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4 results on '"Barau, Joan G."'

1. A potential role for an extracellular methanol oxidase secreted by Moniliophthora perniciosa in Witches’ broom disease in cacao

Author

de Oliveira, Bruno V., primary, Teixeira, Gleidson S., additional, Reis, Osvaldo, additional, Barau, Joan G., additional, Teixeira, Paulo José P.L., additional, do Rio, Maria Carolina S., additional, Domingues, Romênia R., additional, Meinhardt, Lyndel W., additional, Paes Leme, Adriana F., additional, Rincones, Johana, additional, and Pereira, Gonçalo A.G., additional

Published
2012
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3. Differential Gene Expression Between the Biotrophic-Like and Saprotrophic Mycelia of the Witches' Broom Pathogen Moniliophthora perniciosa

Author

Rincones, Johana, primary, Scarpari, Leandra M., additional, Carazzolle, Marcelo F., additional, Mondego, Jorge M. C., additional, Formighieri, Eduardo F., additional, Barau, Joan G., additional, Costa, Gustavo G. L., additional, Carraro, Dirce M., additional, Brentani, Helena P., additional, Vilas-Boas, Laurival A., additional, de Oliveira, Bruno V., additional, Sabha, Maricene, additional, Dias, Robson, additional, Cascardo, Júlio M., additional, Azevedo, Ricardo A., additional, Meinhardt, Lyndel W., additional, and Pereira, Gonçalo A. G., additional

Published
2008
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4. The glyceraldehyde-3-phosphate dehydrogenase gene of Moniliophthoraperniciosa, the causal agent of witches' broom disease of Theobroma cacao.

Author

Lima JO, Pereira JF, Rincones J, Barau JG, Araújo EF, Pereira GA, and Queiroz MV

Abstract

This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were conserved in the GAPDH genes of other basidiomycete species. The cis-elements in the promoter region of the MpGAPDH gene were similar to those of other basidiomycetes. Likewise, the MpGAPDH gene encoded a putative 339 amino acid protein that shared significant sequence similarity with other GAPDH proteins in fungi, plants, and metazoans. Phylogenetic analyses clustered the MPGAPDH protein with other homobasidiomycete fungi of the family Tricholomataceae. Expression analysis of the MpGAPDH gene by real-time PCR showed that this gene was more expressed (~1.3X) in the saprotrophic stage of this hemibiotrophic plant pathogen than in the biotrophic stage when grown in cacao extracts.

Published
2009
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