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11. Determination of Perfluorinated and Polyfluorinated Alkyl Substances (PFASs) in PM 10 Samples: Analytical Method, Seasonal Trends, and Implications for Urban Air Quality in the City of Terni (Central Italy).

Author

Moretti, Simone, Castellini, Silvia, Barola, Carolina, Bucaletti, Elisabetta, Petroselli, Chiara, Selvaggi, Roberta, Galletti, Mara, Cappelletti, David, and Galarini, Roberta

Subjects
FLUOROALKYL compounds, AIR pollutants, PARTICULATE matter, PERFLUOROOCTANOIC acid, PERFLUOROOCTANE sulfonate, AIR quality, SEASONS, SAMPLING methods
Abstract

A quantitative analytical method for PFAS determination in airborne particulate matter (PM) has been developed using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS), allowing for the determination of 33 compounds. The procedure was applied to ambient PM10 with limits of quantification for PFAS in the fg m−3 range. PM10 samples collected during a year-long campaign conducted in an urban site in Umbria (Central Italy) have been characterized for their PFAS content. Among the seven detected PFASs, perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) were the most abundant compounds. Furthermore, this work allowed us to obtain the first seasonal trend of airborne PFASs in Central Italy. Seasonal trend analysis shows that PFAS concentration in the atmosphere peaks in summertime. A comparison with trends of other relevant primary and secondary air pollutants determined at the sampling site suggests a secondary nature of the observed PFAS, which are formed in the atmosphere directly from gaseous precursors and can contribute to worsen the urban air quality in summertime. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Untargeted Screening of Per- and Polyfluoroalkyl Substances (PFASs) in Airborne Particulate of Three Italian E-Waste Recycling Facilities

Author

Barola, Carolina, primary, Bucaletti, Elisabetta, additional, Moretti, Simone, additional, Buiarelli, Francesca, additional, Simonetti, Giulia, additional, Lucarelli, Franco, additional, Goracci, Laura, additional, Lorenzetti, Stefano, additional, Di Filippo, Patrizia, additional, Pomata, Donatella, additional, Riccardi, Carmela, additional, and Galarini, Roberta, additional

Published
2023
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16. Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Author

van de Veerdonk, Frank L., Renga, Giorgia, Pariano, Marilena, Belle, Marina M., Servillo, Giuseppe, Fallarino, Francesca, De Luca, Antonella, Iannitti, Rossana G., Piobbico, Danilo, Gargaro, Marco, Manni, Giorgia, D'Onofrio, Fiorella, Stincardini, Claudia, Sforna, Luigi, Borghi, Monica, Castelli, Marilena, Pieroni, Stefania, Oikonomou, Vasileios, Villella, Valeria R., Puccetti, Matteo, Giovagnoli, Stefano, Galarini, Roberta, Barola, Carolina, Maiuri, Luigi, Fazia, Maria Agnese Della, Cellini, Barbara, Talesa, Vincenzo Nicola, Dinarello, Charles A., Costantini, Claudio, and Romani, Luigina

Subjects
Homeostasis -- Research, Pharmacology, Experimental, Autophagy (Cytology) -- Physiological aspects -- Health aspects, Mitochondria -- Health aspects -- Physiological aspects, Cellular proteins -- Physiological aspects -- Health aspects, Oxidation-reduction reaction -- Research, Health care industry
Abstract

Autophagy selectively degrades aggregation-prone misfolded proteins caused by defective cellular proteostasis. However, the complexity of autophagy may prevent the full appreciation of how its modulation could be used as a therapeutic strategy in disease management. Here, we define a molecular pathway through which recombinant IL-1 receptor antagonist (IL1Ra, anakinra) affects cellular proteostasis independently from the IL-1 receptor (IL-1R1). Anakinra promoted [H.sub.2][O.sub.2]-driven autophagy through a xenobiotic sensing pathway involving the aryl hydrocarbon receptor that, activated through the indoleamine 2,3-dioxygenase 1-kynurenine pathway, transcriptionally activated NADPH oxidase 4 independent of the IL- 1R1. By coupling the mitochondrial redox balance to autophagy, anakinra improved the dysregulated proteostasis network in murine and human cystic fibrosis. We anticipate that anakinra may represent a therapeutic option in addition to its IL- 1R1dependent antiinflammatory properties by acting at the intersection of mitochondrial oxidative stress and autophagy with the capacity to restore conditions in which defective proteostasis leads to human disease., Introduction The lung is equipped with a robust proteostasis network for handling protein folding, misfolding, unfolding, and degradation in response to mechanical and environmental stress (1). Consistent with the emerging [...]

Published
2022
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21. Aryl Hydrocarbon Receptor Agonism Antagonizes the Hypoxia-driven Inflammation in Cystic Fibrosis

Author

Pariano, Marilena, primary, Puccetti, Matteo, additional, Stincardini, Claudia, additional, Napolioni, Valerio, additional, Gatticchi, Leonardo, additional, Galarini, Roberta, additional, Renga, Giorgia, additional, Barola, Carolina, additional, Bellet, Marina M, additional, D'Onofrio, Fiorella, additional, Nunzi, Emilia, additional, Bartoli, Andrea, additional, Antognelli, Cinzia, additional, Cariani, Lisa, additional, Russo, Maria, additional, Porcaro, Luigi, additional, Colombo, Carla, additional, Majo, Fabio, additional, Lucidi, Vincenzina, additional, Montemitro, Enza, additional, Fiscarelli, Ersilia, additional, Ellemunter, Helmut, additional, Lass-Flörl, Cornelia, additional, Ricci, Maurizio, additional, Costantini, Claudio, additional, Giovagnoli, Stefano, additional, and Romani, Luigina, additional

Published
2022
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22. Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Author

van de Veerdonk, Frank L, Renga, Giorgia, Pariano, Marilena, Bellet, Marina M, Servillo, Giuseppe, Fallarino, Francesca, De Luca, Antonella, Iannitti, Rossana G, Piobbico, Danilo, Gargaro, Marco, Manni, Giorgia, D'Onofrio, Fiorella, Stincardini, Claudia, Sforna, Luigi, Borghi, Monica, Castelli, Marilena, Pieroni, Stefania, Oikonomou, Vasileios, Villella, Valeria R, Puccetti, Matteo, Giovagnoli, Stefano, Galarini, Roberta, Barola, Carolina, Maiuri, Luigi, Della Fazia, Maria Agnese, Cellini, Barbara, Talesa, Vincenzo Nicola, Dinarello, Charles A, Costantini, Claudio, Romani, Luigina, van de Veerdonk, Frank L, Renga, Giorgia, Pariano, Marilena, Bellet, Marina M, Servillo, Giuseppe, Fallarino, Francesca, De Luca, Antonella, Iannitti, Rossana G, Piobbico, Danilo, Gargaro, Marco, Manni, Giorgia, D'Onofrio, Fiorella, Stincardini, Claudia, Sforna, Luigi, Borghi, Monica, Castelli, Marilena, Pieroni, Stefania, Oikonomou, Vasileio, Villella, Valeria R, Puccetti, Matteo, Giovagnoli, Stefano, Galarini, Roberta, Barola, Carolina, Maiuri, Luigi, Della Fazia, Maria Agnese, Cellini, Barbara, Talesa, Vincenzo Nicola, Dinarello, Charles A, Costantini, Claudio, and Romani, Luigina

Subjects
Fungal infection, Male, medicine.drug_class, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], medicine.disease_cause, Mice, Fungal infections, All institutes and research themes of the Radboud University Medical Center, medicine, Autophagy, Animals, Receptor, Mice, Knockout, Inflammation, Anakinra, Infectious disease, NADPH oxidase, biology, Chemistry, General Medicine, Aryl hydrocarbon receptor, Receptor antagonist, Cell biology, Mitochondria, Interleukin 1 Receptor Antagonist Protein, Oxidative Stress, Proteostasis, biology.protein, Female, Oxidation-Reduction, Oxidative stress, medicine.drug, Research Article
Abstract

Autophagy selectively degrades aggregation-prone misfolded proteins caused by defective cellular proteostasis. However, the complexity of autophagy may prevent the full appreciation of how its modulation could be used as a therapeutic strategy in disease management. Here we define a molecular pathway through which recombinant interleukin-1 receptor antagonist (IL-1Ra, anakinra) affects cellular proteostasis independently from the IL-1 receptor (IL-1R1). Anakinra promoted H2O2-driven autophagy through a xenobiotic sensing pathway involving the aryl hydrocarbon receptor that, activated through the indoleamine 2,3-dioxygenase 1-kynurenine pathway, transcriptionally activates NADPH Oxidase 4 independent of the IL-1R1. By coupling the mitochondrial redox balance to autophagy, anakinra improved the dysregulated proteostasis network in murine and human cystic fibrosis. We anticipate that anakinra may represent a therapeutic option in addition to its IL-1R1 dependent anti-inflammatory properties by acting at the intersection of mitochondrial oxidative stress and autophagy with the capacity to restore conditions in which defective proteostasis leads to human disease.

Published
2022

24. Aryl Hydrocarbon Receptor Agonism Antagonizes the Hypoxia-driven Inflammation in Cystic Fibrosis.

Author

Pariano, Marilena, Puccetti, Matteo, Stincardini, Claudia, Napolioni, Valerio, Gatticchi, Leonardo, Galarini, Roberta, Renga, Giorgia, Barola, Carolina, Bellet, Marina M., D'Onofrio, Fiorella, Nunzi, Emilia, Bartoli, Andrea, Antognelli, Cinzia, Cariani, Lisa, Russo, Maria, Porcaro, Luigi, Colombo, Carla, Majo, Fabio, Lucidi, Vincenzina, and Montemitro, Enza

Subjects
ARYL hydrocarbon receptors, CYSTIC fibrosis, LUNGS, HYPOXIA-inducible factor 1, HYPOXIA-inducible factors, ASPERGILLOSIS, ASPERGILLUS fumigatus
Abstract

Hypoxia contributes to the exaggerated yet ineffective airway inflammation that fails to oppose infections in cystic fibrosis (CF). However, the potential for impairment of essential immune functions by HIF-1α (hypoxia-inducible factor 1α) inhibition demands a better comprehension of downstream hypoxia-dependent pathways that are amenable for manipulation. We assessed here whether hypoxia may interfere with the activity of AhR (aryl hydrocarbon receptor), a versatile environmental sensor highly expressed in the lungs, where it plays a homeostatic role. We used murine models of Aspergillus fumigatus infection in vivo and human cells in vitro to define the functional role of AhR in CF, evaluate the impact of hypoxia on AhR expression and activity, and assess whether AhR agonism may antagonize hypoxia-driven inflammation. We demonstrated that there is an important interferential cross-talk between the AhR and HIF-1α signaling pathways in murine and human CF, in that HIF-1α induction squelched the normal AhR response through an impaired formation of the AhR:ARNT (aryl hydrocarbon receptor nuclear translocator)/HIF-1β heterodimer. However, functional studies and analysis of the AhR genetic variability in patients with CF proved that AhR agonism could prevent hypoxia-driven inflammation, restore immune homeostasis, and improve lung function. This study emphasizes the contribution of environmental factors, such as infections, in CF disease progression and suggests the exploitation of hypoxia:xenobiotic receptor cross-talk for antiinflammatory therapy in CF. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Alpha-Tocopherol Metabolites (The Vitamin E Metabolome) and Their Interindividual Variability during Supplementation

Author

Bartolini, Desirée, primary, Marinelli, Rita, additional, Giusepponi, Danilo, additional, Galarini, Roberta, additional, Barola, Carolina, additional, Stabile, Anna Maria, additional, Sebastiani, Bartolomeo, additional, Paoletti, Fabiola, additional, Betti, Michele, additional, Rende, Mario, additional, and Galli, Francesco, additional

Published
2021
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29. LC-MS/MS assay for the simultaneous determination of tocopherols, polyunsaturated fatty acids and their metabolites in human plasma and serum

Author

Giusepponi, Danilo, primary, Galarini, Roberta, additional, Barola, Carolina, additional, Torquato, Pierangelo, additional, Bartolini, Desirée, additional, Moretti, Simone, additional, Saluti, Giorgio, additional, Gioiello, Antimo, additional, Libetta, Carmelo, additional, and Galli, Francesco, additional

Published
2019
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30. Nonalcoholic fatty liver disease impairs the cytochrome P-450-dependent metabolism of α-tocopherol (vitamin E)

Author

Bartolini, Desirée, primary, Torquato, Pierangelo, additional, Barola, Carolina, additional, Russo, Angelo, additional, Rychlicki, Chiara, additional, Giusepponi, Danilo, additional, Bellezza, Guido, additional, Sidoni, Angelo, additional, Galarini, Roberta, additional, Svegliati-Baroni, Gianluca, additional, and Galli, Francesco, additional

Published
2017
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31. Analytical and molecular insights in the cytochrome P450 metabolism of vitamin E and lipotoxicity mechanisms of non-alcoholic fatty liver

Author

Rychlicki Chiara, Galarini Roberta, Torquato Pierangelo, Pierantonelli Irene, Svegliati-Baroni Gianluca, Galli Francesco, Ripa Orsola, Barola Carolina, and Desirée Bartolini

Subjects
0301 basic medicine, biology, Chemistry, Vitamin E, medicine.medical_treatment, Fatty liver, Cytochrome P450, Non alcoholic, Metabolism, medicine.disease, Biochemistry, 03 medical and health sciences, 030104 developmental biology, Lipotoxicity, Physiology (medical), medicine, biology.protein
Published
2016

35. Transfer of a Multiclass Method for over 60 Antibiotics in Food from High Resolution to Low Resolution Mass Spectrometry.

Author

Giusepponi, Danilo, Paoletti, Fabiola, Barola, Carolina, Moretti, Simone, Saluti, Giorgio, Ianni, Federica, Sardella, Roccaldo, Galarini, Roberta, Gentili, Alessandra, and Fanali, Chiara

Subjects
MASS spectrometry, ANTIBIOTICS, TANDEM mass spectrometry, LIQUID chromatography
Abstract

A multiclass method has been developed to screen and confirm a wide range of anti-microbial residues in muscle and milk, and validated using liquid-chromatography coupled to (low-resolution, LR) tandem mass spectrometry (LC-QqQ). Over sixty antibiotics, belonging to ten distinct families, were included in the method scope. The development process was rapidly concluded as a result of two previously implemented methods. This consisted of identical sample treatments, followed by liquid chromatography, and coupled with high-resolution (HR) mass spectrometry (LC-Q-Orbitrap). The validation study was performed in the range between 10–1500 μg·kg−1 for muscles and 2–333 μg·kg−1 for milk. The main performance characteristics were estimated and, then, compared to those previously obtained with HR technique. The validity of the method transfer was ascertained also through inter-laboratory studies. [ABSTRACT FROM AUTHOR]

Published
2019
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37. YI-11 - a-13'-OH is the main product of a-tocopherol metabolism and influences CYP4F2 and PPAR? gene expression in HepG2 human hepatocarcinoma cells.

Author

Torquato, Pierangelo, Bartolini, Desire, Giusepponi, Danilo, Saluti, Giorgio, Russo, Angelo, Barola, Carolina, Birringer, Marc, Galarini, Roberta, and Galli, Francesco

Subjects
*LIVER cancer, *CYTOCHROME P-450, *VITAMIN metabolism, *PEROXISOME proliferator-activated receptors, *VITAMIN E, *GENE expression, *GENETICS
Published
2016
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