119 results on '"Bernay B"'
Search Results
2. A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum
- Author
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Smits, M., Artigaud, S., Bernay, B., Pichereau, V., Bargelloni, L., and Paillard, C.
- Published
- 2020
- Full Text
- View/download PDF
3. Nonlinearities of an Aircraft Piccolo Tube: Identification and Modeling
- Author
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Dossogne, T., Trillet, P., Schoukens, M., Bernay, B., Noël, J. P., Kerschen, G., Zimmerman, Kristin B., Series Editor, and Barthorpe, Robert, editor
- Published
- 2019
- Full Text
- View/download PDF
4. Behavior of Antimicrobial Peptide K4 in a Marine Environment
- Author
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Houyvet, B., Leduc, A., Cornet, V., Pontin, J., Benoist, L., Bernay, B., Henry, J., Vetois, E., and Zatylny-Gaudin, C.
- Published
- 2019
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5. Structural and functional characterization of an egg-laying hormone signaling system in a lophotrochozoan – the Pacific oyster (Crassostrea gigas)
- Author
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Favrel, P., primary, Dubos, M.P., additional, Bernay, B., additional, Pasquier, J., additional, Schwartz, J, additional, Lefranc, B., additional, Mouret, L., additional, Rivière, G., additional, Leprince, J., additional, and Bondon, A., additional
- Published
- 2023
- Full Text
- View/download PDF
6. Nonlinearities of an Aircraft Piccolo Tube: Identification and Modeling
- Author
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Dossogne, T., primary, Trillet, P., additional, Schoukens, M., additional, Bernay, B., additional, Noël, J. P., additional, and Kerschen, G., additional
- Published
- 2018
- Full Text
- View/download PDF
7. Impacts of ocean acidification and warming on post-larval growth and metabolism in two populations of the great scallop (Pecten maximusL.)
- Author
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Harney, E., primary, Rastrick, S.P.S., additional, Artigaud, S., additional, Pisapia, J., additional, Bernay, B., additional, Miner, P., additional, Pichereau, V., additional, Strand, Ø., additional, Boudry, P., additional, and Charrier, G., additional
- Published
- 2022
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8. Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity
- Author
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Mesnage, R., Bernay, B., and Séralini, G.-E.
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- 2013
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9. Inactivation of the Response Regulator AgrA Has a Pleiotropic Effect on Biofilm Formation, Pathogenesis and Stress Response in Staphylococcus lugdunensis
- Author
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Aubourg, M., Pottier, M., Leon, A., Bernay, B., Dhalluin, A., Cacaci, M., Torelli, R. (ORCID:0000-0003-1956-3981), Ledormand, P., Martini, C., Sanguinetti, M. (ORCID:0000-0002-9780-7059), Auzou, M., Gravey, F., Giard, J. -C., Aubourg, M., Pottier, M., Leon, A., Bernay, B., Dhalluin, A., Cacaci, M., Torelli, R. (ORCID:0000-0003-1956-3981), Ledormand, P., Martini, C., Sanguinetti, M. (ORCID:0000-0002-9780-7059), Auzou, M., Gravey, F., and Giard, J. -C.
- Abstract
Staphylococcus lugdunensis is a coagulase-negative Staphylococcus that emerges as an important opportunistic pathogen. However, little is known about the regulation underlying the transition from commensal to virulent state. Based on knowledge of S. aureus virulence, we suspected that the agr quorum sensing system may be an important determinant for the pathogenicity of S. lugdunensis. We investigated the functions of the transcriptional regulator AgrA using the agrA deletion mutant. AgrA played a role in cell pigmentation: DargA mutant colonies were white while the parental strains were slightly yellow. Compared with the wild-type strain, the DargA mutant was affected in its ability to form biofilm and was less able to survive in mice macrophages. Moreover, the growth of DagrA was significantly reduced by the addition of 10% NaCl or 0.4 mM H2O2 and its survival after 2 h in the presence of 1 mM H2O2 was more than 10-fold reduced. To explore the mechanisms involved beyond these phenotypes, the DagrA proteome and transcriptome were characterized by mass spectrometry and RNA-Seq. We found that AgrA controlled several virulence factors as well as stress-response factors, which are well correlated with the reduced resistance of the DagrA mutant to osmotic and oxidative stresses. These results were not the consequence of the deregulation of RNAIII of the agr system, since no phenotype or alteration of the proteomic profile has been observed for the DRNAIII mutant. Altogether, our results highlighted that the AgrA regulator of S. lugdunensis played a key role in its ability to become pathogenic.
- Published
- 2022
10. PHARMACOLOGICAL INHIBITION OF EZH2 SUPPRESSES PAIN IN MICE WITH MONOIODOACETATE-INDUCED OSTEOARTHRITIS BY REGULATING INFLAMMATION, OXIDATIVE STRESS, AND AUTOPHAGY IN SYNOVIAL CELLS.
- Author
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Brochard, S., primary, Aury-Landas, J., additional, Agin, V., additional, Bernay, B., additional, Pontin, J., additional, Seillier, C., additional, Toutirais, O., additional, Cohen-Solal, M., additional, Hay, E., additional, Boumediene, K., additional, and Baugé, C., additional
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- 2022
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11. Proteomic study of inner and outer hen egg vitelline membranes: insights into the biological functions of vitelline membrane layers
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Brégeon, Mégane, Bernay, B., Labas, Valérie, Chessé, Magali, Zatylny-Gaudin, Céline, Réhault-Godbert, Sophie, Guyot, Nicolas, and REHAULT-GODBERT, Sophie
- Subjects
[SDV] Life Sciences [q-bio] ,proteomics ,protein identification ,protein function ,hen egg ,outer/inner vitelline membrane - Abstract
The hen egg vitelline membrane (VM) is a proteinaceous membrane separating the egg white from the yolk. The VM is involved in fertilization, in early stages of embryogenesis but also in protection of the embryo. It consists of the inner layer (IVM) and the outer layer (OVM), that are in contact with the yolk and the egg white, respectively. These two layers are synthesized by distinct tissues namely the ovary/liver for IVM and the oviduct for OVM. About 140 different proteins were identified in the VM to date; however, the distribution of most of these proteins between IVM and OVM remains to be elucidated. The VM contains several ZP proteins that are likely to be involved in sperm-oocyte interactions during fertilization. VM is also crucial for embryonic development as it provides a substratum for embryonic and yolk sac expansion in early incubation. Moreover, VM is a physical barrier protecting the embryo from the egg white alkalinity and from potential microbial contaminations, and the high amount of antimicrobials in VM (lysozyme, AvBD11...) also contributes to the antimicrobial defense of the developing embryo. Thus, VM plays a key role, not only in fertilization, but also during incubation. The present study aims at determining the protein composition of IVM and OVM to identify the intrinsic putative functions of each VM layer. IVM and OVM were manually separated from the VM of freshly-laid eggs and proteins from these two layers were independently solubilized, and analyzed by SDS-PAGE and GeLC-MS/MS. More than 550 proteins were identified with two peptides in the whole VM. Around 440 and 380 proteins were identified in IVM and OVM, respectively. Preliminary data resulting from the functional annotation of proteins (gene ontology/bibliography) revealed the presence of many proteins putatively associated with cell adhesion and migration, in addition to proteins involved in fertilization and antimicrobial defense. Altogether, the data generated from this study will give new insights into the structure and composition of the chicken egg VM and will provide an integrative overview of the respective physiological functions of IVM and OVM. Next, it will be interesting to further study how storage conditions prior to incubation may alter the structural/molecular integrity of IVM and OVM, and consequently negatively or positively impact the revival of embryonic development during incubation.
- Published
- 2019
12. A proteomic study of resistance to brown ring disease in the manila clam, Ruditapes philippinarum
- Author
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Smits, M., primary, Artigaud, S., additional, Bernay, B., additional, Bidault, A., additional, Bargelloni, L., additional, and Paillard, C., additional
- Published
- 2019
- Full Text
- View/download PDF
13. Behavior of Antimicrobial Peptide K4 in a Marine Environment
- Author
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Houyvet, B., primary, Leduc, A., additional, Cornet, V., additional, Pontin, J., additional, Benoist, L., additional, Bernay, B., additional, Henry, J., additional, Vetois, E., additional, and Zatylny-Gaudin, C., additional
- Published
- 2018
- Full Text
- View/download PDF
14. Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve, Pecten maximus (Linnaeus, 1758)
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Boonmee, A., Berthelin, C. Heude, Kingtong, S., Pauletto, M., Bernay, B., Adeline, B., Suquet, Marc, Sourdaine, P., Kellner, K., Boonmee, A., Berthelin, C. Heude, Kingtong, S., Pauletto, M., Bernay, B., Adeline, B., Suquet, Marc, Sourdaine, P., and Kellner, K.
- Abstract
In order to investigate the mechanisms of final maturation and capacitation of spermatozoa in Pecten maximus, we used a 2D proteomic approach coupled with MALDI-TOF/TOF mass spectrometry (MS) and bioinformatics search against the Pecten database, to set up a reference map of the proteome of spawned spermatozoa, and identified 133 proteins on the basis of the EST database. These proteins are mainly involved in energy production, ion and electron transport (44%), cell movement (22%) and developmental processes (10%). Comparison between proteomes of spermatozoa collected before and after transit through the genital ducts of P. maximus led to the identification of differentially expressed proteins. Most of them are associated with energy metabolism (aconitate hydratase, malate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase), indicating important modifications of energy production during transit in gonoducts, potentially linked with acquisition of sperm motility. Three proteins involved in cell movement (Tektin-2, tubulin and microtubule-associated protein RP/EB family member 3) were down-regulated in spermatozoa stripped from the gonad. 40S ribosomal protein SA, involved in maturation of 40S ribosomal subunits, was also found to be down-regulated in spermatozoa obtained by induced spawning, suggesting reduction of the efficiency of RNA translation, a characteristic of late spermatozoon differentiation. These results confirm that maturation processes of P. maximus spermatozoa during transit through the gonoduct involve RNA translation, energy metabolism and structural proteins implicated in cell movement. Spermatozoa maturation processes clearly differ between P. maximus and gonochoric or alternately hermaphroditic bivalves, potentially in relation to reproductive strategies: the final maturation of the spermatozoon along the genital tract probably contributes to reduction of autofertilization in this simultaneously hermaphroditic species.
- Published
- 2016
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15. Effect of simulated air dive and decompression sickness on the plasma proteome of rats
- Author
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Lautridou, J., Pichereau, V., Artigaud, S., Buzzacott, Peter, Wang, Q., Bernay, B., Driad, S., Mazur, A., Lambrechts, K., Théron, M., Guerrero, F., Lautridou, J., Pichereau, V., Artigaud, S., Buzzacott, Peter, Wang, Q., Bernay, B., Driad, S., Mazur, A., Lambrechts, K., Théron, M., and Guerrero, F.
- Abstract
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Purpose: Decompression sickness (DCS) is a poorly understood systemic disease caused by inadequate desaturation following a reduction in ambient pressure. Although recent studies highlight the importance of circulating factors, the available data are still puzzling. In this study, we aimed to identify proteins and biological pathways involved in the development of DCS in rats. Experimental design: Eighteen male Sprague-Dawley rats were subjected to a same simulated air dive to 1000 kPa absolute pressure and divided into two groups: no DCS or DCS. A third control group remained at atmospheric pressure. Venous blood was collected after hyperbaric exposure and the plasma proteomes from four individuals per group were analyzed by using a two-dimensional electrophoresis-based proteomic strategy. Results: Quantitative analysis identified nine protein spots with abundances significantly changed (false discovery rate < 0.1) between the tested conditions. Three protein spots, identified as Apolipoprotein A1, Serine Protease Inhibitor A3K (Serpin A3K), and Alpha-1-antiproteinase, appeared increased in DCS animals but displayed only weak changes. By contrast, one protein spot identified as Transthyretin (TTR) dramatically decreased (i.e. quite disappeared) in animals displaying DCS symptoms. Before diving, TTR level was not different in DCS than nondiving group. Conclusion: These results may lead to the use of TTR as an early biomarker of DCS.
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- 2016
16. Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve,Pecten maximus(Linnaeus, 1758)
- Author
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Boonmee, A., primary, Heude Berthelin, C., additional, Kingtong, S., additional, Pauletto, M., additional, Bernay, B., additional, Adeline, B., additional, Suquet, M., additional, Sourdaine, P., additional, and Kellner, K., additional
- Published
- 2016
- Full Text
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17. 2010001M06rik (St102) protects striatal neurons against an N-terminal fragmentof mutant huntingtin in vivo
- Author
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Brouillet, E., Galvan, Laurie, Gaillard, M.-C, Lepejovà, N, Petit, F, Francelle, L., Malgorn, C, Bernay, B., Guillermier, M., Escartin, C, Bonvento, G., Dufour, N., Elalouf, J.-M, Hantraye, P., de Chaldee, M., Deglon, N., Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), University of California [Los Angeles] (UCLA), University of California (UC), Service MIRCEN (MIRCEN), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Géosciences Marines (GM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Maladies Neurodégénératives - UMR 9199 (LMN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire de la neurotransmission et des processus neurodégénératifs (LGMNPN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie et de Technologies de Saclay (IBITECS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, University of California, Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), IFREMER, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), and Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
- Subjects
nervous system ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] - Abstract
International audience; The mechanisms underlying the preferential vulnerability of striatal neurons to mutant Huntingtin (mHtt) in Huntington's disease (HD) remain unknown. Our hypothesis, supported by recent publications, is that genes selectively expressed in the striatum may play a role in this susceptibility to mHtt. In the present study we focused on a product of the 2010001M06rik (St102) gene, previously identified based on its preferential expression in the striatum and its significantly reduced levels in the striatum of R6/2 mice (Brochier et al., Physiol Genomics, 2008). We examined whether modifying St102 expression could change the neurotoxic effects of an N-terminal fragment of mHtt expressed in the mouse striatum using the HD lentiviral model (LV-Htt171-82Q). We developed lentiviral vectors to overexpress St102 (LV-St102) or knock-down St102 using a selective shRNA (LV-shRNA-St102). RT-PCR analysis infection of the striatum of adult mice with LV-St102 or LV-shRNA-St102 led to a significant increase or decrease of St102 expression respectively, without producing overt alterations as assessed using immunohistochemistry (IHC) of NeuN and DARPP32. LV-St102 and LV-shRNA-St102 were co-injected with LV-Htt171-82Q in the striatum of adult WT mice. Six weeks after injections, LV-Htt171-82Q consistently produced striatal lesions characterized by a loss of NeuN and DARPP32. Interestingly, the co-expression of Htt171-82Q and shRNA-St102 led to a significant increase in the lesion volume. On the contrary, the co-expression of Htt171-82Q and St102 overexpression led to a significant decrease of the lesion size. These results suggest that the loss of St102 expression could play a role in striatal degeneration in HD.
- Published
- 2012
18. Branch and Price for a reliability oriented DARP model
- Author
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Deleplanque, Samuel, Quilliot, Alain, Bernay, B., Deleplanque, Samuel, Quilliot, Alain, and Bernay, B.
- Abstract
info:eu-repo/semantics/published
- Published
- 2014
19. Routing on dynamic networks: GRASP versus genetic
- Author
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Bernay, B., Deleplanque, Samuel, Quilliot, Alain, Bernay, B., Deleplanque, Samuel, and Quilliot, Alain
- Abstract
info:eu-repo/semantics/published
- Published
- 2014
20. PHARMACOLOGICAL INHIBITION OF EZH2 SUPPRESSES PAIN IN MICE WITH MONOIODOACETATE-INDUCED OSTEOARTHRITIS BY REGULATING INFLAMMATION, OXIDATIVE STRESS, AND AUTOPHAGY IN SYNOVIAL CELLS
- Author
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Brochard, S., Aury-Landas, J., Agin, V., Bernay, B., Pontin, J., Seillier, C., Toutirais, O., Cohen-Solal, M., Eric Hay, Boumediene, K., and Bauge, C.
- Subjects
Rheumatology ,Biomedical Engineering ,Orthopedics and Sports Medicine
21. L'inhibition d'EZH2 améliore l'arthrose et la douleur articulaire en réduisant l'inflammation des cellules synoviales et des macrophages, le guidage des axones et l'ostéoclastogenèse.
- Author
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Brochard, S., Taieb, M., Richard, I., Aury-Landas, J., Bernay, B., Agin, V., Boumediene, K., and Bauge, C.
- Abstract
Précédemment, nous avons démontré que l'inhibition d'EZH2 réduit la progression de l'arthrose dans un modèle murin d'arthrose induit par chirurgie et régule l'inflammation et le catabolisme dans les chondrocytes. Ici, nous avons étudié son effet dans un autre modèle d'arthrose expérimental et investigué le mécanisme d'action sur les autres cellules impliquées dans la pathologie. L'arthrose a été induite par injection intra-articulaire de MIA dans des souris. La progression de la pathologie est évaluée par histologie et la douleur articulaire par des tests comportementaux. In vitro, des synoviocytes et des cellules de moelle osseuses ont été obtenus à partir de têtes fémorales de patients arthrosiques. Les synoviocytes ont été stimulés à l'interleukine-1, en présence ou non d'un inhibiteur pharmacologique d'EZH2, l'EPZ6438 (aussi appelé Tazemetostat). Les cibles d'EZH2 ont été recherchées par Chip-Seq et analyse protéomique. Finalement, les effets de l'inhibiteur ont été testés sur la différenciation des macrophages et la formation des ostéoclastes. Ici, nous montrons que l'inhibition d'EZH2 a réduit la progression de l'arthrose et la douleur dans le modèle expérimental murin d'arthrose. L'IL-1b a augmenté l'expression d'EZH2 dans les synoviocytes. Dans ces cellules, l'inhibiteur d'EZH2 a réduit l'expression des gènes impliqués dans l'inflammation, la douleur et le catabolisme et a augmenté l'autophagie. Les expériences de protéomique confirment que l'inhibition d'EZH2 affecte l'expression de protéines impliquées dans la signalisation de l'IL-1, l'activation des MMP et l'autophagie, et montrent également une dérégulation des protéines impliquées dans le « métabolisme » et le « guidage axonal ». Par ailleurs, nous montrons que l'inhibition d'EZH2 a réduit la polarisation M1 des macrophages, et la formation des ostéoclastes, cellules connues pour leur implication dans la douleur articulaire. En conclusion, cette étude montre l'importance de l'histone méthyl-transférase EZH2 dans la physiopathologie de l'arthrose et la douleur articulaire. Mécanistiquement, EZH2 agit sur l'ensemble des tissus impliqués dans la survenue de cette pathologie complexe : cartilage, os, synovial, faisant de l'inhibition d'EZH2 une stratégie thérapeutique prometteuse pour le traitement de cette maladie grave. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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22. Calciprotein particle-induced calcium overload triggers mitochondrial dysfunction in endothelial cells.
- Author
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Feenstra L, Chatre L, Bernay B, Pontin J, Mastik MF, van Buiten A, Nakládal D, Star BS, Hillebrands JL, and Krenning G
- Abstract
Calciprotein particles (CPPs) are calcium- and phosphate-containing nanoparticles numbers of which are increased in patients with chronic kidney disease (CKD). CPPs have been associated with the development of vascular disease, although the underlying mechanisms are unknown. We previously showed that CPPs induce endothelial cell (EC) dysfunction by reducing nitric oxide (NO) bioavailability and generating superoxide (O
2 .- ). Here, we tested the hypothesis that CPPs induce mitochondrial calcium (Ca2+ ) overload, which may trigger mitochondrial dysfunction and, consequently, EC activation. Exposure of human umbilical vein ECs to CPPs resulted in significantly increased cytosolic and mitochondrial Ca2+ levels compared to vehicle-treated ECs. Proteome analysis demonstrated impaired endoplasmic reticulum calcium signalling, and decreased enrichment of proteins in the mitochondrial OXPHOS complexes I-III in CPP-exposed ECs. Respirometry data confirmed these findings and demonstrated decreased basal and maximal respiration in CPP-exposed ECs. This was accompanied by reduced mitochondrial membrane potential, reduced antioxidant capacity and loss of mitochondria. In the presence of cyclosporin A, a potent mitochondrial permeability transition pore inhibitor, CPP-induced EC activation and cell death were attenuated. Taken together, our data indicate that CPP-induced Ca2+ overload is an important trigger of mitochondrial dysfunction, and EC activation and cell loss, which eventually may contribute to the development of vascular diseases in CKD. Interventions that target CPP-induced mitochondrial dysfunction might preserve EC function and possibly alleviate the development of vascular diseases in CKD. KEY POINTS: Calciprotein particles (CPPs) are calcium- and phosphate-containing nanoparticles numbers of which are increased in patients with chronic kidney disease and which have been associated with the development of vascular disease. In this study, we tested the hypothesis that CPPs induce mitochondrial calcium (Ca2+ ) overload in endothelial cells, thereby triggering mitochondrial dysfunction and endothelial activation. We show that exposure of HUVECs (human umbilical vein endothelial cells) to CPPs results in increased cytosolic and mitochondrial Ca2+ levels, which is associated with alterations in mitochondrial processes (proteome analysis), cellular respiration, mitochondrial integrity and number. CPP-induced EC activation and cell death were attenuated in the presence of cyclosporin A, a potent mitochondrial permeability transition pore inhibitor. Our data indicate that CPP-induced Ca2+ overload triggers mitochondrial dysfunction, endothelial activation and cell loss. Interventions that target CPP-induced mitochondrial dysfunction might preserve EC function in chronic kidney disease., (© 2025 The Author(s). The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.)- Published
- 2025
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23. Multi-omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae.
- Author
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Barreda L, Brosse C, Boutet S, Klewko N, De Vos D, Francois T, Collet B, Grain D, Boulard C, Totozafy JC, Bernay B, Perreau F, Lepiniec L, Rajjou L, and Corso M
- Subjects
- Transcriptome, Gene Expression Regulation, Plant, Arabidopsis metabolism, Arabidopsis genetics, Germination, Plant Proteins metabolism, Plant Proteins genetics, Brassica napus metabolism, Brassica napus genetics, Multiomics, Seeds metabolism, Seeds genetics, Brassicaceae metabolism, Brassicaceae genetics, Glucosinolates metabolism, Metabolomics, Proteomics
- Abstract
Seeds of Brassicaceae produce a large diversity of beneficial and antinutritional specialized metabolites (SMs) that influence their quality and provide resistance to stresses. While SM distribution has been described in leaves and root tissues, limited information is available about their spatiotemporal accumulation in seeds. Camelina sativa (camelina) is an oilseed Brassicaceae cultivated for human and animal nutrition and for industrial uses. While we previously explored SM diversity and plasticity, no information is available about SM distribution and expression of related proteins and genes in camelina seeds. In this study, we used a multi-omic approach, integrating untargeted metabolomics, proteomics, and transcriptomics to investigate the synthesis, modification, and degradation of SMs accumulated in camelina seed tissues (seed coat, endosperm, embryo) at six developmental and two germination stages. Metabolomic results showed distinct patterns of SMs and their related pathways, highlighting significant contrasts in seed composition and spatial distribution for the defense-related and antinutritional glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana, and Brassica napus, three closely related Brassicaceae species. Notably, thanks to metabolomic and proteomic/transcriptomic techniques the variation in GSL spatial distributions was primarily driven by differences in their structure (metabolomics data) and transport (transcriptomic and proteomic data) mechanisms. Long-chain C8-C11 methylsulfinylalkyl GSLs were predominantly accumulated in the seed coat and endosperm, while mid- and short-chain C3-C7 methylsulfinylalkyl GSLs were accumulated in the embryo. Characterizing the spatial dynamics of seed SMs provides valuable insights that can guide the development of crops with optimized distribution of beneficial and toxic metabolites, improving seed nutritional profiles., (© 2025 Society for Experimental Biology and John Wiley & Sons Ltd.)
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- 2025
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24. Phospho-Proteomics Analysis of Early Response to X-Ray Irradiation Reveals Molecular Mechanism Potentially Related to U251 Cell Radioresistance.
- Author
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Ben Diouf O, Gilbert A, Bernay B, Syljuåsen RG, Tudor M, Temelie M, Savu DI, Soumboundou M, Sall C, and Chevalier F
- Abstract
Glioblastoma (GBM) is a devastating malignant brain tumor with a poor prognosis. GBM is associated with radioresistance. Post-translational modifications (PTMs) such as protein phosphorylation can play an important role in the cellular response to radiation. To better understand the early cellular activities after radiation in GBM, we carried out a phospho-proteomic study on the U251 cell line 3 h after X-ray irradiation (6Gy) and on non-irradiated cells. Our study showed a strong modification of proteoform phosphorylation in response to radiation. We found 453 differentially expressed phosphopeptides (DEPs), with 211 being upregulated and 242 being downregulated. A GO enrichment analysis of DEPs showed a strong enrichment of the signaling pathways involved in DNA damage response after irradiation and categorized them into biological processes (BPs), cellular components (CCs) and molecular functions (MFs). Certain accessions such as BRCA1, MDC1, H2AX, MDC1, TP53BP1 were dynamically altered in our fraction and are highly associated with the signaling pathways enriched after radiation.
- Published
- 2024
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25. A mass spectrometry-based peptidomic dataset of the spermosphere in common bean (Phaseolus vulgaris L.) seeds.
- Author
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Saccaram C, Brosse C, Collet B, Sourdeval D, François T, Bernay B, Corso M, and Rajjou L
- Subjects
- Tandem Mass Spectrometry, Plant Proteins, Chromatography, Liquid, Germination, Phaseolus chemistry, Seeds chemistry, Peptides
- Abstract
The spermosphere, a dynamic microenvironment surrounding germinating seeds, is shaped by the complex interactions between natural compounds exuded by seeds and seed-associated microbial communities. While peptides exuded by plants are known to influence microbiota diversity, little is known about those specifically exuded by seeds. In this study, we characterised the peptidome profile of the spermosphere for the first time using seeds from eight genotypes of common bean (Phaseolus vulgaris) grown in two contrasting production regions. An untargeted LC-MS/MS peptidomic analysis revealed 3,258 peptides derived from 414 precursor proteins of common bean in the spermosphere. This comprehensive peptidomic dataset provides valuable insights into the characteristics of peptides exuded by common bean seeds in the spermosphere. It can be used to identify peptides with potential antimicrobial or other biological activities, advancing our understanding of the functional roles of seed-exuded peptides in the spermosphere., (© 2024. The Author(s).)
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- 2024
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26. Ionomic and proteomic changes highlight the effect of silicon supply on the nodules functioning of Trifolium incarnatum L.
- Author
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Coquerel R, Arkoun M, Trouverie J, Bernay B, Laîné P, and Etienne P
- Abstract
Introduction: Numerous studies have reported the beneficial effects of silicon (Si) in alleviating biotic or abiotic stresses in many plant species. However, the role of Si in Fabaceae facing environmental stress is poorly documented. The aim of this study is to investigate the effect of Si on physiological traits and nodulation efficiency in Trifolium incarnatum L., Methods: Si was supplied (1.7 mM in the form of Na
2 SiO3 ) plants inoculated with Rhizobium leguminosarum bv trifolii and plant physiological traits and nodule ionomic and molecular traits were monitored over 25 days., Results: Si supply promoted shoot biomass, the quantity of both Si and N in roots and shoots, and the number, biomass and density of nodules and their nitrogenase abundance which contribute to better dinitrogen (N2 ) fixation. Ionomic analysis of nodules revealed that Si supply increased the amount of several macroelements (potassium, phosphorus and sulfur) and microelements (copper, zinc and molybdenum) known to improve nodulation efficiency and N2 fixation. Finally, comparative proteomic analysis (+Si versus -Si) of nodules highlighted that Si modulated the proteome of both symbionts with 989 and 212 differentially accumulated proteins (DAPs) in the infected host root cells and their symbiont bacteria, respectively., Discussion: Among the DAPs, the roles of those involved in nodulation and N2 fixation are discussed. For the first time, this study provides new insights into the effects of Si on both nodular partners and paves the way for a better understanding of the impact of Si on improving nodule function, and more specifically, on the nodules' N2 -fixing capacity., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Coquerel, Arkoun, Trouverie, Bernay, Laîné and Etienne.)- Published
- 2024
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27. Characterization of gonadotropins and their receptors in a chondrichthyan, Scyliorhinus canicula, fills a gap in the understanding of their coevolution.
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Jeanne F, Pilet S, Klett D, Combarnous Y, Bernay B, Dufour S, Favrel P, and Sourdaine P
- Subjects
- Animals, Receptors, FSH genetics, Receptors, FSH metabolism, Receptors, LH genetics, Receptors, LH metabolism, Female, Male, Humans, Luteinizing Hormone metabolism, Phylogeny, Follicle Stimulating Hormone metabolism, Gonadotropins metabolism, Gonadotropins genetics, Receptors, Gonadotropin metabolism, Receptors, Gonadotropin genetics
- Abstract
In Gnathostomes, reproduction is mainly controlled by the hypothalamic-pituitary-gonadal (HPG) axis, with the involvement of the pituitary gonadotropic hormones (GTH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which activate their cognate receptors, FSHR and LHR, expressed in gonads. Each GTH consists of a common α subunit and of a specific FSHβ or LHβ subunit. Chondrichthyes (holocephalans and elasmobranchs) is a sister group of bony vertebrates. This position is highly favorable for the understanding of the evolution of endocrine regulations of reproduction among gnathostomes. Surprisingly, the characterization of gonadotropins and their receptors is still limited in chondrichthyes. In the present study, GTH and GTHR sequences have been identified from several chondrichthyan genomes, and their primary structures were analyzed relative to human orthologs. 3D models of GTH/GTHR interaction were built, highlighting the importance of the receptor hinge region for ligand recognition. Functional hormone-receptor interactions have been studied in HEK cells using the small-spotted catshark (Scyliorhinus canicula) recombinant proteins and showed that LHR was specifically activated by LH whereas FSHR was activated by both FSH and LH. Expression profiles of GTHs and their receptors were explored by real-time PCR, in situ hybridization and immunohistochemistry during spermatogenesis, along the male genital tract and other tissues, as well as in some female tissues for comparison. Tissue-expression analyses showed that the highest levels were observed for fshr transcripts in testis and ovary and for lhr in specific extragonadal tissues. The two receptors were expressed at all stages of spermatogenesis by both germ cells and somatic cells, including undifferentiated spermatogonia, spermatocytes, spermatids, somatic precursors and Sertoli cells; differentiated Leydig cells being absent in the testis of S. canicula. Receptors were also expressed by the lymphomyeloid epigonal tissue and the testicular tubules. These results, suggest a wide range of gonadotropin-regulated functions in Elasmobranchs, as well as functional redundancy during spermatogenesis. These extended functions are discussed in an evolutionary context in which the specificity of gonadotropin signaling must have contributed to the evolution of gonadal cells' morphology and function., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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- 2024
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28. Specific and Polyfunctional T Cell Response Against N -Methyl-d-aspartate Receptor in an Autoantibody-Mediated Encephalitis Model.
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Lesec L, Serrier J, Seillier C, Bernay B, Regnauld C, Furon J, Leprince J, Lefranc B, Vivien D, Docagne F, Le Mauff B, and Toutirais O
- Abstract
Background: Anti- N -Methyl-d-aspartate receptor (NMDAR) autoimmune encephalitis (NMDAR AE) is an autoimmune disease characterized by severe psychiatric and neurological symptoms. While the pathogenic role of antibodies (Abs) directed against the GluN1 subunit of NMDAR is well described in this disease, the immune mechanisms involved in the generation of the autoimmune B cell response, especially the role of T helper cells, are poorly understood. Previously, we developed a B-cell-mediated mouse model of NMDAR AE by immunization with a GluN1
359-378 peptide that drives a series of symptoms that recapitulate AE such as anxiety behaviour and spatial memory impairment., Results: In this mouse model, we identified anti-GluN1-specific CD4+ but also CD8+ T cells in both spleen and meninges. T helper cells have a polyfunctional profile, arguing for a T and B cell crosstalk to generate anti-GluN1 pathogenic Abs. Interestingly, proteomic analysis of AE meninges showed enrichment of differentially expressed proteins in biological processes associated with B cell activation and cytokine signalling pathways., Conclusions: This study identified, for the first time, a potential contribution of T helper cells in the pathology of NMDAR AE and paved the way for the development of future tolerogenic approaches to treat relapses.- Published
- 2024
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29. Nicotinamide riboside modulates the reactive species interactome, bioenergetic status and proteomic landscape in a brain-region-specific manner.
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Marmolejo-Garza A, Chatre L, Croteau DL, Herron-Bedoya A, Luu MDA, Bernay B, Pontin J, Bohr VA, Boddeke E, and Dolga AM
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- Animals, Mice, Proteomics, Proteome metabolism, Proteome drug effects, Oxidative Stress drug effects, Oxidative Stress physiology, Mice, Inbred C57BL, Male, Reactive Oxygen Species metabolism, Hippocampus metabolism, Hippocampus drug effects, Neurons metabolism, Neurons drug effects, Niacinamide analogs & derivatives, Niacinamide pharmacology, Pyridinium Compounds, Brain metabolism, Brain drug effects, Alzheimer Disease metabolism, Energy Metabolism drug effects, Energy Metabolism physiology
- Abstract
Nicotinamide riboside (NR), a precursor of nicotinamide adenine dinucleotide (NAD+), has robust cognitive benefits and alleviates neuroinflammation in Alzheimer's Disease (AD) mouse models without decreasing beta-amyloid plaque pathology. Such effects may be mediated by the reactive species interactome (RSI), at the metabolome level. In this study, we employed in vitro and in vivo models of oxidative stress, aging and AD to profile the effects of NR on neuronal survival, RSI, and the whole proteome characterization of cortex and hippocampus. RSI analysis yielded a complex modulation upon NR treatment. We constructed protein co-expression networks and correlated them to NR treatment and all measured reactive species. We observed brain-area specific effects of NR on co-expressed protein modules of oxidative phosphorylation, fatty acid oxidation, and neurotransmitter regulation pathways, which correlated with RSI components. The current study contributes to the understanding of modulation of the metabolome, specifically after NR treatment in AD and how it may play disease-modifying roles., Competing Interests: Declaration of competing interest V.A.B. has CRADA arrangements with ChromaDex. All other authors declare no competing interests., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2024
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30. Reproductive aging weakens offspring survival and constrains the telomerase response to herpesvirus in Pacific oysters.
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Dupoué A, Koechlin H, Huber M, Merrien P, Le Grand J, Corporeau C, Fleury E, Bernay B, de Villemereuil P, Morga B, and Le Luyer J
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- Animals, Telomere metabolism, Telomere genetics, Herpesviridae physiology, Female, Telomere Homeostasis, Ostreidae virology, Telomerase metabolism, Telomerase genetics, Reproduction, Aging
- Abstract
Telomere length (TL) is increasingly recognized as a molecular marker that reflects how reproductive aging affects intergenerational transmissions. Here, we investigated the effects of parental age on offspring survival and the regulation of TL by examining the telomere-elongating activity of telomerase in the Pacific oyster. We assessed the classical hallmarks of aging in parents at three age classes (young, middle-aged, and old) and crossbred them using a split-brood design to examine the consequences of the nine maternal-by-paternal age combinations on their offspring. Reproductive aging leads to increased larval mortality and accelerated telomere shortening in spats, rendering them more susceptible to infection by the Ostreid herpesvirus. Viral exposure stimulates telomerase activity, a response that we identified as adaptive, but weakened by parental aging. While telomerase lengthens a spat's telomere, paradoxically, longer individual TL predicts higher mortality in adults. The telomerase-telomere complex appeared as a conservative biomarker for distinguishing survivors and losers upon exposure to polymicrobial diseases.
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- 2024
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31. MRI-based microthrombi detection in stroke with polydopamine iron oxide.
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Jacqmarcq C, Picot A, Flon J, Lebrun F, Martinez de Lizarrondo S, Naveau M, Bernay B, Goux D, Rubio M, Malzert-Fréon A, Michel A, Proamer F, Mangin P, Gauberti M, Vivien D, and Bonnard T
- Subjects
- Animals, Mice, Male, Stroke diagnostic imaging, Humans, Fibrinogen metabolism, Ischemic Stroke diagnostic imaging, Mice, Inbred C57BL, Protein Corona chemistry, Protein Corona metabolism, Brain diagnostic imaging, Brain metabolism, Brain pathology, Polymers chemistry, Magnetic Resonance Imaging methods, Indoles chemistry, Contrast Media chemistry, Ferric Compounds chemistry, Disease Models, Animal, Thrombosis diagnostic imaging
- Abstract
In acute ischemic stroke, even when successful recanalization is obtained, downstream microcirculation may still be obstructed by microvascular thrombosis, which is associated with compromised brain reperfusion and cognitive decline. Identifying these microthrombi through non-invasive methods remains challenging. We developed the PHySIOMIC (Polydopamine Hybridized Self-assembled Iron Oxide Mussel Inspired Clusters), a MRI-based contrast agent that unmasks these microthrombi. In a mouse model of thromboembolic ischemic stroke, our findings demonstrate that the PHySIOMIC generate a distinct hypointense signal on T
2 *-weighted MRI in the presence of microthrombi, that correlates with the lesion areas observed 24 hours post-stroke. Our microfluidic studies reveal the role of fibrinogen in the protein corona for the thrombosis targeting properties. Finally, we observe the biodegradation and biocompatibility of these particles. This work demonstrates that the PHySIOMIC particles offer an innovative and valuable tool for non-invasive in vivo diagnosis and monitoring of microthrombi, using MRI during ischemic stroke., (© 2024. The Author(s).)- Published
- 2024
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32. Evaluating the toxicity of estetrol, 17α-ethinylestradiol, and their combination with drospirenone on zebrafish larvae: A behavioural and proteomic study.
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Baekelandt S, Leroux N, Lambert J, Bernay B, Robert JB, Burattin L, Gérard C, Delierneux C, Cornet V, and Kestemont P
- Subjects
- Animals, Embryo, Nonmammalian drug effects, Zebrafish, Ethinyl Estradiol toxicity, Water Pollutants, Chemical toxicity, Androstenes toxicity, Proteomics, Behavior, Animal drug effects, Larva drug effects
- Abstract
Objective: To characterise and compare the toxicity of estetrol (E4) and 17α-ethinylestradiol (EE2), and their respective mixture with the progestin drospirenone (DRSP) in zebrafish (Danio rerio) embryos., Methods: Zebrafish embryos were exposed to E4, EE2, DRSP, E4+DRSP, and EE2+DRSP in a fish embryo acute toxicity (FET) test. A second test examined behavioural responses and, using label-free proteomics, identified changes in protein expression in response to hormonal treatments, across a range of concentrations, including those that are considered to be environmentally relevant., Results: In the FET test, no effects were found from E4 at concentrations ≤100 mg/L, while EE2 induced mortality and morphological abnormalities at concentrations of 1-2 mg/L. In the behavioural test, exposure to 30 ng/L EE2 (∼200 × predicted environmental concentration - PEC) resulted in hypoactivity in fish larvae and exposure to 0.3 ng/L EE2 (∼2 × PEC) led to quantitative changes in protein abundance, revealing potential impacts on RNA processing and protein synthesis machinery. Exposure to E4 did not alter behaviour, but several groups of proteins were modulated, mainly at 710 ng/L (∼200 × PEC), including proteins involved in oxidative phosphorylation. When combined with DRSP, EE2 induced reduced effects on behaviour and proteomic responses, suggesting an antagonistic effect of DRSP. E4+DRSP induced no significant effects on behaviour or proteomic profiles at tested concentrations., Conclusions: These findings suggest that E4-based combined oral contraceptives present a more favourable environmental profile than EE2-based contraceptives, particularly during the early developmental stages of fish., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Céline Gérard and Céline Delierneux are employees of Mithra Pharmaceuticals., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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33. Proteomic characterization of persisters in Enterococcus faecium.
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Pont CL, Bernay B, Gérard M, Dhalluin A, Gravey F, and Giard JC
- Subjects
- Humans, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Proteomics, Ciprofloxacin pharmacology, Recurrence, Microbial Sensitivity Tests, Enterococcus faecium genetics
- Abstract
Background: Enterococcus faecium is a Gram-positive bacterium, naturally present in the human intestinal microbiota, but is also an opportunistic pathogen responsible for healthcare-associated infections. Persisters are individuals of a subpopulation able to survive by arrest of growth coping with conditions that are lethal for the rest of the population. These persistent cells can grow again when the stress disappears from their environment and can cause relapses., Results: In this study, we highlighted that ciprofloxacin (10-fold the MIC) led to the formation of persister cells of E. faecium. The kill curve was typically biphasic with an initial drop of survival (more than 2 orders of magnitude reduction) followed by a constant bacterial count. Growth curves and antimicrobial susceptibility tests of these persisters were similar to those of the original cells. In addition, by genomic analyses, we confirmed that the persisters were genotypically identical to the wild type. Comparative proteomic analysis revealed that 56 proteins have significantly different abundances in persisters compared to cells harvested before the addition of stressing agent. Most of them were related to energetic metabolisms, some polypeptides were involved in transcription regulation, and seven were stress proteins like CspA, PrsA, ClpX and particularly enzymes linked to the oxidative stress response., Conclusions: This work provided evidences that the pathogen E. faecium was able to enter a state of persister that may have an impact in chronic infections and relapses. Moreover, putative key effectors of this phenotypical behavior were identified by proteomic approach., (© 2023. The Author(s).)
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- 2024
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34. Antibiofilm and Antivirulence Properties of 6-Polyaminosteroid Derivatives against Antibiotic-Resistant Bacteria.
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Vergoz D, Le H, Bernay B, Schaumann A, Barreau M, Nilly F, Desriac F, Tahrioui A, Giard JC, Lesouhaitier O, Chevalier S, Brunel JM, Muller C, and Dé E
- Abstract
The emergence of multi-drug resistant pathogens is a major public health problem, leading us to rethink and innovate our bacterial control strategies. Here, we explore the antibiofilm and antivirulence activities of nineteen 6-polyaminosterol derivatives (squalamine-based), presenting a modulation of their polyamine side chain on four major pathogens, i.e., carbapenem-resistant A. baumannii (CRAB) and P. aeruginosa (CRPA), methicillin-resistant S. aureus (MRSA), and vancomycin-resistant E. faecium (VRE) strains. We screened the effect of these derivatives on biofilm formation and eradication. Derivatives 4e (for CRAB, VRE, and MRSA) and 4f (for all the strains) were the most potent ones and displayed activities as good as those of conventional antibiotics. We also identified 11 compounds able to decrease by more than 40% the production of pyocyanin, a major virulence factor of P. aeruginosa . We demonstrated that 4f treatment acts against bacterial infections in Galleria mellonella and significantly prolonged larvae survival (from 50% to 80%) after 24 h of CRAB, VRE, and MRSA infections. As shown by proteomic studies, 4f triggered distinct cellular responses depending on the bacterial species but essentially linked to cell envelope. Its interesting antibiofilm and antivirulence properties make it a promising a candidate for use in therapeutics.
- Published
- 2023
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35. Assessment of hypoxia and oxidative-related changes in a lung-derived brain metastasis model by [ 64 Cu][Cu(ATSM)] PET and proteomic studies.
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Fantin J, Toutain J, Pérès EA, Bernay B, Mehani SM, Helaine C, Bourgeois M, Brunaud C, Chazalviel L, Pontin J, Corroyer-Dulmont A, Valable S, Cherel M, and Bernaudin M
- Abstract
Background: Brain metastases (BM) are the most frequent malignant brain tumors. The aim of this study was to characterize the tumor microenvironment (TME) of BM and particularly hypoxia and redox state, known to play a role in tumor growth and treatment resistance with multimodal PET and MRI imaging, immunohistochemical and proteomic approaches in a human lung cancer (H2030-BrM3)-derived BM model in rats., Results: First, in vitro studies confirmed that H2030-BrM3 cells respond to hypoxia with increasing expression of HIF-1, HIF-2 and their target genes. Proteomic analyses revealed, among expression changes, proteins associated with metabolism, oxidative stress, metal response and hypoxia signaling in particular in cortical BM. [
64 Cu][Cu(ATSM)] PET revealed a significant uptake by cortical BM (p < 0.01), while no uptake is observed in striatal BM 23 days after tumor implantation. Pimonidazole, HIF-1α, HIF-2α, CA-IX as well as GFAP, CTR1 and DMT1 immunostainings are positive in both BM., Conclusion: Overall, [64 Cu][Cu(ATSM)] imaging and proteomic results showed the presence of hypoxia and protein expression changes linked to hypoxia and oxidative stress in BM, which are more pronounced in cortical BM compared to striatal BM. Moreover, it emphasized the interest of [64 Cu][Cu(ATSM)] PET to characterize TME of BM and depict inter-metastasis heterogeneity that could be useful to guide treatments., (© 2023. The Author(s).)- Published
- 2023
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36. Functional impact of oxygen-saturated zeolite nanoparticles on macrophages in the context of glioblastoma: an in vitro and in vivo study.
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Aurélie FE, Sarah K, Charly H, Clément A, Sajjad G, Julie C, Romaric S, Benoit B, Laurent C, Svetlana M, and Samuel V
- Subjects
- Humans, Proteomics, Hypoxia, Oxygen, Iron, Macrophages, Tumor Microenvironment, Glioblastoma, Zeolites, Nanoparticles
- Abstract
In the context of glioblastoma (GBM), hypoxia and inflammation are two main players of the tumor microenvironment. Hypoxia stimulates various features involves in tumor growth and also maintains a specific environment that favors protumor macrophages. Therefore, targeting hypoxia could potentially restore an anti-tumor M1 phenotype in macrophages. Besides, iron demonstrated its capacity to stimulate the polarization of macrophages towards an M1-like phenotype. In this paper we took advantages of microporous nanoparticles to co-deliver both oxygen and iron to bone marrow derived macrophages (BMDM) enabling the investigation of changes in polarization status and proteomic profiles. The nanoparticles were used in two in vivo models of glioblastoma, specifically, in both immunodeficient and immunocompetent settings. Our in vitro findings revealed that iron doped nanoparticles, saturated with oxygen were deemed safe for macrophages but did not demonstrate the capacity to change the M1 or M2 phenotypes. However, these nanoparticles induced some changes in proteomics pathways. The present study reports on in vivo experimentation that revealed the effects of nanoparticles on the hypoxic fraction, tumor volume, and macrophage phenotype in a GBM model. The findings indicated that the presence of nanoparticles led to a reduction in the hypoxic fraction in one of the GBM models, while no significant changes were observed in the tumor volume or macrophage phenotype. The present data showed that nanoparticles possess the capability of delivering both oxygen and iron to macrophages; though, they do not possess the ability to effectively repolarize M2 macrophages. Such strategies could be used in conjunction with other potent molecules to avoid M1 macrophages to inevitably differentiate to M2 macrophages., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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37. Comparative Proteome Analysis of Four Stages of Spermatogenesis in the Small-Spotted Catshark ( Scyliorhinus canicula ), Using High-Resolution NanoLC-ESI-MS/MS.
- Author
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Jeanne F, Bernay B, and Sourdaine P
- Subjects
- Male, Animals, Spermatogenesis, Testis, Spermatozoa, Spermatogonia metabolism, Proteome genetics, Proteome metabolism, Tandem Mass Spectrometry
- Abstract
Spermatogenesis is a highly specialized process of cell proliferation and differentiation leading to the production of spermatozoa from spermatogonial stem cells. Due to its testicular anatomy, Scyliorhinus canicula is an interesting model to explore stage-based changes in proteins during spermatogenesis. The proteomes of four testicular zones corresponding to the germinative niche and to spermatocysts (cysts) with spermatogonia (zone A), cysts with spermatocytes (zone B), cysts with young spermatids (zone C), and cysts with late spermatids (zone D) have been analyzed by nanoLC-ESI-MS/MS. Gene ontology and KEGG annotations were also performed. A total of 3346 multiple protein groups were identified. Zone-specific protein analyses highlighted RNA-processing, chromosome-related processes, cilium organization, and cilium activity in zones A, D, C, and D, respectively. Analyses of proteins with zone-dependent abundance revealed processes related to cellular stress, ubiquitin-dependent degradation by the proteasome, post-transcriptional regulation, and regulation of cellular homeostasis. Our results also suggest that the roles of some proteins, such as ceruloplasmin, optineurin, the pregnancy zone protein, PA28β or the Culling-RING ligase 5 complex, as well as some uncharacterized proteins, during spermatogenesis could be further explored. Finally, the study of this shark species allows one to integrate these data in an evolutionary context of the regulation of spermatogenesis. Mass spectrometry data are freely accessible via iProX-integrated Proteome resources (https://www.iprox.cn/) for reuse purposes.
- Published
- 2023
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38. Impacts of ocean acidification and warming on post-larval growth and metabolism in two populations of the great scallop (Pecten maximus).
- Author
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Harney E, Rastrick SPS, Artigaud S, Pisapia J, Bernay B, Miner P, Pichereau V, Strand Ø, Boudry P, and Charrier G
- Subjects
- Animals, Hydrogen-Ion Concentration, Seawater, Larva, Proteomics, Ocean Acidification, Temperature, Oxygen metabolism, Carbon Dioxide metabolism, Pecten metabolism, Pectinidae
- Abstract
Ocean acidification and warming are key stressors for many marine organisms. Some organisms display physiological acclimatization or plasticity, but this may vary across species ranges, especially if populations are adapted to local climatic conditions. Understanding how acclimatization potential varies among populations is therefore important in predicting species responses to climate change. We carried out a common garden experiment to investigate how different populations of the economically important great scallop (Pecten maximus) from France and Norway responded to variation in temperature and PCO2 concentration. After acclimation, post-larval scallops (spat) were reared for 31 days at one of two temperatures (13°C or 19°C) under either ambient or elevated PCO2 (pH 8.0 and pH 7.7). We combined measures of proteomic, metabolic and phenotypic traits to produce an integrative picture of how physiological plasticity varies between the populations. The proteome of French spat showed significant sensitivity to environmental variation, with 12 metabolic, structural and stress-response proteins responding to temperature and/or PCO2. Principal component analysis revealed seven energy metabolism proteins in French spat that were consistent with countering ROS stress under elevated temperature. Oxygen uptake in French spat did not change under elevated temperature but increased under elevated PCO2. In contrast, Norwegian spat reduced oxygen uptake under both elevated temperature and PCO2. Metabolic plasticity allows French scallops to maintain greater energy availability for growth compared with Norwegian spat. However, increased physiological plasticity and growth in French spat may come at a cost, as they showed reduced survival compared with Norwegian scallops under elevated temperature., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2023. Published by The Company of Biologists Ltd.)
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- 2023
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39. The first use of LC-MS/MS proteomic approach in the brown mussel Perna perna after bacterial challenge: Searching for key proteins on immune response.
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Silva Dos Santos F, Neves RAF, Bernay B, Krepsky N, Teixeira VL, and Artigaud S
- Subjects
- Humans, Animals, Escherichia coli, Proteomics, Ecosystem, Chromatography, Liquid, Tandem Mass Spectrometry, Bacteria, Immunity, Perna, Vibrio parahaemolyticus
- Abstract
The brown mussel Perna perna is a valuable fishing resource, primarily in tropical and subtropical coastal regions. Because of their filter-feeding habits, mussels are directly exposed to bacteria in the water column. Escherichia coli (EC) and Salmonella enterica (SE) inhabit human guts and reach the marine environment through anthropogenic sources, such as sewage. Vibrio parahaemolyticus (VP) is indigenous to coastal ecosystems but can be harmful to shellfish. In this study, we aimed to assess the protein profile of the hepatopancreas of P. perna mussel challenged by introduced - E. coli and S. enterica - and indigenous marine bacteria - V. parahaemolyticus. Bacterial-challenge groups were compared with non-injected (NC) and injected control (IC) - that consisted in mussels not challenged and mussels injected with sterile PBS-NaCl, respectively. Through LC-MS/MS proteomic analysis, 3805 proteins were found in the hepatopancreas of P. perna. From the total, 597 were significantly different among conditions. Mussels injected with VP presented 343 proteins downregulated compared with all the other conditions, suggesting that VP suppresses their immune response. Particularly, 31 altered proteins - upregulated or downregulated - for one or more challenge groups (EC, SE, and VP) compared with controls (NC and IC) are discussed in detail in the paper. For the three tested bacteria, significantly different proteins were found to perform critical roles in immune response at all levels, namely: recognition and signal transduction; transcription; RNA processing; translation and protein processing; secretion; and humoral effectors. This is the first shotgun proteomic study in P. perna mussel, therefore providing an overview of the protein profile of the mussel hepatopancreas, focused on the immune response against bacteria. Hence, it is possible to understand the immune-bacteria relationship at molecular levels better. This knowledge can support the development of strategies and tools to be applied to coastal marine resource management and contribute to the sustainability of coastal systems., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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40. Molecular approaches to uncover phage-lactic acid bacteria interactions in a model community simulating fermented beverages.
- Author
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Ledormand P, Desmasures N, Bernay B, Goux D, Rué O, Midoux C, Monnet C, and Dalmasso M
- Subjects
- Bacteria, Beverages microbiology, Fermentation, Fermented Beverages, Proteomics, Bacteriophages genetics, Lactobacillales genetics
- Abstract
Food microbial diversity and fluxes during the fermentation processes are well studied whereas phages-bacteria interactions are still poorly described in the literature. This is especially true in fermented beverages, and especially in cider, which is an alcoholic fermented apple beverage. The transcriptomic and proteomic responses of the lactic acid bacterium (LAB) Liquorilactobacillus mali UCMA 16447 to a lytic infection by phage UCMA 21115, both isolated from cider, were investigated, in order to get a better understanding of phages-bacteria interactions in such fermented beverage. During phage infection, 122 and 215 genes were differentially expressed in L. mali UCMA 16447 strain at T
15 and T60 respectively, when compared to the uninfected condition. The same trends were confirmed by the proteomic study, with a total of 28 differentially expressed proteins found at T60 . Overall, genes encoding cellular functions, such as carbohydrate metabolism, translation, and signal transduction, were downregulated, while genes involved in nucleotide metabolism and in the control of DNA integrity were upregulated in response to phage infection. This work also highlighted that phage infection repressed many genes involved in bacterial cell motility, and affected glycolysis., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2022
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41. Label-Free Direct Mass Spectrometry Analysis of the Bystander Effects Induced in Chondrocytes by Chondrosarcoma Cells Irradiated with X-rays and Carbon Ions.
- Author
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Gilbert A, Payet V, Bernay B, Chartier-Garcia E, Testard I, Candéias SM, and Chevalier F
- Subjects
- Bystander Effect radiation effects, Carbon, Chondrocytes, Culture Media, Conditioned pharmacology, Cytokines, Cytoplasmic Dyneins, DNA Helicases, Eukaryotic Initiation Factor-3, Human Umbilical Vein Endothelial Cells, Humans, Interleukin-12 pharmacology, Ions pharmacology, Mass Spectrometry, Nuclear Proteins, Proteome, Receptor, Platelet-Derived Growth Factor beta pharmacology, Transcription Factors, Tumor Microenvironment, X-Rays, Bone Neoplasms, Chondrosarcoma radiotherapy
- Abstract
Background: Radiation-induced bystander effects are induced changes in cells that were not themselves directly irradiated but were in the vicinity of a radiation path. Such effects, which occur in the microenvironment of an irradiated tumor, remain poorly understood and depend on the cell type and irradiation quality. This study aimed to evaluate bystander effects in non-irradiated chondrocytes that received conditioned medium from irradiated chondrosarcoma cells., Methods: SW1353 chondrosarcoma cells were irradiated with X-rays and carbon ions, each at 0.1 Gy and 2 Gy, and the conditioned media of the irradiated cells were transferred to T/C-28A2 chondrocytes and Human Umbilical Venous Endothelial Cells (HUVECs). The whole proteome of bystander chondrocytes was analyzed by label-free mass spectrometry, and a comparative study was performed by dose and irradiation quality. HUVECs were evaluated for inflammatory cytokine secretion., Results: The bystander response of chondrocytes to X-ray irradiation primarily affected the protein translation pathway (DHX36, EIF3B, EIF3D, EIF3M, EIF5, RPL6, RPLP0, RPS24, SYNCRIP), IL-12 (AIP, BOLA2, MIF, GAS6, MIF, PDGFRB) and the oxidative stress pathway (MGST3, PRDX2, PXDN, SOD2, TXN, TXNL1). Following carbon-ion irradiation, the G1/S pathway (PCBP4, PSMD12, PSME, XIAP) and mitotic G2 DNA damage checkpoint pathway (MRE11, TAOK1, UIMC1) were engaged. Changes in the regulation of chromosome separation (BCL7C, BUB3, CENPF, DYNC1LI1, SMARCA4, SMC4) were associated with only low-dose X-ray and carbon-ion irradiation. Modification of the protein translation pathway represented at least 30% of bystander effects and could play a role, possibly along with stress granules, in reduction in cellular metabolism to protect proteins. Stress granules were significantly enriched according to an interaction map., Conclusions: All these accessions corresponded to a window of the proteins modulated in response to the bystander effect. Our chondrosarcoma model clarified the nature of the bystander response of chondrocytes and may suggest several interesting new mechanisms that are specific to particular irradiation doses and qualities., Competing Interests: Given his role as Guest Editor, François Chevalier had no involvement in the peer-review of this article and has no access to information regarding its peer-review. Full responsibility for the editorial process for this article was delegated to Graham Pawelec. The authors declare no conflict of interest., (© 2022 The Author(s). Published by IMR Press.)
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- 2022
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42. Harsh intertidal environment enhances metabolism and immunity in oyster (Crassostrea gigas) spat.
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Corporeau C, Petton S, Vilaça R, Delisle L, Quéré C, Le Roy V, Dubreuil C, Lacas-Gervais S, Guitton Y, Artigaud S, Bernay B, Pichereau V, Huvet A, Petton B, Pernet F, Fleury E, Madec S, Brigaudeau C, Brenner C, and Mazure NM
- Subjects
- Animals, Host-Pathogen Interactions, Immunity, Innate, Proteomics, Crassostrea, Herpesviridae
- Abstract
The Pacific oyster Crassostrea gigas is established in the marine intertidal zone, experiencing rapid and highly dynamic environmental changes throughout the tidal cycle. Depending on the bathymetry, oysters face oxygen deprivation, lack of nutrients, and high changes in temperature during alternation of the cycles of emersion/immersion. Here we showed that intertidal oysters at a bathymetry level of 3 and 5 m delayed by ten days the onset of mortality associated with Pacific Oyster Mortality Syndrome (POMS) as compared to subtidal oysters. Intertidal oysters presented a lower growth but similar energetic reserves to subtidal oysters but induced proteomic changes indicative of a boost in metabolism, inflammation, and innate immunity that may have improved their resistance during infection with the Ostreid herpes virus. Our work highlights that intertidal harsh environmental conditions modify host-pathogen interaction and improve oyster health. This study opens new perspectives on oyster farming for mitigation strategies based on tidal height., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Corporeau Charlotte reports financial support was provided by Ifremer Brittany Centre. Corporeau charlotte reports a relationship with IFREMER that includes: employment., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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43. Structural and Functional Characterization of Orcokinin B-like Neuropeptides in the Cuttlefish ( Sepia officinalis ).
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Endress M, Zatylny-Gaudin C, Leprince J, Lefranc B, Corre E, Le Corguillé G, Bernay B, Leduc A, Rangama J, Mouret L, Lafont AG, Bondon A, and Henry J
- Subjects
- Amino Acid Sequence, Animals, Decapodiformes, Female, Neuropeptides, Sepia
- Abstract
The cuttlefish ( Sepia officinalis ) is a Cephalopod mollusk that lives in the English Channel and breeds in coastal spawning grounds in spring. A previous work showed that the control of egg-laying is monitored by different types of regulators, among which neuropeptides play a major role. They are involved in the integration of environmental cues, and participate in the transport of oocytes in the genital tract and in the secretion of capsular products. This study addresses a family of neuropeptides recently identified and suspected to be involved in the control of the reproduction processes. Detected by mass spectrometry and immunocytochemistry in the nerve endings of the accessory sex glands of the females and ovary, these neuropeptides are also identified in the hemolymph of egg-laying females demonstrating that they also have a hormone-like role. Released in the hemolymph by the sub-esophageal mass, a region that innervates the genital tract and the neurohemal area of the vena cava, in in vitro conditions these neuropeptides modulated oocyte transport and capsular secretion. Finally, in silico analyses indicated that these neuropeptides, initially called FLGamide, had extensive structural homology with orcokinin B, which motivated their name change.
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- 2022
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44. Integration of steam gasification and catalytic reforming of lignocellulosic biomass as a strategy to improve syngas quality and pollutants removal.
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Quiroga E, Cifuentes B, Moltó J, Ortuño N, Conesa J, Davó-Quiñonero A, and Cobo M
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- Biomass, Dibenzofurans, Gases chemistry, Lignin, Silicon Dioxide, Steam, Environmental Pollutants, Polychlorinated Dibenzodioxins
- Abstract
Residual biomass gasification is a promising route for the production of H
2 -rich syngas. However, the simultaneous formation of pollutants such as light hydrocarbons (HCs), benzene, toluene and xylenes (BTEX), polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) during gasification must be controlled. As a result, this study evaluated the effect of temperature and catalytic reforming over a Rh-Pt/CeO2 -SiO2 catalyst during steam gasification of sugarcane residual biomass on syngas composition and pollutant removal. The above was carried out in a horizontal moving reactor, an Amberlite XAD-2 polyaromatic resin was used to collect the contaminants and characterization of the catalyst was performed. In this study, a concentration of up to 37 mol% of H2 , a yield of 23.1 g H2 kg-1 biomass , and a H2 /CO ratio ≥2 were achieved when gasification and reforming were integrated. In addition, the catalyst characterization showed that Rh-Pt/CeO2 -SiO2 was not susceptible to sintering and favored the formation of hydroxyl groups that promoted CO oxidation, thereby increasing the H2 /CO ratio, as confirmed by in-situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). At 800 °C, where a high H2 yield was obtained, 209 g Nm-3 of light HCs and BTEX, 10.9 g Nm-3 of PAHs, and 32.5 ng WHO-TEQ Nm-3 of PCDD/Fs were formed after gasification. Interestingly, after catalytic reforming, 62% of light HCs and BTEX, 60% of PAHs, and 94% of PCDD/Fs were removed, leading to cleaner syngas with properties that allow it to be used in a wide range of energy applications., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2022
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45. Physiological and comparative proteomic analyzes reveal immune defense response of the king scallop Pecten maximus in presence of paralytic shellfish toxin (PST) from Alexandrium minutum.
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Even Y, Pousse E, Chapperon C, Artigaud S, Hégaret H, Bernay B, Pichereau V, Flye-Sainte-Marie J, and Jean F
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- Animals, Immunity, Marine Toxins toxicity, Proteomics, Seafood, Shellfish, Dinoflagellida physiology, Pecten metabolism, Pectinidae metabolism
- Abstract
The king scallop, Pecten maximus is a highly valuable seafood in Europe. Over the last few years, its culture has been threatened by toxic microalgae during harmful algal blooms, inducing public health concerns. Indeed, phycotoxins accumulated in bivalves can be harmful for human, especially paralytic shellfish toxins (PST) synthesized by the microalgae Alexandrium minutum. Deleterious effects of these toxic algae on bivalves have also been reported. However, its impact on bivalves such as king scallop is far from being completely understood. This study combined ecophysiological and proteomic analyzes to investigate the early response of juvenile king scallops to a short term exposure to PST producing A. minutum. Our data showed that all along the 2-days exposure to A. minutum, king scallops exhibited transient lower filtration and respiration rates and accumulated PST. Significant inter-individual variability of toxin accumulation potential was observed among individuals. Furthermore, we found that ingestion of toxic algae, correlated to toxin accumulation was driven by two factors: 1/ the time it takes king scallop to recover from filtration inhibition and starts to filtrate again, 2/ the filtration level to which king scallop starts again to filtrate after inhibition. Furthermore, at the end of the 2-day exposure to A. minutum, proteomic analyzes revealed an increase of the killer cell lectin-like receptor B1, involved in adaptative immune response. Proteins involved in detoxification and in metabolism were found in lower amount in A. minutum exposed king scallops. Proteomic data also showed differential accumulation in several structure proteins such as β-actin, paramyosin and filamin A, suggesting a remodeling of the mantle tissue when king scallops are subjected to an A. minutum exposure., (Copyright © 2022 Elsevier B.V. All rights reserved.)
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- 2022
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46. Identification of a New Set of Polypeptidic Sex Pheromones from Cuttlefish (Sepia officinalis).
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Gaudin-Zatylny C, Corre E, Zanuttini B, Endress M, Bernay B, Pontin J, Leduc A, and Henry J
- Subjects
- Animals, Decapodiformes, Female, Peptides, Proteomics, Sepia physiology, Sex Attractants
- Abstract
The common English Channel cuttlefish (Sepia officinalis) reproduces every year on very localized coastal spawning areas after a west-east horizontal migration of several tens of kilometers (80-200 km). The massive arrival of spawners on the coasts of west Cotentin and the Bay of Seine is suspected to be driven by the action of sex pheromones expressed and secreted by the genitals of sexually mature females. The present study aims to verify the existence of polypeptide pheromones, of a higher molecular weight than those described above. Their size could confer them a wider range of action than that of the previously identified peptide pheromones. The implementation of an experimental strategy combining transcriptomics and proteomics with functional tests and an in silico study led to the identification of a cocktail of pheromones with molecular weights ranging between 22 and 26 kDa. Proteomic analyses combined to functional tests revealed partial pheromone release in the environment, and their accumulation in the outer capsule of the egg, suggesting the eggs as pheromone diffusers, also able to induce stimulation by contact when the eggs are handled by females., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2022
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47. Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers.
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Brégeon M, Tomas D, Bernay B, Zatylny-Gaudin C, Georgeault S, Labas V, Réhault-Godbert S, and Guyot N
- Subjects
- Animals, Female, Ovum, Sperm-Ovum Interactions physiology, Tandem Mass Spectrometry, Chickens metabolism, Proteome metabolism
- Abstract
The avian egg perivitelline layer (PL) is a proteinaceous structure that encloses the egg yolk. It consists of the inner and the outer perivitelline layers (IPL and OPL, respectively) that are assumed to play distinct roles in bird reproduction. To gain insight into their respective function, we analyzed the proteome of IPL and OPL in chicken unfertilized eggs after mechanical separation, using a GeLC-MS/MS strategy. Of the 412 proteins identified, 173 proteins were uniquely recovered in IPL and 98 proteins in OPL, while 141 proteins were identified in both sublayers. Genes coding the most abundant proteins were shown to be expressed either in the liver/ovary (IPL formation) or in the oviduct (OPL formation), but rarely in both. The presence of oviduct-specific proteins (including LYZ, VMO1, AvBD11, PTN, OVAL and LOC10175704) in IPL strongly suggests that they participate in the physical association of IPL to OPL, whose tight attachment was further evidenced by analyses of IPL/OPL interfaces (by scanning electron microscopy). Functional annotation of identified proteins revealed functions associated with fertilization and early development for IPL, while OPL would rather participate in egg defense and embryogenesis. Collectively, our data highlight the complementary functions of IPL and OPL that are major determinants of bird reproductive success. SIGNIFICANCE: The present study unveils for the first time the individual proteomes of the two sublayers composing the chicken egg perivitelline layer (PL), which allowed to assign their respective putative biological roles in avian reproduction. The combination of proteomics with gene expression and ultrastructural analyses provides insightful data on the structure and biochemistry of the avian PL. The functional annotation of PL proteins highlights the multifaceted biological functions of this structure in reproduction including fertilization, embryonic development, and antimicrobial protection. This work will stimulate further research to validate predicted functions and to compare the physiology and the functional specificities of PL in egg-laying species., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
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- 2022
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48. Inactivation of the Response Regulator AgrA Has a Pleiotropic Effect on Biofilm Formation, Pathogenesis and Stress Response in Staphylococcus lugdunensis.
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Aubourg M, Pottier M, Léon A, Bernay B, Dhalluin A, Cacaci M, Torelli R, Ledormand P, Martini C, Sanguinetti M, Auzou M, Gravey F, and Giard JC
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- Animals, Bacterial Proteins genetics, Gene Expression Regulation, Bacterial, Humans, Hydrogen Peroxide pharmacology, Male, Mice, Mice, Inbred BALB C, Staphylococcus lugdunensis drug effects, Staphylococcus lugdunensis genetics, Virulence, Bacterial Proteins metabolism, Biofilms, Staphylococcal Infections microbiology, Staphylococcus lugdunensis pathogenicity, Staphylococcus lugdunensis physiology
- Abstract
Staphylococcus lugdunensis is a coagulase-negative Staphylococcus that emerges as an important opportunistic pathogen. However, little is known about the regulation underlying the transition from commensal to virulent state. Based on knowledge of S. aureus virulence, we suspected that the agr quorum sensing system may be an important determinant for the pathogenicity of S. lugdunensis . We investigated the functions of the transcriptional regulator AgrA using the agrA deletion mutant. AgrA played a role in cell pigmentation: Δ argA mutant colonies were white while the parental strains were slightly yellow. Compared with the wild-type strain, the Δ argA mutant was affected in its ability to form biofilm and was less able to survive in mice macrophages. Moreover, the growth of Δ agrA was significantly reduced by the addition of 10% NaCl or 0.4 mM H
2 O2 and its survival after 2 h in the presence of 1 mM H2 O2 was more than 10-fold reduced. To explore the mechanisms involved beyond these phenotypes, the Δ agrA proteome and transcriptome were characterized by mass spectrometry and RNA-Seq. We found that AgrA controlled several virulence factors as well as stress-response factors, which are well correlated with the reduced resistance of the Δ agrA mutant to osmotic and oxidative stresses. These results were not the consequence of the deregulation of RNAIII of the agr system, since no phenotype or alteration of the proteomic profile has been observed for the ΔRNAIII mutant. Altogether, our results highlighted that the AgrA regulator of S. lugdunensis played a key role in its ability to become pathogenic. IMPORTANCE Although belonging to the natural human skin flora, Staphylococcus lugdunensis is recognized as a particularly aggressive and destructive pathogen. This study aimed to characterize the role of the response regulator AgrA, which is a component of the quorum-sensing agr system and known to be a major element in the regulation of pathogenicity and biofilm formation in Staphylococcus aureus. In the present study, we showed that, contrary to S. aureus, the agrA deletion mutant produced less biofilm. Inactivation of agrA conferred a white colony phenotype and impacted S. lugdunensis in its ability to survive in mice macrophages and to cope with osmotic and oxidative stresses. By global proteomic and transcriptomic approaches, we identified the AgrA regulon, bringing molecular bases underlying the observed phenotypes. Together, our data showed the importance of AgrA in the opportunistic pathogenic behavior of S. lugdunensis allowing it to be considered as an interesting therapeutic target.- Published
- 2022
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49. Vibration Energy Harvesting by Means of Piezoelectric Patches: Application to Aircrafts.
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Tommasino D, Moro F, Bernay B, De Lumley Woodyear T, de Pablo Corona E, and Doria A
- Abstract
Vibration energy harvesters in industrial applications usually take the form of cantilever oscillators covered by a layer of piezoelectric material and exploit the resonance phenomenon to improve the generated power. In many aeronautical applications, the installation of cantilever harvesters is not possible owing to the lack of room and/or safety and durability requirements. In these cases, strain piezoelectric harvesters can be adopted, which directly exploit the strain of a vibrating aeronautic component. In this research, a mathematical model of a vibrating slat is developed with the modal superposition approach and is coupled with the model of a piezo-electric patch directly bonded to the slat. The coupled model makes it possible to calculate the power generated by the strain harvester in the presence of the broad-band excitation typical of the aeronautic environment. The optimal position of the piezoelectric patch along the slat length is discussed in relation with the modes of vibration of the slat. Finally, the performance of the strain piezoelectric harvester is compared with the one of a cantilever harvester tuned to the frequency of the most excited slat mode.
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- 2022
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50. The benefit of combining curcumin, bromelain and harpagophytum to reduce inflammation in osteoarthritic synovial cells.
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Brochard S, Pontin J, Bernay B, Boumediene K, Conrozier T, and Baugé C
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- Cell Line, Drug Therapy, Combination, France, Harpagophytum, Humans, Proteomics, Spain, Anti-Inflammatory Agents pharmacology, Bromelains pharmacology, Curcumin pharmacology, Osteoarthritis drug therapy, Plant Extracts pharmacology, Synovial Membrane drug effects
- Abstract
Background: Osteoarthritis (OA) is the most common form of arthritis, affecting millions of people worldwide and characterised by joint pain and inflammation. It is a complex disease involving inflammatory factors and affecting the whole joint, including the synovial membrane. Since drug combination is widely used to treat chronic inflammatory diseases, a similar strategy of designing plant-derived natural products to reduce inflammation in OA joints may be of interest. In this study, we characterised the response of OA synovial cells to lipopolysaccharide (LPS) and investigated the biological action of the combination of curcumin, bromelain and harpagophytum in this original in vitro model of osteoarthritis., Methods: Firstly, human synovial cells from OA patients were stimulated with LPS and proteomic analysis was performed. Bioinformatics analyses were performed using Cytoscape App and SkeletalVis databases. Additionally, cells were treated with curcumin, bromelain and harpagophytum alone or with the three vegetal compounds together. The gene expression involved in inflammation, pain or catabolism was determined by RT-PCR. The release of the encoded proteins by these genes and of prostaglandin E2 (PGE2) were also assayed by ELISA., Results: Proteomic analysis demonstrated that LPS induces the expression of numerous proteins involved in the OA process in human OA synovial cells. In particular, it stimulates inflammation through the production of pro-inflammatory cytokines (Interleukin-6, IL-6), catabolism through an increase of metalloproteases (MMP-1, MMP-3, MMP-13), and the production of pain-mediating neurotrophins (Nerve Growth Factor, NGF). These increases were observed in terms of mRNA levels and protein release. LPS also increases the amount of PGE2, another inflammation and pain mediator. At the doses tested, vegetal extracts had little effect: only curcumin slightly counteracted the effects of LPS on NGF and MMP-13 mRNA, and PGE2, IL-6 and MMP-13 release. In contrast, the combination of curcumin with bromelain and harpagophytum reversed lots of effects of LPS in human OA synovial cells. It significantly reduced the gene expression and/or the release of proteins involved in catabolism (MMP-3 and -13), inflammation (IL-6) and pain (PGE2 and NGF)., Conclusion: We have shown that the stimulation of human OA synovial cells with LPS can induce protein changes similar to inflamed OA synovial tissues. In addition, using this model, we demonstrated that the combination of three vegetal compounds, namely curcumin, bromelain and harpagophytum, have anti-inflammatory and anti-catabolic effects in synovial cells and may thus reduce OA progression and related pain., (© 2021. The Author(s).)
- Published
- 2021
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