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Your search keyword '"Beloglazova, Natalia V."' showing total 36 results
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36 results on '"Beloglazova, Natalia V."'

19. Water-dispersed luminescent quantum dots for miRNA detection

Author

Goryacheva, Olga A., primary, Novikova, Anastasiya S., additional, Drozd, Daniil D., additional, Pidenko, Pavel S., additional, Ponomaryeva, Tatiana S., additional, Bakal, Artem A., additional, Mishra, Pradyumna K., additional, Beloglazova, Natalia V., additional, and Goryacheva, Irina Yu., additional

Published
2019
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22. Sensitive Flow-through Immunoassay for Rapid Multiplex Determination of Cereal-borne Mycotoxins in Feed and Feed Ingredients

Author

Beloglazova, Natalia V., Graniczkowska, Kinga, Njumbe Ediage, Emmanuel, Averkieva, Olga, and De Saeger, Sarah

Abstract

An easy-to-operate membrane-based flow-through test for multiplex screening of four mycotoxins (zearalenone, deoxynivalenol, aflatoxin B1, and ochratoxin A) in a variety of cereal-based feed ingredients and compound feeds, such as wheat, barley, soybean, wheat bran, rice, rice bran, maize, rapeseed meal, and sunflower meal, and various types of complete feed (duckling feed, swine feed, broiler feed, piglet feed) was developed and validated. First, the antibodies were evaluated by enzyme-linked immunosorbent assay and then employed in the membrane rapid test. The cutoff levels for zearalenone, deoxynivalenol, aflatoxin B1, and ochratoxin A were 50, 200, 1, and 10 μg/kg, respectively, based on European regulations and consumers’ requirements. As sample pretreatment, consecutive steps of extraction, dilution, solid-phase extraction by addition of C18 sorbent, and final filtration of supernatant were followed. Both the sample preparation and the analysis procedure were simple, cost-effective, and easy to perform on-site in a nonlaboratory environment. The impact of sample processing on the result of the experiment was investigated supported by experimental design. The validation procedure was performed on the basis of Commission Regulation 2006/401/EC. The numbers of false-positive and false-negative outcomes were <5%, going along with the Commission Decision 2002/657/EC. Liquid chromatography–tandem mass spectrometry was performed as a confirmatory technique.

Published
2024
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23. Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins

Author

Foubert, Astrid, Beloglazova, Natalia V., Gordienko, Anna, Tessier, Mickael D., Drijvers, Emile, Hens, Zeger, and De Saeger, Sarah

Abstract

A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 μg/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate (<5%), and the results were easy to interpret without any sophisticated equipment.

Published
2024
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35. Hydrophilic, Bright CuInS2Quantum Dotsas Cd-Free Fluorescent Labels in Quantitative Immunoassay.

Author

Speranskaya, Elena S., Beloglazova, Natalia V., Abé, Sofie, Aubert, Tangi, Smet, Philippe F., Poelman, Dirk, Goryacheva, Irina Y., De Saeger, Sarah, and Hens, Zeger

Subjects
*HYDROPHILIC compounds, *QUANTUM dots, *CADMIUM, *IMMUNOASSAY, *FLUORESCENCE, *COMPARATIVE studies
Abstract

We report on the synthesis of core–shellCuInS2/ZnS quantum dots (QDs) in organic solution, theirencapsulationwith a PEG-containing amphiphilic polymer, and the application ofthe resulting water-soluble QDs as fluorescent label in quantitativeimmunoassay. By optimizing the methods for core synthesis and shellgrowth, CuInS2/ZnS QDs were obtained with a quantum yieldof 50% on average after hydrophilization. After conjugation with anaflatoxin B1-protein derivative, the obtained QDs were used as fluorescentlabels in microplate immunoassay for the quantitative determinationof the mycotoxin aflatoxin B1. QDs-based immunoassay showed highersensitivity compared to enzyme-based immunoassay. [ABSTRACT FROM AUTHOR]

Published
2014
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36. A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M 1 and Pirlimycin Residues in Milk.

Author

Jiang W, Beloglazova NV, Luo P, Guo P, Lin G, and Wang X

Subjects
Animals, Cattle, Clindamycin analysis, Food Contamination analysis, Aflatoxin M1 analysis, Anti-Bacterial Agents analysis, Clindamycin analogs & derivatives, Drug Residues analysis, Immunoassay methods, Milk chemistry, Mycotoxins analysis, Quantum Dots chemistry
Abstract

Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M 1 and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M 1 and pirlimycin residues in milk. This multiplex frit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 μg/kg for aflatoxin M 1 and 0.5 μg/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M 1 and pirlimycin residues in milk.

Published
2017
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