19 results on '"Beltrán-Frutos, Ester"'
Search Results
2. Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy
- Author
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Martínez-Hernández, Jesús, primary, Seco-Rovira, Vicente, additional, Beltrán-Frutos, Ester, additional, Quesada-Cubo, Victor, additional, Ferrer, Concepción, additional, and Pastor, Luis Miguel, additional
- Published
- 2018
- Full Text
- View/download PDF
3. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium
- Author
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Seco-Rovira, Vicente, primary, Beltrán-Frutos, Ester, additional, Martínez-Hernández, Jesús, additional, Ferrer, Concepción, additional, and Pastor, Luis Miguel, additional
- Published
- 2017
- Full Text
- View/download PDF
4. Erratum to: The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium
- Author
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Seco-Rovira, Vicente, primary, Beltrán-Frutos, Ester, additional, Martínez-Hernández, Jesús, additional, Ferrer, Concepción, additional, and Pastor, Luis Miguel, additional
- Published
- 2017
- Full Text
- View/download PDF
5. Cellular Modifications in Spermatogenesis during Seasonal Testicular Regression: An Update Review in Mammals
- Author
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Beltrán-Frutos, Ester, primary, Seco-Rovira, Vicente, additional, Martínez-Hernández, Jesús, additional, Ferrer, Concepción, additional, Serrano-Sánchez, María Isabel, additional, and Pastor, Luis Miguel, additional
- Published
- 2022
- Full Text
- View/download PDF
6. Proliferation, apoptosis, and number of Sertoli cells in the Syrian hamster during recrudescence after exposure to short photoperiod†‡
- Author
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Martínez-Hernández, Jesús, primary, Seco-Rovira, Vicente, primary, Beltrán-Frutos, Ester, primary, Ferrer, Concepción, primary, Serrano-Sánchez, María Isabel, primary, and Pastor, Luis Miguel, primary
- Published
- 2019
- Full Text
- View/download PDF
7. Lectin-binding pattern of glycoconjugates during spontaneous testicular recrudescence in Syrian hamster (Mesocricetus auratus) after exposure to short photoperiod
- Author
-
Martínez-Hernández, Jesús, primary, Seco-Rovira, Vicente, additional, Beltrán-Frutos, Ester, additional, Ferrer, Concepción, additional, Serrano-Sánchez, María Isabel, additional, and Pastor, Luis Miguel, additional
- Published
- 2018
- Full Text
- View/download PDF
8. Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster during spontaneous recrudescence after exposure to short photoperiod
- Author
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Martínez-Hernández, Jesús, primary, Seco-Rovira, Vicente, additional, Beltrán-Frutos, Ester, additional, Ferrer, Concepción, additional, Canteras, Manuel, additional, Sánchez-Huertas, María del Mar, additional, and Pastor, Luis Miguel, additional
- Published
- 2018
- Full Text
- View/download PDF
9. Proliferation, apoptosis, and number of Sertoli cells in the Syrian hamster during recrudescence after exposure to short photoperiod†‡.
- Author
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Martínez-Hernández, Jesús, Seco-Rovira, Vicente, Beltrán-Frutos, Ester, Ferrer, Concepción, Serrano-Sánchez, María Isabel, and Pastor, Luis Miguel
- Abstract
The Sertoli cell (Sc) has been described as a quiescent cell once the animal has reached sexual maturity. Syrian hamster is an animal that displays testicular regression due to short photoperiod, during which process germ cells and Sc are removed through apoptosis. The aim of this work was to investigate histochemically whether the spontaneous testicular recrudescence processes after exposure to a short photoperiod lead to an increase in Sc proliferative activity in order to restore the normal population. Three spontaneous recrudescence groups were established: initial (IR), advanced (AR), and total (TR) recrudescence, which were compared with animal undergoing the regression process (mild: MRg, strong: SRg, and total: TRg) and animals in long photoperiod (Controls). Histological sections were submitted to histochemical techniques for detecting apoptotic and proliferative Sc with bright-field and fluorescence microscopy. For each group, the proliferative Sc index (PScI) and apoptotic Sc index (AScI), and the total number of Sc were obtained. The results revealed the existence of Vimentin+/TUNEL+ as well as Vimentin+/PCNA+ cells. The PScI was significantly higher in TRg and IR than in the other groups. The AScI was only significantly higher in MRg and SRg with respect to the other groups. The total number of Sc increased among TRg, IR, and AR, reaching values similar to those of the Controls. In conclusion, the increase in Sc proliferation from final regression and recrudescence, accompanied by a similar rate of apoptosis to the Control group, is the cause of the restoration of the Sc population during spontaneous recrudescence.
- Published
- 2020
- Full Text
- View/download PDF
10. Divergent Seasonal Reproductive Patterns in Syntopic Populations of Two Murine Species in Southern Spain, Mus spretus and Apodemus sylvaticus.
- Author
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Massoud, Diaa, Lao-Pérez, Miguel, Ortega, Esperanza, Burgos, Miguel, Jiménez, Rafael, Barrionuevo, Francisco J., Beltrán Frutos, Ester, and Pastor García, Luis Miguel
- Subjects
APODEMUS ,CLIMATE change ,SPECIES ,ANIMAL breeding ,RODENTICIDES ,ANIMAL species ,BIRD populations - Abstract
Simple Summary: In temperate zones of the Earth, most species reproduce in seasons providing the most favourable environmental conditions. Producing gametes is expensive in energetical terms, so both males and females either reduce or abolish gametogenesis during the non-breeding period. We thoroughly studied the testes of sexually inactive males of two rodents, the wood mouse, Apodemus sylvaticus, and the Algerian mouse, Mus spretus, in southern Iberian peninsula. These populations are syntopic, that is, animals of the two species share their territories and resources, so one would expect them to show similar or identical seasonal reproduction patterns. Contrarily, we found that both species reproduce during most of the year, but wood mice stop breeding in the summer whereas Algerian mice do it in winter. These divergent seasonal breeding patterns imply that either very subtle animal features and/or environmental cues operate to determine reproduction timing and support the notion that multiple models of circannual reproduction patterns are possible for different populations of the same species, showing that the mechanisms controlling seasonal reproduction are in fact very plastic and fast evolving. Hence, small mammals probably have multiple ways available to get adapted to the unstable environmental conditions derived from the ongoing global climate change. In most mammals with seasonal reproduction, males undergo testis regression during the non-breeding period. We performed a morphological, hormonal, functional, and molecular study of the testes of sexually inactive males of two species of murine rodents, the wood mouse, Apodemus sylvaticus, and the Algerian mouse, Mus spretus, in syntopic populations of southern Iberian peninsula. Both species reproduce during most of the year, but wood mice stop breeding in the summer whereas Algerian mice do it in winter. Sexually inactive males of A. sylvaticus show complete testis regression with reduced levels of serum testosterone and abnormal distribution of cell-adhesion molecules. Contrarily, inactive males of M. spretus maintain almost normal spermotogenesis despite a significant reduction of androgenic function. The lack of an evident explanation for the divergent seasonal breeding patterns found in southern populations of A. sylvaticus and M. spretus, compared with northern ones, implies that very subtle species/population-specific features and/or non-conspicuous environmental cues probably operate to determine their seasonal breeding pattern. These results also support the notion that multiple models of circannual testis variation are possible for different populations of the same species, showing that the mechanisms controlling seasonal reproduction are in fact very plastic and fast evolving. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
11. Lectin‐binding pattern of glycoconjugates during spontaneous testicular recrudescence in Syrian hamster (Mesocricetus auratus) after exposure to short photoperiod.
- Author
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Beltrán‐Frutos, Ester, Ferrer, Concepción, Serrano‐Sánchez, María Isabel, Pastor, Luis Miguel, Martínez‐Hernández, Jesús, and Seco‐Rovira, Vicente
- Abstract
Lectin histochemistry was used to characterise glycoconjugates and cellular apoptosis in the seminiferous epithelium and interstitium of hamster testis during spontaneous recrudescence. An increase in the LTA lectin affinity was observed in spermatids in the Golgi phase. An increase in labelling of PNA and Con‐A lectin in acrosome of spermatids (acrosome phase) as well as increased labelling with Con‐A in spermatids (cap phase) was observed. Spermatocytes showed decreased affinity with PNA and AAA lectins and an increase in positivity for LTA and GNA lectins. Spermatogonia showed a slight decrease in positivity to WGA and an increase in labelling with Con‐A and a decreased affinity for the AAA lectin. At the end of recrudescence, all these germinal cells showed a similar pattern to the control. The Sertoli cells showed a gradual decrease in labelling with the GNA lectin and the Leydig cells an increase in labelling with Con‐A and GNA. Particularly unusual was the observation of apoptotic spermatocytes and spermatids positive for PNA, GNA, AAA and Con‐A, together with spermatocytes positive to LTA. In conclusion, the normal lectin pattern is recovered during testis recrudescence and germ cell apoptotic activity is low, as is observed by specific lectins for germ cells in apoptosis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
12. Alteraciones histológicas debidas al envejecimiento en el tútbulo seminífero del hamster sirio (Mesocricetus auratus) cambios en la proliferación celular
- Author
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Horn Ureña, Ramón, Pastor García, Luis Miguel, Beltrán Frutos, Esther, Departamento de Biología Celular e Histología, Beltrán Frutos, Ester, and Universidad de Murcia. Departamento de Biología Celular
- Subjects
Ciencias ,Genitales masculinos ,Envejecimiento-Aspectos fisiológicos ,Histología ,611 - Anatomía ,Animales-Reproducción ,636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos - Abstract
Un área muy actual de investigación es el estudio de las causas del envejecimiento. En el ámbito de la biología reproductiva también lo está siendo por sus implicaciones tanto en la medicina como en la veterinaria. Si durante muchos años los estudios se han centrado en la mujer o en las hembras para explicar el descenso reproductivo con la edad actualmente se está prestando más atención al factor masculino. Estos estudios abordan varios aspectos: cambios endocrinos, alteraciones de los espermatozoides, trasmisión de enfermedades hereditarias, o la necesidad de terapias sustitutivas de testosterona en la vejez. Respecto a los cambios histológicos del testículo envejecido, se han realizado diversos estudios desde hace décadas, pero han dejado ciertas cuestiones sin aclarar y se han centrado en el hombre y en la rata. Junto a esto, durante los últimos años, diversos autores han propuesto que el mecanismo tisular implicado en la progresiva disminución de la espermatogénesis con la edad, está relacionado con cambios en el equilibrio entre la proliferación/apoptosis en el epitelio seminífero. En la presente tesis doctoral nos hemos propuesto por un lado, realizar un completo análisis histológico tanto cualitativo como cuantitativo de los cambios que acontecen con la edad en el epitelio seminífero y pared tubular del hámster sirio. Por otro, hemos investigado también si la proliferación está o no alterada en este epitelio relacionándola con las alteraciones histológicas observadas en el mismo. Para ello, utilizamos hámsteres machos de 6, 12 y 24 meses de edad. Los testículos fueron fijados y procesados para microscopia de luz convencional e inmunohistoquimica y, electrónica de transmisión. Se realizó un estudio semicuantitativo del tipo histológico de degeneración de las secciones de los túbulos seminíferos determinando el grado de progresiva alteración de su epitelio con la edad. Inmunohistoquímicamente se identificó la inmunoreactividad a laminina y fibronectina en la pared tubular y las células en proliferación. Morfométricamente se calculó una serie de parámetros testiculares y el volumen total de inmunoreactividad a fibronectina y laminina. Respecto a la proliferación se calculó el índice total de proliferación como el número de células proliferando por tipo histológico de sección tubular. Por último, con las secciones ultrafinas se realizó una descripción ultrarestructural del epitelio seminífero y se calculó el grosor de la pared tubular. En los animales envejecidos se observó un incremento significativo de las secciones tubulares con diversas alteraciones del epitelio seminífero especialmente hipoespermatogénesis y detención de la espermatogénesis. El volumen del testículo, el diámetro y volumen del túbulo como el volumen de epitelio del túbulo seminífero sufrieron un descenso significativo con la edad, por el contario la longitud del túbulo se incrementó. El volumen de fibronectina y laminina aumento en los animales viejos siendo el índice de proliferación menor en estos animales. El número de células en proliferación por tipo histológico de sección de túbulo seminífero fue progresivamente y significativamente menor en las secciones alteradas respecto a las secciones normales. No se observó diferencias significativas respecto a la edad entre tipos histológicos de sección de túbulo seminífero. Ultraestructuralmente se observó alteraciones citológicas importantes en la célula de Sertoli así como en el proceso de espermiogénesis. La pared presentó un engrosamiento significativo tanto en los túbulos normales como en los hipoespermatogénicos o en detención de la espermatogénesis. En conclusión, el envejecimiento del túbulo seminífero del hámster supone una pérdida progresiva de la actividad espermatogénica que está relacionada con cambios del túbulo y de su pared. Estos cambios no se producen de forma uniforme en todo él y se asocian a una disminución de la proliferación celular en sus zonas alteradas histológicamente. Éstas presentan además células de Sertoli modificadas ultraestructuralmente., A very current area of research is the study of the causes of aging. In the field of reproductive biology the aging is important by their implications both in medicine and veterinary. If for many years the studies have focused on women or females to explain the reproductive decline with age it is now being paid more attention to male factor. These studies address several aspects: endocrine changes, abnormal sperm, transmission of hereditary diseases, or the need for testosterone replacement therapy in older age. Regarding the histological changes of aging testes, they have conducted several studies for decades, but have left some issues unclear and have focused on humans and rats. Alongside this, in recent years, several authors have proposed that the mechanism involved in decrease of spermatogenesis with age, is related to changes in the balance between proliferation / apoptosis in the seminiferous epithelium. In this thesis we have proposed on the one hand, a complete histological analysis of qualitative and quantitative changes that occur with age in the seminiferous epithelium and tubular wall of the Syrian hamster. Furthermore, we have also investigated whether this proliferation in the epithelium altered is linked with the histological changes observed in it. To do this, we use male hamsters 6, 12 and 24 months old. The testes were fixed and processed for conventional light microscopy, immunohistochemistry and transmission electron microscopy. A semiquantitative histological study of sections of seminiferous tubules for determining the degree of gradual deterioration with age, in its epithelium was performed with age. Immunoreactivity to laminin and fibronectin in the tubular wall and proliferating cells was identified. Morphometrically a series of testicular parameters and the total volume of fibronectin and laminin immunoreactivity was calculated. The total proliferation index as the number of proliferating cells by histologic type of tubular section was calculated. Finally, with ultrathin sections, an ultrarestructural description of seminiferous epithelium, and thickness determination of tubular wall was performed. In aged animals a significant increase in the tubular sections with various disorders especially hypospermatogenesis of seminiferous epithelium and arrest of spermatogenesis was observed. The testicular volume, diameter and volume as epithelial volume of seminiferous tubule suffered a significant decrease with age, on the contrary an increased tubule length was observed. An increase in fibronectin and laminin volume in old animals and less proliferation rate in these animals was determined. The number of proliferating cells per histologic section of seminiferous type tubule, progressively and significantly decrease in altered sections respect to normal sections. No significant differences respect to age between histological types sections of seminiferous tubule was observed. Ultrastructurally important cytological changes were observed in the Sertoli cell and the process of spermiogenesis. The wall thickening showed an increase significant in normal, hipospermatogenic and spermatogenesis arrest tubules. The number of proliferating cells per histologic section of seminiferous type tubule, progressively and significantly decrease in altered sections respect to normal sections. In conclusion, the aging of the seminiferous tubule hamster suppose a loss of spermatogenic activity which is related to changes in the tubule and its wall. These changes do not occur uniformly throughout the tubule and they associated with a decrease in cell proliferation in altered tubular areas. These areas also have Sertoli cells with a modified ultrastructure.
- Published
- 2018
13. Alteraciones histológicas debidas al envejecimiento en el túbulo seminífero del hámster sirio (mesocricetus auratus)
- Author
-
Horn Ureña, Ramón, Pastor García, Luis Miguel, Beltrán Frutos, Ester, and Departamento de Biología Celular e Histología
- Subjects
Genitales masculinos ,Histología ,611 - Anatomía ,Aspectos fisiológicos del envejecimiento ,636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos ,Reproducción en animales - Abstract
Un área muy actual de investigación es el estudio de las causas del envejecimiento. En el ámbito de la biología reproductiva también lo está siendo por sus implicaciones tanto en la medicina como en la veterinaria. Si durante muchos años los estudios se han centrado en la mujer o en las hembras para explicar el descenso reproductivo con la edad actualmente se está prestando más atención al factor masculino. Estos estudios abordan varios aspectos: cambios endocrinos, alteraciones de los espermatozoides, trasmisión de enfermedades hereditarias, o la necesidad de terapias sustitutivas de testosterona en la vejez. Respecto a los cambios histológicos del testículo envejecido, se han realizado diversos estudios desde hace décadas, pero han dejado ciertas cuestiones sin aclarar y se han centrado en el hombre y en la rata. Junto a esto, durante los últimos años, diversos autores han propuesto que el mecanismo tisular implicado en la progresiva disminución de la espermatogénesis con la edad, está relacionado con cambios en el equilibrio entre la proliferación/apoptosis en el epitelio seminífero. En la presente tesis doctoral nos hemos propuesto por un lado, realizar un completo análisis histológico tanto cualitativo como cuantitativo de los cambios que acontecen con la edad en el epitelio seminífero y pared tubular del hámster sirio. Por otro, hemos investigado también si la proliferación está o no alterada en este epitelio relacionándola con las alteraciones histológicas observadas en el mismo. Para ello, utilizamos hámsteres machos de 6, 12 y 24 meses de edad. Los testículos fueron fijados y procesados para microscopia de luz convencional e inmunohistoquimica y, electrónica de transmisión. Se realizó un estudio semicuantitativo del tipo histológico de degeneración de las secciones de los túbulos seminíferos determinando el grado de progresiva alteración de su epitelio con la edad. Inmunohistoquímicamente se identificó la inmunoreactividad a laminina y fibronectina en la pared tubular y las células en proliferación. Morfométricamente se calculó una serie de parámetros testiculares y el volumen total de inmunoreactividad a fibronectina y laminina. Respecto a la proliferación se calculó el índice total de proliferación como el número de células proliferando por tipo histológico de sección tubular. Por último, con las secciones ultrafinas se realizó una descripción ultrarestructural del epitelio seminífero y se calculó el grosor de la pared tubular. En los animales envejecidos se observó un incremento significativo de las secciones tubulares con diversas alteraciones del epitelio seminífero especialmente hipoespermatogénesis y detención de la espermatogénesis. El volumen del testículo, el diámetro y volumen del túbulo como el volumen de epitelio del túbulo seminífero sufrieron un descenso significativo con la edad, por el contario la longitud del túbulo se incrementó. El volumen de fibronectina y laminina aumento en los animales viejos siendo el índice de proliferación menor en estos animales. El número de células en proliferación por tipo histológico de sección de túbulo seminífero fue progresivamente y significativamente menor en las secciones alteradas respecto a las secciones normales. No se observó diferencias significativas respecto a la edad entre tipos histológicos de sección de túbulo seminífero. Ultraestructuralmente se observó alteraciones citológicas importantes en la célula de Sertoli así como en el proceso de espermiogénesis. La pared presentó un engrosamiento significativo tanto en los túbulos normales como en los hipoespermatogénicos o en detención de la espermatogénesis. En conclusión, el envejecimiento del túbulo seminífero del hámster supone una pérdida progresiva de la actividad espermatogénica que está relacionada con cambios del túbulo y de su pared. Estos cambios no se producen de forma uniforme en todo él y se asocian a una disminución de la proliferación celular en sus zonas alteradas histológicamente. Éstas presentan además células de Sertoli modificadas ultraestructuralmente. A very current area of research is the study of the causes of aging. In the field of reproductive biology the aging is important by their implications both in medicine and veterinary. If for many years the studies have focused on women or females to explain the reproductive decline with age it is now being paid more attention to male factor. These studies address several aspects: endocrine changes, abnormal sperm, transmission of hereditary diseases, or the need for testosterone replacement therapy in older age. Regarding the histological changes of aging testes, they have conducted several studies for decades, but have left some issues unclear and have focused on humans and rats. Alongside this, in recent years, several authors have proposed that the mechanism involved in decrease of spermatogenesis with age, is related to changes in the balance between proliferation / apoptosis in the seminiferous epithelium. In this thesis we have proposed on the one hand, a complete histological analysis of qualitative and quantitative changes that occur with age in the seminiferous epithelium and tubular wall of the Syrian hamster. Furthermore, we have also investigated whether this proliferation in the epithelium altered is linked with the histological changes observed in it. To do this, we use male hamsters 6, 12 and 24 months old. The testes were fixed and processed for conventional light microscopy, immunohistochemistry and transmission electron microscopy. A semiquantitative histological study of sections of seminiferous tubules for determining the degree of gradual deterioration with age, in its epithelium was performed with age. Immunoreactivity to laminin and fibronectin in the tubular wall and proliferating cells was identified. Morphometrically a series of testicular parameters and the total volume of fibronectin and laminin immunoreactivity was calculated. The total proliferation index as the number of proliferating cells by histologic type of tubular section was calculated. Finally, with ultrathin sections, an ultrarestructural description of seminiferous epithelium, and thickness determination of tubular wall was performed. In aged animals a significant increase in the tubular sections with various disorders especially hypospermatogenesis of seminiferous epithelium and arrest of spermatogenesis was observed. The testicular volume, diameter and volume as epithelial volume of seminiferous tubule suffered a significant decrease with age, on the contrary an increased tubule length was observed. An increase in fibronectin and laminin volume in old animals and less proliferation rate in these animals was determined. The number of proliferating cells per histologic section of seminiferous type tubule, progressively and significantly decrease in altered sections respect to normal sections. No significant differences respect to age between histological types sections of seminiferous tubule was observed. Ultrastructurally important cytological changes were observed in the Sertoli cell and the process of spermiogenesis. The wall thickening showed an increase significant in normal, hipospermatogenic and spermatogenesis arrest tubules. The number of proliferating cells per histologic section of seminiferous type tubule, progressively and significantly decrease in altered sections respect to normal sections. In conclusion, the aging of the seminiferous tubule hamster suppose a loss of spermatogenic activity which is related to changes in the tubule and its wall. These changes do not occur uniformly throughout the tubule and they associated with a decrease in cell proliferation in altered tubular areas. These areas also have Sertoli cells with a modified ultrastructure.
- Published
- 2016
14. HSP47 expression in the hamster Sertoli cell: An immunohistochemical study.
- Author
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Seco-Rovira V, Serrano-Sánchez MI, Beltrán-Frutos E, Martínez-Hernández J, Ferrer C, and Pastor LM
- Subjects
- Male, Animals, Cricetinae, Mesocricetus, Seminiferous Tubules metabolism, Seminiferous Tubules pathology, Testis metabolism, Testis pathology, Aging metabolism, Aging pathology, Photoperiod, Sertoli Cells metabolism, Sertoli Cells pathology, HSP47 Heat-Shock Proteins metabolism, Spermatogenesis physiology, Immunohistochemistry
- Abstract
HSP47, a chaperone whose main function is the maturation of collagen molecules, is considered a marker of fibrotic diseases. Increased collagen synthesis in the testis has been associated with various pathologies leading to seminiferous tubule regression. Our aim was to study whether HSP47 is expressed in hamster Sertoli cells both in the adult and in two physiological situations of seminiferous tubule atrophy: irreversible testicular ageing and testicular regression due to short photoperiod (reversible). Eighteen animals were divided as follows: a group of 6 young animals aged 6 months, a group of 6 animals aged 24 months, which were exposed to a long photoperiod, and a final group of 6 young animals subjected to a short photoperiod. Testicular samples were fixed in methacarn and an immunohistochemical technique was used to detect HSP47. A semiquantitative study of of this protein expresion was performed between tubular sections of aged animals with complete spermatogenesis and arrested spermatogenesis and tubular sections with arrest spermatogenesis of photoinhibited testes. Sertoli cells were positive for HSP47, the intensity being greater in tubular sections with arrested spermatogenesis in both aged and photoinhibited animals. Semiquantitative analysis corroborated this observation in the sense that the expression of this protein differed according to the functional state of the seminiferous tubules. Thus, the radio of immunoreactivity was significantly higher in tubular sections with arrested spermatogenesis in aged animals compared with regressed animals, and in the latter compared with those whose tubular sections showed complete spermatogenesis. In conclusion, HSP47 expression in Sertoli cells was found for the first time in mammals. Moreover, increased expression seemed to be related to the degree of atrophy of the seminiferous epithelium and to the reversible or non-reversible physiological state of the seminiferous epithelium., (©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.)
- Published
- 2024
- Full Text
- View/download PDF
15. Heterogeneity of mesenchymal cells in human amniotic membrane at term.
- Author
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Cortes-Sandoval S, Seco-Rovira V, Beltrán-Frutos E, Serrano-Sánchez MI, Martínez-Hernández J, Ferrer C, Delgado JL, Insausti CL, Blanquer M, and Pastor LM
- Subjects
- Humans, Female, Pregnancy, Microscopy, Electron, Transmission, Cell Differentiation physiology, Amnion cytology, Mesenchymal Stem Cells cytology
- Abstract
There is increasing interest in understanding the tissue biology of human amniotic membrane (hAM) given its applications in medicine. One cellular component is mesenchymal cells, which can be extracted, cultured and differentiated " in vitro " into various cell types. These studies show that there is heterogeneity among mesenchymal cells. The aim of this work is to study the membrane in situ to determine whether this cellular heterogeneity exists. The hAMs were obtained from caesarean deliveries at term and analyzed by histological techniques. Types I-III mesenchymal cells and Hofbauer were distinguished by light microscopy. Histochemically, mesenchymal cell types showed successively increasing positivity to: PAS, vimentin, fibronectin, and Concanavalin-A; VGEF, TGF-β2, PDGF-C, FGF-2. By the semiquantitative point of view, the percentage of Type II cells was 60%, significantly higher than the other types. With transmission electron microscopy, an intermediate cell type between II-III was observed. Strong vesiculation of the rough endoplasmic reticulum (RER) with exocytosis was observed. In addition, an accumulation of a similar material to the extracellular matrix in the RER caused its dilation especially in type III
TEM cells. Some of this material acquired a globular structure. These structures were also found free in the extracellular matrix. In conclusion, the mesenchymal cells of the fibroblastic layer of the hAMs studied are heterogeneous, with some undifferentiated and others with a probably senescent fibroblastic phenotype with accumulation in their RER of fibronectin. These results may be of interest to extract mesenchymal cells from hAMs for use in regenerative medicine and to better understand the mechanisms of fetal membrane rupture., (©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.)- Published
- 2024
- Full Text
- View/download PDF
16. Lectin-binding pattern of glycoconjugates during spontaneous testicular recrudescence in Syrian hamster (Mesocricetus auratus) after exposure to short photoperiod.
- Author
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Martínez-Hernández J, Seco-Rovira V, Beltrán-Frutos E, Ferrer C, Serrano-Sánchez MI, and Pastor LM
- Subjects
- Acrosome metabolism, Animals, Cricetinae, Male, Mesocricetus, Recurrence, Seminiferous Epithelium metabolism, Spermatids metabolism, Apoptosis physiology, Glycoconjugates metabolism, Lectins metabolism, Photoperiod, Testis metabolism
- Abstract
Lectin histochemistry was used to characterise glycoconjugates and cellular apoptosis in the seminiferous epithelium and interstitium of hamster testis during spontaneous recrudescence. An increase in the LTA lectin affinity was observed in spermatids in the Golgi phase. An increase in labelling of PNA and Con-A lectin in acrosome of spermatids (acrosome phase) as well as increased labelling with Con-A in spermatids (cap phase) was observed. Spermatocytes showed decreased affinity with PNA and AAA lectins and an increase in positivity for LTA and GNA lectins. Spermatogonia showed a slight decrease in positivity to WGA and an increase in labelling with Con-A and a decreased affinity for the AAA lectin. At the end of recrudescence, all these germinal cells showed a similar pattern to the control. The Sertoli cells showed a gradual decrease in labelling with the GNA lectin and the Leydig cells an increase in labelling with Con-A and GNA. Particularly unusual was the observation of apoptotic spermatocytes and spermatids positive for PNA, GNA, AAA and Con-A, together with spermatocytes positive to LTA. In conclusion, the normal lectin pattern is recovered during testis recrudescence and germ cell apoptotic activity is low, as is observed by specific lectins for germ cells in apoptosis., (© 2018 Blackwell Verlag GmbH.)
- Published
- 2019
- Full Text
- View/download PDF
17. Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy.
- Author
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Martínez-Hernández J, Seco-Rovira V, Beltrán-Frutos E, Quesada-Cubo V, Ferrer C, and Pastor LM
- Subjects
- Animals, Apoptosis, Cell Differentiation, Cell Proliferation, Male, Mesocricetus, Microscopy, Confocal methods, Microscopy, Fluorescence methods, Sertoli Cells cytology, Sertoli Cells pathology
- Abstract
Sertoli cells, the testicular somatic cells of the seminiferous epithelium, are vital for the survival of the epithelium. They undergo proliferation and apoptosis during fetal, neonatal, and prepubertal development. Apoptosis is increased in certain situations such as exposure to many substances, for example, toxics, or short photoperiod in the non-breeding season of some mammals. Therefore, it has always been considered that Sertoli cells that reach adulthood are quiescent cells, that is to say, nonproliferative, do not die, are terminally differentiated, and whose numbers remain constant. Recently, a degree of both proliferation and apoptosis has been observed in normal adult conditions, suggesting that consideration of this cell as quiescent may be subject to change. All this make it necessary to use histochemical techniques to demonstrate whether Sertoli cells are undergoing proliferation or apoptosis in histological sections and to allow the qualitative and quantitative study of these. In this chapter, we present two double-staining techniques that can be used for identifying Sertoli cells in proliferation or apoptosis by fluorescence microscopy. In both, the Sertoli cells are identified by an immunohistochemistry for vimentin followed by an immunohistochemistry for PCNA or a TUNEL histochemistry.
- Published
- 2018
- Full Text
- View/download PDF
18. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium.
- Author
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Seco-Rovira V, Beltrán-Frutos E, Martínez-Hernández J, Ferrer C, and Pastor LM
- Subjects
- Animals, Biomarkers, Cricetinae, Germ Cells metabolism, Glycoconjugates, In Situ Nick-End Labeling methods, Male, Sensitivity and Specificity, Testis cytology, Testis metabolism, Apoptosis, Histocytochemistry methods, Lectins metabolism, Seminiferous Epithelium metabolism
- Abstract
Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.
- Published
- 2017
- Full Text
- View/download PDF
19. Erratum to: The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium.
- Author
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Seco-Rovira V, Beltrán-Frutos E, Martínez-Hernández J, Ferrer C, and Pastor LM
- Published
- 2017
- Full Text
- View/download PDF
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