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4. Search for the genes involved in oocyte maturation and early embryo development in the hen

Author

Blesbois Elisabeth, Monget Philippe, Martin-Magniette Marie-Laure, Balzergue Sandrine, Couty Isabelle, Batellier Florence, Elis Sebastien, and Govoroun Marina S

Subjects
Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background The initial stages of development depend on mRNA and proteins accumulated in the oocyte, and during these stages, certain genes are essential for fertilization, first cleavage and embryonic genome activation. The aim of this study was first to search for avian oocyte-specific genes using an in silico and a microarray approaches, then to investigate the temporal and spatial dynamics of the expression of some of these genes during follicular maturation and early embryogenesis. Results The in silico approach allowed us to identify 18 chicken homologs of mouse potential oocyte genes found by digital differential display. Using the chicken Affymetrix microarray, we identified 461 genes overexpressed in granulosa cells (GCs) and 250 genes overexpressed in the germinal disc (GD) of the hen oocyte. Six genes were identified using both in silico and microarray approaches. Based on GO annotations, GC and GD genes were differentially involved in biological processes, reflecting different physiological destinations of these two cell layers. Finally we studied the spatial and temporal dynamics of the expression of 21 chicken genes. According to their expression patterns all these genes are involved in different stages of final follicular maturation and/or early embryogenesis in the chicken. Among them, 8 genes (btg4, chkmos, wee, zpA, dazL, cvh, zar1 and ktfn) were preferentially expressed in the maturing occyte and cvh, zar1 and ktfn were also highly expressed in the early embryo. Conclusion We showed that in silico and Affymetrix microarray approaches were relevant and complementary in order to find new avian genes potentially involved in oocyte maturation and/or early embryo development, and allowed the discovery of new potential chicken mature oocyte and chicken granulosa cell markers for future studies. Moreover, detailed study of the expression of some of these genes revealed promising candidates for maternal effect genes in the chicken. Finally, the finding concerning the different state of rRNA compared to that of mRNA during the postovulatory period shed light on some mechanisms through which oocyte to embryo transition occurs in the hen.

Published
2008
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5. Transcriptomic approach to study oocyte competence in the hen

Author

Elis, Sébastien, Blesbois, Elisabeth, Dupont, Joëlle, Monget, Philippe, Couty, Isabelle, Balzergue, Sandrine, Martin-Magniette, Marie-Laure, Batellier, Florence, Govoroun, Marina, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), AgroParisTech, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
MATURATION FOLLICULAIRE, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences, MICROARRAY, GENETIQUE ANIMALE, DEVELOPPEMENT PRECOCE DE L'EMBRYON, ComputingMilieux_MISCELLANEOUS, INDICE DE FERTILITE
Abstract

Program & Book of Abstracts Communication orale S2 Texte intégral de 8 pages sur CDROM; National audience

Published
2010

6. Mise au point d'un milieu pour la cryoconservation de la semence équine et mécanismes de cryoprotection impliqués

Author

Pillet, Elodie, Batellier, Florence, Magistrini, Michèle, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], MILIEU DE CONGELATION, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], MILIEU INRA96, [INFO] Computer Science [cs]
Abstract

Actualités en élevage équin; National audience

Published
2010

7. Method for preserving sperm and applications thereof

Author

Magistrini, Michèle, Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], A01N1/02, A61K35/52, C12N5/02, A61K35/48
Abstract

Demande de dépôt de brevet le 27 décembre 2007 Fr-07 09145. Demande Internationale n°PCT/FR2008/001822 du 24 décembre 2008

Published
2009

8. A new freezing extender for stallion semen to get high fertility rates

Author

Pillet, Elodie, Duchamp, Guy, Batellier, Florence, Yvon, Jean-Marie, Delhomme, G., Desherces, S., Schmitt, E., Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), IMV Technologies, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], INRA96, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], INRA82, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2009

9. Vers la mise au point d'un nouveau milieu de congélation pour la semence équine : le plasma de jaune d'oeuf gamma-irradié peut remplacer le jaune d'oeuf

Author

Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Bruneau, Bernard, Yvon, Jean-Marie, Desherces, S., Schmitt, E., Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), IMV Technologies, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], CONGELATION DU SPERME, [SDV]Life Sciences [q-bio], MILIEU, ETALON, JAUNE D'OEUF, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, CRYOPROTECTION
Abstract

National audience

Published
2009

10. Evaluation de la qualité des spermatozoïdes d'étalons pur sang Arabe congelés dans le milieu INRA 96® supplémenté de jaune d'oeuf et de glycérol

Author

Najjar, A., Yvon, Jean-Marie, Benaoun, B., Ezzaouia, M., Pillet, Elodie, Batellier, Florence, Ben M'Rad, Moncef, Magistrini, Michèle, Institut National de la Recherche Agronomique de Tunisie (INRAT), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Haras National de Sidi Thabet, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], CONGELATION, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], PARAMETRES IN VITRO, PUR SANG ARABE, ComputingMilieux_MISCELLANEOUS, MILIEU INRA 96
Abstract

National audience

Published
2009

11. Identification of germinal disk region derived genes potentially involved in hen fertility

Author

Elis, Sébastien, Blesbois, Elisabeth, Couty, Isabelle, Balzergue, Sandrine, Martin-Magniette, Marie-Laure, Batellier, Florence, Govoroun, Marina, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
POULET A VIANDE, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Abstract

International audience; Despite the regular decrease in fertility observed in hens, especially in "meat" lines, little is known about genes affecting fertility. We used the Affymetrix microarray to search for oocyte genes whose expression would vary in relation to fertility rate in both "laying" and "meat" line hens. We focused on oocyte genes because several of them have been found to be involved in fertility in other species. Based on microarray analysis, 54 and 84 genes were differentially expressed between germinal disc regions (GDR) of F1 maturation stage oocytes from hens exhibiting either high (100%) or low (from 22% to 80%) fertility rate from laying and meat lines respectively. Most of these differentially expressed genes were distributed between "laying" and,.meat" lines indicating that mechanisms involved in the decrease in fertility rates in these two cases were independent. Real time RT-PCR performed on the same samples which were used for microarray confirmed in several cases differences in gene expression levels detected by microarray. Moreover the correlations between gene expression levels and fertility rates were evaluated for the 10 most interesting genes at different stages of follicular maturation and early embryo development on individual GDR samples from hens exhibiting different fertility rates. In total, we identified five genes whose expression levels correlated with fertility rate in accordance with findings of microarray analysis and real time RT-PCR: VWC2, CR407412, TAPA, FGL2, and TRAP6. The biological significance of these genes sheds light on potential mechanisms influencing fertility and could provide candidates for fertility markers in the hen.

Published
2009
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12. Le milieu INRA96® améliore la fertilité de la semence congelée d'étalon

Author

Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Le Vern, Yves, Kerboeuf, Dominique, Desherces, S., Schmitt, E., Vidament, Marianne, Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique (UR IASP), IMV Technologies, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], MILIEU DE CONGELATION, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], MILIEU INRA96®, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2008

13. Du nouveau pour la congélation de la semence d'étalon

Author

Pillet, Elodie, Batellier, Florence, Magistrini, Michèle, Duchamp, Guy, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], MILIEU INRA96, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2008

14. Le milieu INRA96® supplémenté de jaune d'oeuf et de glycérol améliore la fertilité en insémination artificielle de semence congelée

Author

Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Furstoss, Vincent, Bruneau, Bernard, Yvon, Jean-Marie, Le Vern, Yves, Kerboeuf, Dominique, Vidament, Marianne, Magistrini, Michèle, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), Infectiologie Animale et Santé Publique (UR IASP), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], CONGELATION, FERTILITE, [SDV]Life Sciences [q-bio], ETALON, [INFO]Computer Science [cs], MILIEU INRA96, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2008

15. Inra96® : un milieu efficace pour congeler la semence d'étalon

Author

Batellier, Florence, Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], MILIEU INRA96, [INFO]Computer Science [cs], [INFO] Computer Science [cs]
Abstract

"en direct des labos", la lettre électronique de l'Inra pour les entreprises; National audience

Published
2008

16. Hen oocyte specificity and genes expression during early embryo development

Author

Elis, Sébastien, Govoroun, Marina, Monget, Philippe, Couty, Isabelle, Blesbois, Elisabeth, Batellier, Florence, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2007

17. Hen oocyte quality and fertility

Author

Elis, Sébastien, Govoroun, Marina, Dupont, Joëlle, Monget, Philippe, Couty, Isabelle, Blesbois, Elisabeth, Batellier, Florence, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Biotechnology and Biological Sciences Research Council (BBSRC). GBR., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
fertilité, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], expression génique, qualité, maturation de l'ovocyte, poule, ComputingMilieux_MISCELLANEOUS, Autre (Sciences du Vivant)
Abstract

International audience

Published
2007

18. Compétence ovocytaire chez la poule

Author

Elis, Sébastien, Govoroun, Marina, Monget, Philippe, Couty, Isabelle, Brillard, Jean-Pierre, Blesbois, Elisabeth, Batellier, Florence, ProdInra, Migration, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

19. Qualité des gamètes chez le poulet, recherche de gènes candidats

Author

Elis, Sébastien, Batellier, Florence, Govoroun, Marina, Monget, Philippe, Blesbois, Elisabeth, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), AGENAE - Analyse du Génome des Animaux d'Elevage (AGENAE). FRA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], expression génique, qualité des gamètes, gène, poulet, gallus gallus, ovocyte, séquence du génome, fertilité, transcriptome, gène candidat, ComputingMilieux_MISCELLANEOUS, Autre (Sciences du Vivant)
Abstract

National audience

Published
2006

20. Identification, purification et mécanisme d'action d'éléments contenus dans le lait, agissant sur les spermatozoïdes équins

Author

Méa-Batellier, Florence, ProdInra, Migration, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), and Université François Rabelais (Tours)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ALPHA-LACTALBUMINE, [INFO]Computer Science [cs], these, [INFO] Computer Science [cs]
Abstract

*INRA Tours Unité de la Reproduction et des Comportements, INRA Tours Diffusion du document : INRA Tours Unité de la Reproduction et des Comportements, INRA Tours Diplôme : Dr. d'Université

Published
1997

24. Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Author

Govoroun, Marina S., Pannetier, Maëlle, Pailhoux, Eric, Cocquet, Julie, Brillard, Jean‐Pierre, Couty, Isabelle, Batellier, Florence, and Cotinot, Corinne

Abstract

Mutations in the forkhead transcription factor gene FOXL2 are involved in ovarian failure, which occurs in human BPES syndrome. This syndrome presents a sexually dimorphic expression, specific to the ovary in several vertebrates. We cloned the open reading frame of chicken FOXL2 (cFoxL2) and studied cFoxL2 expression in developing gonads and during adulthood to examine the role of FOXL2 in ovarian differentiation and function in birds. The spatial and temporal dynamics of cFoxL2 and aromatase expression were analyzed in parallel by using real‐time quantitative reverse transcriptase‐polymerase chain reaction and immunohistochemistry in attempt to investigate the possible role of cFoxL2 in the regulation of aromatase. The expression patterns of cFoxL2 and aromatase transcripts were highly correlated during the sex‐differentiation period (4.7–12.7 days of incubation). Aromatase and cFoxL2 proteins were colocalized in the medullar part of female gonads on embryonic day 14. Fourteen days after hatching, cFoxL2 protein was mainly detected in granulosa cells of developing follicles. In adult ovary follicular envelopes, apart from granulosa cells, cFoxL2 transcript and protein were detected at lower levels in theca cells where aromatase was present. A high level of cFoxL2 transcription was also observed in maturing and ovulated oocytes. Our results confirm that FoxL2 is an early regulator of ovarian development in birds and may be involved in aromatase transcription regulation. Developmental Dynamics 859–870, 2004. © 2004 Wiley‐Liss, Inc.

Published
2004
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25. Identification of germinal disk region derived genes potentially involved in hen fertility.

Author

Elis S, Blesbois E, Couty I, Balzergue S, Martin-Magniette ML, Batellier F, and Govoroun MS

Subjects
Analysis of Variance, Animal Husbandry, Animals, Blastodisc embryology, Chick Embryo, Chickens growth & development, Oligonucleotide Array Sequence Analysis methods, Oocytes growth & development, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Blastodisc physiology, Chickens genetics, Fertility genetics, Gene Expression Profiling methods, Gene Expression Regulation, Developmental
Abstract

Despite the regular decrease in fertility observed in hens, especially in "meat" lines, little is known about genes affecting fertility. We used the Affymetrix microarray to search for oocyte genes whose expression would vary in relation to fertility rate in both "laying" and "meat" line hens. We focused on oocyte genes because several of them have been found to be involved in fertility in other species. Based on microarray analysis, 54 and 84 genes were differentially expressed between germinal disc regions (GDR) of F1 maturation stage oocytes from hens exhibiting either high (100%) or low (from 22% to 80%) fertility rate from laying and meat lines respectively. Most of these differentially expressed genes were distributed between "laying" and "meat" lines indicating that mechanisms involved in the decrease in fertility rates in these two cases were independent. Real time RT-PCR performed on the same samples which were used for microarray confirmed in several cases differences in gene expression levels detected by microarray. Moreover the correlations between gene expression levels and fertility rates were evaluated for the 10 most interesting genes at different stages of follicular maturation and early embryo development on individual GDR samples from hens exhibiting different fertility rates. In total, we identified five genes whose expression levels correlated with fertility rate in accordance with findings of microarray analysis and real time RT-PCR: VWC2, CR407412, TAPA, FGL2, and TRAP6. The biological significance of these genes sheds light on potential mechanisms influencing fertility and could provide candidates for fertility markers in the hen.

Published
2009
Full Text
View/download PDF

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