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Aim: Myanmar, an Indo‐Burmese biodiversity hotspot, lacks baseline data on species occurrence and distribution. This hinders biodiversity monitoring and optimisation of conservation and development plans. We aim to document baseline mammal occupancy, interactions with environmental factors and scale‐dependent responses. Location: Hkakaborazi National Park, Htamanthi Wildlife Sanctuary, Alaungdaw Kathapa National Park, Rakhine Yoma Elephant Range, Say Taung and Myinmoletkhat Key Biodiversity Areas distributed across Myanmar. Methods: Camera trap data throughout Myanmar were used to analyse species occupancy. We conducted a multiscale hierarchical spatial modelling process, using local and pooled data across Myanmar. We also optimised spatial scale across five scales and six predictors, using univariate occupancy models. We then selected scale‐optimised variables for multivariate modelling, repeating this process for each species across local, regional and national datasets. Results: The study identified 47 terrestrial species and observed strong scale‐dependent nonstationarity in occupancy estimates. Relationships with environmental variables differed among species and were highly scale dependent. Importantly, occupancy estimates produced by pooling data across sites were greatly different from any of the estimates for the individual sites, suggesting that high heterogeneity in occurrence and abundance across sites among species requires local or nested occupancy estimates to account for spatial heterogeneity and variation. Main Conclusions: Future conservation efforts should focus on Northern Myanmar if range‐restricted and rare species are to be protected, while focus should still be given to common species which serve as potential indicators of overall community structure. The nonstationarity of occupancy results from different datasets underscores the potential for misleading interpretations from aggregated data in nonstationary ecological systems. Metareplicated analyses of local, geographically and ecologically proximal regional datasets provide an important view of spatial variation in occupancy patterns guiding conservation design and improving understanding of the drivers of biodiversity patterns and change across large regions, such as Southeast Asia. [ABSTRACT FROM AUTHOR]

The natural radionuclides in ancient El-Qasr village located in the El-Dakhla oasis (EDO), Egypt's Western Desert were measured using gamma-ray spectrometry equipped with a NaI (Tl) detector. The findings indicate that the samples' mean activity concentrations were 18.61 ± 1.02, 16.67 ± 0.9, and 137.67 ± 6.9 Bq kg−1 for 226Ra, 232Th, and 40K, respectively. The values of Raeq, D, AED, Hex, Hin, Iγ, and ELCR for the samples ranged anywhere from 34.1 to 83.9 Bq kg−1, 15.7 to 37 nGy h−1, 19.26 to 45.384 Sv y−1, 0.09 to 0.23, 0.12 to 0.23, 0.25 to 0.59, and 6.74E−05 to 1.59E−04, respectively. These values are significantly lower than the international limit of 370 Bq kg−1 for Raeq, 59 nGy h−1 for D, 70 Sv y−1 for AED, 1 for Hex and Hin, 2 for Iγ, and 29E−03 for ELCR. According to the obtained data, none of the samples seemed to be a significant risk when it came to radiation exposure. Using these data, we will determine the baseline level of radionuclides that occur naturally in the area that is the subject of the inquiry. [ABSTRACT FROM AUTHOR]

Aims: Ecological restoration is informed by established guidelines, including the use of reference ecosystems to direct restoration objectives and quantify progress. Despite their importance, the use of reference conditions in restoration projects has been inconsistent, particularly where habitat degradation has occurred at landscape scales. Here we use baseline vegetation data and the relationship between vegetation communities and environmental conditions to identify and select positive reference sites within a proposed landscape‐scale restoration project. In addition, we demonstrate the use of positive references in monitoring through an adjacent post‐restoration case study area. Location: Wild Nephin National Park, Mayo, Ireland. Methods: Vegetation communities at sites (n = 230) within a large (4700 ha) proposed restoration area were surveyed using quadrats. Positive reference sites were identified following community classification within the Irish Vegetation Classification (IVC). The environmental determinants of positive references were assessed through redundancy analysis. Based on these determinants, sites with similar environmental conditions were identified though k‐medoids clustering, enabling positive references to be spatially designated. Ordination and Ellenberg's indicator values were used to demonstrate the use of positive references in restoration monitoring. Results: A total of 49 positive reference sites were identified for the study area. Altitude, soil type, subsoil type, soil depth and topographical position were identified as significant determinants of communities within positive reference sites. K‐medoids clustering with 11 clusters accurately grouped environmentally similar regions of the study area and enabled positive reference sites to be determined and designated spatially based on the cluster groupings. Conclusions: Overall, the study demonstrates with a practical example how landscape‐scale restoration can be informed and monitored through the identification of appropriate positive reference sites within the restoration area, based on the relationship between existing non‐degraded plant communities and their environmental determinants, derived from baseline habitat data. [ABSTRACT FROM AUTHOR]

Responsible stock enhancement initiatives require baseline data of wild population demographic conditions that can be used in testing management outcomes. This study provides the first fishery-independent assessment of length- and age-based biological characteristics of exploited populations of Platycephalus fuscus in eastern Australia prior to stock enhancement. Sampling was conducted over seven estuaries spanning seven degrees of latitude and the geographical range of proposed stock enhancements. Populations in all estuaries showed evidence of length and age truncation, especially those subject to commercial fisheries where young individuals of both sexes dominated populations. Maximum longevities were 12 and 11 years for females and males respectively, but few females >5 years and males >3 years were generally sampled. Females dominated populations, and on average, the mean lengths and ages of females were greater than males within each estuary and across all age classes. Sexually dimorphic variation in growth was evident across all estuaries, with females attaining greater maximum lengths than males. Estuary-specific differences in individual growth were not identified. On average, over 50% of females sampled in each estuary were > the minimum legal length (MLL), but the opposite was evident for males. In contrast, over 25% of males in each estuary were > the mean length at maturity (L50), whereas in all but one estuary the L50. Stocked male and female P. fuscus should recruit to the fishery in 2 and 3 years, and contribute to the spawning stock in 1.5 and 4.5 years, respectively. This study provides important historical baseline data that can contribute to testing stock enhancement outcomes on populations.

Changes to biodiversity from urbanization are occurring worldwide, and baseline data is vital to document the magnitude and direction of these alterations. We set out to document the biodiversity of an urban lake in Eastern Iowa that was devoid of baseline data prior to a renovation project that will convert the site into a major area for human recreation. Throughout the course of one year, we studied the biodiversity at Cedar Lake utilizing the citizen-science application iNaturalist coupled with semi-structured BioBlitz events, which we compared to previous opportunistic observations at the site. From a semi-structured approach to document biodiversity with citizen science, our analyses revealed more diverse community metrics over a shorter period compared to more than a decade of prior observations. [ABSTRACT FROM AUTHOR]

Responsible stock enhancement initiatives require baseline data of wild population demographic conditions that can be used in testing management outcomes. This study provides the first fishery-independent assessment of length- and age-based biological characteristics of exploited populations of Platycephalus fuscus in eastern Australia prior to stock enhancement. Sampling was conducted over seven estuaries spanning seven degrees of latitude and the geographical range of proposed stock enhancements. Populations in all estuaries showed evidence of length and age truncation, especially those subject to commercial fisheries where young individuals of both sexes dominated populations. Maximum longevities were 12 and 11 years for females and males respectively, but few females >5 years and males >3 years were generally sampled. Females dominated populations, and on average, the mean lengths and ages of females were greater than males within each estuary and across all age classes. Sexually dimorphic variation in growth was evident across all estuaries, with females attaining greater maximum lengths than males. Estuary-specific differences in individual growth were not identified. On average, over 50% of females sampled in each estuary were > the minimum legal length (MLL), but the opposite was evident for males. In contrast, over 25% of males in each estuary were > the mean length at maturity (L50), whereas in all but one estuary <13% of the females were > the L50. Stocked male and female P. fuscus should recruit to the fishery in 2 and 3 years, and contribute to the spawning stock in 1.5 and 4.5 years, respectively. This study provides important historical baseline data that can contribute to testing stock enhancement outcomes on populations. [ABSTRACT FROM AUTHOR]

Genetically engineered (GE) crops have the potential to contribute to agricultural sustainability, food security, and nutritional enrichment. However, these crops cannot be released for commercial cultivation without undergoing environmental risk assessments (ERA), thus biosafety evaluation. ERA assessments are performed comparatively with their natural non-GE counterparts. As Bangladesh is progressing with GE potato research, the present study aims to collect baseline information on non-GE potato cultivation with an emphasis on current agronomic practices focusing on fertilizer management and farmers’ knowledge base. The survey had three parts, including information on the farmers, information on potato cultivation practices, especially fertilizer use, and lastly, the farmer’s view on GE potato. From 2020 to 2021, data were collected through interviews with experienced growers in four potato-growing regions, the Central and Mid-East, North-West, Mid-West, and South-East regions (n = 1757) of the country. The study revealed that farmers of all regions used more than the recommended amounts of fertilizer; for instance, 67.1% more nitrogen fertilizer was applied as an extra dose during potato cultivation in Munshiganj (Central and Mid-East) than in the Dinajpur region (North-West). This overuse of nitrogen fertilizer can enhance plant vigor but makes the plants more susceptible to insect attraction and allows pests easier access to the plants. As a result, the excess dose of nitrogen fertilizer in Munshiganj may act as a catalyst to increase the probability of late blight. The findings also showed that 73.6% of farmers observed unexpected flowering in certain potato cultivars, which corresponded to the higher application of phosphate and potassium fertilizers aimed at late blight control. Furthermore, this study reported infestations of Solanaceous weeds, specifically Solanum torvum and Physalis heterophylla, in potato fields. Finally, our findings demonstrated that more than 68.7% of the potato growers intend to adopt disease-resistant GE potato as that may reduce the need for excess fertilizer use and thus reduce cultivation costs.

Changes to biodiversity from urbanization are occurring worldwide, and baseline data is vital to document the magnitude and direction of these alterations. We set out to document the biodiversity of an urban lake in Eastern Iowa that was devoid of baseline data prior to a renovation project that will convert the site into a major area for human recreation. Throughout the course of one year, we studied the biodiversity at Cedar Lake utilizing the citizen-science application iNaturalist coupled with semi-structured BioBlitz events, which we compared to previous opportunistic observations at the site. From a semi-structured approach to document biodiversity with citizen science, our analyses revealed more diverse community metrics over a shorter period compared to more than a decade of prior observations.

Background: To investigate the baseline data characteristic, human leukocyte antigen (HLA) polymorphisms, and panel reactive antibody (PRA) in end-stage kidney disease (ESKD) patients awaiting kidney transplantation in Southwest China. Methods: HLA genotyping was performed using the real-time PCR sequence-specific primer. PRA was detected by enzyme-linked immunosorbent assay. The patients' medical records were extracted from the hospital information database. Results: A total of 281 kidney transplant candidates with ESKD were analyzed. The average age was 35.7 ± 13.8 years. There were 61.6% patients had hypertension, 40.2% patients had dialysis ≥ 3 times per week, 47.3% patients had moderate or severe anemia, 30.2% patients with albumin < 35 g/L, 49.1% patients had serum ferritin < 200 ng/mL, 40.5% patients had serum calcium in target range (2.23 - 2.80 mmol/L), 43.4% patients had serum phosphate in target range (1.45 - 2.10 mmol/L), and 93.6% patients with parathyroid hormone > 88.00 pg/mL. In total, 15 HLA-A, 28 HLA-B, 15 HLA-DRB1, and 8 HLA-DQB1 allelic groups were identified. The most frequent alleles for each locus were HLA-A*02 (33.63%), HLA-B*46 (14.41%), HLA-DRB1*15 (21.89%), and HLADQB1* 05 (39.50%). The most frequent haplotypes were HLA-A*33-B*58-DRB1*17-DQB1*02. A total of 9.60% of patients tested positive for PRAs - Class I or Class II. Conclusions: The data from this study provide some new insights into baseline data, the distribution of HLA polymorphisms, and PRA results in the population of Southwest China. This is of great significance in this region, and indeed in the country as a whole, in comparison with other populations and in the process of organ transplant allocation. [ABSTRACT FROM AUTHOR]

Introduction: The mucogingival junction is considered one of the vital anatomic structures that mark the apical termination of the attached gingiva. However, due to various pathologies and conditions, it can be undetectable. This study aimed to assess the distribution of patients having teeth without clinically detectable mucogingival junction in mandibular anteriors. Various etiologies leading to clinically undetectable mucogingival junction were also identified. Methods: This cross-sectional study involved 312 patients who visited the department of Periodontics of Gandaki Medical College Teaching Hospital and Research Center, Pokhara from July 26 to November 26, 2022. The visual method, rolling probe method, tension test and histochemical staining were used to detect the mucogingival junction. To ascertain the relationship between age and gender with the distribution of data, descriptive analysis and the Pearson Chi-square test was used. A p-value

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The concentrations of Cd, Cr, Cu, Fe, Ni, Pb, & Zn were determined in the samples of soil, dismantling dust and rubber collected from the electronic waste dumping site of Shershah market and rubber from the Local market of Karachi city Pakistan. The city e-waste dumping and dismantling sites toxicity were not conducted before using modern techniques. The subsequent data of the heavy metals concentration were obtained using Atomic Absorption Spectrometry (AAS), Scanning Electron Microscopy (SEM), and Wavelength Dispersive- X-Ray Fluorescence Spectrometry (WD-XRF). Cu, Pb, and Zn were found as 133.17, 104.53 & 113.26 in soil, whereas in dismantling dust Pb & Zn were remained 10.56 and 12.65 mg/kg similarly. The China toy particle analysis by SEM was resulting metallic trend as Fe > Zn > Pb > Cd > Ni > Cr. The estimated data were compared with the levels allowed by the Pakistan Environmental Protection Agency (Pak-EPA) and the United States Environmental Protection Agency (USEPA). The maximum allowable limit for Cd is 3; Cr, Cd, and Pb are 100 for Ni is 50 and 300 mg/kg for Zn. The presence of these heavy metals from e-waste dumping would become soon a significant reason to cause serious health problems for the nearby residents and as well as city too. It has been concluded that the dumping of e-waste is the major source of contamination of heavy metals in the studied media. It is recommended that the e-waste must be recycled formally to prevent the soil from being polluted. .

Background Anaemia is common in chronic kidney disease (CKD) and assessment of the risks and benefits of new therapies is important. Methods The Anaemia Study in CKD: Erythropoiesis via a Novel prolyl hydroxylase inhibitor Daprodustat-Non-Dialysis (ASCEND-ND) trial includes adult patients with CKD Stages 3–5, not using erythropoiesis-stimulating agents (ESAs) with screening haemoglobin (Hb) 8–10 g/dL or receiving ESAs with screening Hb of 8–12 g/dL. Participants were randomized to daprodustat or darbepoetin alfa (1:1) in an open-label trial (steering committee- and sponsor-blinded), with blinded endpoint assessment. The co-primary endpoints are mean change in Hb between baseline and evaluation period (average over Weeks 28–52) and time to first adjudicated major adverse cardiovascular (CV) event. Baseline characteristics were compared with those of participants in similar anaemia trials. Results Overall, 3872 patients were randomized from 39 countries (median age 67 years, 56% female, 56% White, 27% Asian and 10% Black). The median baseline Hb was 9.9 g/dL, blood pressure was 135/74 mmHg and estimated glomerular filtration rate was 18 mL/min/1.73 m2. Among randomized patients, 53% were ESA non-users, 57% had diabetes and 37% had a history of CV disease. At baseline, 61% of participants were using renin–angiotensin system blockers, 55% were taking statins and 49% were taking oral iron. Baseline demographics were similar to those in other large non-dialysis anaemia trials. Conclusion ASCEND-ND will define the efficacy and safety of daprodustat compared with darbepoetin alfa in the treatment of patients with anaemia associated with CKD not on dialysis. [ABSTRACT FROM AUTHOR]

Background: Heart rate variability (HRV) is becoming one of the most valuable tools for assessing a healthy heart's complex and constantly changing oscillations. This study was a pioneering attempt to establish normative data on HRV during treadmill exercise for monitoring the cardiovascular health of the central Indian population. Methodology: This was a cross-sectional study in the Sports Physiology Laboratory of the Department of Physiology in a Rural Medical College in central India. One hundred and twenty healthy subjects in the age range 17-40 years were recruited. Short-term HRV (5 min) was extracted from ECG recordings obtained usingthe Power lab system, AD Instruments, Australia. Results: Time domain indices for males were - Standard deviation of N-N interval (SDNN): 162.61±162.11; Square root of mean squared difference of N-N intervals (RMSSD): 355.79±798.27; the percentage of adjacent NN intervals that differ from each other by more than 50 ms (pNN50): 23.10±27.87. Frequency domain indices in males were-LF power (%)-535.74±3625.96; HF power(%) - 33.15±24.31, LF nu: 33.12± 16.06; HF nu: 57.22±14.89; LF/HF:0.77±0.74. Time-domain indices for females were SDNN as 168.49±130.09; RMSSD: 182.41±154.85; pNN50:32.33±26.59. Frequency domain indicesin females were LF power (%)-19.85±6.13; HF power (%) 43.03±16.39, LF nu-30.53±9.88; HF nu - 60.95±8.70; LF/HF:0.54±0.27. Conclusion: Baseline normative values for HRV spectral and time-domain analysis have been established for their clinical use in comparing the HRV of a healthy individual to that of a deceased individual or an athlete. [ABSTRACT FROM AUTHOR]

Abstract Background Insecticides are currently the main tools used to reduce the transmission of malaria; therefore, the development of resistance to insecticides in malaria vectors is of major concern for malaria control. The resistance level to pyrethroids is particularly high in the Western region of Burkina Faso and may affect the efficacy of insecticidal bed nets and indoor residual spraying. Adult mosquito swarming and other nocturnal behaviours exhibit spatial and temporal patterns that suggest potential vulnerability to targeted space spraying with effective insecticides. Indeed, targeted space-spraying against adult mosquito swarms has been used to crash mosquito populations and disrupt malaria transmission. Methods Prior to impact assessment of swarm killing, a baseline data collection was conducted from June to November 2016 in 10 villages divided into two areas in western Burkina Faso. The data considered both ecological and demographic characteristics to monitor the key entomological parameters. Results The mean number of swarms observed was 35 per village, ranging from 25 to 70 swarms according to the village. Female density in both areas varied significantly as a function of the village and the period of collection. The human biting rate was significantly affected by the period of collection and depended upon whether the collection was carried out indoors or outdoors. Averages of parity rate were high in both areas for all periods of collection, ranging from 60 to 90%. These values ranged from 80 to 100% for inseminated females. Sporozoite rates ranged between 1.6 and 7.2% depending upon the village. The molecular identification of resting and swarming mosquitoes showed the presence of the three major malaria vectors in Burkina Faso, but in different proportions for each village. Conclusions The distribution of the potential swarm markers and swarms in villages suggested that swarms are clustered across space, making intervention easier. Power simulations showed that the direct sampling of swarms provides the highest statistical power, thereby reducing the number of villages needed for a trial.

Abstract Background Using the database of the German Cartilage Registry (KnorpelRegister DGOU), this study aims to present patient- and joint-related baseline data in a large cohort of patients with cam-derived femoroacetabular impingement syndrome (FAI) and to detect symptom-determining factors. Materials and methods Requiring cam morphology as the primary pathology, 362 patients were found to be eligible for inclusion in the study. The assessment of preoperative baseline data was performed using the patient-reported outcome measure—International Hip Outcome Tool (iHOT-33). Descriptive statistics were performed to present baseline data. Univariate and multiple regression with post hoc testing were used to identify patient- and joint-related factors that might affect the preoperative iHOT-33 and its subscores, respectively. Results The study collective’s mean age was 36.71 ± 10.89 years, with 246 (68%) of them being male. The preoperative mean iHOT-33 total was 46.31 ± 20.33 with the subsection “sports and recreational activities” presenting the strongest decline (26.49 ± 20.68). The parameters “age,” “sex,” “body mass index” (BMI), and the confirmation of “previous surgery on the affected hip” were identified to statistically affect the preoperative iHOT-33. In fact, a significantly lower mean baseline score was found in patients aged > 40 years (p 40 years,” “female sex,” “BMI ≥ 25,” and confirmation of “previous surgery on the affected hip” were detected as significantly associated with decreased preoperative iHOT-33 scores. These results help to identify symptom-defining baseline characteristics of cam-derived FAI syndrome. Trial registration: The German Cartilage Registry is conducted in accordance with the Declaration of Helsinki and registered at germanctr.de (DRKS00005617). Registered 3 January 2014—retrospectively registered. The registration of data was approved by the local ethics committees of every participating institution. Primary approval was given by the ethics committee at the University of Freiburg (No. 105/13). https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00005617

Background The A nemia S tudies in chronic kidney disease (C KD): E rythropoiesis via a N ovel prolyl hydroxylase inhibitor D aprodustat- D ialysis (ASCEND-D) trial will test the hypothesis that daprodustat is noninferior to comparator epoetin alfa or darbepoetin alfa for two co-primary endpoints: hemoglobin (Hb) efficacy and cardiovascular (CV) safety. Methods We report the trial design, key demographic, clinical and laboratory findings, and baseline therapies of 2964 patients randomized in the open-label (sponsor-blinded) active-controlled, parallel-group, randomized ASCEND-D clinical trial. We also compare baseline characteristics of ASCEND-D patients with patients who are on dialysis (CKD G5D) enrolled in other large CV outcome trials (CVOTs) and in the most relevant registries. Results The median age of patients was 58 years, 43% were female; 67% were White and 16% were Black. The median Hb at baseline was 10.4 g/dL. Among randomized patients, 89% were receiving hemodialysis and 11% peritoneal dialysis. Among key comorbidities, 42% reported a history of diabetes mellitus and 45% a history of CV disease. Median blood pressure was 134/74 mmHg. The median weekly dose of epoetin was 5751 units. Intravenous and oral iron uses were noted in 64 and 11% of patients, respectively. Baseline demographics were similar to patients with CKD G5D enrolled in other CVOTs and renal patient registries. Conclusions ASCEND-D will evaluate the efficacy and safety of daprodustat compared with epoetin alfa or darbepoetin alfa in the treatment of patients with anemia with CKD G5D. This trial is registered with ClinicalTrials.gov: NCT02879305. EudraCT Number: 2016-000541-31; Sponsor Protocol Number: 200807. [ABSTRACT FROM AUTHOR]

Relevance. The National influenza center uses additional criteria: baselines and thresholds for epidemic intensity for early recognition of the onset and assessment of epidemic intensity. Aim. To characterize the parameters of the flu epidemic in the Russian Federation in the 2019-2020 season and assess the intensity of the last 2 epidemics and the effectiveness of baselines and intensity thresholds. Materials and methods. The database of the research Institute of influenza on weekly morbidity, hospitalization, deaths from influenza and ARVI in the cities-reference bases of the National center for influenza was used. The analysis of the flu situation is based on comparing the current incidence of influenza and ARVI with weekly epic thresholds and baselines. A comparative assessment of the intensity of the last 2 influenza epidemics was carried out using intensity thresholds calculated by the moving epidemic method (MEM). Results. The main parameters of the 2019-20 influenza epidemic compared to the previous one are described. There was an increase in the incidence of clinically diagnosed «fluenza» in the pre-epidemic period and the early onset of both epidemics and reaching a peak in the 6th calendar week. This season, the epidemic started among school children, then among adults, and in the past-among the adult population, the incidence of children 0-2 years old was below the thresholds in both epidemics. This season, the average duration of the epidemic and the incidence in cities were higher among school children and adults, and over the entire period of the epidemic, the incidence was higher in all age groups across the country. This season, the epidemic started in the North Caucasus Federal district, its intensity was medium and low, in the past - it started simultaneously in the Siberian, Ural and North Caucasus districts, and its intensity was very high (in the North Caucasus) and high (in the Siberian Federal district) and the average level of 6 districts. The intensity and duration of epidemics were higher in districts with an early onset (in the North Caucasus and Siberian Federal districts). Both epidemics in the country were of moderate intensity, but there were fewer deaths from influenza in the last epidemic. Conclusion. Comparative characteristics of epidemics using baselines and intensity thresholds allowed us to identify the features of the epidemic process in the seasons 2018-19 and 2019-20: the earlier onset of both epidemics; a noticeable increase in the incidence of fluenza before the beginning of epidemics; a decrease in the intensity of epidemics and mortality from influenza; features of the epidemic process in Federal districts. The effectiveness of using additional criteria in the analysis of the influenza epidemic in Russia and this season has been confirmed. In both epidemics, the weekly epidemic thresholds for influenza and ARVI were more sensitive when determining the start of the epidemic, and the end of it, on the contrary, were baselines, and the baselines of morbidity and hospitalization with a diagnosis «influenza» determined the beginning and end of the epidemic even more precisely.

Large-scale and long-term baselines on climate-sensitive phenology of widespread tree species are lacking in the Indian subcontinent. Citizen scientists can help bridge this information gap by contributing simple, technology-based data. Here we describe an India-wide initiative called SeasonWatch, with preliminary insights into contributor behaviour and species phenology. Between 2011 and 2019, cumulative contributor numbers have increased every year, although consistent contribution remains constant and low. We describe seasonal and spatial phenological patterns in most-observed species based on repeated monitoring and one-time 'bioblitz' events. We study in detail the flowering phenology of one particular species, Cassia fistula, which appears to show aberrant phenology, reflecting a potential shift away from culturally known flowering dates. We conclude that citizen science-contributed information can be a valuable reference database to compare future changes in tree phenology. [ABSTRACT FROM AUTHOR]

Background: COVID-19 protective behaviors are key interventions advised by the World Health Organization (WHO) to prevent COVID-19 transmission. However, achieving compliance with this advice is often challenging, particularly among socially vulnerable groups., Objective: We developed a social vulnerability index (SVI) to predict individuals' propensity to adhere to the WHO advice on protective behaviors against COVID-19 and identify changes in social vulnerability as Omicron evolved in African countries between January 2022 and August 2022 and Asia Pacific countries between August 2021 and June 2022., Methods: In African countries, baseline data were collected from 14 countries (n=15,375) during the first Omicron wave, and follow-up data were collected from 7 countries (n=7179) after the wave. In Asia Pacific countries, baseline data were collected from 14 countries (n=12,866) before the first Omicron wave, and follow-up data were collected from 9 countries (n=8737) after the wave. Countries' socioeconomic and health profiles were retrieved from relevant databases. To construct the SVI for each of the 4 data sets, variables associated with COVID-19 protective behaviors were included in a factor analysis using polychoric correlation with varimax rotation. Influential factors were adjusted for cardinality, summed, and min-max normalized from 0 to 1 (most to least vulnerable). Scores for compliance with the WHO advice were calculated using individuals' self-reported protective behaviors against COVID-19. Multiple linear regression analyses were used to assess the associations between the SVI and scores for compliance to WHO advice to validate the index., Results: In Africa, factors contributing to social vulnerability included literacy and media use, trust in health care workers and government, and country income and infrastructure. In Asia Pacific, social vulnerability was determined by literacy, country income and infrastructure, and population density. The index was associated with compliance with the WHO advice in both time points in African countries but only during the follow-up period in Asia Pacific countries. At baseline, the index values in African countries ranged from 0.00 to 0.31 in 13 countries, with 1 country having an index value of 1.00. The index values in Asia Pacific countries ranged from 0.00 to 0.23 in 12 countries, with 2 countries having index values of 0.79 and 1.00. During the follow-up phase, the index values decreased in 6 of 7 African countries and the 2 most vulnerable Asia Pacific countries. The index values of the least vulnerable countries remained unchanged in both regions., Conclusions: In both regions, significant inequalities in social vulnerability to compliance with WHO advice were observed at baseline, and the gaps became larger after the first Omicron wave. Understanding the dimensions that influence social vulnerability to protective behaviors against COVID-19 may underpin targeted interventions to enhance compliance with WHO recommendations and mitigate the impact of future pandemics among vulnerable groups., (©Suladda Pongutta, Viroj Tangcharoensathien, Kathy Leung, Heidi J Larson, Leesa Lin. Originally published in JMIR Public Health and Surveillance (https://publichealth.jmir.org), 13.08.2024.)

Genetically engineered (GE) crops have the potential to contribute to agricultural sustainability, food security, and nutritional enrichment. However, these crops cannot be released for commercial cultivation without undergoing environmental risk assessments (ERA), thus biosafety evaluation. ERA assessments are performed comparatively with their natural non-GE counterparts. As Bangladesh is progressing with GE potato research, the present study aims to collect baseline information on non-GE potato cultivation with an emphasis on current agronomic practices focusing on fertilizer management and farmers' knowledge base. The survey had three parts, including information on the farmers, information on potato cultivation practices, especially fertilizer use, and lastly, the farmer's view on GE potato. From 2020 to 2021, data were collected through interviews with experienced growers in four potato-growing regions, the Central and Mid-East, North-West, Mid-West, and South-East regions (n = 1757) of the country. The study revealed that farmers of all regions used more than the recommended amounts of fertilizer; for instance, 67.1% more nitrogen fertilizer was applied as an extra dose during potato cultivation in Munshiganj (Central and Mid-East) than in the Dinajpur region (North-West). This overuse of nitrogen fertilizer can enhance plant vigor but makes the plants more susceptible to insect attraction and allows pests easier access to the plants. As a result, the excess dose of nitrogen fertilizer in Munshiganj may act as a catalyst to increase the probability of late blight. The findings also showed that 73.6% of farmers observed unexpected flowering in certain potato cultivars, which corresponded to the higher application of phosphate and potassium fertilizers aimed at late blight control. Furthermore, this study reported infestations of Solanaceous weeds, specifically Solanum torvum and Physalis heterophylla , in potato fields. Finally, our findings demonstrated that more than 68.7% of the potato growers intend to adopt disease-resistant GE potato as that may reduce the need for excess fertilizer use and thus reduce cultivation costs., Competing Interests: Authors AN, SI, MR, MH, TE, MA, and FA were employed by ACI Limited. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor, JR, is currently organizing a research topic with the author AI., (Copyright © 2024 Nahiyan, Islam, Islam, Rahman, Hasan, Eusufzai, Afreen, Ansarey, Khan and Uddin.)

Microplastic pollution has been recognised as a global threat in marine environments and a danger to prey, predators and humans. Yet, there have been few studies in the western Indian Ocean, specifically along the Kenyan coast, which makes it difficult to estimate the extent of such pollution in the region. This is the first study on microplastics (MPs) in the sediments within creeks and estuaries (Tudor, Port Reitz and Mida creeks) on the Kenyan coast. In January/February and September 2018 sediment samples were collected for MPs analysis. The concentration of MPs differed between the sampling seasons and was distinctively higher in the second sampling season across particle sizes, suggesting that there were more MPs from larger terrestrial discharges due to increased runoff. The concentrations of total MPs, and the occurrence of different sizes, shapes and colours, were established under a microscope. The overall mean concentration was highest for the large size category of MPs (500–4 999 µm) at 9.1 (SE 0.8) particles cm−2, with Tudor Creek being more polluted with MPs than Port Reitz and Mida creeks. We recommend formulation of policies on proper plastic waste management and disposal to protect nearshore ecosystems which are rich in marine biodiversity. [ABSTRACT FROM AUTHOR]

Eleven types of petroleum fuels and lubricants including regular gasoline, premium gasoline, jet fuel, kerosene, light oil, bunker A, bunker A–white, bunker A–low sulfur, bunker C, quench oil and lubricant samples were analyzed for parent and alkylated polycyclic aromatic hydrocarbons (PAHs). Naphthalene was the predominant compound in gasolines, jet fuel and kerosene, constituting > 95% of the parent PAHs, whereas dibenzothiophene and other high molecular weight PAHs were predominant in bunker A and bunker C. PAH compositions in petroleum fuels differ because of differences in their refining temperatures and the boiling points of individual PAHs. Principal component analysis classified into four groups of petroleum fuels. Further, oil samples were clearly separated into five groups based on their ratios of select alkyl homologs (C0/(C0+C1) and C4/(C2+C4) naphthalenes): ‘gasolines’ ‘light oil’ ‘bunker oils’ ‘kerosene’ and ‘quench oil’. A wide variety and detailed profiles of PAHs in petroleum fuels and lubricants in this study can be used for baseline data in oil fingerprinting analyses to identify the potential source of oil spill accidents in the environment.

Background: Insecticides are currently the main tools used to reduce the transmission of malaria; therefore, the development of resistance to insecticides in malaria vectors is of major concern for malaria control. The resistance level to pyrethroids is particularly high in the Western region of Burkina Faso and may affect the efficacy of insecticidal bed nets and indoor residual spraying. Adult mosquito swarming and other nocturnal behaviours exhibit spatial and temporal patterns that suggest potential vulnerability to targeted space spraying with effective insecticides. Indeed, targeted space-spraying against adult mosquito swarms has been used to crash mosquito populations and disrupt malaria transmission. Methods: Prior to impact assessment of swarm killing, a baseline data collection was conducted from June to November 2016 in 10 villages divided into two areas in western Burkina Faso. The data considered both ecological and demographic characteristics to monitor the key entomological parameters. Results: The mean number of swarms observed was 35 per village, ranging from 25 to 70 swarms according to the village. Female density in both areas varied significantly as a function of the village and the period of collection. The human biting rate was significantly affected by the period of collection and depended upon whether the collection was carried out indoors or outdoors. Averages of parity rate were high in both areas for all periods of collection, ranging from 60 to 90%. These values ranged from 80 to 100% for inseminated females. Sporozoite rates ranged between 1.6 and 7.2% depending upon the village. The molecular identification of resting and swarming mosquitoes showed the presence of the three major malaria vectors in Burkina Faso, but in different proportions for each village. Conclusions: The distribution of the potential swarm markers and swarms in villages suggested that swarms are clustered across space, making intervention easier. Power simulations showed that the direct sampling of swarms provides the highest statistical power, thereby reducing the number of villages needed for a trial. [ABSTRACT FROM AUTHOR]

Background: Using the database of the German Cartilage Registry (KnorpelRegister DGOU), this study aims to present patient- and joint-related baseline data in a large cohort of patients with cam-derived femoroacetabular impingement syndrome (FAI) and to detect symptom-determining factors.Materials and Methods: Requiring cam morphology as the primary pathology, 362 patients were found to be eligible for inclusion in the study. The assessment of preoperative baseline data was performed using the patient-reported outcome measure-International Hip Outcome Tool (iHOT-33). Descriptive statistics were performed to present baseline data. Univariate and multiple regression with post hoc testing were used to identify patient- and joint-related factors that might affect the preoperative iHOT-33 and its subscores, respectively.Results: The study collective's mean age was 36.71 ± 10.89 years, with 246 (68%) of them being male. The preoperative mean iHOT-33 total was 46.31 ± 20.33 with the subsection "sports and recreational activities" presenting the strongest decline (26.49 ± 20.68). The parameters "age," "sex," "body mass index" (BMI), and the confirmation of "previous surgery on the affected hip" were identified to statistically affect the preoperative iHOT-33. In fact, a significantly lower mean baseline score was found in patients aged > 40 years (p < 0.001), female sex (p < 0.001), BMI ≥ 25 kg/m2 (p = 0.002) and in patients with previous surgery on the affected hip (p = 0.022). In contrast, the parameters defect grade and size, labral tears, and symptom duration delivered no significant results.Conclusions: A distinct reduction in the baseline iHOT-33, with mean total scores being more than halved, was revealed. The parameters "age > 40 years," "female sex," "BMI ≥ 25," and confirmation of "previous surgery on the affected hip" were detected as significantly associated with decreased preoperative iHOT-33 scores. These results help to identify symptom-defining baseline characteristics of cam-derived FAI syndrome.Trial Registration: The German Cartilage Registry is conducted in accordance with the Declaration of Helsinki and registered at germanctr.de (DRKS00005617). Registered 3 January 2014-retrospectively registered. The registration of data was approved by the local ethics committees of every participating institution. Primary approval was given by the ethics committee at the University of Freiburg (No. 105/13). https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00005617. [ABSTRACT FROM AUTHOR]

Worldwide, states are gazetting new Marine Protected Areas (MPAs) to meet the international commitment of protecting 30% of the seas by 2030. Yet, protection benefits only come into effect when an MPA is implemented with activated regulations and actively managed through continuous monitoring and adaptive management. To assess if actively managed MPAs are the rule or the exception, we used the Mediterranean and Black Seas as a case study, and retrieved information on monitoring activities for 878 designated MPAs in ten European Union (EU) countries. We searched for scientific and grey literature that provides information on the following aspects of MPA assessment and monitoring: ecological (e.g., biomass of commercially exploited fish), social (e.g., perceptions of fishers in an MPA), economic (e.g., revenue of fishers) and governance (e.g., type of governance scheme). We also queried MPA authorities on their past and current monitoring activities using a web-based survey through which we collected 123 responses. Combining the literature review and survey results, we found that approximately 16% of the MPA designations (N = 878) have baseline and/or monitoring studies. Most monitoring programs evaluated MPAs based solely on biological/ecological variables and fewer included social, economic and/or governance variables, failing to capture and assess the social-ecological dimension of marine conservation. To increase the capacity of MPAs to design and implement effective social-ecological monitoring programs, we recommend strategies revolving around three pillars: funding, collaboration, and technology. Following the actionable recommendations presented herein, MPA authorities and EU Member States could improve the low level of MPA monitoring to more effectively reach the 30% protection target delivering benefits for biodiversity conservation. [Display omitted] • Protection benefits occur only when an MPA is implemented and actively managed. • Literature and surveys were used to assess actively managed MPAs in Southern Europe. • Barely 16% of designated MPAs have baseline and/or monitoring studies. • Funding, technology, and collaboration strategies could increase MPAs' efficiency. [ABSTRACT FROM AUTHOR]

The activity concentrations of the natural radionuclides, namely226Ra,232Th, and40K were measured from soil samples collected from different locations of Egbeda Local Government Area, Oyo state, Nigeria, with the aim of establishing radioactivity baseline data for the area. High-resolution gamma spectrometry detector was used to determine the activity concentration of these radionuclides in 45 soil samples taken at a depth of about 15 cm in various communities of Egbeda Local Government Area. The concentration of226Ra was in the range 5.9–61.3 Bq/kg with an average value of 30.5 Bq/kg,232Th in the range 4.0–116.5 Bq/kg with an average value of 50.8 Bq/kg, and40K in the range 27–798 Bq/kg with an average value of 200 Bq/kg. The results obtained in this study were compared with those of different countries in the world. Radiological index parameters were used for the assessment of radiological exposure of the natural radioactivity, and the absorbed dose rate, the radium equivalent activity, the annual effective dose, the external exposure index, and internal exposure index were calculated. There is no radiological risk from soil that may threat the residents around Egbeda Local Government Area. Hence, the probability of occurrence of any of the health effects of radiation is insignificant. Therefore, the radioactivity measurements taken represent the baseline data of the study area.

AimsTo characterize the genetic landscape and mutation spectrum of patients with corneal dystrophies (CDs) in a large Han ethnic Chinese Cohort with inherited eye diseases (IEDs).MethodsRetrospective study. A large IED cohort was recruited in this study, including 69 clinically diagnosed CD patients, as well as other types of eye diseases patients and healthy family members as controls. The 792 genes on the Target_Eye_792_V2 chip were used to screen all common IEDs in our studies, including 22 CD-related genes.ResultsWe identified 2334 distinct high-quality variants on 22 CD-related genes in a large IEDs cohort. A total of 21 distinct pathogenic or likely pathogenic mutations were identified, and the remaining 2313 variants in our IED cohort had no evidence of CD-related pathogenicity. Overall, 81.16% (n = 56/69) of CD patients received definite molecular diagnoses, and transforming growth factor-beta-induced protein (TGFBI), CHTS6, and SLC4A11 genes covered 91.07, 7.14, and 1.79% of the diagnosed cases, respectively. Twelve distinct disease-associated mutations in the TGFBI gene were identified, 11 of which were previously reported and one is novel. Four of these TGFBI mutations (p.D123H, p.M502V, p.P501T, and p.P501A) were redefined as likely benign in our Han ethnic Chinese IED cohort after performing clinical variant interpretation. These four TGFBI mutations were detected in asymptomatic individuals but not in CD patients, especially the previously reported disease-causing mutation p.P501T. Among 56 CD patients with positive detected mutations, the recurrent TGFBI mutations were p.R124H, p.R555W, p.R124C, p.R555Q, and p.R124L, and the proportions were 32.14, 19.64, 14.29, 10.71, and 3.57%, respectively. Twelve distinct pathogenic or likely pathogenic mutations of CHTS6 were detected in 28 individuals. The recurrent mutations were p.Y358H, p.R140X, and p.R205W, and the proportions were 25.00, 21.43, and 14.29%, respectively. All individuals associated with TGFBI were missense mutations; 74.19% associated with CHTS6 mutations were missense mutations, and 25.81% were non-sense mutations. Hot regions were located in exons 4 and 12 of TGFBI individuals and located in exon 3 of CHTS6 individuals. No de novo mutations were identified.ConclusionFor the first time, our large cohort study systematically described the variation spectrum of 22 CD-related genes and evaluated the frequency and pathogenicity of all 2334 distinct high-quality variants in our IED cohort. Our research will provide East Asia and other populations with baseline data from a Han ethnic population-specific level.

To assess the seasonal change of seabird abundance in the Tsugaru Strait, which is designated as a Marine Important Bird Area (IBA), we conducted surveys between Hakodate (Hokkaido) and Oma (Aomori) during 151 regular ferry transits from October 2006 to November 2010. We observed and recorded 51 species from 11 families of seabirds. The density of seabirds was highest during the spring migration period (April to May). In that period, the abundance of migrants such as Procellariidae (mainly Shorttailed Shearwaters Puffinus tenuirostris and Sooty Shearwaters P. griseus) and Red-necked Phalarope Phalaropus lobatus was highest. The species richness was highest during the late autumn to early wintering period (33 species in November and December). The Laridae (mainly Black-legged Kittiwake Rissa tridactyla and Mew Gull Larus canus) and Alcidae (mainly Rhinoceros Auklet Cerorhinca monocerata and Ancient Murrelet Synthliboramphus antiquus) increased during that winter season. Our results show the variety and seasonal abundance of various coastal and pelagic seabirds in the Tsugaru Strait. [ABSTRACT FROM AUTHOR]

Background: Mucopolysaccharidoses (MPS) are a group of lysosomal storage disorders, leading to the progressive accumulation of glycosaminoglycans (GAGs) and the subsequent compromising of tissues and organ malfunction. Although incurable, most types of MPS can be treated with enzyme replacement therapy (ERT), an approach that has had positive effects on the natural clinical evolution and which impact has been extensively investigated. Unfortunately, to date, there is relatively little data regarding the effects of ERT interruption, especially in Latin America, where such interruption may be frequent due to a variety of issues (for instance, difficulties involving logistics, reimbursement and/or payment withdrawal). Method: A group of medical professionals from Latin America with experience in Genetics, Pediatrics and Neurology held an Advisory Board Meeting in the city of São Paulo, in October 2018, to discuss the issue of ERT interruptions in the region and recommendations to health care professionals on how to deal with these interruptions and better assess the therapeutic effects of ERT. Conclusion: Recommendations provided by the experts may support physicians in dealing with the most common reasons for ERT interruptions in Latin America. Most importantly, recommendations for data collection at specific timepoints (at baseline, throughout the treatment and during the interruption period of ERT and after its resumption) can significantly improve the collection of real world evidence on the effects of ERT and its interruptions, supporting health care professionals and policy makers in the decision making regarding the provision of these therapies for MPS patients.

Baseline data represent one of the important stages of Environmental Impact Assessment (EIA) procedure that describes the existing environment of the study area and surrounding areas in enough detail to allow the environmental impacts of the proposed area to be accurately and adequately assessed, and future changes and effects can be measured. Baseline data may be inaccurate, difficult to obtain or non-existent in Iran as a developing country, and it involves huge and diverse environmental data of a spatial nature in the EIA process. Therefore, a useful and effective geographic information system (GIS) approach is developed to integrate geodatabase by acquiring and handling environmental data from different sources related to the proposed project area for the EIA procedure. Based on this approach, a comprehensive geodatabase for baseline data of EIA procedure, called Baseline Geodatabase (BGDB), is designed. The BGDB includes feature datasets (projects, climatology and air quality, hydrology, pedology, general location map of the study area, geology, noise, biology, and socio-economic-cultural data) and tables (fauna). For example, the BGDB for Sabalan geothermal project area, Iran as a case study is developed. This paper provides a practical tool to facilitate the EIA process, environmentally sustainable management, and to support decision-making for environmental specialists and managers in the present and future of the proposed project area.

Many large predatory fishes are in decline and tuna sustainability is high on the global agenda. Slender tuna (SLT), Allothunnus fallai, is data‐poor and a rare contemporary example of a globally unexploited temperate tuna. This study analysed 214 otoliths for age and growth of fish collected in the South Atlantic. Observed ages varied between 9 and 42 years for a size range of 68–90 cm fork length. We reveal important life history data for SLT before exploitation and underline the relevance of data‐poor stocks in understanding wider questions for exploited tuna. [ABSTRACT FROM AUTHOR]

Spatial analyses of marine mammal stranding data can be used to identify stranding patterns and improve surveillance and monitoring. Using ArcGIS and SaTScan, we analyzed 12 years (2002–2014) of dead beachcast marine mammals from San Juan County, Washington, to better understand patterns of carcass deposition. We plotted the locations for 631 dead marine mammals and aggregated strandings into 1,000 m segments of shoreline. "Hot spots" included beach segments with significantly higher carcass deposition according to the Getis‐Ord Gi* statistic in ArcGIS or beach segments that were encompassed by significant spatial clusters using the discrete Poisson model in SatTScan. We identified 52 hot spots for harbor seals (Phoca vitulina) in ArcGIS and 62 hot spots in SaTScan with 81% agreement between methods. Carcass deposition showed a strong relationship with locations having high proximity to public pedestrian access points, suggesting increased reporting effort in those areas. Stranding frequency was also higher on beach segments with longer fetch and nearly level or gentle slopes. Beaches with these geomorphic characteristics, especially those without high proximity to public access, would be ideal locations to actively survey to improve high quality carcass collection during times of high expected mortality and limited resources. [ABSTRACT FROM AUTHOR]

Objective: Data on preoperative distress and health-related quality-of-life (HRQoL) is lacking for patients with newly diagnosed renal tumors. This study aims to compare HRQoL within this group with the general population and to study the relationship between distress, HRQoL, personality, coping, and patient/tumor-related factors. Materials and methods: Between January 2011 and June 2014, 153 patients (100 males/53 females), scheduled for surgery were prospectively included. Distress was determined by the General Health Questionnaire (GHQ), HRQoL by EORTC-QLQ-C30 questionnaire, personality by Eysenck Personality Inventory and coping by COPE questionnaire. HRQoL-data from an age and gender matched Norwegian reference population was used for comparison. Results: The study patients had significantly poorer HRQoL than the reference population. GHQ and HRQoL sum scores had a common variance (CV = r2) of 29–35%. In regression models, the measured variables accounted for 33% of the variance for the GHQ score. Significant predictors of the measured variance were neuroticism (18%), education level (3%) and avoidant coping (2%). Similarly, the measured variables accounted for 33–44% of the variance for the HRQoL sum scores. For all HRQoL sum scores, neuroticism predicted 17–28%, while education predicted 4–11% of the measured variance. Large tumor size, comorbidity, performance status and CRP predicted 2–7% of individual sum scores. Conclusions: For both preoperative distress and HRQoL, personality traits such as neuroticism and education level were the most important predictors. Tumor-related factors and other preexisting conditions seemed to be of lesser importance. Thus, preoperatively screening of psychological factors could be helpful to identify those at risk of poor outcomes. [ABSTRACT FROM AUTHOR]

Introduction: The API AutosomalDominant AD (ADAD) Colombia Trial is a placebo‐controlled clinical trial of crenezumab in 252 cognitively unimpaired 30 to 60‐year‐old Presenilin 1 (PSEN1) E280A kindred members, including mutation carriers randomized to active treatment or placebo and non‐carriers who receive placebo. Methods: Of the 252 enrolled, we present data on a total of 242 mutation carriers and non‐carriers matched by age range, excluding data on 10 participants to protect participant confidentiality, genetic status, and trial integrity. Results: We summarize demographic, clinical, cognitive, and behavioral data from 167 mutation carriers and 75 non‐carriers, 30 to 53 years of age. Carriers were significantly younger than non‐carriers ((mean age ± SD) 37 ± 5 vs 42 ± 6), had significantly lower Mini Mental Status Exam (MMSE) scores (28.8 ± 1.4 vs 29.2 ± 1.0), and had consistently lower memory scores. Discussion: Although PSEN1 E280A mutation carriers in the Trial are cognitively unimpaired, they have slightly lower MMSE and memory scores than non‐carriers. Their demographic characteristics are representative of the local population. [ABSTRACT FROM AUTHOR]

Background/Aims: Published methods for sample size calculation for cluster randomised trials with baseline data are inflexible and primarily assume an equal amount of data collected at baseline and endline, that is, before and after the intervention has been implemented in some clusters. We extend these methods to any amount of baseline and endline data. We explain how to explore sample size for a trial if some baseline data from the trial clusters have already been collected as part of a separate study. Where such data aren't available, we show how to choose the proportion of data collection devoted to the baseline within the trial, when a particular cluster size or range of cluster sizes is proposed. Methods: We provide a design effect given the cluster size and correlation parameters, assuming different participants are assessed at baseline and endline in the same clusters. We show how to produce plots to identify the impact of varying the amount of baseline data accounting for the inevitable uncertainty in the cluster autocorrelation. We illustrate the methodology using an example trial. Results: Baseline data provide more power, or allow a greater reduction in trial size, with greater values of the cluster size, intracluster correlation and cluster autocorrelation. Conclusion: Investigators should think carefully before collecting baseline data in a cluster randomised trial if this is at the expense of endline data. In some scenarios, this will increase the sample size required to achieve given power and precision. [ABSTRACT FROM AUTHOR]

Salmonella enterica serovar Schwarzengrund is one of the most frequently isolated Salmonella serotypes responsible for human and poultry infections in Taiwan, and it has raised public health concerns. To better facilitate the understanding of transmission patterns and the dynamics of epidemics, sharing molecular data on pathogen profiles is urgently needed. The objectives of the current study were to determine and establish baseline data of S. enterica serovar Schwarzengrund isolates from 23 epidemiologically unrelated sources from year 2000 to 2018 and examine their phenotypic and genotypic characteristics. Genomic DNA of the Salmonella isolates was extracted and subjected to whole-genome sequencing using an Illumina platform. Results showed that all selected isolates exhibited multidrug resistance, and six of those were resistant to ciprofloxacin phenotypically. Genotypically, these isolates carried genes resistant to aminoglycoside (100%), phenicol (91.3%), β-lactams (69.5%), folate pathway antagonist (100%), tetracycline (82.6%), and fluoroquinolone (4.3%). Moreover, these isolates harbor integrons with five different gene cassettes identified for the first time, which are associated with resistance to trimethoprim, streptomycin, tetracycline, sulfonamide, chloramphenicol, and gentamicin. Furthermore, prevalence of IncFIB plasmid was found among studied isolates, which may increase its ability to colonize the chicken cecum and cause extra-intestinal disease. Salmonella pathogenicity islands SPI-1 to SPI-5, SPI-13, and SPI-14, as well as C63PI locus, were also detected in all isolates. This study demonstrated that a considerable high antimicrobial resistance with high virulence levels of Salmonella were found from animal sources. Sharing data on these pathogen profiles can not only help increase the reproducibility and accessibility of genomic analysis but can also support surveillance and epidemiological investigations for salmonellosis in the region.