11 results on '"Bearzotti, Mariela"'
Search Results
2. A test field for the second-order advantage in bilinear least-squares and parallel factor analyses: fluorescence determination of ciprofloxacin in human urine
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Damiani, Patricia C., Nepote, Alberto J., Bearzotti, Mariela, and Olivieri, Alejandro C.
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Chemistry, Analytic -- Research ,Chemistry - Abstract
The analytical performances of two algorithms, the recently introduced bilinear least-squares (BLLS) and the popular parallel factor analysis (PARAFAC), are compared as regards second-order fluorescence data recorded for the determination of the fluoroquinolone antibiotic ciprofloxacin in human urine samples. The applied chemometric methodologies employ different strategies for exploiting the so-called second-order advantage, which allows one to obtain individual concentrations of calibrated analytes in the presence of any number of uncalibrated (urine) components. Analysis of a spiked urine test set (in the analyte concentration range 0-200 mg [L.sup.-1]) showed that BLLS provides results of slightly better quality than PARAFAC. Satisfactory results have been obtained on comparing the concentrations predicted for a series of real urine samples with those furnished by liquid chromatography. The limit of detection of the fluorescence-based methods is ~5 mg [L.sup.-1].
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- 2004
3. Spectrofluorometric determination of naproxen in tablets
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Damiani, Patricia, Bearzotti, Mariela, and Cabezón, Miguel A
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- 2002
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4. Preferential association of a functional variant in complement receptor 2 with antibodies to double-stranded DNA
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Zhao, Jian, Giles, Brendan M., Taylor, Rhonda L., Yette, Gabriel A., Lough, Kara M., Han Leng, Ng, Abraham, Lawrence J., Hui, Wu, Kelly, Jennifer A., Glenn, Stuart B., Adler, Adam J., Williams, Adrienne H., Comeau, Mary E., Ziegler, Julie T., Marion, Miranda, Alarcón Riquelme, Marta E., Alarcón, Graciela S., Anaya, Juan Manuel, Bae, Sang Cheol, Kim, Dam, Lee, Hye Soon, Criswell, Lindsey A., Freedman, Barry I., Gilkeson, Gary S., Guthridge, Joel M., Jacob, Chaim O., James, Judith A., Kamen, Diane L., Merrill, Joan T., Sivils, Kathy Moser, Niewold, Timothy B., Petri, Michelle A., Ramsey Goldman, Rosalind, Reveille, John D., Scofield, R. Hal, Stevens, Anne M., Vilá, Luis M., Vyse, Timothy J., Kaufman, Kenneth M., Harley, John B., Langefeld, Carl D., Gaffney, Patrick M., Brown, Elizabeth E., Edberg, Jeffrey C., Kimberly, Robert P., Ulgiati, Daniela, Tsao, Betty P., Boackle, Susan A., Frostegård, Johan, Truedsson, Lennart, De Ramón, Enrique, Sabio, José M., González Escribano, María F., Martin, Javier, Ortego Centeno, Norberto, Callejas, José Luis, Sánchez Román, Julio, D'Alfonso, Sandra, Migliarese, Sergio, Sebastiani, Gian Domenico, Galeazzi, Mauro, Witte, Torsten, Lauwerys, Bernard R., Endreffy, Emoke, Kovács, László, Vasconcelos, Carlos, Da Silva, Berta Martins, Scherbarth, R., Marino, Pilar C., Motta, Estela L., Gamron, Susana, Drenkard, Cristina, Menso, Emilia, Allievi, Alberto, Tate, Guillermo A., Presas, Jose L., Palatnik, Simon A., Abdala, Marcelo, Bearzotti, Mariela, Alvarellos, Alejandro, Caeiro, Francisco, Bertoli, Ana, Paira, Sergio, Roverano, Susana, Graf, Cesar E., Bertero, Estela, Caprarulo, Cesar, Buchanan, Griselda, Guillerón, Carolina, Grimaudo, Sebastian, Manni, Jorge, Catoggio, Luis J., Soriano, Enrique R., Santos, Carlos D., Prigione, Cristina, Ramos, Fernando A., Navarro, Sandra M., Berbotto, Guillermo A., Jorfen, Marisa, Romero, Elisa J., Garcia, Mercedes A., Marcos, Juan C., Marcos, Ana I., Perandones, Carlos E., Eimon, Alicia, Parque, Sanatorio, Battagliotti, Cristina G., Acevedo, Eduardo, Cucho, Mariano, De La Torre, Ignacio García, Ríos, Mario Cardiel, Moctezuma, José Francisco, and Ceceña, Marco Maradiaga
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Genetics and Molecular Biology (all) ,CR1 protein, human ,Anti-nuclear antibody ,Intron ,Pathogenesis ,Complement receptor ,Real time polymerase chain reaction ,Procedures ,Biochemistry ,Haplotype ,Basic and Translational Research ,Systemic lupus erythematosus ,Messenger RNA ,3. Good health ,Blood ,Human ,Genotype ,Immunology ,Case control study ,B-Lymphocyte Subsets ,Single-nucleotide polymorphism ,Major clinical study ,Biosynthesis ,Risk Assessment ,Article ,General Biochemistry, Genetics and Molecular Biology ,Double stranded DNA antibody ,03 medical and health sciences ,Gene Polymorphism ,Rheumatology ,Genetics ,Humans ,Polymorphism ,Autoantibodies ,B cells ,Genetic predisposition ,Systemic ,Genetic Variation ,DNA ,medicine.disease ,030104 developmental biology ,Case-Control Studies ,Receptors, Complement 3b ,Receptors, Complement 3d ,Complement component C3b receptor ,Transcription factor ,Transcription Factors ,0301 basic medicine ,Unclassified drug ,Complement receptor 2 ,Systemic Lupus Erythematosus ,Adolescent ,Adult ,Antibodies, Antinuclear ,Genetic Predisposition to Disease ,Haplotypes ,Lupus Erythematosus, Systemic ,Middle Aged ,Phenotype ,Polymorphism, Single Nucleotide ,Young Adult ,Immunology and Allergy ,Medicine (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Complement receptor 1 ,Antinuclear ,Receptors ,Flow cytometry ,Middle aged ,Priority journal ,Risk assessment ,Allele ,biology ,Single Nucleotide ,Complement component C3d receptor ,Chromatin immunoprecipitation ,Transcription factor CTCF ,Antibodies ,DNA protein complex ,Antinuclear antibody ,medicine ,Genetic variation ,Lupus erythematosus ,B lymphocyte ,Lupus Erythematosus ,Complement 3d ,Complement 3b ,Molecular biology ,Single nucleotide polymorphism ,Minor allele frequency ,Metabolism ,Genetic association ,Genetic variability ,Gel mobility shift assay ,biology.gene ,Controlled study - Abstract
Objectives Systemic lupus erythematosus (SLE; OMIM 152700) is characterised by the production of antibodies to nuclear antigens. We previously identified variants in complement receptor 2 (CR2/CD21) that were associated with decreased risk of SLE. This study aimed to identify the causal variant for this association. Methods Genotyped and imputed genetic variants spanning CR2 were assessed for association with SLE in 15 750 case-control subjects from four ancestral groups. Allele-specific functional effects of associated variants were determined using quantitative real-time PCR, quantitative flow cytometry, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP)-PCR. Results The strongest association signal was detected at rs1876453 in intron 1 of CR2 (pmeta=4.2×10-4, OR 0.85), specifically when subjects were stratified based on the presence of dsDNA autoantibodies (case-control pmeta=7.6×10-7, OR 0.71; case-only pmeta=1.9×10-4, OR 0.75). Although allele-specific effects on B cell CR2 mRNA or protein levels were not identified, levels of complement receptor 1 (CR1/CD35) mRNA and protein were significantly higher on B cells of subjects harbouring the minor allele (p=0.0248 and p=0.0006, respectively). The minor allele altered the formation of several DNA protein complexes by EMSA, including one containing CCCTC-binding factor (CTCF), an effect that was confirmed by ChIP-PCR. Conclusions These data suggest that rs1876453 in CR2 has long-range effects on gene regulation that decrease susceptibility to lupus. Since the minor allele at rs1876453 is preferentially associated with reduced risk of the highly specific dsDNA autoantibodies that are present in preclinical, active and severe lupus, understanding its mechanisms will have important therapeutic implications.
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- 2016
5. Antiphospholipid and antioangiogenic activity in females with recurrent miscarriage and antiphospholipid syndrome
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Pelusa, Hector F, primary, Pezzarini, Eleonora, additional, Basiglio, Cecilia L, additional, Musuruana, Jorge, additional, Bearzotti, Mariela, additional, Svetaz, María J, additional, Daniele, Stella M, additional, Bottai, Hebe, additional, and Arriaga, Sandra MM, additional
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- 2016
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6. Preferential Binding to Elk-1 by SLE-Associated IL10 Risk Allele Upregulates IL10 Expression
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Sakurai, Daisuke, Zhao, Jian, Deng, Yun, Kelly, Jennifer A., Brown, Elizabeth E., Harley, John B., Bae, Sang Cheol, Alarcn-Riquelme, Marta E., Edberg, Jeffrey C., Kimberly, Robert P., Ramsey-Goldman, Rosalind, Petri, Michelle A., Reveille, John D., Vilá, Luis M., Alarcón, Graciela S., Kaufman, Kenneth M., Vyse, Timothy J., Jacob, Chaim O., Gaffney, Patrick M., Sivils, Kathy Moser, James, Judith A., Kamen, Diane L., Gilkeson, Gary S., Niewold, Timothy B., Merrill, Joan T., Scofield, R. Hal, Criswell, Lindsey A., Stevens, Anne M., Boackle, Susan A., Kim, Jae Hoon, Choi, Jiyoung, Pons-Estel, Bernardo A., Freedman, Barry I., Anaya, Juan Manuel, Martin, Javier, Yu, C. Yung, Chang, Deh Ming, Song, Yeong Wook, Langefeld, Carl D., Chen, Weiling, Grossman, Jennifer M., Cantor, Rita M., Hahn, Bevra H., Tsao, Betty P., Frostegård, Johan, Truedsson, Lennart, de Ramón, Enrique, Sabio, José M., Ortego-Centeno, Norberto, CAllejas, José Luis, González-Escribano, María F., Sánchez-Román, Julio, D'Alfonso, Sandra, Migliarese, Sergio, Sebastiani, Gian Domenico, Galeazzi, Mauro, Witte, Torsten, Lauwerys, Bernard R., Endreffy, Emoke, Kovács, László, Vasconcelos, Carlos, da Silva, Berta Martins, Scherbarth, Hugo R., Marino, Pilar C., Motta, Estela L., Gamron, Susana, Drenkard, Cristina, Menso, Emilia, Allievi, Alberto, Tate, Guillermo A., Presas, Jose L., Palatnik, Simon A., Abdala, Marcelo, Bearzotti, Mariela, Alvarellos, Alejandro, Caeiro, Francisco, Bertoli, Ana, Paira, Sergio, Roverano, Susana, Graf, Cesar E., Bertero, Estela, Caprarulo, Cesar, Buchanan, Griselda, Guillerón, Carolina, Grimaudo, Sebastian, Manni, Jorge, Catoggio, Luis J., Soriano, Enrique R., Santos, Carlos D., Prigione, Cristina, Ramos, Fernando A., Navarro, Sandra M., Berbotto, Guillermo A., Jorfen, Marisa, Romero, Elisa J., Garcia, Mercedes A., Marcos, Juan C., Marcos, Ana I., Perandones, Carlos E., Eimon, Alicia, Parque, Sanatorio, Battagliotti, Cristina G., Acevedo, Eduardo, Cucho, Mariano, de la Torre, Ignacio García, Ríos, Mario Cardiel, Moctezuma, José Francisco, and Ceceña, Marco Maradiaga
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Cancer Research ,medicine.medical_treatment ,Genome-wide association study ,Gene ,Disease predisposition ,0302 clinical medicine ,Interleukin 10 ,immune system diseases ,Lupus Erythematosus, Systemic ,Disease activity ,skin and connective tissue diseases ,Genetics (clinical) ,Regulation of gene expression ,0303 health sciences ,TGranscription factor Elk 1 ,Hispanic or Latino ,Interleukin-10 ,Up-Regulation ,3. Good health ,Cytokine ,Research Article ,Protein Binding ,Genotype ,lcsh:QH426-470 ,Inmunología ,Single-nucleotide polymorphism ,Down regulation ,Biology ,Polymorphism, Single Nucleotide ,White People ,03 medical and health sciences ,Asian People ,Enfermedades autoinmunes ,Lupus eritematoso sistémico ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Electrophoretic mobility shift assay ,Gene cluster ,Allele ,Molecular Biology ,Alleles ,Ecology, Evolution, Behavior and Systematics ,ets-Domain Protein Elk-1 ,030304 developmental biology ,Lupus erythematosus ,medicine.disease ,Enfermedades ,Introns ,lcsh:Genetics ,Binding affinity ,Gene Expression Regulation ,Haplotypes ,Immunology ,Controlled study ,Genome-Wide Association Study ,030215 immunology - Abstract
Immunoregulatory cytokine interleukin-10 (IL-10) is elevated in sera from patients with systemic lupus erythematosus (SLE) correlating with disease activity. The established association of IL10 with SLE and other autoimmune diseases led us to fine map causal variant(s) and to explore underlying mechanisms. We assessed 19 tag SNPs, covering the IL10 gene cluster including IL19, IL20 and IL24, for association with SLE in 15,533 case and control subjects from four ancestries. The previously reported IL10 variant, rs3024505 located at 1 kb downstream of IL10, exhibited the strongest association signal and was confirmed for association with SLE in European American (EA) (P = 2.7×10−8, OR = 1.30), but not in non-EA ancestries. SNP imputation conducted in EA dataset identified three additional SLE-associated SNPs tagged by rs3024505 (rs3122605, rs3024493 and rs3024495 located at 9.2 kb upstream, intron 3 and 4 of IL10, respectively), and SLE-risk alleles of these SNPs were dose-dependently associated with elevated levels of IL10 mRNA in PBMCs and circulating IL-10 protein in SLE patients and controls. Using nuclear extracts of peripheral blood cells from SLE patients for electrophoretic mobility shift assays, we identified specific binding of transcription factor Elk-1 to oligodeoxynucleotides containing the risk (G) allele of rs3122605, suggesting rs3122605 as the most likely causal variant regulating IL10 expression. Elk-1 is known to be activated by phosphorylation and nuclear localization to induce transcription. Of interest, phosphorylated Elk-1 (p-Elk-1) detected only in nuclear extracts of SLE PBMCs appeared to increase with disease activity. Co-expression levels of p-Elk-1 and IL-10 were elevated in SLE T, B cells and monocytes, associated with increased disease activity in SLE B cells, and were best downregulated by ERK inhibitor. Taken together, our data suggest that preferential binding of activated Elk-1 to the IL10 rs3122605-G allele upregulates IL10 expression and confers increased risk for SLE in European Americans., Author Summary Systemic lupus erythematosus (SLE), a debilitating autoimmune disease characterized by the production of pathogenic autoantibodies, has a strong genetic basis. Variants of the IL10 gene, which encodes cytokine interleukin-10 (IL-10) with known function of promoting B cell hyperactivity and autoantibody production, are associated with SLE and other autoimmune diseases, and serum IL-10 levels are elevated in SLE patients correlating with increased disease activity. In this study, to discover SLE-predisposing causal variant(s), we assessed variants within the genomic region containing IL10 and its gene family member IL19, IL20 and IL24 for association with SLE in case and control subjects from diverse ancestries. We identified SLE-associated SNP rs3122605 located at 9.2 kb upstream of IL10 as the most likely causal variant in subjects of European ancestry. The SLE-risk allele of rs3122605 was dose-dependently associated with elevated IL10 expression at both mRNA and protein levels in peripheral blood samples from SLE patients and controls, which could be explained, at least in part, by its preferential binding to Elk-1, a transcription factor activated in B cells during active disease of SLE patients. Elk-1-mediated IL-10 overexpression could be downregulated by inhibiting activation of mitogen-activated protein kinases, suggesting a potential therapeutic target for SLE.
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- 2013
7. Identification of a new putative functional IL18 gene variant through an association study in systemic lupus erythematosus
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Sánchez, Elena, Palomino Morales, Rogelio J., Ortego Centeno, Norberto, Jiménez Alonso, Juan, González Gay, Miguel A., López Nevot, Miguel A., Sánchez Román, Julio, de Ramón, Enrique, González Escribano, M. Francisca, Pons Estel, Bernardo A., D'Alfonso, Sandra, Sebastiani, Gian Domenico, Alarcón Riquelme, Marta E., Martín, Javier, Scherbarth, Hugo R., Marino, Pilar C., Motta, Estela L., Gamron, Susana, Drenkard, Cristina, Menso, Emilia, Allievi, Alberto, Tate, Guillermo A., Presas, Jose L., Palatnik, Simon A., Abdala, Marcelo, Bearzotti, Mariela, Alvarellos, Alejandro, Caeiro, Francisco, Bertoli, Ana, Paira, Sergio, Roverano, Susana, Graf, Cesar E., Bertero, Estela, Caprarulo, Cesar, Buchanan, Griselda, Guillerón, Carolina, Grimaudo, Sebastian, Manni, Jorge, Catoggio, Luis J., Soriano, Enrique R., Santos, Carlos D., Prigione, Cristina, Ramos, Fernando A., Navarro, Sandra M., Berbotto, Guillermo A., Jorfen, Marisa, Romero, Elisa J., Garcia, Mercedes A., Marcos, Juan C, Marcos, Ana I., Perandones, Carlos E., Eimon, Alicia, Battagliotti, Cristina G., Barizzone, Nadia, Galeazzi, Mauro, Danieli, Maria Giovanna, Migliaresi, Sergio, and Bozzolo, Enrica
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Genotype ,European Continental Ancestry Group ,Gene Expression ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,White People ,Chromosomes ,Cell Line ,TaqMan ,Genetics ,Lupus Erythematosus, Systemic ,SNP ,Humans ,Genetic Predisposition to Disease ,Allele ,Pair 11 ,Polymorphism ,Gene ,Molecular Biology ,Genetics (clinical) ,Genetic association ,Case-Control Studies ,Chromosome Mapping ,Chromosomes, Human, Pair 11 ,Genetic Variation ,Interleukin-18 ,Genome-Wide Association Study ,Lupus Erythematosus ,Systemic ,General Medicine ,Single Nucleotide ,Immunology ,Interleukin 18 ,Human - Abstract
Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role in chronic inflammation and autoimmune disorders. In this study, we aimed to determine the potential role of the IL18 gene in SLE. To define the genetic association of the IL18 and SLE, we have genotyped nine SNPs in an independent set of Spanish cases and controls. The IL18 polymorphisms were genotyped by PCR, using a predeveloped TaqMan allele discrimination assay. Two SNPs were still significant after fine mapping of the IL18 gene. The SNP (rs360719) surviving correction for multiple tests was genotyped in two replication cohorts from Italy and Argentina. After the analysis, a significance with rs360719 C-allele remained across the sets and after the meta-analysis (Pooled OR = 1.37, 95% CI 1.21-1.54, combined P = 3.8E-07, Pc = 1.16E-06). Quantitative real-time PCR was performed to assess IL18 mRNA expression in PBMC from subjects with different IL18 rs360719 genotypes. We tested the effect of the IL18 rs360719 polymorphism on the transcription of IL18 by electrophoretic mobility shift assay and western blot. We found a significant increase in the relative expression of IL18 mRNA in individuals carrying the rs360719 C-risk allele; in addition we show that the polymorphism creates a binding site for the transcriptional factor OCT-1. These findings suggest that the novel IL18 rs360719 variant may play an important role in determining the susceptibility to SLE and it could be a key factor in the expression of the IL18 gene.
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- 2009
8. Antiphospholipid and antioangiogenic activity in females with recurrent miscarriage and antiphospholipid syndrome.
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Pelusa, Hector F., Pezzarini, Eleonora, Basiglio, Cecilia L., Musuruana, Jorge, Bearzotti, Mariela, Svetaz, María J., Daniele, Stella M., Bottai, Hebe, Arriaga, Sandra M. M., and Arriaga, Sandra Mm
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ANTIPHOSPHOLIPID syndrome ,PHOSPHOLIPID antibodies ,RECURRENT miscarriage ,ANNEXINS ,BIOMARKERS ,PREGNANCY complications ,VASCULAR endothelial growth factor receptors ,AUTOANTIBODIES ,CALCIUM-binding proteins ,CELL receptors ,GLYCOPROTEINS ,IMMUNOLOGICAL adjuvants ,NEOVASCULARIZATION ,RETROSPECTIVE studies ,BLOOD ,DISEASE complications - Abstract
Background Antiphospholipid syndrome is an autoimmune disease characterized by thrombosis, fetal losses and thrombocytopenia associated to antiphospholipid antibodies. They are directed to phospholipids, such as cardiolipins (anticardiolipin) and lupus anticoagulant or to complexes formed by phospholipids and protein cofactors, such as β2 glycoprotein 1 (a-β2GP1) and annexin V (a-annexin V). These auto-antibodies may be considered as a family of antibodies involved in thrombotic events and antiphospholipid activity. On the other hand, some proangiogenic factors are involved in the normal development of placental vasculature, such as the vascular endothelial growth factor. Overexpression of vascular endothelial growth factor receptor in its soluble form (sVEGFR-1) has been associated to a higher antiangiogenic activity. Our aim was to analyse the association between anticardiolipin, lupus anticoagulant, a-β2GP1, a-annexin V and sVEGFR-1 with recurrent miscarriage before week 10 of gestation in females with antiphospholipid syndrome. Methods We studied 24 females (primary or secondary antiphospholipid syndrome), who were divided into two groups: females with recurrent miscarriage before week 10 of gestation (M; n = 12) and females with no history of fetal loss (NM; n = 12). Anticardiolipin, a-β2GP1, a-annexin V and sVEGF-R1 concentrations were assessed by ELISA, while lupus anticoagulant was assessed by screening and confirmatory tests. Results A significant association was observed between the number of positive biomarkers and the belonging group ( P < 0.05). Besides, a positive result for lupus anticoagulant and a-β2GP1 was found to be significantly associated to the M group ( P < 0.05). Conclusions Lupus anticoagulant and a-β2GP1 may be implicated in pregnancies complicated by recurrent miscarriage in females with antiphospholipid syndrome. [ABSTRACT FROM AUTHOR]
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- 2017
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9. Quantification of autoantibodies to annexin V in plasma by an "in house" sandwich ELISA
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Pelusa, Héctor F., primary, Valdés, María de los Ángeles, additional, Daniele, Stella M., additional, Almará, Adriana M., additional, Svetaz, María J., additional, Bearzotti, Mariela, additional, Paparella, Cecilia V., additional, Bouvet, Beatriz R., additional, Ghersevich, Sergio A., additional, and Arriaga, Sandra M., additional
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- 2012
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10. Spectrofluorometric determination of ibuprofen in pharmaceutical formulations
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Damiani, Patricia C, primary, Bearzotti, Mariela, additional, and Cabezón, Miguel A, additional
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- 2001
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11. Spectrofluorometric determination of diclofenac in tablets and ointments
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Damiani, Patricia C, primary, Bearzotti, Mariela, additional, Cabezón, Miguel A, additional, and Olivieri, Alejandro C, additional
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- 1999
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