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23 results on '"Behboodi, Esmail"'

3. Viable Transgenic Goats Derived from Skin Cells

Author

Behboodi, Esmail, Memili, Erdogan, Melican, David T., Destrempes, Margaret M., Overton, Susan A., Williams, Jennifer L., Flanagan, Peter A., Butler, Robin E., Liem, Hetty, Chen, Li How, Meade, Harry M., Gavin, William G., and Echelard, Yann

Published
2004
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13. Production of goats by somatic cell nuclear transfer

Author

Baguisi, Alexander, primary, Behboodi, Esmail, additional, Melican, David T., additional, Pollock, Julie S., additional, Destrempes, Margaret M., additional, Cammuso, Christine, additional, Williams, Jennifer L., additional, Nims, Scott D., additional, Porter, Catherine A., additional, Midura, Patricia, additional, Palacios, Monica J., additional, Ayres, Sandra L., additional, Denniston, Richard S., additional, Hayes, Michael L., additional, Ziomek, Carol A., additional, Meade, Harry M., additional, Godke, Robert A., additional, Gavin, William G., additional, Overström, Eric W., additional, and Echelard, Yann, additional

Published
1999
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20. Determining Acrosomal Status of the Cynomolgus Monkey (Macaca fascicularis) Sperm by Fluorescence Microscopy.

Author

Cross, Nicholas L., Morales, Patricio, Masaru Fukuda, Patricio, and Behboodi, Esmail

Subjects
SPERMATOZOA, EXOCYTOSIS, CELL physiology, FLUORESCENCE microscopy, KRA, PRIMATES
Abstract

The acrosome of Macaca fascicularis sperm cannot be distinguished by conventional light microscopy, so determining whether sperm are acrosome-intact or-reacted is difficult. We describe methods for labeling the acrosomal region of sperm with two different probes: fluoresceinated Pisum sativum agglutinin and anti-sperm antiserum. Acrosome-intact sperm are much more heavily labeled in the acrosomal region than are acrosome-reacted sperm, providing a simple means of differentiating the two types of sperm. The two probes detect similar numbers of acrosome-reacted sperm following treatment with the divalent cation ionophore, A23187. [ABSTRACT FROM AUTHOR]

Published
1989
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21. Fertility and Germline Transmission of Donor Haplotype Following Germ Cell Transplantation in Immunocompetent Goats1

Author

Honaramooz, Ali, Behboodi, Esmail, Megee, Susan O., Overton, Susan A., Galantino-Homer, Hannah, Echelard, Yann, and Dobrinski, Ina

Abstract

Transplantation of spermatogonial stem cells into syngeneic or immunosuppressed recipient mice or rats can result in donor-derived spermatogenesis and fertility. Recently, this approach has been employed to introduce a transgene into the male germline. Germ-cell transplantation in species other than laboratory rodents, if successful, holds great promise as an alternative to the inefficient methods currently available to generate transgenic farm animals that can produce therapeutic proteins in their milk or provide organs for transplantation to humans. To explore whether germ-cell transplantation could result in donor-derived spermatogenesis and fertility in immunocompetent recipient goats, testis cells were transplanted from transgenic donor goats carrying a human alpha-1 antitrypsin expression construct to the testes of sexually immature wild-type recipient goats. After puberty, sperm carrying the donor-derived transgene were detected in the ejaculates of two out of five recipients. Mating of one recipient resulted in 15 offspring, one of which was transgenic for the donor-derived transgene. This is the first report of donor cell-derived sperm production and transmission of the donor haplotype to the next generation after germ-cell transplantation in a nonrodent species. Furthermore, these results indicate that successful germ-cell transplantation is feasible between immunocompetent, unrelated animals. In the future, transplantation of genetically modified germ cells may provide a more efficient alternative for production of transgenic domestic animals.

Published
2003
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23. Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation.

Author

Honaramooz A, Behboodi E, Hausler CL, Blash S, Ayres S, Azuma C, Echelard Y, and Dobrinski I

Subjects
Animals, Busulfan pharmacology, Female, Male, Pregnancy, Spermatogenesis radiation effects, Testis drug effects, Testis radiation effects, Goats physiology, Spermatogenesis drug effects, Spermatozoa transplantation, Swine physiology
Abstract

The efficiency of germ cell transplantation, the procedure of transferring germ cells from a donor male into the testes of recipient males, can be greatly increased by reduction of endogenous germ cells in recipient animals. To develop effective methods for suppression of endogenous spermatogenesis in potential pig and goat recipients, we either administered busulfan to pregnant sows or irradiated the testes of immature goats. Piglets from sows treated twice with busulfan (7.5 mg/kg) at days 98 and 108 of gestation showed reduced gonocyte numbers at 2, 4, and 8 weeks of age and reduced initiation of spermatogenesis at 16 weeks of age. For goats, groups of 3 kids at 1, 5, or 9.5 weeks of age received fractionated irradiation of the testes with 3 doses of 2 Gy on 3 consecutive days. At 2 months after irradiation, 5%-10% of seminiferous tubule cross sections contained pachytene spermatocytes, compared with 50%-100% in controls. At 3 months after irradiation, spermatozoa appeared in 20% of tubule cross sections in all treated goats and in 100% of tubules in control goats. By 6 months after irradiation, spermatogenesis had recovered in 60% of tubules in goats treated at 5 or 9.5 weeks of age but in only 29% of tubules after treatment at 1 week of age. Therefore, late gestation in utero treatment of pigs with low doses of busulfan and testicular irradiation of goats at 1 week of age will result in a reduction in the endogenous germ cell population that could facilitate donor cell colonization after germ cell transplantation.

Published
2005
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