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Conservation of bats declining from white-nose syndrome (WNS) impacts requires an understanding of both temporal and landscape-level habitat relationships. Traditionally, much of the research on bat ecology has focused on behavior of summer maternity colonies within species’ distribution cores, including that of the endangered northern long-eared bat (Myotis septentrionalis). To further our knowledge of this species, we evaluated multi-season activity patterns in eastern North Carolina and Virginia, including areas where populations were recently discovered. We used passive acoustic monitoring to assess relative and probable activity of northern long-eared bats from October 2016 to August 2021. Northern long-eared bat relative activity was greatest in areas containing greater proportions of woody wetlands and upland pine-dominated evergreen forests. However, the likelihood of recording northern long-eared bats was associated with smaller proportions of woody wetlands and open water resources. Furthermore, we observed a higher probability of recording northern long-eared bats during non-winter seasons. Probable activity was greatest at temperatures be- tween 10 and 25 C, potentially highlighting an optimal thermoneutral zone for the species regionally. Relative activity of northern long-eared bats on the Coastal Plain of Virginia and North Carolina was primarily driven by cover features, whereas probable activity was driven by a combination of cover features, seasonality, and temperature. Therefore, acoustical surveys for this species may be most effective when targeting woody wetlands adjacent to upland forests, particularly upland pine-dominated evergreen stands, during moderate temperatures of non-winter seasons (1 April–15 November). Moreover, conservation of a diverse mosaic of woody wetlands juxtaposed by upland forests may promote both roosting and overwintering habitat, thereby enhancing overwintering survival, maternity colony establishment, and ultimately

Since the spread of white-nose syndrome in North America, several bat species have shown precipitous declines in abundance and distribution. With lower netting detection probabilities for the currently threatened but proposed endangered northern long-eared bat (Myotis septentrionalis) and endangered Indiana bats (Myotis sodalis), determination of presence or absence for regulatory clearance often has shifted to the use of acoustic surveys. However, acoustic surveys are unable to differentiate between non-reproductive individuals versus a maternity colony. We used recorded nightly echolocation pass counts of bat species-specific probabilities with maximum likelihood estimator (MLE) scores to determine thresholds by cover type and reproductive period whereby the potential for northern long-eared bat or Indiana bat maternity colonies occurs. Where nightly MLE P-values were < 0.05) were higher for sites with observed maternity activity for both bat species across forest, forest-field edge, and riparian areas versus sites where no maternity activity was known. For northern long-eared bats, nightly pass counts were highest in the juvenile volancy period (after 15 July) whereas, for Indiana bats, nightly pass counts were highest in the lactation period (16 June to 15 July). Except for edge conditions for northern long-eared bats, a MLE P < 0.05 combined with nightly pass counts above thresholds developed from surveys at known maternity colony sites for both species may indicate potential presence of a maternity colony locally and provide a tool to more efficiently use targeted mist-netting for further determination.

As the federally threatened northern long-eared bat Myotis septentrionalis continues to decline due to white-nose syndrome (WNS) impacts, the application of effective conservation measures is needed but often hindered by the lack of ecological data. To date, recommended management practices have been adopted in part from other federally listed sympatric species such as the endangered Indiana bat M. sodalis. During the maternity season, these measures have largely focused on conservation of known day-roost habitat, often with little consideration for foraging habitat, particularly riparian areas. We examined acoustic activity of northern longeared bats relative to day-roost and capture data at coastal and interior sites in the District of Columbia, New York, Pennsylvania, Virginia, and West Virginia, USA, over the course of 6 summers (2015-2020), where maternity activity was still documented after the initial arrival and spread of WNS. Acoustic activity of northern long-eared bats relative to forest cover decreased at the acoustic site level (fine scale) but increased at the sampling region level (coarse scale). We observed a positive association of northern long-eared bat acoustic activity with riparian areas. Additionally, we observed higher levels of activity during pregnancy through early lactation period of the reproductive cycle prior to juvenile volancy. Our findings suggest the need for more explicit inclusion of forested riparian habitats in northern long-eared bat conservation planning. Acoustic sampling in spring and early summer rather than mid- to late summer and in forested riparian areas is the most effective strategy for identifying potential active northern long-eared bat maternity colonies on the local landscape.

In North America, Myotis septentrionalis (Northern Long-eared Bat) has experienced precipitous declines from white-nose syndrome. As these bats become rare and difficult to capture, additional day-roost assessments to inform management may fill gaps in our understanding, particularly in habitats and regions where such roosts have never been surveyed. Over 2 summers, we radio-tracked 16 individuals from a maternity colony on Long Island, NY, in a small forested patch surrounded by development and ocean. These bats disproportionately selected small, suppressed Robinia pseudoacacia (Black Locust) trees or snags for roosting. Generally, roosts occurred within the interior or edges of this forest patch, rather than surrounding suburbia, reinforcing the hypothesis that Northern Long-eared Bats are forest adapted. Our study shows even small tracts of forest in coastal, urban areas may have conservation value in providing day-roost and foraging habitat.

We assessed the sampling effort requirements for detecting the presence of extant bat species following the impact of white-nose syndrome in the mid-Atlantic region of the United States. We acoustically sampled 27,796 nights across 846 sites between 15 May and 15 August 2016-2018 within the District of Columbia, Maryland, Pennsylvania, Virginia, and West Virginia. We developed simulations to determine the number of sites required to document bat species when each site was sampled different numbers of nights. We examined these simulations with respect to land cover, physiographic region, and time period. We generally found that sampling a greater number of sample sites within a survey area increased detection more than increasing the number of nights at individual sampling sites. The sampling effort required to detect a given bat species varied by species, as well as land-cover type and physiographic region. Our results suggest that land managers and researchers should use caution in using protocols developed with other objectives, e.g., the U.S. Fish and Wildlife Service endangered and threatened bat species and the North American Bat monitoring programs? methods are designed relative to their specific needs. Unfortunately, neither protocol may be adequate for accurately detecting bat communities within all mid-Atlantic areas.