increase in VEGF expressing cells was observed only in MSPP containing three week old samples, suggesting better regulation of vascular growth in tissues surrounding the implants. In one week old specimens we observed an irregular proliferation of cytokeratin containing mesothelial cells in both group. The intraperitoneal surface of MSPP mesh was covered with neoperitoneum, while it was not regularly seen on LSPP mesh after three week.
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- 2011
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11. Protective effect of PACAP against doxorubicin-induced cell death in cardiomyocyte culture.
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Racz B, Reglodi D, Horvath G, Szigeti A, Balatonyi B, Roth E, Weber G, Alotti N, Toth G, and Gasz B
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- Animals, Cell Death physiology, Cells, Cultured, Humans, Myocytes, Cardiac cytology, Oxidative Stress drug effects, Pituitary Adenylate Cyclase-Activating Polypeptide chemistry, Protein Isoforms chemistry, Protein Isoforms pharmacology, Random Allocation, Rats, Rats, Wistar, Antibiotics, Antineoplastic pharmacology, Cell Death drug effects, Doxorubicin pharmacology, Myocytes, Cardiac drug effects, Pituitary Adenylate Cyclase-Activating Polypeptide pharmacology
- Abstract
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a widely distributed endogenous neuropeptide, also occurring in the cardiovascular system. Among others, PACAP has been suggested as a cardioprotective factor. It has been shown that PACAP inhibits cardiac fibrosis and protects cardiomyocytes against oxidative stress and in vitro ischemia/reperfusion. The aim of the present study was to investigate whether PACAP is protective in doxorubicin-induced cell death of cardiomyocytes. Cardiomyocytes were exposed to 1 µM doxorubicin for 24 h, which resulted in a marked reduction of cell viability and a parallel increase of apoptotic cells assessed by MTT test and annexin V/propidium iodide flow cytometry assay. Co-incubation with 20 nM PACAP increased cell viability and reduced the percentage of apoptotic cells. Furthermore, doxorubicin increased the activation of caspase-3 and decreased the phosphorylation of Bad, while simultaneous PACAP treatment reduced the caspase-3 activation and increased the level of phospho-Bad. In summary, our present results demonstrate that PACAP effectively protects cardiomyocytes against doxorubicin-induced apoptotic cell death.
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- 2010
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12. [Tissue integration of various silicone-coated polypropylene surgical mesh].
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Takács I, Horváth S, Balatonyi B, Jávor S, Molnár A, Gáspár S, Hajós R, Meczker A, Lantos J, Roth E, and Wéber G
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- Animals, Cell Adhesion, Foreign-Body Reaction physiopathology, Hernia, Abdominal surgery, Immunohistochemistry, Keratins analysis, Ki-67 Antigen analysis, Microscopy, Electron, Scanning, Models, Animal, Neovascularization, Physiologic, Rabbits, Vascular Endothelial Growth Factor A analysis, Abdominal Wall surgery, Biocompatible Materials, Cell Proliferation, Peritoneum physiology, Polypropylenes, Silicones, Surgical Mesh, Tissue Engineering methods
- Abstract
Introduction/aim: Laparoscopic ventral hernia repair requires a surgical mesh implanted in intraperitoneal position. The combined, double layer meshes are promising in animal models as well as in human practice. The aim of this study was to compare the biological behaviour of two different textured silicone covered polypropylene mesh., Materials and Methods: 3 × 4 cm big full thickness defect of the abdominal wall was created in New Zealand White rabbits. The defect was covered in 20 animals with a polypropylene mesh with laminar silicone layer on the visceral surface (LSPP), while the remaining 20 cases the defects were covered with a macroporous textured silicone impregnated polypropylene mesh (MSPP). Intraperitoneal adhesion formation and tissue ingrowth in the meshes were investigated. Immunohistochemistry was used to detect proliferation activity (Ki-67), neovascularization (VEGF), and to visualize mesothelial layer (CK) over the mesh. Scanning electron microscopy was used to investigate the visceral surface of the meshes., Results: While intraperitoneal adhesion formation showed no difference after 1 week, LSPP mesh induced significantly less adhesions after 21 days. The Ki-67 positivity was significantly lower and the number of the VEGF positive cells increased with time in the MSPP group, this was missing in the LSPP group. The thin neoperitoneum layer was detected over MSPP mesh only with CK antibody., Conclusion: The material and texture of the mesh are responsible for tissular incorporation which is in accordance with the generated foreign body reaction.
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- 2010
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13. [A new method for prosthetisation of vascular patients with lower limb amputation: initial experiences with osseointegration technique].
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Ferencz S, Mangold V, Dérczy K, Takács I, Balatonyi B, Horváth S, Jávor S, Brånemark R, Horváth OP, Roth E, and Wéber G
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- Bone Screws, Exercise Therapy methods, Female, Femur, Humans, Hungary, Male, Peripheral Vascular Diseases physiopathology, Reoperation, Titanium, Amputation, Surgical, Artificial Limbs, Osseointegration, Peripheral Vascular Diseases rehabilitation, Peripheral Vascular Diseases surgery, Thigh surgery, Weight-Bearing
- Abstract
Introduction/aims: Prostheses use for lower limb amputees is difficult, while the socket is hard, the prosthesis is heavy. Drawbacks of conventional prosthesis are mainly associated with the socket, therefore osseointegration technique is a promising solution, since it doesn't require a socket. Our aim was to introduce this technique in Hungary and extend indication for vascular patients., Methods: The method includes two operative and one rehabilitation phases: during first operation a titanium screw is fixed into the femoral bone marrow cavity, this connects to an abutment, which also penetrates the skin, making a direct connection between the femur and the prosthesis during the second intervention. During rehabilitation the patient makes loading exercises and learns to walk with new prosthesis., Results: This method was launched in Hungary in 2005. Two female amputees were operated on initially, their second surgery was performed in 2006 (when titanium screw was applied in the male patients, as well). Incorporation of titanium screw was exquisite, and rehabilitation was successful. One of our male patients died eight months after his first operation due to myocardial infarction., Conclusion: Based on our experiences, the osseointegration technique facilitates rehabilitation of vascular patients for prostheses use. Adequate follow-up and stable vascular diseases are not contraindications, although further clinical trials are needed to determine its indication.
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- 2009
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14. [Transvaginal cholecystectomy in animal model: first series in Hungary].
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Horváth S, Gál I, Rákóczi I, Jávor S, Balatonyi B, Takács I, Ferencz A, Ferencz S, and Wéber G
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- Animals, Cholecystectomy, Laparoscopic adverse effects, Endoscopy adverse effects, Female, Hungary, Models, Animal, Sus scrofa, Cholecystectomy, Laparoscopic methods, Endoscopy methods, Vagina
- Abstract
Introduction: The Natural Orifice Transluminal Endoscopic Surgery (NOTES) is the newest trend in minimally invasive surgery. Based on clinical experiences, transvaginal cholecystectomy causes less pain and operative stress, requires shorter hospitalization and allows patients to return quicker to normal activity., Materials and Methods: A transvaginal cholecystectomy was carried out using hybrid technique in animal model first time in Hungary. A 5 mm umbilical trocar was used for preparation of cystic artery and duct, clip application and gallbladder dissection. A transvaginally inserted 10 mm trocar was used for laparoscopic camera to follow the procedure. Gallbladder was fixed and secured with a special curved instrument inserted also transvaginally during the procedure. At the end of procedure the gallbladder was removed transvaginally., Results: Six transvaginal cholecystectomies was performed on pigs. The mean time of operations was 78 min (40-145 minutes). During the operations and the follow up period (3 months) no complications and mortality was detected., Conclusions: According to our experiences both procedures can be safely carried out on animal model, but further refinement of devices is necessary.
- Published
- 2009
- Full Text
- View/download PDF
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