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Experimental animal and human biopsy material has been evaluated for the possibility of culturing skin epidermocytes. Insulin and hydrocortisone were used as biostimulants of adequate cell proliferation. Morphological studies revealed that the culture obtained exists as a monolayer of epidermocytes free of other skin elements.

The growth of the culture of epidermocytes of man has been studied. Regardless of the initial density of seeding the authors revealed a considerable difference in the structure of the cellular layer and the rate of the cell proliferation at separate parts of the fundus. In seedings of diluted cellular suspensions the authors observed insignificant increase in the rate of proliferation.

A split thickness skins of burned patients were treated by trypsin and received the epidermocyte suspension. In that suspensions the content of basal keratinocytes was 10 to 70% in dependence from the treating conditions. As a rule, cultivation was a success only in those cases, when among basal keratinocytes of initial suspension were cells, synthesized DNA. Cultures were grown more rapidly under decrease of ion calcium concentration in nutritional medium up to 0.14 mM and in the presence of cell-free surface of substratum. At such conditions connections between cells were weakened, desmosomes disappeared, stratification and colony formation were broken.