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1. Purification and characterization of a protease produced by submerged fermentation: Ultrasound-enhanced collagenolytic protease from Streptomyces parvulus

Author

Silva, Raphael Luiz Andrade, Barreiro, Maria Ercilia, Ferreira, Kétura Rhammá Cavalcante, Cardoso, Kethylen Barbara Barbosa, Neves, Anna Gabrielly Duarte, Miranda, Maria Eduarda L.C., Batista, Juanize Matias, Nascimento, Thiago Pajeú, Cahú, Thiago Barbosa, de Souza Bezerra, Ranilson, Porto, Ana Lucia F., and Brandão-Costa, Romero M.P.

Published
2024
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2. Role of gamma radiation as an agent modulator of Mucor subtilissimus UCP1262 Fibrinolytic Enzyme (MsFE)

Author

Cardoso, Kethylen Barbara Barbosa, Nascimento, Thiago Pajeú, Correia, Patyanne Carvalho, da Silva Batista, Juanize Matias, Brandão-Costa, Romero Marcos Pedrosa, Ferreira, Kétura Rhammá Cavalcante, de Carvalho-Silva, Jônatas, da Cruz Augusto Neves Petersen, Maria Teresa, Albuquerque, Wendell, Salazar e Fernandes, Thiago de, Converti, Attilio, and Porto, Ana Lúcia Figueiredo

Published
2024
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3. ENZIMAS FIBRINOLÍTICAS MICROBIANAS COMO ABORDAGEM PROMISSORA PARA DISTÚRBIOS TROMBOEMBÓLICOS

Author

Cardoso, Kethylen Barbara Barbosa, primary, Nascimento, Maria Clara do, additional, Silva, Celso Lucas Gomes da, additional, Melo, Diva Maria Izabel de Oliveira Silva, additional, Ferreira, Kétura Rhammá Cavalcante, additional, Batista, Juanize Matias da Silva, additional, Nascimento, Thiago Pajeú, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2023
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4. ESTUDOS in sílico SOBRE A INTERAÇÃO DE INIBIDORES DE ORIGEM VEGETAL COM O MODELO 6lu7 DA PROTEASE MPRO DO CORONAVÍRUS: UMA MINI REVISÃO

Author

Nascimento, Maria Clara do, primary, Ferreira, Julyanne Victória dos Santos, additional, Silva, Anna Carolina Batista e, additional, Batista, Juanize Matias da Silva, additional, Nascimento, Thiago Pajeú, additional, Porto, Ana Lúcia Figueiredo, additional, and Costa, Romero Marcos Pedrosa Brandão, additional

Published
2023
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5. LECTINAS MICROBIANAS: PERSPECTIVAS NA SAÚDE E TERAPIAS INOVADORAS

Author

Nascimento, Maria Clara do, primary, Pereira, Ana Sofia Faria, additional, Freitas, Pedro Melo Cordeiro de, additional, Silva, Nilson Fernando Barbosa da, additional, Batista, Juanize Matias da Silva, additional, Nascimento, Thiago Pajeú, additional, Porto, Ana Lúcia Figueiredo, additional, and Costa, Romero Marcos Brandão da, additional

Published
2023
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7. MÉTODOS DE SEPARAÇÃO E PURIFICAÇÃO DE IMUNOGLOBULINA G: UMA BREVE REVISÃO

Author

Silva, Iasmin Marcela Siqueira da, primary, Barbosa Filho, José Pedro Martins, additional, Batista, Juanize Matias da Silva, additional, Cavalcante Junior, Josias Pereira, additional, Campos, Thiers Araújo, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Porto, Ana Lúcia Figueiredo, additional, Couto, Milena Tereza Torres do, additional, Sobral, Renata Vitória da Silva, additional, Nascimento, Thiago Pajeú, additional, and Leite, Ana Cristina Lima, additional

Published
2022
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8. BIOTECNOLOGIA FARMACÊUTICA-VOL 1

Author

Porto, Ana Lúcia Figueiredo, primary, Santana, Beatriz de Fátima Maia de, additional, Marques, Daniela de Araújo Viana, additional, Barbosa Filho, José Pedro Martins, additional, Batista, Juanize Matias da Silva, additional, Cardoso, Kethylen Barbara Barbosa, additional, Silva, Maria Rafaele Oliveira Bezerra da, additional, Costa, Romero Marcos Pedrosa Brandão, additional, and Nascimento, Thiago Pajeú, additional

Published
2022
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9. BIOTECNOLOGIA NA SAÚDE-VOL 2

Author

Porto, Ana Lúcia Figueiredo, primary, Marques, Daniela de Araújo Viana, additional, Barbosa Filho, José Pedro Martins, additional, Batista, Juanize Matias da Silva, additional, Cardoso, Kethylen Barbara Barbosa, additional, Silva, Maria Rafaele Oliveira Bezerra da, additional, Nascimento, Maria Clara do, additional, Costa, Romero Marcos Pedrosa Brandão, additional, and Nascimento, Thiago Pajeú, additional

Published
2022
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11. Biotechnological purification of a β-fructofuranosidase (β-FFase) from Aspergillus tamarii kita: Aqueous two-phase system (PEG/Citrate) and biochemical characterization

Author

Matias da Silva Batista, Juanize, Pedrosa Brandão-Costa, Romero Marcos, Barbosa Cardoso, Kethylen Barbara, Nascimento, Thiago Pajeú, Albuquerque, Wendell W.C., Carneiro da Cunha, Márcia Nieves, Porto, Camila Souza, Bezerra, Raquel Pedrosa, and Figueiredo Porto, Ana Lucia

Published
2021
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13. PRINCIPAIS SUPORTES DE IMOBILIZAÇÃO DE PROTEASES

Author

Regueira, Bruno Vinícius Barros, primary, Couto, Milena Tereza Torres do, additional, Silva, Aníbia Vicente da, additional, Leite, Ana Cristina Lima, additional, Silva, Marllyn Marques da, additional, Oliveira, Vagne de Melo, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Batista, Juanize Matias da Silva, additional, Porto, Ana Lúcia Figueiredo, additional, and Nascimento, Thiago Pajeú, additional

Published
2022
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14. BIOTECNOLOGIA AMBIENTAL

Author

Oliveira, Vagne de Melo de, primary, Nascimento, Thiago Pajeú, additional, Batista, Juanize Matias da Silva, additional, Cardoso, Kethylen Barbara Barbosa, additional, Costa, Romero Marcos Pedrosa Brandão da, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2022
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16. BIONANOTECNOLOGIA: UMA NOVA TECNOLOGIA FRENTE ÀS ARBOVIROSES

Author

Rodrigues, Danielle Martiniano da Silva, primary, Araújo, Viviane Ferreira, additional, Santos, Micheline Thais dos, additional, Rolim, Tale Lucas Vieira, additional, Nascimento, Thiago Pajeú, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Batista, Juanize Matias da Silva, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2022
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17. APLICAÇÕES BIOTECNOLÓGICAS DO GÊNERO ABSIDIA

Author

Silva, Anna Carolina Batista e, primary, Nascimento, Maria Clara do, additional, Cardoso, Kethylen Barbara Barbosa, additional, Batista, Juanize Matias da Silva, additional, Oliveira, Vagne de Melo, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Porto, Ana Lucia Figueiredo, additional, and Nascimento, Thiago Pajeú, additional

Published
2022
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18. BIOTECNOLOGIA NA SAÚDE

Author

Oliveira, Vagne de Melo de, primary, Nascimento, Thiago Pajeú, additional, Batista, Juanize Matias da Silva, additional, Cardoso, Kethylen Barbara Barbosa, additional, Costa, Romero Marcos Pedrosa Brandão, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2022
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19. LACASES PRODUZIDAS POR MICRORGANISMOS: UMA REVISÃO

Author

Ferreira, Julyanne Victória dos Santos, primary, Neves, Anna Gabrielly Duarte, additional, Nascimento, Thiago Pajeú, additional, Oliveira, Vagne de Melo de, additional, Costa, Romero Marcos Pedrosa Brandão da, additional, Batista, Juanize Matias da Silva, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2022
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22. NANOTECNOLOGIA VERDE E SUAS APLICAÇÕES NO ECOSSISTEMA AGRÍCOLA

Author

Santos, Micheline Thais dos, primary, Rolim, Tale Lucas Vieira, additional, Araújo, Viviane Ferreira, additional, Barreiro, Maria Ercilia Lima, additional, Alves, Elizabeth Simões do Amaral, additional, Melo, Breno Araújo de, additional, Silva, Sybelle Georgia Mesquita da, additional, Brandão – Costa, Romero Marcos Pedrosa, additional, Batista, Juanize Matias da Silva, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2021
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24. Separation and partial purification of collagenolytic protease from peacock bass (Cichla ocellaris) using different protocol: Precipitation and partitioning approaches

Author

de Melo Oliveira, Vagne, Carneiro da Cunha, Márcia Nieves, Dias de Assis, Caio Rodrigo, Matias da Silva Batista, Juanize, Nascimento, Thiago Pajeú, dos Santos, Juliana Ferreira, de Albuquerque Lima, Carolina, de Araújo Viana Marques, Daniela, de Souza Bezerra, Ranilson, and Figueiredo Porto, Ana Lúcia

Published
2020
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26. Physicochemical Properties of a Bioactive Polysaccharide Film from Cassia grandis with Immobilized Collagenase from Streptomyces parvulus (DPUA/1573).

Author

Ferreira, Kétura, Cardoso, Kethylen, Brandão-Costa, Romero, Martins, Joana T., Botelho, Cláudia, Neves, Anna, Nascimento, Thiago, Batista, Juanize, Ferreira, Éverton, Damasceno, Fernando, Sales-Conniff, Amanda, Albuquerque, Wendell, Porto, Ana, and Teixeira, José

Subjects
POLYSACCHARIDES, CASSIA (Genus), STREPTOMYCES, COLLAGENASES, SKIN care
Abstract

(1) Background: Polysaccharide films are promising vehicles for the delivery of bioactive agents such as collagenases, as they provide controlled release at the wound site, facilitating tissue regeneration. This study aimed to investigate the physicochemical properties of Cassia grandis polysaccharide films with immobilized collagenase from Streptomyces parvulus (DPUA/1573). (2) Methods: Galactomannan was extracted from Cassia grandis seeds for film production with 0.8% (w/v) galactomannan and 0.2% (v/v) glycerol with or without collagenases. The films underwent physical-chemical analyses: Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), color and opacity (luminosity-L*, green to red-a*, yellow to blue-b*, opacity-Y%), moisture content, water vapor permeability (WVP), thickness, contact angle, and mechanical properties. (3) Results: The results showed similar FTIR spectra to the literature, indicating carbonyl functional groups. Immobilizing bioactive compounds increased surface roughness observed in SEM. TGA indicated a better viability for films with immobilized S. parvulus enzymes. Both collagenase-containing and control films exhibited a bright-yellowish color with slight opacity (Y%). Mechanical tests revealed decreased rigidity in PCF (−25%) and SCF (−41%) and increased deformability in films with the immobilized bioactive compounds, PCF (234%) and SCF (295%). (4) Conclusions: Polysaccharide-based films are promising biomaterials for controlled composition, biocompatibility, biodegradability, and wound healing, with a potential in pharmacological applications. [ABSTRACT FROM AUTHOR]

Published
2024
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27. BIOTECNOLOGIA MICROBIANA E SUAS APLICAÇÕES NA SAÚDE

Author

Costa, Romero Marcos Pedrosa Brandão, primary, Porto, Ana Lúcia Figueiredo, additional, Marques, Daniela de Araújo Viana, additional, Silva, Raphael Luiz Andrade, additional, Cardoso, Kethylen Barbara Barbosa, additional, Nascimento, Maria Clara do, additional, Silva, Maria Rafaele Oliveira Bezerra da, additional, Ferreira, Kétura Rhammá Cavalcante, additional, and Batista, Juanize Matias da Silva, additional

Published
2023
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28. BIOTECNOLOGIA NA SAÚDE - Volume III

Author

Costa, Romero Marcos Pedrosa Brandão, primary, Porto, Ana Lúcia Figueiredo, additional, Marques, Daniela de Araújo Viana, additional, Filho, José Pedro Martins Barbosa, additional, Batista, Juanize Matias da Silva, additional, Cardoso, Kethylen Barbara Barbosa, additional, Silva, Maria Rafaele Oliveira Bezerra da, additional, Nascimento, Maria Clara do, additional, and Nascimento, Thiago Pajeú, additional

Published
2023
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29. PARTIÇÃO DE PROTEASES FIBRINOLÍTICAS PRODUZIDAS POR ASPERGILLUS TAMARII KITA UCP 1279 ATRAVÉS DO SISTEMA DE DUAS FASES AQUOSAS PEGFOSFATO

Author

Alencar, Viviane do Nascimento e Silva, primary, Nascimento, Maria Clara do, additional, Ferreira, Julyanne Victória dos Santos, additional, Cunha, Márcia Nieves Carneiro da, additional, Batista, Juanize Matias da Silva, additional, Nascimento, Thiago Pajeú, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Porto, Ana Lucia Figueiredo, additional, and Leite, Ana Cristina Lima, additional

Published
2020
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30. PRODUÇÃO DE PROTEASES POR ASPERGILLUS TAMARII KITA UCP 1279 ISOLADO DA CAATINGA UTILIZANDO RESÍDUOS AGROINDUSTRIAIS

Author

Ferreira, Julyanne Victória dos Santos, primary, Cardoso, Kethylen Bárbara Barbosa, additional, Santos, Amanda Lucena dos, additional, Alencar, Viviane do Nascimento e Silva, additional, Nascimento, Maria Clara do, additional, Cunha, Marcia Nieves Carneiro da, additional, Batista, Juanize Matias da Silva, additional, Costa, Romero Pedrosa Brandão, additional, Nascimento, Thiago Pajeú, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2020
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34. LECTINAS DE FOLHAS DE Phthirusa pyrifolia

Author

BRANDÃO-COSTA, Romero Marcos Pedrosa, primary, BATISTA, Juanize Matias da Silva, additional, CARDOSO, Kethylen Barbara Barbosa, additional, ARAUJO, Vivianne Ferreira, additional, NASCIMENTO, Thiago Pajeú, additional, and PORTO, Ana Lucia Figueiredo, additional

Published
2020
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35. LECTINAS DE SEMENTES ENCONTRADAS NO BRAZIL: UMA REVISÃO SISTEMÁTICA

Author

CARDOSO, Kethylen Barbara Barbosa, primary, SANTOS, Sybelle Montenegro dos, additional, SANTOS, Amanda Lucena dos, additional, BATISTA, Juanize Matias da Silva, additional, NASCIMENTO, Thiago Pajeú, additional, BRANDÃO-COSTA, Romero Marcos Pedrosa, additional, and PORTO, Ana Lúcia Figueiredo, additional

Published
2020
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37. Extracellular collagenase isolated from Streptomyces antibioticus UFPEDA 3421: purification and biochemical characterization.

Author

Costa, Elizianne Pereira, Brandão-Costa, Romero Marcos Pedrosa, Albuquerque, Wendell Wagner Campos, Nascimento, Thiago Pajeú, Sales Conniff, Amanda Emmanuelle, Cardoso, Kethylen Barbara Barbosa, Neves, Anna Gabrielly Duarte, Batista, Juanize Matias da Silva, and Porto, Ana Lúcia Figueiredo

Subjects
COLLAGENASES, COFFEE grounds, STREPTOMYCES, PEPTIDES, ENZYMES
Abstract

Collagenases are proteases able to degrade native and denatured collagen, with broad applications such as leather, food, and pharmaceutical industries. The aim of this research was to purify and characterize a collagenase from Streptomyces antibioticus. In the present work, the coffee ground substrate provided conditions to obtaining high collagenase activity (377.5 U/mL) using anion-exchange DEAE-Sephadex G50 chromatographic protocol. SDS-PAGE revealed the metallo-collagenase with a single band of 41.28 kDa and was able to hydrolyzed type I and type V collagen producing bioactive peptides that delayed the coagulation time. The enzyme activity showed stability across a range of pH (6.0–11) and temperature (30–55 °C) with optima at pH 7.0 and 60 °C, respectively. Activators include Mg+2, Ca+2, Na+, K+, while full inhibition was given by other tested metalloproteinase inhibitors. Kinetic parameters (Km of 27.14 mg/mol, Vmax of 714.29 mg/mol/min, Kcat of 79.9 s−1 and Kcat/Km of 2.95 mL/mg/s) and thermodynamic parameters (Ea of 65.224 kJ/mol, ΔH of 62.75 kJ/mol, ΔS of 1.96 J/mol, ΔG of 62.16 kJ/mol, ΔGE-S of 8.18 kJ/mol and ΔGE-T of −2.64 kJ/mol) were also defined. Coffee grounds showed to be an interesting source to obtaining a collagenase able to produce bioactive peptides with anticoagulant activity. [ABSTRACT FROM AUTHOR]

Published
2024
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38. A novel β-fructofuranosidase produced by Penicillium citreonigrum URM 4459: purification and biochemical features

Author

Araújo, Vittoria Pradal Baracho, primary, Araújo, Thayza Kássia de, additional, Sousa, Karlla Mayara Nunes de, additional, Albuquerque, Wendell W. C., additional, Nascimento, Ana Karoline Caitano do, additional, Cardoso, Kethylen Barbara Barbosa, additional, Nascimento, Thiago Pajeú, additional, Batista, Juanize Matias da Silva, additional, Cavalcanti, Maria Taciana Holanda, additional, Porto, Ana Lucia Figueiredo, additional, and Costa, Romero Marcos Pedrosa Brandão, additional

Published
2022
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39. A novel β-fructofuranosidase produced by Penicillium citreonigrum URM 4459: purification and biochemical features.

Author

Araújo, Vittoria Pradal Baracho, Araújo, Thayza Kássia de, Sousa, Karlla Mayara Nunes de, Albuquerque, Wendell W. C., Nascimento, Ana Karoline Caitano do, Cardoso, Kethylen Barbara Barbosa, Nascimento, Thiago Pajeú, Batista, Juanize Matias da Silva, Cavalcanti, Maria Taciana Holanda, Porto, Ana Lucia Figueiredo, and Costa, Romero Marcos Pedrosa Brandão

Subjects
PENICILLIUM, INVERTASE, OCHRATOXINS, FRUCTOOLIGOSACCHARIDES, ISOELECTRIC point, MOLECULAR weights
Abstract

Fructooligosaccharides (FOS) are prebiotics of interest to the food industry. These compounds can be produced through the transfructosylation reaction by the enzyme fructofuranosidase. This enzyme is widely produced by fungi in a medium rich in sugar. Therefore, in this work, the main objectives were production, purification, biochemical characterization of a novel fructofuranosidase enzyme by Penicillium citreonigrum URM 4459 and synthesize and evaluate the antibacterial potential of fructooligosaccharides. With respect to sucrose hydrolysis, the optimal pH was 5.5, the apparent Km for purified FFase was 3.8 mM, the molecular mass was 43.0 kDa, estimated by gel filtration on Superdex increase G75 controlled by AKTA Avant 25 and confirmed by 10% SDS-PAGE under denaturing condition. Also, the isoelectric point was 4.9. The fractions obtained with enzymatic activities, both stable at acidic pH and high temperatures, as well as being able to produce FOS. Regarding antibacterial activity, the FOS produced in this study showed better results than commercial FOS and other carbon sources. Thus, this work presents relevant data for the use of P. citreonigum to produce fructofuranosidase and consequently FOS and can be used in the food and pharmaceutical industry. [ABSTRACT FROM AUTHOR]

Published
2023
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41. Proteasa con actividad fbrinolítica y colagenolítica producida por Aspergillus ochraceus URM604

Author

Cardoso, Kethylen Barbara Barbosa, Nascimento, Thiago Pajeú, Oliveira, Vagne de Melo, Costa, Romero Marcos Pedrosa Brandão, Pastrana, Lorenzo, Batista, Juanize Matias da Silva, and Porto, Ana Lucia Figueiredo

Subjects
Hongo Filamentoso, Fungo Filamentoso, Collagenase, Resíduo de Café, Salvado de Trigo, Filamentous Fungus, Fibrinolytic Enzyme, Colagenase, Coffee Residue, Colagenasa, Wheat Bran, Residuo de Café, Farelo de Trigo, Enzima Fibrinolítica
Abstract

Proteases play a fundamental role in the economy, being present in several industrial sectors. Among the fungi known to produce proteases, the genus Aspergillus stands out. Thus, the objective of this work was to produce proteases from solid state fermentation of Aspergillus ochraceus, purify, characterize and evaluate their potential in the production of collagenolytic and fibrinolytic enzymes. For this, the type and quantity of the substrate and the humidity of the fermentations were evaluated. Subsequently, purification was performed by DEA-Sephadex and Superdex-G75 chromatography in an FPLC system. As well as partial characterization evaluating the optimal pH and temperature of the protease action. It was observed that the best enzyme activity in fermentations with wheat bran as substrate (3g) and 60% moisture, showing protease, collagenolytic and fibrinolytic activity. Greater activity was also observed at pH8 and 50°C. Thus, it was concluded that A. ochraceus has great potential for biotechnological application, highlighting the importance of future evaluations. Las proteasas juegan un papel fundamental en la economía, estando presente en varios sectores industriales. Entre los hongos que se sabe que producen proteasas, se destaca el género Aspergillus. Así, el objetivo de este trabajo fue producir proteasas a partir de la fermentación en estado sólido de Aspergillus ochraceus, purificar, caracterizar y evaluar su potencial en la producción de enzimas colagenolíticas y fibrinolíticas. Para ello se evaluó el tipo y cantidad de sustrato y la humedad de las fermentaciones. Posteriormente, se realizó la purificación mediante cromatografía DEA-Sephadex y Superdex-G75 en sistema FPLC. Así como la caracterización parcial evaluando el pH y la temperatura óptimos de la acción de la proteasa. Se observó que la mejor actividad enzimática en fermentaciones con salvado de trigo como sustrato (3g) y 60% de humedad, presenta actividad proteasa, colagenolítica y fibrinolítica. También se observó una mayor actividad a pH 8 y 50 ° C. Así, se concluyó que A. ochraceus tiene un gran potencial de aplicación biotecnológica, destacando la importancia de futuras evaluaciones. Proteases possuem um papel fundamental na economia, estando presentes em diversos setores industriais. Dentre os fungos conhecidos por produzir proteases, destaca-se o gênero Aspergillus. Desta forma, o objetivo deste trabalho foi produzir proteases a partir de fermentação em estado sólido de Aspergillus ochraceus, purificar, caracterizar e avaliar seu potencial na produção de enzima colagenolítica e fibrinolítica. Para isto foram avaliados o tipo e quantidade do substrato e a umidade das fermentações. Posteriormente foi realizada purificação por cromatografia DEA-Sephadex e Superdex-G75 em sistema FPLC. Assim como caracterização parcial avaliando o pH e temperatura ótimos da ação da protease. Foi observada que a melhor atividade da enzima nas fermentações com farelo de trigo como substrato (3g) e 60% de umidade, apresentando atividade proteásica, colagenolítica e fibrinolítica. Também foi observada maior atividade em pH8 e 50°C. Assim, concluísse que A. ochraceus possui grande potencial para aplicação biotecnológica, destacando-se a importância de futuras avaliações.

Published
2022

44. Systematic analysis on the obtaining of fibrinolytic fungi enzymes

Author

Cardoso, Kethylen Barbara Barbosa, primary, Nascimento, Maria Clara, additional, Batista, Anna Carolina, additional, Oliveira, Vagne de Melo, additional, Nascimento, Thiago Pajeú, additional, Batista, Juanize Matias da Silva, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Pastrana, Lorenzo, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2022
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45. Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease

Author

Miranda, Vanessa Maria Andrade Machado, primary, Barbosa Filho, José Pedro Martins, additional, Costa, Romero Marcos Pedrosa Brandão, additional, Leite, Ana Cristina Lima, additional, Oliveira, Vagne de Melo, additional, Batista, Juanize Matias da Silva, additional, Pastrana, Lorenzo, additional, Nascimento, Thiago Pajeú, additional, and Porto, Ana Lúcia Figueiredo, additional

Published
2022
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46. A novel collagenolytic protease from Mucor subtilissimus UCP 1262: Comparative analysis of production and extraction in submerged and stated-solid fermentation

Author

SOUZA, KESSIA P.S., primary, CUNHA, MÁRCIA N.C., additional, BATISTA, JUANIZE M.S., additional, OLIVEIRA, VAGNE M., additional, NASCIMENTO, THIAGO P., additional, CONNIFF, AMANDA E.S., additional, COSTA, ROMERO M.P.B., additional, PORTO, TATIANA S., additional, PORTO, CAMILA S., additional, and PORTO, ANA LÚCIA F., additional

Published
2022
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47. Purification and characterization of fibrinolytic protease from Streptomyces parvulus by polyethylene glycol-phosphate aqueous two-phase system

Author

ALENCAR,VIVIANE N.S., NASCIMENTO,MARIA CLARA DO, FERREIRA,JULYANNE V. DOS SANTOS, BATISTA,JUANIZE M. DA SILVA, CUNHA,MARCIA N.C. DA, NASCIMENTO,JÉSSICA M. DO, SOBRAL,RENATA V. DA SILVA, COUTO,MILENA T.T. DO, NASCIMENTO,THIAGO P., COSTA,ROMERO M.P.B., PORTO,ANA LÚCIA F., and LEITE,ANA CRISTINA L.

Subjects
thrombolytic, Actinomycetes, protease, fibrinolysis
Abstract

Fibrinolytic proteases are a promising alternative in the pharmaceutical industry, they are used in the treatment of cardiovascular diseases, especially thrombosis. Microorganisms are the most interesting source of fibrinolytic proteases. The aim of this study was the production of fibrinolytic protease from Streptomyces parvulus DPUA 1573, the recovery of the protease by aqueous two-phase system and partial biochemical characterization of the enzyme. The aqueous two-phase system was performed according to a 24-full factorial design using polyethylene glycol molar mass, polyethylene glycol concentration, citrate concentration and pH as independent variables. It was analyzed the effect of different ions, surfactants, inhibitors, pH and temperature on enzyme activity. The best conditions for purifying the enzyme were 17.5% polyethylene glycol 8,000, 15% Phosphate and pH 8.0, it was obtained a partition coefficient of 7.33, a yield of 57.49% and a purification factor of 2.10-fold. There was an increase in enzyme activity in the presence of Fe2+ and a decrease in the presence of $\beta$-Mercaptoethanol, phenylmethylsulfonyl fluoride and Iodoacetic acid. The optimum pH was 7.0 and the optimum temperature was 40 ºC. The purified protease exhibited a molecular mass of 41 kDa. The fibrinolytic protease from Streptomyces parvulus proved to be a viable option for the development of a possible drug with fibrinolytic action.

Published
2021

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