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6. Improved Adherence to ART in Children – Orphans with AIDS Results in the Decreasing Occurrence of Tuberculosis (Research paper)

Author

A. Beresova, Benca J, B. Durcova, Bartkovjak M, P. A. Haj, S. Seta, M. Olah, Gertruda Mikolasova, Hvizdak F, H. Hoin, and J. Otrubova

Subjects
medicine.medical_specialty, Tuberculosis, Acquired immunodeficiency syndrome (AIDS), business.industry, Family medicine, Medicine, General Medicine, business, medicine.disease
Published
2018
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9. Education Harmonization in Nursing and Social Work as Response to Vulnerable Patient/Client Groups in the new Candidate Member States – Solidarity from European Union (Note)

Author

Otrubova, J., primary, Kalatova, D., additional, Murgova, A., additional, Katunska, M., additional, Zacharova, E., additional, Matulnikova, L., additional, Sramkova, M., additional, Libova, L., additional, Bydzovsky, J., additional, Jankechova, M., additional, Kozon, V., additional, Olah, M., additional, Konosova, H., additional, Karvaj, M., additional, Benca, J., additional, Cauda, R., additional, Sabo, A., additional, and Marks, P., additional

Published
2019
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14. Rehabilitation and Nursing Homes with Elderly and Homeless Population, Lessons not only for Physiotherapy but also for Epidemiology?

Author

Subramanian, S., primary, Belovicova, M., additional, Vansac, P., additional, Palun, M., additional, Radkova, L., additional, Otrubova, J., additional, Vlcek, R., additional, Benca, J., additional, Olah, M., additional, Matulnikova, L., additional, Sramkova, M., additional, Cmorej, P., additional, Krcmery, V., additional, and Shahum, A., additional

Published
2018
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15. Joint, Soft Tissue and Wound Infections in Physiotherapy Patients from a Mobile Health Care Unit in a Refugee Camp in Northern Bosnia

Author

Naddour, A., primary, Jackulikova, M., additional, Drgova, J., additional, Suvada, J., additional, Kmit, I., additional, Hochman, R., additional, Krcmery, V., additional, Liskova, A., additional, Benca, J., additional, Cauda, R., additional, Palenikova, M., additional, Olah, M., additional, Belovicova, M., additional, and Herdics, G., additional

Published
2018
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17. Demand on Non-Medical Health Professions Training: Adaptation to New Challenges of the Aging Populations (letter)

Author

Hardy, M., primary, Vansac, P., additional, Benca, J., additional, Palun, M., additional, Gallova, A., additional, Susta, M., additional, Otrubova, J., additional, Jankechova, M., additional, Matulnikova, L., additional, Subramanian, S., additional, Sramkova, M., additional, Cmorej, P., additional, West, D., additional, and Kimuli, D., additional

Published
2018
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19. Abstract P2-01-05: Comprehensive analysis of genomic alterations in tumor tissue associated with presence of various subpopulations of circulating tumor cells (CTCs) in primary breast cancer

Author

Mego, M, primary, Tokar, T, additional, Minarik, G, additional, Hajduk, M, additional, Karaba, M, additional, Benca, J, additional, Sedlackova, T, additional, Repiska, G, additional, Krasnicanova, L, additional, Macuch, J, additional, Sieberova, G, additional, Pindak, D, additional, Cristofanilli, M, additional, Reuben, JM, additional, Jurisica, I, additional, and Mardiak, J, additional

Published
2018
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20. Quality of Life, Risk Factors and Mortality in Children with HIV/AIDS on 2nd Line Treatment, Slow Progressors and Late Presenters in Cambodian Orphanage

Author

Shahum, A., primary, Kalavska, A., additional, Chabadova, M., additional, Kalavsky, E., additional, Benca, J., additional, Mrazova, M., additional, Polonova, J., additional, Tomanek, P., additional, Obtulovic, M., additional, Dobrodenkova, S., additional, Sladeckova, V., additional, Bujdova, N., additional, Hrindova, T., additional, Mikolasova, G., additional, Sladicekova, R., additional, Horak, P., additional, Dudova, Z., additional, Adamcova, J., additional, Spanik, S., additional, Belovicova, M., additional, Bartosovic, I., additional, Laca, S., additional, Bak, T., additional, Gallova, A., additional, Olah, M., additional, and Krcmery, V., additional

Published
2017
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21. Longitudinal survey of ATB resistance reversibility in children with AIDS of genocide survivors in Cambodia

Author

Shahum, A., primary, Benca, J., additional, Liskova, A., additional, Krcmery, V., additional, Mamova, A., additional, Hruska, J., additional, Zidisinova, M., additional, Michalikova, L., additional, Galbavy, A., additional, Balogova, E., additional, Chabadova, M., additional, Sladeckova, V., additional, Kafkova, J., additional, Putekova, S., additional, Kalavska, A., additional, Kalavsky, E., additional, Jankechova, M., additional, Kalatova, D., additional, Tkacova, L., additional, Murgova, A., additional, Lachytova, L., additional, and Cheng Hoin, H.L.H., additional

Published
2016
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22. Six month follow up in communicable versus non-communicable diseases in an Iraqi refugee cam

Author

Dudova, Z., primary, Trilisinskaya1, Y., additional, Jackuliakova, T., additional, Jankechova, M., additional, Sasvary, F., additional, Matel, A., additional, Takac, B., additional, Krizanova, A., additional, Otrubova, J., additional, Komlosi, M., additional, Benca, J., additional, Krcmery, V., additional, Kmit, I., additional, Kuranova, Z., additional, Mamova, A., additional, Zidisinova, M., additional, Petrik, A., additional, and Alhaj Ali, I.K., additional

Published
2016
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23. Experience with Migrants on Balkan Route from the Field Hospital on Slovenian-Croatian Border

Author

Bydzovsky, J., primary, Chrasc, S., additional, Peciar, A., additional, Benca, J., additional, Popelova, M., additional, Sasvary, F., additional, Matel, A., additional, Cernicky, M., additional, Kalatova, D., additional, Nevolna, Z., additional, Kafkova, J., additional, Zollerova, C., additional, Kolibab, M., additional, Putekova, S., additional, Martinkova, J., additional, Mikolasova, G., additional, and Komlosi, M., additional

Published
2016
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24. Antibiotic susceptibility of respiratory isolates among HIV-positive children in Kyrgyzstan is higher compared to those Cambodia: Is outpatients status important

Author

Suvada, J., primary, Liskova, A., additional, Sladeckova, V., additional, Benca, J., additional, Gornerova, L., additional, Mikolasova, G., additional, Kafkova, J., additional, Vujcikova, J., additional, Kalavska, A., additional, Minarik, P., additional, Krcmery, V., additional, Kulkova, N., additional, and Stalbek, M., additional

Published
2016
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26. CXCR4-SDF-1 interaction potentially mediates trafficking of circulating tumor cells in primary breast cancer

Author

Mego, M., primary, Cholujova, D., additional, Minarik, G., additional, Sedlackova, T., additional, Gronesova, P., additional, Karaba, M., additional, Benca, J., additional, Cingelova, S., additional, Cierna, Z., additional, Manasova, D., additional, Pindak, D., additional, Sufliarsky, J., additional, Cristofanilli, M., additional, Reuben, J. M., additional, and Mardiak, J., additional

Published
2016
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27. Abstract P2-02-04: Distinct clinical and biological values of subpopulations of circulating tumor cells (CTCs) in primary breast cancer

Author

Mego, M, primary, Jurisova, S, additional, Karaba, M, additional, Minarik, G, additional, Benca, J, additional, Sedlackova, T, additional, Manasova, D, additional, Malejcikova, M, additional, Sieberova, G, additional, Macuch, J, additional, Gronesova, P, additional, Sufliarsky, J, additional, Pindak, D, additional, Cristofanilli, M, additional, Reuben, JN, additional, and Mardiak, J, additional

Published
2016
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28. Relationship between circulating tumor cells and epithelial to mesenchymal transition in early breast cancer

Author

Mego, M., primary, Cierna, Z., additional, Janega, P., additional, Karaba, M., additional, Minarik, G., additional, Benca, J., additional, Sedlácková, T., additional, Sieberova, G., additional, Gronesova, P., additional, Manasova, D., additional, Pindak, D., additional, Sufliarsky, J., additional, Danihel, L., additional, Reuben, JM, additional, and Mardiak, J., additional

Published
2015
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29. Bacterial meningitis after sinusitis and otitis media: ear, nose, throat infections are still the commonest risk factors for the community acquired meningitis

Author

Lesnakova, A., Holeckova, K., Kolenova, A., Streharova, A., Kisac, P., Beno, P., Kalavsky, E., Sramka, M., Ondrusova, A., Benca, J., Seckova, S., Sladeckova, V., Bartkovjak, M., Bukovinova, P., Hvizdak, F., Lengyel, P., Sabo, I., Rudinsky, B., Bauer, F., Luzica, R., Bielova, M., Karvaj, M., Huttova, M., Wiczmandyova, D., Svabova, V., Findova, L., Kutna, K., Deadline, J., Diana, E., and Kiwou, M.

Subjects
Community-Acquired Infections, Diabetes Complications, Otitis Media, Risk Factors, Outcome Assessment, Health Care, Humans, Wounds and Injuries, Sinusitis, Alcohol-Related Disorders, Anti-Bacterial Agents, Meningitis, Bacterial
Abstract

We investigated how many cases of bacterial meningitis in our national survey were associated with sinusitis or otitis media. Among 372 cases of bacterial meningitis within our nationwide 17 years survey, 201 cases were community acquired (CBM) and in 40 (20%) otitis media or sinusitis acuta/chronica were reported 1-5 weeks before onset of CBM. Diabetes mellitus (20% vs. 7.5%, p=0.01), alcohol abuse (35% vs. 15.4%, p=0.003) and trauma (30% vs. 14.9%, p=0.02) were significantly associated with CBM after ENT infections. Concerning etiology, CBM after sinusitis/otitis was insignificantly associated with pneumococcal etiology (50% vs. 33.8 %, NS) and significantly associated with other (L. monocytogenes, Str. agalactiae) bacterial agents (9.9 % vs. 25 %, p=0.008) . However those significant differences for new ENT related CBM had no impact on mortality (12.4 % vs. 5%, NS), failure after initial antibiotics (10 % vs. 9.5%, NS) and neurologic sequellae (12.5 % vs. 15.4 %, NS).

Published
2007

30. Bacterial meningitis after craniocerebral trauma in the community

Author

Holeckova, K., Kolenova, A., Lesnakova, A., Steno, J., Streharova, A., Kisac, P., Beno, P., Kalavsky, E., Sramka, M., Ondrusova, A., Benca, J., Seckova, S., Sladeckova, V., Bartkovjak, M., Bukovinova, P., Hvizdak, P., Lengyel, P., Svabova, V., Findova, L., Kutna, K., Deadline, J., Johnson, J., Namulanda, V., Juma, O., Okoth, V., Kovac, M., Rudinsky, B., Bauer, F., Karvaj, M., Sabo, I., Huttova, M., Kenderessy, P., Nosko, P., Paulikova, T., Rusnak, R., Povinsky, J., and Galla, S.

Subjects
Cohort Studies, Community-Acquired Infections, Bacteria, Risk Factors, Outcome Assessment, Health Care, Craniocerebral Trauma, Humans, Brain Damage, Chronic, Alcohol-Related Disorders, Anti-Bacterial Agents, Meningitis, Bacterial
Abstract

Craniocerebral trauma is one of major risk factors for development of meningitis. We reviewed 30 cases of bacterial meningitis occurring in community after craniocerebral trauma. Alcohol abuse was significant risk factor occurring in trauma patients with meningitis present in 50% in our cohort (p=0.0001). The most common pathogen in posttraumatic meningitis was Str. pneumoniae (90% vs. 33.8%, p=0.0001). However mortality was very low, only 5% probably because of early diagnosis and treatment of patients at risk for bacterial meningitis but neurologic sequellea were significantly more common (p=0.00001) in patients after craniocerebral trauma.

Published
2007

31. Pneumococcal meningitis in community is frequent after craniocerebral trauma and in alcohol abusers

Author

Benca, J., Lesnakova, A., Holeckova, K., Ondrusova, A., Wiczmandyova, D., Sladecko, V., Hvizdak, F., Bartkovjak, M., Seckova, S., Taziarova, M., Huttova, M., Bielova, M., Luzica, R., Karvaj, M., Kovac, M., Bauer, F., Sabo, I., Svabova, V., Findova, L., Bukovinova, P., and Shahum, A.

Subjects
Community-Acquired Infections, Meningitis, Pneumococcal, Risk Factors, Outcome Assessment, Health Care, Craniocerebral Trauma, Humans, Meningitis, Macrolides, Penicillins, Alcohol-Related Disorders, Anti-Bacterial Agents
Abstract

Aim of this short communication was to assess risk factors and outcome of community acquired pneumococcal meningitis and compare it to all cases of community acquired meningitis. Univariate analysis was used for comparison of 68 pneumococcal to 201 CBM within a Slovak nationwide database of CBM. Significant risk factors for pneumococcal meningitis were previous craniocerebral trauma within 7 days (39.7% vs. 14.9%, p=0.00002), splenectomy (10.3% vs. 3.5%, p=0.03) and alcohol abuse (36.8% vs. 15.4%, p=0.0001). Concerning outcome, mortality was similar (8,8% and 12,4%, NS), proportion of those with neurologic sequellae after CBM due to Str. pneumoniae was insignificantly higher (20.6% vs. 15.4%, NS) in comparison to all CBM. All but 2 strains Str. pneumoniae were susceptible to penicillin and macrolides (3.3% resistance).

Published
2007

32. Predictors of inferior outcome in community acquired bacterial meningitis

Author

Streharova, A., Krcmery, V., Kisac, P., Kalavsky, E., Holeckova, K., Lesnakova, A., Luzinsky, L., Adamkovicova, E., Pavlikova, Z., Spilakova, N., Kacunova, B., Dovalova, V., Wiczmandyova, O., Spanik, S., Liskova, A., Chovancova, D., Kovac, M., Ondrusova, A., Bauer, F., Benca, J., Rudinsky, B., Sramka, M., Kralova, J., Krsakova, A., Krumpolcova, M., Findova, L., Svabova, V., Sladeckova, V., Seckova, S., Saniova, J., Pavlicova, B., Taziarova, M., Bukovinova, P., Kolenova, A., Horvathova, D., Hvizdak, F., Luzica, R., Rolnikova, B., Bocakova, A., Grey, E., Bielova, M., Huttova, E., Sabo, I., and Jalili, N.

Subjects
Community-Acquired Infections, Alcoholism, Slovakia, Chi-Square Distribution, Risk Factors, Brain Injuries, Diabetes Mellitus, Humans, Brain Damage, Chronic, Treatment Failure, Gram-Negative Bacterial Infections, Meningitis, Bacterial
Abstract

The aim of this study was to assess mortality and sequellae within cases from Nationwide survey of community acquired meningitis and identify risk factors for inferior outcome. Risk factors such as underlying disease (diabetes mellitus, cancer, trauma, neonatal age, splenectomy, alcoholism, sepsis, other infections), etiology, clinical symptoms and outcome (death, improvement and cured after modifications of ATB therapy, cured without change of therapy, cured with neurologic sequellae) were recorded and analysed with univariate analysis (chi2 or t test for trends, CDC Atlanta 2004). Analysing risk factors for inferior outcome (death or cured with neurologic sequellae), we compared patients who died or survived with neurologic sequellae to all patients with community acquired bacterial meningitis. Univariate analysis showed that trauma (p0.05), alcohol abuse (p0.05), diabetes, S. aureus (p0.05) and gram-negative etiology (A. baumannii, Ps. aeruginosa or Enterobacteriaceae) (36% vs. 11,9%, p0.05) were predicting inferior outcome. Analysing risk factors for treatment failure (death or failed but cured after change of antibiotic treatment) prior sepsis (34.1% vs. 13.9%, p0.01) and gram-negative etiology (25% vs. 11.9%, p0.02) were statistically significant predictors of treatment failure. Neisseria meningitis had less failures (p0.05). Concerning infection associated mortality again diabetes mellitus (p0.05), alcoholism (p0.05) staphylococcal and gram-negative etiology (p0.05) were significant predictors of death. N. meningitis had surprisingly less treatment failures (appropriate and rapid initial therapy). Neurologic sequellae were more common in patients with alcohol abuse (p0.05), craniocerbral trauma (p0.05) and less common in meningitis with pneumococcal etiology (p0.05).

Published
2007

33. Neuroinfections due to Staphylococcus aureus: an emerging pathogen also in community acquired meningitis

Author

Benca, J., Lesnakova, A., Holeckova, K., Ondrusova, A., Wiczmandyova, D., Sladeckova, V., Hvizdak, F., Bartkovjak, M., Seckova, S., Taziarova, M., Huttova, M., Bielova, M., Luzica, R., Karvaj, M., Kovac, M., Bauer, F., Sabo, I., Svabova, V., Findova, L., Bukovinova, P., and Shahum, A.

Subjects
Community-Acquired Infections, Diabetes Complications, Staphylococcus aureus, Outcome Assessment, Health Care, Diabetes Mellitus, Humans, Staphylococcal Infections, Anti-Bacterial Agents, Meningitis, Bacterial, Oxacillin
Published
2007

35. Evaluation of protein expression and DNA methylation profiles detected by pyrosequencing in invasive breast cancer

Author

Zmetakova, I., primary, Danihel, L., additional, Smolkova, B., additional, Mego, M., additional, Kajabova, V., additional, Krivulcik, T., additional, Rusnak, I., additional, Rychly, B., additional, Danis, D., additional, Repiska, V., additional, Blasko, P., additional, Karaba, M., additional, Benca, J., additional, Pechan, J., additional, and Fridrichova, I., additional

Published
2014
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36. Abstract P1-04-02: Correlation between blood markers of hemostasis and circulating tumor cells (CTCs) in early breast cancer patients

Author

Mego, M, primary, Karaba, M, additional, Minarik, G, additional, Benca, J, additional, Sedlackova, T, additional, Tothova, L, additional, Vlkova, B, additional, Cierna, Z, additional, Janega, P, additional, Manasova, D, additional, Luha, J, additional, Gronesova, P, additional, Pechan, J, additional, Fridrichova, I, additional, Celec, P, additional, Reuben, JM, additional, Cristofanilli, M, additional, and Mardiak, J, additional

Published
2013
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41. P776 Clinical symptoms, treatment and outcome of Highlands malaria in Eldoret (2,400 m a.s.l.) and comparison to malaria in hyper-immune population in endemic region of South Sudan

Author

Farkasova, A., primary, Seckova, S., additional, Nagyova, Z., additional, Kolenova, A., additional, Olejcekova, P., additional, Sabo, I., additional, Gabrhelova, L., additional, Krcmery, V., additional, Kisac, P., additional, Kalavsky, E., additional, Kiwou, M., additional, Taziarova, M., additional, Bukovinova, P., additional, Ocenas, M., additional, Kralova, J., additional, Kutna, K., additional, Mykyta, J., additional, Velganova, M., additional, Augustinova, A., additional, Pivarnik, M., additional, Duris, M., additional, Uhercik, L., additional, Benca, J., additional, and Pastekova, T., additional

Published
2007
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42. P1979 Risk factors for voriconazole resistance in non/albicans candida in HIV-positive children

Author

Shahum, A., primary, Krcmery, V., additional, Liskova, A., additional, Kisac, P., additional, Hricak, V., additional, Karvaj, M., additional, Augustinova, A., additional, Kalavsky, E., additional, Benca, J., additional, Seckova, S., additional, Holeckova, K., additional, Havlikova, Z., additional, Sladeckova, V., additional, Horvathova, D., additional, and Havrilova, H., additional

Published
2007
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44. Resistance in Uropathogens among HIV-Positive Kenyan and Cambodian Children in Comparison to an HIV-Negative Population in South Sudan

Author

Augustin, A., primary, Shahum, A., additional, Taziar, M., additional, Benca, J., additional, Koleno, B., additional, Bukovino, P., additional, Holecko, K., additional, Secko, S., additional, Kral, J., additional, Duris, M., additional, Ocenas, M., additional, Horvath, D., additional, Sladecko, V., additional, Kniezo, Z., additional, Bartkovjak, M., additional, Havlik, Z., additional, Kalavsky, E., additional, Liska, A., additional, and Krcmery, V., additional

Published
2007
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45. Comparison of etiology and resistance in respiratory isolates of AIDS patients vs. non-AIDS patients in South Sudan and Kenya

Author

Sladeckova, V., primary, Fabianova, L., additional, Kolenova, A., additional, Horvathova, D., additional, Kniezova, Z., additional, Doczeova, A., additional, Taziarova, M., additional, Benca, J., additional, Bartkoviak, M., additional, Seckova, S., additional, Liskova, A., additional, Beno, P., additional, Babelova, O., additional, and Krcmery, V., additional

Published
2006
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47. Pilot study of gene mutations associated with Lynch syndrome in Slovak patients with breast cancer.

Author

Krasničanová L, Saade R, Priščáková P, Gbelcová H, Kaľavská K, Karaba M, Benca J, Mego M, and Repiská V

Subjects
Female, Humans, Pilot Projects, Genetic Predisposition to Disease, Mismatch Repair Endonuclease PMS2 genetics, Slovakia, Neoplasm Recurrence, Local, Mutation, Germ-Line Mutation, DNA Mismatch Repair, Breast Neoplasms genetics, Colorectal Neoplasms, Hereditary Nonpolyposis genetics, Colorectal Neoplasms, Hereditary Nonpolyposis diagnosis, Colorectal Neoplasms, Hereditary Nonpolyposis pathology
Abstract

Background: Lynch syndrome (LS) is an autosomal dominant inherited disorder which causes an increased risk of cancer, especially colorectal and endometrial carcinomas. Recent studies have shown an association between LS and breast cancer as well. The aim of our study is to highlight the possible presence of mutations in genes associated with LS in patients with breast cancer and the need to include the examination of Lynch-associated genes in patients with a family history of breast cancer as well as in patients with recurrent breast cancer, as well as with the occurrence of other Lynch-associated cancer., Materials and Methods: We analyzed tumor tissue samples from 78 patients with primary breast cancer. Our samples were tested with a gene panel associated with the risk of developing breast cancer, while in our study we focused primarily on the occurrence of mutations in mismatch-repair genes. DNA isolated from tumor tissue was sequenced using next generation sequencing (NGS) and analyzed using the Ingenuity Variant Analysis tool. To confirm the germline mutation, we examined the patient's blood sample using NGS sequencing., Results: As a result of our analysis, we managed to identify a mutation in the PMS2 gene in one patient's breast tumor tissue. The presence of this mutation indicates that the resulting cancer may be a consequence of LS. As for pathogenicity, this was probably a pathogenic variant, as we detected deletions in the exon region, which led to frameshift mutation. Moreover, we also identified single-nucleotide pathogenic variants in the TP53 and PIK3CA genes. To definitively establish the diagnosis of LS in the patient, we examined a blood sample, where we also identified a mutation of the PMS2 gene., Conclusion: LS is underdiagnosed in many Lynch-associated cancers. However, in the case of a familial occurrence of breast cancer and other Lynch-associated genes, it is important to think about a possible diagnosis of LS and, if the patient meets the diagnostic criteria, to carry out a genetic examination of Lynch-associated genes.

Published
2023
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48. Vitamin D and circulating tumor cells in primary breast cancer.

Author

Mego M, Vlkova B, Minarik G, Cierna Z, Karaba M, Benca J, Sedlackova T, Cholujova D, Gronesova P, Kalavska K, Pindak D, Mardiak J, and Celec P

Abstract

Background: Circulating tumor cells (CTCs) contribute to the metastatic cascade and represent an independent survival predictor in breast cancer (BC) patients. Vitamin D has pleiotropic effects, and its low concentrations are associated with breast cancer and metastasis. The aim of this study was to assess plasma vitamin D in primary BC patients in relation to CTCs., Methods: This study included 91 non-metastatic BC patients (stage I-III) and 24 healthy donors. Blood samples for the analyses were drawn at the time of surgery. CTCs were assessed using a quantitative RT-PCR assay for expression of epithelial ( CK19 ) or epithelial-to-mesenchymal transition (EMT) genes ( TWIST1 , SNAIL1 , SLUG , and ZEB1 ). Total 25-OH vitamin D was measured in plasma using ELISA. Plasma cytokines and angiogenic factors were measured by enzyme-linked immunoassay., Results: CTCs were detected in 30 (33%) patients. Patients with detectable CTCs in peripheral blood had significantly lower vitamin D concentrations in comparison to patients without detectable CTCs ((mean ± SD) 8.50 ± 3.89 µg/L for CTC-positive vs 9.69 ± 3.49 µg/L for CTC-negative patients, p = 0.03). The mean ( ± SD) vitamin D plasma level was 9.3 ± 3.65 µg/L for breast cancer patients compared to 18.6 ± 6.8 for healthy donors (p < 0.000001). There was no association between plasma vitamin D and other patient/tumor characteristics. Plasma vitamin D levels are inversely correlated with plasma TGF-β1, TGF-β2, IL β, IL-5, and eotaxin (all p < 0.05). Patients with vitamin D above the median had a better overall survival (hazard ratio (HR) = 0.36, 95% CI 0.16-0.80, p = 0.017), and combined analysis showed the best survival for CTC-negative patients with vitamin D levels above the median as compared to patients with opposite characteristics (HR = 0.18, 95% CI 0.05-0.63, p = 0.004)., Conclusions: Low vitamin D could be a consequence and hence a biomarker of a more invasive disease. Alternatively, vitamin D could be associated with survival because of its role in tumor dissemination. Whether its supplementation affects the metastatic cascade should be tested in animal experiments and interventional studies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Mego, Vlkova, Minarik, Cierna, Karaba, Benca, Sedlackova, Cholujova, Gronesova, Kalavska, Pindak, Mardiak and Celec.)

Published
2022
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49. Uncovering Microbial Composition in Human Breast Cancer Primary Tumour Tissue Using Transcriptomic RNA-seq.

Author

Hadzega D, Minarik G, Karaba M, Kalavska K, Benca J, Ciernikova S, Sedlackova T, Nemcova P, Bohac M, Pindak D, Klucar L, and Mego M

Subjects
Case-Control Studies, Female, Humans, Neoplastic Cells, Circulating, Transcriptome, Breast microbiology, Breast Neoplasms microbiology, Microbiota
Abstract

Recent research studies are showing breast tissues as a place where various species of microorganisms can thrive and cannot be considered sterile, as previously thought. We analysed the microbial composition of primary tumour tissue and normal breast tissue and found differences between them and between multiple breast cancer phenotypes. We sequenced the transcriptome of breast tumours and normal tissues (from cancer-free women) of 23 individuals from Slovakia and used bioinformatics tools to uncover differences in the microbial composition of tissues. To analyse our RNA-seq data (rRNA depleted), we used and tested Kraken2 and Metaphlan3 tools. Kraken2 has shown higher reliability for our data. Additionally, we analysed 91 samples obtained from SRA database, originated in China and submitted by Sichuan University. In breast tissue, the most enriched group were Proteobacteria , then Firmicutes and Actinobacteria for both datasets, in Slovak samples also Bacteroides , while in Chinese samples Cyanobacteria were more frequent. We have observed changes in the microbiome between cancerous and healthy tissues and also different phenotypes of diseases, based on the presence of circulating tumour cells and few other markers.

Published
2021
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50. Prognostic role of matrix metalloproteinase 9 in early breast cancer.

Author

Kalavska K, Cierna Z, Karaba M, Minarik G, Benca J, Sedlackova T, Kolekova D, Mrvova I, Pindak D, Mardiak J, and Mego M

Abstract

MMP9 is involved in extracellular matrix degradation during various physiological and pathological conditions, including tumorigenesis. The present study aimed to assess the prognostic role of intratumoral MMP9 and to determine its association with circulating tumor cells (CTCs) in patients with early breast cancer. A total of 318 patients with primary breast cancer (PBC) were enrolled into the present study. Specimens were subjected to immunohistochemistry analysis, using the MMP9 monoclonal antibody. MMP9 expression was scored using a weighted histoscore (WH). The results demonstrated that the mean WH ± SEM for MMP9 expression was significantly higher in breast tumor cells compared with tumor associated stromas (132.0±5.2 vs. 50.8±3.7; P<0.00001). Furthermore, a positive association was observed between MMP9 expression, the hormone positive status and proliferation index of analysed breast cancer tumour cells. Notably, the prognostic role of MMP9 was not observed in tumor cells [hazard ratio (HR) =0.96; 95% confidence interval (CI), 0.58-1.59; P=0.864] or tumor associated stroma (HR=1.29; 95% CI, 0.60-2.78; P=0.547). Subgroup analysis demonstrated that patients that were HR negative or triple negative, with low MMP9 expression in tumor cells and stroma had a significantly improved disease-free survival than patients with high MMP9 expression. Taken together, the results of the present study demonstrated that high MMP9 expression in PBC was associated with favorable tumor characteristics. However, the prognostic value of MMP9 was limited to only the HR negative and CTC epithelial-to-mesenchymal transition positive subgroups. Thus, analyzing MMP9 tumor expression may help identify patients with increased risk of disease recurrence in these subgroups., (Copyright: © Kalavska et al.)

Published
2021
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