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2 results on '"Ben Farrar"'

1. Locating and Activating Molecular 'Time Bombs': Induction of Mycolata Prophages.

Author

Zoe A Dyson, Teagan L Brown, Ben Farrar, Stephen R Doyle, Joseph Tucci, Robert J Seviour, and Steve Petrovski

Subjects
Medicine, Science
Abstract

Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. Bioinformatic analysis using the PHAge Search Tool (PHAST) on Mycolata whole genome sequence data in GenBank for members of the genera Gordonia, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella revealed 83% contained putative prophage DNA sequences. Subsequent prophage inductions using mitomycin C were conducted on 17 Mycolata strains. This led to the isolation and genome characterization of three novel Caudovirales temperate phages, namely GAL1, GMA1, and TPA4, induced from Gordonia alkanivorans, Gordonia malaquae, and Tsukamurella paurometabola, respectively. All possessed highly distinctive dsDNA genome sequences.

Published
2016
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2. Locating and activating molecular 'time bombs': induction of Mycolata prophages

Author

Teagan L. Brown, Stephen R. Doyle, Joseph Tucci, Robert J. Seviour, Steve Petrovski, Zoe A. Dyson, and Ben Farrar

Subjects
0301 basic medicine, Tsukamurella, Prophages, lcsh:Medicine, Protein Sequencing, medicine.disease_cause, Genome, Nocardia, Mycolic acid, Database and Informatics Methods, Rhodococcus, Bacteriophages, Gordonia Bacterium, lcsh:Science, Uncategorized, chemistry.chemical_classification, Genetics, Viral Genomics, Multidisciplinary, Sewage, Chromosome Mapping, Genomics, Genomic Databases, Gordonia malaquae, Viruses, Sequence Analysis, Research Article, food.ingredient, Sequence Databases, Genome, Viral, Microbial Genomics, Biology, Gordonia, Research and Analysis Methods, Microbiology, Evolution, Molecular, 03 medical and health sciences, food, Caudovirales, Sequence Motif Analysis, Virology, Actinomycetales, medicine, Molecular Biology Techniques, Sequencing Techniques, Molecular Biology, DNA sequence analysis, Prophage, lcsh:R, Organisms, Biology and Life Sciences, Computational Biology, Genome Analysis, biology.organism_classification, Mutagenesis, Insertional, Biological Databases, 030104 developmental biology, chemistry, Virus Activation, lcsh:Q
Abstract

Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. Bioinformatic analysis using the PHAge Search Tool (PHAST) on Mycolata whole genome sequence data in GenBank for members of the genera Gordonia, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella revealed 83% contained putative prophage DNA sequences. Subsequent prophage inductions using mitomycin C were conducted on 17 Mycolata strains. This led to the isolation and genome characterization of three novel Caudovirales temperate phages, namely GAL1, GMA1, and TPA4, induced from Gordonia alkanivorans, Gordonia malaquae, and Tsukamurella paurometabola, respectively. All possessed highly distinctive dsDNA genome sequences.

Published
2023
Full Text
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