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Your search keyword '"Bernacki, Susan H."' showing total 31 results
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31 results on '"Bernacki, Susan H."'

5. Bioelectronic sensor technology for detection of cystic fobrosis and hereditary hemochromatosis mutations

Author

Bernacki, Susan H., Farkas, Daniel H., Wenmei Shi, Chan, Vivian, Yenbou Liu, Beck, Jeanne C., Bailey, Karen Snow, Pratt, Victoria M., Monaghan, Kristin G., Matteson, Karla J., Schaefer, Frederick V., Friez, Michael, Shrimpton, Antony E., and Stenzel, Timothy T.

Subjects
Detectors -- Evaluation, Detectors -- Usage, Hemochromatosis -- Research, Cystic fibrosis -- Research
Published
2003

7. Cornifin, a cross-linked envelope in keratinocytes that is down-regulated byretinoids

Author

Marvin, Keith W., George, margaret D., Fujimoto, Wataru, Saunders, Nicholas A., Bernacki, Susan H., and Jetten, Anton M.

Subjects
Cell differentiation -- Research, Keratinocytes -- Research, Science and technology
Abstract

The cDNA clone SQ37 isolated from a rabbit squamous cell library was characterized and the function of its gene product was determined. The gene product is a protein of 14 kDa which appears to be a component of the cross-linked envelope during the later stages of squamous differentiation. SQ37 mRNA is induced during squamous differentiation of humanepidermal keratinocytes and rabbit tracheal cells, and this induction is down-regulated by retinoids. The designation proposed for the SQ37 gene productid cornifin.

Published
1992

21. Genetically Characterized Positive Control Cell Lines Derived from Residual Clinical Blood Samples

Author

Bernacki, Susan H, primary, Beck, Jeanne C, primary, Stankovic, Ana K, primary, Williams, Laurina O, primary, Amos, Jean, primary, Snow-Bailey, Karen, primary, Farkas, Daniel H, primary, Friez, Michael J, primary, Hantash, Feras M, primary, Matteson, Karla J, primary, Monaghan, Kristin G, primary, Muralidharan, Kasinathan, primary, Pratt, Victoria M, primary, Prior, Thomas W, primary, Richie, Kristy L, primary, Levin, Barbara C, primary, Rohlfs, Elizabeth M, primary, Schaefer, Frederick V, primary, Shrimpton, Antony E, primary, Spector, Elaine B, primary, Stolle, Catherine A, primary, Strom, Charles M, primary, Thibodeau, Stephen N, primary, Cole, Eugene C, primary, Goodman, Barbara K, primary, and Stenzel, Timothy T, primary

Published
2005
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23. Developing a Sustainable Process to Provide Quality Control Materials for Genetic Testing

Author

Chen, Bin, primary, O'Connell, Catherine D., additional, Boone, D Joe, additional, Amos, Jean A., additional, Beck, Jeanne C., additional, Chan, Maria M., additional, Farkas, Daniel H., additional, Lebo, Roger V., additional, Richards, Carolyn Sue, additional, Roa, Benjamin B., additional, Silverman, Lawrence M., additional, Barton, David E., additional, Bejjani, Bassem A., additional, Belloni, Dorothy R., additional, Bernacki, Susan H., additional, Caggana, Michele, additional, Charache, Patricia, additional, Dequeker, Elisabeth, additional, Ferreira-Gonzalez, Andrea, additional, Friedman, Kenneth J., additional, Greene, Carol L., additional, Grody, Wayne W., additional, Highsmith, William Edward, additional, Hinkel, Cecelia S., additional, Kalman, Lisa V., additional, Lubin, Ira M., additional, Lyon, Elaine, additional, Payne, Deborah A., additional, Pratt, Victoria M., additional, Rohlfs, Elizabeth, additional, Rundell, Clark A., additional, Schneider, Erasmus, additional, Willey, Ann M., additional, Williams, Laurina O., additional, Willey, James C., additional, Winn-Deen, Emily S., additional, and Wolff, Daynna J., additional

Published
2005
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29. Mammalian cell viability in electrospun composite nanofiber structures.

Author

Canbolat MF, Tang C, Bernacki SH, Pourdeyhimi B, and Khan S

Subjects
3T3-L1 Cells, Animals, Cell Adhesion drug effects, Cell Death drug effects, Cell Survival drug effects, Fibroblasts ultrastructure, Mice, Nanocomposites ultrastructure, Nanofibers ultrastructure, Polyesters pharmacology, Polyvinyl Alcohol pharmacology, Solutions, Solvents, Time Factors, Fibroblasts cytology, Mammals metabolism, Nanocomposites chemistry, Nanofibers chemistry, Tissue Engineering methods
Abstract

Incorporation of mammalian cells into nanofibers (cell electrospinning) and multilayered cell-nanofiber structures (cell layering) via electrospinning are promising techniques for tissue engineering applications. We investigate the viability of 3T3-L1 mouse fibroblasts after incorporation into poly(vinyl alcohol) nanofibers and multilayering with poly(caprolactone) nanofibers and analyze the possible factors that affect cell viability. We observe that cells do not survive cell electrospinning but survive cell layering. Assessing the factors involved in cell electrospinning, we find that dehydration and fiber stretching are the main causes of cell death. In cell layering, the choice of solvent is critical, as residual solvent in the electrospun fibers could be detrimental to the cells., (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2011
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30. Isolation of human mesenchymal stem cells from bone and adipose tissue.

Author

Bernacki SH, Wall ME, and Loboa EG

Subjects
Biomarkers metabolism, Cell Differentiation, Cells, Cultured, Cryopreservation methods, Humans, Mesenchymal Stem Cells metabolism, Adipose Tissue cytology, Bone and Bones cytology, Cell Culture Techniques instrumentation, Cell Culture Techniques methods, Cell Separation methods, Mesenchymal Stem Cells cytology
Published
2008
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31. Characterization of electrospun nanocomposite scaffolds and biocompatibility with adipose-derived human mesenchymal stem cells.

Author

McCullen SD, Stevens DR, Roberts WA, Clarke LI, Bernacki SH, Gorga RE, and Loboa EG

Subjects
Biocompatible Materials chemistry, Cell Culture Techniques methods, Cell Polarity, Cell Proliferation, Cell Survival, Cells, Cultured, Electrochemistry methods, Humans, Materials Testing, Osteoblasts cytology, Osteoblasts physiology, Rotation, Adipocytes cytology, Adipocytes physiology, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells physiology, Nanotubes, Carbon chemistry, Tissue Engineering methods
Abstract

Electrospun nanocomposite scaffolds were fabricated by encapsulating multi-walled carbon nanotubes (MWNT) in poly (lactic acid) (PLA) nanofibers. Scanning electron microscopy (SEM) confirmed the fabrication of nanofibers, and transmission electron microscopy identified the alignment and dispersion of MWNT along the axis of the fibers. Tensile testing showed an increase in the tensile modulus for a MWNT loading of 0.25 wt% compared with electrospun nanofibrous mats without MWNT reinforcement. Conductivity measurements indicated that the confined geometry of the fibrous system requires only minute doping to obtain significant enhancements at 0.32 wt%. Adipose-derived human mesenchymal stem cells (hMSCs) were seeded on electrospun scaffolds containing 1 wt% MWNT and 0 wt% MWNT, to determine the efficacy of the scaffolds for cell growth, and the effect of MWNT on hMSC viability and proliferation over two weeks in culture. Staining for live and dead cells and DNA quantification indicated that the hMSCs were alive and proliferating through day 14. SEM images of hMSCs at 14 days showed morphological differences, with hMSCs on PLA well spread and hMSCs on PLA with 1% MWNT closely packed and longitudinally aligned.

Published
2007

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