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5 results on '"Bavaro, S.L."'

1. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

Author

Lozano-Ojalvo, D., Benede, S., Antunes, C.M., Bavaro, S.L., Bouchaud, G., Costa, A., Denery-Papini, S., Diaz-Perales, A., Garrido-Arandia, M., Gavrovic-Jankulovic, M., Hayen, S., Martinez-Blanco, M., Molina, E., Monaci, L., Pieters, R.H.H., Villemin, C., Wichers, H.J., Wroblewska, B., Willemsen, L.E.M., Roggen, E.L., Bilsen, J.H.M. van, Lozano-Ojalvo, D., Benede, S., Antunes, C.M., Bavaro, S.L., Bouchaud, G., Costa, A., Denery-Papini, S., Diaz-Perales, A., Garrido-Arandia, M., Gavrovic-Jankulovic, M., Hayen, S., Martinez-Blanco, M., Molina, E., Monaci, L., Pieters, R.H.H., Villemin, C., Wichers, H.J., Wroblewska, B., Willemsen, L.E.M., Roggen, E.L., and Bilsen, J.H.M. van

Abstract

Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches. A© 2019 Elsevier Ltd

Published
2019

2. Application of the adverse outcome pathway (AOP) concept to structure the available in vivo and in vitro mechanistic data for allergic sensitization to food proteins

Author

Bilsen, J.H.M. van, Sienkiewicz-Szłapka, E., Lozano-Ojalvo, D., Willemsen, L.E.M., Antunes, C.M., Molina, E., Smit, J.J., Wróblewska, B., Wichers, H.J., Knol, E.F., Ladics, G.S., Pieters, R.H.H., Denery-Papini, S., Vissers, Y.M., Bavaro, S.L., Larré, C., Verhoeckx, K.C.M., and Roggen, E.L.

Subjects
RAPID - Risk Analysis for Products in Development, Biomedical Innovation, Key event relations, Molecular initiating event, Sensitization, Food proteins, Adverse outcome pathway, Life, Food allergy, Mechanistic understanding, ELSS - Earth, Life and Social Sciences, Biology, Healthy Living, Key events
Abstract

Background: The introduction of whole new foods in a population may lead to sensitization and food allergy. This constitutes a potential public health problem and a challenge to risk assessors and managers as the existing understanding of the pathophysiological processes and the currently available biological tools for prediction of the risk for food allergy development and the severity of the reaction are not sufficient. There is a substantial body of in vivo and in vitro data describing molecular and cellular events potentially involved in food sensitization. However, these events have not been organized in a sequence of related events that is plausible to result in sensitization, and useful to challenge current hypotheses. The aim of this manuscript was to collect and structure the current mechanistic understanding of sensitization induction to food proteins by applying the concept of adverse outcome pathway (AOP). Main body: The proposed AOP for food sensitization is based on information on molecular and cellular mechanisms and pathways evidenced to be involved in sensitization by food and food proteins and uses the AOPs for chemical skin sensitization and respiratory sensitization induction as templates. Available mechanistic data on protein respiratory sensitization were included to fill out gaps in the understanding of how proteins may affect cells, cell-cell interactions and tissue homeostasis. Analysis revealed several key events (KE) and biomarkers that may have potential use in testing and assessment of proteins for their sensitizing potential. Conclusion: The application of the AOP concept to structure mechanistic in vivo and in vitro knowledge has made it possible to identify a number of methods, each addressing a specific KE, that provide information about the food allergenic potential of new proteins. When applied in the context of an integrated strategy these methods may reduce, if not replace, current animal testing approaches. The proposed AOP will be shared at the www.aopwiki.org platform to expand the mechanistic data, improve the confidence in each of the proposed KE and key event relations (KERs), and allow for the identification of new, or refinement of established KE and KERs. © 2017 The Author(s).

Published
2017

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