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2. Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective

Author

European Commission, Centre National de la Recherche Scientifique (France), Commissariat à l’Energie Atomique et aux Energies Alternatives (France), Swiss National Science Foundation, Comunidad de Madrid, Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Junta de Comunidades de Castilla-La Mancha, Buerki-Thurnherr, Tina [0000-0003-3723-6562], Wick, Peter [0000-0002-0079-4344], Pelin, Marco [0000-0002-4306-7411], Tubaro, Aurelia [0000-0003-2773-2589], Candotto Carniel, F. [0000-0002-8277-4725], Tretiach, M. [0000-0002-0529-1359], Flahaut, E. [0000-0001-8344-6902], Iglesias, Daniel [0000-0002-1998-0518], Vázquez, Ester [0000-0003-3223-8024], Cellot, Giada [0000-0001-9198-8402], Ballerini, Laura [0000-0001-8420-0787], Castagnola, Valentina [0000-0002-8651-5958], Benfenati, Fabio [0000-0002-0653-8368], Armirotti, Andrea [0000-0002-3766-8755], Sallustrau, Antoine [0000-0001-7118-9224], Taran, Frédéric [0000-0001-5461-329X], Keck, Mathilde [0000-0003-4717-3446], Bussy, Cyrill [0000-0001-8870-443X], Vranic, Sandra [0000-0002-6653-7156], Kostarelos, Kostas [0000-0002-2224-6672], Navas Antón, José María [0000-0002-7644-8499], Mouchet, F. [0000-0003-1993-8448], Suarez-Merino, Blanca [0000-0002-0201-265X], Kanerva, Tomi [0000-0003-2245-1921], Prato, Maurizio [0000-0002-8869-8612], Fadeel, Bengt [0000-0001-5559-8482], Bianco, Alberto [0000-0002-1090-296X], Lin, Hazel, Buerki-Thurnherr, Tina, Kaur, Jasreen, Wick, Peter, Pelin, Marco, Tubaro, Aurelia, Candotto Carniel, F., Tretiach, M., Flahaut, E., Iglesias, Daniel, Vázquez, Ester, Cellot, Giada, Ballerini, Laura, Castagnola, Valentina, Benfenati, Fabio, Armirotti, Andrea, Sallustrau, Antoine, Taran, Frédéric, Keck, Mathilde, Bussy, Cyrill, Vranic, Sandra, Kostarelos, Kostas, Connolly, Mona, Navas Antón, José María, Mouchet, F., Gauthier, L., Baker, James, Suarez-Merino, Blanca, Kanerva, Tomi, Prato, Maurizio, Fadeel, Bengt, Bianco, Alberto, European Commission, Centre National de la Recherche Scientifique (France), Commissariat à l’Energie Atomique et aux Energies Alternatives (France), Swiss National Science Foundation, Comunidad de Madrid, Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Junta de Comunidades de Castilla-La Mancha, Buerki-Thurnherr, Tina [0000-0003-3723-6562], Wick, Peter [0000-0002-0079-4344], Pelin, Marco [0000-0002-4306-7411], Tubaro, Aurelia [0000-0003-2773-2589], Candotto Carniel, F. [0000-0002-8277-4725], Tretiach, M. [0000-0002-0529-1359], Flahaut, E. [0000-0001-8344-6902], Iglesias, Daniel [0000-0002-1998-0518], Vázquez, Ester [0000-0003-3223-8024], Cellot, Giada [0000-0001-9198-8402], Ballerini, Laura [0000-0001-8420-0787], Castagnola, Valentina [0000-0002-8651-5958], Benfenati, Fabio [0000-0002-0653-8368], Armirotti, Andrea [0000-0002-3766-8755], Sallustrau, Antoine [0000-0001-7118-9224], Taran, Frédéric [0000-0001-5461-329X], Keck, Mathilde [0000-0003-4717-3446], Bussy, Cyrill [0000-0001-8870-443X], Vranic, Sandra [0000-0002-6653-7156], Kostarelos, Kostas [0000-0002-2224-6672], Navas Antón, José María [0000-0002-7644-8499], Mouchet, F. [0000-0003-1993-8448], Suarez-Merino, Blanca [0000-0002-0201-265X], Kanerva, Tomi [0000-0003-2245-1921], Prato, Maurizio [0000-0002-8869-8612], Fadeel, Bengt [0000-0001-5559-8482], Bianco, Alberto [0000-0002-1090-296X], Lin, Hazel, Buerki-Thurnherr, Tina, Kaur, Jasreen, Wick, Peter, Pelin, Marco, Tubaro, Aurelia, Candotto Carniel, F., Tretiach, M., Flahaut, E., Iglesias, Daniel, Vázquez, Ester, Cellot, Giada, Ballerini, Laura, Castagnola, Valentina, Benfenati, Fabio, Armirotti, Andrea, Sallustrau, Antoine, Taran, Frédéric, Keck, Mathilde, Bussy, Cyrill, Vranic, Sandra, Kostarelos, Kostas, Connolly, Mona, Navas Antón, José María, Mouchet, F., Gauthier, L., Baker, James, Suarez-Merino, Blanca, Kanerva, Tomi, Prato, Maurizio, Fadeel, Bengt, and Bianco, Alberto

Abstract

Two-dimensional (2D) materials have attracted tremendous interest ever since the isolation of atomically thin sheets of graphene in 2004 due to the specific and versatile properties of these materials. However, the increasing production and use of 2D materials necessitate a thorough evaluation of the potential impact on human health and the environment. Furthermore, harmonized test protocols are needed with which to assess the safety of 2D materials. The Graphene Flagship project (2013-2023), funded by the European Commission, addressed the identification of the possible hazard of graphene-based materials as well as emerging 2D materials including transition metal dichalcogenides, hexagonal boron nitride, and others. Additionally, so-called green chemistry approaches were explored to achieve the goal of a safe and sustainable production and use of this fascinating family of nanomaterials. The present review provides a compact survey of the findings and the lessons learned in the Graphene Flagship.

Published
2024

9. Diverse Applications of Nanomedicine.

Author

Pelaz, Beatriz, Alexiou, Christoph, Alvarez-Puebla, Ramon A, Alves, Frauke, Andrews, Anne M, Ashraf, Sumaira, Balogh, Lajos P, Ballerini, Laura, Bestetti, Alessandra, Brendel, Cornelia, Bosi, Susanna, Carril, Monica, Chan, Warren CW, Chen, Chunying, Chen, Xiaodong, Chen, Xiaoyuan, Cheng, Zhen, Cui, Daxiang, Du, Jianzhong, Dullin, Christian, Escudero, Alberto, Feliu, Neus, Gao, Mingyuan, George, Michael, Gogotsi, Yury, Grünweller, Arnold, Gu, Zhongwei, Halas, Naomi J, Hampp, Norbert, Hartmann, Roland K, Hersam, Mark C, Hunziker, Patrick, Jian, Ji, Jiang, Xingyu, Jungebluth, Philipp, Kadhiresan, Pranav, Kataoka, Kazunori, Khademhosseini, Ali, Kopeček, Jindřich, Kotov, Nicholas A, Krug, Harald F, Lee, Dong Soo, Lehr, Claus-Michael, Leong, Kam W, Liang, Xing-Jie, Ling Lim, Mei, Liz-Marzán, Luis M, Ma, Xiaowei, Macchiarini, Paolo, Meng, Huan, Möhwald, Helmuth, Mulvaney, Paul, Nel, Andre E, Nie, Shuming, Nordlander, Peter, Okano, Teruo, Oliveira, Jose, Park, Tai Hyun, Penner, Reginald M, Prato, Maurizio, Puntes, Victor, Rotello, Vincent M, Samarakoon, Amila, Schaak, Raymond E, Shen, Youqing, Sjöqvist, Sebastian, Skirtach, Andre G, Soliman, Mahmoud G, Stevens, Molly M, Sung, Hsing-Wen, Tang, Ben Zhong, Tietze, Rainer, Udugama, Buddhisha N, VanEpps, J Scott, Weil, Tanja, Weiss, Paul S, Willner, Itamar, Wu, Yuzhou, Yang, Lily, Yue, Zhao, Zhang, Qian, Zhang, Qiang, Zhang, Xian-En, Zhao, Yuliang, Zhou, Xin, and Parak, Wolfgang J

Subjects
Animals, Humans, Neoplasms, Drug Carriers, Drug Delivery Systems, Particle Size, Nanotechnology, Nanomedicine, Nanoparticles, Bioengineering, Prevention, Biotechnology, 5.1 Pharmaceuticals, Generic Health Relevance, Nanoscience & Nanotechnology
Abstract

The design and use of materials in the nanoscale size range for addressing medical and health-related issues continues to receive increasing interest. Research in nanomedicine spans a multitude of areas, including drug delivery, vaccine development, antibacterial, diagnosis and imaging tools, wearable devices, implants, high-throughput screening platforms, etc. using biological, nonbiological, biomimetic, or hybrid materials. Many of these developments are starting to be translated into viable clinical products. Here, we provide an overview of recent developments in nanomedicine and highlight the current challenges and upcoming opportunities for the field and translation to the clinic.

Published
2017

10. Extracellular vesicles released by LPS-stimulated spinal organotypic slices spread neuroinflammation into naïve slices through connexin43 hemichannel opening and astrocyte aberrant calcium dynamics.

Author

Memo, Christian, Parisse, Pietro, Amoriello, Roberta, Pachetti, Maria, Palandri, Anabela, Casalis, Loredana, Ballerini, Clara, and Ballerini, Laura

Subjects
EXTRACELLULAR vesicles, NEUROINFLAMMATION, CONNEXIN 43, ATOMIC force microscopy, CALCIUM channels, CENTRAL nervous system
Abstract

Introduction: Neuroinflammation is a hallmark of multiple neurodegenerative diseases, shared by all pathological processes which primarily impact on neurons, including Central Nervous System (CNS) injuries. In reactive CNS, activated glia releases extracellular vesicles (EVs), nanosized membranous particles known to play a key role in intercellular communication. EVs mediate neuroinflammatory responses and might exacerbate tissue deterioration, ultimately influencing neurodegenerative disease progression. Methods: We treated spinal cord organotypic slices with LPS, a ligand extensively used to induce sEVs release, to mimic mild inflammatory conditions. We combine atomic force microscopy (AFM), nanoparticle tracking (NTA) and western blot (WB) analysis to validate the isolation and characterisation of sEVs. We further use immunofluorescence and confocal microscopy with live calcium imaging by GCaMP6f reporter to compare glial reactivity to treatments with sEVs when isolated from resting and LPS treated organ slices. Results: In our study, we focus on CNS released small EVs (sEVs) and their impact on the biology of inflammatory environment. We address sEVs local signalling within the CNS tissue, in particular their involvement in inflammation spreading mechanism(s). sEVs are harvested from mouse organotypic spinal cord cultures, an in vitro model which features 3D complexity and retains spinal cord resident cells. By confocal microscopy and live calcium imaging we monitor glial responses in naïve spinal slices when exposed to sEVs isolated from resting and LPS treated organ slices. Discussion: We show that sEVs, only when released during LPS neuroinflammation, recruit naïve astrocytes in the neuroinflammation cycle and we propose that such recruitment be mediated by EVs hemichannel (HC) permeability. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective

Author

Lin, Hazel, primary, Buerki-Thurnherr, Tina, additional, Kaur, Jasreen, additional, Wick, Peter, additional, Pelin, Marco, additional, Tubaro, Aurelia, additional, Carniel, Fabio Candotto, additional, Tretiach, Mauro, additional, Flahaut, Emmanuel, additional, Iglesias, Daniel, additional, Vázquez, Ester, additional, Cellot, Giada, additional, Ballerini, Laura, additional, Castagnola, Valentina, additional, Benfenati, Fabio, additional, Armirotti, Andrea, additional, Sallustrau, Antoine, additional, Taran, Frédéric, additional, Keck, Mathilde, additional, Bussy, Cyrill, additional, Vranic, Sandra, additional, Kostarelos, Kostas, additional, Connolly, Mona, additional, Navas, José Maria, additional, Mouchet, Florence, additional, Gauthier, Laury, additional, Baker, James, additional, Suarez-Merino, Blanca, additional, Kanerva, Tomi, additional, Prato, Maurizio, additional, Fadeel, Bengt, additional, and Bianco, Alberto, additional

Published
2024
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17. Graphene Oxide Nanosheets Reduce Astrocyte Reactivity to Inflammation and Ameliorate Experimental Autoimmune Encephalomyelitis [Dataset]

Author

Ballerini, Clara [clara.ballerini@unifi.it], Cellot, Giada [cellot@sissa.it], Ballerini, Laura [laura.ballerini@sissa.it], Di Mauro, Giuseppe, Amoriello, Roberta, Lozano, Neus, Carnasciali, Alberto, Guasti, Daniele, Becucci, Maurizio, Cellot, Giada, Kostarelos, Kostas, Ballerini, Clara, Ballerini, Laura, Ballerini, Clara [clara.ballerini@unifi.it], Cellot, Giada [cellot@sissa.it], Ballerini, Laura [laura.ballerini@sissa.it], Di Mauro, Giuseppe, Amoriello, Roberta, Lozano, Neus, Carnasciali, Alberto, Guasti, Daniele, Becucci, Maurizio, Cellot, Giada, Kostarelos, Kostas, Ballerini, Clara, and Ballerini, Laura

Abstract

In neuroinflammation, astrocytes play multifaceted roles that regulate the neuronal environment. Astrocytes sense and respond to pro-inflammatory cytokines (CKs) and, by a repertoire of intracellular Ca2+ signaling, contribute to disease progression. Therapeutic approaches wish to reduce the overactivation in Ca2+ signaling in inflammatory-reactive astrocytes to restore dysregulated cellular changes. Cell-targeting therapeutics might take advantage by the use of nanomaterial-multifunctional platforms such as graphene oxide (GO). GO biomedical applications in the nervous system involve therapeutic delivery and sensing, and GO flakes were shown to enable interfacing of neuronal and glial membrane dynamics. We exploit organotypic spinal cord cultures and optical imaging to explore Ca2+ changes in astrocytes, and we report, when spinal tissue is exposed to CKs, neuroinflammatory-associated modulation of resident glia. We show the efficacy of GO to revert these dynamic changes in astrocytic reactivity to CKs, and we translate this potential in an animal model of immune-mediated neuroinflammatory disease.

Published
2023

18. Graphene Oxide Nanosheets Reduce Astrocyte Reactivity to Inflammation and Ameliorate Experimental Autoimmune Encephalomyelitis

Author

European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Lozano, Neus [0000-0002-9026-1743], Cellot, Giada [0000-0001-9198-8402], Kostarelos, Kostas [0000-0002-2224-6672], Ballerini, Laura [0000-0001-8420-0787], Di Mauro, Giuseppe, Amoriello, Roberta, Lozano, Neus, Carnasciali, Alberto, Guasti, Daniele, Becucci, Maurizio, Cellot, Giada, Kostarelos, Kostas, Ballerini, Clara, Ballerini, Laura, European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Lozano, Neus [0000-0002-9026-1743], Cellot, Giada [0000-0001-9198-8402], Kostarelos, Kostas [0000-0002-2224-6672], Ballerini, Laura [0000-0001-8420-0787], Di Mauro, Giuseppe, Amoriello, Roberta, Lozano, Neus, Carnasciali, Alberto, Guasti, Daniele, Becucci, Maurizio, Cellot, Giada, Kostarelos, Kostas, Ballerini, Clara, and Ballerini, Laura

Abstract

In neuroinflammation, astrocytes play multifaceted roles that regulate the neuronal environment. Astrocytes sense and respond to pro-inflammatory cytokines (CKs) and, by a repertoire of intracellular Ca2+ signaling, contribute to disease progression. Therapeutic approaches wish to reduce the overactivation in Ca2+ signaling in inflammatory-reactive astrocytes to restore dysregulated cellular changes. Cell-targeting therapeutics might take advantage by the use of nanomaterial-multifunctional platforms such as graphene oxide (GO). GO biomedical applications in the nervous system involve therapeutic delivery and sensing, and GO flakes were shown to enable interfacing of neuronal and glial membrane dynamics. We exploit organotypic spinal cord cultures and optical imaging to explore Ca2+ changes in astrocytes, and we report, when spinal tissue is exposed to CKs, neuroinflammatory-associated modulation of resident glia. We show the efficacy of GO to revert these dynamic changes in astrocytic reactivity to CKs, and we translate this potential in an animal model of immune-mediated neuroinflammatory disease.

Published
2023

23. Adhesion to carbon nanotube conductive scaffolds forces action-potential appearance in immature rat spinal neurons.

Author

Fabbro, Alessandra, Sucapane, Antonietta, Toma, Francesca Maria, Calura, Enrica, Rizzetto, Lisa, Carrieri, Claudia, Roncaglia, Paola, Martinelli, Valentina, Scaini, Denis, Masten, Lara, Turco, Antonio, Gustincich, Stefano, Prato, Maurizio, and Ballerini, Laura

Subjects
Spinal Cord, Neurons, Animals, Rats, Nanotubes, Carbon, Cell Culture Techniques, Gene Expression Profiling, Cell Adhesion, Cell Differentiation, Gene Expression Regulation, Action Potentials, Tissue Scaffolds, Molecular Sequence Annotation, Nanotubes, Carbon, General Science & Technology
Abstract

In the last decade, carbon nanotube growth substrates have been used to investigate neurons and neuronal networks formation in vitro when guided by artificial nano-scaled cues. Besides, nanotube-based interfaces are being developed, such as prosthesis for monitoring brain activity. We recently described how carbon nanotube substrates alter the electrophysiological and synaptic responses of hippocampal neurons in culture. This observation highlighted the exceptional ability of this material in interfering with nerve tissue growth. Here we test the hypothesis that carbon nanotube scaffolds promote the development of immature neurons isolated from the neonatal rat spinal cord, and maintained in vitro. To address this issue we performed electrophysiological studies associated to gene expression analysis. Our results indicate that spinal neurons plated on electro-conductive carbon nanotubes show a facilitated development. Spinal neurons anticipate the expression of functional markers of maturation, such as the generation of voltage dependent currents or action potentials. These changes are accompanied by a selective modulation of gene expression, involving neuronal and non-neuronal components. Our microarray experiments suggest that carbon nanotube platforms trigger reparative activities involving microglia, in the absence of reactive gliosis. Hence, future tissue scaffolds blended with conductive nanotubes may be exploited to promote cell differentiation and reparative pathways in neural regeneration strategies.

Published
2013

32. Graphene oxide nanosheets hamper glutamate mediated excitotoxicity and protect neuronal survival in an in vitro stroke model

Author

European Commission, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), Tortella, Lorenza, Santini, Irene, Lozano, Neus, Kostarelos, Kostas, Cellot, Giada, Ballerini, Laura, European Commission, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Ciencia e Innovación (España), Tortella, Lorenza, Santini, Irene, Lozano, Neus, Kostarelos, Kostas, Cellot, Giada, and Ballerini, Laura

Abstract

Small graphene oxide (s-GO) nanosheets reversibly downregulate central nervous system (CNS) excitatory synapses, with potential developments as future therapeutic tools to treat neuro-disorders characterized by altered glutamatergic transmission. Excitotoxicity, namely cell death triggered by exceeding ambient glutamate fueling over-activation of excitatory synapses, is a pathogenic mechanism shared by several neural diseases, from ischemic stroke to neurodegenerative disorders. In this work, CNS cultures were exposed to oxygen-glucose deprivation (OGD) to mimic ischemic stroke in vitro, and it is show that the delivery of s-GO following OGD, during the endogenous build-up of secondary damage and excitotoxicity, improved neuronal survival. In a different paradigm, excitotoxicity cell damage was reproduced through exogenous glutamate application, and s-GO co-treatment protected neuronal integrity, potentially by directly downregulating the synaptic over-activation brought about by exogenous glutamate. This proof-of-concept study suggests that s-GO may find novel applications in therapeutic developments for treating excitotoxicity-driven neural cell death.

Published
2023

33. Delivery of graphene oxide nanosheets modulates glutamate release and normalizes amygdala synaptic plasticity to improve anxiety-related behavior

Author

European Commission, Generalitat de Catalunya, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Pati, Elisa, Biagioni, Audrey Franceschi, Casani, Raffaele, Lozano, Neus, Kostarelos, Kostas, Cellot, Giada, Ballerini, Laura, European Commission, Generalitat de Catalunya, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Pati, Elisa, Biagioni, Audrey Franceschi, Casani, Raffaele, Lozano, Neus, Kostarelos, Kostas, Cellot, Giada, and Ballerini, Laura

Abstract

Graphene oxide nanosheets (GO) were reported to alter neurobiological processes involving cell membrane dynamics. GO ability to reversibly downregulate specifically glutamatergic synapses underpins their potential in future neurotherapeutic developments. Aberrant glutamate plasticity contributes to stress-related psychopathology and drugs which target dysregulated glutamate represent promising treatments. We find that in a rat model of post-traumatic stress disorder (PTSD), a single injection of GO to the lateral amygdala following the stressful event induced PTSD-related behavior remission and reduced dendritic spine densities. We explored from a mechanistic perspective how GO could impair glutamate synaptic plasticity. By simultaneous patch clamp pair recordings of unitary synaptic currents, live-imaging of presynaptic vesicle release and confocal microscopy, we report that GO nanosheets altered the probability of release enhancing the extinction of synaptic plasticity in the amygdala. These findings show that the modulation of presynaptic glutamate release might represent an unexplored target for (nano)pharmacological interventions of stress-related disorders.

Published
2023

35. Carbon Nanotubes in Tissue Engineering

Author

Bosi, Susanna, Ballerini, Laura, Prato, Maurizio, Houk, Kendall N., Series editor, Hunter, Christopher A., Series editor, Ley, S.V., Series editor, Krische, Michael J, Series editor, Lehn, Jean-Marie, Series editor, Olivucci, Massimo, Series editor, Thiem, Joachim, Series editor, Venturi, Margherita, Series editor, Wong, Chi-Huey, Series editor, Wong, Henry N.C., Series editor, Marcaccio, Massimo, editor, and Paolucci, Francesco, editor

Published
2014
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36. MoS22D materials induce spinal cord neuroinflammation and neurotoxicity affecting locomotor performance in zebrafishElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4nh00041b

Author

Di Mauro, Giuseppe, González, Viviana Jehová, Bambini, Francesco, Camarda, Silvia, Prado, Eduardo, Holgado, Juan Pedro, Vázquez, Ester, Ballerini, Laura, and Cellot, Giada

Abstract

MoS2nanosheets belong to an emerging family of nanomaterials named bidimensional transition metal dichalcogenides (2D TMDCs). The use of such promising materials, featuring outstanding chemical and physical properties, is expected to increase in several fields of science and technology, with an enhanced risk of environmental dispersion and associated wildlife and human exposures. In this framework, the assessment of MoS2nanosheets toxicity is instrumental to safe industrial developments. Currently, the impact of the nanomaterial on the nervous tissue is unexplored. In this work, we use as in vivoexperimental model the early-stage zebrafish, to investigate whether mechano-chemically exfoliated MoS2nanosheets reach and affect, when added in the behavioral ambient, the nervous system. By high throughput screening of zebrafish larvae locomotor behavioral changes upon exposure to MoS2nanosheets and whole organism live imaging of spinal neuronal and glial cell calcium activity, we report that sub-acute and prolonged ambient exposures to MoS2nanosheets elicit locomotor abnormalities, dependent on dose and observation time. While 25 μg mL−1concentration treatments exerted transient effects, 50 μg mL−1ones induced long-lasting changes, correlated to neuroinflammation-driven alterations in the spinal cord, such as astrogliosis, glial intracellular calcium dysregulation, neuronal hyperactivity and motor axons retraction. By combining integrated technological approaches to zebrafish, we described that MoS22D nanomaterials can reach, upon water (i.e.ambient) exposure, the nervous system of larvae, resulting in a direct neurological damage.

Published
2024
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38. High-Performance Implantable Sensors based on Anisotropic Magnetoresistive La0.67Sr0.33MnO3 for Biomedical Applications

Author

Vera, Arturo, primary, Martínez, Isidoro, additional, Enger, Luiz Guilherme, additional, Guillet, Bruno, additional, Guerrero, Rubén, additional, Diez, José Manuel, additional, Rousseau, Olivier, additional, Lam Chok Sing, Marc, additional, Pierron, Victor, additional, Perna, Paolo, additional, Hernández, Jaime J., additional, Rodríguez, Isabel, additional, Calaresu, Ivo, additional, Meier, Anja, additional, Huck, Carmen, additional, Domínguez-Bajo, Ana, additional, González-Mayorga, Ankor, additional, López-Dolado, Elisa, additional, Serrano, María C., additional, Ballerini, Laura, additional, Pérez, Lucas, additional, Miranda, Rodolfo, additional, Flament, Stéphane, additional, González, María Teresa, additional, Méchin, Laurence, additional, and Camarero, Julio, additional

Published
2023
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43. Distributed interfacing by nanoscale photodiodes enables single-neuron light activation and sensory enhancement in 3D spinal explants

Author

Thalhammer, Agnes, Fontanini, Mario, Shi, Jiuyun, Scaini, Denis, Recupero, Luca, Evtushenko, Alexander, Fu, Ying, Pavagada, Suraj, Bistrovic-Popov, Andrea, Fruk, Ljiljana, Tian, Bozhi, Ballerini, Laura, Thalhammer, Agnes [0000-0002-4658-4174], Fontanini, Mario [0000-0001-7152-6243], Shi, Jiuyun [0000-0003-1949-0348], Scaini, Denis [0000-0001-8398-8074], Recupero, Luca [0000-0002-1442-4969], Fu, Ying [0000-0002-8196-6493], Pavagada, Suraj [0000-0001-9654-3867], Tian, Bozhi [0000-0003-0593-0023], Ballerini, Laura [0000-0001-8420-0787], and Apollo - University of Cambridge Repository

Subjects
FOS: Nanotechnology, mechanisms, Multidisciplinary, dorsal horn, interneurons, FOS: Clinical medicine, receptor, Neurosciences, 32 Biomedical and Clinical Sciences, Bioengineering, intrinsic silicon, cultures, Settore BIO/09 - Fisiologia, circuits, 3209 Neurosciences, Neurological, expression, Nanotechnology, QD, pain, patterns
Abstract

Among emerging technologies developed to interface neuronal signaling, engineering electrodes at the nanoscale would yield more precise biodevices opening to progress in neural circuit investigations and to new therapeutic potential. Despite remarkable progress in miniature electronics for less invasive neurostimulation, most nano-enabled, optically triggered interfaces are demonstrated in cultured cells, which precludes the studies of natural neural circuits. We exploit here free-standing silicon-based nanoscale photodiodes to optically modulate single, identified neurons in mammalian spinal cord explants. With near-infrared light stimulation, we show that activating single excitatory or inhibitory neurons differently affects sensory circuits processing in the dorsal horn. We successfully functionalize nano-photodiodes to target single molecules, such as glutamate AMPA receptor subunits, thus enabling light activation of specific synaptic pathways. We conclude that nano-enabled neural interfaces can modulate selected sensory networks with low invasiveness. The use of nanoscale photodiodes can thus provide original perspective in linking neural activity to specific behavioral outcome. We thank M. Gigante, G. Cellot, S. Mortal, and M. Lazzarino for help with setting up the laser stimulation setup; the group of P. Heppenstall for help with the AAV production; M. Giugliano for MATLAB code optimization; R. Owen for suggestions in antibody nPD characterization; and S. Usmani for help with initial experiments. This project was funded by HFSP program grant RGP0004/2019 (to L.B., B.T., and L.F.).

Published
2022

46. Distributed interfacing by nanoscale photodiodes enables single-neuron light activation and sensory enhancement in 3D spinal explants

Author

Farmacia y ciencias de los alimentos, Farmazia eta elikagaien zientziak, Thalhammer, Agnes, Fontanini, Mario, Shi, Jiuyun, Scaini, Denis, Recupero, Luca, Evtushenko, Alexander, Fu, Ying, Pavagada, Suraj, Bistrovic-Popov, Andrea, Fruk, Ljiljana, Tian, Bozhi, Ballerini, Laura, Farmacia y ciencias de los alimentos, Farmazia eta elikagaien zientziak, Thalhammer, Agnes, Fontanini, Mario, Shi, Jiuyun, Scaini, Denis, Recupero, Luca, Evtushenko, Alexander, Fu, Ying, Pavagada, Suraj, Bistrovic-Popov, Andrea, Fruk, Ljiljana, Tian, Bozhi, and Ballerini, Laura

Abstract

Among emerging technologies developed to interface neuronal signaling, engineering electrodes at the nanoscale would yield more precise biodevices opening to progress in neural circuit investigations and to new therapeutic potential. Despite remarkable progress in miniature electronics for less invasive neurostimulation, most nano-enabled, optically triggered interfaces are demonstrated in cultured cells, which precludes the studies of natural neural circuits. We exploit here free-standing silicon-based nanoscale photodiodes to optically modulate single, identified neurons in mammalian spinal cord explants. With near-infrared light stimulation, we show that activating single excitatory or inhibitory neurons differently affects sensory circuits processing in the dorsal horn. We successfully functionalize nano-photodiodes to target single molecules, such as glutamate AMPA receptor subunits, thus enabling light activation of specific synaptic pathways. We conclude that nano-enabled neural interfaces can modulate selected sensory networks with low invasiveness. The use of nanoscale photodiodes can thus provide original perspective in linking neural activity to specific behavioral outcome.

Published
2022

47. Nanotechnology-based neural interfaces for spinal cord reconnection

Author

Gonzalez, Maria Teresa, Rodilla, B.L., Calaresu, Ivo, Enger, Luiz Guilherme, Vera, Arturo, Dominguez-Bajo, A., Arché, A., Gonzalez-Mayorga, A., Hernandez, J., Meier, A., Ruiz-Gomez, S., Martinez, Isidoro, Guerrero, Ruben, Pierron, Victor, Lebargy, Sylvain, Guillet, Bruno, Lam Chok Sing, Marc, Rousseau, Olivier, Chaluvadi, Sandeep-Kumar, Huck, Carmen, Lecher, B., perez, lucas, Camarero, Julio, Flament, Stéphane, Scaini, Denis, Rodriguez, Isabella, Lopez-Dolado, Elisa, Serrano, Maria Conception, Méchin, Laurence, Ballerini, Laura, Miranda, Rodolfo, Instituto Imdea Nanociencia, Scuola Internazionale Superiore di Studi Avanzati / International School for Advanced Studies (SISSA / ISAS), Equipe Electronique - Laboratoire GREYC - UMR6072, Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU), SESCAM, MFD-Diagnostic, Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), This project has received funding from the European Union Horizon 2020 research and innovation program under grant agreement No 737116, and Méchin, Laurence

Subjects
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

48. Magnetic detection of neural activity in spinal cord slices

Author

Martinez, Isidoro, Vera, Arturo, Calaresu, Ivo, Guerrero, Ruben, Hernandez, J, Munoz, Vincente, Enger, Luiz Guilherme, Rodriguez, Isabella, Perez, Lucas, Camarero, Julio, Méchin, Laurence, Flament, Stéphane, Ballerini, Laura, Miranda, Rodolfo, Gonzalez, Maria Teresa, Instituto Imdea Nanociencia, Scuola Internazionale Superiore di Studi Avanzati / International School for Advanced Studies (SISSA / ISAS), Equipe Electronique - Laboratoire GREYC - UMR6072, Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU), and Méchin, Laurence

Subjects
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

49. High-Performance Implantable Sensors based on Anisotropic Magnetoresistive La0.67Sr0.33MnO3 for Biomedical Applications.

Author

Vera, Arturo, Martínez, Isidoro, Enger, Luiz Guilherme, Guillet, Bruno, Guerrero, Rubén, Diez, José Manuel, Rousseau, Olivier, Lam Chok Sing, Marc, Pierron, Victor, Perna, Paolo, Hernández, Jaime J., Rodríguez, Isabel, Calaresu, Ivo, Meier, Anja, Huck, Carmen, Domínguez-Bajo, Ana, González-Mayorga, Ankor, López-Dolado, Elisa, Serrano, María C., and Ballerini, Laura

Published
2023
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