1. Heterologous activation and metabolites identification of the pks7 gene cluster from Saccharopolyspora erythraea
- Author
-
Hao Tang, Xingchi Yang, Wenzong Wang, Xingjun Cui, Wenping Wei, Jing Wu, Peng Sun, and Bang-Ce Ye
- Subjects
Heterologous expression ,Saccharopolyspora erythraea ,6-Methylsalicyclic acid ,6-Ethylsalicyclic acid ,6-Ethylsalicyclic acid ester derivatives ,Flavonoid ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 - Abstract
The microbial genome remains a huge treasure trove for the discovery of diverse natural products. Saccharopolyspora erythraea NRRL23338, the industry producer of erythromycin, has a dozen of biosynthetic gene clusters whose encoding products are unidentified. Heterologous expression of one of the polyketide clusters pks7 in Streptomyces albus B4 chassis resulted in the characterization of its function responsible for synthesizing both 6-methylsalicyclic acid and 6-ethylsalicyclic acid. Meanwhile, two new 6-ethylsalicyclic acid ester derivatives were isolated as shunt metabolites. Their structures were identified by comprehensive analysis of MS and NMR experiments. Putative functions of genes within the pks7 BGC were also discussed.
- Published
- 2024
- Full Text
- View/download PDF