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9 results on '"Benaoudia, Sacha"'

4. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11

Author

Lagrange, Brice, Benaoudia, Sacha, Wallet, Pierre, Magnotti, Flora, Provost, Angelina, Michal, Fanny, Martin, Amandine, Di Lorenzo, Flaviana, Py, Bénédicte F., Molinaro, Antonio, Henry, Thomas, Inflammasome, Infections bactériennes et autoinflammation, Inflammasome, Bacterial Infections and Autoinflammation (I2BA), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Chemical Sciences, University of Naples Federico II, Napoli, Italy, Inflammasome NLRP3 – NLRP3 Inflammasome, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Naples Federico II = Università degli studi di Napoli Federico II, Lagrange, Brice, Benaoudia, Sacha, Wallet, Pierre, Magnotti, Flora, Provost, Angelina, Michal, Fanny, Martin, Amandine, Di Lorenzo, Flaviana, Py, Bénédicte F., Molinaro, Antonio, and Henry, Thomas

Subjects
Lipopolysaccharides, Macrophage, Inflammasomes, Science, Knockout, Acylation, Cells, Lipopolysaccharide, NLR Family, Inbred C57BL, Article, Inflammasome, Physics and Astronomy (all), Mice, Cytosol, Species Specificity, NLR Family, Pyrin Domain-Containing 3 Protein, Animals, Humans, Francisella, lcsh:Science, Cells, Cultured, Mice, Knockout, Biochemistry, Genetics and Molecular Biology (all), Cultured, Animal, Macrophages, Chemistry (all), Initiator, U937 Cells, Caspase, Pyrin Domain-Containing 3 Protein, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Caspases, Initiator, Mice, Inbred C57BL, Caspases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.IMM]Life Sciences [q-bio]/Immunology, lcsh:Q, lipids (amino acids, peptides, and proteins), RNA Interference, U937 Cell, Human
Abstract

Caspase-4/5 in humans and caspase-11 in mice bind hexa-acylated lipid A, the lipid moeity of lipopolysaccharide (LPS), to induce the activation of non-canonical inflammasome. Pathogens such as Francisella novicida express an under-acylated lipid A and escape caspase-11 recognition in mice. Here, we show that caspase-4 drives inflammasome responses to F. novicida infection in human macrophages. Caspase-4 triggers F. novicida-mediated, gasdermin D-dependent pyroptosis and activates the NLRP3 inflammasome. Inflammasome activation could be recapitulated by transfection of under-acylated LPS from different bacterial species or synthetic tetra-acylated lipid A into cytosol of human macrophage. Our results indicate functional differences between human caspase-4 and murine caspase-11. We further establish that human Guanylate-binding proteins promote inflammasome responses to under-acylated LPS. Altogether, our data demonstrate a broader reactivity of caspase-4 to under-acylated LPS than caspase-11, which may have important clinical implications for management of sepsis., Lipid A from some bacteria is sensed differently by humans and mice for the activation of the inflammasomes and inflammatory responses, but the mechanisms are not clear. Here, the authors show that murine caspase-11 and human caspase-4/5 contribute to this differential response via their distinct recognition of under-acylated lipid A.

Published
2018

5. A genome‐wide screen identifies IRF2 as a key regulator of caspase‐4 in human cells

Author

Benaoudia, Sacha, primary, Martin, Amandine, additional, Puig Gamez, Marta, additional, Gay, Gabrielle, additional, Lagrange, Brice, additional, Cornut, Maxence, additional, Krasnykov, Kyrylo, additional, Claude, Jean‐Baptiste, additional, Bourgeois, Cyril F, additional, Hughes, Sandrine, additional, Gillet, Benjamin, additional, Allatif, Omran, additional, Corbin, Antoine, additional, Ricci, Romeo, additional, and Henry, Thomas, additional

Published
2019
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7. Bclxl Prevents Progressive Exhaustion in BCMA CAR T Cells with Maintenance of High TCF1 Expressing T Cells

Author

Poorebrahim, Mansour, Lee, Holly, Benaoudia, Sacha, Maity, Ranjan, Ahn, Sungwoo, Leblay, Noémie, Barakat, Elie, Tilmont, Remi, Mahoney, Douglas, Boise, Lawrence H., Neri, Paola, and Bahlis, Nizar J

Abstract

Although BCMA targeted CAR T cell therapies are highly effective in the treatment of multiple myeloma (MM), patients with high disease burden have not fully benefited from this therapy with shorter disease remissions despite initial responses. It is postulated that high disease burden results in chronic antigenic stimulation of CAR T cells with the induction of exhaustion-associated gene signatures and CAR T cells contraction, limiting their in vivopotency. We have previously reported that BCL2L1(gene encoding for BCLxL) armoring of BCMA CAR T cells enhanced CAR T cells functional fitness and improved proliferation and cytotoxicity after chronic antigenic stimulation in vitro, as well as superior tumor clearance in xenograft models of MM. In the current study we aimed to define the molecular mechanisms that mediates BCL2L1-armored CAR T cell and examine the effect of BCLxL variants on CAR T cells fitness.

Published
2023
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8. Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures

Author

Essaidi-Laziosi, Manel, primary, Brito, Francisco, additional, Benaoudia, Sacha, additional, Royston, Léna, additional, Cagno, Valeria, additional, Fernandes-Rocha, Mélanie, additional, Piuz, Isabelle, additional, Zdobnov, Evgeny, additional, Huang, Song, additional, Constant, Samuel, additional, Boldi, Marc-Olivier, additional, Kaiser, Laurent, additional, and Tapparel, Caroline, additional

Published
2018
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9. IFN-γ extends the immune functions of Guanylate Binding Proteins to inflammasome-independent antibacterial activities during Francisella novicida infection

Author

Wallet, Pierre, primary, Benaoudia, Sacha, additional, Mosnier, Amandine, additional, Lagrange, Brice, additional, Martin, Amandine, additional, Lindgren, Helena, additional, Golovliov, Igor, additional, Michal, Fanny, additional, Basso, Pauline, additional, Djebali, Sophia, additional, Provost, Angelina, additional, Allatif, Omran, additional, Meunier, Etienne, additional, Broz, Petr, additional, Yamamoto, Masahiro, additional, Py, Bénédicte F., additional, Faudry, Eric, additional, Sjöstedt, Anders, additional, and Henry, Thomas, additional

Published
2017
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