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Objective The objectives were to validate a previously published equation for estimating basal endogenous losses (BEL) of crude protein (CP) in pigs fed nitrogen-free diets and to develop prediction equations for BEL of CP and amino acids (AA). Methods A total of 139 observations from 123 experiments in 117 papers that determined the BEL of CP and AA in pigs were collected. For the validation of the previous equation for the BEL of CP, 94 observations that were not used for developing the previous equation were used. All observations were used to develop novel equations for estimating BEL of CP in pigs based on the initial body weight (IBW). Results The validation study indicated that the slope for BEL of CP, representing a linear bias, was less than zero (−0.56; standard error [SE] = 0.130; p

Objective The present study aimed to measure fecal and urinary phosphorus (P) excretion from pigs and to develop prediction models for P excretion of pigs. Methods A total of 96 values for P excretions were obtained from pigs of 15 to 93 kg body weight (BW) fed 12 diets in four experiments and were used to develop the prediction models. All experimental diets contained exogenous phytase at 500 phytase units per kg. Body weight of pigs and dietary P concentrations were used as independent variables in the prediction models. Results The BW, feed intake, and P intake were positively correlated with total (fecal plus urinary) P excretions (r = 0.80, 0.91, and 0.94, respectively; p

Objective The present study aimed to study effects of a chronic feeding of deoxynivalenol (DON) on growth performance, organ weight, organ DON accumulation, and blood parameters in pigs. Methods Forty-eight castrated male pigs with a body weight of 10.4 kg (standard deviation = 1.7) were assigned to one of 2 diet groups in a randomized complete block design with 6 blocks of pens per diet and 4 pigs per pen. A corn-soybean meal-based control diet was prepared to contain a low DON concentration of 0.28 mg/kg. Corn distillers dried grains with solubles naturally contaminated with DON were used at 30.0% to prepare a contaminated diet with a high DON concentration of 1.8 mg/kg. During the 56-day experimental period, body weight and feed intake were recorded every 14 days. A pig from each pen was euthanized for the collection of organs and muscle every 14 days. Results Gain:feed in pigs fed the contaminated diet during days 14 to 28 and days 28 to 42 were less (p

Objective The objective was to investigate the effects of dietary benzoic acid (BA) on nitrogen (N) balance, urinary pH, slurry pH, and odorous compounds in feces and slurry of pigs. Methods Twelve barrows with an initial body weight of 56.2±2.6 kg were individually housed in metabolism crates. The animals were allocated to a replicated 6×4 incomplete Latin square design with 12 animals, 6 experimental diets, and 4 periods, resulting in 8 observations per treatment. The basal diet consisted mainly of corn, soybean meal, and rapeseed meal. Benzoic acid was supplemented to the basal diet at 0.5%, 1.0%, 1.5%, 2.0%, or 2.5% at the expense of corn starch. Each period consisted of a 4-day adaptation period and a 24-h collection period for slurry, followed by a 4-day collection period for feces and urine. On day 5, feces and urine were collected for 24 h and mixed to obtain slurry samples. Results The daily digested N linearly increased (p

This study measured the potential changes of the microbiota in the gastrointestinal tract and energy and nutrient digestibility by supplemental bacteriophages in pigs. Twelve castrated male pigs (initial mean body weight = 29.5 ± 2.3 kg) were surgically cannulated using T-cannula. The animals were housed individually in pens equipped with a feeder and a nipple waterer. The pigs were allotted to 1 of 3 experimental diets in a quadruplicated 3 × 2 Latin square design with 3 experimental diets, 2 periods, and 12 pigs resulting in 8 replicates per diet. The 3 diets were a control mainly based on corn and soybean meal with no antibiotics or bacteriophages, a diet containing 0.1% antibiotics, and a diet containing 0.2% bacteriophages. On day 5 of the experimental period, feces were collected and on days 6 and 7, ileal digesta were collected. Genomic DNA for bacteria were extracted from the ileal digesta and feces and the V4 region of the 16S rRNA gene was amplified. The ileal and fecal digestibility of energy, dry matter, organic matter, crude protein, and fiber was unaffected by dietary antibiotics or bacteriophages. At the phylum level, the supplemental antibiotic or bacteriophage tended to result in a higher proportion of Firmicutes (p = 0.059) and a lower proportion of Bacteroidetes (p = 0.099) in the ileal digesta samples compared with the control group with no difference between the antibiotic and bacteriophage groups. At the genus level, the supplemental antibiotic or bacteriophage tended to result in a higher proportion of Lactobacillus (p = 0.062) and a lower proportion of Bacteroides (p = 0.074) and Streptococcus (p = 0.088) in the ileal digesta compared with the control group with no difference between the antibiotic and bacteriophage groups. In the feces, supplemental antibiotics or bacteriophages reduced the proportion of Bifidobacterium compared with the control group (p = 0.029) with no difference between the antibiotic and bacteriophage groups. Overall, supplemental antibiotics and bacteriophages showed positive effect on the microbiota of in the ileal digesta without largely affecting energy or nutrient digestibility, with no differences between the antibiotic and bacteriophage groups in growing pigs.

Objective The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM) fed to pigs. Methods Six barrows with an initial body weight of 30.9±2.6 kg fitted with a T-cannula at the distal ileum were assigned to a 6×6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole AA source and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for four and half days, and the 3 diets containing an AA mixture were switched to the respective diets without AA mixture during the following two and half days. Ileal digesta were collected on days 6 and 7. Results The addition of an AA mixture during the adaptation period increased apparent ileal digestibility of Arg and Trp in corn (p

Objective The present experiment aimed to determine standardized ileal digestible (SID) lysine (Lys) requirements for pregnant sows individually housed under commercial farm conditions. Methods Two hundred multiparous sows (parity = 5.1±2.0) on day 42 of gestation were randomly allocated to five dietary treatments with a balanced parity. Experimental diets were formulated to contain 0.22%, 0.32%, 0.42%, 0.52%, and 0.62% of SID Lys for the mid-gestation period (days 42 to 76) and 0.36%, 0.46%, 0.56%, 0.66%, and 0.76% of SID Lys for the late gestation period (days 77 to 103). All indispensable amino acids except Lys were provided at 110% of their requirement estimates. Daily feed allowance per sow was determined based on the back-fat thickness and body condition score at the second pregnancy check and on day 90 of gestation. Three different statistical models were used to estimate the SID Lys requirement. Results Total born piglets alive per litter increased linearly and quadratically (p

The objective of the present research was to determine the nutrient utilization of full-fat black soldier fly larvae (Hermetia illucens; BSFL), which were processed by various drying methods, using in vitro procedures for pigs. Four sources of BSFL were prepared using various drying methods: (1) hot-air drying at 65 °C for 24 h; (2) microwave drying at 700 W for 5 min, three times; (3) freeze drying at −40 °C for 72 h; (4) infrared drying at medium infrared region (ranged from 2.0 to 6.0 μm) and at 95 °C for 12 min. In vitro ileal disappearance (IVID) of nutrients in the BSFL was measured using a procedure simulating the nutrient digestion and absorption in the stomach and small intestine of pigs. In vitro total tract disappearance (IVTTD) of nutrients in the BSFL was also measured using a procedure that simulated the total intestine of pigs. The contents of dry matter, ether extract, and crude protein in the dried-BSFL ranged from 94.6 to 96.8%, 49.2 to 52.8%, and 30.0 to 36.8%, respectively, on an as-is basis. Microwave drying resulted in a greater (p < 0.05) IVID of dry matter in BSFL, compared with the freeze drying or infrared drying method, which caused the least IVID of dry matter. Hot air-dried BSFL, microwave-dried BSFL, and freeze-dried BSFL had a greater (p < 0.05) IVID of crude protein and a greater (p < 0.05) IVTTD of dry matter and organic matter, compared with infrared-dried BSFL. In conclusion, the hot-air drying, microwave drying, and freeze drying of full-fat black soldier fly larvae resulted in fairly comparable and relatively high nutrient digestibility based on the present in vitro study for pigs. However, the infrared drying method impaired nutrient utilization of full-fat black soldier fly larvae for pigs.

The objectives of this study were to determine the apparent ileal digestibility and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in feed ingredients, compare the ileal digestibility of CP and AA between commercial crossbred pigs and mini-Jeju Island native pigs (JINP), and develop models for estimating SID of CP and AA for commercial pigs using mini-JINP data. The study involved five crossbred commercial pigs (31.5 ± 1.6 kg of body weight and 11 weeks of age; Landrace × Yorkshire) and five mini-JINP (31.0 ± 3.2 kg body weight and 20 weeks of age). The pigs were surgically equipped with a T-cannula at the end of ileum. Each pig breed was assigned to 5 dietary treatments in a 5 × 10 incomplete Latin square design with 10 periods. Four experimental diets were formulated to contain each of soybean meal, corn gluten feed, copra meal, and sesame expellers as the sole source of nitrogen. A nitrogen-free diet was also prepared to determine basal endogenous losses of CP and AA. No interaction between breed and feed ingredient was observed for the digestibility of CP and all indispensable AA. The SID of CP and all indispensable AA, except Arg, His, and Lys, did not differ between the two breeds of pigs. Prediction equations were developed for SID of CP and AA of commercial pigs using the SID values of mini-JINP: SID of CP (%) = (1.02 × SID of CP in mini-JINP) − 5.20 with r2 = 0.97 and p < 0.05; SID of Lys (%) = (1.12 × SID of Lys in mini-JINP) − 9.10 with r2 = 0.98 and p < 0.05; and SID of Met (%) = (1.08 × SID of Met in mini-JINP) − 4.27 with r2 = 0.96 and p < 0.05. The digestibility for most AA in feedstuffs for commercial pigs can be estimated using data from mini-JINP.

The objective of this review was to provide an overview of the effects of dietary fiber (DF) on reproductive performance in gestating sows. Dietary fibers have been suggested to modulate microbiota in the intestine and the immune system of gestating sows and to improve gut health. Thus, DF may help alleviate the adverse effects of the stressful production cycle of gestating sows. These benefits may subsequently result in improved reproductive performance of sows. Previous studies have reported changes in microbiota by providing gestating sows with DF, and the responses of microbiota varied depending on the source of DF. The responses by providing DF to gestating sows were inconsistent for antioxidative capacity, hormonal response, and inflammatory response among the studies. The effects of DF on reproductive performance were also inconsistent among the previous studies. Potential reasons contributing to these inconsistent results would include variability in reproductive performance data, insufficient replication, influence of other nutrients contained in the DF diets, characteristics of DF, and experimental periods. The present meta-analysis suggests that increasing the total DF concentration by 10 percentage units (e.g., 12% to 22% as-fed basis) in gestating sow diets compared to the control group improves the litter born alive by 0.49 pigs per litter. However, based on the present review, questions remain regarding the benefits of fibers in gestating sow diets. Further research is warranted to clarify the mode of action of fibers and the association with subsequent reproductive performance in gestating sows.

The objectives of the present study were to determine the nutrient digestibility of fish meal, defatted black soldier fly larvae (BSFL), and adult flies and to develop equations for estimating in vitro nutrient digestibility of BSFL for pigs. in vitro digestion procedures were employed to mimic the digestion and absorption of nutrients in the pig intestine. Correlation coefficients between chemical composition and in vitro nutrient digestibility of BSFL were calculated. In Exp. 1, in vitro ileal digestibility (IVID) of dry matter (DM) and crude protein (CP) and in vitro total tract digestibility (IVTTD) of DM and organic matter in defatted BSFL meal were less (p < 0.05) than those in fish meal but were greater (p < 0.05) than those in adult flies. In Exp. 2, CP concentrations in BSFL were negatively correlated with ether extract (r = −0.91) concentration but positively correlated with acid detergent fiber (ADF; r = 0.98) and chitin (r = 0.95) concentrations. ADF and chitin concentrations in BSFL were negatively correlated with IVID of DM (r = −0.98 and −0.88) and IVTTD of DM (r = −1.00 and −0.94) and organic matter (r = −0.99 and −0.98). Prediction equations for in vitro nutrient digestibility of BSFL were developed: IVID of CP (%) = −0.95 × ADF (% DM) + 95 (r2 = 0.75 and p = 0.058) and IVTTD of DM (%) = −2.09 × ADF + 113 (r2 = 0.99 and p < 0.001). The present in vitro experiments suggest that defatted BSFL meal was less digestible than fish meal but was more digestible than adult flies, and nutrient digestibility of BSFL can be predicted using ADF as an independent variable.

Objective The objectives were to demonstrate that the nitrogen and energy in pig urine supplemented with hydrochloric acid (HCl) are not volatilized and to determine the minimum amount of HCl required for nitrogen preservation from pig urine. Methods In Exp. 1, urine samples of 3.0 L each with 5 different nitrogen concentrations were divided into 2 groups: 1.5 L of urine added with i) 100 mL of distilled water or ii) 100 mL of 6 N HCl. The urine in open plastic containers was placed on a laboratory table at room temperature for 10 d. The weight, nitrogen concentration, and gross energy concentration of the urine samples were determined every 2 d. In Exp. 2, three urine samples with different nitrogen concentrations were added with different amounts of 6 N HCl to obtain varying pH values. All urine samples were placed on a laboratory table for 5 d followed by nitrogen analysis. Results Nitrogen amounts in urine supplemented with distilled water decreased linearly with time, whereas those supplemented with 6 N HCl remained constant. Based on the linear broken-line analysis, nitrogen was not volatilized at a pH below 5.12 (standard error = 0.71 and p

The objectives were to determine the effects of dietary deoxynivalenol (DON) on apparent ileal digestibility (AID) of nutrients and to evaluate the efficacy of a bentonite (BEN) and a preservative blend (PB) product for alleviating DON effects on the nutrient digestibility of pigs. Twelve crossbred barrows with an initial body weight of 69.4 kg (standard deviation = 3.5) equipped with a T-cannula in the distal ileum were allotted a triplicated 4 × 2 incomplete Latin square design with four dietary treatments and two periods. Dietary treatments were (1) an uncontaminated diet, (2) a contaminated diet (CD) mainly based on contaminated wheat with 1.6 mg/kg DON, (3) CD + 0.25% PB consisting of preservation components as major sources, antioxidants, microorganisms, and amino acids (AA), and (4) CD + 0.25% BEN. The AID and ATTD of dry matter, organic matter, crude protein, most minerals, and most AA were not affected by DON contamination. Dietary DON decreased the AID and ATTD of sodium (p < 0.05) but were restored by supplementing the PB product (p < 0.05). The AID of zinc was increased (p < 0.05) by dietary DON, but supplementing BEN decreased zinc digestibility (p < 0.05). The AID of Arg, Ile, Thr, and Asp was decreased (p < 0.05) by BEN addition. In conclusion, dietary DON affected the digestibility of some minerals but not AA in pigs. Supplemental BEN can negatively affect the nutrient digestibility of some minerals and AA in pigs. The addition of a PB product in pig diets can restore digestibility of sodium but not of other nutrients. Based on these observations, feed additives for alleviating DON effects on nutrient digestibility of pigs can be carefully selected by swine diet formulators.

The objective was to evaluate the effects of benzoic acid, bromelain, adipic acid, and humic substance supplementation on nitrogen balance, urinary pH, slurry pH, and manure odorous compounds in pigs. Fifteen castrated male pigs with an initial body weight of 37.9 kg (standard deviation = 4.1) were individually housed in metabolism crates. The animals were allocated to a triplicated 5 × 2 incomplete Latin square design with 15 animals, 5 experimental diets, and 2 periods. The basal diet mainly consisted of corn, soybean meal, and rapeseed meal. Four experimental diets were prepared by supplementing each additive at a concentration of 10 g/kg at the expense of corn starch to the basal diet. Each period consisted of a 4-day adaptation period, a 24 h collection period for slurry sampling, and a 4-day collection period for feces and urine. The feces and urine collected for 24 h on day 5 were mixed at a ratio of fecal weight and urine weight to obtain slurry samples. The apparent total tract digestibility N in pigs fed the humic substance diet was the least (p < 0.05) compared to the other groups. The daily retained N and N retention as % ingested tended (p < 0.10) to be the lowest in the adipic acid group among the treatments. The urinary pH in pigs fed the adipic acid diet was less (p < 0.05) than that in other groups except the benzoic acid group. The slurry pH tended to differ among the treatment groups (p = 0.074) with the lowest value in the pigs fed the adipic acid diet. The concentrations of indole in slurry (p = 0.084) and isovalerate in feces (p = 0.062) tended to differ among the groups with the lowest values in the pigs fed the humic substance diet. In conclusion, adipic acid supplementation in pig diets can decrease urinary pH and slurry pH. Although benzoic acid and adipic acid have limited effects in reducing odorous compounds, humic substances have the potential to reduce some odorous compounds.

Objective The objective was to test additivity of digestible energy and nutrient concentrations in the hatchery byproduct mixture fed to nursery pigs. Methods In the previous studies, energy, phosphorus, calcium, and amino acid digestibility of infertile eggs, unhatched eggs, culled chicks, and a mixture of 3 hatchery byproduct ingredients was determined in nursery pigs (initial body weight = 9.4 to 14.2 kg). An additivity test was conducted using these determined values. Results No difference was observed between determined and predicted metabolizable energy values in the mixture (3,998 and 3,990 kcal/kg as-is basis, respectively). Measured standardized total tract digestible phosphorus in the mixture was less than the predicted value (4.5 vs 5.3 g/kg as-is basis, respectively; p

The objective of this study was to investigate the effects of supplemental mycotoxin-sequestering agents on growth performance and nutrient utilization in growing pigs fed deoxynivalenol (DON)-contaminated diets. Twelve barrows with an initial body weight of 35.5 kg (standard deviation = 1.3) were assigned to six dietary treatments in a replicated 6 × 5 incomplete Latin square design. Five experimental diets consisted of an uncontaminated diet (PC), a DON-contaminated diet at 6.89 mg/kg (NC), NC + bentonite 0.5%, NC + yeast cell wall 0.5%, and NC + a mixture product 0.5% which consisted of enzymes, microorganisms, minerals, and plant extracts. Pigs had ad libitum access to the five diets. In the last group, the PC diet was restrictedly provided to pigs at the quantity of feed consumption of the NC group. Average daily gain, average daily feed intake, and gain:feed were not affected by supplemental mycotoxin-sequestering agents except for the mixed product that tended to improve (p = 0.064) gain:feed in pigs fed DON-contaminated diets. The apparent total tract digestibility (ATTD) of dry matter was not affected by DON contamination or by supplemental mycotoxin-sequestering agents, whereas the ATTD of Ca was decreased (p = 0.032) by supplemental yeast cell wall in pigs fed DON-contaminated diets. The ATTD of P was greater (p = 0.042) in pigs fed the NC diet compared with the pigs fed the restricted amount of the PC diet. In conclusion, bentonite and yeast cell wall did not affect growth performance of pigs fed DON-contaminated diets, but a supplemental mixed product consisting of enzymes, microorganisms, minerals, and plant extracts partially alleviated the negative effects of dietary DON on the gain:feed of pigs. Calcium digestibility was decreased by supplemental yeast cell wall in pigs fed DON-contaminated diets. Based on the present work, the use of a mixed product consisting of enzymes, microorganisms, minerals, and plant extracts is suggested, and the reduction of Ca digestibility by yeast cell wall needs to be considered in diet formulations.

The objective of this study was to identify Hanwoo cattle via a deep-learning model using muzzle images. A total of 9230 images from 336 Hanwoo were used. Images of the same individuals were taken at four different times to avoid overfitted models. Muzzle images were cropped by the YOLO v8-based model trained with 150 images with manual annotation. Data blocks were composed of image and national livestock traceability numbers and were randomly selected and stored as train, validation test data. Transfer learning was performed with the tiny, small and medium versions of Efficientnet v2 models with SGD, RMSProp, Adam and Lion optimizers. The small version using Lion showed the best validation accuracy of 0.981 in 36 epochs within 12 transfer-learned models. The top five models achieved the best validation accuracy and were evaluated with the training data for practical usage. The small version using Adam showed the best test accuracy of 0.970, but the small version using RMSProp showed the lowest repeated error. Results with high accuracy prediction in this study demonstrated the potential of muzzle patterns as an identification key for individual cattle.

Objective The objectives of the present study were to determine digestible energy (DE), metabolizable energy (ME), and standardized total tract digestibility (STTD) of phosphorus (P) in meat meal (MM) and to compare these values with those in fish meal (FM), and soybean meal (SBM) fed to pigs. Methods Two experiments were conducted to determine energy concentrations and STTD of P in MM, FM, and SBM fed to growing pigs. In Exp. 1, DE and ME in the 3 test ingredients were measured using 24 barrows with initial body weight (BW) of 77.7±8.3 kg. A corn-based diet and 3 diets containing corn and 22% to 30% of each test ingredient were prepared. In Exp. 2, the STTD of P in the 3 test ingredients was measured using 24 barrows (90.9±6.6 kg BW). Three diets were formulated to contain each test ingredient as the sole source of P. Results In Exp. 1, the DE and ME values in MM (3,310 and 2,856 kcal/kg dry matter [DM]) were less (p

The present work aimed to assess the nutrient digestibility of soybean meal (SBM) products based on in vitro procedures. Two-step and three-step in vitro assays were performed to mimic the digestion and absorption of nutrients in the digestive tracts of growing swine. The two-step in vitro method was modified to reflect the digesta retention time and digestive enzymes of nursery piglets by decreasing incubation periods and digestive enzymes to half of those in the procedure for growing pigs and was used to determine the crude protein (CP) digestibility of nursery piglets. The seven ingredients included conventional SBM, thermo-mechanically processed SBM (TSBM), and five sources of fermented SBM (FSBM). The five sources of FSBM were produced using different microorganisms for fermentation, namely: (1) Pediococcus pentosaceus and Bacillus subtilis, (2) Enterococcus faecium (FSBM-EF), (3) Aspergillus oryzae and Bacillus subtilis, (4) Aspergillus oryzae, and (5) Bacillus licheniformis. Based on the conventional procedure, the in vitro ileal disappearance of CP in TSBM was greater (p < 0.05) compared with that in FSBM sources. Based on the in vitro assays for total tract digestibility, organic matter in TSBM was better digested (p < 0.05) compared with that in FSBM except for FSBM-EF. Based on the in vitro procedure for nursery piglets, the ileal disappearance of CP in TSBM was greater (p < 0.05) than that in the other SBM products. Taken together, thermo-mechanical processing rather than microbial fermentation of SBM improves the nutrient digestibility of SBM, particularly in nursery pigs.

The aims of this study were to determine in vitro ileal disappearance (IVID) of dry matter (DM) and crude protein (CP) in commercial dog diets and to develop equations for predicting the IVID of DM and CP in dog diets based on chemical composition. Eighteen commercial dog diets were analyzed for IVID of DM and CP using a two-step in vitro procedure for dogs. The diet samples in flasks with digestive enzymes were incubated for 2 h and 4 h to simulate digestion in the stomach and the small intestine, respectively. The contents of CP, ether extract, neutral detergent fiber (NDF), and ash in the diets ranged from 14.4 to 42.5%, 3.5 to 23.5%, 6.4 to 34.6%, and 4.9 to 10.0%, respectively, on an as-is basis. The NDF contents were negatively correlated with the IVID of DM and CP (r = −0.73 and r = −0.62, respectively; p < 0.05). The most suitable prediction equations for the IVID of DM and CP in the dog diets were: IVID of DM (%) = 81.33 + 0.46 × CP − 0.77 × NDF, R2 = 0.78; IVID of CP (%) = 81.25 + 0.33 × CP − 0.49 × NDF, R2 = 0.64, where all nutrients were in % on an as-is basis. In conclusion, dry matter and protein utilization of dog diets based on in vitro digestibility assays can be estimated fairly well using protein and fiber concentrations as independent variables.

The objective of this study was to develop equations for estimating ileal digestible crude protein (CP) and metabolizable energy (ME) contents of meat meal (MM) and meat and bone meal (MBM) as feed ingredients for pigs based on in vitro assays. Test ingredients were 4 sources of MM and 3 sources of MBM. Ash and CP contents of the ingredients ranged from 3.8% to 33.1% and 46.8% to 82.9% (as-is basis), respectively. In vitro ileal disappearance (IVID) of CP was determined and ileal digestible CP content was calculated by multiplying CP content by IVID of CP. In vitro total tract disappearance (IVTTD) of dry matter (DM) was determined and ME was calculated using gross energy, CP contents, and IVTTD of DM. The IVID of CP and IVTTD of DM ranged from 77.2% to 88.7% and from 82.7% to 92.4%, respectively. Calculated ileal digestible CP and ME contents ranged from 37.8% to 73.5% DM and 2,405 to 3,905 kcal/kg DM, respectively. Ash contents were negatively correlated (P

Background: Alternative feed ingredients are widely used in swine diets to lower feed costs, but these ingredients contain a large quantity of non-starch polysaccharides. Supplemental xylanase is known to break down non-starch polysaccharides. However, the effects of exogenous xylanase from Bacillus subtilis on various feed ingredients have rarely been compared. Objective: To evaluate the effects of supplemental xylanase on in vitro disappearance of dry matter (DM) in various feed ingredients for pigs. Methods: Nine feed ingredients were used to measure in vitro ileal disappearance and in vitro total tract disappearance of DM. Each ground ingredient was supplemented with either supplemental xylanase (9,000 U/g) or cornstarch at 1.0%. Results: Supplemental xylanase increased in vitro ileal disappearance of DM in wheat, barley, wheat flour, and wheat bran (p

Objective The objectives were to develop prediction equations for digestible energy (DE) and metabolizable energy (ME) of feed ingredients and diets for pigs based on chemical composition and to evaluate the accuracy of the equations using in vivo data. Methods A total of 734 data points from 81 experiments were employed to develop prediction equations for DE and ME in feed ingredients and diets. The CORR procedure of SAS was used to determine correlation coefficients between chemical components and energy concentrations and the REG procedure was used to generate prediction equations. Developed equations were tested for the accuracy according to the regression analysis using in vivo data. Results The DE and ME in feed ingredients and diets were most negatively correlated with acid detergent fiber or neutral detergent fiber (NDF; r = −0.46 to r = −0.67; p

Objective The objective of this study was to investigate the influence of dietary lead (Pb) supplementation and feeding period on growth performance, organ weight, and Pb accumulation in pigs. Methods In a 56-day feeding experiment, a total of 48 barrows with initial body weight 10.4±0.6 kg were allotted to 2 dietary treatments (0 and 200 mg/kg of supplemental Pb) in a completely randomized design with 6 replicates. Body weight and feed intake were recorded to calculate growth performance. At the end of each 14 day-period (on days 14, 28, 42, and 56), an animal was randomly selected from each pen and slaughtered to collect blood samples, hair samples, left 5th rib, heart, liver, kidneys, lungs, and longissimus dorsi muscle samples. Results Average daily gain and average daily feed intake were reduced (p

Objective The objective was to determine standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in cereal grains and various co-products fed to growing pigs. Methods Ten feed ingredients tested were barley (9.3% CP), lupin kernels (31.1% CP), and wheat (11.3% CP) as cereal grains, and 2 sources of corn gluten feed produced in China (21.6% CP) and Korea (24.6% CP), corn gluten meal (65.3% CP), lupin hulls (11.6% CP), rice bran (14.5% CP), soybean meal (44.8% CP), and wheat bran (15.4% CP) as co-products. Ten experimental diets were formulated to contain each ingredient as a sole source of N and an N-free diet was used to correct basal endogenous losses of CP and AA. All diets also contained 0.5% Cr2O3 as an indigestible index. A replicated 11×6 incomplete Latin square design with 11 dietary treatments, 6 periods, and 22 animals was employed. Twenty-two barrows with an initial body weight of 64.6±4.9 kg were equipped with a T-cannula in the distal ileum. An experimental period consisted of a 4-d adaptation period and a 2-d collection period. Results The SID of CP in the barley, lupin kernels, wheat, 2 sources of corn gluten feed, corn gluten meal, lupin hulls, rice bran, soybean meal, and wheat bran were 84.7%, 90.5%, 90.4%, 77.4%, 74.6%, 89.5%, 90.4%, 74.4%, 86.9%, and 63.4% (standard error of the mean [SEM] = 5.3, p = 0.006), respectively. The respective SID values of Lys were 75.5%, 88.4%, 83.9%, 74.7%, 62.4%, 80.3%, 83.9%, 78.5%, 88.0%, and 71.2% (SEM = 3.3, p

Objective The objectives were to investigate correlations between energy digestibility (digestible energy [DE]:gross energy [GE]) and various fiber types including crude fiber (CF), total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF), neutral detergent fiber (NDF), and acid detergent fiber (ADF), and to develop prediction equations for estimating DE in feed ingredients and diets for growing pigs. Methods A total of 289 data with DE values and chemical composition of feeds from 39 studies were used to develop prediction equations for DE. The equations were validated using values provided by the National Research Council. Results The DE values in feed ingredients ranged from 2,011 to 4,590 kcal/kg dry matter (DM) and those in diets ranged from 2,801 to 4,203 kcal/kg DM. In feed ingredients, DE:GE was negatively correlated (p

The objectives were to determine the effects of graded inclusion rates of wheat bran (WB) on apparent ileal (AID), apparent total tract (ATTD), and hindgut digestibility of nutrients and tested the influence of ileal digesta collection on proceeding fecal nutrient digestibility in pigs. Six barrows with an initial mean body weight of 70.7 ± 5.7 kg fitted with an ileal T-cannula were used. The animals were assigned to a replicated 3 × 3 Latin square design with three diets and three periods. A basal diet was based mainly on wheat, soybean meal, and cornstarch. Two additional diets were formulated to contain 20 or 40% of WB at the expense of cornstarch. Each experimental period consisted of a seven-day adaptation period and a four-day collection period. After the adaptation period, fecal samples were collected on day 8, and ileal digesta were collected on days 9 and 10. Another set of fecal samples was collected on day 11 to determine the influence of ileal digesta collection on proceeding total tract nutrient digestibility. The AID of energy, dry matter (DM), organic matter (OM), crude protein, and phosphorus linearly decreased (p < 0.05) with an increasing inclusion rate of WB from 0 to 40%. The ATTD of energy, DM, OM, crude protein, ether extract, and phosphorus linearly decreased (p < 0.01) as the inclusion rate of WB increased. Hindgut digestibility of DM, OM, and ether extract linearly increased (p < 0.05) with an increasing inclusion rate of WB. The ATTD of GE and most nutrients did not differ between the two fecal collection periods of before and after ileal digesta collection. Taken together, the inclusion of a fiber-rich ingredient reduced ileal and fecal digestibility of nutrients but increased hindgut digestibility of some nutrients, and total tract digestibility of nutrients did not differ whether the fecal samples were collected before or after two days of ileal digesta collection in pigs.

The objective was to determine the effects of drying and blanching methods on the nutrient utilization of black soldier fly larva (BSFL; Hermetia illucens) meal by pigs using in vitro assays. Two-step and three-step in vitro assays were employed to simulate the gastrointestinal tract of pigs. Four BSFL meals were prepared using the following pretreatment methods: (1) microwave drying at 80 °C for 32 min, (2) hot-air drying at 60 °C for 17 h, (3) blanching for 5 min in boiling water and hot-air drying at 60 °C for 17 h, and (4) 2% citric acid solution blanching for 5 min in boiling solution and hot-air drying at 60 °C for 17 h. After the drying process, each BSFL was defatted and ground to obtain BSFL meals. The nitrogen (N) concentration in the test ingredients ranged from 8.5 to 9.4%, and the ether extract ranged from 6.9 to 11.5% on an as-is basis. The amino acid (AA) concentration in the BSFL meals ranged from 2.80 to 3.24% for Lys and 0.71 to 0.89% for Met on an as-is basis. Hot-air-dried BSFL meal had a greater in vitro ileal disappearance (IVID) of N compared with microwave-dried BSFL meal (p < 0.05). However, blanched BSFL meals in water or 2% citric acid solution before hot-air drying had a lower (p < 0.05) IVID of N compared with microwave-dried or hot-air-dried BSFL meal. Blanched BSFL meals in water or 2% citric acid solution before hot-air drying showed a lower (p < 0.05) in vitro total tract disappearance of dry matter and organic matter compared with microwave-dried or hot-air-dried BSFL meal. Microwave-dried BSFL meal had a lower (p < 0.05) IVID of indispensable AA, except for His, Lys, Met, and Phe, compared with hot-air-dried BSFL meals. However, blanched BSFL meals in water or 2% citric acid solution before hot-air drying showed a lower (p < 0.05) IVID of indispensable AA compared with microwave-dried or hot-air-dried BSFL meal. In conclusion, hot-air-dried BSFL meal presented greater nutrient utilization compared with microwave-dried BSFL meal for pigs. However, blanching in water or citric acid solution negatively affected the nutrient digestibility of BSFL meal based on in vitro assays.

The present study was conducted to test whether the dietary supplementation of β-mannanase affects amino acids (AA) digestibility, metabolizable energy (ME) contents of corn, wheat, soybean meal, distillers dried grains with solubles, and palm kernel meal (PKM), nutrient digestibility, and growth performance of pigs. In Exp. 1, 22 cannulated pigs were used for 10 dietary treatments including 5 feed ingredients and 2 β-mannanase concentrations (0 and 0.5 g/kg of the diet) in 6 periods in an incomplete Latin square design to determine the AA and energy digestibility. In Exp. 2, 200 growing pigs were randomly allotted to 4 treatments with 2 nutrient levels (high and low) and 2 concentrations of β-mannanase (2 × 2 factorial arrangement) in 2 phases (phase 1, d 0 to 21; and phase 2, d 22 to 42). In Exp. 1, β-mannanase increased the mean standardized ileal digestibility (SID) of AA in all feed ingredients. The amount of digestible energy was increased (P

Objective The objective was to determine standardized ileal digestibility (SID) of amino acids (AA) in 11 plant protein sources fed to growing pigs. Methods Eleven feed ingredients used were sesame meal, two sources of soybean meal (SBM) produced in the Republic of Korea, a source of SBM produced in India, high-protein distillers dried grains (HPDDG), perilla meal, canola meal, copra meal, corn germ meal, palm kernel expeller, and tapioca distillers dried grains (TDDG). Experimental diets were prepared to contain each test ingredient as a sole source of AA, and a nitrogen-free diet was also prepared to estimate the basal ileal endogenous losses of AA. Twelve barrows surgically fitted with T-cannulas at the distal ileum with an initial body weight of 29.0 kg (standard deviation = 3.0) were individually housed in metabolism crates equipped with a feeder and a nipple drinker. A 12×9 incomplete Latin square design was employed with 12 experimental diets, 12 animals, and 9 periods. After a 5-d adaptation period, ileal digesta were collected on d 6 and 7 in each experimental period. Results Values for apparent ileal digestibility of most indispensable AA in three sources of SBM were greater compared with other test ingredients except HPDDG and canola meal (p

ABSTRACT The aim of the present study was to determine the effects of supplemental protease on in vitro disappearance of dry matter (DM) and crude protein (CP) in feed ingredients for pigs. The test ingredients were three sources of feather meal (FM 1, FM 2, and FM 3), meat and bone meal, soybean meal, corn dried distillers grains with solubles, copra meal (CM), palm kernel meal, wheat, and corn. In experiment 1, in vitro ileal disappearance (IVID) of DM and CP were determined to represent the digestion and absorption of the stomach and small intestine of pigs. In experiment 2, in vitro total tract disappearance (IVTTD) of DM was determined to represent the digestion and absorption of the stomach, small intestine, and large intestine of pigs. The control group had 99% of a test ingredient and 1% of wheat bran. Protease originating from Bacillus spp. was supplemented to the control diet at the expense of wheat bran to make a treatment group (100,000 U/kg of sample). Protease supplementation increased the IVID of DM in FM 1 (39.8 vs. 37.5%) and CM (45.0 vs. 42.2%). In the protease supplementation group, the IVID of CP also increased in FM 1 (48.0 vs. 43.8%) and CM (85.3 vs. 77.5%). The supplemental protease increased the IVTTD of DM in FM 1 (43.9 vs. 42.1%), FM 3 (55.6 vs. 52.7%), and meat and bone meal (85.9 vs. 84.3%). Therefore, nutrient utilization of FM and CM can be improved by supplemental protease originating from Bacillus spp. based on in vitro assays.

The objective of this study was to determine the efficacy of mycotoxin sequestering agents for aflatoxin B1 (AFB1), deoxynivalenol (DON), and zearalenone (ZEA) using an in vitro method. The twelve toxin sequestering agents tested were seven bentonite products (bentonite A, B, C, D, E, F, and G), two aluminosilicate products (aluminosilicate A and B), a heulandite product, an activated charcoal product, and a yeast cell wall product. A two-step in vitro procedure was employed to mimic the conditions of temperature, pH, and digestive enzymes in the stomach and small intestine of pigs. All mycotoxin sequestering agents tested were able to bind to AFB1 with a high efficacy (>92%). The DON sequestering rate of activated charcoal (99.1%) was greater (p < 0.05) than that of other products. The ZEA sequestering rate of bentonite F (97.0%), aluminosilicate A (99.6%), and activated charcoal (100.0%) was the greatest (p < 0.05) among the tested mycotoxin sequestering agents. Overall, most mycotoxin sequestering agents had the ability to bind to AFB1, but most products, except activated charcoal, failed to sequester DON and ZEA.

Objective An experiment was conducted to determine digestible energy (DE) and metabolizable energy (ME) of different byproduct feed ingredients fed to growing pigs, and to generate prediction equations for the DE and ME in feed ingredients. Methods Twelve barrows with an initial mean body weight of 31.8 kg were individually housed in metabolism crates that were equipped with a feeder and a nipple drinker. A 12×10 incomplete Latin square design was employed with 12 dietary treatments, 10 periods, and 12 animals. A basal diet was prepared to mainly contain the corn and soybean meal (SBM). Eleven additional diets were formulated to contain 30% of each test ingredient. All diets contained the same proportion of corn:SBM ratio at 4.14:1. The difference procedure was used to calculate the DE and ME in experimental ingredients. The in vitro dry matter disappearance for each test ingredient was determined. Results The DE and ME values in the SBM sources were greater (p

The objectives of the present work were to assess the accuracy of previously published equations for predicting effects of deoxynivalenol (DON) on the growth performance changes of pigs and to update equations based on recently published data. A total of 59 data were employed for the validation of previously published equations. These data were used to update the equations. The REG and CORR procedures of SAS were used. In the present validation test, a linear bias was significant (p < 0.05), indicating that prediction errors were not consistent across the data ranges. The intercept for ΔFI (−7.75 ± 1.19, p < 0.01) representing a mean bias was less than 0, indicating that the predicted mean of ΔFI was greater than the measured mean of ΔFI. Dietary DON concentrations had negative correlations with ΔWG (r = −0.79; p < 0.01) and ΔFI (r = −0.71; p < 0.01). Updated prediction equations were: ΔWG = −5.93 × DON with r2 = 0.77 and ΔFI = −4.42 × DON with r2 = 0.68. In conclusion, the novel equations developed in this study might accurately predict effects of dietary DON on the performance changes of pigs.

The objectives of the present study were to determine the influence of thermal and non-thermal processing procedures on in vitro ileal disappearance (IVID) of dry matter (DM) and crude protein (CP) in chicken meat as dog foods using 2-step in vitro assays. In thermal processing experiments, IVID of DM and CP in chicken meat thermally processed at 70, 90, and 121 °C, respectively, with increasing processing time was determined. For non-thermal processing experiments, IVID of DM and CP in chicken meat processed by high-pressure, ultraviolet-light emitting diode (UV-LED), electron-beam, and gamma-ray was determined. Thermal processing of chicken meat at 70, 90, and 121 °C resulted in decreased IVID of CP (p < 0.05) as heating time increased. In non-thermal processing experiment, IVID of CP in chicken meat was not affected by high-pressure processing or UV-LED radiation. In vitro ileal disappearance of CP in electron-beam- or gamma-ray-irradiated chicken meat was not affected by the irradiation intensity. Taken together, ileal protein digestibility of chicken meat for dogs is decreased by thermal processing, but is minimally affected by non-thermal processing methods.

Amino acid (AA)-to-crude protein (CP) ratios in soybean meal (SBM) may be different for different sources of SBM depending on the presence of additional hulls. Therefore, this study was conducted to develop novel two-slope equations to predict the concentrations of AAs in SBM using CP as an independent variable. Regression analyses were performed with each AA in SBM as the dependent variable and the CP as the independent variable. Among all AAs, the predicted Lys in SBM (% dry matter (DM)) was: Lys = 3.19 − 0.026 × (51.88 − CP) where CP < 51.88% DM and Lys = 3.19 + 0.072 × (CP − 51.88) where CP > 51.88% DM with R2 = 0.51 and p < 0.001. In conclusion, the novel equations provided reasonable estimates of the AA concentrations from different ranges of CP in SBM.

The objectives were to determine effects of a hatchery byproduct mixture (HBM) on growth performance and to measure digestible energy concentrations in various HBM. In the growth performance experiment, 96 pigs (initial body weight = 9.6 ± 0.8 kg) were assigned to 4 dietary treatments in a randomized complete block design with 6 blocks. Each treatment consisted of 6 replicate pens with 4 pigs comprising 2 barrows and 2 gilts. Pigs were fed graded concentrations of HBM at 0%, 3.33%, 6.67%, and 10.00% for 14 days. In the energy digestibility experiment, 10 barrows (initial body weight = 11.5 ± 0.4 kg) were employed to determine digestible energy in HBM. A basal diet based on corn and soybean meal and 4 additional diets containing 25% of 4 different HBM were prepared. The marker-to-marker method was employed for total collection and the experimental design was a replicated 5 × 4 Latins square design. Growth performance was not compromised as the inclusion rate of HBM increased up to 10%. Digestible energy of HBM ranged from 2772 to 3887 kcal/kg as-is basis. In conclusion, HBM can be used in nursery pig diets and different energy values should be used for each HBM.

Background: phytase supplementation to sow diets may improve digestibility of P and Ca and bone integrity of suckling piglets. Objective: two experiments were conducted to investigate the effect of phytase supplementation to gestation and lactation diets on reproductive performance, apparent total tract digestibility (ATTD) of P, Ca, and N in sows and bone characteristics in sows and piglets. Methods: in Experiment 1, a multistate study involving 204 sows was conducted at 3 cooperating research stations. Sows were assigned to 1 of 4 treatments at breeding: 1) negative control [low Ca and available P (aP) diet], 2) Treatment 1 plus 300 FTU of supplemental phytase/kg of diet, 3) Treatment 1 plus 600 FTU of supplemental phytase/kg of diet, and 4) positive control (normal Ca and aP diet). In Experiment 2, a total of 16 sows (Yorkshire × Landrace; average parity, 2.3) were randomly allotted to 1 of 3 treatments based on body weight (BW) and parity at d 34 to 52 of gestation. Treatments were: 1) low Ca and aP diet, 2) low Ca and aP diet with 500 FTU of supplemental phytase/kg of diet, and 3) normal Ca and aP diet. Results: in Experiment 1, the number of total born (p0.10). In gestation the ATTD of P was lower for the low Ca and aP without phytase group than the normal Ca and aP (p

The objective was to measure energy concentrations and standardized total tract digestibility (STTD) of phosphorus (P) in hatchery byproducts. In Experiment 1, 20 nursery barrows were used to measure energy concentrations in hatchery byproducts. A basal diet based on corn and dried whey and four additional diets containing 25% of infertile eggs, unhatched eggs, culled chicks, or a mixture of the three hatchery byproducts were prepared. In Experiment 2, the STTD of P was measured using 20 nursery barrows. Four diets containing 25% of the same hatchery byproducts used in Experiment 1 as the sole source of P were prepared, and a P-free diet was prepared to measure basal endogenous losses of P. The marker-to-marker method was employed for total collection. Metabolizable energy in culled chicks was the greatest (4560 kcal/kg as-is basis; p < 0.05), whereas infertile eggs had the lowest value (2645 kcal/kg as-is basis; p < 0.05). The STTD of P in infertile eggs (81.7%) was greater than that in unhatched eggs, culled chicks, and the mixture (61.6, 53.9, and 47.4%, respectively; p < 0.05). In conclusion, culled chicks had the greatest metabolizable energy and infertile eggs had the greatest phosphorus digestibility among the test ingredients.

Dietary mycotoxins have been shown to cause detrimental effects in swine health and production. The objective of this study was to develop tools for predicting the effects of aflatoxin (AFL), deoxynivalenol (DON) and zearalenone (ZON) on feed intake (FI) and weight gain (WG) changes using a meta-analysis approach. A total of 80 and 63 observations were extracted from 18 experiments testing the effects of AFL on FI and WG, respectively, and the differences of AFL concentrations between the control and treatment groups ranged from 0.02 to 2.5 mg/kg. A total of 117 and 113 observations from 20 experiments were used for testing the effects of DON on FI and WG, respectively. The differences of DON concentrations between the control and treatment groups ranged from 0.5 to 10.5 mg/kg. A total of 16 and 17 observations from 18 experiments were used for testing the effects of ZON on FI and WG, respectively, and the differences of ZON concentrations between the control and treatment groups ranged from 0.2 to 9.0 mg/kg. Effects of experiment, initial body weight, and experimental period were not significant for developing prediction equations for the changes of FI and WG. The models developed for predicting FI and WG changes (ΔFI and ΔWG) as % by AFL concentrations as mg/kg were: ΔFI = -24.9 × AFL - 1.7 with r² = 0.70 and p

Summary Background: accurate estimations of basal endogenous losses (BEL) of amino acids (AA) are important for the calculation of standardized ileal digestibility values. Objectives: to address the influence of body weight (BW) and feed intake on BEL of crude protein (CP) and AA and to develop prediction equations for BEL of CP and AA in pigs fed nitrogen-free diets. Methods: based on data derived from 34 research papers, prediction equations for BEL of CP and AA were generated using BW and feed intake per maintenance feed intake (FI:MFI) based on energy concentration as independent variables. Results: initial BW (IBW) and FI:MFI ranged from 13.8 to 109.8 kg and from 1 to 5, respectively. Mean values for BEL of CP, Lys, Met, Thr, and Trp were 17.2 (CV = 50.9%), 0.42 (CV = 56.0%), 0.14 (CV = 80.8%), 0.55 (CV = 41.3%), and 0.14 (CV = 57.5%) g/kg dry matter intake (DMI), respectively. The FI:MFI was negatively correlated with BEL of CP and AA except Met (r < -0.39; p < 0.05). Prediction equations for estimating the BEL of CP and AA (g/kg DMI) developed were: BEL of CP = 47.5 - (8.09 × 10-2 × IBW) - (8.83 × FI:MFI) with R2 = 0.32 and p = 0.011; BEL of Lys = 0.979 - (1.00 × 10-3 × IBW) - (0.174 × FI:MFI) with R2 = 0.24 and p = 0.014; BEL of Thr = 1.09 - (1.82 × 10-3 × IBW) - (0.153 × FI:MFI) with R2 = 0.28 and p = 0.007; and BEL of Trp = 0.552 - (1.11 × 10-3 × IBW) - (0.120 × FI:MFI) with R2 = 0.47 and p = 0.002. Conclusion: based on the equations above, IBW and FI:MFI clearly decrease BEL of CP and AA. The equations provided in this paper may be used for estimating BEL of CP and AA. Key words: modeling, standardized ileal digestibility, swine. Resumen Antecedentes: la estimación precisa de las pérdidas endógenas basales (BEL) de aminoácidos (AA) es importante para calcular los valores de la digestibilidad ileal estandarizada. Objetivos: establecer la influencia del peso corporal y el consumo de alimento sobre las BEL de proteína cruda (CP) y AA, y desarrollar ecuaciones de predicción para las BEL de CP y AA en cerdos alimentados con dietas libres de nitrógeno. Métodos: basados en información proveniente de 34 artículos científicos, fueron generadas ecuaciones de predicción para las BEL de CP y AA usando el peso corporal (BW) y el consumo de alimento por consumo de mantenimiento (FI:MFI) con base en la concentración de energía como variables independientes. Resultados: BW y FI:MFI variaron de 13,8 a 109,8 kg y de 1 a 5, respectivamente. Los valores de la media para BEL de CP, Lys, Met, Thr, y Trp fueron 17,2 (CV = 50,9%), 0,42 (CV = 56,0%), 0,14 (CV = 80,8%), 0,55 (CV = 41,3%), y 0,14 (CV = 57,5%) g/kg de consumo de materia seca (DMI), respectivamente. El FI:MFI se correlacionó negativamente con las BEL de CP y AA excepto con la Met (r < -0,39; p < 0,05). Las ecuaciones de predicción para estimar las BEL de la CP y AA (g/kg DMI) desarrolladas fueron: BEL de CP = 47,5 - (8,09 × 10-2 × IBW) - (8,83 × FI:MFI) con un R2 = 0,32 y un p = 0,011; BEL de Lys = 0,979 - (1,00 × 10-3 × IBW) - (0,174 × FI:MFI) con un R2 = 0,24 y un p = 0,014; BEL de Thr = 1,09 - (1,82 × 10-3 × IBW) - (0,153 × FI:MFI) con un R2 = 0,28 y un p = 0,007; y las BEL de Trp = 0,552 - (1,11 × 10-3 × IBW) - (0,120 × FI:MFI) con un R2 = 0,47 y un p = 0,002. Conclusión: con base en las anteriores ecuaciones, el IBW y el FI:MFI claramente redujeron las BEL de CP y AA. Las ecuaciones suministradas en este artículo pueden ser utilizadas para estimar las BEL de CP y AA. Palabras clave: digestibilidad ileal estandarizada, modelación, porcinos. Resumo Antecedentes: a estimação precisa das perdas endógenas basais (BEL) de aminoácidos (AA) é importante para calcular os valores da digestibilidade ileal padronizada. Objetivos: estabelecer a influencia do peso corporal e do consumo de alimento sobre as BEL da proteína bruta (CP) e AA, e desenvolver equações de predição para as BEL da CP e AA em porcos alimentados com dietas livres de nitrogênio. Métodos: baseados em informação proveniente de 34 artigos científicos, foram geradas equações de predição para as BEL da CP e AA usando o peso corporal (BW) e o consumo de alimento por consumo de manutenção (FI:MFI) com base na concentração de energia como variáveis independentes. Resultados: BW e FI:MFI variaram de 13,8 a 109,8 kg e de 1 a 5, respectivamente. Os valores da média para BEL da CP, Lys, Met, Thr, e Trp foram 17,2 (CV = 50,9%), 0,42 (CV = 56,0%), 0,14 (CV = 80,8%), 0,55 (CV = 41,3%), e 0,14 (CV = 57,5%) g/kg de consumo de matéria seca (DMI), respectivamente. O FI:MFI teve correlação negativa com as BEL da CP e AA exceto com a Met (r < -0,39; p < 0,05). As equações de predição para estimar as BEL da CP e AA (g/kg DMI) desenvolvidas foram: BEL da CP = 47,5 - (8,09 × 10-2 × IBW) - (8,83 × FI:MFI) com um R2 = 0,32 e um p = 0,011; BEL de Lys = 0,979 - (1,00 × 10-3 × IBW) - (0,174 × FI:MFI) com um R2 = 0,24 e um p = 0,014; BEL de Thr = 1,09 - (1,82 × 10-3 × IBW) - (0,153 × FI:MFI) com um R2 = 0,28 e um p = 0,007; e as BEL de Trp = 0,552 - (1,11 × 10-3 × IBW) - (0,120 × FI:MFI) com um R2 = 0,47 e um p = 0,002. Conclusão: Com base nas anteriores equações, o IBW e o FI:MFI claramente diminuíram as BEL da CP e AA. As equações fornecidas neste artigo podem ser utilizadas para estimar as BEL do CP e AA. Palavras chave: digestibilidade ileal padronizada, modelação, porcinos.

The objective of the present review is to provide an overview on the effects of the addition of acidifiers to nursery pig diets. Researchers have proposed that dietary acidifiers decrease pH in the stomach and the lower gastrointestinal tract (GIT) of weanling pigs, and thus, protect the host from pathogenic invasion and proliferation and improve nutrient digestion. These benefits may subsequently result in improved growth performance of weanling pigs. In experiments on nursery pigs, dietary acidifiers decreased stomach pH at least to a small extent, but had little influence on the pH of the lower GIT. Studies found no observable changes in microbial populations upon providing weanling pigs with dietary acidifiers, but they found a slight reduction in lactobacilli or lactic acid-producing bacteria throughout the GIT. Dietary acidifiers improved protein digestion in many cases despite variable results regarding the digestibility of amino acids and improved growth performance. Such positive responses were more apparent during the first or second week of experiments than the later stages. However, growth responses were inconsistent among different sources and varying inclusion rates of acidifiers. Information on the dietary acidifiers’ mode of action is quite limited, and large variations exist in results regarding the effects of dietary acidifiers. Based on the present review, the benefit of dietary acidifiers for nursery pigs is not entirely convincing. Further research is required to clarify the acidifiers’ mode of action and its association with subsequent growth performance in weanling pigs.El objetivo de esta revisión es ofrecer una visión general sobre los efectos de la adición de acidificantes orgánicos en la dieta de cerdos destetos. Se ha propuesto que los acidificantes dietarios reducen el pH en el estómago y tracto gastrointestinal (TGI) de los cerdos recién destetados, y por lo tanto protegen al huésped de la invasión y proliferación de patógenos, al tiempo que mejoran la digestión de nutrientes. Estos beneficios podrían resultar en una mejora del crecimiento de dichos animales. En experimentos con cerdos destetados, los acidificantes dietarios han mostrado que pueden disminuir el pH del estómago, al menos en pequeña medida, pero han tenido poca influencia en el pH del tracto gastrointestinal posterior. Algunos estudios no encontraron cambios observables en las poblaciones microbianas al ofrecer acidificantes en la dieta a estos animales, pero se reporta una ligera reducción de los lactobacilos o bacterias lácticas productoras de ácido en todo el tracto gastrointestinal. En muchos casos, los acidificantes parecen mejorar la digestión proteica, a pesar de ofrecer resultados variables con respecto a la digestibilidad de los aminoácidos y mejora del crecimiento. Tales respuestas positivas parecen ser más evidentes durante la primera o segunda semana de los experimentos que en etapas posteriores. Sin embargo, la respuesta en crecimiento ha sido inconsistente respecto a diferentes fuentes y niveles de inclusión de acidificantes. La información sobre el modo de acción de los acidificantes dietarios es bastante limitada y existen grandes variaciones en los resultados respecto a sus efectos. De acuerdo con esta revisión, el beneficio potencial de los acidificantes dietarios en cerdos destetados no está del todo justificado. Se requiere investigación adicional para aclarar su modo de acción y su relación con el crecimiento de los cerdos recién destetados.O objetivo desta revisão é fornecer uma visão geral dos efeitos da adição de acidificantes orgânicos na dieta de leitões desmamados. Tem sido proposto que a dieta acidificante reduz o pH do estômago e do trato gastrointestinal (TGI) de leitões desmamados, e assim proteger o hospedeiro da invasão e proliferação de patogênicos, melhorando a digestão dos nutrientes. Estes podem resultar em um maior crescimento destes animais. Em experimentos com suínos desmamados, a acidificação da dieta têm sido mostrados para diminuir o pH do estômago, pelo menos em pequena medida, mas tiveram pouca influência sobre o pH do trato gastrointestinal posterior. Alguns estudos não encontraram alterações observáveis em populações microbianas ao oferecer acidificantes na dieta, mas tem-se reportado uma ligeira redução de lactobacilos ou bactérias lácticas produtoras de ácido em tudo o trato gastrointestinal. Em muitos casos, os acidificantes parecem melhorar a digestão de proteínas, apesar de oferecer resultados variáveis com respeito à digestibilidade dos aminoácidos e melhora o crescimento. ais respostas positivas parecem ser mais evidentes durante a primeira ou segunda. No entanto, a resposta de crescimento tem sido inconsistente com relação a diferentes fontes e níveis de inclusão de acidificantes. A Informação sobre o modo de ação dos acidificantes na dieta é bastante limitado e existem grandes variações nos resultados sobre seus efeitos. De acordo com essa análise, o benefício potencial de alimentar leitões desmamados com acidificantes não é inteiramente justificado. Mais pesquisas são necessárias para esclarecer seu modo de ação e sua relação com o crescimento de leitões desmamados.

A novel chamber system was developed to measure gas emission from small ruminants and to measure nutrient digestibility simultaneously. Animal behavior can also be easily observed in this system through transparent panels. The system is composed of respiration-metabolism chambers, gas sampling and analysis units, and a data acquisition unit. A recovery test was performed using standard methane (CH4) gas. Values for recovery rate of CH4 gas ranged from 96.7 to 99.6% in 8 replications, and the mean value was 98.1% (coefficient of variation = 1.3%). A preliminary animal experiment was also conducted using 4 Korean native black goats with a mean body weight of 23.5 kg. The goats consumed a 50:50 mixture of forage and concentrate, and daily feed allowance was 2% body weight. Dry matter digestibility was 70.9%, and the mean CH4 gas production was 11.6 g/day that was calculated to be 24.7 g/kg dry matter intake. Using this system, researchers can accurately and efficiently conduct various experiments measuring methane emission, nutrient digestibility, or both in small ruminant animals including goats, sheep, and deer.Se ha desarrollado un sistema de cámaras para medir simultáneamente la emisión de gases de pequeños rumiantes y la digestibilidad de nutrientes dietarios. Gracias a que las cámaras cuentan con paneles transparentes, también se puede observar fácilmente el comportamiento animal. El sistema completo se compone de varias cámaras para respirometría y metabolismo, unidades de muestreo y análisis de gas, así como de una unidad de adquisición de datos. Tras una prueba de recuperación con metano (CH4), se observó que la tasa de recuperación de CH4 fluctuó entre 96.7 y 99.6% en 8 repeticiones, y el valor medio fue del 98,1% (coeficiente de variación = 1,3%). Un experimento preliminar con animales se llevó también a cabo con 4 cabras Coreanas negras nativas de 23.5 kg de peso. Las cabras consumieron una mezcla 50:50 de forraje y concentrado, con un suministro diario de alimento equivalente al 2% del peso corporal. La digestibilidad de la materia seca fue del 70.9%, y la producción media de CH4 fue del 11.6 g / día, correspondiente a 24.7 g / kg de consumo de materia seca. Mediante este sistema se pueden realizar con precisión y eficiencia diversos experimentos de medición de emisiones de metano, digestibilidad de nutrientes, o ambos, en pequeños rumiantes tales como cabras, ovejas y ciervos.Um curioso sistema de câmara foi desenvolvido para a medição simultânea de emissões de gás para pequenos ruminantes e a digestibilidade de nutrientes. O comportamento dos animais pode ser facilmente observado no sistema a traves de panéis transparentes. O sistema está composto de câmaras de respiração e metabolismos, unidades de amostragem análises de gás e uma unidade de aquisição de datas. Um teste de recuperação foi desenvolvido usando um gás metano estandardizado (CH4). Os valores de recuperação de CH4 variou entre 96.7 a 99.6% em 8 replicações, e a media foi de 98.1% (coeficiente de variação = 1.3%). Um experimento preliminar foi realizado utilizando 4 cabras pretas nativas coreanas com uma media de peso corporal de 23.5 kg. As cabras consumiram uma mistura de forragem e concentrado 50:50, e um suplemento do 2% do peso corporal. A digestibilidade da matéria seca foi de 70.9%, e a media da produção de gás CH4 foi de 11.6 g/dia a qual foi calculada por 24.7 g/kg do consumo de matéria seca. Usando este sistema, pesquisas com exactidão e eficiência podem ser realizadas para a medição de emissões de metano, digestibilidade de nutrientes, ou ambas em pequenos ruminantes incluindo cabras, ovelhas e veados.

Summary Background: contamination of barley with Fusarium mycotoxins causes significant economic losses for pork producers, and alleviation with mycotoxin sequestering agents has proven inconsistent. Objective: to evaluate a yeast cell wall product in preventing adverse effects of Fusarium mycotoxins on growth performance, blood characteristic, and nutrient digestibility in gilts fed diets containing barley naturally contaminated with Fusarium mycotoxins. Methods: positive and negative controls of corn-soybean meal diets containing 20% control and contaminated barley with Fusarium mycotoxins, respectively, were prepared. Two additional diets were prepared by adding 0.2 or 0.4% of a yeast cell wall product to the negative control diet. The experimental diets were fed to pigs with 61.7 Kg initial body weight for 2 weeks. Results: pigs fed the negative control diet gained less than those fed the positive control diet (p