530 results on '"Baraban, Scott C."'
Search Results
2. Cell therapy for neurological disorders: Progress towards an embryonic medial ganglionic eminence progenitor-based treatment.
- Author
-
Righes Marafiga, Joseane and Baraban, Scott C
- Subjects
GABA ,epilepsy ,interneuron ,porcine ,progenitor cells ,Neurodegenerative ,Transplantation ,Epilepsy ,Regenerative Medicine ,Brain Disorders ,Stem Cell Research - Nonembryonic - Non-Human ,Neurosciences ,Stem Cell Research ,5.2 Cellular and gene therapies ,Development of treatments and therapeutic interventions ,Neurological ,Psychology ,Cognitive Sciences - Abstract
Impairment of development, migration, or function of inhibitory interneurons are key features of numerous circuit-based neurological disorders, such as epilepsy. From a therapeutic perspective, symptomatic treatment of these disorders often relies upon drugs or deep brain stimulation approaches to provide a general enhancement of GABA-mediated inhibition. A more effective strategy to target these pathological circuits and potentially provide true disease-modifying therapy, would be to selectively add new inhibitory interneurons into these circuits. One such strategy, using embryonic medial ganglionic (MGE) progenitor cells as a source of a unique sub-population of interneurons, has already proven effective as a cell transplantation therapy in a variety of preclinical models of neurological disorders, especially in mouse models of acquired epilepsy. Here we will discuss the evolution of this interneuron-based transplantation therapy in acquired epilepsy models, with an emphasis on the recent adaptation of MGE progenitor cells for xenotransplantation into larger mammals.
- Published
- 2023
3. Clemizole and trazodone are effective antiseizure treatments in a zebrafish model of STXBP1 disorder
- Author
-
Moog, Maia and Baraban, Scott C
- Subjects
Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Epilepsy ,Neurosciences ,Neurodegenerative ,Brain Disorders ,5.1 Pharmaceuticals ,Development of treatments and therapeutic interventions ,Animals ,Anticonvulsants ,Benzimidazoles ,Disease Models ,Animal ,Humans ,Larva ,Munc18 Proteins ,Random Allocation ,Seizures ,Trazodone ,Zebrafish ,antiepileptic ,electrophysiology ,seizure ,STXBP1 ,zebrafish ,Clinical sciences ,Biological psychology - Abstract
CRISPR-Cas9-generated zebrafish carrying a 12 base-pair deletion in stxbpb1b, a paralog sharing 79% amino acid sequence identity with human, exhibit spontaneous electrographic seizures during larval stages of development. Zebrafish stxbp1b mutants provide an efficient preclinical platform to test antiseizure therapeutics. The present study was designed to test antiseizure medications approved for clinical use and two recently identified repurposed drugs with antiseizure activity. Larval homozygous stxbp1b zebrafish (4 days postfertilization (dpf)) were agarose-embedded and monitored for electrographic seizure activity using a local field recording electrode placed in midbrain. Frequency of ictal-like events was evaluated at baseline and following 45 min of continuous drug exposure (1 mM, bath application). Analysis was performed on coded files by an experimenter blinded to drug treatment and genotype. Phenytoin (PHT), valproate (VPA), ethosuximide (ESX), levetiracetam (LEV), and diazepam (DZP) had no effect on the ictal-like event frequency in stxbp1b mutant zebrafish. Clemizole and trazodone decreased ictal-like event frequency in stxbp1b mutant zebrafish by 80% and 83%, respectively. These results suggest that repurposed drugs with serotonin receptor-binding affinities could be effective antiseizure treatments. Clemizole and trazodone were previously identified in a larval zebrafish model for Dravet syndrome. Based primarily on these preclinical zebrafish studies, compassionate-use and double-blind clinical trials with both drugs have progressed. The present study extends this approach to a preclinical zebrafish model representing STXBP1 (syntaxin-binding protein 1)-related disorders and suggests that future clinical studies may be warranted.
- Published
- 2022
4. Xenotransplantation of porcine progenitor cells in an epileptic California sea lion (Zalophus californianus): illustrative case.
- Author
-
Simeone, Claire A, Andrews, John P, Johnson, Shawn P, Casalia, Mariana, Kochanski, Ryan, Chang, Edward F, Cameron, Dianne, Dennison, Sophie, Inglis, Ben, Scott, Gregory, Kruse-Elliott, Kris, Okonski, F Fabian, Calvo, Eric, Goulet, Kelly, Robles, Dawn, Griffin-Stence, Ashley, Kuiper, Erin, Krasovec, Laura, Field, Cara L, Hoard, Vanessa F, and Baraban, Scott C
- Subjects
MGE progenitor cells ,domoic acid toxicosis ,epilepsy ,epilepsy xenograft ,interneuron progenitor cells ,sea lion ,Epilepsy ,Neurodegenerative ,Neurosciences ,Brain Disorders ,Neurological - Abstract
BackgroundDomoic acid (DA) is a naturally occurring neurotoxin harmful to marine animals and humans. California sea lions exposed to DA in prey during algal blooms along the Pacific coast exhibit significant neurological symptoms, including epilepsy with hippocampal atrophy.ObservationsHere the authors describe a xenotransplantation procedure to deliver interneuron progenitor cells into the damaged hippocampus of an epileptic sea lion with suspected DA toxicosis. The sea lion has had no evidence of seizures after the procedure, and clinical measures of well-being, including weight and feeding habits, have stabilized.LessonsThese preliminary results suggest xenotransplantation has improved the quality of life for this animal and holds tremendous therapeutic promise.
- Published
- 2022
5. Hippocampal gamma and sharp-wave ripple oscillations are altered in a Cntnap2 mouse model of autism spectrum disorder
- Author
-
Paterno, Rosalia, Marafiga, Joseane Righes, Ramsay, Harrison, Li, Tina, Salvati, Kathryn A, and Baraban, Scott C
- Subjects
Mental Health ,Brain Disorders ,Neurosciences ,Autism ,Intellectual and Developmental Disabilities (IDD) ,Basic Behavioral and Social Science ,Pediatric ,Prevention ,Behavioral and Social Science ,1.1 Normal biological development and functioning ,2.1 Biological and endogenous factors ,Aetiology ,Underpinning research ,Mental health ,Neurological ,Action Potentials ,Animals ,Autism Spectrum Disorder ,Disease Models ,Animal ,Gamma Rhythm ,Hippocampus ,Interneurons ,Male ,Membrane Proteins ,Mice ,Mice ,Inbred C57BL ,Mice ,Knockout ,Mice ,Transgenic ,Nerve Tissue Proteins ,Pyramidal Cells ,Spatial Behavior ,Synaptic Transmission ,EEG ,autism ,biomarker ,cntnap2 ,gamma ,hippocampus ,inhibition ,interneurons ,oscillations ,sharp wave ripples ,Biochemistry and Cell Biology ,Medical Physiology - Abstract
Impaired synaptic neurotransmission may underly circuit alterations contributing to behavioral autism spectrum disorder (ASD) phenotypes. A critical component of impairments reported in somatosensory and prefrontal cortex of ASD mouse models are parvalbumin (PV)-expressing fast-spiking interneurons. However, it remains unknown whether PV interneurons mediating hippocampal networks crucial to navigation and memory processing are similarly impaired. Using PV-labeled transgenic mice, a battery of behavioral assays, in vitro patch-clamp electrophysiology, and in vivo 32-channel silicon probe local field potential recordings, we address this question in a Cntnap2-null mutant mouse model representing a human ASD risk factor gene. Cntnap2-/- mice show a reduction in hippocampal PV interneuron density, reduced inhibitory input to CA1 pyramidal cells, deficits in spatial discrimination ability, and frequency-dependent circuit changes within the hippocampus, including alterations in gamma oscillations, sharp-wave ripples, and theta-gamma modulation. Our findings highlight hippocampal involvement in ASD and implicate interneurons as a potential therapeutical target.
- Published
- 2021
6. Maximally selective single-cell target for circuit control in epilepsy models
- Author
-
Hadjiabadi, Darian, Lovett-Barron, Matthew, Raikov, Ivan Georgiev, Sparks, Fraser T, Liao, Zhenrui, Baraban, Scott C, Leskovec, Jure, Losonczy, Attila, Deisseroth, Karl, and Soltesz, Ivan
- Subjects
Neurosciences ,Neurodegenerative ,Brain Disorders ,Epilepsy ,1.1 Normal biological development and functioning ,Underpinning research ,Aetiology ,2.1 Biological and endogenous factors ,Neurological ,Animals ,Cell Communication ,Dentate Gyrus ,Nerve Net ,Neurons ,Seizures ,Zebrafish ,adult-born granule cells ,calcium imaging ,effective connectivity modeling ,epilepsy ,higher-order organization ,hubs ,motifs ,network science ,seizure control ,single-cells ,Psychology ,Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Neurological and psychiatric disorders are associated with pathological neural dynamics. The fundamental connectivity patterns of cell-cell communication networks that enable pathological dynamics to emerge remain unknown. Here, we studied epileptic circuits using a newly developed computational pipeline that leveraged single-cell calcium imaging of larval zebrafish and chronically epileptic mice, biologically constrained effective connectivity modeling, and higher-order motif-focused network analysis. We uncovered a novel functional cell type that preferentially emerged in the preseizure state, the superhub, that was unusually richly connected to the rest of the network through feedforward motifs, critically enhancing downstream excitation. Perturbation simulations indicated that disconnecting superhubs was significantly more effective in stabilizing epileptic circuits than disconnecting hub cells that were defined traditionally by connection count. In the dentate gyrus of chronically epileptic mice, superhubs were predominately modeled adult-born granule cells. Collectively, these results predict a new maximally selective and minimally invasive cellular target for seizure control.
- Published
- 2021
7. Phenotypic analysis of catastrophic childhood epilepsy genes.
- Author
-
Griffin, Aliesha, Carpenter, Colleen, Liu, Jing, Paterno, Rosalia, Grone, Brian, Hamling, Kyla, Moog, Maia, Dinday, Matthew T, Figueroa, Francisco, Anvar, Mana, Ononuju, Chinwendu, Qu, Tony, and Baraban, Scott C
- Abstract
Genetic engineering techniques have contributed to the now widespread use of zebrafish to investigate gene function, but zebrafish-based human disease studies, and particularly for neurological disorders, are limited. Here we used CRISPR-Cas9 to generate 40 single-gene mutant zebrafish lines representing catastrophic childhood epilepsies. We evaluated larval phenotypes using electrophysiological, behavioral, neuro-anatomical, survival and pharmacological assays. Local field potential recordings (LFP) were used to screen ∼3300 larvae. Phenotypes with unprovoked electrographic seizure activity (i.e., epilepsy) were identified in zebrafish lines for 8 genes; ARX, EEF1A, GABRB3, GRIN1, PNPO, SCN1A, STRADA and STXBP1. We also created an open-source database containing sequencing information, survival curves, behavioral profiles and representative electrophysiology data. We offer all zebrafish lines as a resource to the neuroscience community and envision them as a starting point for further functional analysis and/or identification of new therapies.
- Published
- 2021
8. In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish
- Author
-
Liu, Jing, Salvati, Kathryn A, and Baraban, Scott C
- Subjects
Biomedical and Clinical Sciences ,Information and Computing Sciences ,Neurosciences ,Physical Sciences ,Machine Learning ,Intellectual and Developmental Disabilities (IDD) ,Neurodegenerative ,Epilepsy ,Brain Disorders ,Aetiology ,2.1 Biological and endogenous factors ,Underpinning research ,1.1 Normal biological development and functioning ,Neurological ,Cellular neuroscience ,Molecular neuroscience ,Optical imaging ,Criticality ,Network ,Calcium imaging ,Zebrafish - Abstract
STXBP1 mutations are associated with encephalopathy, developmental delay, intellectual disability, and epilepsy. While neural networks are known to operate at a critical state in the healthy brain, network behavior during pathological epileptic states remains unclear. Examining activity during periods between well-characterized ictal-like events (i.e., interictal period) could provide a valuable step toward understanding epileptic networks. To study these networks in the context of STXBP1 mutations, we combine a larval zebrafish model with in vivo fast confocal calcium imaging and extracellular local field potential recordings. Stxbp1b mutants display transient periods of elevated activity among local clusters of interacting neurons. These network "cascade" events were significantly larger in size and duration in mutants. At mesoscale resolution, cascades exhibit neurodevelopmental abnormalities. At single-cell scale, we describe spontaneous hyper-synchronized neuronal ensembles. That calcium imaging reveals uniquely disordered brain states during periods between pathological ictal-like seizure events is striking and represents a potential interictal biomarker.
- Published
- 2021
9. Interneuron Origins in the Embryonic Porcine Medial Ganglionic Eminence.
- Author
-
Casalia, Mariana L, Li, Tina, Ramsay, Harrison, Ross, Pablo J, Paredes, Mercedes F, and Baraban, Scott C
- Subjects
Median Eminence ,Ganglia ,Interneurons ,Animals ,Swine ,Rats ,Rats ,Sprague-Dawley ,Transplantation ,Heterologous ,Tissue Culture Techniques ,Female ,Male ,GABA ,development ,hippocampus ,interneuron ,porcine ,Stem Cell Research - Nonembryonic - Non-Human ,Neurosciences ,Stem Cell Research ,Mental Health ,Neurological ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Interneurons contribute to the complexity of neural circuits and maintenance of normal brain function. Rodent interneurons originate in embryonic ganglionic eminences, but developmental origins in other species are less understood. Here, we show that transcription factor expression patterns in porcine embryonic subpallium are similar to rodents, delineating a distinct medial ganglionic eminence (MGE) progenitor domain. On the basis of Nkx2.1, Lhx6, and Dlx2 expression, in vitro differentiation into neurons expressing GABA, and robust migratory capacity in explant assays, we propose that cortical and hippocampal interneurons originate from a porcine MGE region. Following xenotransplantation into adult male and female rat hippocampus, we further demonstrate that porcine MGE progenitors, like those from rodents, migrate and differentiate into morphologically distinct interneurons expressing GABA. Our findings reveal that basic rules for interneuron development are conserved across species, and that porcine embryonic MGE progenitors could serve as a valuable source for interneuron-based xenotransplantation therapies.SIGNIFICANCE STATEMENT Here we demonstrate that porcine medial ganglionic eminence, like rodents, exhibit a distinct transcriptional and interneuron-specific antibody profile, in vitro migratory capacity and are amenable to xenotransplantation. This is the first comprehensive examination of embryonic interneuron origins in the pig; and because a rich neurodevelopmental literature on embryonic mouse medial ganglionic eminence exists (with some additional characterizations in other species, e.g., monkey and human), our work allows direct neurodevelopmental comparisons with this literature.
- Published
- 2021
10. Enhancing glucose metabolism via gluconeogenesis is therapeutic in a zebrafish model of Dravet syndrome.
- Author
-
Banerji, Rajeswari, Huynh, Christopher, Figueroa, Francisco, Dinday, Matthew T, Baraban, Scott C, and Patel, Manisha
- Subjects
epilepsy ,gluconeogenesis ,metabolism ,mitochondria ,zebrafish - Abstract
Energy-producing pathways are novel therapeutic targets for the treatment of neurodevelopmental disorders. Here, we focussed on correcting metabolic defects in a catastrophic paediatric epilepsy, Dravet syndrome which is caused by mutations in sodium channel NaV1.1 gene, SCN1A. We utilized a translatable zebrafish model of Dravet syndrome (scn1lab) which exhibits key characteristics of patients with Dravet syndrome and shows metabolic deficits accompanied by down-regulation of gluconeogenesis genes, pck1 and pck2. Using a metabolism-based small library screen, we identified compounds that increased gluconeogenesis via up-regulation of pck1 gene expression in scn1lab larvae. Treatment with PK11195, a pck1 activator and a translocator protein ligand, normalized dys-regulated glucose levels, metabolic deficits, translocator protein expression and significantly decreased electrographic seizures in mutant larvae. Inhibition of pck1 in wild-type larvae mimicked metabolic and behaviour defects observed in scn1lab mutants. Together, this suggests that correcting dys-regulated metabolic pathways can be therapeutic in neurodevelopmental disorders such as Dravet syndrome arising from ion channel dysfunction.
- Published
- 2021
11. Phenotype-Based Screening of Synthetic Cannabinoids in a Dravet Syndrome Zebrafish Model
- Author
-
Griffin, Aliesha, Anvar, Mana, Hamling, Kyla, and Baraban, Scott C
- Subjects
Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Neurosciences ,Drug Abuse (NIDA only) ,Epilepsy ,Cannabinoid Research ,Brain Disorders ,Substance Misuse ,Neurodegenerative ,Aetiology ,2.1 Biological and endogenous factors ,Good Health and Well Being ,cannabinoid ,epilepsy ,locomotion ,screen ,seizure ,zebrafish ,Pharmacology and pharmaceutical sciences - Abstract
Dravet syndrome is a catastrophic epilepsy of childhood, characterized by cognitive impairment, severe seizures, and increased risk for sudden unexplained death in epilepsy (SUDEP). Although refractory to conventional antiepileptic drugs, emerging preclinical and clinical evidence suggests that modulation of the endocannabinoid system could be therapeutic in these patients. Preclinical research on this topic is limited as cannabis, delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD), are designated by United States Drug Enforcement Agency (DEA) as illegal substances. In this study, we used a validated zebrafish model of Dravet syndrome, scn1lab homozygous mutants, to screen for anti-seizure activity in a commercially available library containing 370 synthetic cannabinoid (SC) compounds. SCs are intended for experimental use and not restricted by DEA designations. Primary phenotype-based screening was performed using a locomotion-based assay in 96-well plates, and a secondary local field potential recording assay was then used to confirm suppression of electrographic epileptiform events. Identified SCs with anti-seizure activity, in both assays, included five SCs structurally classified as indole-based cannabinoids JWH 018 N-(5-chloropentyl) analog, JWH 018 N-(2-methylbutyl) isomer, 5-fluoro PB-22 5-hydroxyisoquinoline isomer, 5-fluoro ADBICA, and AB-FUBINACA 3-fluorobenzyl isomer. Our approach demonstrates that two-stage phenotype-based screening in a zebrafish model of Dravet syndrome successfully identifies SCs with anti-seizure activity.
- Published
- 2020
12. Network properties revealed during multi-scale calcium imaging of seizure activity in zebrafish
- Author
-
Liu, Jing and Baraban, Scott C
- Subjects
Information and Computing Sciences ,Biomedical and Clinical Sciences ,Neurosciences ,Machine Learning ,Epilepsy ,Neurodegenerative ,Brain Disorders ,1.1 Normal biological development and functioning ,Underpinning research ,Neurological ,Animals ,Animals ,Genetically Modified ,Brain ,Calcium ,Cortical Synchronization ,Microphthalmia-Associated Transcription Factor ,Neural Pathways ,Pentylenetetrazole ,Seizures ,Zebrafish ,Zebrafish Proteins ,epilepsy ,fast confocal ,neuronal networks ,synchronization ,whole-brain imaging ,zebrafish ,zebrafish - Abstract
Seizures are characterized by hypersynchronization of neuronal networks. Understanding these networks could provide a critical window for therapeutic control of recurrent seizure activity, i.e., epilepsy. However, imaging seizure networks has largely been limited to microcircuits in vitro or small "windows" in vivo. Here, we combine fast confocal imaging of genetically encoded calcium indicator (GCaMP)-expressing larval zebrafish with local field potential (LFP) recordings to study epileptiform events at whole-brain and single-neuron levels in vivo. Using an acute seizure model (pentylenetetrazole, PTZ), we reliably observed recurrent electrographic ictal-like events associated with generalized activation of all major brain regions and uncovered a well-preserved anterior-to-posterior seizure propagation pattern. We also examined brain-wide network synchronization and spatiotemporal patterns of neuronal activity in the optic tectum microcircuit. Brain-wide and single-neuronal level analysis of PTZ-exposed and 4-aminopyridine (4-AP)-exposed zebrafish revealed distinct network dynamics associated with seizure and non-seizure hyperexcitable states, respectively. Neuronal ensembles, comprised of coactive neurons, were also uncovered during interictal-like periods. Taken together, these results demonstrate that macro- and micro-network calcium motifs in zebrafish may provide a greater understanding of epilepsy.
- Published
- 2019
13. Zebrafish studies identify serotonin receptors mediating antiepileptic activity in Dravet syndrome.
- Author
-
Griffin, Aliesha L, Jaishankar, Priyadarshini, Grandjean, Jean-Marc, Olson, Steven H, Renslo, Adam R, and Baraban, Scott C
- Subjects
Chemical biology ,drug development ,electrophysiology ,epilepsy ,serotonin ,Neurodegenerative ,Neurosciences ,Epilepsy ,Brain Disorders ,2.1 Biological and endogenous factors ,5.1 Pharmaceuticals - Abstract
Dravet syndrome is a life-threatening early-onset epilepsy not well controlled by antiepileptic drugs. Drugs that modulate serotonin (5-HT) signalling, including clemizole, locaserin, trazodone and fenfluramine, have recently emerged as potential treatment options for Dravet syndrome. To investigate the serotonin receptors that could moderate this antiepileptic activity, we designed and synthesized 28 novel analogues of clemizole, obtained receptor binding affinity profiles, and performed in vivo screening in a scn1lab mutant zebrafish (Danio rerio) model which recapitulates critical clinical features of Dravet syndrome. We discovered three clemizole analogues with 5-HT receptor binding that exert powerful antiepileptic activity. Based on structure-activity relationships and medicinal chemistry-based analysis, we then screened an additional set of known 5-HT receptor specific drug candidates. Integrating our in vitro and in vivo data implicates 5-HT2B receptors as a critical mediator in the mechanism of seizure suppression observed in Dravet syndrome patients treated with 5-HT modulating drugs.
- Published
- 2019
14. Dlx1 and Dlx2 Promote Interneuron GABA Synthesis, Synaptogenesis, and Dendritogenesis
- Author
-
Pla, Ramon, Stanco, Amelia, Howard, MacKenzie A, Rubin, Anna N, Vogt, Daniel, Mortimer, Niall, Cobos, Inma, Potter, Gregory Brian, Lindtner, Susan, Price, James D, Nord, Alex S, Visel, Axel, Schreiner, Christoph E, Baraban, Scott C, Rowitch, David H, and Rubenstein, John LR
- Subjects
Biomedical and Clinical Sciences ,Neurosciences ,Genetics ,Pediatric ,Mental Health ,2.1 Biological and endogenous factors ,Animals ,Cerebral Cortex ,Female ,GABAergic Neurons ,Gene Expression Regulation ,Developmental ,Glutamate Decarboxylase ,Homeodomain Proteins ,Interneurons ,Male ,Mice ,Knockout ,Miniature Postsynaptic Potentials ,Synapses ,Transcription Factors ,Vesicular Inhibitory Amino Acid Transport Proteins ,gamma-Aminobutyric Acid ,cortex ,Dlx ,Gad ,interneuron ,synapse ,Psychology ,Cognitive Sciences ,Experimental Psychology ,Biological psychology ,Cognitive and computational psychology - Abstract
The postnatal functions of the Dlx1&2 transcription factors in cortical interneurons (CINs) are unknown. Here, using conditional Dlx1, Dlx2, and Dlx1&2 knockouts (CKOs), we defined their roles in specific CINs. The CKOs had dendritic, synaptic, and survival defects, affecting even PV+ CINs. We provide evidence that DLX2 directly drives Gad1, Gad2, and Vgat expression, and show that mutants had reduced mIPSC amplitude. In addition, the mutants formed fewer GABAergic synapses on excitatory neurons and had reduced mIPSC frequency. Furthermore, Dlx1/2 CKO had hypoplastic dendrites, fewer excitatory synapses, and reduced excitatory input. We provide evidence that some of these phenotypes were due to reduced expression of GRIN2B (a subunit of the NMDA receptor), a high confidence Autism gene. Thus, Dlx1&2 coordinate key components of CIN postnatal development by promoting their excitability, inhibitory output, and survival.
- Published
- 2018
15. Publisher Correction: PAFAH1B1 haploinsufficiency disrupts GABA neurons and synaptic E/I balance in the dentate gyrus.
- Author
-
Dinday, Matthew T, Girskis, Kelly M, Lee, Sunyoung, Baraban, Scott C, and Hunt, Robert F
- Subjects
Biochemistry and Cell Biology ,Other Physical Sciences - Abstract
A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper.
- Published
- 2018
16. Preclinical Animal Models for Dravet Syndrome: Seizure Phenotypes, Comorbidities and Drug Screening
- Author
-
Griffin, Aliesha, Hamling, Kyla R, Hong, SoonGweon, Anvar, Mana, Lee, Luke P, and Baraban, Scott C
- Subjects
Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Epilepsy ,Neurodegenerative ,Neurosciences ,Pediatric ,Genetics ,Brain Disorders ,Development of treatments and therapeutic interventions ,5.1 Pharmaceuticals ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,epilepsy ,dravet syndrome ,drug discovery ,in vivo ,precision medicine ,antiepileptic drugs ,animal models ,Pharmacology and pharmaceutical sciences - Abstract
Epilepsy is a common chronic neurological disease affecting almost 3 million people in the United States and 50 million people worldwide. Despite availability of more than two dozen FDA-approved anti-epileptic drugs (AEDs), one-third of patients fail to receive adequate seizure control. Specifically, pediatric genetic epilepsies are often the most severe, debilitating and pharmaco-resistant forms of epilepsy. Epileptic syndromes share a common symptom of unprovoked seizures. While some epilepsies/forms of epilepsy are the result of acquired insults such as head trauma, febrile seizure, or viral infection, others have a genetic basis. The discovery of epilepsy associated genes suggests varied underlying pathologies and opens the door for development of new "personalized" treatment options for each genetic epilepsy. Among these, Dravet syndrome (DS) has received substantial attention for both the pre-clinical and early clinical development of novel therapeutics. Despite these advances, there is no FDA-approved treatment for DS. Over 80% of patients diagnosed with DS carry a de novo mutation within the voltage-gated sodium channel gene SCN1A and these patients suffer with drug resistant and life-threatening seizures. Here we will review the preclinical animal models for DS featuring inactivation of SCN1A (including zebrafish and mice) with an emphasis on seizure phenotypes and behavioral comorbidities. Because many drugs fail somewhere between initial preclinical discovery and clinical trials, it is equally important that we understand how these models respond to known AEDs. As such, we will also review the available literature and recent drug screening efforts using these models with a focus on assay protocols and predictive pharmacological profiles. Validation of these preclinical models is a critical step in our efforts to efficiently discover new therapies for these patients. The behavioral and electrophysiological drug screening assays in zebrafish will be discussed in detail including specific examples from our laboratory using a zebrafish scn1 mutant and a summary of the nearly 3000 drugs screened to date. As the discovery and development phase rapidly moves from the lab-to-the-clinic for DS, it is hoped that this preclinical strategy offers a platform for how to approach any genetic epilepsy.
- Published
- 2018
17. Testing of putative antiseizure medications in a preclinical dravet syndrome zebrafish model
- Author
-
Whyte-Fagundes, Paige A, primary, Vance, Anjelica, additional, Carroll, Aloe, additional, Figueroa, Francisco, additional, Manukyan, Catherine, additional, and Baraban, Scott C, additional
- Published
- 2024
- Full Text
- View/download PDF
18. Persistent seizure control in epileptic mice transplanted with gamma‐aminobutyric acid progenitors
- Author
-
Casalia, Mariana L, Howard, MacKenzie A, and Baraban, Scott C
- Subjects
Biomedical and Clinical Sciences ,Neurosciences ,Stem Cell Research ,Epilepsy ,Transplantation ,Brain Disorders ,Stem Cell Research - Nonembryonic - Non-Human ,Neurodegenerative ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Animals ,Cell Differentiation ,Convulsants ,Disease Models ,Animal ,Embryo ,Mammalian ,Exploratory Behavior ,Inhibitory Postsynaptic Potentials ,Interneurons ,Male ,Median Eminence ,Mice ,Mice ,Transgenic ,Nuclear Proteins ,Pilocarpine ,Scopolamine ,Stem Cell Transplantation ,Stem Cells ,Thyroid Nuclear Factor 1 ,Transcription Factors ,gamma-Aminobutyric Acid ,Clinical Sciences ,Neurology & Neurosurgery ,Clinical sciences - Abstract
ObjectiveA significant proportion of the more than 50 million people worldwide currently suffering with epilepsy are resistant to antiepileptic drugs (AEDs). As an alternative to AEDs, novel therapies based on cell transplantation offer an opportunity for long-lasting modification of epileptic circuits. To develop such a treatment requires careful preclinical studies in a chronic epilepsy model featuring unprovoked seizures, hippocampal histopathology, and behavioral comorbidities.MethodsTransplantation of progenitor cells from embryonic medial or caudal ganglionic eminence (MGE, CGE) were made in a well-characterized mouse model of status epilepticus-induced epilepsy (systemic pilocarpine). Behavioral testing (handling and open field), continuous video-electroencephalographic (vEEG) monitoring, and slice electrophysiology outcomes were obtained up to 270 days after transplantation (DAT). Post-hoc immunohistochemistry was used to confirm cell identity.ResultsMGE progenitors transplanted into the hippocampus of epileptic mice rescued handling and open field deficits starting at 60 DAT. In these same mice, an 84% to 88% reduction in seizure activity was observed between 180 and 210 DAT. Inhibitory postsynaptic current frequency, measured on pyramidal neurons in acute hippocampal slices at 270 DAT, was reduced in epileptic mice but restored to naïve levels in epileptic mice receiving MGE transplants. No reduction in seizure activity was observed in epileptic mice receiving intrahippocampal CGE progenitors.InterpretationOur findings demonstrate that transplanted MGE progenitors enhance functional GABA-mediated inhibition, reduce spontaneous seizure frequency, and rescue behavioral deficits in a chronic epileptic animal model more than 6 months after treatment. Ann Neurol 2017;82:530-542.
- Published
- 2017
19. Mutations of conserved non-coding elements of PITX2 in patients with ocular dysgenesis and developmental glaucoma
- Author
-
Protas, Meredith E, Weh, Eric, Footz, Tim, Kasberger, Jay, Baraban, Scott C, Levin, Alex V, Katz, L Jay, Ritch, Robert, Walter, Michael A, Semina, Elena V, and Gould, Douglas B
- Subjects
Biological Sciences ,Bioinformatics and Computational Biology ,Genetics ,Congenital Structural Anomalies ,Pediatric ,Human Genome ,Eye Disease and Disorders of Vision ,Mental Health ,Neurodegenerative ,Aetiology ,2.1 Biological and endogenous factors ,Animals ,Anterior Eye Segment ,Conserved Sequence ,DNA Copy Number Variations ,Disease Models ,Animal ,Eye Abnormalities ,Gene Deletion ,Glaucoma ,Homeodomain Proteins ,Humans ,Introns ,Muscles ,Mutation ,Pedigree ,Sequence Deletion ,Transcription Factors ,Zebrafish ,Homeobox Protein PITX2 ,Medical and Health Sciences ,Genetics & Heredity - Abstract
Mutations in FOXC1 and PITX2 constitute the most common causes of ocular anterior segment dysgenesis (ASD), and confer a high risk for secondary glaucoma. The genetic causes underlying ASD in approximately half of patients remain unknown, despite many of them being screened by whole exome sequencing. Here, we performed whole genome sequencing on DNA from two affected individuals from a family with dominantly inherited ASD and glaucoma to identify a 748-kb deletion in a gene desert that contains conserved putative PITX2 regulatory elements. We used CRISPR/Cas9 to delete the orthologous region in zebrafish in order to test the pathogenicity of this structural variant. Deletion in zebrafish reduced pitx2 expression during development and resulted in shallow anterior chambers. We screened additional patients for copy number variation of the putative regulatory elements and found an overlapping deletion in a second family and in a potentially-ancestrally-related index patient with ASD and glaucoma. These data suggest that mutations affecting conserved non-coding elements of PITX2 may constitute an important class of mutations in patients with ASD for whom the molecular cause of their disease have not yet been identified. Improved functional annotation of the human genome and transition to sequencing of patient genomes instead of exomes will be required before the magnitude of this class of mutations is fully understood.
- Published
- 2017
20. PAFAH1B1 haploinsufficiency disrupts GABA neurons and synaptic E/I balance in the dentate gyrus.
- Author
-
Dinday, Matthew T, Girskis, Kelly M, Lee, Sunyoung, Baraban, Scott C, and Hunt, Robert F
- Subjects
Brain Disorders ,Neurodegenerative ,Epilepsy ,Intellectual and Developmental Disabilities ,Neurosciences ,Pediatric ,2.1 Biological and endogenous factors ,1.1 Normal biological development and functioning ,Neurological ,Biochemistry and Cell Biology ,Other Physical Sciences - Abstract
Hemizygous mutations in the human gene encoding platelet-activating factor acetylhydrolase IB subunit alpha (Pafah1b1), also called Lissencephaly-1, can cause classical lissencephaly, a severe malformation of cortical development. Children with this disorder suffer from deficits in neuronal migration, severe intellectual disability, intractable epilepsy and early death. While many of these features can be reproduced in Pafah1b1+/- mice, the impact of Pafah1b1+/- on the function of individual subpopulations of neurons and ultimately brain circuits is largely unknown. Here, we show tangential migration of young GABAergic interneurons into the developing hippocampus is slowed in Pafah1b1+/- mice. Mutant mice had a decreased density of parvalbumin- and somatostatin-positive interneurons in dentate gyrus, but no change in density of calretinin interneurons. Whole-cell patch-clamp recordings revealed increased excitatory and decreased inhibitory synaptic inputs onto granule cells of Pafah1b1+/- mice. Mutant animals developed spontaneous electrographic seizures, as well as long-term deficits in contextual memory. Our findings provide evidence of a dramatic shift in excitability in the dentate gyrus of Pafah1b1+/- mice that may contribute to epilepsy or cognitive impairments associated with lissencephaly.
- Published
- 2017
21. Behavioral Comorbidities and Drug Treatments in a Zebrafish scn1lab Model of Dravet Syndrome
- Author
-
Grone, Brian P, Qu, Tiange, and Baraban, Scott C
- Subjects
Biological Psychology ,Biological Sciences ,Biomedical and Clinical Sciences ,Psychology ,Brain Disorders ,Sleep Research ,Epilepsy ,Neurosciences ,Mental Health ,Basic Behavioral and Social Science ,Neurodegenerative ,Behavioral and Social Science ,Neurological ,Animals ,Animals ,Genetically Modified ,Anticonvulsants ,Cell Count ,Circadian Rhythm ,Cognitive Behavioral Therapy ,Disease Models ,Animal ,Epilepsies ,Myoclonic ,Exploratory Behavior ,Female ,Genotype ,Green Fluorescent Proteins ,Interneurons ,Larva ,Locomotion ,Male ,Mutation ,NAV1.1 Voltage-Gated Sodium Channel ,Sleep Wake Disorders ,Zebrafish ,Zebrafish Proteins ,anxiety ,Dravet syndrome ,epilepsy ,sleep ,sodium channels ,zebrafish - Abstract
Loss-of-function mutations in SCN1A cause Dravet syndrome (DS), a catastrophic childhood epilepsy in which patients experience comorbid behavioral conditions, including movement disorders, sleep abnormalities, anxiety, and intellectual disability. To study the functional consequences of voltage-gated sodium channel mutations, we use zebrafish with a loss-of-function mutation in scn1lab, a zebrafish homolog of human SCN1A. Homozygous scn1labs552/s552 mutants exhibit early-life seizures, metabolic deficits, and early death. Here, we developed in vivo assays using scn1labs552 mutants between 3 and 6 d postfertilization (dpf). To evaluate sleep disturbances, we monitored larvae for 24 h with locomotion tracking software. Locomotor activity during dark (night phase) was significantly higher in mutants than in controls. Among anticonvulsant drugs, clemizole and diazepam, but not trazodone or valproic acid, decreased distance moved at night for scn1labs552 mutant larvae. To monitor exploratory behavior in an open field, we tracked larvae in a novel arena. Mutant larvae exhibited impaired exploratory behavior, with increased time spent near the edge of the arena and decreased mobility, suggesting greater anxiety. Both clemizole and diazepam, but not trazodone or valproic acid, decreased distance moved and increased time spent in the center of the arena. Counting inhibitory neurons in vivo revealed no differences between scn1labs552 mutants and siblings. Taken together, our results demonstrate conserved features of sleep, anxiety, and movement disorders in scn1lab mutant zebrafish, and provide evidence that a zebrafish model allows effective tests of treatments for behavioral comorbidities associated with DS.
- Published
- 2017
22. Clemizole and modulators of serotonin signalling suppress seizures in Dravet syndrome.
- Author
-
Griffin, Aliesha, Hamling, Kyla R, Knupp, Kelly, Hong, SoonGweon, Lee, Luke P, and Baraban, Scott C
- Subjects
Epilepsy ,Neurosciences ,Brain Disorders ,Neurodegenerative ,Genetics ,Pediatric ,Development of treatments and therapeutic interventions ,5.1 Pharmaceuticals ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Adolescent ,Animals ,Animals ,Genetically Modified ,Anticonvulsants ,Benzazepines ,Benzimidazoles ,Child ,Disease Models ,Animal ,Epilepsies ,Myoclonic ,Female ,Gene Expression Regulation ,Developmental ,Humans ,Larva ,Male ,NAV1.1 Voltage-Gated Sodium Channel ,Protein Binding ,Receptors ,Serotonin ,Seizures ,Serotonin ,Signal Transduction ,Treatment Outcome ,Zebrafish ,epilepsy ,zebrafish ,drug-screening ,serotonin ,personalized medicine ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Dravet syndrome is a catastrophic childhood epilepsy with early-onset seizures, delayed language and motor development, sleep disturbances, anxiety-like behaviour, severe cognitive deficit and an increased risk of fatality. It is primarily caused by de novo mutations of the SCN1A gene encoding a neuronal voltage-activated sodium channel. Zebrafish with a mutation in the SCN1A homologue recapitulate spontaneous seizure activity and mimic the convulsive behavioural movements observed in Dravet syndrome. Here, we show that phenotypic screening of drug libraries in zebrafish scn1 mutants rapidly and successfully identifies new therapeutics. We demonstrate that clemizole binds to serotonin receptors and its antiepileptic activity can be mimicked by drugs acting on serotonin signalling pathways e.g. trazodone and lorcaserin. Coincident with these zebrafish findings, we treated five medically intractable Dravet syndrome patients with a clinically-approved serotonin receptor agonist (lorcaserin, Belviq®) and observed some promising results in terms of reductions in seizure frequency and/or severity. Our findings demonstrate a rapid path from preclinical discovery in zebrafish, through target identification, to potential clinical treatments for Dravet syndrome.
- Published
- 2017
23. Medial Ganglionic Eminence Progenitors Transplanted into Hippocampus Integrate in a Functional and Subtype-Appropriate Manner
- Author
-
Hsieh, Jui-Yi and Baraban, Scott C
- Subjects
Information and Computing Sciences ,Biomedical and Clinical Sciences ,Neurosciences ,Machine Learning ,Neurodegenerative ,Transplantation ,Brain Disorders ,Regenerative Medicine ,Mental Health ,Stem Cell Research - Nonembryonic - Non-Human ,Epilepsy ,Stem Cell Research ,Aetiology ,2.1 Biological and endogenous factors ,Neurological ,Animals ,Channelrhodopsins ,Embryo ,Mammalian ,Glutamate Decarboxylase ,Hippocampus ,In Vitro Techniques ,Luminescent Proteins ,Median Eminence ,Mice ,Mice ,Transgenic ,Neurons ,Optogenetics ,Parvalbumins ,Patch-Clamp Techniques ,Somatostatin ,Stem Cell Transplantation ,Stem Cells ,interneuron ,media ganglionic eminence ,optogenetics ,transplantation - Abstract
Medial ganglionic eminence (MGE) transplantation rescues disease phenotypes in various preclinical models with interneuron deficiency or dysfunction, including epilepsy. While underlying mechanism(s) remains unclear to date, a simple explanation is that appropriate synaptic integration of MGE-derived interneurons elevates GABA-mediated inhibition and modifies the firing activity of excitatory neurons in the host brain. However, given the complexity of interneurons and potential for transplant-derived interneurons to integrate or alter the host network in unexpected ways, it remains unexplored whether synaptic connections formed by transplant-derived interneurons safely mirror those associated with endogenous interneurons. Here, we combined optogenetics, interneuron-specific Cre driver mouse lines, and electrophysiology to study synaptic integration of MGE progenitors. We demonstrated that MGE-derived interneurons, when transplanted into the hippocampus of neonatal mice, migrate in the host brain, differentiate to mature inhibitory interneurons, and form appropriate synaptic connections with native pyramidal neurons. Endogenous and transplant-derived MGE progenitors preferentially formed inhibitory synaptic connections onto pyramidal neurons but not endogenous interneurons. These findings demonstrate that transplanted MGE progenitors functionally integrate into the postnatal hippocampal network.
- Published
- 2017
24. Interneuron deficits in neurodevelopmental disorders: Implications for disease pathology and interneuron-based therapies
- Author
-
Paterno, Rosalia, Casalia, Mariana, and Baraban, Scott C.
- Published
- 2020
- Full Text
- View/download PDF
25. Host brain environmental influences on transplanted medial ganglionic eminence progenitors
- Author
-
Paterno, Rosalia, primary, Vu, Thy, additional, Hsieh, Caroline, additional, and Baraban, Scott C., additional
- Published
- 2024
- Full Text
- View/download PDF
26. A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish.
- Author
-
Hong, SoonGweon, Lee, Philip, Baraban, Scott C, and Lee, Luke P
- Subjects
Animals ,Zebrafish ,Epilepsy ,Anticonvulsants ,Microfluidics ,Microelectrodes ,Electrophysiological Phenomena - Abstract
Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for electrophysiological studies. Using an electrode-integrated microfluidic system, here we demonstrate a novel multichannel electrophysiology unit to record multiple zebrafish. This platform allows spontaneous alignment of zebrafish and maintains, over days, close contact between head and multiple surface electrodes, enabling non-invasive long-term electroencephalographic recording. First, we demonstrate that electrographic seizure events, induced by pentylenetetrazole, can be reliably distinguished from eye or tail movement artifacts, and quantifiably identified with our unique algorithm. Second, we show long-term monitoring during epileptogenic progression in a scn1lab mutant recapitulating human Dravet syndrome. Third, we provide an example of cross-over pharmacology antiepileptic drug testing. Such promising features of this integrated microfluidic platform will greatly facilitate high-throughput drug screening and electrophysiological characterization of epileptic zebrafish.
- Published
- 2016
27. Altered Glycolysis and Mitochondrial Respiration in a Zebrafish Model of Dravet Syndrome.
- Author
-
Kumar, Maneesh G, Rowley, Shane, Fulton, Ruth, Dinday, Matthew T, Baraban, Scott C, and Patel, Manisha
- Subjects
Mitochondria ,Animals ,Animals ,Genetically Modified ,Zebrafish ,Epilepsies ,Myoclonic ,Disease Models ,Animal ,4-Aminopyridine ,Potassium Channel Blockers ,Histocompatibility Antigens ,Statistics ,Nonparametric ,Gene Expression Regulation ,Citric Acid Cycle ,Glycolysis ,Oxygen Consumption ,Larva ,Mutation ,NAV1.1 Voltage-Gated Sodium Channel ,Diet ,Ketogenic ,Dravet syndrome ,epilepsy ,glycolysis ,metabolism ,mitochondrial respiration ,zebrafish ,Genetically Modified ,Diet ,Ketogenic ,Disease Models ,Animal ,Epilepsies ,Myoclonic ,Statistics ,Nonparametric ,Neurosciences - Abstract
Altered metabolism is an important feature of many epileptic syndromes but has not been reported in Dravet syndrome (DS), a catastrophic childhood epilepsy associated with mutations in a voltage-activated sodium channel, Nav1.1 (SCN1A). To address this, we developed novel methodology to assess real-time changes in bioenergetics in zebrafish larvae between 4 and 6 d postfertilization (dpf). Baseline and 4-aminopyridine (4-AP) stimulated glycolytic flux and mitochondrial respiration were simultaneously assessed using a Seahorse Biosciences extracellular flux analyzer. Scn1Lab mutant zebrafish showed a decrease in baseline glycolytic rate and oxygen consumption rate (OCR) compared to controls. A ketogenic diet formulation rescued mutant zebrafish metabolism to control levels. Increasing neuronal excitability with 4-AP resulted in an immediate increase in glycolytic rates in wild-type zebrafish, whereas mitochondrial OCR increased slightly and quickly recovered to baseline values. In contrast, scn1Lab mutant zebrafish showed a significantly slower and exaggerated increase of both glycolytic rates and OCR after 4-AP. The underlying mechanism of decreased baseline OCR in scn1Lab mutants was not because of altered mitochondrial DNA content or dysfunction of enzymes in the electron transport chain or tricarboxylic acid cycle. Examination of glucose metabolism using a PCR array identified five glycolytic genes that were downregulated in scn1Lab mutant zebrafish. Our findings in scn1Lab mutant zebrafish suggest that glucose and mitochondrial hypometabolism contribute to the pathophysiology of DS.
- Published
- 2016
28. Epilepsy, Behavioral Abnormalities, and Physiological Comorbidities in Syntaxin-Binding Protein 1 (STXBP1) Mutant Zebrafish.
- Author
-
Grone, Brian P, Marchese, Maria, Hamling, Kyla R, Kumar, Maneesh G, Krasniak, Christopher S, Sicca, Federico, Santorelli, Filippo M, Patel, Manisha, and Baraban, Scott C
- Subjects
Animals ,Zebrafish ,Humans ,Epilepsy ,Disease Models ,Animal ,Zebrafish Proteins ,Behavior ,Animal ,Mutation ,Munc18 Proteins ,Behavior ,Animal ,Disease Models ,General Science & Technology - Abstract
Mutations in the synaptic machinery gene syntaxin-binding protein 1, STXBP1 (also known as MUNC18-1), are linked to childhood epilepsies and other neurodevelopmental disorders. Zebrafish STXBP1 homologs (stxbp1a and stxbp1b) have highly conserved sequence and are prominently expressed in the larval zebrafish brain. To understand the functions of stxbp1a and stxbp1b, we generated loss-of-function mutations using CRISPR/Cas9 gene editing and studied brain electrical activity, behavior, development, heart physiology, metabolism, and survival in larval zebrafish. Homozygous stxbp1a mutants exhibited a profound lack of movement, low electrical brain activity, low heart rate, decreased glucose and mitochondrial metabolism, and early fatality compared to controls. On the other hand, homozygous stxbp1b mutants had spontaneous electrographic seizures, and reduced locomotor activity response to a movement-inducing "dark-flash" visual stimulus, despite showing normal metabolism, heart rate, survival, and baseline locomotor activity. Our findings in these newly generated mutant lines of zebrafish suggest that zebrafish recapitulate clinical phenotypes associated with human syntaxin-binding protein 1 mutations.
- Published
- 2016
29. Testing of putative antiseizure drugs in a preclinical Dravet syndrome zebrafish model
- Author
-
Whyte-Fagundes, Paige, primary, Vance, Anjelica, additional, Carroll, Aloe, additional, Figueroa, Francisco, additional, Manukyan, Catherine, additional, and Baraban, Scott C, additional
- Published
- 2023
- Full Text
- View/download PDF
30. Interneuron Transplantation as a Treatment for Epilepsy.
- Author
-
Hunt, Robert F and Baraban, Scott C
- Subjects
Brain ,Interneurons ,Animals ,Humans ,Mice ,Disease Models ,Animal ,gamma-Aminobutyric Acid ,Stem Cell Transplantation ,Drug Resistant Epilepsy ,Neurosciences ,Epilepsy ,Transplantation ,Clinical Research ,Regenerative Medicine ,Stem Cell Research - Nonembryonic - Human ,Neurodegenerative ,Stem Cell Research ,Brain Disorders ,Stem Cell Research - Nonembryonic - Non-Human ,Aetiology ,2.1 Biological and endogenous factors ,Development of treatments and therapeutic interventions ,5.2 Cellular and gene therapies ,Neurological ,Medical Biochemistry and Metabolomics ,Medical Microbiology ,Medical Physiology - Abstract
Stem-cell therapy has extraordinary potential to address critical, unmet needs in the treatment of human disease. One particularly promising approach for the treatment of epilepsy is to increase inhibition in areas of the epileptic brain by grafting new inhibitory cortical interneurons. When grafted from embryos, young γ-aminobutyric acid (GABA)ergic precursors disperse, functionally mature into host brain circuits as local-circuit interneurons, and can stop seizures in both genetic and acquired forms of the disease. These features make interneuron cell transplantation an attractive new approach for the treatment of intractable epilepsies, as well as other brain disorders that involve increased risk for epilepsy as a comorbidity. Here, we review recent efforts to isolate and transplant cortical interneuron precursors derived from embryonic mouse and human cell sources. We also discuss some of the important challenges that must be addressed before stem-cell-based treatment for human epilepsy is realized.
- Published
- 2015
31. Large-Scale Phenotype-Based Antiepileptic Drug Screening in a Zebrafish Model of Dravet Syndrome
- Author
-
Dinday, Matthew T and Baraban, Scott C
- Subjects
antiepileptic ,drug discovery ,epilepsy ,high throughput ,pharmacology ,zebrafish ,Neurosciences - Abstract
Mutations in a voltage-gated sodium channel (SCN1A) result in Dravet Syndrome (DS), a catastrophic childhood epilepsy. Zebrafish with a mutation in scn1Lab recapitulate salient phenotypes associated with DS, including seizures, early fatality, and resistance to antiepileptic drugs. To discover new drug candidates for the treatment of DS, we screened a chemical library of ∼1000 compounds and identified 4 compounds that rescued the behavioral seizure component, including 1 compound (dimethadione) that suppressed associated electrographic seizure activity. Fenfluramine, but not huperzine A, also showed antiepileptic activity in our zebrafish assays. The effectiveness of compounds that block neuronal calcium current (dimethadione) or enhance serotonin signaling (fenfluramine) in our zebrafish model suggests that these may be important therapeutic targets in patients with DS. Over 150 compounds resulting in fatality were also identified. We conclude that the combination of behavioral and electrophysiological assays provide a convenient, sensitive, and rapid basis for phenotype-based drug screening in zebrafish mimicking a genetic form of epilepsy.
- Published
- 2015
32. NPAS1 represses the generation of specific subtypes of cortical interneurons.
- Author
-
Stanco, Amelia, Pla, Ramón, Vogt, Daniel, Chen, Yiran, Mandal, Shyamali, Walker, Jamie, Hunt, Robert F, Lindtner, Susan, Erdman, Carolyn A, Pieper, Andrew A, Hamilton, Steven P, Xu, Duan, Baraban, Scott C, and Rubenstein, John LR
- Subjects
Cerebral Cortex ,Interneurons ,Cells ,Cultured ,Animals ,Mice ,Inbred C57BL ,Animals ,Newborn ,Mice ,Transgenic ,Humans ,Mice ,Glutamate Decarboxylase ,Nerve Tissue Proteins ,Transcription Factors ,Autistic Disorder ,Age Factors ,Cell Proliferation ,MAP Kinase Signaling System ,Gene Expression Regulation ,Developmental ,Polymorphism ,Single Nucleotide ,Female ,Male ,Basic Helix-Loop-Helix Transcription Factors ,Embryo ,Mammalian ,LIM-Homeodomain Proteins ,Genetics ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research ,Underpinning research ,1.1 Normal biological development and functioning ,Neurosciences ,Psychology ,Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Little is known about genetic mechanisms that regulate the ratio of cortical excitatory and inhibitory neurons. We show that NPAS1 and NPAS3 transcription factors (TFs) are expressed in progenitor domains of the mouse basal ganglia (subpallium, MGE, and CGE). NPAS1(-/-) mutants had increased proliferation, ERK signaling, and expression of Arx in the MGE and CGE. NPAS1(-/-) mutants also had increased neocortical inhibition (sIPSC and mIPSC) and generated an excess of somatostatin(+) (SST) (MGE-derived) and vasoactive intestinal polypeptide(+) (VIP) (CGE-derived) neocortical interneurons, but had a normal density of parvalbumin(+) (PV) (MGE-derived) interneurons. In contrast, NPAS3(-/-) mutants showed decreased proliferation and ERK signaling in progenitors of the ganglionic eminences and had fewer SST(+) and VIP(+) interneurons. NPAS1 repressed activity of an Arx enhancer, and Arx overexpression resulted in increased proliferation of CGE progenitors. These results provide insights into genetic regulation of cortical interneuron numbers and cortical inhibitory tone.
- Published
- 2014
33. GABAB receptors in maintenance of neocortical circuit function
- Author
-
Sebe, Joy Y, Looke-Stewart, Elizabeth, and Baraban, Scott C
- Subjects
Biological Psychology ,Biomedical and Clinical Sciences ,Neurosciences ,Psychology ,Brain Disorders ,Mental Health ,Aetiology ,1.1 Normal biological development and functioning ,Underpinning research ,2.1 Biological and endogenous factors ,Neurological ,Animals ,Animals ,Newborn ,Calbindin 2 ,DNA-Binding Proteins ,Embryo ,Mammalian ,Green Fluorescent Proteins ,In Vitro Techniques ,Mice ,Mice ,Inbred BALB C ,Mice ,Transgenic ,Neocortex ,Nerve Net ,Neuropeptide Y ,Neurotransmitter Uptake Inhibitors ,Nipecotic Acids ,Oximes ,Piperidines ,Pyrimidines ,Receptors ,GABA-B ,Stem Cell Transplantation ,Synaptic Potentials ,Transcription Factors ,gamma-Aminobutyric Acid ,GABAB ,Tonic inhibition ,MGE ,Clinical Sciences ,Neurology & Neurosurgery ,Biological psychology - Abstract
Activation of metabotropic GABAB receptors (GABABRs) enhances tonic GABA current and substantially increases the frequency of spontaneous seizures. Despite the and pro-epileptic consequences of GABABR activation, mice lacking functional GABAB receptors (GABAB1R KO mice) exhibit clonic and rare absence seizures. To examine these mutant mice further, we recorded excitatory and inhibitory synaptic inputs and tonic mutant GABA currents from Layer 2 neocortical pyramidal neurons of GABAB1R WT and KO mice (P30-40). Tonic current was increased while the frequency of synaptic inputs was unchanged in KO mice relative to WT littermates. The neocortical laminar distribution of interneuron subtypes derived from the medial ganglionic eminence (MGE) was also not statistically different in KO mice relative to WT while the number of calretinin-positive, caudal GE-derived cells in Layer 1 was reduced. Transplantation of MGE progenitors obtained from KO mice lacking functional GABAB1R did not increase tonic inhibition in the host brain above that of media-injected controls. Taken together, these results suggest a complex role for GABAB receptors in mediating neocortical circuit function.
- Published
- 2014
34. 14-3-3ε and ζ Regulate Neurogenesis and Differentiation of Neuronal Progenitor Cells in the Developing Brain
- Author
-
Toyo-oka, Kazuhito, Wachi, Tomoka, Hunt, Robert F, Baraban, Scott C, Taya, Shinichiro, Ramshaw, Hayley, Kaibuchi, Kozo, Schwarz, Quenten P, Lopez, Angel F, and Wynshaw-Boris, Anthony
- Subjects
Brain Disorders ,Regenerative Medicine ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research ,Neurosciences ,1.1 Normal biological development and functioning ,Underpinning research ,Neurological ,14-3-3 Proteins ,Actins ,Animals ,Catenins ,Cell Movement ,Cell Proliferation ,Cerebral Cortex ,Mice ,Mice ,Inbred C57BL ,Neural Stem Cells ,Neurogenesis ,Neurons ,Protein Binding ,14-3-3 ,delta-catenin ,neurogenesis ,neuronal differentiation ,neuronal migration ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Neurology & Neurosurgery - Abstract
During brain development, neural progenitor cells proliferate and differentiate into neural precursors. These neural precursors migrate along the radial glial processes and localize at their final destination in the cortex. Numerous reports have revealed that 14-3-3 proteins are involved in many neuronal activities, although their functions in neurogenesis remain unclear. Here, using 14-3-3ε/ζ double knock-out mice, we found that 14-3-3 proteins are important for proliferation and differentiation of neural progenitor cells in the cortex, resulting in neuronal migration defects and seizures. 14-3-3 deficiency resulted in the increase of δ-catenin and the decrease of β-catenin and αN-catenin. 14-3-3 proteins regulated neuronal differentiation into neurons via direct interactions with phosphorylated δ-catenin to promote F-actin formation through a catenin/Rho GTPase/Limk1/cofilin signaling pathway. Conversely, neuronal migration defects seen in the double knock-out mice were restored by phosphomimic Ndel1 mutants, but not δ-catenin. Our findings provide new evidence that 14-3-3 proteins play important roles in neurogenesis and neuronal migration via the regulation of distinct signaling cascades.
- Published
- 2014
35. Lhx6 Directly Regulates Arx and CXCR7 to Determine Cortical Interneuron Fate and Laminar Position
- Author
-
Vogt, Daniel, Hunt, Robert F, Mandal, Shyamali, Sandberg, Magnus, Silberberg, Shanni N, Nagasawa, Takashi, Yang, Zhengang, Baraban, Scott C, and Rubenstein, John LR
- Subjects
Biomedical and Clinical Sciences ,Neurosciences ,Biotechnology ,Genetics ,1.1 Normal biological development and functioning ,Underpinning research ,Action Potentials ,Age Factors ,Animals ,Cell Movement ,Cerebral Cortex ,Chemokine CXCL1 ,Embryo ,Mammalian ,Gene Expression Regulation ,Developmental ,HEK293 Cells ,Homeodomain Proteins ,Humans ,In Vitro Techniques ,Interneurons ,LIM-Homeodomain Proteins ,Luminescent Proteins ,Mice ,Mice ,Transgenic ,Nerve Tissue Proteins ,Receptors ,CXCR ,Stem Cell Transplantation ,Stem Cells ,Transcription Factors ,Psychology ,Cognitive Sciences ,Neurology & Neurosurgery ,Biological psychology - Abstract
Cortical GABAergic interneurons have essential roles for information processing and their dysfunction is implicated in neuropsychiatric disorders. Transcriptional codes are elucidating mechanisms of interneuron specification in the MGE (a subcortical progenitor zone), which regulate their migration, integration, and function within cortical circuitry. Lhx6, a LIM-homeodomain transcription factor, is essential for specification of MGE-derived somatostatin and parvalbumin interneurons. Here, we demonstrate that some Lhx6⁻/⁻ MGE cells acquire a CGE-like fate. Using an in vivo MGE complementation/transplantation assay, we show that Lhx6-regulated genes Arx and CXCR7 rescue divergent aspects of Lhx6⁻/⁻ cell-fate and laminar mutant phenotypes and provide insight into a neonatal role for CXCR7 in MGE-derived interneuron lamination. Finally, Lhx6 directly binds in vivo to an Arx enhancer and to an intronic CXCR7 enhancer that remains active in mature interneurons. These data define the molecular identity of Lhx6 mutants and introduce technologies to test mechanisms in GABAergic interneuron differentiation.
- Published
- 2014
36. Neocortical integration of transplanted GABA progenitor cells from wild type and GABAB receptor knockout mouse donors
- Author
-
Sebe, Joy Y, Looke-Stewart, Elizabeth, Dinday, Matthew T, Alvarez-Buylla, Arturo, and Baraban, Scott C
- Subjects
Biological Psychology ,Biomedical and Clinical Sciences ,Neurosciences ,Psychology ,Regenerative Medicine ,Brain Disorders ,Transplantation ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research ,Neurological ,Animals ,Cell Count ,Embryonic Stem Cells ,GABAergic Neurons ,Interneurons ,Median Eminence ,Mice ,Knockout ,Neocortex ,Neural Stem Cells ,Receptors ,GABA-B ,Interneuron ,GABA ,Laminar ,Cognitive Sciences ,Biochemistry and cell biology ,Biological psychology - Abstract
Most cortical interneurons originate in a region of the embryonic subpallium called the medial ganglionic eminence (MGE). When MGE cells are transplanted into cerebral cortex, these progenitors migrate extensively and differentiate into functional inhibitory neurons. Although MGE progenitors have therapeutic potential following transplantation, it is unknown precisely how these cells distribute within neocortical lamina of the recipient brain. Here we transplanted mouse embryonic day 12.5 MGE progenitors into postnatal neocortex and evaluated laminar distribution of interneuron subtypes using double- and triple-label immunohistochemistry. Studies were performed using wild type (WT) or donor mice lacking a metabotropic GABA(B) receptor subunit (GABA(B1)R KO). MGE-derived neurons from WT and GABA(B1)R KO mice preferentially and densely distributed in neocortical layers 2/3, 5 and 6. As expected, MGE-derived neurons differentiated into parvalbumin+ and somatostatin+ interneurons within these neocortical lamina. Our findings provide insights into the anatomical integration of MGE-derived interneurons following transplantation.
- Published
- 2014
37. Olig1 Function Is Required to Repress Dlx1/2 and Interneuron Production in Mammalian Brain
- Author
-
Silbereis, John C, Nobuta, Hiroko, Tsai, Hui-Hsin, Heine, Vivi M, McKinsey, Gabriel L, Meijer, Dimphna H, Howard, MacKenzie A, Petryniak, Magda A, Potter, Gregory B, Alberta, John A, Baraban, Scott C, Stiles, Charles D, Rubenstein, John LR, and Rowitch, David H
- Subjects
Biological Psychology ,Biomedical and Clinical Sciences ,Psychology ,Stem Cell Research ,Mental Health ,Brain Disorders ,Genetics ,Neurosciences ,Stem Cell Research - Nonembryonic - Non-Human ,1.1 Normal biological development and functioning ,Underpinning research ,Neurological ,Action Potentials ,Age Factors ,Animals ,Basic Helix-Loop-Helix Transcription Factors ,Brain ,Cell Count ,DNA-Binding Proteins ,Embryo ,Mammalian ,Gene Expression Regulation ,Developmental ,Glutamate Decarboxylase ,Homeodomain Proteins ,Interneurons ,Mice ,Mice ,Inbred C57BL ,Mice ,Transgenic ,Mutation ,Nerve Tissue Proteins ,Neuropeptides ,Organ Culture Techniques ,Patch-Clamp Techniques ,Synapses ,Transcription Factors ,Cognitive Sciences ,Neurology & Neurosurgery ,Biological psychology - Abstract
Abnormal GABAergic interneuron density, and imbalance of excitatory versus inhibitory tone, is thought to result in epilepsy, neurodevelopmental disorders, and psychiatric disease. Recent studies indicate that interneuron cortical density is determined primarily by the size of the precursor pool in the embryonic telencephalon. However, factors essential for regulating interneuron allocation from telencephalic multipotent precursors are poorly understood. Here we report that Olig1 represses production of GABAergic interneurons throughout the mouse brain. Olig1 deletion in mutant mice results in ectopic expression and upregulation of Dlx1/2 genes in the ventral medial ganglionic eminences and adjacent regions of the septum, resulting in an ∼30% increase in adult cortical interneuron numbers. We show that Olig1 directly represses the Dlx1/2 I12b intergenic enhancer and that Dlx1/2 functions genetically downstream of Olig1. These findings establish Olig1 as an essential repressor of Dlx1/2 and interneuron production in developing mammalian brain.
- Published
- 2014
38. Bidirectional homeostatic plasticity induced by interneuron cell death and transplantation in vivo
- Author
-
Howard, MacKenzie Allen, Rubenstein, John LR, and Baraban, Scott C
- Subjects
Neurosciences ,Transplantation ,Neurological ,Animals ,Cell Death ,Cell Transplantation ,Electrophysiology ,Excitatory Postsynaptic Potentials ,GABAergic Neurons ,Gene Silencing ,Green Fluorescent Proteins ,Hippocampus ,Homeodomain Proteins ,Homeostasis ,Immunohistochemistry ,Interneurons ,Long-Term Potentiation ,Male ,Mice ,Neural Stem Cells ,Neuronal Plasticity ,Neurons ,Oscillometry ,Synapses ,Synaptic Transmission ,Transcription Factors ,gamma-Aminobutyric Acid ,excitatory/inhibitory balance ,gamma frequency oscillations ,LTP ,neural transplantation - Abstract
Chronic changes in excitability and activity can induce homeostatic plasticity. These perturbations may be associated with neurological disorders, particularly those involving loss or dysfunction of GABA interneurons. In distal-less homeobox 1 (Dlx1(-/-)) mice with late-onset interneuron loss and reduced inhibition, we observed both excitatory synaptic silencing and decreased intrinsic neuronal excitability. These homeostatic changes do not fully restore normal circuit function, because synaptic silencing results in enhanced potential for long-term potentiation and abnormal gamma oscillations. Transplanting medial ganglionic eminence interneuron progenitors to introduce new GABAergic interneurons, we demonstrate restoration of hippocampal function. Specifically, miniature excitatory postsynaptic currents, input resistance, hippocampal long-term potentiation, and gamma oscillations are all normalized. Thus, in vivo homeostatic plasticity is a highly dynamic and bidirectional process that responds to changes in inhibition.
- Published
- 2014
39. What New Modeling Approaches Will Help Us Identify Promising Drug Treatments?
- Author
-
Baraban, Scott C and Löscher, Wolfgang
- Subjects
Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Neurosciences ,Biotechnology ,Neurodegenerative ,Brain Disorders ,Epilepsy ,5.1 Pharmaceuticals ,Development of treatments and therapeutic interventions ,Neurological ,Animals ,Anticonvulsants ,Dogs ,Drug Discovery ,Humans ,Models ,Biological ,Rodentia ,Zebrafish ,Epileptic dogs ,Epileptic rodents ,Pharmacoresistant epilepsy ,Antiepileptic drugs ,Epilepsy syndromes ,Medical and Health Sciences ,General & Internal Medicine ,Biological sciences ,Biomedical and clinical sciences - Abstract
Despite the development of numerous novel antiepileptic drugs (AEDs) in recent years, several unmet clinical needs remain, including resistance to AEDs in about 30 % of patients with epilepsy, adverse effects of AEDs that can reduce quality of life, and the lack of treatments that can prevent development of epilepsy in patients at risk. Animal models of seizures and epilepsy have been instrumental in the discovery and preclinical development of novel AEDs, but obviously the previously used models have failed to identify drugs that address unmet medical needs. Thus, we urgently need fresh ideas for improving preclinical AED development. In this review, a number of promising models will be described, including the use of simple vertebrates such as zebrafish (Danio rerio), large animal models such as the dog and newly characterized rodent models of pharmacoresistant epilepsy. While these strategies, like any animal model approach also have their limitations, they offer hope that new more effective AEDs will be identified in the coming years.
- Published
- 2014
40. GABA progenitors grafted into the adult epileptic brain control seizures and abnormal behavior.
- Author
-
Hunt, Robert F, Girskis, Kelly M, Rubenstein, John L, Alvarez-Buylla, Arturo, and Baraban, Scott C
- Subjects
Telencephalon ,Interneurons ,Animals ,Mice ,Epilepsy ,Epilepsy ,Temporal Lobe ,Disease Models ,Animal ,Electroencephalography ,Stem Cell Transplantation ,Behavior ,Animal ,GABAergic Neurons ,Stem Cell Research ,Neurosciences ,Brain Disorders ,Stem Cell Research - Nonembryonic - Non-Human ,Neurodegenerative ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Psychology ,Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Impaired GABA-mediated neurotransmission has been implicated in many neurologic diseases, including epilepsy, intellectual disability and psychiatric disorders. We found that inhibitory neuron transplantation into the hippocampus of adult mice with confirmed epilepsy at the time of grafting markedly reduced the occurrence of electrographic seizures and restored behavioral deficits in spatial learning, hyperactivity and the aggressive response to handling. In the recipient brain, GABA progenitors migrated up to 1,500 μm from the injection site, expressed genes and proteins characteristic for interneurons, differentiated into functional inhibitory neurons and received excitatory synaptic input. In contrast with hippocampus, cell grafts into basolateral amygdala rescued the hyperactivity deficit, but did not alter seizure activity or other abnormal behaviors. Our results highlight a critical role for interneurons in epilepsy and suggest that interneuron cell transplantation is a powerful approach to halting seizures and rescuing accompanying deficits in severely epileptic mice.
- Published
- 2013
41. Use of "MGE enhancers" for labeling and selection of embryonic stem cell-derived medial ganglionic eminence (MGE) progenitors and neurons.
- Author
-
Chen, Ying-Jiun J, Vogt, Daniel, Wang, Yanling, Visel, Axel, Silberberg, Shanni N, Nicholas, Cory R, Danjo, Teruko, Pollack, Joshua L, Pennacchio, Len A, Anderson, Stewart, Sasai, Yoshiki, Baraban, Scott C, Kriegstein, Arnold R, Alvarez-Buylla, Arturo, and Rubenstein, John LR
- Subjects
Prosencephalon ,Cells ,Cultured ,Animals ,Mice ,Transgenic ,Humans ,Mice ,Luminescent Proteins ,Flow Cytometry ,Cell Separation ,Staining and Labeling ,Transduction ,Genetic ,Cell Differentiation ,Gene Expression ,Gene Expression Regulation ,Developmental ,Female ,Male ,Enhancer Elements ,Genetic ,Promoter Regions ,Genetic ,HEK293 Cells ,Embryoid Bodies ,Neural Stem Cells ,Transcriptome ,GABAergic Neurons ,Biomarkers ,Stem Cell Research - Embryonic - Non-Human ,Brain Disorders ,Stem Cell Research ,Stem Cell Research - Nonembryonic - Non-Human ,Regenerative Medicine ,Transplantation ,Neurosciences ,Neurological ,General Science & Technology - Abstract
The medial ganglionic eminence (MGE) is an embryonic forebrain structure that generates the majority of cortical interneurons. MGE transplantation into specific regions of the postnatal central nervous system modifies circuit function and improves deficits in mouse models of epilepsy, Parkinson's disease, pain, and phencyclidine-induced cognitive deficits. Herein, we describe approaches to generate MGE-like progenitor cells from mouse embryonic stem (ES) cells. Using a modified embryoid body method, we provided gene expression evidence that mouse ES-derived Lhx6(+) cells closely resemble immature interneurons generated from authentic MGE-derived Lhx6(+) cells. We hypothesized that enhancers that are active in the mouse MGE would be useful tools in detecting when ES cells differentiate into MGE cells. Here we demonstrate the utility of enhancer elements [422 (DlxI12b), Lhx6, 692, 1056, and 1538] as tools to mark MGE-like cells in ES cell differentiation experiments. We found that enhancers DlxI12b, 692, and 1538 are active in Lhx6-GFP(+) cells, while enhancer 1056 is active in Olig2(+) cells. These data demonstrate unique techniques to follow and purify MGE-like derivatives from ES cells, including GABAergic cortical interneurons and oligodendrocytes, for use in stem cell-based therapeutic assays and treatments.
- Published
- 2013
42. Impaired neural development in a zebrafish model for Lowe syndrome
- Author
-
Ramirez, Irene Barinaga-Rementeria, Pietka, Grzegorz, Jones, David R, Divecha, Nullin, Alia, A, Baraban, Scott C, Hurlstone, Adam FL, and Lowe, Martin
- Subjects
Congenital Structural Anomalies ,Neurosciences ,Brain Disorders ,Pediatric ,Rare Diseases ,Underpinning research ,1.1 Normal biological development and functioning ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Animals ,Brain ,Cell Survival ,Clathrin ,Disease Models ,Animal ,Embryo ,Nonmammalian ,Embryonic Development ,Endosomes ,Gene Expression Profiling ,Golgi Apparatus ,Hot Temperature ,Oculocerebrorenal Syndrome ,Phosphatidylinositol 4 ,5-Diphosphate ,Phosphoric Monoester Hydrolases ,Protein Splicing ,Proto-Oncogene Proteins c-akt ,Seizures ,Signal Transduction ,Zebrafish ,Zebrafish Proteins ,Biological Sciences ,Medical and Health Sciences ,Genetics & Heredity - Abstract
Lowe syndrome, which is characterized by defects in the central nervous system, eyes and kidneys, is caused by mutation of the phosphoinositide 5-phosphatase OCRL1. The mechanisms by which loss of OCRL1 leads to the phenotypic manifestations of Lowe syndrome are currently unclear, in part, owing to the lack of an animal model that recapitulates the disease phenotype. Here, we describe a zebrafish model for Lowe syndrome using stable and transient suppression of OCRL1 expression. Deficiency of OCRL1, which is enriched in the brain, leads to neurological defects similar to those reported in Lowe syndrome patients, namely increased susceptibility to heat-induced seizures and cystic brain lesions. In OCRL1-deficient embryos, Akt signalling is reduced and there is both increased apoptosis and reduced proliferation, most strikingly in the neural tissue. Rescue experiments indicate that catalytic activity and binding to the vesicle coat protein clathrin are essential for OCRL1 function in these processes. Our results indicate a novel role for OCRL1 in neural development, and support a model whereby dysregulation of phosphoinositide metabolism and clathrin-mediated membrane traffic leads to the neurological symptoms of Lowe syndrome.
- Published
- 2012
43. Cortical inhibition modified by embryonic neural precursors grafted into the postnatal brain
- Author
-
Alvarez-Dolado, Manuel, Calcagnotto, Maria Elisa, Karkar, Kameel M, Southwell, Derek G, Jones-Davis, Dorothy M, Estrada, Rosanne C, Rubenstein, John L R, Alvarez-Buylla, Arturo, and Baraban, Scott C
- Subjects
progenitor cells ,GFP ,electrophysiology ,whole-cell recording ,MGE ,interneuron ,GABA - Abstract
Embryonic medial ganglionic eminence (MGE) cells transplanted into the adult brain can disperse, migrate, and differentiate to neurons expressing GABA, the primary inhibitory neurotransmitter. It has been hypothesized that grafted MGE precursors could have important therapeutic applications increasing local inhibition, but there is no evidence that MGE cells can modify neural circuits when grafted into the postnatal brain. Here we demonstrate that MGE cells grafted into one location of the neonatal rodent brain migrate widely into cortex. Grafted MGE-derived cells differentiate into mature cortical interneurons; the majority of these new interneurons express GABA. Based on their morphology and expression of somatostatin, neuropeptide Y, parvalbumin, or calretinin, we infer that graft-derived cells integrate into local circuits and function as GABA-producing inhibitory cells. Whole-cell current-clamp recordings obtained from MGE-derived cells indicate firing properties typical of mature interneurons. Moreover, patch-clamp recordings of IPSCs on pyramidal neurons in the host brain, 30 and 60 d after transplantation, indicated a significant increase in GABA-mediated synaptic inhibition in regions containing transplanted MGE cells. In contrast, synaptic excitation is not altered in the host brain. Grafted MGE cells, therefore, can be used to modify neural circuits and selectively increase local inhibition. These findings could have important implications for reparative cell therapies for brain disorders.
- Published
- 2006
44. Role of the Y5 Neuropeptide Y Receptor in Limbic Seizures
- Author
-
Marsh, Donald J., Baraban, Scott C., Hollopeter, Gunther, and Palmiter, Richard D.
- Published
- 1999
45. Cell therapy for neurological disorders: Progress towards an embryonic medial ganglionic eminence progenitor-based treatment
- Author
-
Righes Marafiga, Joseane, primary and Baraban, Scott C., additional
- Published
- 2023
- Full Text
- View/download PDF
46. What New Modeling Approaches Will Help Us Identify Promising Drug Treatments?
- Author
-
Baraban, Scott C., Löscher, Wolfgang, Cohen, Irun R., Series editor, Lajtha, N.S. Abel, Series editor, Lambris, John D., Series editor, Paoletti, Rodolfo, Series editor, Scharfman, Helen E., editor, and Buckmaster, Paul S., editor
- Published
- 2014
- Full Text
- View/download PDF
47. GABAB receptors in maintenance of neocortical circuit function
- Author
-
Sebe, Joy Y., Looke-Stewart, Elizabeth, and Baraban, Scott C.
- Published
- 2014
- Full Text
- View/download PDF
48. A novel zebrafish model of hyperthermia-induced seizures reveals a role for TRPV4 channels and NMDA-type glutamate receptors
- Author
-
Hunt, Robert F., Hortopan, Gabriela A., Gillespie, Anna, and Baraban, Scott C.
- Published
- 2012
- Full Text
- View/download PDF
49. Reduction of Seizures by Transplantation of Cortical GABAergic Interneuron Precursors into Kv1.1 Mutant Mice
- Author
-
Baraban, Scott C., Southwell, Derek G., Estrada, Rosanne C., Jones, Daniel L., Sebe, Joy Y., Alfaro-Cervello, Clara, García-Verdugo, Jose M., Rubenstein, John L. R., Alvarez-Buylla, Arturo, and Jan, Lily Y.
- Published
- 2009
- Full Text
- View/download PDF
50. Clemizole and Trazodone are Effective Antiseizure Treatments in a Zebrafish Model of STXBP1 Disorder
- Author
-
Moog, Maia, primary and Baraban, Scott C, additional
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.