76 results on '"Barona J"'
Search Results
2. Comparison of vildagliptin and acarbose monotherapy in patients with Type 2 diabetes: a 24-week, double-blind, randomized trial
- Author
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Pan, C., Yang, W., Barona, J. P., Niggli, M., Mohideen, P., Wang, Y., and Foley, J. E.
- Published
- 2008
3. Scorpion envenoming in two regions of Colombia: clinical, epidemiological and therapeutic aspects
- Author
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Otero, R., Navı́o, E., Céspedes, F.A., Núñez, M.J., Lozano, L., Moscoso, E.R., Matallana, C., Arsuza, N.B., Garcı́a, J., Fernández, D., Rodas, J.H., Rodrı́guez, O.J., Zuleta, J.E., Gómez, J.P., Saldarriaga, M., Quintana, J.C., Núñez, V., Cárdenas, S., Barona, J., Valderrama, R., Paz, N., Dı́az, A., Rodrı́guez, O.L., Martı́nez, M.D., Maturana, R., Beltrán, L.E., Mesa, M.B., Paniagua, J., Flórez, E., and Lourenço, W.R.
- Published
- 2004
- Full Text
- View/download PDF
4. Pustular generalized perforating granuloma annulare
- Author
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VILLEGAS, R. GAMO, BARONA, J. SOPENA, TAPIA, A. GUERRA, SANCHEZ, A. VERGARA, PERALTO, J. L. RODRÍGUEZ, and DÍez, L. IGLESIAS
- Published
- 2003
5. The Role of Serum Inhibitors of Erythroid Colony-Forming Cells in the Mechanism of the Anemia of Renal Insufficiency
- Author
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Fisher, James W., Ohno, Yasuhico, Barona, J., Martinez, Maria, Rege, Arvind B., Murphy, Martin J., Jr., editor, Peschle, Cesare, editor, Gordon, Albert S., editor, and Mirand, Edwin A., editor
- Published
- 1978
- Full Text
- View/download PDF
6. Nutrition and Health. The International Context During the Inter-war Crisis
- Author
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Barona, J. L., primary
- Published
- 2008
- Full Text
- View/download PDF
7. La antropología de Marsilio Ficino
- Author
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Larrea-Barona, J. (Jesús)
- Subjects
Inmortalidad del alma ,Pruebas ,Antopología ,Arte y Humanidades::Filosofía [Materias Investigacion] ,Marsilio Ficino - Published
- 1991
8. Neutralization of the edema-forming, defibrinating and coagulant effects of Bothrops asper venom by extracts of plants used by healers in Colombia
- Author
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Núñez, V., primary, Otero, R., additional, Barona, J., additional, Saldarriaga, M., additional, Osorio, R.G., additional, Fonnegra, R., additional, Jiménez, S.L., additional, Díaz, A., additional, and Quintana, J.C., additional
- Published
- 2004
- Full Text
- View/download PDF
9. Pustular generalized perforating granuloma annulare
- Author
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Gamo Villegas, R., primary, Sopena Barona, J., additional, Guerra Tapia, A., additional, Vergara Sanchez, A., additional, Rodriguez Peralto, J.L., additional, and Iglesias DIez, L., additional
- Published
- 2003
- Full Text
- View/download PDF
10. Acromelanosis
- Author
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Sopena Barona, J., primary, Gamo Villegas, R., additional, Guerra Tapia, A., additional, and Iglesias Díez, L., additional
- Published
- 2003
- Full Text
- View/download PDF
11. Snakebites and ethnobotany in the northwest region of Colombia
- Author
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Otero, R, primary, Núñez, V, additional, Barona, J, additional, Fonnegra, R, additional, Jiménez, S.L, additional, Osorio, R.G, additional, Saldarriaga, M, additional, and Dı́az, A, additional
- Published
- 2000
- Full Text
- View/download PDF
12. CASE REPORT Pustular generalized perforating granuloma annulare.
- Author
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Villegas, R. Gamo, Barona, J. Sopena, Tapia, A. Guerra, Sanchez, A. Vergara, Rodríguez Peralto, J. L., and Díez, L. Iglesias
- Subjects
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GRANULOMA , *MALIGNANT pustule , *NEUTROPHILS , *ACTINOMYCES - Abstract
We report a 84-year-old man with a 13-year history of recurrent generalized asymptomatic pustular lesions. Histology revealed areas of necrobiosis surrounded by palisading granulomas and transepidermal and follicular elimination of the necrobiotic material. A dense infiltrate of neutrophils was also found. Although 26% of patients with generalized perforating granuloma annulare have some yellow pustule-like papules, these correspond histologically to the yellow viscous necrobiotic material extruding through the epidermis and not to a real neutrophilic infiltrate. This is the first case report of perforating granuloma annulare with recurrent generalized pustular lesions with a dense infiltrate of neutrophils. [ABSTRACT FROM AUTHOR]
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- 2003
- Full Text
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13. Utilización del retroproyector en las clases de química del bachillerato internacional
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Guillén Barona, J.
- Published
- 1987
14. The urban penalty and the deterioration of the nutritional health of the population: Health and hygiene response | La urban penalty y el deterioro de la salud nutricional de la población: La respuesta higiénico-sanitaria
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Barona, J. L., Bernabeu-Mestre, J., and María Eugenia Galiana-Sánchez
15. Health and the war. Changing schemes and health conditions during the Spanish civil war
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Barona, J. L. and Enrique Perdiguero-Gil
16. Alogliptin after acute coronary syndrome in patients with type 2 diabetes
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White, W. B., Cannon, C. P., Heller, S. R., Nissen, S. E., Bergenstal, R. M., Bakris, G. L., Perez, A. T., Fleck, P. R., Mehta, C. R., Kupfer, S., Wilson, C., Cushman, W. C., Zannad, F., Aiub, J., Albisu, J., Alvarez, C., Astesiano, A., Barcudi, R., Bendersky, M., Bono, J., Bustos, B., Cartasegna, L., Caruso, O., Casabe, H., Castro, R., Colombo, H., Cuneo, C., Cura, F., Loredo, L., Dran, R., Fernandez, H., Garcia Pinna, J., Hrabar, A., Klyver Saleme, M., Luquez, H., Mackinnon, I., Maffei, L., Majul, C., Mallagray, M., Marino, J., Martinez, D., Martingano, R., Nul, D., Parody, M. L., Petrucci, J., Pieroni, M., Daniel Piskorz, Prado, A., Ramos, H., Resk, J., Rodriguez, M., Rojas, C., Sarjanovich, R., Sarries, A., Sessa, H., Silveiro, S., Sosa Liprandi, M. I., Tartaglione, J., Tonin, H., Vallejos, J., Vigo, S., Visco, V., Vita, N., Vogel, D., Vogelmann, O., Zaidman, C., Zangroniz, P., Colquhoun, D., Coverdale, S., Flecknoe-Brown, S., Hii, C. S., Roberts-Thomson, P., Drexel, H., Luger, A., Pieber, T., Cools, F., Ruige, J., Schoors, D., Vercammen, C., Wollaert, B., Alves Da Costa, F., Amodeo, C., Baggenstoss, R., Barbosa, E., Barroso Souza, W. K., Bassan, R., Borges, J. L., Botelho, R., Braile, M. C., Castello, H., Chrisman, C., Dos Santos, F., Faria Neto, J., Farsky, P., Fernandes Da Costa, A., Fraige Filho, F., Garbelini, B., Garcia, M. F., Garzon, P., Guimaraes, A. E., Herdy, A., Hernandes, M., Hilgemberg, S., Hissa, M., Jatene, J. A., Kormann, A., Leaes, P., Lima, F., Lisboa, H. R., Maia, L., Maia Da Silva, F., Maldonado Franco, D., Martin, J. F., Medeiros, A., Michalaros, Y., Miguel Leitao, A., Montenegro, S., Moraes Junior, J., Mota Gomes, M., Paiva, M. S., Precoma, D., Rabelo, A., Reis, G., Reis, H., Rossi, P., Saporito, W., Sarmento Leite, R., Silva, R. P., Silva Junior, D., Sousa, L., Sousa, A. C., Ueda, R., Vilas-Boas, F., Wainstein, M., Zago, A., Angelova, M., Apostolova, E., Daskalova, I., Delchev, A., Hristozov, K., Ilieva, M., Kovacheva, S., Lucheva, M., Temelkova, M., Toneva, A., Videva, V., Vuchkova, E., Bakbak, A., Carpentier, A., Chan, Y. K., Cheema, A., Chouinard, G., Conway, J., Dery, J. P., Dowell, A., Frechette, A., Jakubowski, M., Kelly, A., Ma, P., Maung, T. Z., Mehta, S., Parker, D., Pesant, Y., Polasek, P., Ransom, T., Syan, G., Vizel, S., Albornoz, F., Castro Galvez, P., Cobos, J. L., Conejeros, C., D Acuña Apablaza, M., Fajardo, G., Illanes Brochet, G., Lazcano, M. O., Pincetti, C., Potthoff, S., Raffo, C., Saavedra, V., Schnettler, M., Sepulveda, P., Stockins, B., Vejar, M., Accini, J. L., Cotes Aroca, C. H., Fernandez Ruiz, R. L., Orozco Linares, L. A., Vesga Angarita, B. E., Aganovic, I., Bagatin, J., Canecki-Varzic, S., Erzen, D. J., Knezevic, A., Maric, A., Milicevic, G., Popovic, Z., Rubes, J., Weiss, S. S., Dresslerova, I., Havelkova, J., Kucera, D., Machacek, J., Pumprla, J., May, O., Perrild, H., Aziz, M. A., El Badry, M., Hasanein, M., Airaksinen, J., Laine, M., Nyman, K., Vikman, S., Bonnet, J., Elbaz, M., Henry, P., Paillard, F., Petit, C., Tropeano, A. I., Behnke, T., Bornstein, S., Busch, K., Ebelt, H., Faghih, M., Fischer, H., Heuer, H., Paschke, R., Porner, T. C., Tangerding, G., Vöhringer, H. F., Adamson, K., Beatt, K., Bellary, S., Chapman, J., Cooke, A., Fisher, M., Gnudi, L., Jones, H., Kumar, S., Nagi, D., Oldroyd, K., Richardson, T., Robertson, D., Robinson, A., Saravanan, P., Viljoen, A., Wilding, J., Wilkinson, P., Wong, Y. K., Zoupas, C., Arango, J., Castellanos, J., Ceren Flores, C., Corona, V., Granados-Fuentes, A., Haase, F., Montenegro, P., Prado, J. H., Villalobos, R., Chow, F., Li, S. K., Li, J., Yan, P. Y., Yeung, V., Abel, T., Benedek, A., Dezso, E., Dudas, M., Édes, I., Fulop, G., Kovács, A., Lupkovics, G., Merkely, B., Nagy, A., Oroszlan, T., Palinkas, A., Papp, A., Patkay, J., Simon, E., Sitkei, E., Tabak, A., Tomcsányi, J., Abdullakutty, J., Abhyankar, A., Akalkotkar, U., Alexander, T., Arneja, J., Aslam, N., Babu, P. R., Babu, B. R., Banker, D., Bantwal, G., Bhimashankar, P. R., Calton, R. K., Chopda, M., Dande, A., Dani, S., Deshpande, N., Dhanwal, D., Dharmadhikari, A., Gadkari, M., Garg, N., Ghaisas, N., Goyal, N. K., Gupta, J. B., Jawahirani, A., Joseph, S., Kumar, R., Kumble, M., Mathavan, A., Mathur, A., Mohanan, P. P., Nair, A., Nair, T., Namjoshi, D., Pinto, R., Prakash, G., Purushotham, R., Raju, S., Ramachandran, P., Ramesh, S. S., Rao, B., Ravikishore, A., Reddy, G. R., Roy, S., Sadhu, N., Sastry, B. K., Singh, P., Srinivas, A., Thacker, H., Thanvi, S., Thomas, J., Adawi, F., Bashkin, A., Cohen, J., Harman-Boehm, I., Hasin, Y., Hayek, T., Iakobishvili, Z., Katz, A., Kracoff, O., Minuchin, O., Moriel, M., Mosseri, M., Omary, M., Wainstein, J., Weiss, A., Zeltser, D., Calabro, P., Derosa, G., Genovese, S., Novo, S., Olivieri, C., Piatti, P., Violini, R., Volpe, M., Ajioka, M., Amano, T., Arasaki, O., Daida, H., Fujimoto, K., Fujinaga, H., Higashiue, S., Hirohata, A., Hosokawa, S., Ikefuji, H., Inagaki, M., Iseki, H., Iwabuchi, M., Iwasaki, T., Kakishita, M., Katsuda, Y., Kawada, K., Kawajiri, K., Kawamitsu, K., Kobayashi, K., Komada, F., Komura, Y., Machida, M., Maemura, K., Matsubara, T., Matsubayashi, S., Matsumoto, T., Matsumoto, N., Mima, T., Miyamoto, N., Momiyama, Y., Morimoto, T., Murakami, M., Nakashima, E., Niijima, Y., Noda, T., Node, K., Nozaki, A., Nunohiro, T., Ogawa, T., Ono, Y., Saeki, T., Sakota, S., Sakuragi, S., Sasaki, T., Sato, Y., Sueyoshi, A., Suzuki, M., Takagi, G., Tanabe, J., Tanaka, S., Tei, I., Yamamoto, M., Yanagihara, K., Hong, T. J., Jeon, H. K., Kang, D. H., Kim, C. H., Kim, D. S., Kim, H. S., Kim, J. H., Kim, S. K., Kim, W. S., Kim, Y. K., Lee, S. R., Lee, K. W., Park, H. S., Pyun, W. B., Rha, S. W., Yoon, J., Yoon, K. H., Bennakhi, A., Geldnere, K., Sokolova, J., Teterovska, D., Dautaraite, V., Kakariekiene, V., Kavaliauskiene, R., Kucinskiene, A., Lasiene, J., Mickuviene, N., Palinauskas, A., Urboniene, A., Zilaitiene, B., Abdul Manap, H., Abidin, I. Z., Isa, S. H., Khir, A. M., Ng, K. H., Tan, F., Yusof, Z., Yusoff, K., Zambahari, R., Aguila-Marin, J., Aguilera Real, M., Alvarado-Ruiz, R., Alvarez Lopez, H., Arenas Leon, J., Bayram Llamas, E. A., Calvo Vargas, C., Carrillo Calvillo, J., Los Rios Ibarra, M., Dominguez-Reyes, C. A., Duarte, M., Elizondo, E., Fajardo Campos, P., Fanghanel-Salmon, G., Figueroa Sauceda, S., Gallegos Martinez, J., Garcia-Cantu, E., Garza Ruiz, J. A., Gonzalez Gonzalez, J. G., Guerrero Garza, M., Hernandez Herrera, C., Hernandez Munuzuri, J., Hernandez-Garcia, H., Jimenez Ramos, S., Laviada Molina, H., Lopez Villezca, D., Montano-Gonzalez, E., Nevarez Ruiz, L., Ramos Lopez, G., Reyes Araiza, R., Salazar-Gaytan, A., Salcido Vazquez, E., Sanchez Mijangos, H., Solis Morales, L., Benatar, J., Dixon, P., Nirmalaraj, K., Rosen, I., Scott, R., Young, S., Araoz Tarco, O., Barreda Cáceres, L., Benites Lopez, C., Camacho Cosavalente, L., Chavez Huapalla, E., Chois Malaga, A., Copaja Flores, A., Farfan Aspilcueta, J., Gallardo Rojas, W., Gallegos Cazorla, A., Galvez Caballero, D., Garcia Matheus, J., Garrido Carrasco, E., Gomez Sanchez, J., Hernandez Zuniga, J., Lu Galarreta, L., Luna, A., Manrique Hurtado, H., Orihuela Pastor, B., Pando Alvarez, R. M., Sanchez Povis, J., Torres Eguiluz, P., Valdivia Portugal, A., Vargas Gonzales, R., Zapata Rincon, L., Aquitania, G., Fortinez, J. T., Go, A., Gomez, M. H., Habaluyas, R., Jasul, G., Magno, M., Manalo, C. J., Mirasol, R., Morales-Palomares, E., Salvador, D. R., Sy, R. A., Tirador, L., Yao, C., Arciszewska, M., Bartkowiak, R., Czajkowska-Kaczmarek, E., Gil, R., Gniot, J., Janik, K., Janion, M., Jaworska, K., Jozwa, R., Kawecka-Jaszcz, K., Kawka-Urbanek, T., Kondys, M., Korecki, J., Korzeniak, R., Kowalisko, A., Krzeminska-Pakula, M., Kwiecien, J., Nessler, J., Odrowaz-Pieniazek, P., Piepiorka, M., Rajzer, M., Skokowska, E., Spyra, J., Sroka, M., Stasinska, T., Szymczyk, I., Trznadel-Morawska, I., Wysokinski, A., Mateus, P., Matos, P., Mimoso, J., Monteiro, P., Caballero, B., Garcia-Rinaldi, R., Gonzalez, E., Ortiz-Carrasquillo, R., Roman, A., Sierra, Y., Unger, N., Vazquez-Tanus, J., Alexandru, T., Busegeanu, M., Cozman, D. C., Fica, S., Minescu, B., Morosanu, M., Negrisanu, G. D., Pintilei, E., Pop, L., Szilagyi, I., Teodorescu, I., Tomescu, M., Barbarash, O., Chumakova, G., Churina, S., Dogadin, S., Dvoryashina, I., Esip, V., Glezer, M., Gordeev, I., Gordienko, A., Gratsiansky, N., Grineva, E., Khasanov, N., Kostenko, V., Meleshkevich, T., Mikhin, V., Morugova, T., Motylev, I., Nikolaev, K., Ponomareva, A., Repin, M., Reshetko, O., Shustov, S., Shutemova, E., Shvarts, Y., Simanenkov, V., Sobolev, K., Sukmanova, I., Timofeev, A., Tsyba, L., Varvarina, G., Vertkin, A., Vishnevsky, A., Volkov, D., Vorobiev, S., Vorokhobina, N., Yakhontov, D., Zonova, E., Zrazhevskiy, K., Damjanovic, S., Djordjevic, D., Pavlovic, M., Perunicic, J., Ristic, A., Stojkovic, S., Tasic, N., Bolvanska, N., Buganova, I., Dulkova, K., Dzupina, A., Fulop, P., Gergel, V., Kokles, M., Micko, K., Svoren, P., Urban, M., Vadinova, S., Vargova, A., Burgess, L., Coetzee, K., Du Toit, J., Gani, M., Joshi, P., Naiker, P., Nortje, H., Sarvan, M., Seeber, M., Siebert, M., Zyl, L., Wellmann, H., Calvo, C., La Hera, J., Teresa, L., Melero-Pita, A., Mesa, J., Parra Barona, J., Serrano, P., Soto, A., Tofe, S., Hornestam, B., Kempe, A., Rosenqvist, U., Rydberg, E., Tengmark, B. O., Torstensson, I., Chang, C. T., Hsia, T. L., Hsieh, I. C., Lai, W. T., Wu, C. J., Hutayanon, P., Kosachunhanun, N., Marapracertsak, M., Piamsomboon, C., Seekaew, S., Srimahachota, S., Sukhum, P., Suraamornkul, S., Tantiwong, P., Wongvipaporn, C., Amosova, K., Barna, O., Bazylevych, A., Berenfus, V., Dyadyk, A., Fushtey, I., Gyrina, O., Iabluchanskyi, M., Karpenko, O., Kaydashev, I., Korzh, O., Kulynych, R., Legkonogov, O., Mankovsky, B., Mostovoy, Y., Parkhomenko, O., Popik, G., Rudenko, L., Rudyk, I., Shevchuk, S., Sirenko, Y., Suprun, Y., Tryshchuk, N., Tseluyko, V., Vakaliuk, I., Al Mahmeed, W., Acheatel, R., Ahmad, A., Akbar, S., Akhter, F., Albirini, A., Alexander, A., Al-Joundi, B., Al-Joundi, T., Allen, G., Aloi, J., Alvarado, O., Alzohaili, O., Anderson, C., Arastu, A., Arena, C., Argoud, G., Ariani, M., Arora, C., Awasty, V., Barker, B., Barnum, O., Bartkowiak, A. J., Barzilay, J., Behrens, P., Belledonne, M., Bergman, B., Bilnoski, W., Bisognano, J., Bissette, S., Blumberg, E., Bonabi, N., Bradley, A., Breton, C., Britos, M., Broadstone, V., Budoff, M., Burge, M., Butman, S., Carroll, M., Challappa, K., Chepuri, V., Cherlin, R., Cheung, D., Coats, P., Collins, J., Cruz, H., Daboul, N., Damberg, G., David, W., Dean, J., Dedeke, E., Deeb, W., Dehaven, J., Dobs, A., Donelan, T., Dy, J., Dykstra, G., Eisen, H., Farris, N., Fattal, P., Fishman, N., Foster, M., Fredrickson, S., Gabra, N., Gabriel, J., Gatien, L., Giddings, S., Ginsberg, B., Gips, S., Glandt, M., Goldfein, A., Gordon, M., Gould, R., Graf, R., Graham, B., Graves, M., Grena, P., Hahn, R., Hamilton, D., Hamroff, G., Hanke, F., Haque, I., Harper, J., Harris, A., Harris, S., Henson, B., Hermanns, D., Herndon, W., Hershberger, V., Hyman, D., Isserman, S., Iteld, B., Jacob, M., Jaffrani, N., Jamal, A., Johnson, D., Johnson, G., Kaluski, E., Keller, R., Kereiakes, D., Khan, M., Khan, S., Klein, M., Knutson, T., Korban, E., Kozinn, M., Kraft, P., Kroeze, J., Kukuy, E., Lader, E., Laliotis, A., Lambert, C., Landau, C., Latif, K., Lee, K., Lester, F., Levenson, D., Levinson, D., Lewis, D., Litt, M., Littlefield, R., Lo, E., Lovell, C., Mahal, S., Makam, S., Mandviwala, M., Marar, I., Masri, B., Mattson, S., Mays, M., Mcgrew, F., Meengs, M., Mikell, F., Miller, M., Miranda, F., Moll, D., Multani, P., Munuswamy, K., Nallasivan, M., Nayles, L., Ong, S., Pacheco, T., Paez, H., Patel, S., Phillips, R., Pierpont, B., Prasad, J., Quinlan, E., Quion, J., Qureshi, M., Rahman, A., Raikhel, M., Ramanathan, K., Randhawa, P., Ravi, R., Reddy, R., Rendell, M., Rickner, K., Rictor, K., Rivas, J., Rosenblit, P., Rosenstock, J., Ross, S., Salacata, A., Saririan, M., Schima, S., Schlau, A., Schmedtje, J., Scott, C., Scott, D., Serru-Paez, A., Shah, R., Shah, A., Shaoulian, E., Shomali, M., Shubrook, J., Silver, K., Singh, S., Speer, J., Stevens, J., Stringam, S., Taussig, A., Taylor, A., Tee, H., Teixeira, G., Tilley, A., Toggart, E., Twahirwa, M., Unks, D., Vakili, B., Vora, K., Wang, X., Warner, A., Wefald, F., Weinberg, B., Weinstein, D., White, L., Wu, P., Yasuda, T., Yazdani, S., Yetman, C., Zarich, S., Zebrack, J., Fonseca, V. A., Mccullough, P. A., Desouza, C., Goff, D. C., Harrell, F. E., Menon, V., Sila, C., Kalahasti, V., Ahmed, S., Al Solaiman, F., Bennett, M., Cavender, M., Heil, B., Katzan, I., Monteleone, P., O Brien, B., Oommen, S., Senn, T., Sharma, J., Stegman, B., Uchino, K., Zishiri, E., Pasca, N., Brown, K., Scebbi, T., Atanasovski, I., Mccue, M., Streit, J., Oh, R., Bueno, O., Lee, D., Camisasca, R., Miyata, Y., Rubin, A., Williamson, N., Vara, S., Keeter, K., Ross, B., Los Reyes, A., Donnelly, J., Koshy-Hunt, S., Beers, B., Black, S., Buckley, M., Ephrem, M., Riley, B., West, N., Harre, M., Hsieh, R., Oshinyemi, K., Oka, Y., Matsui, N., Hoang, M., Doyle, C., Koziol, M., Lam, H., Edmonds, A., Azooz, W., Cao, C., Kim, D., Boeshaar, A., Dewindt, A., Nicholson, K., Smith, N., Hisada, M., Harding, S., Yoshioka, N., Gujral-Sandhu, K., Gans, J., Gresk, C., Kujawski, M. R., Villinski, A., Cosner, S., Johannsen, C., Barchha, N., and Knapp, B.
17. Fish Oil Enriched Intravenous Lipid Emulsions Reduce Triglyceride Levels in Non-Critically Ill Patients with TPN and Type 2 Diabetes. A Post-Hoc Analysis of the INSUPAR Study.
- Author
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Abuín-Fernández J, Tapia-Guerrero MJ, López-Urdiales R, Herranz-Antolín S, García-Almeida JM, García-Malpartida K, Ferrer-Gómez M, Cancer-Minchot E, Luengo-Pérez LM, Álvarez-Hernández J, Valera CA, Ocón-Bretón J, García-Manzanares Á, Bretón-Lesmes I, Serrano-Aguayo P, Pérez-Ferre N, López-Gómez JJ, Olivares-Alcolea J, Arraiza-Irigoyen C, Tejera-Pérez C, Martínez-González JD, Urioste-Fondo A, Abad-González ÁL, Molina-Puerta MJ, Zugasti-Murillo A, Parra-Barona J, López-Cobo I, and Olveira-Fuster AG
- Subjects
- Aged, Aged, 80 and over, Blood Glucose, Fatty Acids, Omega-3 therapeutic use, Female, Humans, Hypoglycemic Agents, Insulin, Liver metabolism, Male, Middle Aged, Parenteral Nutrition, Triglycerides blood, Diabetes Mellitus, Type 2 drug therapy, Fat Emulsions, Intravenous therapeutic use, Fish Oils therapeutic use, Parenteral Nutrition, Total adverse effects, Triglycerides metabolism
- Abstract
There are no studies that have specifically assessed the role of intravenous lipid emulsions (ILE) enriched with fish oil in people with diabetes receiving total parenteral nutrition (TPN). The objective of this study was to assess the metabolic control (glycemic and lipid) and in-hospital complications that occurred in non-critically ill inpatients with TPN and type 2 diabetes with regard to the use of fish oil emulsions compared with other ILEs. We performed a post-hoc analysis of the Insulin in Parenteral Nutrition (INSUPAR) trial that included patients who started with TPN for any cause and that would predictably continue with TPN for at least five days. The study included 161 patients who started with TPN for any cause. There were 80 patients (49.7%) on fish oil enriched ILEs and 81 patients (50.3%) on other ILEs. We found significant decreases in triglyceride levels in the fish oil group compared to the other patients. We did not find any differences in glucose metabolic control: mean capillary glucose, glycemic variability, and insulin dose, except in the number of mild hypoglycemic events that was significantly higher in the fish oil group. We did not observe any differences in other metabolic, liver or infectious complications, in-hospital length of stay or mortality., Competing Interests: The authors have no multiplicity of interest to disclose.
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- 2020
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18. Prevalence of postprandial hyperglycaemia in basal insulin-treated patients with type 2 diabetes mellitus with controlled fasting glycaemia and elevated glycosylated haemoglobin.
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Tinahones FJ, Molina-Vega M, Parra-Barona J, Flores-Le Roux J, and Gómez-Huelgas R
- Abstract
Objective: To study the prevalence of postprandial hyperglycaemia (PPH) in type 2 diabetes mellitus (T2DM) patients treated with basal insulin, having fasting glucose < 130 mg/dL but HbA1c > 7.0% (53 mmol/mol)., Methods: This was an observational prospective multicentric study conducted in Spain. During 2 weeks, patients recorded a 6-point self-measured blood glucose profile (before and 2 h after eating) every 2 days. PPH was defined according to IDF and ADA guidelines (> 160 and > 180 mg/dL, respectively)., Results: We included 98 patients (males: 56.1%; mean age: 64.3 ± 10.4 years) who were treated with basal insulin for at least 1 year at stable doses in the last 2 months, 88.8% of them received concomitant oral antidiabetic drugs. Overall, 95.7% (95% CI 91.6-99.8) and 93.5% (95% CI 88.6-98.5) of patients showed ≥ 1 episode of PPH according to IDF and ADA criteria respectively. PPH was more frequently observed after lunch and dinner. The proportion of patients with ≥ 40% readings in range of PPH was 59.1% (95% CI 49.1-69.1) and 40.9% (95% CI 30.9-50.9), according to IDF and ADA criteria, respectively., Conclusions: PPH is very common and should be considered a priority target in basal insulin-treated T2DM patients with elevated HbA1c despite controlled fasting glucose., Competing Interests: Competing interestsThe authors declared that they have no competing interests.
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- 2019
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19. Waist-to-height ratio may be an alternative tool to the body mass index for identifying Colombian adolescents with cardiometabolic risk factors.
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Aristizabal JC, Estrada-Restrepo A, and Barona J
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- Adolescent, Blood Glucose analysis, Child, Cholesterol, HDL blood, Colombia epidemiology, Cross-Sectional Studies, Female, Humans, Insulin blood, Insulin Resistance, Lipids blood, Male, Obesity blood, Obesity diagnosis, Obesity epidemiology, Overweight blood, Overweight diagnosis, Overweight epidemiology, Risk Factors, Triglycerides blood, Waist Circumference, Body Mass Index, Cardiovascular Diseases diagnosis, Cardiovascular Diseases epidemiology, Metabolic Diseases diagnosis, Metabolic Diseases epidemiology, Waist-Height Ratio
- Abstract
Introduction: Background: there is limited information about the usefulness of the waist-to-height ratio (WHtR) to identify Colombian adolescents with cardiometabolic risk factors (CRF). Objective: to compare the utility of WHtR, body mass index (BMI), and waist circumference (WC) to identify adolescents with CRF. Methods: a study with 346 youths (aged 14.0 ± 2.3 years) was performed. Anthropometric measurements were collected and BMI, WC and WHtR were calculated. Fasting blood lipids, glucose and insulin were measured; the homeostasis model assessment of insulin resistance (HOMA-IR) was computed. The presence of multiple non-WC metabolic syndrome (MetS) factors (high HOMA-IR, high triglycerides and low high-density lipoprotein cholesterol [HDL-C]) was analyzed. The area under the curve (AUC) and the odds ratios (OR) were calculated. Results: the BMI, WC and WHtR were comparable at identifying adolescents with high HOMA-IR (AUC = 0.686, 0.694 and 0.641, respectively), low HDL-C (AUC = 0.623, 0.652 and 0.572, respectively) and multiple non-WC MetS factors (AUC = 0.694, 0.715 and 0.688, respectively). The OR of having multiple non-WC MetS factors was similar in overweight adolescents (1.65, 95% CI: 0.86-3.14) and those with WHtR ≥ 0.50 (3.76, 95% CI: 1.95-7.3). There were no OR differences of having multiple non-WC MetS factors among adolescent with obesity (9.88, 95% CI: 3.1-31.7), WC ≥ P90 (18.3, 95% CI: 4.0-83.5) and WHtR ≥ 0.55 (11.0, 95% CI: 3.0-4.4). Conclusions: WHtR, BMI and WC have similar capacities to identify Colombian adolescents with CRF. WHtR showed to be an alternative tool to BMI and WC measurements when screening adolescents for cardiometabolic risk.
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- 2019
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20. Fatty Acid Content of Plasma Triglycerides May Contribute to the Heterogeneity in the Relationship Between Abdominal Obesity and the Metabolic Syndrome.
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Aristizabal JC, Barona J, Gonzalez-Zapata LI, Deossa GC, and Estrada A
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- Adult, Anthropometry, Blood Glucose analysis, C-Reactive Protein analysis, Cross-Sectional Studies, Female, Humans, Insulin blood, Insulin Resistance, Male, Metabolic Syndrome complications, Middle Aged, Obesity, Abdominal complications, Young Adult, Fatty Acids chemistry, Metabolic Syndrome blood, Obesity, Abdominal blood, Triglycerides blood
- Abstract
Background: About one-third of the people with abdominal obesity do not exhibit the metabolic syndrome (MetS). Fatty acids in plasma triglycerides (TGs) may help to explain part of this heterogeneity. This study compared TG fatty acid profile of adults with and without abdominal obesity and examined the associations of these fatty acids with MetS components., Methods: Fifty-four abdominally obese subjects were matched by age and sex with 54 adults without abdominal obesity. People were classified with MetS according to the harmonizing criteria for MetS. Fatty acids in plasma TGs were analyzed by gas chromatography., Results: There were no differences in fatty acids of plasma TGs between people with and without abdominal obesity. However, there were differences between abdominally obese people with and without MetS. The abdominally obese group with MetS had higher palmitic (+2.9%; P = 0.012) and oleic (+4.0%; P = 0.001) acids and lower linoleic (-6.4%; P = 0.018) and arachidonic (-1.2%; P = 0.004) acids. After adjustment for abdominal obesity, age, and sex, a stepwise regression analysis showed that palmitic acid positively contributed to the variance in insulin (β = +1.08 ± 1.01; P = 0.000) and homeostasis model assessment of insulin resistance (HOMA-IR) index (β = +1.09 ± 1.01; P = 0.000) and myristic acid positively contributed to the variance in systolic blood pressure (β = +1.09 ± 1.03; P = 0.006). In contrast, linoleic acid negatively contributed to the variance in glucose (β = -0.321 ± 0.09; P = 0.001) and high-sensitivity C-reactive protein (hsCRP; β = -1.05 ± 1.01; P = 0.000)., Conclusions: There were no differences in the plasma TG fatty acid profile between people with and without abdominal obesity. Likewise, fatty acids in plasma TGs associated with many of the MetS variables independently of abdominal obesity. These results suggest that the plasma TG fatty acid profile may help to explain part of the heterogeneity between abdominal obesity and the MetS.
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- 2016
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21. A Larger Body Mass Index is Associated with Increased Atherogenic Dyslipidemia, Insulin Resistance, and Low-Grade Inflammation in Individuals with Metabolic Syndrome.
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Ebron K, Andersen CJ, Aguilar D, Blesso CN, Barona J, Dugan CE, Jones JL, Al-Sarraj T, and Fernandez ML
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- Adolescent, Adult, Aged, Biomarkers blood, Body Weight, C-Reactive Protein analysis, Dyslipidemias diagnosis, Female, Humans, Inflammation diagnosis, Inflammation Mediators blood, Insulin blood, Interleukin-6 blood, Male, Metabolic Syndrome diagnosis, Middle Aged, Obesity diagnosis, Severity of Illness Index, Waist Circumference, Weight Gain, Young Adult, Body Mass Index, Dyslipidemias blood, Inflammation blood, Insulin Resistance, Lipids blood, Metabolic Syndrome blood, Metabolic Syndrome physiopathology, Obesity physiopathology
- Abstract
Background: The consequences of increased body mass index (BMI) on the metabolic disorders associated with metabolic syndrome (MetS) have not been thoroughly examined., Methods: We analyzed data from 262 individuals, 97 men and 165 women (aged 18-70 years), classified with MetS to investigate whether variations in BMI could be associated with parameters of dyslipidemia, insulin resistance, or low-grade inflammation. We hypothesized that increases in BMI would positively correlate with the major dysregulations in metabolism that define MetS. For this purpose, individuals were separated into four subgroups based on their BMI: normal weight (<25 kg/m(2)), overweight (≥25 to <30 kg/m(2)), obese (≥30 to <40 kg/m(2)), and morbidly obese (≥40 kg/m(2))., Results: As expected, body weight and waist circumference increased significantly as BMI increased (P < 0.0001). Both systolic and diastolic blood pressure were lower in the normal BMI group compared with the other three BMI groups (P = 0.001). Markers of HDL metabolism were adversely impacted in elevated BMI groups, as both high-density lipoprotein cholesterol (HDL-C) and large HDL decreased as BMI increased (P = 0.01). BMI was negatively correlated with HDL-C (r = -0.193, P = 0.002), HDL size (r = (-)0.227, P = 0.002), and large HDL (r = -0.147, P = 0.037). In addition, plasma insulin was highest in subjects classified as morbidly obese (P < 0.0001). There was also a strong positive correlation between BMI and plasma insulin (r = 0.413, P < 0.0001), whereas adiponectin, a marker of insulin sensitivity, was negatively correlated with BMI (r = -0.288, P = 0.001). Finally, BMI was positively correlated with proinflammatory C-reactive protein (r = 0.312, P = 0.0001) and interleukin-6 (r = 0.238, P = 0.001)., Conclusions: The data from this study suggest that the physiological factors associated with increased BMI exacerbate the metabolic abnormalities characteristic of MetS.
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- 2015
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22. Association between anthropometric indices and cardiometabolic risk factors in pre-school children.
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Aristizabal JC, Barona J, Hoyos M, Ruiz M, and Marín C
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- Cardiovascular Diseases diagnosis, Cardiovascular Diseases epidemiology, Child, Preschool, Colombia epidemiology, Cross-Sectional Studies, Female, Humans, Incidence, Male, Metabolic Diseases diagnosis, Metabolic Diseases epidemiology, Obesity diagnosis, Retrospective Studies, Risk Factors, Anthropometry methods, Cardiovascular Diseases etiology, Metabolic Diseases etiology, Obesity complications, Risk Assessment methods
- Abstract
Background: The world health organization (WHO) and the Identification and prevention of dietary- and lifestyle-induced health effects in children and infants- study (IDEFICS), released anthropometric reference values obtained from normal body weight children. This study examined the relationship between WHO [body mass index (BMI) and triceps- and subscapular-skinfolds], and IDEFICS (waist circumference, waist to height ratio and fat mass index) anthropometric indices with cardiometabolic risk factors in pre-school children ranging from normal body weight to obesity., Methods: A cross-sectional study with 232 children (aged 4.1 ± 0.05 years) was performed. Anthropometric measurements were collected and BMI, waist circumference, waist to height ratio, triceps- and subscapular-skinfolds sum and fat mass index were calculated. Fasting glucose, fasting insulin, homeostasis model analysis insulin resistance (HOMA-IR), blood lipids and apolipoprotein (Apo) B-100 (Apo B) and Apo A-I were determined. Pearson's correlation coefficient, multiple regression analysis and the receiver-operating characteristic (ROC) curve analysis were run., Results: 51% (n = 73) of the boys and 52% (n = 47) of the girls were of normal body weight, 49% (n = 69) of the boys and 48% (n = 43) of the girls were overweight or obese. Anthropometric indices correlated (p < 0.001) with insulin: [BMI (r = 0.514), waist circumference (r = 0.524), waist to height ratio (r = 0.304), triceps- and subscapular-skinfolds sum (r = 0.514) and fat mass index (r = 0.500)], and HOMA-IR: [BMI (r = 0.509), waist circumference (r = 0.521), waist to height ratio (r = 0.296), triceps- and subscapular-skinfolds sum (r = 0.483) and fat mass index (r = 0.492)]. Similar results were obtained after adjusting by age and sex. The areas under the curve (AUC) to identify children with insulin resistance were significant (p < 0.001) and similar among anthropometric indices (AUC > 0.68 to AUC < 0.76)., Conclusions: WHO and IDEFICS anthropometric indices correlated similarly with fasting insulin and HOMA-IR. The diagnostic accuracy of the anthropometric indices as a proxy to identify children with insulin resistance was similar. These data do not support the use of waist circumference, waist to height ratio, triceps- and subscapular- skinfolds sum or fat mass index, instead of the BMI as a proxy to identify pre-school children with insulin resistance, the most frequent alteration found in children ranging from normal body weight to obesity.
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- 2015
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23. Increased dairy consumption differentially improves metabolic syndrome markers in male and female adults.
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Dugan CE, Barona J, and Fernandez ML
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- Absorptiometry, Photon, Adiposity, Adult, Anthropometry, Biomarkers blood, Blood Glucose analysis, Body Composition, Body Mass Index, Cross-Over Studies, Energy Intake, Female, Humans, Lipids blood, Male, Metabolic Syndrome therapy, Middle Aged, Parathyroid Hormone blood, Sex Factors, Waist Circumference, Dairy Products, Diet, Metabolic Syndrome blood
- Abstract
Background: Effects of dairy consumption on metabolic health and adiposity are inconsistent. Most clinical trials have investigated dairy intake, frequently during caloric restriction, in overweight or obese populations but not in a metabolic syndrome population. We investigated the effect of increased dairy intake without caloric restriction on anthropometrics, plasma lipids, and glucose in typically low-dairy consumers who met the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) metabolic syndrome criteria., Methods: Male (n=14) and female (n=23) adults (54.1 ± 9.7 years) with metabolic syndrome were randomized to consume low-fat dairy (LFD) (10 oz of 1% milk, 6 oz of nonfat yogurt, 4 oz of 2% cheese) or carbohydrate control (CNT) (1.5-oz granola bar and 12 oz of 100% juice) foods for 6 weeks in a crossover study design. Anthropometrics, metabolic syndrome parameters, insulin resistance, and parathyroid hormone were measured. Body composition was analyzed by a dual-energy X-ray absorptiometry scan for a subset of subjects (n=22)., Results: LFD modulated metabolic syndrome parameters differently according to gender. Following LFD, men had lower glucose (95.4 ± 9.1 vs. 98.9 ± 10.6 mg/dL, P=0.048), whereas women had lower body weight (BW), waist circumference, and body mass index (P<0.01) compared to CNT. Women also had lower energy intake following LFD compared to CNT. Increases in phosphorus (a dairy nutrient) were negatively correlated with decreases in BW (r=-0.537; P<0.01) and body fat in women (r=-0.593, P<0.025), whereas the decreases in energy intake had no correlation with anthropometrics., Conclusions: Three dairy servings/day promoted small but significant improvements differentially by gender in a metabolic syndrome population.
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- 2014
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24. Effects of carbohydrate restriction and dietary cholesterol provided by eggs on clinical risk factors in metabolic syndrome.
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Blesso CN, Andersen CJ, Barona J, Volk B, Volek JS, and Fernandez ML
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- Adult, Aged, Biomarkers blood, Blood Pressure drug effects, Body Composition drug effects, C-Reactive Protein metabolism, Female, Humans, Inflammation blood, Male, Metabolic Syndrome physiopathology, Middle Aged, Motor Activity, Risk Factors, Serum Amyloid A Protein metabolism, Cholesterol, Dietary pharmacology, Diet, Carbohydrate-Restricted, Eggs analysis, Metabolic Syndrome blood
- Abstract
Background: There are a limited number of clinical interventions evaluating the effects of dietary cholesterol in individuals at elevated risk for type 2 diabetes and cardiovascular disease., Objective: To investigate the effects of whole egg intake in adults with metabolic syndrome (MetS)., Methods: Men (n = 12) and women (n = 25) with MetS were instructed to follow a moderate carbohydrate-restricted diet (<30% energy) and randomly assigned to consume either three whole eggs (EGG, n = 20) or egg substitute (SUB, n = 17)/d for 12 weeks. Dietary intake, MetS parameters, and body composition were assessed at baseline and post-intervention., Results: Total carbohydrate (P < .001) intake decreased in all participants over time. The EGG group consumed more dietary cholesterol (P < .001) and choline (P < .001) than the SUB group. MetS was reduced in both groups, with improvements noted in dyslipidemia and decreases in waist circumference (P < .01), weight (P < .001), and percent body fat (P < .001). Reductions in plasma tumor necrosis factor-α (P < .001) and serum amyloid A (P < .05) were seen in the EGG group only. Notably, increases in dietary cholesterol were associated with reductions in plasma tumor necrosis factor-α (r = -0.340, P = .04). Plasma C-reactive protein, adiponectin, interleukin-6 interleukin-10, and cell adhesion molecules were unaffected by the intervention., Conclusions: These results demonstrate that on a moderate carbohydrate background diet, accompanied by weight loss, the inclusion of whole eggs improves inflammation to a greater extent than yolk-free egg substitute in those with MetS., (Copyright © 2013 National Lipid Association. Published by Elsevier Inc. All rights reserved.)
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- 2013
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25. Egg consumption modulates HDL lipid composition and increases the cholesterol-accepting capacity of serum in metabolic syndrome.
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Andersen CJ, Blesso CN, Lee J, Barona J, Shah D, Thomas MJ, and Fernandez ML
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- Adult, Aged, Body Weight, Cholesterol, HDL chemistry, Cholesterol, HDL metabolism, Egg Yolk chemistry, Eggs analysis, Female, Humans, Lipid Metabolism, Lipoproteins, HDL chemistry, Lipoproteins, HDL metabolism, Male, Metabolic Syndrome diet therapy, Metabolic Syndrome metabolism, Middle Aged, Phospholipids analysis, Phospholipids metabolism, Cholesterol, HDL blood, Diet, Carbohydrate-Restricted, Egg Yolk metabolism, Lipids analysis, Lipoproteins, HDL blood, Metabolic Syndrome blood
- Abstract
We recently demonstrated that daily whole egg consumption during moderate carbohydrate restriction leads to greater increases in plasma HDL-cholesterol (HDL-C) and improvements in HDL profiles in metabolic syndrome (MetS) when compared to intake of a yolk-free egg substitute. We further investigated the effects of this intervention on HDL composition and function, hypothesizing that the phospholipid species present in egg yolk modulate HDL lipid composition to increase the cholesterol-accepting capacity of subject serum. Men and women classified with MetS were randomly assigned to consume either three whole eggs (EGG, n = 20) per day or the equivalent amount of egg substitute (SUB, n = 17) throughout a 12-week moderate carbohydrate-restricted (25-30 % of energy) diet. Relative to other HDL lipids, HDL-cholesteryl ester content increased in all subjects, with greater increases in the SUB group. Further, HDL-triacylglycerol content was reduced in EGG group subjects with normal baseline plasma HDL-C, resulting in increases in HDL-CE/TAG ratios in both groups. Phospholipid analysis by mass spectrometry revealed that HDL became enriched in phosphatidylethanolamine in the EGG group, and that EGG group HDL better reflected sphingomyelin species present in the whole egg product at week 12 compared to baseline. Further, macrophage cholesterol efflux to EGG subject serum increased from baseline to week 12, whereas no changes were observed in the SUB group. Together, these findings suggest that daily egg consumption promotes favorable shifts in HDL lipid composition and function beyond increasing plasma HDL-C in MetS.
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- 2013
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26. Whole egg consumption improves lipoprotein profiles and insulin sensitivity to a greater extent than yolk-free egg substitute in individuals with metabolic syndrome.
- Author
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Blesso CN, Andersen CJ, Barona J, Volek JS, and Fernandez ML
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- Apolipoproteins blood, Apolipoproteins ultrastructure, Cholesterol Ester Transfer Proteins blood, Diet, Carbohydrate-Restricted, Female, Humans, Lipoproteins, LDL blood, Lipoproteins, LDL ultrastructure, Male, Metabolic Syndrome blood, Metabolic Syndrome diagnostic imaging, Middle Aged, Particle Size, Phosphatidylcholine-Sterol O-Acyltransferase blood, Single-Blind Method, Triglycerides blood, Ultrasonography, Eggs, Insulin Resistance physiology, Metabolic Syndrome metabolism
- Abstract
Objective: We investigated if daily egg feeding, along with carbohydrate restriction, would alter lipoprotein metabolism and influence atherogenic lipoprotein profiles and insulin resistance in men and women with metabolic syndrome (MetS)., Methods: In a randomized, single-blind, parallel design, participants consumed either 3 whole eggs/day (EGG, n=20) or the equivalent amount of yolk-free egg substitute (SUB, n=17), as part of a moderately carbohydrate-restricted diet (25%-30% energy) for 12 weeks. Plasma lipids, apolipoproteins (apos), oxidized LDL (oxLDL), cholesteryl ester transfer protein (CETP) and lecithin-cholesterol acyltransferase (LCAT) activities were assessed at baseline and week 12. Lipoprotein particle concentrations and sizes were measured by nuclear magnetic resonance spectroscopy., Results: Atherogenic dyslipidemia improved for all individuals as evidenced by reductions in plasma triglycerides, apoC-III, apoE, oxLDL, VLDL particle diameter, large VDL, total IDL, small LDL, and medium LDL particles (P<0.05). Furthermore, there were increases in HDL-cholesterol, large LDL and large HDL particles (P<0.05) for all individuals. However, there were greater increases in HDL-cholesterol and large HDL particles, and reductions in total VLDL and medium VLDL particles for those consuming EGG compared to SUB (P<0.05). Plasma insulin and insulin resistance (HOMA-IR) were reduced, while LCAT activity, and both HDL and LDL diameters increased over time in the EGG group only (P<0.05)., Conclusions: Incorporating daily whole egg intake into a moderately carbohydrate-restricted diet provides further improvements in the atherogenic lipoprotein profile and in insulin resistance in individuals with MetS., (Copyright © 2013 Elsevier Inc. All rights reserved.)
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- 2013
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27. Grape consumption increases anti-inflammatory markers and upregulates peripheral nitric oxide synthase in the absence of dyslipidemias in men with metabolic syndrome.
- Author
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Barona J, Blesso CN, Andersen CJ, Park Y, Lee J, and Fernandez ML
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- Adiponectin blood, Adult, Anti-Inflammatory Agents pharmacology, Antioxidants metabolism, Antioxidants pharmacology, Biomarkers metabolism, Fruit, Humans, Inflammation metabolism, Interleukin-10 blood, Men, Metabolic Syndrome metabolism, Middle Aged, Nitric Oxide Synthase Type II genetics, Oxidative Stress drug effects, Plant Preparations pharmacology, Plant Preparations therapeutic use, Protein Isoforms, RNA, Messenger metabolism, Reference Values, Superoxide Dismutase metabolism, Up-Regulation, Anti-Inflammatory Agents therapeutic use, Antioxidants therapeutic use, Dyslipidemias metabolism, Metabolic Syndrome diet therapy, Nitric Oxide Synthase Type II metabolism, Phytotherapy, Vitis
- Abstract
We evaluated the effects of grape consumption on inflammation and oxidation in the presence or absence of dyslipidemias in metabolic syndrome (MetS). Men with MetS (n = 24), 11 with high triglycerides and low HDL and 13 with no dyslipidemia were recruited and randomly allocated to consume daily either 46 g of lyophilized grape powder (GRAPE), equivalent to 252 g fresh grapes, or placebo with an identical macronutrient composition and caloric value as GRAPE for four weeks. After a three-week washout, participants followed the alternate treatment. We measured changes between placebo and GRAPE periods in inflammatory and oxidative stress markers both in circulation and in gene expression. Changes in plasma adiponectin (p < 0.05), interleukin (IL)-10 (p < 0.005) and in mRNA expression of the inducible isoform of nitric oxide synthase (iNOS) (p < 0.25) were increased in the GRAPE compared to the placebo period only in those individuals without dyslipidemia. Additionally, plasma IL-10 was negatively correlated with NOX2 expression, a marker of oxidative stress (r = -0.55, p < 0.01), while iNOS expression was positively correlated with the expression of superoxide dismutase 2 (r = 0.642, p < 0.01), a key anti-oxidative enzyme. Grape consumption displayed anti-oxidative and increased anti-inflammatory markers in the absence of the inflammatory milieu associated with dyslipidemias.
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- 2012
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28. Grape polyphenols reduce blood pressure and increase flow-mediated vasodilation in men with metabolic syndrome.
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Barona J, Aristizabal JC, Blesso CN, Volek JS, and Fernandez ML
- Subjects
- Adult, Aged, Biomarkers blood, Brachial Artery drug effects, Brachial Artery physiology, Cross-Over Studies, Endothelium, Vascular drug effects, Endothelium, Vascular physiology, Freeze Drying, Humans, Intercellular Adhesion Molecule-1 blood, Male, Middle Aged, Nitrates blood, Nitrites blood, Placebos, Vascular Cell Adhesion Molecule-1 blood, Vasculitis drug therapy, Vasodilation physiology, Blood Pressure drug effects, Metabolic Syndrome drug therapy, Polyphenols administration & dosage, Vasodilation drug effects, Vitis chemistry
- Abstract
We evaluated the effects of grape polyphenols in individuals classified with metabolic syndrome (MetS). Men (n = 24) aged 30-70 y were randomly assigned to consume either a freeze-dried grape polyphenol powder (GRAPE) or a placebo for 30 d in a double-blind, crossover design, separated by a 3-wk washout period. Participants were asked to maintain their usual diet and physical activity during the study and abstain from consuming polyphenol-rich foods. MetS criteria including blood pressure (BP) and markers of vascular endothelial function including brachial artery flow-mediated vasodilation (FMD), plasma total nitrite + nitrate (NOx) to estimate NO production, plasma soluble intercellular adhesion molecule-1 (sICAM-1), and soluble vascular cell adhesion molecule-1 (sVCAM-1) were measured at the end of each dietary period. Systolic BP (P < 0.0025) and plasma sICAM-1 concentrations (P < 0.025) were lower, whereas the FMD response was higher (P < 0.0001), during the GRAPE compared with the placebo period. In addition, changes in sVCAM-1 concentrations between periods were positively correlated with changes in systolic BP (r = 0.45; P < 0.05). Although NOx concentrations did not differ between periods, changes in systolic BP were negatively correlated with changes in NOx concentrations (r = -0.44; P < 0.05), indicating the vasodilating properties of NO. Other MetS variables did not differ between the GRAPE and placebo periods. These results suggest that GRAPE polyphenols may potentiate vasorelaxation and reduce BP and circulating cell adhesion molecules, resulting in improvements in vascular function.
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- 2012
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29. Dietary cholesterol affects plasma lipid levels, the intravascular processing of lipoproteins and reverse cholesterol transport without increasing the risk for heart disease.
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Barona J and Fernandez ML
- Subjects
- Biological Transport physiology, Humans, Cholesterol metabolism, Cholesterol, Dietary metabolism, Heart Diseases pathology, Lipids blood, Lipoproteins metabolism
- Abstract
The associations between dietary cholesterol and heart disease are highly controversial. While epidemiological studies and clinical interventions have shown the lack of correlation between cholesterol intake and cardiovascular disease (CVD) risk, there is still concern among health practitioners and the general population regarding dietary cholesterol. In this review, several clinical studies utilizing cholesterol challenges are analyzed in terms of changes that occur in lipoprotein metabolism resulting from excess consumption of cholesterol. Dietary cholesterol has been shown to increase both LDL and HDL in those individuals who respond to a cholesterol challenge without altering the LDL cholesterol/HDL cholesterol ratio, a key marker of CVD risk. Further, dietary cholesterol has been shown to increase only HDL with no changes in LDL with average cholesterol consumption and during weight loss interventions. Ingestion of cholesterol has also been shown to increase the size of both LDL and HDL particles with the associated implications of a less atherogenic LDL particle as well as more functional HDL in reverse cholesterol transport. Other changes observed in lipoprotein metabolism are a greater number of large LDL and decreases in small LDL subfractions. All this information put together points to specific roles of dietary cholesterol in substantially altering intravascular processing of lipoproteins as well as reverse cholesterol transport.
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- 2012
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30. A Mediterranean-style low-glycemic-load diet increases plasma carotenoids and decreases LDL oxidation in women with metabolic syndrome.
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Barona J, Jones JJ, Kopec RE, Comperatore M, Andersen C, Schwartz SJ, Lerman RH, and Fernandez ML
- Subjects
- Adult, Blood Glucose analysis, Energy Intake, Female, Fruit, Humans, Lipoproteins, HDL blood, Lipoproteins, VLDL blood, Lycopene, Middle Aged, Patient Compliance, Phytosterols administration & dosage, Soybean Proteins administration & dosage, Vegetables, Carotenoids blood, Diet, Mediterranean, Lipoproteins, LDL blood, Lutein blood, Metabolic Syndrome physiopathology, beta Carotene blood
- Abstract
Thirty-five women with metabolic syndrome and high plasma low-density lipoprotein (LDL) cholesterol (≥100 mg/dl) participated in a dietary intervention consisting of a Mediterranean-style low-glycemic-load diet for 12 weeks. Participants were randomly allocated to consume diet only (n=15) or diet plus a medical food containing soy protein and plant sterols (n=20). Plasma concentrations of carotenoids, lipoprotein subfractions and oxidized LDL (OxLDL) were measured. Independent of treatment, women had a significant increase in plasma lutein (P<.0001) and β-carotene (P<.0001), while plasma lycopene was reduced (P<.05) after 12 weeks. Low-density lipoprotein cholesterol was reduced from 138±35 to 114±33 mg/dl (P<.0001). In addition, decreases were observed in the atherogenic subfractions: large very low-density lipoprotein (P<.05), small LDL (P<.00001) and medium high-density lipoprotein (P<.05). Oxidized LDL was significantly reduced by 12% in both groups (P<.01). Changes in OxLDL were inversely correlated with plasma lutein (r=-.478, P<.0001). The data indicate that women complied with the dietary regimen by increasing fruits and vegetable intake. Decreased consumption of high-glycemic foods frequently co-consumed with lycopene-rich tomato sauce such as pasta and pizza may be responsible for the lowering of this carotenoid in plasma after 12 weeks. These results also suggest that plasma lutein concentrations may protect against oxidative stress by reducing the concentrations of OxLDL., (Copyright © 2012 Elsevier Inc. All rights reserved.)
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- 2012
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31. A Mediterranean-style, low-glycemic-load diet decreases atherogenic lipoproteins and reduces lipoprotein (a) and oxidized low-density lipoprotein in women with metabolic syndrome.
- Author
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Jones JL, Comperatore M, Barona J, Calle MC, Andersen C, McIntosh M, Najm W, Lerman RH, and Fernandez ML
- Subjects
- Acacia chemistry, Adult, Aged, Apolipoproteins blood, Cardiovascular Diseases diet therapy, Cardiovascular Diseases prevention & control, Energy Intake physiology, Female, Food, Formulated, Glycemic Index, Humans, Humulus chemistry, Middle Aged, Oxidation-Reduction, Particle Size, Phytosterols metabolism, Plant Extracts pharmacology, Proanthocyanidins pharmacology, Risk Factors, Soybean Proteins metabolism, Young Adult, Diet, Mediterranean, LDL-Receptor Related Proteins blood, Lipoprotein(a) blood, Lipoproteins blood, Metabolic Syndrome blood, Metabolic Syndrome diet therapy
- Abstract
The objective was to assess the impact of a Mediterranean-style, low-glycemic-load diet (control group, n = 41) and the same diet plus a medical food (MF) containing phytosterols, soy protein, and extracts from hops and Acacia (MF group, n = 42) on lipoprotein atherogenicity in women with metabolic syndrome. Plasma lipids, apolipoproteins (apos), lipoprotein subfractions and particle size, low-density lipoprotein (LDL) oxidation, and lipoprotein (a) were measured at baseline, week 8, and week 12 of the intervention. Three-day dietary records were collected at the same time points to assess compliance. Compared with baseline, women decreased energy intake from carbohydrate (P < .001) and fat (P < .001), whereas they increased energy intake from protein (P < .001). A significant increase in energy from monounsaturated fatty acids was also observed as well as increases in eicosapentaenoic acid and docosahexaenoic acid, whereas trans-fatty acid intake was reduced (P < .00001). The atherogenic lipoproteins, large very low-density lipoprotein (P < .0001) and small LDL (P < .0001), were reduced, whereas the ratio of large high-density lipoprotein to smaller high-density lipoprotein particles was increased (P < .0001). Apolipoprotein B was reduced for all women (P < .0001), with a greater reduction in the MF group (P < .025). Oxidized LDL (P < .05) and lipoprotein (a) (P < .001) were reduced in both groups at the end of the intervention. Consumption of a Mediterranean-style diet reduces the risk for cardiovascular disease by decreasing atherogenic lipoproteins, oxidized LDL, and apo B. Inclusion of an MF may have an additional effect in reducing apo B., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
32. Comparative study of the efficacy and safety of two polyvalent, caprylic acid fractionated [IgG and F(ab')2] antivenoms, in Bothrops asper bites in Colombia.
- Author
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Otero-Patiño R, Segura A, Herrera M, Angulo Y, León G, Gutiérrez JM, Barona J, Estrada S, Pereañez A, Quintana JC, Vargas LJ, Gómez JP, Díaz A, Suárez AM, Fernández J, Ramírez P, Fabra P, Perea M, Fernández D, Arroyo Y, Betancur D, Pupo L, Córdoba EA, Ramírez CE, Arrieta AB, Rivero A, Mosquera DC, Conrado NL, and Ortiz R
- Subjects
- Adolescent, Animals, Blood Coagulation, Blood Coagulation Disorders drug therapy, Caprylates pharmacology, Chemical Fractionation methods, Chromatography, Ion Exchange methods, Colombia, Crotalid Venoms metabolism, Double-Blind Method, Drug Evaluation, Female, Fibrinogen analysis, Hemorrhage drug therapy, Humans, Immunoglobulin Fab Fragments therapeutic use, Immunoglobulin G therapeutic use, Incidence, Male, Pepsin A metabolism, Treatment Outcome, Antivenins therapeutic use, Bothrops metabolism, Drug-Related Side Effects and Adverse Reactions metabolism, Snake Bites drug therapy
- Abstract
The efficacy and safety of two polyvalent horse-derived antivenoms in Bothrops asper envenomings were tested in a randomized, double-blind, clinical trial performed in Colombia. Both antivenoms were manufactured from the same pool of hyperimmune plasma. Antivenom A was made of F(ab')2 fragments, generated by pepsin digestion and caprylic acid precipitation, whereas antivenom B consisted of whole IgG molecules produced by caprylic acid precipitation followed by ion-exchange chromatography. Besides the different nature of the active substance, antivenom B had higher protein concentration, slightly higher turbidity and aggregate content. No significant differences were observed in the efficacy of antivenoms. Both halted local and systemic bleeding (P = 0.40) within 6-12 h of treatment in 100% of the cases, and restored blood coagulation (P = 0.87) within 6-24 h in 84.7% of patients, and within 48 h in all of them, in agreement with restoration of plasma fibrinogen concentration. Venom concentrations in serum dropped significantly (P < 0.001), to very low levels, 1 h after antivenom infusion. Nevertheless, eight patients (11.1%), four for each antivenom, presented recurrence of venom antigenaemia at different times, from 6 to 96 h, with clinical significance (recurrent coagulopathy) only in one group B patient (2.9%). Serum creatine kinase (CK) activity was increased, as a consequence of local myonecrosis. There was no significant difference (P = 0.51) in the incidence of early adverse reactions to antivenom administration (28.9% for patients of group A and 20.6% for patients of group B), most of the reactions being mild, mainly cutaneous. The most frequent complications were cellulitis (16.7%), abscess formation (5.6%), acute renal failure (8.3%), and compartmental syndrome (5.6%). In conclusion, IgG and F(ab')2 antivenoms, prepared by caprylic acid fractionation, presented similar efficacy and safety profiles for the treatment of B. asper envenomings in Colombia., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
33. A Mediterranean-style low-glycemic-load diet improves variables of metabolic syndrome in women, and addition of a phytochemical-rich medical food enhances benefits on lipoprotein metabolism.
- Author
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Jones JL, Fernandez ML, McIntosh MS, Najm W, Calle MC, Kalynych C, Vukich C, Barona J, Ackermann D, Kim JE, Kumar V, Lott M, Volek JS, and Lerman RH
- Subjects
- Adult, Aged, Apolipoproteins blood, Blood Glucose analysis, C-Reactive Protein analysis, Docosahexaenoic Acids therapeutic use, Eating, Eicosapentaenoic Acid therapeutic use, Female, Glycemic Index, Homocysteine blood, Humans, Middle Aged, Waist Circumference, Young Adult, Cholesterol, LDL blood, Diet, Mediterranean, Hypercholesterolemia diet therapy, Metabolic Syndrome diet therapy
- Abstract
Background: The high prevalence of metabolic syndrome (MetS) has highlighted the need for effective dietary interventions to combat this growing problem., Objective: To assess the impact of a Mediterranean-style low-glycemic-load diet (control arm, n = 44) or the same diet plus a medical food containing phytosterols, soy protein, and extracts from hops and acacia (intervention arm, n = 45) on cardiometabolic risk variables in women with MetS., Methods: In this 12-week, 2-arm randomized trial, baseline, week 8 and 12, fasting blood samples were drawn to measure plasma lipids, apolipoproteins, and homocysteine. Dietary records were also collected and analyzed., Results: There were decreases in fat and sugar intake (P < .001 for both) and increases in docosahexaenoic acid and eicosapentaenoic acid intake (P < .001 for both) over time, consistent with the prescribed diet. Regarding MetS variables, there were decreases in waist circumference, systolic and diastolic blood pressure, and plasma triglycerides in all subjects (P < .001 for all) with no differences between arms. Plasma low-density lipoprotein cholesterol, non-high-density lipoprotein cholesterol, apolipoprotein (apo) B, and apo B/apo A1 were reduced over time but to a greater extent in the intervention arm (P < .05 for all), indicating the medical food had a greater effect in altering lipoprotein metabolism. Further, medical food intake was associated with reduced plasma homocysteine (P < .01) compared to the control arm., Conclusion: A Mediterranean-style low-glycemic-load diet effectively reduces the variables of MetS. Addition of the medical food results in a less atherogenic lipoprotein profile and lower plasma homocysteine., (Copyright © 2011 National Lipid Association. Published by Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
34. Waist circumference is positively correlated with markers of inflammation and negatively with adiponectin in women with metabolic syndrome.
- Author
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Ackermann D, Jones J, Barona J, Calle MC, Kim JE, LaPia B, Volek JS, McIntosh M, Kalynych C, Najm W, Lerman RH, and Fernandez ML
- Subjects
- Adult, Aged, Biomarkers blood, Blood Glucose analysis, Body Mass Index, C-Reactive Protein metabolism, Cross-Sectional Studies, Diet, Dietary Carbohydrates, Female, Glycemic Index, Humans, Insulin blood, Insulin Resistance, Interleukin-6 blood, Middle Aged, Adiponectin blood, Inflammation blood, Metabolic Syndrome blood, Waist Circumference
- Abstract
The purpose of this study was to correlate biomarkers of metabolic syndrome (MetS), with markers of inflammation and macronutrient intake in 89 women (25-72 years) with MetS. We hypothesized that waist circumference (WC) would have the stronger correlations with inflammatory parameters and would correlate with carbohydrate intake. Values for WC (108.7 ± 11.1 cm) and plasma triglycerides (202.7 ± 52.1 mg/dL) were elevated, whereas plasma glucose levels varied from 66 to 179 mg/dL, with 42% of women having insulin resistance. Plasma levels of interleukin 6 (0.2-15.9 mg/L), tumor necrosis factor α (1.47-12.3 mg/L), and high-sensitivity C-reactive protein (0.06-3.08 mg/dL) varied widely, with most women being above values considered normal. Subjects had high intake of total sugar (92.3 ± 56.4 g/d), high glycemic index (59.8 ± 6.5), and glycemic load (127.2 ± 56.1), whereas dietary fiber (17.1 ± 9.1 g/d) was below recommended intake. Waist circumference was positively correlated with insulin (r = 0.275, P < .01) and with the inflammatory markers interleukin 6 (r = 0.307, P < .01) and tumor necrosis factor α (r = 0.228, P < .05) and negatively correlated with plasma adiponectin (r = -0.309, P < .0001). In addition, WC was positively correlated with total carbohydrate, added sugar, and glycemic load (P < .05) but not with fat or protein. These results are consistent with central obesity being a key marker of the inflammatory state, and they also suggest that carbohydrates, particularly those that are digested rapidly, contribute to increased risk of central obesity and development of MetS., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
35. Low HDL cholesterol is associated with increased atherogenic lipoproteins and insulin resistance in women classified with metabolic syndrome.
- Author
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Fernandez ML, Jones JJ, Ackerman D, Barona J, Calle M, Comperatore MV, Kim JE, Andersen C, Leite JO, Volek JS, McIntosh M, Kalynych C, Najm W, and Lerman RH
- Abstract
Both metabolic syndrome (MetS) and elevated LDL cholesterol (LDL-C) increase the risk for cardiovascular disease (CVD). We hypothesized that low HDL cholesterol (HDL-C) would further increase CVD risk in women having both conditions. To assess this, we recruited 89 women with MetS (25-72 y) and LDL-C ≥ 2.6 mmol/L. To determine whether plasma HDL-C concentrations were associated with dietary components, circulating atherogenic particles, and other risk factors for CVD, we divided the subjects into two groups: high HDL-C (H-HDL) (≥ 1.3 mmol/L, n = 32) and low HDL-C (L-HDL) (< 1.3 mmol/L, n = 57). Plasma lipids, insulin, adiponectin, apolipoproteins, oxidized LDL, Lipoprotein(a), and lipoprotein size and subfractions were measured, and 3-d dietary records were used to assess macronutrient intake. Women with L-HDL had higher sugar intake and glycemic load (P < 0.05), higher plasma insulin (P < 0.01), lower adiponectin (P < 0.05), and higher numbers of atherogenic lipoproteins such as large VLDL (P < 0.01) and small LDL (P < 0.001) than the H-HDL group. Women with L-HDL also had larger VLDL and both smaller LDL and HDL particle diameters (P < 0.001). HDL-C was positively correlated with LDL size (r = 0.691, P < 0.0001) and HDL size (r = 0.606, P < 0.001), and inversely correlated with VLDL size (r = -0.327, P < 0.01). We concluded that L-HDL could be used as a marker for increased numbers of circulating atherogenic lipoproteins as well as increased insulin resistance in women who are already at risk for CVD.
- Published
- 2010
- Full Text
- View/download PDF
36. [Tityus asthenes scorpion stings: epidemiological, clinical and toxicological aspects].
- Author
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Gómez JP, Quintana JC, Arbeláez P, Fernández J, Silva JF, Barona J, Gutiérrez JC, Díaz A, and Otero R
- Subjects
- Adolescent, Adult, Aged, Animals, Child, Child, Preschool, Female, Humans, Infant, Male, Mice, Middle Aged, Prospective Studies, Retrospective Studies, Scorpion Venoms poisoning, Scorpions, Young Adult, Scorpion Stings diagnosis, Scorpion Stings epidemiology
- Abstract
Introduction: Scorpion stings are a public health problem in many countries. However, in Colombia, very few epidemiological, clinical or toxicological studies have been undertaken., Objective: Ecological and epidemiological aspects were related to the prevalence of scorpion stings by Tityus asthenes. The clinical features of envenomization were described in patients and in an experimental animal model., Materials and Methods: The study was conducted in four localities of Mutatá and Urabá Counties in the province of Antioquia, Colombia. The sample consisted of 1,593 (929 urban, 664 rural) of the 5,305 exposed people, inhabitating 324 households (188 urban (58%); 136 rural (42%) of 1,448 houses total in the study area. An interview survey was performed in every selected family for a more realistic estimate of sting prevalence. Additionally, a prospective study was directed toward patients presenting scorpion stings at care at the local hospital over an 18-month period., Results: The probability was 12.9 times greater of finding T. asthenes inside or around houses in places near to forest and high agrarian plantations (odds ratio = 13). Eighty scorpion stings were reported in the retrospective study (4.1% prevalence [95% CI 3.3-4.8%] ), but only 14 of the patients (17.5%) sought care in the local hospital (an 82.5% underreportage). Seventy percent of the stings occurred in rural places; 50% occurred in the locality of Caucheras, with an attack rate of 10.6%. The overall household infestation rate was 269% (95% CI 22.9-30.8%) and an area dispersion ratio of 100%. Signs of systemic envenomization occurred mainly in children (67%). The 50% lethal dose of T. asthenes venom was 121.6 µg for 18-20 g Swiss Webster rats (95% CI 103.7-139.6). Immunodetection of T. asthenes and Centruroides gracilis/C.margantatus venoms in the experimental animals was possible when were tested by Western blot against Alacramyn (Instituto Bioclón, México) and Soro antiaracnídico (Instituto Butantan, Brasil) antivenoms. Scorpion interspecific differences were noted., Conclusions: The prevalence of stings by T. asthenes were common and their presence was associated with tropical rainforests. Envenomization at low density can be neutralized efficiently by anti-scorpion antivenoms produced in México and Brazil but with differing specificities for the venom of each scorpion species.
- Published
- 2010
37. Efficacy and safety of two whole IgG polyvalent antivenoms, refined by caprylic acid fractionation with or without beta-propiolactone, in the treatment of Bothrops asper bites in Colombia.
- Author
-
Otero R, León G, Gutiérrez JM, Rojas G, Toro MF, Barona J, Rodríguez V, Díaz A, Núñez V, Quintana JC, Ayala S, Mosquera D, Conrado LL, Fernández D, Arroyo Y, Paniagua CA, López M, Ospina CE, Alzate C, Fernández J, Meza JJ, Silva JF, Ramírez P, Fabra PE, Ramírez E, Córdoba E, Arrieta AB, Warrell DA, and Theakston RD
- Subjects
- Adolescent, Adult, Aged, Animals, Antivenins blood, Antivenins chemistry, Blood Coagulation Disorders drug therapy, Blood Coagulation Disorders etiology, Caprylates pharmacology, Chemical Fractionation methods, Child, Child, Preschool, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Female, Fibrinogen metabolism, Humans, Immunoglobulin G, Male, Mice, Middle Aged, Propiolactone pharmacology, Recurrence, Snake Bites blood, Treatment Outcome, Whole Blood Coagulation Time, Antivenins therapeutic use, Bothrops, Crotalid Venoms blood, Snake Bites drug therapy
- Abstract
The efficacy and safety of two whole IgG polyvalent antivenoms (A and B) were compared in a randomised, blinded clinical trial in 67 patients systemically envenomed by Bothrops asper in Colombia. Both antivenoms were fractionated by caprylic acid precipitation and had similar neutralising potencies, protein concentrations and aggregate contents. Antivenom B was additionally treated with beta-propiolactone to lower its anticomplementary activity. Analysing all treatment regimens together, there were no significant differences between the two antivenoms (A=34 patients; B=33 patients) in the time taken to reverse venom-induced bleeding and coagulopathy, to restore physiological fibrinogen concentrations and to clear serum venom antigenaemia. Blood coagulability was restored within 6-24 h in 97% of patients, all of whom had normal coagulation and plasma fibrinogen levels 48 h after the start of antivenom treatment. Two patients (3.0%) had recurrent coagulopathy and eight patients suffered recurrence of antigenaemia within 72 h of treatment. None of the dosage regimens of either antivenom used guaranteed resolution of venom-induced coagulopathy within 6 h, nor did they prevent recurrences. A further dose of antivenom at 6 h also did not guarantee resolution of coagulopathy within 12-24 h in all patients. The incidence of early adverse reactions (all mild) was similar for both antivenoms (15% and 24%; P>0.05).
- Published
- 2006
- Full Text
- View/download PDF
38. Proteomic analysis of the venom and characterization of toxins specific for Na+ - and K+ -channels from the Colombian scorpion Tityus pachyurus.
- Author
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Barona J, Batista CV, Zamudio FZ, Gomez-Lagunas F, Wanke E, Otero R, and Possani LD
- Subjects
- Amino Acid Sequence, Animals, Cell Line, Chromatography, High Pressure Liquid, Humans, In Vitro Techniques, Lethal Dose 50, Mice, Molecular Sequence Data, Potassium Channel Blockers chemistry, Potassium Channel Blockers isolation & purification, Potassium Channel Blockers toxicity, Proteomics, Scorpion Venoms genetics, Scorpions chemistry, Scorpions genetics, Scorpions pathogenicity, Sequence Homology, Amino Acid, Shaker Superfamily of Potassium Channels antagonists & inhibitors, Sodium Channel Blockers chemistry, Sodium Channel Blockers isolation & purification, Sodium Channel Blockers toxicity, Spectrometry, Mass, Electrospray Ionization, Potassium Channels drug effects, Scorpion Venoms chemistry, Scorpion Venoms toxicity, Sodium Channels drug effects
- Abstract
The Colombian scorpion Tityus pachyurus is toxic to humans and is capable of producing fatal accidents, but nothing is known about its venom components. This communication reports the separation of at least 57 fractions from the venom by high performance liquid chromatography. From these, at least 104 distinct molecular weight compounds were identified by mass spectrometry analysis. The complete amino acid sequences of three peptides were determined and the partial sequences of three others were also identified. Electrophysiological experiments conducted with ion-channels expressed heterologously on Sf9 cells showed the presence of a potent Shaker B K(+)-channel blocker. This peptide (trivial name Tpa1) contains 23 amino acid residues closely packed by three disulfide bridges with a molecular mass of 2,457 atomic mass units. It is the third member of the sub-family 13, for which the systematic name is proposed to be alpha-KTx13.3. The mice assay showed clearly the presence of toxic peptides to mammals. One of them named Tpa2, containing 65 amino acid residues with molecular mass of 7,522.5 atomic mass units, is stabilized by four disulfide bridges. It was shown to modify the Na(+)-currents of F-11 and TE671 cells in culture, similar to the beta scorpion toxins. These results demonstrate the presence of toxic peptides in the venom of T. pachyurus and confirm that accidents with this species of scorpion should be considered an important human hazard in Colombia.
- Published
- 2006
- Full Text
- View/download PDF
39. [Toxicological and immunological aspects of scorpion venom (Tytius pachyurus): neutralizing capacity of antivenoms produced in Latin America].
- Author
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Barona J, Otero R, and Núñez V
- Subjects
- Animals, Colombia, Immunochemistry, Latin America, Mice, Neutralization Tests, Antivenins immunology, Scorpion Venoms immunology, Scorpion Venoms toxicity
- Abstract
The toxicity and immunochemical properties of Tityus pachyurus Pocock scorpion venom was characterized, as well as the neutralization capacity against it by three anti-scorpion antivenoms (Alacramyn, Instituto Bioclón, México; Suero antiescorpiónico, Instituto Butantán, Sao Paulo, Brasil; and Suero antiescorpiónico, Centro de Biotecnología, Universidad Central de Venezuela, Caracas, Venezuela). The venom yield, obtained by manual milking, 680+/-20 microg venom, a 50% lethal dose in mice was 4.8 microg/kg (90 microg for an 18-20 g mouse). The most common symptoms of venom poisoning in mice were sialorrhea, respiratory distress, profuse sweating, ataxia, behavior alterations (restlessness, somnolence) and hyperglycemia at 3 and 24 hours after subcutaneous venom injection (0.5 LD50). The neutralizing capacity of Bioclón (México City) and Butantán (Sao Paulo) antivenoms (for a 50% effective dose) was 330 and 292 microg venom/ml antivenom, respectively. The Biotecnología (Caracas) antivenom did not neutralize the lethal effect of venom. By electrophoresis (SDS-PAGE) was demonstrated that the venom contains proteins from less than 14 kd to 97 kd. The Western blots indicated immunological reactivity of the three antivenoms with most of venom components, including proteins of low molecular mass (<14 kd). The results allow to conclude that T. pachyurus venom is neutralized efficiently by anti-scorpion antivenoms produced in México and Brasil.
- Published
- 2004
40. Images of the nervous system in Cajal's scientific thought.
- Author
-
Barona JL
- Subjects
- History, 19th Century, History, 20th Century, Spain, Nervous System
- Abstract
Since his death in 1934, a wide range of publications have outlined Santiago Ramon y Cajal's (1852-1934) scientific personality as well as his contribution to the acceptance of the neuron theory. Trying to go beyond the traditional biographical approach, the present paper aims to set up the historical origins and the development of his scientific conributions. His global image of the nervous system, the neuron theory and other aspects of his scientific views are also related to his intellectual background. Not only his scientific papers, but also his own remembrances, biographical texts and general reflections are taken into account to describe the historical context of Cajal's scientific work, his discovery of the dynamic polarization of neuron cells and his contributions to the knowledge of the texture of the nervous centers and cells, the regeneration and degeneration of nerves, as well as Cajal's scientific bibliogaphy.
- Published
- 1999
41. Cancer patients and medical practice. Some historical and cultural considerations.
- Author
-
Barona JL
- Subjects
- Attitude of Health Personnel, Attitude to Health, Communication, Delivery of Health Care, Guidelines as Topic, Health, Hospital-Patient Relations, Humans, Social Adjustment, Sociology, Truth Disclosure, Culture, Neoplasms physiopathology, Neoplasms psychology, Physician-Patient Relations
- Published
- 1997
- Full Text
- View/download PDF
42. [Illness and death in the city of Valencia (1901-1920)].
- Author
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Barona JL and Barea E
- Subjects
- Demography, Developed Countries, Europe, Population, Population Characteristics, Population Dynamics, Research, Spain, Cause of Death, Data Collection, Mortality, Sex Factors, Urban Population
- Published
- 1996
43. [Alistair C. Crombie (1915-1996)].
- Author
-
Barona JL
- Subjects
- History, 20th Century, Science history, United Kingdom, Historiography
- Published
- 1996
44. [The averroism in the corpuscular philosophy of Petrus of Oleza].
- Author
-
Barona JL
- Subjects
- Europe, History, 15th Century, History, 16th Century, History, Medieval, Spain, Human Body, Philosophy, Medical history
- Abstract
Oleza is an obstinate defender of Atomism in the Spanish scientific world of the XVIth century. He was the author of a Summa totius philosophiae et medicinae, published in 1536, five years after his death. Probably, Oleza's stay in Montpellier and Pisa conciliated his critical disposition towards Scholastic Galenism. His scientific thought must be connected not only with the flourishing Atomism of the first half of XVIth century, but also with Scholastic Aristotelism deriving from the Arabic transmission and from its main representative, Averroes. Oleza's natural philosophy of the human body is strongly influenced by Averroes' ideology. This article supplies new elements about his life based on investigations of archives.
- Published
- 1995
45. [Gómez Pereira (1500-ca. 1558) and the Renaissance debate about the sensibility of animals].
- Author
-
Barona JL
- Subjects
- Animal Population Groups, Animals, History, 16th Century, Sensation, Spain, Philosophy history
- Published
- 1994
46. [Hyperthyroidism as a cause of irreversible dilated myocardiopathy].
- Author
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Parra Barona J, Cabanillas A, Jiménez Huelva JL, Pérez Garrido A, Sáenz Aranzubia P, and Díaz Pérez F
- Subjects
- Adult, Humans, Male, Cardiomyopathy, Dilated etiology, Graves Disease complications
- Abstract
We describe the case of a young man with Graves Basedow's Disease as the unique cause of congestive heart failure, which after several months of antihyperthyroid therapy not very strictly followed, underwent heart transplantation due to severe ventricular and diastolic disfunction. The relationship between thyroid hypertension and cardiomyopathy is reviewed.
- Published
- 1993
47. The body republic: social order and human body in Renaissance medical thought.
- Author
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Barona JL
- Subjects
- Europe, History, 15th Century, Humans, Anatomy history, Philosophy, Medical history, Physiology history, Religion and Medicine
- Abstract
The representation of the human body built by medicine had historical references and analogical relations with other compounds of the culture of each particular period. The organic model, the coordinated and hierarchical dependence of the body parts, its subordination to a prevailing element (the brain or the heart, depending on the authors and times) guided directly by a soul infused by God... These are some of the aspects which reflect the relation between the image of the body and the justification of the ideological and social order, as a natural one. Among the numerous sources of Renaissance medicine that could bring significant facts about this theme, the present work is based on anatomical treatises and books of natural philosophy like those written by Bernardino Montaña de Monserrate, Alonso de Fuentes, Realdo Colombo, Hieronimus Montaltus, Andrea Cesalpino and Miguel Sabuco, all of whom are good exponents of Renaissance anatomy and physiological thought.
- Published
- 1993
48. [The allegorical body: Renaissance keys to an interpretation of human nature].
- Author
-
Barona JL
- Subjects
- History, Early Modern 1451-1600, History, Modern 1601-, Anatomy history, Philosophy history, Psychology history, Symbolism
- Published
- 1993
49. [Medical theories and the classification of causes of death].
- Author
-
Barona JL
- Subjects
- Delivery of Health Care, Demography, Health, Health Services, Mortality, Population, Population Dynamics, Research, Cause of Death, Classification, Medicine, Methods, Terminology as Topic
- Abstract
"This work sketches the main stages in the evolution of medical thought and their effect upon the creation of systems intended to classify illness....[It analyzes] the influence of the three stages of the creation of the laboratory medicine in the 19th century....Lastly, it makes a proposal and a plan for a retrospective terminological glossary that should include the nosological lexicon and death causes by means of the integration of the existing empirical material." (SUMMARY IN ENG AND FRE), (excerpt)
- Published
- 1993
50. [Perspectives on pancreas transplantation in diabetes mellitus type 1].
- Author
-
Hawkins Carranza F, López Alvarez MB, and Parra Barona J
- Subjects
- Humans, Diabetes Mellitus, Type 1 surgery, Pancreas Transplantation
- Abstract
Pancreatic transplantation (PT) is a therapeutical procedure which is being currently assessed for the treatment of insulin-dependent Diabetes Mellitus. Organ PT is a real alternative, whereas islets PT is a method still in its clinic and animal experimental stages. Different surgical methods of drainage, preservation, selection of donor, complications and immunosuppressive therapy are analyzed.
- Published
- 1991
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