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Abstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank®. The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species.

A flora entérica dos psitacídeos é composta principalmente por bactérias Gram positivas. Bactérias Gram negativas, como Escherichia coli e Salmonella spp., apresentam elevado potencial patogênico, sendo consideradas indicativo de problemas de manejo, que poderão culminar em manifestação de doenças em decorrência de fatores estressantes, dietas deficientes e superlotação, combinados com alta carga bacteriana no ambiente. O objetivo deste trabalho foi avaliar a presença de Salmonella spp., Escherichia coli e os fatores de virulência dos genes iss e iutA dos isolados de E. coli. Analisou-se um total de 44 amostras provenientes de psitacídeos criados em cativeiro, sendo estas 15 fragmentos de órgãos de aves submetidas a exame de necropsia e também 29 amostras de swabs de cloaca e inglúvio de papagaios-charão (Amazona pretrei) criados em cativeiro. Nenhuma amostra foi positiva para Salmonella spp. Nas amostras de E. coli detectou-se ambos os fatores de virulência pesquisados.

Genetic characterization of wild-type measles viruses (MeVs) is a critical component of the global measles surveillance programme. Sequence data can be used to map the transmission of MeVs by allowing [...]

RESUMO: Celulite aviária é uma enfermidade de grande importância para a avicultura mundial, sendo relacionada principalmente à Escherichia coli (E. coli). Neste estudo foi comparada a susceptibilidade de duas linhagens de aves no desenvolvimento da celulite diante do desafio com diferentes concentrações de E. coli. Além disso, foi avaliada a relação dos genes iss e iutA com a patogenicidade de amostras de E. coli de diferentes origens (fecal/casos clínicos) em pintinhos e com a reprodução experimental da doença em aves de 35 dias de idade. Através da inoculação de frangos de corte (Cobb/Ross) com diferentes níveis de desafio (105 a 108 UFC/mL) de E. coli, não foram observadas diferenças significativas entre as linhagens quanto à sensibilidade à dermatite necrótica para a mesma dosagem (p≤0,05). A detecção dos genes iss e iutA demonstrou que estes estiveram presentes somente nas amostras provenientes de casos clínicos. Da mesma forma, estes isolados foram considerados de alta patogenicidade para pintinhos (>80% letalidade), levando a formação de áreas de lesão mais extensas (≥3cm2) em aves de 35 dias, quando comparado às amostras de origem fecal (p≤0,05). Ainda, as diferenças com relação ao tamanho de lesão foram constatadas também entre os isolados de mesma origem (p≤0,05). Desta forma, a linhagem não pode ser considerada um fator primordial para o desenvolvimento de dermatite necrótica em frangos. Ainda, sugere-se que os genes iss e iutA, quando presentes em conjunto ou isoladamente, poderiam ser considerados marcadores de virulência em cepas de E. coli causadoras de celulite aviária.

An increasingly common practice for policy-makers is to leverage e-participation to collect citizens’ opinions and improve their decision-making processes. This practice, however, is hindered by the large quantity of collected opinions which are often overloading and hard to value. This is referred to as information overload. As a way to mitigate this challenge for policy-makers, this article develops a prioritization framework for citizens’ ideas collected through e-participation. The framework builds on Design Science Research and is validated on a real-world case in collaboration with the European Commission. The resulting contributions are threefold. First, theoretical criteria, popularity and polarization, are developed to prioritize citizens’ proposals. Then, automated and quantitative metrics are proposed to measure these criteria. Finally, a prioritization matrix is developed to visually assess the relative priority of these citizens’ proposals.

The purpose of this work is to present an analytical method that allows to estimate in an approximate and fast way the presence of the non-resonant and ultra-fast multipactor effect in RF accelerating structures in the presence of high gradient electromagnetic fields. This single-surface multipactor regime, which has been little studied in the scientific literature, is characterised by appearing only under conditions of very strong RF electric fields (of the order of tens or hundreds of MV/m), where it is predominant over other types of single- or dual-surface resonance described in classical multipactor theory. This type of multipactor causes a rapid growth of the electron population and poses a serious drawback in the operation of RF accelerator components operating under high gradient conditions. Specifically, in dielectric-assist accelerating structures (DAA) it has been experimentally found that the presence of multipactor limits the maximum operating gradient of these components due to a significant increase in the reflected power due to the discharge, being this phenomenon the main problem to overcome. In a previous work, we found and described in detail by means of numerical simulations the presence of this non-resonant and ultra-fast multipactor regime in a DAA structure design for hadrontherapy. Here we aim to present a simple and fast method to predict the presence of this non-resonant and ultra-fast multipactor regime in RF accelerator structures with cylindrical revolution symmetry around the acceleration axis. This method is especially useful in the design stages of accelerating structures as it provides much faster results than numerical simulations of the multipactor, with quite good accuracy in a wide range of cases as shown in this paper.