78 results on '"Benaglia, G."'
Search Results
2. Characterization of three sialidases from Danio rerio
- Author
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Forcella, M, Manzoni, M, Benaglia, G, Bonanomi, M, Giacopuzzi, E, Papini, N, Bresciani, R, Fusi, P, Borsani, G, Monti, E, Forcella M., Manzoni M., Benaglia G., Bonanomi M., Giacopuzzi E., Papini N., Bresciani R., Fusi P., Borsani G., Monti E., Forcella, M, Manzoni, M, Benaglia, G, Bonanomi, M, Giacopuzzi, E, Papini, N, Bresciani, R, Fusi, P, Borsani, G, Monti, E, Forcella M., Manzoni M., Benaglia G., Bonanomi M., Giacopuzzi E., Papini N., Bresciani R., Fusi P., Borsani G., and Monti E.
- Abstract
Zebrafish encodes several sialidases belonging to the NEU3 group, the plasma membrane-associated member of the family with high specificity toward ganglioside substrates. Neu3.1, Neu3.2 and Neu 3.3 have been expressed in E. coli and purified using the pGEX-2T expression system. Although all the enzymes are expressed by bacterial cells, Neu3.1 formed insoluble aggregates that hampered its purification. Neu3.2 and Neu3.3 formed oligomers as demonstrated by gel filtration chromatography experiments. Actually, the first formed a trimer whereas the second a pentamer. Intriguingly, despite relevant degree of sequence identity and similarity, the two enzymes showed peculiar substrate specificities toward gangliosides other than GM3, two glycoproteins and two forms of sialyllactose. Using molecular modelling and the crystal structure of the human cytosolic sialidase NEU2 as a template, the 3D models of the sialidases from zebrafish have been generated. As expected, the 3D models showed the typical six blade beta-propeller typical of sialidases, with an overall highly conserved active site architecture. The differences among the three zebrafish enzymes and human NEU2 are mainly located in the loops connecting the antiparallel beta strands of the propeller core. These portions of the proteins are probably responsible for the differences observed in substrate specificities, as well as in the different subcellular localization and aggregation features observed in solution. Finally, the in silico analysis of RNA-Seq data evidenced a peculiar expression profile of the three genes during embryogenesis, suggesting different roles of these sialidases during development.
- Published
- 2021
3. Poliomyelitis Immunity Status at Different Intervals from Vaccination
- Author
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Bellelli, E., Bracchi, U., Tanzi, M. L., Benaglia, G., and Montanarini, G.
- Published
- 1986
4. Duration of ruptured membranes and vertical transmission of HIV-1: a meta-analysis from 15 prospective cohort studies
- Author
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Bulterys, M. B., Fowler, M. G., Hanson, I. C., Lemay, M., Mayaux, M. J., Mofenson, L., Newell, M. -L., Peavy, H., Peckham, C., Read, J. S., Rother, C., Simpson, B. J., Van Dyke, R. B., Harris, D. R., Peavy, H. H., Easley, K., Khammy, A., Nugent, R. P., Mitchell, R., Owen, W., Van Dyke, R., Widmayer, S., Bardeguez, A., Hanson, C., Wiznia, A., Luzuriaga, K., Viscarello, R., Ho, D., Koup, R., Chen, I., Krogstad, P., Mullins, J., Wolinsky, S., Korber, B., Walker, B., Ammann, A., Clapp, S., Mcdonald, D., Lapointe, N., Boucher, M., Fauvel, M., Hankins, C., Samson, J., Newell, M. L., Peckham, C. S., Thorne, C. N., Giaquinto, C., Ruga, E., De Rossi, A., Truscia, D., Grosch-Worner, I., Schafer, A., Mok, J., Johnstone, F., Jiminez, J., de Alba, C., Garcia Rodriguez, M. C., Bates, I., de Josee, I., Hawkins, F., Martinez Zapico, R., Pena, J. M., Gonzalez Garcia, J., Arribas Lopez, J. R., Asensi-Botet, F., Otero, M. C., Peerez-Tamarit, D., Moya, A., Galbis, M. J., Scherpbier, H., Boer, K., Bohlin, A. B., Lindgren, S., Anzen, B., Belfrage, E., Lidin-Jansson, G., Levy, J., Barlow, P., Hainaut, M., Peltier, A., Ferrazin, A., De Maria, A., Gotta, C., Mur, A., Vinolas, M., Paya, A., Loepez-Vilchez, M. A., Coll, O., Fortuny, C., Boguna, J., Casellas Caro, M., Canet, Y., Pardi, G., Ravizza, M., Semprini, E., Castagna, C., Fiore, S., Guerra, B., Lanari, M., Bianchi, S., Bovicelli, L., Prati, E., Zanelli, S., Duse, M., Soresina, A., Scaravelli, G., Stegagno, M., De Santis, M., Muggiasca, M. L., Vigano, A., Spinillo, A., Ravagni Probizer, F., Bucceri, A., Rancilio, L., Taylor, G. P., Lyall, H., Penn, Z., Blott, M., Valerius, N. H., Martinelli, P., Buffolano, W., Tibaldi, C., Ziarati, N., Semprini, A., Della Torre, M., Parazzini, F., Dallacasa, P., Bianchi, U., Pachi, A., Mancuso, S., Villa, P., Conti, M., Principi, N., Muggiasca, M., Marchisio, P., Zara, C., Ravagni, F., Vignali, M., Rossi, G., Selvaggi, L., Greco, P., Vimercati, A., Massi, G., Innocenti, T., Fiscella, A., Sansone, M., Benedetto, C., Tadrist, B., Thevenieau, D., Gondry, J., Paulard, B., Alisy, C., Brault, D., Tordjeman, N., Mamou, J., Rozan, M., Colombani, D., Pincemaille, O., Salvetti, A., Chabanier, C., Hernandorena, X., Leroy, J., Schaal, J., Balde, P., Faucher, P., Lachassinne, E., Benoit, S., Douard, D., Hocke, C., Barjot, P., Brouard, J., Delattre, P., Stien, L., Audibert, F., Labrune, P., Vial, M., Mazy, F., Sitbon, D., Crenn-Hebert, C., Floch-Tudal, C., Akakpo, R., Daveau, C., Leblanc, A., Cesbron, P., Duval-Arnould, M., Huraux-Rendu, C., Lemerle, S., Touboul, C., Guerin, M., Maingueneau, C., Reynaud, I., Rousseau, T., Ercoil, V., Lanza, M., Denavit, M., Garnier, J., Lahsinat, K., Pia, P., Allouche, C., Nardou, M., Grall, F., May, A., Dallot, M., Lhuillier, P., Cecile, W., Mezin, R., Bech, A., Lobut, J., Algava, G., Chalvon Dermesay, A., Busuttil, R., Jacquemot, M., Bader-Meunier, B., Fridman, S., Codaccioni, X., Maxingue, F., Thomas, D., Alain, J., De Lumley, L., Tabaste, J., Bailly Salin, P., Seaume, H., Guichard, A., Kebaill, K., Roussouly, C., Botto, C., De Lanete, A., Wipff, P., Cravello, L., De Boisse, P., Leclaire, M., Michel, G., Crumiere, C., Lefevre, V., Le Lorier, B., Pauly, I., Robichez, B., Seguy, D., Delhinger, M., Rideau, F., Talon, P., Benos, P., Huret, C., Nicolas, J., Heller-Roussin, B., Saint-Leger, S., Delaporte, M., Hubert, C., De Sarcus, B., Karoubi, P., Mechinaud, F., Bertcrottiere, D., Bongain, A., Monpoux, F., De Gennes, C., Devianne, F., Nisand, I., Rousset, M., Mouchnino, G., Muray, J., Munzer, M., Quereux, C., Brossard, V., Clavier, B., Allemon, M., Rotten, D., Stephan, J., Varlet, M., Guyot, B., Narcy, P., Bardinet, F., De Caunes, F., Jeny, R., Robin, M., Raison Boulley, A., Savey, L., Berrebi, A., Tricoire, J., Borderon, J., Fignon, A., Guillot, F., Maria, B., Broyard, A., Chitrit, Y., Firtion, G., Mandelbrot, L., Lafay Pillet, M., Parat, S., Boissinot, C., Garec, N., Levine, M., Ottenwalter, A., Schaller, F., Vilmer, E., Courpotin, C., Brunner, C., Ciraru-Vigneron, N., Hatem-Gantzer, G., Fritel, X., Wallet, A., Bouille, J., Milliez, J., Bensaid Mrejen, D., Dermer, E., Noseda, G., Bardou, D., Cressaty, J., Francoual, C., Carlus Moncomble, C., Cohen, H., Blanche, S., Bastion, H., Benifla, J., Benkhatar, F., Berkane, N., Hervee, F., Ronzier, M., Mayaux, Mj., de Martino, M., Tovo, P. -A., Galli, L., Gabiano, C., Ferraris, G., Garetto, S., Palomba, E., Riva, C., Vierucci, A., de Luca, M., Farina, S., Fundaro, C., Genovese, O., Mereu, G., Forni, G. L., Casadei, A., Zuccotti, G. V., Riva, E., Cellini, M., Baraldi, C., Consolini, R., Palla, G., Ruggeri, M., Ciccimarra, F., Guarino, A., Osimani, P., Benaglia, G., Romano, A., De Mattia, D., Caselli, D., Boni, S., Dell'Erba, G., Bassanetti, F., Sticca, M., Timpano, C., Magnani, C., Salvatore, C., Lipreri, R., Tornaghi, R., Pinzani, R., Cecchi, M. T., Bezzi, T., Battisti, L., Bresciani, E., Castelli Gattinara, G., Nasi, C., Pellegatta, A., Mazza, A., Baldi, F., Altobelli, R., Deiana, M., Colnaghi, C., Tarallo, L., Tondo, U., Anastasio, E., Chiriaco, P. G., Ruggeri, C., Scott, G., Hutto, C., O'Sullivan, M., Malmsberry, A., Willoughby, A., Burns, D., Goedert, J., Landesman, S., Minkoff, H., Mendez, H., Holman, S., Rubinstein, A., Durako, S., Muenz, L., Goodwin, S., Bryson, Y., Dillon, M., Nielsen, K., Boyer, P., Liao, D., Keller, M., Deveikis, A., Nesheim, S., Lindsay, M., Lee, F., Nahmias, A., Sawyer, M., Vink, P., Farley, J., Alger, L., Abrams, E., Bamji, M., Lambert, G., Schoenbaum, E., Thomas, P., Weedon, J., Palumbo, P., Denny, T., Oleske, J., Bulterys, M., Simonds, R., Ethier-Ives, J., Rogers, M., Schluchter, M., Kutner, M., Kaplan, S., Kattan, M., Lipshultz, S., Mellins, R., Shearer, W., Sopko, G., Sloand, E., Wu, M., Kind, C., Nadal, D., Rudin, C., Siegrist, C. -A., Wyler, C. -A., Cheseaux, J. -J., Aebi, C., Gnehm, H., Schubiger, G., Klingler, J., Hunziker, U., Kuchler, H., Gianinazzi, M., Buhlmann, U., Biedermann, K., Lauper, U., Irion, O., Brunelli, A., Spoletini, G., Schreyer, A., Hosli, I., Saurenmann, E., Drack, G., Isenschmid, M., Poorbeik, M., Schupbach, J., Perrin, L., Erb, P., Joller, H., Kovacs, A., Stek, A., Chan, L., Khoury, M., Diaz, C., Pacheco-Acosta, E., Tuomala, R., Cooper, E., Mesthene, D., Pitt, J., Higgins, A., Moroso, G., Rich, K., Turpin, D., Cooper, N., Davenny, K., Thompson, B., Andiman, W., Simpson, J., THE INTERNATIONAL PERINATAL HIV, Group, Martinelli, Pasquale, Bulterys M.B., Fowler M.G., Hanson I.C., Lemay M., Mayaux M.J., Mofenson L., Newell M.-L., Peavy H., Peckham C., Read J.S., Rother C., Simpson B.J., Van Dyke R.B., Harris D.R., Peavy H.H., Easley K., Khammy A., Nugent R.P., Mitchell R., Owen W., Van Dyke R., Widmayer S., Bardeguez A., Hanson C., Wiznia A., Luzuriaga K., Viscarello R., Ho D., Koup R., Chen I., Krogstad P., Mullins J., Wolinsky S., Korber B., Walker B., Ammann A., Clapp S., McDonald D., Lapointe N., Boucher M., Fauvel M., Hankins C., Samson J., Newell M.L., Peckham C.S., Thorne C.N., Giaquinto C., Ruga E., De Rossi A., Truscia D., Grosch-Worner I., Schafer A., Mok J., Johnstone F., Jiminez J., de Alba C., Garcia Rodriguez M.C., Bates I., de Josee I., Hawkins F., Martinez Zapico R., Pena J.M., Gonzalez Garcia J., Arribas Lopez J.R., Asensi-Botet F., Otero M.C., Peerez-Tamarit D., Moya A., Galbis M.J., Scherpbier H., Boer K., Bohlin A.B., Lindgren S., Anzen B., Belfrage E., Lidin-Jansson G., Levy J., Barlow P., Hainaut M., Peltier A., Ferrazin A., De Maria A., Gotta C., Mur A., Vinolas M., Paya A., Loepez-Vilchez M.A., Coll O., Fortuny C., Boguna J., Casellas Caro M., Canet Y., Pardi G., Ravizza M., Semprini E., Castagna C., Fiore S., Guerra B., Lanari M., Bianchi S., Bovicelli L., Prati E., Zanelli S., Duse M., Soresina A., Scaravelli G., Stegagno M., De Santis M., Muggiasca M.L., Vigano A., Spinillo A., Ravagni Probizer F., Bucceri A., Rancilio L., Taylor G.P., Lyall H., Penn Z., Blott M., Valerius N.H., Martinelli P., Buffolano W., Tibaldi C., Ziarati N., Semprini A., Della Torre M., Parazzini F., Dallacasa P., Bianchi U., Pachi A., Mancuso S., Villa P., Conti M., Principi N., Muggiasca M., Marchisio P., Zara C., Ravagni F., Vignali M., Rossi G., Selvaggi L., Greco P., Vimercati A., Massi G., Innocenti T., Fiscella A., Sansone M., Benedetto C., Tadrist B., Thevenieau D., Gondry J., Paulard B., Alisy C., Brault D., Tordjeman N., Mamou J., Rozan M., Colombani D., Pincemaille O., Salvetti A., Chabanier C., Hernandorena X., Leroy J., Schaal J., Balde P., Faucher P., Lachassinne E., Benoit S., Douard D., Hocke C., Barjot P., Brouard J., Delattre P., Stien L., Audibert F., Labrune P., Vial M., Mazy F., Sitbon D., Crenn-Hebert C., Floch-Tudal C., Akakpo R., Daveau C., Leblanc A., Cesbron P., Duval-Arnould M., Huraux-Rendu C., Lemerle S., Touboul C., Guerin M., Maingueneau C., Reynaud I., Rousseau T., Ercoil V., Lanza M., Denavit M., Garnier J., Lahsinat K., Pia P., Allouche C., Nardou M., Grall F., May A., Dallot M., Lhuillier P., Cecile W., Mezin R., Bech A., Lobut J., Algava G., Chalvon Dermesay A., Busuttil R., Jacquemot M., Bader-Meunier B., Fridman S., Codaccioni X., Maxingue F., Thomas D., Alain J., De Lumley L., Tabaste J., Bailly Salin P., Seaume H., Guichard A., Kebaill K., Roussouly C., Botto C., De Lanete A., Wipff P., Cravello L., De Boisse P., Leclaire M., Michel G., Crumiere C., Lefevre V., Le Lorier B., Pauly I., Robichez B., Seguy D., Delhinger M., Rideau F., Talon P., Benos P., Huret C., Nicolas J., Heller-Roussin B., Saint-Leger S., Delaporte M., Hubert C., De Sarcus B., Karoubi P., Mechinaud F., Bertcrottiere D., Bongain A., Monpoux F., De Gennes C., Devianne F., Nisand I., Rousset M., Mouchnino G., Muray J., Munzer M., Quereux C., Brossard V., Clavier B., Allemon M., Rotten D., Stephan J., Varlet M., Guyot B., Narcy P., Bardinet F., De Caunes F., Jeny R., Robin M., Raison Boulley A., Savey L., Berrebi A., Tricoire J., Borderon J., Fignon A., Guillot F., Maria B., Broyard A., Chitrit Y., Firtion G., Mandelbrot L., Lafay Pillet M., Parat S., Boissinot C., Garec N., Levine M., Ottenwalter A., Schaller F., Vilmer E., Courpotin C., Brunner C., Ciraru-Vigneron N., Hatem-Gantzer G., Fritel X., Wallet A., Bouille J., Milliez J., Bensaid Mrejen D., Dermer E., Noseda G., Bardou D., Cressaty J., Francoual C., Carlus Moncomble C., Cohen H., Blanche S., Bastion H., Benifla J., Benkhatar F., Berkane N., Hervee F., Ronzier M., Mayaux MJ., de Martino M., Tovo P.-A., Galli L., Gabiano C., Ferraris G., Garetto S., Palomba E., Riva C., Vierucci A., de Luca M., Farina S., Fundaro C., Genovese O., Mereu G., Forni G.L., Casadei A., Zuccotti G.V., Riva E., Cellini M., Baraldi C., Consolini R., Palla G., Ruggeri M., Ciccimarra F., Guarino A., Osimani P., Benaglia G., Romano A., De Mattia D., Caselli D., Boni S., Dell'Erba G., Bassanetti F., Sticca M., Timpano C., Magnani C., Salvatore C., Lipreri R., Tornaghi R., Pinzani R., Cecchi M.T., Bezzi T., Battisti L., Bresciani E., Castelli Gattinara G., Nasi C., Pellegatta A., Mazza A., Baldi F., Altobelli R., Deiana M., Colnaghi C., Tarallo L., Tondo U., Anastasio E., Chiriaco P.G., Ruggeri C., Scott G., Hutto C., O'Sullivan M., Malmsberry A., Willoughby A., Burns D., Goedert J., Landesman S., Minkoff H., Mendez H., Holman S., Rubinstein A., Durako S., Muenz L., Goodwin S., Bryson Y., Dillon M., Nielsen K., Boyer P., Liao D., Keller M., Deveikis A., Nesheim S., Lindsay M., Lee F., Nahmias A., Sawyer M., Vink P., Farley J., Alger L., Abrams E., Bamji M., Lambert G., Schoenbaum E., Thomas P., Weedon J., Palumbo P., Denny T., Oleske J., Bulterys M., Simonds R., Ethier-Ives J., Rogers M., Schluchter M., Kutner M., Kaplan S., Kattan M., Lipshultz S., Mellins R., Shearer W., Sopko G., Sloand E., Wu M., Kind C., Nadal D., Rudin C., Siegrist C.-A., Wyler C.-A., Cheseaux J.-J., Aebi C., Gnehm H., Schubiger G., Klingler J., Hunziker U., Kuchler H., Gianinazzi M., Buhlmann U., Biedermann K., Lauper U., Irion O., Brunelli A., Spoletini G., Schreyer A., Hosli I., Saurenmann E., Drack G., Isenschmid M., Poorbeik M., Schupbach J., Perrin L., Erb P., Joller H., Kovacs A., Stek A., Chan L., Khoury M., Diaz C., Pacheco-Acosta E., Tuomala R., Cooper E., Mesthene D., Pitt J., Higgins A., Moroso G., Rich K., Turpin D., Cooper N., Davenny K., Thompson B., Andiman W., and Simpson J.
- Subjects
Time Factors ,Epidemiology ,Infectious Disease Transmission ,Prevention of perinatal transmission ,Extraembryonic Membranes ,Human immunodeficiency virus (HIV) ,HIV Infections ,medicine.disease_cause ,Cohort Studies ,Pregnancy ,Risk Factors ,INFECTION ,Vertical ,Immunology and Allergy ,HIV Infection ,MOTHER-TO-CHILD ,Pregnancy Complications, Infectious ,Prospective cohort study ,prevention of perinatal transmission ,vertical transmission ,obstetrics/gynaecology ,epidemiology ,Obstetrics ,Transmission (medicine) ,Infectious ,HUMAN-IMMUNODEFICIENCY-VIRUS, MOTHER-TO-CHILD, ZIDOVUDINE PROPHYLAXIS, RISK-FACTORS, TYPE-1, PREGNANCY, INFECTION, TRIAL, PREVENTION ,Breast Feeding ,Infectious Diseases ,Meta-analysis ,HUMAN-IMMUNODEFICIENCY-VIRUS ,Vertical transmission ,Regression Analysis ,TRIAL ,Female ,Delivery ,Obstetrics gynaecology ,Human ,medicine.medical_specialty ,Time Factor ,Ruptured membranes ,Immunology ,Regression Analysi ,NO ,ZIDOVUDINE PROPHYLAXIS ,Extraembryonic Membrane ,medicine ,Humans ,TYPE-1 ,business.industry ,Risk Factor ,Infant, Newborn ,Infant ,Obstetric ,Delivery, Obstetric ,Newborn ,PREVENTION ,Infectious Disease Transmission, Vertical ,Pregnancy Complications ,Obstetrics/gynaecology ,RISK-FACTORS ,Cohort Studie ,business - Abstract
Objective: To test the a priori hypothesis that longer duration of ruptured membranes is associated with increased risk of vertical transmission of HIV. Design: The relationship between duration of ruptured membranes and vertical transmission of HIV was evaluated in an individual patient data meta-analysis. Methods: Eligible studies were prospective cohort studies including at least 100 mother-child pairs, from regions where HIV-infected women are counselled not to breastfeed. Analyses were restricted to vaginal deliveries and non-elective Cesarean sections; elective Cesarean section deliveries (those performed before onset of labour and before rupture of membranes) were excluded. Results: The primary analysis included 4721 deliveries with duration of ruptured membranes ≤ 24 h. After adjusting for other factors known to be associated with vertical transmission using logistic regression analysis to assess the strength of the relationship, the risk of vertical HIV transmission increased approximately 2% with an increase of 1 h in the duration of ruptured membranes [adjusted odds ratio, 1.02; 95% confidence interval, 1.01-1.04; for each 1 h increment]. There were no significant interactions of duration of ruptured membranes with study cohort or with any of the covariates, except maternal AIDS. Among women diagnosed with AIDS, the estimated probability of transmission increased from 8% to 31% with duration of ruptured membranes of 2 h and 24 h respectively (P < 0.01). Conclusions: These results support the importance of duration of ruptured membranes as a risk factor for vertical transmission of HIV and suggest that a diagnosis of AIDS in the mother at the time of delivery may potentiate the effect of duration of ruptured membranes. © 2001 Lippincott Williams & Wilkins.
- Published
- 2001
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5. The Mode of Delivery and the Risk of Vertical Transmission of Human Immunodeficiency Virus Type 1 — A Meta-Analysis of 15 Prospective Cohort Studies
- Author
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Andiman, W., Boucher, M., Burns, D., Bryson, Y., Farley, J., Fowler, H., Gabiano, C., Galli, L., Hutto, C., Kind, C., Korber, B., Kovacs, A., Krogstad, P., Landesman, S., Lapointe, N., Lemay, M., Lew, J., Mandelbrot, L., Mayaux, Mj, Mellins, R., Minkoff, H., Mofenson, L., Nielsen, K., Newell, Ml, Pardi, G., Peavy, H., Peckham, C., Read, J., Rother, C., Rudin, C., Scott, G., Semprini, A., Shearer, W., Simonds, R., Simpson, B., Stek, A., Tovo, Pa, Tuomala, R., Dyke, R., Weedon, J., Martino, M., Lindsay, M., Belair, S., Chan, L., Harris, D., Kalish, L., Muenz, L., Nugent, R., Schluchter, M., Durako, S., Goodwin, S., Mitchell, R., Nourjah, P., Owen, W., Widmayer, S., Bardeguez, A., Hanson, C., Wiznia, A., Luzuriaga, K., Viscarello, R., Ho, D., Koup, R., Chen, I., Mullins, J., Wolinsky, S., Walker, B., Ammann, A., Clapp, S., Mcdonald, D., Fauvel, M., Hankins, C., Samson, J., Bailey, A., Giaquinto, C., Ruga, E., Rossi, A., Truscia, D., Grosch-Worner, I., Schafer, A., Mok, J., Johnstone, F., Jiminez, J., Alba, C., Garcia-Rodriguez, M., Bates, I., Jose, I., Hawkins, F., Zapico, Rm, Asensi-Botet, F., Otero, M., Perez-Tamarit, D., Moya, A., Galbis, M., Scherpbier, H., Boer, K., Bohlin, A., Lindgren, S., Ehrnst, A., Anzen, B., Belfrage, E., Levy, J., Alimenti, A., Barlow, P., Ferrazin, A., Maria, A., Gotta, C., Maritati, V., Mur, A., Rovira, M., Paya, A., Coll, O., Fortuny, C., Boguna, J., Caro, Mc, Canet, Y., Ravizza, M., Castagna, C., Fiore, S., Guerra, B., Lanari, M., Bianchi, S., Bovicelli, L., Prati, E., Duse, M., Soresina, A., Scaravelli, G., Santis, M., Muggiasca, M., Vigano, A., Marchisio, P., Iasci, A., Spinillo, A., Bucceri, A., Grossi, E., Rancilio, L., Della Torre, M., Dallacasa, P., Pachi, A., Principi, N., Zara, C., Vignali, M., Rossi, G., Selvaggi, L., Greco, P., Vimercati, A., Massi, G., Innocenti, T., Fiscella, A., Sansone, M., Benedetto, C., Tibaldi, C., Ziarati, N., Tadrist, B., Thevenicau, D., Gondry, J., Paulard, B., Alisy, C., Brault, D., Tordjeman, P., Mamou, J., Rozan, M., Colombani, D., Pincemaille, O., Salvetti, A., Chabanier, C., Hernandorena, X., Leroy, J., Schaal, J., Balde, P., Faucher, P., Lachassinne, E., Benoit, S., Douard, D., Hocke, C., Barjot, P., Brouard, J., Delattre, P., Stien, L., Audibert, F., Labrune, P., Vial, M., Mazy, F., Sitbon, D., Crenn-Hebert, C., Floch-Tudal, C., Akakpo, R., Daveau, C., Leblanc, A., Cesbron, P., Duval-Arnould, H., Huraux-Rendu, C., Lemerle, S., Touboul, C., Guerin, M., Maingueneau, C., Reynaud, I., Rousseau, T., Ercoil, V., Lanza, M., Denavit, M., Garnier, J., Lahsinat, K., Pia, R., Allouche, C., Nardou, M., Grall, F., May, A., Dallot, M., Lhuillier, P., Cecile, W., Mezin, R., Bech, A., Lobut, J., Algava, G., Dermesay, Ac, Busuttil, R., Jacquemot, M., Bader-Meunier, B., Fridman, S., Codaccioni, X., Maxingue, F., Thomas, D., Alain, J., Lumley, L., Tabaste, J., Salin, Pb, Seaume, H., Guichard, A., Kebaili, K., Roussouly, C., Botto, C., Lanete, A., Wipff, P., Cravello, L., Boisse, P., Leclaire, M., Michel, G., Crumiere, C., Lefevre, V., Le Lorier, B., Pauly, I., Robichez, B., Seguy, D., Dehlinger, M., Rideau, F., Talon, P., Benos, P., Huret, C., Nicolas, J., Heller-Roussin, B., Saint-Leger, S., Delaporte, M., Hubert, C., Sarcus, B., Karoubi, P., Mechinaud, F., Bertcrottiere, D., Bongain, A., Monpoux, F., Gennes, C., Devianne, F., Nisand, I., Rousset, M., Mouchnino, G., Muray, J., Munzer, M., Quereux, C., Brossard, V., Clavier, B., Allemon, M., Rotten, D., Stephan, J., Varlet, M., Guyot, B., Narey, P., Bardinet, F., Caunes, F., Jeny, R., Robin, M., Bouley, Ar, Savey, L., Berrebi, A., Tricoire, J., Borderon, J., Fignon, A., Guillot, F., Maria, B., Broyard, A., Chitrit, Y., Firtion, G., Pillet, Ml, Parat, S., Boissinot, C., Garec, N., Levine, M., Ottenwalter, A., Schaller, F., Vilmer, B., Courpotin, C., Brunner, C., Ciraru-Vigneron, N., Hatem-Gantzer, G., Xavier FRITEL, Wallet, A., Bouille, J., Milliez, J., Mrejen, Db, Dermer, E., Noseda, G., Bardou, D., Cressaty, J., Francoual, C., Moncomble, Cc, Cohen, H., Blanche, S., Bastion, H., Benifla, J., Benkhatar, F., Berkane, N., Herve, F., Ronzier, M., Ferraris, G., Rancillo, L., Tulisso, S., Scolfaro, C., Riva, C., Vierucci, A., Luca, M., Farina, S., Fundaro, C., Genovese, O., Mercu, G., Forni, G., Stegagno, M., Falconieri, P., Zuccotti, G., Riva, E., Cellini, M., Baraldi, C., Consolini, R., Palla, G., Ruggeri, M., Pignata, C., Guarino, A., Osimani, P., Metri, A., Antonellini, A., Benaglia, G., Romano, A., Mattia, D., Caselli, D., Boni, S., Erba, G., Bassanetti, F., Sticca, M., Timpano, C., Magnani, C., Salvatore, C., Gambaretto, G., Lipreri, R., Tornaghi, R., Pinzani, R., Cecchi, M., Bezzi, T., Battisti, L., Bresciani, E., Gattinara, G., Berrino, R., Pellegatta, A., Mazza, A., Baldi, F., Micheletti, E., Altobelli, R., Deiana, M., Colnaghi, C., Tarallo, L., Tondo, U., Anastasio, E., Chiriaco, P., Contardi, I., Ruggeri, C., Ibba, P., O Sullivan, M., Malmsberry, A., Willoughby, A., Goedert, J., Mendez, H., Holman, S., Rubinstein, A., Nesheim, S., Clark, S., Lee, F., Nahmias, A., Sawyer, M., Vink, P., Alger, L., Abrams, E., Bamji, M., Lambert, G., Schoenbaum, E., Thea, D., Thomas, P., Palumbo, P., Denny, T., Oleske, J., Orloff, S., Ethier-Ives, J., Rogers, M., Kutner, M., Kaplan, S., Kattan, M., Lipshultz, S., Sopko, G., Sloand, E., Wu, M., Nadal, D., Siegrist, Ca, Wyler, Ca, Cheseaux, Jj, Aebi, C., Gnehm, H., Schubiger, G., Klingler, J., Hunziker, U., Kuchler, H., Gianinazzi, M., Buhlmann, U., Biedermann, K., Lauper, U., Irion, O., Brunelli, A., Spoletini, G., Schreyer, A., Hosli, I., Saurenmann, E., Drack, G., Isenschmid, M., Poorbeik, M., Schupbach, J., Perrin, L., Erb, P., Joller, H., Dillon, M., Nielsen, R., Boyer, P., Liao, D., Keller, M., Deveikis, A., Khoury, M., Diaz, C., Pacheco-Acosta, E., Cooper, E., Mesthene, D., Pitt, J., Higgins, A., Moroso, G., Rich, K., Turpin, D., Cooper, N., Fowler, M., Smeriglio, V., Mckinlay, S., and Ellis, S.
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Male ,medicine.medical_specialty ,Multivariate analysis ,Anti-HIV Agents ,Birth weight ,HIV Infections ,Cohort Studies ,Pregnancy ,Risk Factors ,medicine ,Birth Weight ,Humans ,Rupture of membranes ,Pregnancy Complications, Infectious ,Prospective cohort study ,Cesarean Section ,Obstetrics ,business.industry ,Infant, Newborn ,General Medicine ,Odds ratio ,Delivery, Obstetric ,medicine.disease ,Infectious Disease Transmission, Vertical ,Confidence interval ,Logistic Models ,Multivariate Analysis ,Immunology ,HIV-1 ,Female ,business ,Zidovudine ,Cohort study - Abstract
Background To evaluate the relation between elective cesarean section and vertical transmission of human immunodeficiency virus type 1 (HIV-1), we performed a meta-analysis using data on individual patients from 15 prospective cohort studies. Methods North American and European studies of at least 100 mother-child pairs were included in the meta-analysis. Uniform definitions of modes of delivery were used. Elective cesarean sections were defined as those performed before onset of labor and rupture of membranes. Multivariate logistic-regression analysis was used to adjust for other factors known to be associated with vertical transmission. Results The primary analysis included data on 8533 mother-child pairs. After adjustment for receipt of antiretroviral therapy, maternal stage of disease, and infant birth weight, the likelihood of vertical transmission of HIV-1 was decreased by approximately 50 percent with elective cesarean section, as compared with other modes of delivery (adjusted odds ratio, 0.43; 95 percent confidence interval, 0.33 to 0.56). The results were similar when the study population was limited to those with rupture of membranes shortly before delivery. The likelihood of transmission was reduced by approximately 87 percent with both elective cesarean section and receipt of antiretroviral therapy during the prenatal, intrapartum, and neonatal periods, as compared with other modes of delivery and the absence of therapy (adjusted odds ratio, 0.13; 95 percent confidence interval, 0.09 to 0.19). Among mother-child pairs receiving antiretroviral therapy during the prenatal, intrapartum, and neonatal periods, rates of vertical transmission were 2.0 percent among the 196 mothers who underwent elective cesarean section and 7.3 percent among the 1255 mothers with other modes of delivery. Conclusions The results of this meta-analysis suggest that elective cesarean section reduces the risk of transmission of HIV-1 from mother to child independently of the effects of treatment with zidovudine.
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- 1999
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6. Determinants of mother-to-infant human immunodeficiency virus 1 transmission before and after the introduction of zidovudine prophylaxis
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de Martino, M, Galli, L, Tovo, PA, Gabiano, C, Pezzotti, P, Wagner, TM, Rezza, G, Osimani, P, De Mattia, D, Di Bari, C, Ruggeri, M, Baldi, F, Ciccia, M, Lanari, M, Masi, M, Venturi, V, Battisti, L, Duse, M, Chiriaco, PG, Cavallini, R, Dessi, C, Pintor, C, Anastasio, E, SABATINO, GIOVANNI, Sticca, M, Pomero, G, Bezzi, T, Chiappini, E, De Luca, M, Gervaso, P, Cecchi, MT, Bassetti, D, Gotta, C, Rosso, R, Timitilli, A, Tondo, U, Mussini, P, Bricalli, D, BUCCERI, ANDREA, Ferraris, G, Giovannini, M, Mosca, F, Lipreri, R, Guarino, A, Plebani, A, Riva, E, RIVA, SILVIA, Vigano, A, Zuccotti, GV, Cellini, M, Buffolano, W, Tarallo, L, D'Elia, R, Giaquinto, C, Rampon, O, Dalle Nogare, ER, Romano, A, Caselli, D, Maccabruni, A, Consolini, R, Benaglia, G, Magnani, C, Anzidei, G, Pistilli, AMC, Gattinara, GC, Catania, S, Facente, C, Falconieri, P, Fundaro, C, Genovese, O, Rendeli, C, Bionda, S, Cristiano, L, Garetto, S, Riva, C, PALOMBA, ERIKA, Portelli, V, Mazza, A, SALVATORE, CLAUDIO, Pellegatta, A, Molesini, M, de Martino, M, Galli, L, Tovo, PA, Gabiano, C, Pezzotti, P, Wagner, TM, Rezza, G, Osimani, P, De Mattia, D, Di Bari, C, Ruggeri, M, Baldi, F, Ciccia, M, Lanari, M, Masi, M, Venturi, V, Battisti, L, Duse, M, Chiriaco, PG, Cavallini, R, Dessi, C, Pintor, C, Anastasio, E, Sabatino, G, Sticca, M, Pomero, G, Bezzi, T, Chiappini, E, De Luca, M, Gervaso, P, Cecchi, MT, Bassetti, D, Gotta, C, Rosso, R, Timitilli, A, Tondo, U, Mussini, P, Bricalli, D, Bucceri, A, Ferraris, G, Giovannini, M, Mosca, F, Lipreri, R, Guarino, A, Plebani, A, Riva, E, Riva, S, Vigano, A, Zuccotti, GV, Cellini, M, Buffolano, W, Tarallo, L, D'Elia, R, Giaquinto, C, Rampon, O, Dalle Nogare, ER, Romano, A, Caselli, D, Maccabruni, A, Consolini, R, Benaglia, G, Magnani, C, Anzidei, G, Pistilli, AMC, Gattinara, GC, Catania, S, Facente, C, Falconieri, P, Fundaro, C, Genovese, O, Rendeli, C, Bionda, S, Cristiano, L, Garetto, S, Riva, C, Palomba, E, Portelli, V, Mazza, A, Salvatore, C, Pellegatta, A, and Molesini, M
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immunodeficiency virus 1 - Abstract
Background: Randomized controlled trials have demonstrated that zidovudine therapy decreases the mother-to-infant transmission of human immunodeficiency virus 1 (HIV-1). Data from large observational studies may provide further important findings on the effectiveness at the population level of combined treatments in decreasing transmission.Objective: To evaluate time trends in prophylactic interventions and the determinants of transmission both before and after the introduction of antiretroviral prophylaxis, and in treated and untreated mother-infant pairs after 1995.Design and Setting: Analysis of prospective data on 3770 children born to HIV-1-infected women between 1985 and 1999 and reported to the Italian Register for HIV Infection in Children.Main Outcome Measures: Logistic regression random effects models were used to estimate crude and adjusted odds ratios for several factors potentially influencing vertical transmission for 2periods-1985 through 1995 (January 1, 1985, through December 31,1995) and 1996 through 1999 (January 1, 1996, through December 31, 1999), and between treated and untreated children after 1995.Results: The transmission rate was 15.5% in the 19851995 period and 5.8% in the 1996-1999 period. By 1999, prophylactic interventions had greatly increased. Antiretroviral treatment (ART) usage was 89.9%, (55.1% combination ART) and the elective cesarean delivery rate was 81.3%. In multivariate analysis, only elective cesarean delivery was associated with a lower risk of mother-to-infant transmission before 1995. After 1995, nonbreastfeeding and receipt of ART were protective whereas elective cesarean delivery was not significantly protective in multivariate analysis. Transmission risk was reduced by 76% with an incomplete zidovudine regimen, 88% with a complete regimen, and 93% when the mother received combination ART. In the 1996-1999 period, the transmission rate for nonbreastfeeding mother-infant pairs was 8.6% with elective cesarean delivery, 4.4% with any ART, and 2.4% with these interventions combined.Conclusion: Prophylactic interventions, and in particular ART, reduced perinatal HIV-1 transmission at a population level in Italy.
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- 2004
7. Puberty in perinatal HIV-1 infection: a multicentre longitudinal study of 212 children
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de Martino M, Tovo PA, Galli L, Gabiano C, Chiarelli F, Zappa M, Gattinara GC, Bassetti D, Giacomet V, Chiappini E, Duse M, Garetto S, Caselli D, Italian Register for HIV infection in Children ( Catania S, FundaroÁ C, Cellini M, Lipreri R, Zuccotti GV, Cecchi MT, Mazza A, Masi M, Consolini R, Bezzi MT, Benaglia G, Ganau A., GUARINO, ALFREDO, de Martino, M, Tovo, Pa, Galli, L, Gabiano, C, Chiarelli, F, Zappa, M, Gattinara, Gc, Bassetti, D, Giacomet, V, Chiappini, E, Duse, M, Garetto, S, Caselli, D, Italian Register for HIV infection in Children, ( Catania S, Fundaroá, C, Cellini, M, Lipreri, R, Zuccotti, Gv, Cecchi, Mt, Guarino, Alfredo, Mazza, A, Masi, M, Consolini, R, Bezzi, Mt, Benaglia, G, and Ganau, A.
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Male ,Percentile ,Pediatrics ,medicine.medical_specialty ,Longitudinal study ,adolescence ,HIV-1 ,perinatal infection ,puberty ,Tanner stage ,Adolescent ,Anti-HIV Agents ,Immunology ,HIV Infections ,Age Distribution ,Acquired immunodeficiency syndrome (AIDS) ,Immunopathology ,medicine ,Immunology and Allergy ,Humans ,Longitudinal Studies ,Child ,Rank correlation ,business.industry ,Puberty ,Infant, Newborn ,medicine.disease ,Confidence interval ,Log-rank test ,Fetal Diseases ,Infectious Diseases ,El Niño ,Female ,business - Abstract
OBJECTIVE: To define age at entry into Tanner stages in children with perinatal HIV-1 infection. DESIGN: Multicentre longitudinal study including 212 perinatally HIV-1-infected children (107 girls and 105 boys) followed-up during puberty (from 8 and 9 years onwards in girls and boys, respectively). Healthy children (843 girls and 821 boys) provided reference percentiles. P2 or B2 stages in girls and P2 or G2 stages in boys defined onset of puberty. METHODS: The cumulative probability [95% confidence limit (CI)] of entry into each stage at different ages was estimated by the Kaplan-Meier product-limit method; differences were evaluated by log rank test. Relationships were tested using the Spearman's rank correlation coefficient. RESULTS: Ages of girls [years (95%CI)] at P2 [12.9 (12.6-13.2)], P3 [13.4 (13.0-13.8)], P4 [14.6 (14.0-15.2)], B2 [12.7 (12.2-13.2)], B3 [13.3 (12.8-14.0)] and B4 [14.6 (14.0-15.2)] stages were > 97th percentile (> or = 21 month delay) of controls. Ages of boys [years (95%CI)] at P2 [12.6 (12.1-13.1)], P3 [13.9 (13.4-14.4)], P4 [14.9 (14.2-15.6)], G2 [12.1 (11.5-12.7)], G3 [13.6 (13.1-14.1)] and G4 [14.9 (14.1-15.7)] stages were at the 75-97th percentiles (< or = 15 month delay). Age at onset of puberty was not related to clinical and immunological condition, antiretroviral treatment, weigh for height and age at onset of severe disease or immune suppression. CONCLUSION: Perinatal HIV-1 infection interferes with sexual maturation. The mechanisms by which this occurs should be elucidated and intervention strategies designed. Intervention could save much psychological distress, since associated linear growth failure can exacerbate adolescents' feelings of being different and unwell.
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- 2001
8. Reduction in mortality with availability of antiretroviral therapy for children with perinatal HIV-1 infection. Italian Register for HIV Infection in Children and the Italian National AIDS Registry
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de Martino M, Tovo PA, Balducci M, Galli L, Gabiano C, Rezza G, Pezzotti P, Osimani P, Di Bari C, Larovere D, Ruggeri m, Masi M, SpecchiaM, Battisti L, Duse M, Crispino P, Carrara P, Pintor C, Dedoni M, Dessì C, Loriano D, Anastasio E, Bezzi T, De Luca M, Farina S, Vierucci A, Bassetti D, Pontali E, Boni S, Marazzi M. G, Tasso L, Giovanettoni C, Salvini F, Pinzani R, Marchisio P, Viganò A, Tornaghi R, Zuccotti G. V, Riva E, Giovannini M, Lipreri R, Conio S, Ferraris G, Cellini M, Baraldi C, Tarallo L, Giaquinto C, Ruga E, Rampon O, Dalle Nogare E. R, Sanfilippo A, Romano A, Benaglia G, Dodi I, Caselli D, maccabruni A, Pacati I, Consolini R, Palla G, Cecchi M. T, Vecchi C, Anzidei G, Cerilli S, Chiodi R, Castelli Gattianra G, Krzysztofiak A, Bernardi S, Fundarò C, Genovese O, Colafati G. S, Catania A, Ajassa C, Mazza A, Garetto S, Riva C, Scolfaro C., GUARINO, ALFREDO, BERNI CANANI, ROBERTO, de Martino, M, Tovo, Pa, Balducci, M, Galli, L, Gabiano, C, Rezza, G, Pezzotti, P, Osimani, P, Di Bari, C, Larovere, D, Ruggeri, M, Masi, M, Specchiam, Battisti, L, Duse, M, Crispino, P, Carrara, P, Pintor, C, Dedoni, M, Dessì, C, Loriano, D, Anastasio, E, Bezzi, T, De Luca, M, Farina, S, Vierucci, A, Bassetti, D, Pontali, E, Boni, S, Marazzi, M. G., Tasso, L, Giovanettoni, C, Salvini, F, Pinzani, R, Marchisio, P, Viganò, A, Tornaghi, R, Zuccotti, G. V., Riva, E, Giovannini, M, Lipreri, R, Conio, S, Ferraris, G, Cellini, M, Baraldi, C, Guarino, Alfredo, BERNI CANANI, Roberto, Tarallo, L, Giaquinto, C, Ruga, E, Rampon, O, Dalle Nogare, E. R., Sanfilippo, A, Romano, A, Benaglia, G, Dodi, I, Caselli, D, Maccabruni, A, Pacati, I, Consolini, R, Palla, G, Cecchi, M. T., Vecchi, C, Anzidei, G, Cerilli, S, Chiodi, R, Castelli Gattianra, G, Krzysztofiak, A, Bernardi, S, Fundarò, C, Genovese, O, Colafati, G. S., Catania, A, Ajassa, C, Mazza, A, Garetto, S, Riva, C, and Scolfaro, C.
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CONTEXT: Since the introduction of combined antiretroviral therapy, mortality rates in adults with human immunodeficiency virus type 1 (HIV-1) infection have decreased. However, little information is available outside the setting of controlled trials on survival of perinatally HIV-infected children treated with antiretroviral therapy. OBJECTIVE: To assess effect of availability of antiretroviral therapy on decreasing mortality in perinatally HIV-infected children. DESIGN: Population-based, multicenter longitudinal study involving data collected by the Italian Register for HIV Infection in Children. SETTING: A network of 106 pediatric clinical centers. SUBJECTS: A total of 1142 children born between November 1980 and December 1997 with perinatally acquired HIV infection with a median follow-up of 5.9 years. MAIN OUTCOME MEASURE: Time to HIV-related death calculated for birth cohort and calendar period and grouped by distribution of predominant type of antiretroviral therapy administered over time. RESULTS: Survival was longer in the 1996-1997 birth cohort (crude relative hazard [RH] of death, 0.39; 95% confidence interval [CI], 0.15-0.96) and 1996-1998 calendar period (crude RH of death, 0.65; 95% CI, 0.45-0.95) than in birth cohort and calendar period 1980-1995, but not when adjusted for maternal antiretroviral treatment during pregnancy and clinical condition at time of delivery, gestational age, and birth weight (adjusted RH of death, 0.55; 95% CI, 0.20-1.50, for birth cohort; and adjusted RH of death, 0.71, 95% CI, 0.43-1.16, for calendar period). In a multivariate model with 1980-1995 as comparison, the 1996-1997 birth cohort had an RH of 0.57 (95% CI, 0.22-1.47; P=.27) but RH for calendar period 1996-1998 was 0.63 (95% CI, 0.47-0.85; P
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- 2000
9. Correction of neuropathology and behavioural deficits in ASA-/- deficient mice after lentiviral-mediated gene transfer into the brain
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Consiglio, A., Martino, Sabata, Quattrini, A., Dolcetta, D., Bensadoun, J. C., Trojani, A., Benaglia, G., Marchesini, S., Cestari, V., and Naldini, L.
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- 2010
10. HIV-1 transmission through breast-milk
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De Martino, M., Tovo, P. A., Tozzi, A. E., Pezzotti, P., Galli, L., Liviadotti, S., Caselli, D., Marchisio, P., Giaquinto, G., Fioredda, F., Plebani, A., Gabiano, C., Zuccotti, V. G., Conte, A., Rizzi, M., Mazzoni, P. L., Ibba, P., Ferrarris, G., Benaglia, G., Stegagno, M., Masi, M., Dallacasa, P., Duse, Marzia, Rossi, G., Sciotto, A., Barbanera, M., De Mattia, D., Zaniboni, M., Bezzi, T., Campelli, A., Ciccimarra, F., Bassanetti, F., Consolini, R., Mazza, A., Tarallo, L., Altobelli, R., Castaldo, A., and Fundarò, C.
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medicine.medical_specialty ,Multivariate analysis ,business.industry ,Immunology ,Gestational age ,Odds ratio ,Logistic regression ,medicine.disease ,Confidence interval ,Surgery ,Infectious Diseases ,Acquired immunodeficiency syndrome (AIDS) ,Immunology and Allergy ,Medicine ,Risk factor ,business ,Breast feeding ,Demography - Abstract
Objectives To estimate the risk of HIV-1 transmission through breast-milk in children born to infected mothers, and to determine the relationship between duration of breast-feeding and risk. Design and methods The study population included 168 breast-fed and 793 bottle-fed children born to seropositive mothers. All subjects were enrolled and followed-up in the Italian Register for HIV Infection in Children; HIV sero-status was defined in all children. Multivariate analysis was performed using a logistic regression model. Independent variables included biological factors (duration of breast-feeding, gestational age, clinical condition of mother at delivery, mode of delivery, birth-weight and sex). Year of birth and age when HIV infection was diagnosed were also considered in the analysis attempting to control for possible selection biases. Results Breast-feeding increased the risk of HIV-1 transmission. The estimated adjusted odds ratio for 1 day of breast- versus bottle-feeding was 1.19 (95% confidence interval, 1.10-1.28). The infection odds ratio of breast- versus bottle-feeding increased with the natural logarithm of the duration of practice. Conclusions These results are the first to provide an appraisal of the additional risk of HIV-1 transmission associated with a seropositive mother breast-feeding her child. Biological significance of this route of transmission was supported by demonstration of a relationship between duration of breast-feeding and risk of HIV-1 transmission.
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- 1992
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11. The mode of delivery and the risk of vertical transmission of human immunodeficiency virus type 1--a meta-analysis of 15 prospective cohort studies. The International Perinatal HIV Group
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Andiman W., Boucher M., Burns D., Bryson Y., Farley J., Fowler H., Gabiano C., Galli L., Hutto C., Kind C., Korber B., Kovacs A., Krogstad P., Landesman S., Lapointe N., Lemay M., Lew J., Mandelbrot L., Mayaux M.J., Mellins R., Minkoff H., Mofenson L., Nielsen K., Newell M.L., Pardi G., Peavy H., Peckham C., Read J., Rother C., Rudin C., Scott G., Semprini A., Shearer W., Simonds R., Simpson B., Stek A., Tovo P.A., Tuomala R., Van Dyke R., Weedon J., de Martino M., Lindsay M., Belair S., Chan L., Harris D., Kalish L., Muenz L., Nugent R., Schluchter M., Durako S., Goodwin S., Mitchell R., Nourjah P., Owen W., Widmayer S., Bardeguez A., Hanson C., Wiznia A., Luzuriaga K., Viscarello R., Ho D., Koup R., Chen I., Mullins J., Wolinsky S., Walker B., Ammann A., Clapp S., McDonald D., Fauvel M., Hankins C., Samson J., Bailey A., Giaquinto C., Ruga E., De Rossi A., Truscia D., Grosch Worner I., Schafer A., Mok J., Johnstone F., Jiminez J., de Alba C., Garcia Rodriguez M., Bates I., de Jose I., Hawkins F., Zapico R.M., Asensi Botet F., Otero M., Perez Tamarit D., Moya A., Galbis M., Scherpbier H., Boer K., Bohlin A., Lindgren S., Ehrnst A., Anzen B., Belfrage E., Levy J., Alimenti A., Barlow P., Ferrazin A., De Maria A., Gotta C., Maritati V., Mur A., Rovira M., Paya A., Coll O., Fortuny C., Boguna J., Caro M.C., Canet Y., Ravizza M., Castagna C., Fiore S., Guerra B., Lanari M., Bianchi S., Bovicelli L., Prati E., Duse M., Soresina A., Scaravelli G., De Santis M., Muggiasca M., Vigano A., Marchisio P., Iasci A., Spinillo A., Bucceri A., Grossi E., Rancilio L., Della Torre M., Dallacasa P., Pachi A., Principi N., Zara C., Vignali M., Rossi G., Selvaggi L., Greco P., Vimercati A., Massi G., Innocenti T., Fiscella A., Sansone M., Benedetto C., Tibaldi C., Ziarati N., Tadrist B., Thevenicau D., Gondry J., Paulard B., Alisy C., Brault D., Tordjeman P., Mamou J., Rozan M., Colombani D., Pincemaille O., Salvetti A., Chabanier C., Hernandorena X., Leroy J., Schaal J., Balde P., Faucher P., Lachassinne E., Benoit S., Douard D., Hocke C., Barjot P., Brouard J, Delattre P, Stien L, Audibert F, Labrune P, Vial M, Mazy F, Sitbon D, Crenn Hebert C, Floch Tudal C, Akakpo R, Daveau C, Leblanc A, Cesbron P, Duval Arnould H, Huraux Rendu C, Lemerle S, Touboul C, Guerin M, Maingueneau C, Reynaud I, Rousseau T, Ercoil V, Lanza M, Denavit M, Garnier J, Lahsinat K, Pia R, Allouche C, Nardou M, Grall F, May A, Dallot M, Lhuillier P, Cecile W, Mezin R, Balde P, Bech A, Lobut J, Algava G, Dermesay AC, Busuttil R, Jacquemot M, Bader Meunier B, Fridman S, Codaccioni X, Maxingue F, Thomas D, Alain J, De Lumley L, Tabaste J, Salin PB, Seaume H, Guichard A, Kebaili K, Roussouly C, Botto C, De Lanete A, Wipff P, Cravello L, De Boisse P, Leclaire M, Michel G, Crumiere C, Lefevre V, Le Lorier B, Pauly I, Robichez B, Seguy D, Dehlinger M, Rideau F, Talon P, Benos P, Huret C, Nicolas J, Heller Roussin B, Saint Leger S, Delaporte M, Hubert C, De Sarcus B, Karoubi P, Mechinaud F, Bertcrottiere D, Bongain A, Monpoux F, De Gennes C, Devianne F, Nisand I, Rousset M, Mouchnino G, Muray J, Munzer M, Quereux C, Brossard V, Clavier B, Allemon M, Rotten D, Stephan J, Varlet M, Guyot B, Narey P, Bardinet F, De Caunes F, Jeny R, Robin M, Bouley AR, Savey L, Berrebi A, Tricoire J, Borderon J, Fignon A, Guillot F, Maria B, Broyard A, Chitrit Y, Firtion G, Mandelbrot L, Pillet ML, Parat S, Boissinot C, Garec N, Levine M, Ottenwalter A, Schaller F, Vilmer B, Courpotin C, Brunner C, Ciraru Vigneron N, Hatem Gantzer G, Fritel X, Wallet A, Bouille J, Milliez J, Mrejen DB, Dermer E, Noseda G, Bardou D, Cressaty J, Francoual C, Moncomble CC, Cohen H, Blanche S, Bastion H, Benifla J, Benkhatar F, Berkane N, Herve F, Ronzier M, Mayaux MJ, de Martino M, Tovo PA, Galli L, Gabiano C, Ferraris G, Rancillo L, Bucceri A, Tulisso S, Scolfaro C, Riva C, Vierucci A, de Luca M, Farina S, Fundaro C, Genovese O, Mercu G, Forni G, Stegagno M, Falconieri P, Zuccotti G, Riva E, Cellini M, Baraldi C, Consolini R, Palla G, Ruggeri M, Osimani P, Metri A, Antonellini A, Benaglia G, Romano A, Dallacasa P, De Mattia D, Caselli D, Boni S, Dell'Erba G, Bassanetti F, Sticca M, Timpano C, Magnani C, Salvatore C, Gambaretto G, Lipreri R, Tornaghi R, Pinzani R, Cecchi M, Bezzi T, Battisti L, Bresciani E, Gattinara G, Berrino R, Pellegatta A, Mazza A, Baldi F, Micheletti E, Ruga E, Altobelli R, Deiana M, Colnaghi C, Tarallo L, Tondo U, Anastasio E, Duse M, Chiriaco P, Contardi I, Ruggeri C, Ibba P, Scott G, Hutto C, O'Sullivan M, Malmsberry A, Willoughby A, Burns D, Goedert J, Landesman S, Minkoff H, Mendez H, Holman S, Rubinstein A, Durako S, Muenz L, Goodwin S, Nesheim S, Lindsay M, Clark S, Lee F, Nahmias A, Sawyer M, Vink P, Farley J, Alger L, Abrams E, Bamji M, Lambert G, Schoenbaum E, Thea D, Thomas P, Weedon J, Palumbo P, Bardeguez A, Denny T, Oleske J, Simonds R, Orloff S, Ethier Ives J, Rogers M, Schluchter M, Kutner M, Kaplan S, Kattan M, Lipshultz S, Mellins R, Shearer W, Peavy H, Sopko G, Sloand E, Wu M, Kind C, Nadal D, Rudin C, Siegrist CA, Wyler CA, Cheseaux JJ, Aebi C, Gnehm H, Schubiger G, Klingler J, Hunziker U, Kuchler H, Gianinazzi M, Buhlmann U, Biedermann K, Lauper U, Irion O, Brunelli A, Spoletini G, Schreyer A, Hosli I, Saurenmann E, Drack G, Isenschmid M, Poorbeik M, Schupbach J, Perrin L, Erb P, Joller H, Bryson Y, Dillon M, Nielsen R, Boyer P, Liao D, Keller M, Deveikis A, Kovacs A, Stek A, Chan L, Rother C, Khoury M, Diaz C, Pacheco Acosta E, Tuomala R, Cooper E, Mesthene D, Pitt J, Higgins A, Moroso G, Rich K, Turpin D, Cooper N, Fowler M, Nugent R, Smeriglio V, McKinlay S, Kalish L, Ellis S, Andiman W, PIGNATA, CLAUDIO, GUARINO, ALFREDO, Andiman, W., Boucher, M., Burns, D., Bryson, Y., Farley, J., Fowler, H., Gabiano, C., Galli, L., Hutto, C., Kind, C., Korber, B., Kovacs, A., Krogstad, P., Landesman, S., Lapointe, N., Lemay, M., Lew, J., Mandelbrot, L., Mayaux, M. J., Mellins, R., Minkoff, H., Mofenson, L., Nielsen, K., Newell, M. L., Pardi, G., Peavy, H., Peckham, C., Read, J., Rother, C., Rudin, C., Scott, G., Semprini, A., Shearer, W., Simonds, R., Simpson, B., Stek, A., Tovo, P. A., Tuomala, R., Van Dyke, R., Weedon, J., de Martino, M., Lindsay, M., Belair, S., Chan, L., Harris, D., Kalish, L., Muenz, L., Nugent, R., Schluchter, M., Durako, S., Goodwin, S., Mitchell, R., Nourjah, P., Owen, W., Widmayer, S., Bardeguez, A., Hanson, C., Wiznia, A., Luzuriaga, K., Viscarello, R., Ho, D., Koup, R., Chen, I., Mullins, J., Wolinsky, S., Walker, B., Ammann, A., Clapp, S., Mcdonald, D., Fauvel, M., Hankins, C., Samson, J., Bailey, A., Giaquinto, C., Ruga, E., De Rossi, A., Truscia, D., Grosch Worner, I., Schafer, A., Mok, J., Johnstone, F., Jiminez, J., de Alba, C., Garcia Rodriguez, M., Bates, I., de Jose, I., Hawkins, F., Zapico, R. M., Asensi Botet, F., Otero, M., Perez Tamarit, D., Moya, A., Galbis, M., Scherpbier, H., Boer, K., Bohlin, A., Lindgren, S., Ehrnst, A., Anzen, B., Belfrage, E., Levy, J., Alimenti, A., Barlow, P., Ferrazin, A., De Maria, A., Gotta, C., Maritati, V., Mur, A., Rovira, M., Paya, A., Coll, O., Fortuny, C., Boguna, J., Caro, M. C., Canet, Y., Ravizza, M., Castagna, C., Fiore, S., Guerra, B., Lanari, M., Bianchi, S., Bovicelli, L., Prati, E., Duse, M., Soresina, A., Scaravelli, G., De Santis, M., Muggiasca, M., Vigano, A., Marchisio, P., Iasci, A., Spinillo, A., Bucceri, A., Grossi, E., Rancilio, L., Della Torre, M., Dallacasa, P., Pachi, A., Principi, N., Zara, C., Vignali, M., Rossi, G., Selvaggi, L., Greco, P., Vimercati, A., Massi, G., Innocenti, T., Fiscella, A., Sansone, M., Benedetto, C., Tibaldi, C., Ziarati, N., Tadrist, B., Thevenicau, D., Gondry, J., Paulard, B., Alisy, C., Brault, D., Tordjeman, P., Mamou, J., Rozan, M., Colombani, D., Pincemaille, O., Salvetti, A., Chabanier, C., Hernandorena, X., Leroy, J., Schaal, J., Balde, P., Faucher, P., Lachassinne, E., Benoit, S., Douard, D., Hocke, C., Barjot, P., Brouard, J, Delattre, P, Stien, L, Audibert, F, Labrune, P, Vial, M, Mazy, F, Sitbon, D, Crenn Hebert, C, Floch Tudal, C, Akakpo, R, Daveau, C, Leblanc, A, Cesbron, P, Duval Arnould, H, Huraux Rendu, C, Lemerle, S, Touboul, C, Guerin, M, Maingueneau, C, Reynaud, I, Rousseau, T, Ercoil, V, Lanza, M, Denavit, M, Garnier, J, Lahsinat, K, Pia, R, Allouche, C, Nardou, M, Grall, F, May, A, Dallot, M, Lhuillier, P, Cecile, W, Mezin, R, Balde, P, Bech, A, Lobut, J, Algava, G, Dermesay, Ac, Busuttil, R, Jacquemot, M, Bader Meunier, B, Fridman, S, Codaccioni, X, Maxingue, F, Thomas, D, Alain, J, De Lumley, L, Tabaste, J, Salin, Pb, Seaume, H, Guichard, A, Kebaili, K, Roussouly, C, Botto, C, De Lanete, A, Wipff, P, Cravello, L, De Boisse, P, Leclaire, M, Michel, G, Crumiere, C, Lefevre, V, Le Lorier, B, Pauly, I, Robichez, B, Seguy, D, Dehlinger, M, Rideau, F, Talon, P, Benos, P, Huret, C, Nicolas, J, Heller Roussin, B, Saint Leger, S, Delaporte, M, Hubert, C, De Sarcus, B, Karoubi, P, Mechinaud, F, Bertcrottiere, D, Bongain, A, Monpoux, F, De Gennes, C, Devianne, F, Nisand, I, Rousset, M, Mouchnino, G, Muray, J, Munzer, M, Quereux, C, Brossard, V, Clavier, B, Allemon, M, Rotten, D, Stephan, J, Varlet, M, Guyot, B, Narey, P, Bardinet, F, De Caunes, F, Jeny, R, Robin, M, Bouley, Ar, Savey, L, Berrebi, A, Tricoire, J, Borderon, J, Fignon, A, Guillot, F, Maria, B, Broyard, A, Chitrit, Y, Firtion, G, Mandelbrot, L, Pillet, Ml, Parat, S, Boissinot, C, Garec, N, Levine, M, Ottenwalter, A, Schaller, F, Vilmer, B, Courpotin, C, Brunner, C, Ciraru Vigneron, N, Hatem Gantzer, G, Fritel, X, Wallet, A, Bouille, J, Milliez, J, Mrejen, Db, Dermer, E, Noseda, G, Bardou, D, Cressaty, J, Francoual, C, Moncomble, Cc, Cohen, H, Blanche, S, Bastion, H, Benifla, J, Benkhatar, F, Berkane, N, Herve, F, Ronzier, M, Mayaux, Mj, de Martino, M, Tovo, Pa, Galli, L, Gabiano, C, Ferraris, G, Rancillo, L, Bucceri, A, Tulisso, S, Scolfaro, C, Riva, C, Vierucci, A, de Luca, M, Farina, S, Fundaro, C, Genovese, O, Mercu, G, Forni, G, Stegagno, M, Falconieri, P, Zuccotti, G, Riva, E, Cellini, M, Baraldi, C, Consolini, R, Palla, G, Ruggeri, M, Pignata, Claudio, Guarino, Alfredo, Osimani, P, Metri, A, Antonellini, A, Benaglia, G, Romano, A, Dallacasa, P, De Mattia, D, Caselli, D, Boni, S, Dell'Erba, G, Bassanetti, F, Sticca, M, Timpano, C, Magnani, C, Salvatore, C, Gambaretto, G, Lipreri, R, Tornaghi, R, Pinzani, R, Cecchi, M, Bezzi, T, Battisti, L, Bresciani, E, Gattinara, G, Berrino, R, Pellegatta, A, Mazza, A, Baldi, F, Micheletti, E, Ruga, E, Altobelli, R, Deiana, M, Colnaghi, C, Tarallo, L, Tondo, U, Anastasio, E, Duse, M, Chiriaco, P, Contardi, I, Ruggeri, C, Ibba, P, Scott, G, Hutto, C, O'Sullivan, M, Malmsberry, A, Willoughby, A, Burns, D, Goedert, J, Landesman, S, Minkoff, H, Mendez, H, Holman, S, Rubinstein, A, Durako, S, Muenz, L, Goodwin, S, Nesheim, S, Lindsay, M, Clark, S, Lee, F, Nahmias, A, Sawyer, M, Vink, P, Farley, J, Alger, L, Abrams, E, Bamji, M, Lambert, G, Schoenbaum, E, Thea, D, Thomas, P, Weedon, J, Palumbo, P, Bardeguez, A, Denny, T, Oleske, J, Simonds, R, Orloff, S, Ethier Ives, J, Rogers, M, Schluchter, M, Kutner, M, Kaplan, S, Kattan, M, Lipshultz, S, Mellins, R, Shearer, W, Peavy, H, Sopko, G, Sloand, E, Wu, M, Kind, C, Nadal, D, Rudin, C, Siegrist, Ca, Wyler, Ca, Cheseaux, Jj, Aebi, C, Gnehm, H, Schubiger, G, Klingler, J, Hunziker, U, Kuchler, H, Gianinazzi, M, Buhlmann, U, Biedermann, K, Lauper, U, Irion, O, Brunelli, A, Spoletini, G, Schreyer, A, Hosli, I, Saurenmann, E, Drack, G, Isenschmid, M, Poorbeik, M, Schupbach, J, Perrin, L, Erb, P, Joller, H, Bryson, Y, Dillon, M, Nielsen, R, Boyer, P, Liao, D, Keller, M, Deveikis, A, Kovacs, A, Stek, A, Chan, L, Rother, C, Khoury, M, Diaz, C, Pacheco Acosta, E, Tuomala, R, Cooper, E, Mesthene, D, Pitt, J, Higgins, A, Moroso, G, Rich, K, Turpin, D, Cooper, N, Fowler, M, Nugent, R, Smeriglio, V, Mckinlay, S, Kalish, L, Ellis, S, and Andiman, W
- Abstract
To evaluate the relation between elective cesarean section and vertical transmission of human immunodeficiency virus type 1 (HIV-1), we performed a meta-analysis using data on individual patients from 15 prospective cohort studies. North American and European studies of at least 100 mother-child pairs were included in the meta-analysis. Uniform definitions of modes of delivery were used. Elective cesarean sections were defined as those performed before onset of labor and rupture of membranes. Multivariate logistic-regression analysis was used to adjust for other factors known to be associated with vertical transmission. The primary analysis included data on 8533 mother-child pairs. After adjustment for receipt of antiretroviral therapy, maternal stage of disease, and infant birth weight, the likelihood of vertical transmission of HIV-1 was decreased by approximately 50 percent with elective cesarean section, as compared with other modes of delivery (adjusted odds ratio, 0.43; 95 percent confidence interval, 0.33 to 0.56). The results were similar when the study population was limited to those with rupture of membranes shortly before delivery. The likelihood of transmission was reduced by approximately 87 percent with both elective cesarean section and receipt of antiretroviral therapy during the prenatal, intrapartum, and neonatal periods, as compared with other modes of delivery and the absence of therapy (adjusted odds ratio, 0.13; 95 percent confidence interval, 0.09 to 0.19). Among mother-child pairs receiving antiretroviral therapy during the prenatal, intrapartum, and neonatal periods, rates of vertical transmission were 2.0 percent among the 196 mothers who underwent elective cesarean section and 7.3 percent among the 1255 mothers with other modes of delivery. The results of this meta-analysis suggest that elective cesarean section reduces the risk of transmission of HIV-1 from mother to child independently of the effects of treatment with zidovudine.
- Published
- 1999
12. Rapid disease progression in HIV-1 perinatally infected children born to mothers receiving zidovudine monotherapy during pregnancy. The Italian register for HIV Infection in Children
- Author
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de Martino M, Galli L, Tovo P. A, Gabiano C, Osimani P, Zizzadoro P, de Mattia D, Ruggeri M, Lanari M, Dalla Vecchia S, Masi M, Miniaci A, Baldi F, Dell' Erba G, Battisti L, Duse M, Crispino P, Uberti E, Bresciani E, Chiriacò P. G, Pintor C, Dedoni M, Loriano D, Dessì C, Anastasio L, Sabatino G, Sticca M, Berrino R, Lodato A, Vierucci A, Farina S, de Luca M, de Maria A, Fioredda F, Boni S, Marazzi M. G, Pontali E, Forni GL, Gotta C, Tasso, L, Gambaretto G, Meo A, Plebani R, Pinzani R, Salvini F, Marchisio P, Massironi E, Tornaghi R, Zuccotti GV, Riva S, de Carlis S, Ferraris G, Bucceri A, Lipreri R, Cellini M, Tarallo L, Giaquinto C, Ruga E, Rampon O, Romano A, Benaglia G, Caselli D, Maccabruni A, Consolini R, Palla G, Antonellini A, Magnani C, Cecchi T, Castelli Gattinara G, Bernardi S, Cancrini C, Fundarò C, Genovese O, Rendeli C, Timpano C, Anzidei G, Catania S, Stegagno M, Mazza A, Salvatore C, Scolfaro C, Palomba E, Riva C, Pellegatta A., GUARINO, ALFREDO, PIGNATA, CLAUDIO, de Martino, M, Galli, L, Tovo, P. A., Gabiano, C, Osimani, P, Zizzadoro, P, de Mattia, D, Ruggeri, M, Lanari, M, Dalla Vecchia, S, Masi, M, Miniaci, A, Baldi, F, Dell' Erba, G, Battisti, L, Duse, M, Crispino, P, Uberti, E, Bresciani, E, Chiriacò, P. G., Pintor, C, Dedoni, M, Loriano, D, Dessì, C, Anastasio, L, Sabatino, G, Sticca, M, Berrino, R, Lodato, A, Vierucci, A, Farina, S, de Luca, M, de Maria, A, Fioredda, F, Boni, S, Marazzi, M. G., Pontali, E, Forni, Gl, Gotta, C, Tasso, L, Gambaretto, G, Meo, A, Plebani, R, Pinzani, R, Salvini, F, Marchisio, P, Massironi, E, Tornaghi, R, Zuccotti, Gv, Riva, S, de Carlis, S, Ferraris, G, Bucceri, A, Lipreri, R, Cellini, M, Guarino, Alfredo, Pignata, Claudio, Tarallo, L, Giaquinto, C, Ruga, E, Rampon, O, Romano, A, Benaglia, G, Caselli, D, Maccabruni, A, Consolini, R, Palla, G, Antonellini, A, Magnani, C, Cecchi, T, Castelli Gattinara, G, Bernardi, S, Cancrini, C, Fundarò, C, Genovese, O, Rendeli, C, Timpano, C, Anzidei, G, Catania, S, Stegagno, M, Mazza, A, Salvatore, C, Scolfaro, C, Palomba, E, Riva, C, and Pellegatta, A.
- Abstract
OBJECTIVE: To investigate the outcome in children perinatally infected with HIV-1 whose mothers received zidovudine (ZDV) monotherapy in pregnancy. DESIGN: Observational retrospective study of a prospectively recruited cohort. SETTING: Italian Register for HIV Infection in Children. PATIENTS: A group of 216 children perinatally infected with HIV-1, born in 1992-1997 and derived prospectively from birth: 38 children had mothers receiving ZDV monotherapy and for 178 children the mothers received no antiretroviral treatment during pregnancy. MAIN OUTCOME MEASURES: The estimated probability of developing severe disease or severe immune suppression, survival probability [95% confidence interval (CI)] within 3 years, and the hazard ratio (95% CI), adjusted for year of birth, maternal clinical condition at delivery, birthweight and treatments (Pneumocystis carinii pneumonia chemoprophylaxis and/or antiretroviral therapy before the onset of severe disease, severe immune suppression or death) were compared. RESULTS: Comparison of HIV-1-infected children whose mothers were treated with ZDV with children whose mothers were not treated showed that the former group had a higher probability of developing severe disease [57.3% (95% CI 40.9-74.3) versus 37.2% (95% CI 30.0-45.4); log-rank test 7.83, P = 0.005; adjusted hazard ratio 1.8 (95% CI 1.1-3.1)] or severe immune suppression [53.9% (95% CI 36.3-73.5) versus 37.5% (95% CI 30.0-46.2); log-rank test 5.58, P = 0.018; adjusted hazard ratio 2.4, (95% CI: 1.3-4.3)] and a lower survival [72.2% (95% CI 50.4-85.7) versus 81.0% (95% CI 73.7-86.5); log-rank test 4.23, P = 0.039; adjusted hazard ratio of death 1.9 (95% CI 1.1-3.6)]. CONCLUSIONS: This epidemiological observation could stimulate virologic studies to elucidate whether this rapid progression depends on in utero infection or transmission of resistant virus. Findings may suggest a need to hasten HIV-1 diagnosis in infants of ZDV-treated mothers and undertake an aggressive antiretroviral therapy in those found to be infected
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- 1999
13. Italian guidelines for antiretroviral therapy in children with human immunodeficiency virus-type 1 infection
- Author
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De Martino M., Tovo P., Giaquinto C., De Rossi A., Galli L., Gabiano C., Floridia M., Ferraris g., Ruga E., Castelli Gattinara G., Scolafaro C., Vierucci A., Viganò A., Zuccotti G. V., Fundarò C., Caselli D., De Maria A., Duse M., Plebani A., Timpano C., Lanari M., Stegagno M., Lipreri R., Gotta C., Ruggeri M., Loriao D., Osimani P., m. Cellini M., Forni G. L., Benaglia G., Antonellini A., De Mattia D., Pintor C., Mazza A., Romano A., Consolini R., Marazzi M. G., GUARINO, ALFREDO, Cecchi M. T., Zizzadoro P., Battisti L., Bezzi T., Salvatore C., Ciccia M., Bionda S., Metri A. M., Anastasio E., Magnani C., Sticca M., Tarallo L., Masi M., Gamberetto G., Catania S., Micheletti E., Pellegatta A., Dessi C., Baldi F., Ibba P., Bresciani E., Nasi C., Falconieri P., Tasso L., Altobelli R., Chiariacò P. G., Ruggeri C., Magni L. A., Tondo U., Contardi I., Tommasi D., Di Gregorio F., Meo A., Resta F., Molesini M., Sabatino G., Portelli V., PIGNATA, CLAUDIO, De Martino, M., Tovo, P., Giaquinto, C., De Rossi, A., Galli, L., Gabiano, C., Floridia, M., Ferraris, G., Ruga, E., Castelli Gattinara, G., Scolafaro, C., Vierucci, A., Viganò, A., Zuccotti, G. V., Fundarò, C., Caselli, D., De Maria, A., Duse, M., Plebani, A., Timpano, C., Lanari, M., Stegagno, M., Lipreri, R., Gotta, C., Ruggeri, M., Loriao, D., Osimani, P., m. Cellini, M., Forni, G. L., Benaglia, G., Antonellini, A., De Mattia, D., Pintor, C., Mazza, A., Romano, A., Consolini, R., Pignata, Claudio, Marazzi, M. G., Guarino, Alfredo, Cecchi, M. T., Zizzadoro, P., Battisti, L., Bezzi, T., Salvatore, C., Ciccia, M., Bionda, S., Metri, A. M., Anastasio, E., Magnani, C., Sticca, M., Tarallo, L., Masi, M., Gamberetto, G., Catania, S., Micheletti, E., Pellegatta, A., Dessi, C., Baldi, F., Ibba, P., Bresciani, E., Nasi, C., Falconieri, P., Tasso, L., Altobelli, R., Chiariacò, P. G., Ruggeri, C., Magni, L. A., Tondo, U., Contardi, I., Tommasi, D., Di Gregorio, F., Meo, A., Resta, F., Molesini, M., Sabatino, G., and Portelli, V.
- Abstract
Human immunodeficiency virus-type 1 (HIV-1) infection and its treatment are peculiar in children. Adherence and compliance must be carefully taken into account before initiating or changing therapy and in the choice of drugs. Even in the absence of paediatric-specific trial results and notwithstanding drug-labelling notations, all antiretroviral drugs should be used when indicated. A combined therapy is compulsory. Therapy is highly recommended in category C or category 3 and recommended in category B children. Indications in categories N1, N2, A1 or A2 are limited. A triple association is recommended in category C or category 3 children or in those with a high viral load, when compliance is guaranteed. A step-down strategy is not advisable. Infants' treatment should be inserted into controlled studies. Therapy should be changed when serious side effects or poor tolerance (choose drugs with a different toxicity and greater tolerance), poor compliance (individualize the motives) or treatment failure (evaluate progression and adherence) occurs.
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- 1999
14. La faringotonsillite e l'otite media acuta in età pediatrica. La guida rapida sviluppata dalla regione Emilia Romagna
- Author
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Gagliotti, S. Di Mario C., Asciano, M., Moro, M. L., Alboresi, S., Alonge, C., Amarri, S., Ballini, L., Benaglia, G., Bergamini, M., Bernardi, F., Bertolani, P., Borgna, Caterina, Boschi, G., Brunelli, A., Capra, L., Cicognani, A., Dallacasa, P., Dodi, I., Ferrari, A., Gregori, G., Lugli, N., Pocecco, M., Ponti, R., Reggiani, L., Tamburini, P., and Tomesani, A.
- Subjects
Sore throat ,Otitis ,Children ,Guideline ,Emilia-Romagna - Published
- 2008
15. Mother to child transmission of Hepatitis C Virus in a province of Northern Italy
- Author
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Licia VERONESI, Verrotti Di Pianella, C., Benassi, L., Benaglia, G., Affanni, P., and Tanzi, M. L.
- Subjects
virus diseases - Abstract
NTRODUCTION: Study reports of mother to child transmission of hepatitis C virus (HCV) have shown transmission rates ranging from 3 to 37%, according to maternal viremia and HIV-1 coinfection. The present study evaluated the prevalence of the HCV infection in the general population and the incidence of vertical transmission, from women who delivered in the Obstetric Clinic of the Hospital of Parma from January 1st 1996 to 31st 2001 December. METHODS: Mothers and children were tested for the presence of HCV-RNA within one week after delivery. Children were considered to be infected when they were found positive at least twice for viral RNA or antibodies were still detectable at the end of the follow-up period (18 months) in blood. RESULTS: Out of 13,025 women, 110 (0.8%) were found positive for anti-HCV antibodies; 72 of them (65.4%) were HCV-RNA positive. All 110 children were positive for anti-HCV antibodies in the first blood sample (time 0); 8 of them were HCV-RNA positive. Three children were still viremic at the end of the follow-up whereas 5 showed a clearance. No significant differences were found between viremic and nonviremic children with respect to gestational week, maternal alanine aminotransferase (ALT) levels and newborns weight at birth. CONCLUSION: This investigation shows that vertical transmission may occur in a general obstetric population despite a low prevalence of HCV-positive subjects., Journal of Preventive Medicine and Hygiene, Vol 48, No 2 (2007)
- Published
- 2007
- Full Text
- View/download PDF
16. Le infezioni da Streptococco beta emolitico di gruppo B: l’esperienza dell’Emilia-Romagna
- Author
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Berardi, A, Lugli, L, Benaglia, G., Cassani, C., Chiossi, C, Cipolloni, P, Falcioni, F, Gentili, A, Mantovani, A, Paltrinieri, G, Piccinini, L, Rossi, M. R., Rubbi, P, Simoni, A, and Somenzi, P.
- Published
- 2007
17. Onset of clinical signs in children with HIV-1 perinatal infection. Italian Register for HIV Infection in Children
- Author
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Galli L, de Martino M, Tovo PA, Gabiano C, Zappa M, Giaquinto C, Tulisso S, Vierucci A, Guerra M, Marchisio P, Plebani A, Zuccotti GV, Martino AM, Dallacasa P, Stegagno M, Belloni M, Castelli Gattinara G, Caselli D, Duse M, Corrias A, Consolini R, Cocchi P, Risso S, Ferraris G, Forni G. L, Mazzoni P. L, Portelli V, Benaglia G, Mazza A, Cellini M, Ruggeri M, De Mattia D, Falconieri P, Ciccimarra F, Bassanetti F, Anastasio E, Micheletti E, Masi M, Bresciani E, De Manzini A, Lusardi P, Baldi F, Eisenstecken M, Dell'Erba G, Sticca M, Berrino R., GUARINO, ALFREDO, Galli, L, de Martino, M, Tovo, Pa, Gabiano, C, Zappa, M, Giaquinto, C, Tulisso, S, Vierucci, A, Guerra, M, Marchisio, P, Plebani, A, Zuccotti, Gv, Martino, Am, Dallacasa, P, Stegagno, M, Belloni, M, Castelli Gattinara, G, Caselli, D, Duse, M, Corrias, A, Consolini, R, Cocchi, P, Risso, S, Ferraris, G, Forni, G. L., Mazzoni, P. L., Portelli, V, Benaglia, G, Mazza, A, Cellini, M, Guarino, Alfredo, Ruggeri, M, De Mattia, D, Falconieri, P, Ciccimarra, F, Bassanetti, F, Anastasio, E, Micheletti, E, Masi, M, Bresciani, E, De Manzini, A, Lusardi, P, Baldi, F, Eisenstecken, M, Dell'Erba, G, Sticca, M, and Berrino, R.
- Subjects
Male ,Acquired Immunodeficiency Syndrome ,Hepatitis, Viral, Human ,Infant, Newborn ,Infant ,CD4 Lymphocyte Count ,Cohort Studies ,Survival Rate ,AIDS-Related Complex ,Child, Preschool ,Diarrhea, Infantile ,Splenomegaly ,Disease Progression ,HIV-1 ,Birth Weight ,Humans ,Female ,Age of Onset ,Hepatomegaly ,Probability - Abstract
OBJECTIVE: To investigate the timing of onset of each clinical sign in infants and children with HIV-1 perinatal infection. DESIGN AND METHODS: A total of 200 HIV-1-infected children followed-up from birth were studied. Failure and conditional probabilities were estimated by the Kaplan-Meier product-limit method. Cox proportional hazard analysis was used to evaluate independently associated factors. Results of 934 seroreverters were used to calculate reference values of CD4+ cell counts and predictivity of early signs. RESULTS: Median age at the onset of any sign was 5.2 months (range, 0.03-56 months). The probability of remaining asymptomatic was 19% [95% confidence interval (CI), 14-25.1] at 12 months and 6.1% (95% CI, 2.6-11.7) at 5 years. Lymphadenopathy (69.5%), splenomegaly (62.4%) and hepatomegaly (58.4%) were the most common signs in the first year of life. Peculiar to the first year of life (compared with subsequent ages) was the onset of primary HIV-1 hepatitis and diarrhoea (rate ratios, 23.3 and 15.2, respectively). When CD4+ cell counts in the asymptomatic stage (age, 2 months; range, 0.03-5.9 months) were below rather than above the fifth percentile in seroreverters, onset of signs was earlier [3 range, 0.03-19) versus 5 (range, 0.03-56) months]. Children manifesting signs before the 5.2-month breakpoint had a lower survival rate [74% (range, 65.9-82%) at 12 months and 45% (range, 32.9-57%) at 5 years] than children manifesting signs later [98% (range, 92.2-100%) at 12 months and 74% (range, 60.3-87.7%) at 5 years]. Children whose birthweight was < or = 2400 g had an earlier onset (24 months; range, 1-57 months) of severe conditions than children with higher birthweight (71 months; range, 1-71 months). Development of lymphadenopathy or hepatosplenomegaly within 3 months of life were reliable indicators of infection. CONCLUSIONS: This study describes the sequence of onset of signs in perinatal HIV-1 infection. Infection is shown to progress faster than in adults and in a different manner. Low birthweight, early decreased CD4+ cell counts, and early onset of signs are predictive of rapid progression.
- Published
- 1995
18. Restoration of arylsulphatase A activity in murine metachromatic leukodistrophy oligodendrocytes by retroviral vector-mediated gene transfer
- Author
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Consiglio, A, Martino, Sabata, Dolcetta, D, Troiani, A, Severini, Gm, Benaglia, G, Wrabetz, L, Cusella, G, Orlacchio, Antonio, Orlacchio, A, Marchesini, S, Aebischer, P, and Bordignon, C.
- Published
- 2000
19. Human immunodeficiency virus-related cancer in children: Incidence and treatment outcome - Report of the Italian Register
- Author
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Author:, Caselli, Klersy, D, C, Martino, De, Gabiano, M, Galli, C, Tovo, L, Aricò, Pa, Group Author: Italian Register HIV Infect Children: Osimani, M., P, Bari, Di, Larovere, C, Ruggeri, D, Masi, M, Specchia, M, Battisti, F, Duse, L, Crispino, M, Carrara, P, Pintor, P, Dedoni, C, Dessì, M, Loriano, C, Anastasio, D, Bezzi, E, T, Luca, De, Farina, M, Vierucci, S, Bassetti, A, Pedemonte, D, Toscanini, P, Marazzi, F, Tasso, Mg, Plebani, L, Salvini, A, Pinzani, F, Bricalli, R, Viganò, D, Sala, A, Zuccotti, N, Riva, Gv, Giovannini, E, Liprieri, M, Conio, R, Ferraris, S, Cellini, G, Baraldi, C, Guarino, C, Bruzzese, A, Tarallo, E, Giaquinto, L, Giacomet, C, Rampon, V, O, Dalle, Nogare, Sanfilipp, Er, Romano, A, A, Benaglia, G, Dodi, I, Caselli, D, Maccabruni, A, Pacati, R, Consolini, Rita, and Legitimo, Annalisa
- Published
- 2000
20. Successful gene therapy approach for a murine model of MLD
- Author
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Consiglio, Antonella, Martino, S., Quattrini, A., Dolcetta, D., Bensadoun, J. C., Trojani, A., Benaglia, G., Marchesini, S., Cestari, V., and Naldini, L.
- Published
- 2000
21. LENTIVIRAL VECTOR FOR GENE THERAPY OF METACHROMATIC LEUCODYSTROPHY
- Author
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Consiglio, A., Martino, Sabata, Quattrini, A., Bensadoun, J. C., Dolcetta, D., Trojani, A., Cusella, G., Benaglia, G., Marchesini, S., Gieselmann, V., Naldini, L., and Bordignon, C.
- Published
- 2000
22. Mother to child transmission of Hepatitis C Virus in a province of Northern Italy
- Author
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Veronesi, Licia, Verrotti Di Pianella, C, Benassi, L, Benaglia, G, Affanni, Paola, Tanzi, Elisabetta, Veronesi, Licia, Verrotti Di Pianella, C, Benassi, L, Benaglia, G, Affanni, Paola, and Tanzi, Elisabetta
- Abstract
NTRODUCTION: Study reports of mother to child transmission of hepatitis C virus (HCV) have shown transmission rates ranging from 3 to 37%, according to maternal viremia and HIV-1 coinfection. The present study evaluated the prevalence of the HCV infection in the general population and the incidence of vertical transmission, from women who delivered in the Obstetric Clinic of the Hospital of Parma from January 1st 1996 to 31st 2001 December. METHODS: Mothers and children were tested for the presence of HCV-RNA within one week after delivery. Children were considered to be infected when they were found positive at least twice for viral RNA or antibodies were still detectable at the end of the follow-up period (18 months) in blood. RESULTS: Out of 13,025 women, 110 (0.8%) were found positive for anti-HCV antibodies; 72 of them (65.4%) were HCV-RNA positive. All 110 children were positive for anti-HCV antibodies in the first blood sample (time 0); 8 of them were HCV-RNA positive. Three children were still viremic at the end of the follow-up whereas 5 showed a clearance. No significant differences were found between viremic and nonviremic children with respect to gestational week, maternal alanine aminotransferase (ALT) levels and newborns weight at birth. CONCLUSION: This investigation shows that vertical transmission may occur in a general obstetric population despite a low prevalence of HCV-positive subjects.
- Published
- 2007
23. Fallout from Chernobyl. Not all health problems seen close to Chernobyl can be attributed to radiation
- Author
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Butturini, A., Izzi, G. C., Benaglia, G., Lloyd, D., Pass, B., and Gale, R. P.
- Subjects
Letter - Published
- 1994
24. EPIDEMIOLOGY OF HIV-INFECTION IN CHILDREN IN ITALY
- Author
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The Italian Register for HIV Infection in Children Coordinators: TOVO, Martino, De, Collaborators:, M, Gabiano, C, Galli, L, Caramia, G, Demattia, D, Desantis, U, Ruggeri, M, Zaniboni, Mg, Masi, M, Dellerba, G, Dallacasa, P, Baldi, F, Pescouderungg, L, Duse, M, Bresciani, E, Quarta, G, Dessi, C, Corrias, A, Chiappe, F, Ibba, P, Digregorio, F, Sciotto, A, Tarallo, L, Lauria, F, Sticca, M, Berrino, R, Bezzi, T, Mannelli, F, Cocchi, P, Bassetti, D, Boeri, E, Risso, S, Forni, Gl, Tondo, U, Micheletti, E, Gambaretto, G, Meo, A, Plebani, A, Magni, La, Marchisio, P, Zuccotti, Gv, Simoni, L, Stucchi, C, Ferraris, G, Altobelli, R, Mazzoni, Pl, Grandori, L, Ciccimarra, F, Esposito, L, Guarino, A, Bona, G, Giordano, S, Portelli, V, Giaquinto, C, Benaglia, G, Caselli, D, Bassanetti, F, and Consolini, Rita
- Published
- 1994
25. HIV-I INFECTION IN PERINATALLY EXPOSED SIBLINGS AND TWINS
- Author
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Martino, M., Tovo, P. -A, Galli, L., Caselli, D., Gabiano, C., Mazzoni, P. L., Giacomelli, A., Duse, M., Fundaro, C., Masi, M., Cocchi, P., Loy, A., Paola Giovanna Marchisio, Zuccotti, G. V., Conte, A., Casteili Gattinara, G., Bardare, M., Ferraris, G., Ciccimarra, F., Rizzi, M., Sciotto, A., Forni, L., Consolini, R., Benaglia, G., Bresciani, E., and Cutillo, S.
- Published
- 1991
26. Fallout from Chernobyl Thyroid cancer in children increased dramatically in Belarus
- Author
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Williams, E D, primary, Abelin, T, additional, Egger, M, additional, Ruchti, C, additional, Petridou, E, additional, Kampmann, B, additional, Sperling, K, additional, Pelz, J, additional, Wegner, R D, additional, Dorries, A, additional, Gruters, A, additional, Mikkelsen, M, additional, Butturini, A, additional, Izzi, G, additional, Benaglia, G, additional, Lloyd, D, additional, Pass, B, additional, Gale, R P, additional, Boice, J, additional, Linet, M, additional, Ambach, W, additional, Rehwald, W, additional, Auvinen, A, additional, Arvela, H, additional, Rahola, T, additional, Suomela, M, additional, Rytomaa, T, additional, Hakama, M, additional, Hakulinen, T, additional, and Soderman, B, additional
- Published
- 1994
- Full Text
- View/download PDF
27. Pulmonary syndrome and intravenous high-dose desferrioxamine
- Author
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Scanderbeg, A.Castriota, primary, Izzi, G.C., additional, Butturini, A., additional, and Benaglia, G., additional
- Published
- 1990
- Full Text
- View/download PDF
28. EPIDEMIOLOGY, CLINICAL FEATURES, AND PROGNOSTIC FACTORS OF PAEDIATRIC HIV INFECTION
- Author
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Tovo, P. A., de Martino, M., Caramia), G., Armenio, L., Schettini, F., De Mattia, D., Chiodo, F., Masi, M., Trombacco, M. G., Zaniboni, M. G., Duse, Marzia, Vertua, G., Quarta, G., Cao, A., Dessi', C., Di Gregorio, F., Bezzi, T., Cocchi, P., Calabri, G., Vierucci, A., Galli, L., Boeri, E., Jannuzzi, C., Terragna, A., De Maria, A., Sanpietro, F., Barbanera, M., Bardare, M., Plebani, A., Giovannini, M., Magni, L. A., Marchisio, P., Tornaghi, R., Rossi, A., Esposito, L., Guarino, A., Romano, G., Viggiano, D., Zacchello, F., Giaquinto, C., Chieco Bianchi, L., Benaglia, G., Bertolini, P., Arico', M., Caselli, D., Bassanetti, F., Consolini, R., Antonellini, A., Magnani, C., Calvani, M., Falconieri, P., Segni, G., Fundaro', C., Gabiano, C., Palomba, E., Perugini, L., and Negro, F.
- Subjects
Hepatitis ,Pediatrics ,medicine.medical_specialty ,Pregnancy ,business.industry ,Vaginal delivery ,Mortality rate ,Secondary infection ,General Medicine ,medicine.disease ,Substance abuse ,Epidemiology ,medicine ,Lymphoid interstitial pneumonia ,business - Abstract
486 children born to HIV-positive mothers, 57 children infected by blood products, and 1 child for whom the personal history was not available were studied. Perinatal infection had a more varied clinical picture and a worse outcome compared with infection acquired later in childhood. Severe secondary infections, neurological disorders, and hepatitis (but not lymphoid interstitial pneumonia) were linked to a high mortality rate in perinatally infected children, in whom an early onset of symptoms was also a bad prognostic factor. Perinatal HIV infection occurred in 32·6% of children born to seropositive mothers, with a higher transmission rate in children born by vaginal delivery and then breast-fed. Preterm delivery and low birthweight seemed to be related to drug abuse during pregnancy, not to intrauterine HIV infection. Girls had a higher rate of perinatal infection and, of those infected, had an increased mortality.
- Published
- 1988
- Full Text
- View/download PDF
29. Nerve growth factor induces sphingomyelin accumulation in pheochromocytoma cells
- Author
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Piccinotti, A., Benaglia, G., Bresciani, R., Zizioli, D., Presta, M., Preti, A., and Marchesini, S.
- Published
- 2000
- Full Text
- View/download PDF
30. EPIDEMIOLOGY, CLINICAL-FEATURES, AND PROGNOSTIC FACTORS OF PEDIATRIC HIV INFECTION
- Author
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Italian Multicentre Study Coordinators: Tovo, Martino, De, M, Partecipants:, Caramia, Armenio, G, Schettini, L, F, Mattia, De, Chiodo, D, Masi, F, Trombacco, M, Zamboni, Mg, Duse, M, Vertua, M, Qyarta, G, Cao, G, Dessì, A, Gregorio, Di, Bezzi, F, Cocchi, T, Calabri, P, Vierucci, G, Galli, A, Boeri, L, Jannuzzi, E, Terragna, C, A, Maria, De, Sampietro, Ma, Barbanera,, Bardare, M, Plebani, M, Giovannini, A, Magni, M, Marchisio, La, Tornaghi, R, Rossi, R, Esposito, A, Guarino, L, Romano, A, Viggiano, G, Zacchello, D, Giaquinto, C, C, Chieco Bianchi, L, Benaglia, G, Bertyolini, G, Aricò, M, Caselli, D, Bassanetti, F, and Consolini, Rita
- Published
- 1988
31. Predictive value of the HIV paediatric classification system for the long-term course of perinatally infected children
- Author
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Galli, L., Martino, M., Tovo, P. -A, Gabiano, C., Zappa, M., Osimani, P., Mattia, D., Zizzadoro, P., Ruggeri, M., Baldi, F., Ciccia, M., Dallacasa, P., Masi, M., Battisti, L., Bresciani, E., Duse, M., Timpano, S., Chiriacò, P. G., Belloni, M., Corrias, A., Ibba, P., Rossi, G., Anastasio, E., Sabatino, G., Sticca, M., Nasi, C., Bezzi, T., Vierucci, A., Farina, S., Ballotti, S., Bassetti, D., Maria, A., Forni, G. L., Gotta, C., Marazzi, M. G., Mecca, D., Tasso, L., Tondo, U., Micheletti, E., Gambaretto, G., Cellini, M., Altobelli, R., Bucceri, A., Conio, S., Ferraris, G., Giovannini, M., Lipreri, R., Paola Giovanna Marchisio, Massironi, E., Pinzani, R., Plebani, A., Rancilio, L., Riva, E., Salvini, F., Tornaghi, R., Zuccotti, G. V., Guarino, A., Pignata, C., Giaquinto, C., Rampon, O., Ruga, E. M., Romano, A., Benaglia, G., Caselli, D., Maccabruni, A., Bassanetti, F., Consolini, R., Palla, G., Antonellini, A., Metri, A. M., Magnani, C., Cecchi, M. T., Castelli Gattinara, G., Catania, S., Falconieri, P., Fundarò, C., Genovese, O., Krzisztofiak, A., Livadiotti, S., Rendeli, C., Stegagno, M., Timpano, C., Mazza, A., Salvatore, C. M., Palomba, E., Riva, C., Tulisso, S., and Pellegatta, A.
32. Determinants of mother-to-infant human immunodeficiency virus 1 transmission before and after the introduction of zidovudine prophylaxis
- Author
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Martino, M., Galli, L., Tovo, P. -A, Gabiano, C., Pezzotti, P., Wagner, T. M., Rezza, G., Osimani, P., Mattia, D., Di Bari, C., Ruggeri, M., Baldi, F., Ciccia, M., Lanari, M., Masi, M., Venturi, V., Battisti, L., Duse, M., Chiriacò, P. G., Cavallini, R., Dessí, C., Pintor, C., Anastasio, E., Sabatino, G., Sticca, M., Pomero, G., Bezzi, T., Chiappini, E., Luca, M., Gervaso, P., Cecchi, M. T., Bassetti, D., Gotta, C., Rosso, R., Timitilli, A., Tondo, U., Mussini, P., Bricalli, D., Bucceri, A., Ferraris, G., Giovannini, M., Mosca, F., Lipreri, R., Plebani, A., Riva, E., Riva, S., Viganò, A., Gianvincenzo Zuccotti, Cellini, M., Buffolano, W., Guarino, A., Tarallo, L., D Elia, R., Giaquinto, C., Rampon, O., Dalle Nogare, E. R., Romano, A., Caselli, D., Maccabruni, A., Consolini, R., Benaglia, G., Magnani, C., Anzidei, G., Casadei Pistilli, A. M., Castelli Gattinara, G., Catania, S., Facente, C., Falconieri, P., Fundarò, C., Genovese, O., Rendeli, C., Bionda, S., Cristiano, L., Garetto, S., Riva, C., Palomba, E., Portelli, V., Mazza, A., Salvatore, C., Pellegatta, A., and Molesini, M.
33. Mode of delivery and gestational age influence perinatal HIV-1 transmission
- Author
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Pier Angelo Tovo, Martino, M., Gabiano, C., Galli, L., Cappello, N., Ruga, E., Tulisso, S., Vierucci, A., Loy, A., Zuccotti, G. V., Bucceri, A. M., Plebani, A., Marchisio, P., Caselli, D., Liviadotti, S., Dallacasa, P., Fundaró, C., Stegagno, M., Timpano, C., Ruggeri, M., Duse, M., Belloni, M., Cocchi, P., Risso, S., Forni, G. L., Lipreri, R., Ciccimarra, F., Consolini, R., Benaglia, G., Caramia, G., Santis, U., Chiriacó, R. G., Dessí, C., Ibba, P., Zannino, L., Gregorio, F. D., Sciotto, A., Cecchi, M. T., Boeri, E., Meo, A., Magni, L. A., Altobelli, R., Contardi, I., Gambaretto, G., Esposito, L., Bona, G., Giordano, S., Ragazzini, I., Magnani, C., Bionda, S., and Pellegatta, A.
34. Reduction in mortality with availability of antiretroviral therapy for children with perinatal HIV-1 infection
- Author
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Martino, M., Tovo, P. -A, Balducci, M., Galli, L., Gabiano, C., Rezza, G., Pezzotti, P., Osimani, P., Di Bari, C., Larovere, D., Ruggeri, M., Masi, M., Specchia, F., Battisti, L., Duse, M., Crispino, P., Carrara, P., Pintor, C., Dedoni, M., Dessì, C., Loriano, D., Anastasio, E., Bezzi, T., Luca, M., Farina, S., Vierucci, A., Bassetti, D., Pontali, E., Boni, S., Marazzi, M. G., Tasso, L., Giovanettoni, C., Salvini, F., Pinzani, R., Marchisio, P., Viganò, A., Tornaghi, R., Gianvincenzo Zuccotti, Riva, E., Giovannini, M., Lipreri, R., Conio, S., Ferraris, G., Cellini, M., Baraldi, C., Guarino, A., Canani, R. B., Tarallo, L., Giaquinto, C., Ruga, E., Rampon, O., Nogare, E. R. D., Sanfilippo, A., Romano, A., Benaglia, G., Dodi, I., Caselli, D., Maccabruni, A., Pacati, I., Consolini, R., Palla, G., Cecchi, M. T., Vecchi, V., Anzidei, G., Cerilli, S., Chiodi, R., Gattinara, G. C., Krzysztofiak, A., Bernardi, S., Fundarò, C., Genovese, O., Colafati, G. S., Catania, S., Ajassa, C., Mazza, A., Garetto, S., Riva, C., and Scolfaro, C.
35. Antiretroviral treatment in children with HIV-1 infection: Consensus of the Italian register for HIV infection in children | Trattamento antiretrovirale in bambini con infezione da HIV-1: Consensus del registro Italiano per l'infezione da HIV in pediatria
- Author
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Martino, M., Pier Angelo Tovo, Giaquinto, C., Rossi, A., Galli, L., Gabiano, C., Floridia, M., Ferraris, G., Ruga, E., Castelli Gattinara, G., Scolfaro, C., Vierucci, A., Viganò, A., Zuccotti, G. V., Fundarò, C., Caselli, D., Maria, A., Duse, M., Plebani, A., Timpano, C., Lanari, M., Stegagno, M., Lipreri, R., Gotta, C., Ruggeri, M., Loriano, D., Osimani, P., Cellini, M., Forni, G. L., Benaglia, G., Antonellini, A., Mattia, D., Pintor, C., Mazza, A., Romano, A., Consolini, R., Pignata, C., Marazzi, M. G., Guarino, A., Cecchi, M. T., Zizzadoro, P., Battisti, L., Bezzi, T., Salvatore, C., Ciccia, M., Bionda, S., Metri, A. M., Anastasio, E., Magnani, C., Sticca, M., Tarallo, L., Masi, M., Gambaretto, G., Catania, S., Micheletti, E., Pellegatta, A., Dessì, C., Baldi, F., Ibba, P., Bresciani, E., Berrino, R., Falconieri, P., Tasso, L., Altobelli, R., Chiriacò, P. G., Ruggeri, C., Magni, L. A., Tondo, U., Contardi, I., Tommasi, D., Di Gregorio, F., Meo, A., Resta, F., Molesini, M., Sabatino, G., and Portelli, V.
36. One year experience at the emergency unit of the Children's Hospital of Parma
- Author
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Giacalone, T., Vanelli, M., Zinelli, C., Ndongko, A., Ndaka, J., Casadei, A., Nicoli, D., Agnetti, A., Ammenti, A., Benaglia, G., Bertolini, P., Caffarelli, C., Capone, C., Carano, N., Chiari, G., Angelis, G. L., Fanti, A., ICILIO DODI, Ghizzoni, L., Grzincich, G. L., Pisi, G., Sani, E., Terzi, C., Tondelli, T., Virdis, R., and Zavota, L.
- Subjects
Male ,Adolescent ,Urban Population ,Infant ,Workload ,Hospitals, Pediatric ,Gastroenteritis ,Catchment Area, Health ,Italy ,Child, Preschool ,Humans ,Wounds and Injuries ,Female ,Seasons ,Emergencies ,Child ,Emergency Service, Hospital ,Respiratory Tract Infections ,Diagnosis-Related Groups ,Retrospective Studies - Abstract
The objective of the study was to analyse the activity of the Paediatric Emergency Unit (PEU) of the Children's Hospital in Parma, Italy, in the first year of its functioning. To this aim, the child's chronological age, place of origin (town or province), ethnic group, cause of consultation, time and date of admission, diagnosis and final destination were retrospectively collected from the clinical notes of all children who attended PEU from 1st. 10.1998 to 30th. 09.1999. During the period of this study 8,564 medical consultations (57% of users were male) were carried out by the Paediatricians on duty in the EU of The Children's Hospital. The average age of the patients was 3.9 +/- 3.5 years. Only 7% of patients passed through the General Emergency Department of the same Hospital. The peak period of consultations was found to be in February. The number of daily attendances progressively increased from Monday to Sunday according to a r of 0.59 (p0.02) with a peak during the weekend. The most frequent causes for attendance concerned infections in the upper respiratory tract (36%), gastroenteritis (22%) and injuries (12%). Attendance, consultation and discharge procedures were covered at an average interval of 36.1 +/- 15.6 minutes (median 30 min.). Seventy per cent of the patients were discharged, 56.7% were males. Fourteen per cent of the rest were admitted for a short period of observation in the beds of the PEU and 16% in beds of specialised wards in the PD. Eighty per cent of admissions at the PEU lasted less than 48 hours. The analysis of the data collected at the PEU of our PD during the first year of its activity highlights the huge amount of work carried out by the Paediatricians on duty. To solve the abnormal admittance to a PEU, a complete reorganization of the Family Paediatricians network has to be hoped for. Special attention must also be addressed to the users of a PEU in order to reduce their attendance. To reach this target a continuous health education and information program for the general population and first-time parents has to be planned. Beyond these considerations, there is not doubt that a PEU requires a specific medical and nursing staff in order to prevent the service becoming ineffective.
37. Long-term follow-up of HIV-seropositive children
- Author
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Pier Angelo Tovo, Martino, M., Gabiano, C., Galli, L., Ferraris, G., Giaquinto, C., Castelli Gattinara, G., Garetto, S., Vierucci, A., Marchisio, P., Zuccotti, G. V., Fundarò, C., Duse, M., Caselli, D., Maria, A., Plebani, A., Timpano, C., Lanari, M., Stegagno, M., Ruggeri, M., Gotta, G., Lipreri, L., Cellini, M., Osimani, P., Loriano, D., Benaglia, G., Mattia, D., Forni, G. L., Romano, A., Antonellini, A., Pintor, C., Consolini, R., Mazza, A., Micco, A., Guarino, A., Marazzi, M. G., Cecchi, M. T., Di Bari, C., Dessì, C., Bezzi, T., Bionda, S., Erba, G., Battisti, L., Salvatore, C., Sticca, M., Anastasio, E., Masi, M., Magnani, C., Tarallo, L., Gambaretto, G., Catania, S., Pellegatta, A., Todeschini, A., Baldi, F., Nasi, C., Bresciani, E., Tasso, L., Molesini, M., Chiriacò, P. G., Ruggeri, C., Stucchi, C., Resta, F., Sabatino, G., Delle Nogare, E., Tondo, U., Tommasi, D., Meo, A., Contardi, I., and Portelli, V.
38. V/Q IMBALANCE AND RESPONSE TO OXYGEN ADMINISTRATION IN CYSTIC FIBROSIS, ASTHMA AND PNEUMONIA
- Author
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Battistini, A, primary, Grzincich, Gl, additional, and Benaglia, G, additional
- Published
- 1975
- Full Text
- View/download PDF
39. Ganglioside GM2 is substrate for a sialidase in MDCK cells
- Author
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Marchesini, S., Benaglia, G., Piccinotti, A., Bresciani, R., and Preti, A.
- Published
- 1998
- Full Text
- View/download PDF
40. Role of emotional support in kwashiorkor.
- Author
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Virdis, R, Yussuf, M A, and Benaglia, G
- Subjects
- *
KWASHIORKOR , *CHILD care , *SOCIAL context , *PSYCHOLOGY - Published
- 1994
- Full Text
- View/download PDF
41. Characterization of three sialidases from Danio rerio
- Author
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Matilde Forcella, Eugenio Monti, Roberto Bresciani, Giuseppe Borsani, Nadia Papini, Paola Fusi, Giuliana Benaglia, Edoardo Giacopuzzi, Marta Manzoni, Marcella Bonanomi, Forcella, M, Manzoni, M, Benaglia, G, Bonanomi, M, Giacopuzzi, E, Papini, N, Bresciani, R, Fusi, P, Borsani, G, and Monti, E
- Subjects
0301 basic medicine ,Aggregation in solution ,In silico gene expression ,Molecular modelling ,Recombinant sialidases ,Substrate specificities ,Animals ,Humans ,Neuraminidase ,Protein Domains ,Recombinant Proteins ,Zebrafish Proteins ,Protein Multimerization ,Zebrafish ,Pentamer ,In silico ,Beta sheet ,Sialidase ,Antiparallel (biochemistry) ,Biochemistry ,NEU2 ,03 medical and health sciences ,030102 biochemistry & molecular biology ,biology ,Chemistry ,Recombinant sialidase ,Active site ,General Medicine ,biology.organism_classification ,030104 developmental biology ,biology.protein ,Substrate specificitie - Abstract
Zebrafish encodes several sialidases belonging to the NEU3 group, the plasma membrane-associated member of the family with high specificity toward ganglioside substrates. Neu3.1, Neu3.2 and Neu 3.3 have been expressed in E. coli and purified using the pGEX-2T expression system. Although all the enzymes are expressed by bacterial cells, Neu3.1 formed insoluble aggregates that hampered its purification. Neu3.2 and Neu3.3 formed oligomers as demonstrated by gel filtration chromatography experiments. Actually, the first formed a trimer whereas the second a pentamer. Intriguingly, despite relevant degree of sequence identity and similarity, the two enzymes showed peculiar substrate specificities toward gangliosides other than GM3, two glycoproteins and two forms of sialyllactose. Using molecular modelling and the crystal structure of the human cytosolic sialidase NEU2 as a template, the 3D models of the sialidases from zebrafish have been generated. As expected, the 3D models showed the typical six blade beta-propeller typical of sialidases, with an overall highly conserved active site architecture. The differences among the three zebrafish enzymes and human NEU2 are mainly located in the loops connecting the antiparallel beta strands of the propeller core. These portions of the proteins are probably responsible for the differences observed in substrate specificities, as well as in the different subcellular localization and aggregation features observed in solution. Finally, the in silico analysis of RNA-Seq data evidenced a peculiar expression profile of the three genes during embryogenesis, suggesting different roles of these sialidases during development.
- Published
- 2021
42. Metabolic correction in oligodendrocytes derived from metachromatic leukodystrophy mouse model by using encapsulated recombinant myoblasts
- Author
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Sergio Marchesini, Massimo Conese, Nicole Déglon, Antonella Consiglio, Claudio Bordignon, Sabata Martino, Patrick Aebischer, Lawrence Wrabetz, Gabriella Cusella, Giuliana Benaglia, Giovanni Maria Severini, D. Dolcetta, Aldo Orlacchio, Consiglio, A., Martino, S., Dolcetta, D., Cusella, G., Conese, M., Marchesini, S., Benaglia, G., Wrabetz, L., Orlacchio, A., Déglon, N., Aebischer, P., Severini, G. M., and Bordignon, Claudio
- Subjects
Capsules/therapeutic use ,Arylsulfatase A ,Polymers ,Myoblasts ,Genetic Vectors/*genetics ,Mice ,Transduction, Genetic ,Myoblasts/enzymology/*transplantation ,Transgenes ,Arylsulfatases ,Mice, Knockout ,Cell Survival/physiology ,Oligodendrocytes ,Genetic/*methods ,Graft Survival ,Metachromatic/enzymology/genetics/*therapy ,Cell encapsulation ,Graft Survival/physiology ,Up-Regulation ,Cell biology ,Oligodendroglia ,Treatment Outcome ,medicine.anatomical_structure ,Neurology ,Biochemistry ,Neuroglia ,MLD mouse model ,C2C12 ,Nerve Regeneration/genetics ,Gene therapy ,Cell Survival ,Transgenes/genetics ,Knockout ,Genetic Vectors ,Transplantation, Heterologous ,Capsules ,Biology ,Cell Line ,Viral vector ,Up-Regulation/genetics ,Transduction ,medicine ,Animals ,Humans ,Viability assay ,Polymers/therapeutic use ,Transplantation ,Sulfoglycosphingolipids ,Animal ,Leukodystrophy ,Leukodystrophy, Metachromatic ,medicine.disease ,Heterologous/methods ,Nerve Regeneration ,Metachromatic leukodystrophy ,Disease Models, Animal ,Oligodendroglia/*enzymology ,Sulfoglycosphingolipids/metabolism ,Cell culture ,Disease Models ,Arylsulfatases/genetics/metabolism/secretion ,Neurology (clinical) - Abstract
In an effort to develop an encapsulated cell-based system to deliver arylsulfatase A (ARSA) to the central nervous system of metachromatic leukodystrophy (MLD) patients, we engineered C2C12 mouse myoblasts with a retroviral vector containing a full-length human ARSA cDNA and evaluated the efficacy of the recombinant secreted enzyme to revert the MLD phenotype in oligodendrocytes (OL) of the As2-/- mouse model. After transduction, C2C12 cells showed a fifteen-fold increase in intracellular ARSA activity and five-fold increase in ARSA secretion. The secreted hARSA collected from transduced cells encapsulated in polyether-sulfone polymer, was taken up by enzyme-deficient OL derived from MLD mice and normally sorted to the lysosomal compartment, where transferred enzyme reached 80% of physiological levels, restoring the metabolism of sulfatide. To evaluate whether secreted enzyme could restore metabolic function in the brain, encapsulated cells and secreted ARSA were shown to be stable in CSF in vitro. Further, to test cell viability and enzyme release in vivo, encapsulated cells were implanted subcutaneously on the dorsal flank of DBA/2J mice. One month later, all retrieved implants released hARSA at rates similar to unencapsulated cells and contained well preserved myoblasts, demonstrating that encapsulation maintains differentiation of C2C12 cells, stable transgene expression and long-term cell viability in vivo. Thus, these results show the promising potential of developing an ARSA delivery system to the CNS based on the use of a polymer-encapsulated transduced xenogenic cell line for gene therapy of MLD.
- Published
- 2007
- Full Text
- View/download PDF
43. In vivo gene therapy of metachromatic leukodystrophy by lentiviral vectors: correction of neuropathology and protection against learning impairments in affected mice
- Author
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Antonella Consiglio, D. Dolcetta, Jean Charles Bensadoun, Claudio Bordignon, Sabata Martino, Luigi Naldini, Alberto Oliverio, Angelo Quattrini, Giuliana Benaglia, Alessandra Trojani, Sergio Marchesini, Vincenzo Cestari, Consiglio, A., Quattrini, A, Martino, S., Bensadoun, J. C., Dolcetta, D., Trojani, A., Benaglia, G., Marchesini, S., Cestari, V., Oliviero, A, Bordignon, Claudio, and Naldini, Luigi
- Subjects
Arylsulfatase A ,Genetic enhancement ,Central nervous system ,Genetic Vectors ,lysosomal enzymes ,Neuropathology ,Biology ,Cerebroside-Sulfatase/genetics/metabolism ,arylsulfatase A activity ,General Biochemistry, Genetics and Molecular Biology ,Brain/enzymology/metabolism/pathology ,Mice ,In vivo ,medicine ,Animals ,Humans ,Metachromatic/complications/pathology/*therapy ,Gene ,Cerebroside-Sulfatase ,lipid metabolic restoration ,Learning Disabilities ,Leukodystrophy ,Lentivirus ,Brain ,General Medicine ,Lentivirus/*genetics ,Genetic Therapy ,Leukodystrophy, Metachromatic ,medicine.disease ,Lipid Metabolism ,gene therapy ,Metachromatic leukodystrophy ,medicine.anatomical_structure ,Immunology ,Cancer research ,Learning Disorders/etiology/*prevention & control - Abstract
Metachromatic leukodystrophy (MLD) is a lipidosis caused by deficiency of arylsulfatase A (ARSA). Although the genetics of MLD are known, its pathophysiology is not understood. The disease leads to progressive demyelination and early death and no effective treatment is available. We used lentiviral vectors to deliver a functional ARSA gene (human ARSA) into the brain of adult mice with germ-line inactivation of the mouse gene encoding ARSA, As2. We report sustained expression of active enzyme throughout a large portion of the brain, with long-term protection from development of neuropathology and hippocampal-related learning impairments. We show that selective degeneration of hippocampal neurons is a central step in disease pathogenesis, and provide evidence that in vivo transfer of ARSA by lentiviral vectors reverts the disease phenotype in all investigated areas. Therefore, in vivo gene therapy offers a unique option for MLD and other storage diseases affecting the central nervous system.
- Published
- 2001
44. Long-term nonprogressors among children with perinatal HIV-1 infection
- Author
-
P. A. Tovo, M. de Martino, C. Gabiano, L. Galli, G. Ferraris, P. Marchisio, C. Giaquinto, S. Tulisso, G. V. Zuccotti, A. Loy, A. Vierucci, G. Castelli Gattinara, D. Caselli, A. Plebani, C. fundarò, P. Dallacasa, M. Belloni, P. L. Mazzoni, C. Timpano, M. Ruggeri, R. Consolini, G. L. Forni, G. Benaglia, M. G. Marazzi, M. Cellini, A. Mazza, C. Pignata, V. Portelli, D. De Mattia, A. Corrias, C. Gotta, T. Bezzi, G. Caramia, A. Antonellini, E. Anastasio, G. gambaretto, C. Salvatore, L. Battisti, M. Sticca, M. Masi, P. Ibba, S. Bionda, U. De Sarntis, F. Baldi, C. Magnani, A. Pellegatta, R. Berrino, C. Dessì, L. Tasso, L. A. Magni, L. Tarallo, C. Ajassa, U. Tondo, P. G. Chiriacò, I. Contardi, A. Meo, F. Di Gregorio, P. Paolucci, L. Esposito, E. Boeri, D. Tommasi, GUARINO, ALFREDO, Tovo, P. A., de Martino, M., Gabiano, C., Galli, L., Ferraris, G., Marchisio, P., Giaquinto, C., Tulisso, S., Zuccotti, G. V., Loy, A., Vierucci, A., Castelli Gattinara, G., Caselli, D., Plebani, A., Fundarò, C., Dallacasa, P., Belloni, M., Mazzoni, P. L., Timpano, C., Ruggeri, M., Consolini, R., Forni, G. L., Benaglia, G., Marazzi, M. G., Cellini, M., Mazza, A., Pignata, C., Portelli, V., De Mattia, D., Corrias, A., Guarino, Alfredo, Gotta, C., Bezzi, T., Caramia, G., Antonellini, A., Anastasio, E., Gambaretto, G., Salvatore, C., Battisti, L., Sticca, M., Masi, M., Ibba, P., Bionda, S., De Sarntis, U., Baldi, F., Magnani, C., Pellegatta, A., Berrino, R., Dessì, C., Tasso, L., Magni, L. A., Tarallo, L., Ajassa, C., Tondo, U., Chiriacò, P. G., Contardi, I., Meo, A., Di Gregorio, F., Paolucci, P., Esposito, L., Boeri, E., and Tommasi, D.
- Published
- 1997
45. Downregulation of Zebrafish Cytosolic Sialidase Neu3.2 Affects Skeletal Muscle Development.
- Author
-
Zizioli D, Codenotti S, Benaglia G, Manzoni M, Massardi E, Fanzani A, Borsani G, and Monti E
- Subjects
- Animals, Down-Regulation, Muscle, Skeletal, Muscle Development genetics, Neuraminidase genetics, Zebrafish, Zebrafish Proteins genetics
- Abstract
Sialidases remove terminal sialic acids residues from the non-reducing ends of glycoconjugates. They have been recognized as catabolic enzymes that work within different subcellular compartments and can ensure the proper turn-over of glycoconjugates. Four mammalian sialidases (NEU1-4) exist, with different subcellular localization, pH optimum and substrate specificity. In zebrafish, seven different sialidases, with high homology to mammalian counterparts, have been identified. Zebrafish Neu3.2 is similar to the human cytosolic sialidase NEU2, which is involved in skeletal muscle differentiation and exhibits a broad substrate specificity toward gangliosides and glycoproteins. In zebrafish neu3.2 , mRNA is expressed during somite development, and its enzymatic activity has been detected in the skeletal muscle and heart of adult animals. In this paper, 1-4-cell-stage embryos injected with neu3.2 splice-blocking morpholino showed severe embryonic defects, mainly in somites, heart and anterior-posterior axis formation. Myog and myod1 expressions were altered in morphants, and impaired musculature formation was associated with a defective locomotor behavior. Finally, the co-injection of Neu2 mouse mRNA in morphants rescued the phenotype. These data are consistent with the involvement of cytosolic sialidase in pathologies related to muscle formation and support the validity of the model to investigate the pathogenesis of the diseases.
- Published
- 2023
- Full Text
- View/download PDF
46. Characterization of three sialidases from Danio rerio.
- Author
-
Forcella M, Manzoni M, Benaglia G, Bonanomi M, Giacopuzzi E, Papini N, Bresciani R, Fusi P, Borsani G, and Monti E
- Subjects
- Animals, Humans, Neuraminidase genetics, Protein Domains, Recombinant Proteins chemistry, Recombinant Proteins genetics, Zebrafish Proteins genetics, Neuraminidase chemistry, Protein Multimerization, Zebrafish, Zebrafish Proteins chemistry
- Abstract
Zebrafish encodes several sialidases belonging to the NEU3 group, the plasma membrane-associated member of the family with high specificity toward ganglioside substrates. Neu3.1, Neu3.2 and Neu 3.3 have been expressed in E. coli and purified using the pGEX-2T expression system. Although all the enzymes are expressed by bacterial cells, Neu3.1 formed insoluble aggregates that hampered its purification. Neu3.2 and Neu3.3 formed oligomers as demonstrated by gel filtration chromatography experiments. Actually, the first formed a trimer whereas the second a pentamer. Intriguingly, despite relevant degree of sequence identity and similarity, the two enzymes showed peculiar substrate specificities toward gangliosides other than GM3, two glycoproteins and two forms of sialyllactose. Using molecular modelling and the crystal structure of the human cytosolic sialidase NEU2 as a template, the 3D models of the sialidases from zebrafish have been generated. As expected, the 3D models showed the typical six blade beta-propeller typical of sialidases, with an overall highly conserved active site architecture. The differences among the three zebrafish enzymes and human NEU2 are mainly located in the loops connecting the antiparallel beta strands of the propeller core. These portions of the proteins are probably responsible for the differences observed in substrate specificities, as well as in the different subcellular localization and aggregation features observed in solution. Finally, the in silico analysis of RNA-Seq data evidenced a peculiar expression profile of the three genes during embryogenesis, suggesting different roles of these sialidases during development., (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
47. Human sialic acid acetyl esterase: Towards a better understanding of a puzzling enzyme.
- Author
-
Orizio F, Damiati E, Giacopuzzi E, Benaglia G, Pianta S, Schauer R, Schwartz-Albiez R, Borsani G, Bresciani R, and Monti E
- Subjects
- Acetylesterase chemistry, Acetylesterase genetics, Amino Acid Sequence, Animals, COS Cells, Chlorocebus aethiops, Humans, Molecular Sequence Data, Phylogeny, Protein Isoforms chemistry, Protein Isoforms genetics, Protein Isoforms metabolism, Protein Structure, Tertiary, Protein Transport, Acetylesterase metabolism
- Abstract
Sialic acid acetyl esterase (SIAE) removes acetyl moieties from the hydroxyl groups in position 9 and 4 of sialic acid. Recently, a dispute has been opened on its association to autoimmunity. In order to get new insights on human SIAE biology and to clarify its seemingly contradictory molecular properties, we combined in silico characterization, phylogenetic analysis and homology modeling with cellular studies in COS7 cells. Genomic and phylogenetic analysis revealed that in most tissues only the "long" isoform, originally referred to lysosomal sialic acid esterase, is detected. Using the homology modeling approach, we predicted a model of SIAE 3D structure, which fulfills the topological features of SGNH-hydrolase family. In addition, the model and site-directed mutagenesis experiments allowed the definition of the residues involved in catalysis. SIAE transient expression revealed that the protein is glycosylated and is active in vitro as an esterase with a pH optimum corresponding to 8.4-8.5. Moreover, glycosylation influences the biological activity of the enzyme and is essential for release of SIAE into the culture medium. According to these findings, co-localization experiments demonstrated the presence of SIAE in membranous structures corresponding to endoplasmic reticulum and Golgi complex. Thus, at least in COS7 cells, SIAE behaves as a typical secreted enzyme, subjected to glycosylation and located along the classical secretory route or in the extracellular space. In these environments, the enzyme could act on 9-O-acetylated sialic acid residues, contributing to the fine-tuning of the various functions played by this acidic sugar., (© The Author 2015. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2015
- Full Text
- View/download PDF
48. Looking at human cytosolic sialidase NEU2 structural features with an interdisciplinary approach.
- Author
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Monti E, Benaglia G, Mozzi A, Fusi P, Longhi G, Gangemi F, Castiglioni E, Woody RW, Fornili SL, and Abbate S
- Subjects
- Circular Dichroism, Computer Simulation, Crystallization, Escherichia coli genetics, Escherichia coli metabolism, Humans, Models, Molecular, Neuraminidase chemistry, Neuraminidase genetics, Protein Conformation, Spectrophotometry, Infrared, Neuraminidase metabolism
- Abstract
Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations. The results show the prevalence of β-strands together with a considerable amount of α-helical structure, while in the solid state, from both previous X-ray diffraction analysis and our CD data on film samples, the content of β-strands is higher. In solution, a significant change in CD spectra occurs with an increase in temperature, related to a decrease in α-helix content and a slight increase in β-strand content. In the same range of temperatures, the enzymatic activity decreases. Although the overall structure of the protein appears to be particularly stable, molecular dynamics simulations performed at various temperatures evidence local conformational changes possibly relevant for explaining the relative lability of enzymatic activity.
- Published
- 2014
- Full Text
- View/download PDF
49. Mother to child transmission of hepatitis C virus in a province of northern Italy.
- Author
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Veronesi L, Verrotti Di Pianella C, Benassi L, Benaglia G, Affanni P, and Tanzi ML
- Subjects
- Adult, Female, Hepatitis C epidemiology, Humans, Infant, Newborn, Italy epidemiology, Hepatitis C transmission, Hepatitis C Antibodies analysis, Infectious Disease Transmission, Vertical
- Abstract
Introduction: Study reports of mother to child transmission of hepatitis C virus (HCV) have shown transmission rates ranging from 3 to 37%, according to maternal viremia and HIV-1 coinfection. The present study evaluated the prevalence of the HCV infection in the general population and the incidence of vertical transmission, from women who delivered in the Obstetric Clinic of the Hospital of Parma from January 1st 1996 to 31st 2001 December., Methods: Mothers and children were tested for the presence of HCV-RNA within one week after delivery. Children were considered to be infected when they were found positive at least twice for viral RNA or antibodies were still detectable at the end of the follow-up period (18 months) in blood., Results: Out of 13,025 women, 110 (0.8%) were found positive for anti-HCV antibodies; 72 of them (65.4%) were HCV-RNA positive. All 110 children were positive for anti-HCV antibodies in the first blood sample (time 0); 8 of them were HCV-RNA positive. Three children were still viremic at the end of the follow-up whereas 5 showed a clearance. No significant differences were found between viremic and nonviremic children with respect to gestational week, maternal alanine aminotransferase (ALT) levels and newborns weight at birth., Conclusion: This investigation shows that vertical transmission may occur in a general obstetric population despite a low prevalence of HCV-positive subjects.
- Published
- 2007
50. In vivo gene therapy of metachromatic leukodystrophy by lentiviral vectors: correction of neuropathology and protection against learning impairments in affected mice.
- Author
-
Consiglio A, Quattrini A, Martino S, Bensadoun JC, Dolcetta D, Trojani A, Benaglia G, Marchesini S, Cestari V, Oliverio A, Bordignon C, and Naldini L
- Subjects
- Animals, Brain enzymology, Brain metabolism, Brain pathology, Cerebroside-Sulfatase genetics, Cerebroside-Sulfatase metabolism, Humans, Learning Disabilities etiology, Leukodystrophy, Metachromatic complications, Leukodystrophy, Metachromatic pathology, Lipid Metabolism, Mice, Genetic Therapy, Genetic Vectors, Learning Disabilities prevention & control, Lentivirus genetics, Leukodystrophy, Metachromatic therapy
- Abstract
Metachromatic leukodystrophy (MLD) is a lipidosis caused by deficiency of arylsulfatase A (ARSA). Although the genetics of MLD are known, its pathophysiology is not understood. The disease leads to progressive demyelination and early death and no effective treatment is available. We used lentiviral vectors to deliver a functional ARSA gene (human ARSA) into the brain of adult mice with germ-line inactivation of the mouse gene encoding ARSA, As2. We report sustained expression of active enzyme throughout a large portion of the brain, with long-term protection from development of neuropathology and hippocampal-related learning impairments. We show that selective degeneration of hippocampal neurons is a central step in disease pathogenesis, and provide evidence that in vivo transfer of ARSA by lentiviral vectors reverts the disease phenotype in all investigated areas. Therefore, in vivo gene therapy offers a unique option for MLD and other storage diseases affecting the central nervous system.
- Published
- 2001
- Full Text
- View/download PDF
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