15 results on '"Bedoya, Katherine"'
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2. Annual microbial community dynamics in a full-scale anaerobic sludge digester from a wastewater treatment plant in Colombia
- Author
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Bedoya, Katherine, Hoyos, Oladier, Zurek, Eduardo, Cabarcas, Felipe, and Alzate, Juan F.
- Published
- 2020
- Full Text
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3. Influence of different stress factors during the elaboration of grape must’s pied-de-cuve on the dynamics of yeast populations during alcoholic fermentation
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Bedoya, Katherine, primary, Buetas, Luis, additional, Rozès, Nicolas, additional, Mas, Albert, additional, and Portillo, M. Carmen, additional
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- 2024
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- View/download PDF
4. Evaluation of the chemical and organoleptic profiles of wines inoculated with different pied de cuve
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Ministerio de Ciencia, Innovación y Universidades, Universidad Rovira y Virgili (URV), Universidad de Chile, Bedoya, Katherine, Rozès, Nicolas, Mas, Albert, Jara, Carla, Bordons, Albert, Portillo, M. Carmen, Ministerio de Ciencia, Innovación y Universidades, Universidad Rovira y Virgili (URV), Universidad de Chile, Bedoya, Katherine, Rozès, Nicolas, Mas, Albert, Jara, Carla, Bordons, Albert, and Portillo, M. Carmen
- Abstract
The technique known as pied de cuve (PdC) serves as an inoculation starter and improves the microbial footprint of each wine region. The PdC methodology, which translates as "bottom of the deposit" in French, serves as a method to initiate alcoholic fermentation (AF). PdC involves using a small volume of already fermenting must to start the AF when added to fresh must. There is a lack of information about the chemical and sensory impact of the final wines inoculated with PdC. In this study, we compared the sensory and chemical impact of the PdC technique in three different harvests using Muscat of Alexandria Must (Spain, harvests 2022 and 2023) and Sauvignon Blanc (Chile, harvest 2023). Each PdC was prepared using different stressors (sulphur dioxide, ethanol, and temperature). Our results showed that wines elaborated with PdC had similar fermentation kinetics and resulted in a lower yield of acetic acid compared to both the inoculation of commercial strains and wine produced by spontaneous fermentation. Additionally, wines made by PdC technique had similar global quality scores in the sensory profiles to wines produced by inoculation of commercial strains for both harvests from 2023. However, wines elaborated with PdC showed an additional significant tropical attribute in the case of Spanish 2022 and Chilean 2023 harvests when compared to wine produced by the inoculation of a commercial yeast.
- Published
- 2024
5. The Impact of the Inoculation of Different Pied de Cuve on the Chemical and Organoleptic Profiles of Wines.
- Author
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Bedoya, Katherine, Mas, Albert, Rozès, Nicolas, Jara, Carla, and Portillo, María del Carmen
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FERMENTATION ,WINE districts ,SACCHAROMYCES cerevisiae ,SULFUR dioxide ,WINES - Abstract
Controlling the microorganisms involved in alcoholic fermentation during wine production can be achieved by adding a small quantity of spontaneously fermenting must to freshly crushed grapes, a technique known as pied de cuve (PdC). This method not only serves as an inoculation starter but also enhances the microbial footprint unique to each wine region. Recent studies have confirmed that wines inoculated with PdC exhibit efficient fermentation kinetics comparable to those inoculated with commercial strains of Saccharomyces cerevisiae. However, further research is required to draw robust conclusions about the chemical and sensory impacts of PdC-inoculated wines. In this study, we examined the chemical and sensory effects of the PdC technique across three different harvests: Muscat of Alexandria (Spain, harvests 2022 and 2023) and Sauvignon Blanc (Chile, harvest 2023). Each PdC was prepared using various stressors (sulfur dioxide, ethanol, and temperature). Our findings revealed that wines produced with PdC exhibited similar fermentation kinetics and sensory profiles to those inoculated with commercial strains. Notably, PdC fermentations resulted in lower concentrations of acetic acid compared to both the commercial strain and spontaneous fermentations. The sensory analysis indicated that PdC wines significantly differed from those made with commercial strains, with PdC wines displaying more pronounced tropical notes. These results suggest that the PdC technique, particularly when using specific stressors, can maintain desirable fermentation characteristics while enhancing certain sensory attributes, offering a viable alternative to traditional inoculation methods. [ABSTRACT FROM AUTHOR]
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- 2024
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- View/download PDF
6. Metagenomic assessment of the microbial community and methanogenic pathways in biosolids from a municipal wastewater treatment plant in Medellín, Colombia
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Bedoya, Katherine, Coltell, Oscar, Cabarcas, Felipe, and Alzate, Juan F.
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- 2019
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7. Responsabilidad social y marketing educativo: herramientas competitivas para las organizaciones universitarias
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Rubio-Rodríguez, Gustavo Adolfo, primary, Hernández, Heber Alfredo Guifo, additional, Cerquera, Eva María Villamil, additional, and Bedoya, Katherine Espinosa, additional
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- 2022
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8. Metagenomic Analysis of Biocide-Treated Neotropical Oil Reservoir Water Unveils Microdiversity of Thermophile Tepidiphilus
- Author
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Bedoya, Katherine, primary, Niño, Jhorman, additional, Acero, Julia, additional, Jaimes-Prada, Ronald, additional, Cabarcas, Felipe, additional, and Alzate, Juan F., additional
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- 2021
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9. Bacterial diversity analysis of freshwater sources for human use in rural areas of the tropical Andean region of Colombia
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Bedoya, Katherine, primary, Galeano, Laila, additional, Hincapié, Margarita M., additional, Mesa, Jairo A., additional, and Alzate, Juan F., additional
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- 2021
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10. Influence of different stress factors during the elaboration of grape must's pieddecuve on the dynamics of yeast populations during alcoholic fermentation.
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Bedoya, Katherine, Buetas, Luis, Rozès, Nicolas, Mas, Albert, and Portillo, M. Carmen
- Subjects
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FERMENTATION , *GRAPES , *POPULATION dynamics , *SACCHAROMYCES cerevisiae , *SULFUR dioxide , *WHITE wines , *SPARKLING wines - Abstract
The pied de cuve (PdC) technique involves using a portion of grape must to undergo spontaneous fermentation, which is then used to inoculate a larger volume of must. This allows for promoting autochthonous yeasts present in the must, which can respect the typicality of the resulting wine. However, the real impact of this practice on the yeast population has not been properly evaluated. In this study, we examined the effects of sulphur dioxide (SO 2), temperature, ethanol supplementation, and time on the dynamics and selection of yeasts during spontaneous fermentation to be used as PdC. The experimentation was conducted in a synthetic medium and sterile must using a multi-species yeast consortium and in un-inoculated natural grape must. Saccharomyces cerevisiae dominated both the PdC and fermentations inoculated with commercial wine yeast, displaying similar population growth regardless of the tested conditions. However, using 40 mg/L of SO 2 and 1% (v/v) ethanol during spontaneous fermentation of Muscat of Alexandria must allowed the non- Saccharomyces to be dominant during the first stages, regardless of the temperature tested. These findings suggest that it is possible to apply the studied parameters to modulate the yeast population during spontaneous fermentation while confirming the effectiveness of the PdC methodology in controlling alcoholic fermentation. • Stress and inoculation time were analysed in pied de cuve fermentations. • Temperature, SO 2, and ethanol have strong impact on the yeast species community. • Temperature has the highest impact on fermentation rate and length. • The selected factors favour the diversity of non- Saccharomyces first and Saccharomyces at the latter. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Estudio comparativo entre los sectores vitivinícolas colombiano y chileno Caso Región Valle del Cauca (C) y Región del Maule (CH)
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Benítez Bedoya, Katherine, Montaño Caicedo, Liliana Estella, and Betancourt Guerrero, Benjamín
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Producción vitivinícola ,Valle del Cauca (Colombia) ,Estudio comparativo ,Industria vinícola - Abstract
Las culturas mundiales han tenido técnicas para la cosecha de la vid y elaboración de vino, por ende, el sector vitivinícola históricamente ha ocupado un lugar relevante en los eventos trascendentales del ser humano y en la economía mundial. Francia, Italia y España tradicionalmente han sido pioneros a nivel internacional en cultivos de vid, además son los principales productores, consumidores y exportadores de vino. No obstante, Chile ha posicionado su sector vitivinícola en los últimos años gracias a expertos en la materia que han aprovechado las condiciones edafoclimáticas y desarrollado estrategias para mejorar las técnicas de cultivo de vid; y la producción, tecnología, calidad, diversificación, precios y expansión de mercado nacional e internacional de sus vinos. Además, los empresarios de dicho sector cuentan con el apoyo del Estado chileno en diversos escenarios, situación que ha conllevado a que sean uno de los países más importantes a nivel latinoamericano en la industria del vino. Según las investigaciones realizadas para la elaboración de esta investigación, se pudo establecer que Colombia no es un país fuerte en el sector vitivinícola a nivel mundial, ni aún a nivel latinoamericano. Sin embargo, dicho sector ha contribuido en un pequeño porcentaje a la sostenibilidad de la economía del país a través de la generación de empleo y la rotación de dinero por medio de cultivos de vid, producción y comercialización de vinos nacionales. El sector vitivinícola colombiano tiene gran potencial para producir y posicionar vinos de excelente calidad tanto a nivel nacional como internacional, pero no cuenta con estudios y estrategias que contribuyan con el mejoramiento de su competitividad, razón por la cual, un estudio comparativo entre el sector vitivinícola chileno y el sector vitivinícola colombiano puede arrojar resultados positivos que ayuden a mejorar el sector Maestría MAGISTER EN ADMINISTRACIÓN
- Published
- 2015
12. Plan de negocios para el hotel ecológico de la guadua de Alcalá Valle
- Author
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Andica Gañan, Luz Andrea, Castillo Bedoya, Katherine, and Universidad del Quindío - Colombia - Director - Luz Amaris Giraldo Ocampo
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Negocio ,Hotel ,Guadua ,Ecología - Abstract
El presente trabajo consiste en elaborar un Plan de negocios para el Hotel Ecológico de la Guadua de Alcalá, Valle, teniendo en cuenta aspectos de mercadeo, un técnicos, administrativos, legales, sociales, ambientales y financieros. El Hotel Ecológico de la Guadua de Alcalá, Valle presta el servicio de alojamiento turístico rural, tiene un diseño y una arquitectura artesanal fabricada en Guadua y su estratégica ubicación geográfica lo hace participe del sector hotelero turístico rural, el cual, con la implementación de un Plan de Negocios puede mejora el área administrativa y de mercado para logra un mejor funcionamiento y aumentar la calidad en la prestación del servicio. Hoy por hoy, el turismo es uno de los principales sectores que ha estado creciendo con mayor rapidez alrededor del mundo, ya que constantemente se están desarrollando herramientas para lograr ofrecer diversas opciones que puedan satisfacer los deseos y gustos de los turistas y así alcanzar una diversificación en el mercado; por lo tanto el movimiento de turistas Nacionales e Internacionales en la región Andina es cada vez mayor, debido a la diversidad natural y cultural con la que cuenta, haciendo que la ocupación hotelera este en constante crecimiento. Para concluir, El Plan de Negocios desarrollado para El Hotel Ecológico de la Guadua de Alcalá, tiene como fin dar a conocer laimportancia de las diferentes teorías administrativas y que con el uso adecuado de estas puede mejorar los diferentes procesos hoteleros, además como con un plan de marketing el hotel puede llegar a nuevos merados, todo con la prioridad de renovar el sistema organizacional del hotel., The present paper consists on elaborating a business plan for the ecological hotel of bamboo of Alcala, Valle, taking into consideration marketing, technical, legal, managerial, social, environmental and financial aspects. The ecological hotel of bamboo of Alcala, Valle, offers the touristic hosting servicefor the rural area. The hotel has an artisanal architecture made of bamboo, and its strategic geographical location, makes the hotel an important participant in the rural hotelier zone, which with the implementation of a business plan it can be improved the administrative and marketing area to reach a better functioning and increase the quality of the services. Currently, tourism is a very important sector, which is growing up so fast around the world, because of the constant development of tools to offer a number of options that can satisfy the wishes and likes of the tourists, and then to reach a market diversification; Therefore, the national and international tourists’ movement in the Andean region is increasingly stronger due to the natural diversity and culture this region has. Thus, the hotel industry is in a constant growth. Thus, the business plan designed for the ecological hotel of Bamboo of Alcala, Valle, has as purpose to show the importance of the different administrative theories, which with an adequate use can improve the diverse hotelier processes. Besides, with a marketing plan, the hotel can reach new markets with the priority of renewing the organizational system of the hotel., Capítulo I: Anteproyecto ................................................................. 14 Capítulo II: Generalidades - Plan de negocios ....................... ....... 48 Capítulo III: Análisis de mercado .................................................... 54 Capítulo IV: Análisis técnico ........................................................... 92 Capítulo V: Análisis de la organización .......................................... 118 Capítulo VI: Análisis legal, social y ambiental ................................ 140 Capítulo VII: Análisis económico .................................................... 148 Capítulo VIII: Análisis financiero, de riesgo intangibles .................. 156 Capítulo IX: Evaluación integral del proyecto ................................. 162, Pregrado, Administrador de Negocios
- Published
- 2014
13. isolate of Encephalitozoon intestinalis from stools of a Colombian patient with AIDS
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Bedoya, Katherine, Montoya, Martha Nelly, Botero, Jorge, and Galván, Ana Luz
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microsporidiosis ,células cultivadas ,feces ,fungi ,parasitic diseases ,diarrhea ,Encephalitozoon ,patient isolate ,heces ,Acquired immunodeficiency sindrome ,síndrome de inmunodeficiencia adquirida ,cell cultures ,aislamiento de pacientes - Abstract
Introducción. Los microsporidios son agentes de infecciones oportunistas en pacientes con sida y con trasplantes, principalmente. Enterocytozoon bieneusi y Encephalitozoon intestinalis son los más frecuentes, asociados con infecciones entéricas. Los cultivos celulares han contribuido al conocimiento de los microsporidios. En Colombia no se han obtenido aislamientos provenientes de pacientes con microsporidiosis y, por consiguiente, no existen cepas autóctonas de los mismos. Objetivo. Establecer el cultivo celular de microsporidios intestinales a partir de materia fecal de pacientes parasitados. Materiales y métodos. Se realizó concentración agua-éter de la materia fecal positiva para microsporidios y el sedimento resultante se trató con una mezcla de antibióticos y antimicóticos durante 18 horas a 37 oC. Se inocularon células Vero previamente cultivadas en placas de 24 pozos y en medio RPMI con suplemento de suero bovino fetal al 10% y antibióticos, con las esporas concentradas. Los cultivos se mantuvieron a 37 oC al 5% de CO2. Se cambió de medio cada dos días y se evaluó la presencia de esporas en los sobrenadantes mediante Gram-cromótropo rápido en caliente. Resultados. En la segunda semana después de la infección, se encontraron esporas de microsporidios con morfología y coloración características. Mediante PCR se determinó que el microsporidio encontrado correspondía a la especie E. intestinalis. Conclusión. Se estableció el cultivo in vitro de microsporidios de materia fecal. Este protocolo es importante para la obtención y el mantenimiento de cepas autóctonas en Colombia, y contribuirá a las investigaciones de aspectos bioquímicos, inmunológicos y epidemiológicos de dichas cepas. Introduction. Microsporidia are obligate intracellular parasites that are recognized as important opportunistic pathogens of immunocompromised and transplanted patients. Enterocytozoon bieneusi and, less frequently, Encephalitozoon intestinalis are the most prevalent species in humans; both of them are associated with enteric infections. Cell cultures have been useful in the study of microsporidia biology. In Colombia, however, no isolates of microsporidia from patients with AIDS have been obtained. Objective. A cell culture of intestinal microsporidia was established from stools of positive patients in order to isolate a native strain. Materials and methods. Stool from a single AIDS patient was concentrated with the water-ether technique, and the sediment was treated with a mixture of antibiotics and antifungal agents for 18 hours at 37o C. Vero cells were cultivated in 24-well plates with Gibco® RPMI medium supplemented with 10% bovine fetal serum and antibiotics. The culture was subsequently inoculated with previously concentrated spores. The medium was changed every second day and the presence of spores was evaluated with the Quick Hot Gram chromotrope stain. Results. Two weeks post-infection, microsporidial spores were identified with characteristic morphology and staining properties. PCR results showed that Encephalitozoon intestinalis was the isolated species. Conclusions. A cell culture of microsporidia was established from a stool sample. This protocol is important to isolate and maintain additional native Colombian strains and it will contribute to biochemical, immunological and epidemiological studies of the currently established strain.
- Published
- 2008
14. Primer aislamiento de Encephalitozoon intestinalis a partir de muestra de materia fecal de un paciente colombiano con sida
- Author
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Galván, Ana Luz, primary, Bedoya, Katherine, additional, Montoya, Martha Nelly, additional, and Botero, Jorge, additional
- Published
- 2008
- Full Text
- View/download PDF
15. [First isolate of Encephalitozoon intestinalis from stools of a Colombian patient with AIDS].
- Author
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Bedoya K, Montoya MN, Botero J, and Galván AL
- Subjects
- Animals, Cattle, Cell Line, Colombia, Humans, Middle Aged, Acquired Immunodeficiency Syndrome parasitology, Encephalitozoon isolation & purification, Feces parasitology, Microsporidia isolation & purification
- Abstract
Introduction: Microsporidia are obligate intracellular parasites that are recognized as important opportunistic pathogens of immunocompromised and transplanted patients. Enterocytozoon bieneusi and, less frequently, Encephalitozoon intestinalis are the most prevalent species in humans; both of them are associated with enteric infections. Cell cultures have been useful in the study of microsporidia biology. In Colombia, however, no isolates of microsporidia from patients with AIDS have been obtained., Objective: A cell culture of intestinal microsporidia was established from stools of positive patients in order to isolate a native strain., Materials and Methods: Stool from a single AIDS patient was concentrated with the water-ether technique, and the sediment was treated with a mixture of antibiotics and antifungal agents for 18 hours at 37 degrees C. Vero cells were cultivated in 24-well plates with Gibco RPMI medium supplemented with 10% bovine fetal serum and antibiotics. The culture was subsequently inoculated with previously concentrated spores. The medium was changed every second day and the presence of spores was evaluated with the Quick Hot Gram chromotrope stain., Results: Two weeks post-infection, microsporidial spores were identified with characteristic morphology and staining properties. PCR results showed that Encephalitozoon intestinalis was the isolated species., Conclusions: A cell culture of microsporidia was established from a stool sample. This protocol is important to isolate and maintain additional native Colombian strains and it will contribute to biochemical, immunological and epidemiological studies of the currently established strain.
- Published
- 2008
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