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6. High-Statistics Sub-Barrier Coulomb Excitation of 106,108,110Sn

Author

Park, J., primary, Knyazev, A., additional, Rickert, E., additional, Golubev, P., additional, Cederkäll, J., additional, Andreyev, A. N., additional, de Angelis, G., additional, Arnswald, K., additional, Barber, L., additional, Berger, C., additional, Berner, C., additional, Berry, T., additional, Borge, M. J. G., additional, Boukhari, A., additional, Cox, D., additional, Cubiss, J., additional, Cullen, D. M., additional, Ovejas, J. Díaz, additional, Fahlander, C., additional, Gaffney, L. P., additional, Gawlik, A., additional, Gernhäuser, R., additional, Görgen, A., additional, Habermann, T., additional, Henrich, C., additional, Illana, A., additional, Iwanicki, J., additional, Johansen, T. W., additional, Konki, J., additional, Kröll, T., additional, Nara Singh, B. S., additional, Rainovski, G., additional, Raison, C., additional, Reiter, P., additional, Rosiak, D., additional, Saha, S., additional, Saxena, M., additional, Schilling, M., additional, Seidlitz, M., additional, Snäll, J., additional, Stahl, C., additional, Stryjczyk, M., additional, Tengblad, O., additional, Tveten, G. M., additional, Valiente-Dobón, J. J., additional, Van Duppen, P., additional, Viñals, S., additional, Warr, N., additional, Welker, A., additional, Werner, L., additional, De Witte, H., additional, and Zidarova, R., additional

Published
2020
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7. Spectroscopy of particle-phonon coupled states in 133Sb by the cluster transfer reaction 132Sn on 7Li: an advanced test of nuclear interactions

Author

Leoni, Silvia, Benzoni, Giovanna, Bottoni, Simone, Bracco, Angela, Camera, Franco, Crespi, Fabio, Iskra, Lukasz, Porzio, Carlotta, Ziliani, Sara, Fornal, Bogdan, Bednarczyk, Piotr, Cieplicka-Orynczak, Natalia, Maj, Adam, Rusek, Krzysztof, Bazzacco, Dino, Mengoni, Daniele, Recchia, Francesco, Valiente-Dobon, Javier, Gramegna, Fabiana, Marchi, Tommaso, Raabe, Riccardo, Van Duppen, Piet, Sferrazza, Michele, Georgiev, Georgi, Reiter, Peter, Blazhev, A, Seidlitz, M, Warr, N, Kroell, Thorsten, Hartig, AL, Henrich, C, Homm, I, Surder, C, Rhee, HB, Gernhauser, Roman, Berner, C, Mucher, Dennis, Wimmer, Kathrin, Carpenter, Mike, Zhu, Shaofei, Janssens, Robert, Kowalska, Magna, Marginean, Nicu, Marginean, Raluca, Lica, Razvan, Clisu, Cristina, and Ur, Calin

Subjects
Detectors and Experimental Techniques
Published
2019

8. How to Take 30 Years Off the Life of an Earth-Orbiter Network

Author

Berner, C. D, Perkins, R. C, and Baker, N

Subjects
Communications And Radar
Abstract

In the mid 1960's the NASA/JPL Deep Space Network installed a global 26-meter antenna network to support a large group of Low Earth Orbiters and Highly Elliptical Orbiters. Although this network was equipped with then state-of-the-art equipment, operations were labor- intensive. A study is discussed which takes a close look at re- engineering the 26-meter antenna network from all aspects.

Published
1995

10. COULOMB EXCITATION OF Xe-142

Author

Henrich, C., Kroell, Th., Arnswald, K., Berger, C., Berner, C., Berry, T., Bildstein, V., Cederkall, J., Cox, D., de Angelis, G., de Witte, H., Martinez, G. Fernandez, Gaffney, L., Georgiev, G., Ilieva, S., Sison, A. Illana, Lozeva, R., Matejska-Minda, M., Napiorkowski, P. J., Ojala, J., Pakarinen, J., Rainovski, G., Ramdhane, M., Reiter, P., Rhee, H-B, Rosiak, D., Seidlitz, M., Siebeck, B., Simpson, G., Snall, J., Vaquero Soto, V., Thuerauf, M., von Schmid, M., Warr, N., Werner, L., Zielinska, M., Henrich, C., Kroell, Th., Arnswald, K., Berger, C., Berner, C., Berry, T., Bildstein, V., Cederkall, J., Cox, D., de Angelis, G., de Witte, H., Martinez, G. Fernandez, Gaffney, L., Georgiev, G., Ilieva, S., Sison, A. Illana, Lozeva, R., Matejska-Minda, M., Napiorkowski, P. J., Ojala, J., Pakarinen, J., Rainovski, G., Ramdhane, M., Reiter, P., Rhee, H-B, Rosiak, D., Seidlitz, M., Siebeck, B., Simpson, G., Snall, J., Vaquero Soto, V., Thuerauf, M., von Schmid, M., Warr, N., Werner, L., and Zielinska, M.

Abstract

The even-even nucleus Xe-142 lies north-east of the doubly magic Sn-132 on the neutron-rich side of the nuclear chart. In order to gain further information on the octupole collectivity and the evolution of quadrupole collectivity in this region, a safe Coulomb excitation experiment was carried out at the new HIE-ISOLDE facility (CERN) at the end of 2016. As the gamma-ray detector the Miniball spectrometer was used. Beam and target nuclei were detected using C-REX, i.e. an array of segmented Si detectors, covering forward as well as backward angles in the laboratory frame.

Published
2018

11. Coulomb Excitation of (142) Xe

Author

European Commission, Federal Ministry of Education and Research (Germany), SCOAP, Henrich, C., Kröll, Th., Arnswald, K., Berger, C., Berner, C., Berry, T., Bildstein, V., Cederkäll, J., Cox, D., De Angelis, G., De Witte, H., Fernández Martínez, G., Gaffney, L., Georgiev, G., Ilieva, S., Illana Sisón, A., Lozeva, R., Matejska-Minda, M., Napiorkowski, P. J., Ojala, J. Pakarinen, J., Rainovski, G., Ramdhane, M., Reiter, P., Rhee, H. -B., Rosiak, D., Seidlitz, M., Siebeck, B., Simpson, G., Snäll, J., Vaquero, Victor, Thürauf, M., von Schmid, M., Warr, N., Werner, L., Zielińska, M., European Commission, Federal Ministry of Education and Research (Germany), SCOAP, Henrich, C., Kröll, Th., Arnswald, K., Berger, C., Berner, C., Berry, T., Bildstein, V., Cederkäll, J., Cox, D., De Angelis, G., De Witte, H., Fernández Martínez, G., Gaffney, L., Georgiev, G., Ilieva, S., Illana Sisón, A., Lozeva, R., Matejska-Minda, M., Napiorkowski, P. J., Ojala, J. Pakarinen, J., Rainovski, G., Ramdhane, M., Reiter, P., Rhee, H. -B., Rosiak, D., Seidlitz, M., Siebeck, B., Simpson, G., Snäll, J., Vaquero, Victor, Thürauf, M., von Schmid, M., Warr, N., Werner, L., and Zielińska, M.

Abstract

The even–even nucleus 142Xe lies north-east of the doubly magic 132Sn on the neutron-rich side of the nuclear chart. In order to gain further information on the octupole collectivity and the evolution of quadrupole collectivity in this region, a “safe” Coulomb excitation experiment was carried out at the new HIE-ISOLDE facility (CERN) at the end of 2016. As the gamma-ray detector the Miniball spectrometer was used. Beam and target nuclei were detected using C-REX, i.e. an array of segmented Si detectors, covering forward as well as backward angles in the laboratory frame.

Published
2018

12. Coulomb Excitation of $^{142}$Xe

Author

Henrich, C., primary, Kröll, Th., additional, Arnswald, K., additional, Berger, C., additional, Berner, C., additional, Berry, T., additional, Bildstein, V., additional, Cederkäll, J., additional, Cox, D., additional, de Angelis, G., additional, De Witte, H., additional, Fernández Martínez, G., additional, Gaffney, L., additional, Georgiev, G., additional, Ilieva, S., additional, Illana Sisón, A., additional, Lozeva, R., additional, Matejska-Minda, M., additional, Napiorkowski, P.J., additional, Ojala, J., additional, Pakarinen, J., additional, Rainovski, G., additional, Ramdhane, M., additional, Reiter, P., additional, Rhee, H.-B., additional, Rosiak, D., additional, Seidlitz, M., additional, Siebeck, B., additional, Simpson, G., additional, Snäll, J., additional, Vaquero Soto, V., additional, Thürauf, M., additional, von Schmid, M., additional, Warr, N., additional, Werner, L., additional, and Zielińska, M., additional

Published
2018
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15. Aerodynamic coefficients identification procedure of a finned projectile using magnetometers and videos free flight data

Author

Albisser, M., Berner, C., Dobre, S., Thomassin, M., Hugues Garnier, Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut franco-allemand de recherches de Saint-Louis (ISL), DGA-Matériaux et nanosciences d'Alsace (FMNGE), and Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Identification, [INFO.INFO-AU]Computer Science [cs]/Automatic Control Engineering, High speed videos, Free flight, Aerodynamic coefficients estimation, Magnetometers
Abstract

International audience; This paper presents a new procedure for the identification of the aerodynamic coefficients of vehicles in flight. This approach is based on system identification techniques and by use of free flight data obtained by means of measurement techniques like magnetometers and high speed videos. Several steps are required, like the polynomial description of the aerodynamic coefficients as a function of Mach number and angle of attack, identifiability analysis followed by the estimation of the model parameters. Based on experimental free flight measurements of the Basic Finner reference projectile, these estimation results are then compared to existing ones in literature. Moreover, the estimation precision is improved by taking into account the parameter estimation problem through a multiple fit strategy.

Published
2014

17. Search for Top Squark and Higgsino Production Using Diphoton Higgs Boson Decays

Author

Chatrchyan, S. Khachatryan, V. Sirunyan, A. M. Tumasyan, A. and Adam, W. Bergauer, T. Dragicevic, M. Ero, J. Fabjan, C. Friedl, M. Fruehwirth, R. Ghete, V. M. Hartl, C. and Hoermann, N. Hrubec, J. Jeitler, M. Kiesenhofer, W. and Knuenz, V. Krammer, M. Kraetschmer, I. Liko, D. Mikulec, I. Rabady, D. Rahbaran, B. Rohringer, H. Schoefbeck, R. and Strauss, J. Taurok, A. Treberer-Treberspurg, W. and Waltenberger, W. Wulz, C. -E. Mossolov, V. Shumeiko, N. and Gonzalez, J. Suarez Alderweireldt, S. Bansal, M. Bansal, S. and Cornelis, T. De Wolf, E. A. Janssen, X. Knutsson, A. and Luyckx, S. Mucibello, L. Ochesanu, S. Roland, B. Rougny, R. Van Haevermaet, H. Van Mechelen, P. Van Remortel, N. and Van Spilbeeck, A. Blekman, F. Blyweert, S. D'Hondt, J. and Heracleous, N. Kalogeropoulos, A. Keaveney, J. Kim, T. J. and Lowette, S. Maes, M. Olbrechts, A. Strom, D. and Tavernier, S. Van Doninck, W. Van Mulders, P. Van Onsem, G. P. Villella, I. Caillol, C. Clerbaux, B. De Lentdecker, G. Favart, L. Gay, A. P. R. Leonard, A. Marage, P. E. and Mohammadi, A. Pernie, L. Reis, T. Seva, T. Thomas, L. Vander Velde, C. Vanlaer, P. Wang, J. Adler, V. and Beernaert, K. Benucci, L. Cimmino, A. Costantini, S. and Dildick, S. Garcia, G. Klein, B. Lellouch, J. Mccartin, J. Rios, A. A. Ocampo Ryckbosch, D. Diblen, S. Salva and Sigamani, M. Strobbe, N. Thyssen, F. Tytgat, M. Walsh, S. Yazgan, E. Zaganidis, N. Basegmez, S. Beluffi, C. and Bruno, G. Castello, R. Caudron, A. Ceard, L. Da Silveira, G. G. Delaere, C. Du Pree, T. Favart, D. and Forthomme, L. Giammanco, A. Hollar, J. Jez, P. Komm, M. and Lemaitre, V. Liao, J. Militaru, O. Nuttens, C. and Pagano, D. Pin, A. Piotrzkowski, K. Popov, A. and Quertenmont, L. Selvaggi, M. Marono, M. Vidal Garcia, J. M. Vizan Beliy, N. Caebergs, T. Daubie, E. Hammad, G. H. and Alves, G. A. Correa Martins Junior, M. Martins, T. Pol, M. E. Souza, M. H. G. Alda Junior, W. L. Carvalho, W. and Chinellato, J. Custodio, A. Da Costa, E. M. De Jesus Damiao, D. De Oliveira Martins, C. Fonseca De Souza, S. Malbouisson, H. Malek, M. Matos Figueiredo, D. Mundim, L. Nogima, H. and Prado Da Silva, W. L. Santaolalla, J. Santoro, A. and Sznajder, A. Tonelli Manganote, E. J. Vilela Pereira, A. and Bernardes, C. A. Dias, F. A. Fernandez Perez Tomei, T. R. and Gregores, E. M. Lagana, C. Mercadante, P. G. Novaes, S. F. and Padula, S. S. Genchev, V. Iaydjiev, P. Marinov, A. and Piperov, S. Rodozov, M. Sultanov, G. Vutova, M. and Dimitrov, A. Glushkov, I. Hadjiiska, R. Kozhuharov, V. and Litov, L. Pavlov, B. Petkov, P. Bian, J. G. Chen, G. M. and Chen, H. S. Chen, M. Du, R. Jiang, C. H. Liang, D. and Liang, S. Meng, X. Plestina, R. Tao, J. Wang, X. and Wang, Z. Asawatangtrakuldee, C. Ban, Y. Guo, Y. Li, Q. and Li, W. Liu, S. Mao, Y. Qian, S. J. Wang, D. and Zhang, L. Zou, W. Avila, C. Carrillo Montoya, C. A. and Chaparro Sierra, L. F. Florez, C. Gomez, J. P. Gomez Moreno, B. Sanabria, J. C. Godinovic, N. Lelas, D. Polic, D. and Puljak, I. Antunovic, Z. Kovac, M. Brigljevic, V. and Kadija, K. Luetic, J. Mekterovic, D. Morovic, S. and Tikvica, L. Attikis, A. Mavromanolakis, G. Mousa, J. and Nicolaou, C. Ptochos, F. Razis, P. A. Finger, M. Finger, Jr., M. Abdelalim, A. A. Assran, Y. Elgammal, S. Kamel, A. Ellithi Mahmoud, M. A. Radi, A. Kadastik, M. Muentel, M. Muruma, M. Raidal, M. Rebane, L. Tiko, A. Eerola, P. Fedi, G. Voutilainen, M. Haerkoenen, J. Karimaeki, V. and Kinnunen, R. Kortelainen, M. J. Lampen, T. and Lassila-Perini, K. Lehti, S. Linden, T. Luukka, P. and Maeenoaeae, T. Peltola, T. Tuominen, E. Tuominiemi, J. and Tuovinen, E. Wendland, L. Tuuva, T. Besancon, M. and Couderc, F. Dejardin, M. Denegri, D. Fabbro, B. Faure, J. L. Ferri, F. Ganjour, S. Givernaud, A. Gras, P. and de Monchenault, G. Hamel Jarry, P. Locci, E. Malcles, J. and Nayak, A. Rander, J. Rosowsky, A. Titov, M. Baffioni, S. and Beaudette, F. Busson, P. Charlot, C. Daci, N. Dahms, T. Dalchenko, M. Dobrzynski, L. Florent, A. De Cassagnac, R. Granier Mine, P. Mironov, C. Naranjo, I. N. and Nguyen, M. Ochando, C. Paganini, P. Sabes, D. and Salerno, R. Sirois, Y. Veelken, C. Yilmaz, Y. Zabi, A. and Agram, J. -L. Andrea, J. Bloch, D. Brom, J. -M. and Chabert, E. C. Collard, C. Conte, E. Drouhin, F. and Fontaine, J. -C. Gele, D. Goerlach, U. Goetzmann, C. and Juillot, P. Le Bihan, A. -C. Van Hove, P. Gadrat, S. and Beauceron, S. Beaupere, N. Boudoul, G. Brochet, S. and Chasserat, J. Chierici, R. Contardo, D. Depasse, P. El Mamouni, H. Fan, J. Fay, J. Gascon, S. Gouzevitch, M. and Ille, B. Kurca, T. Lethuillier, M. Mirabito, L. and Perries, S. Alvarez, J. D. Ruiz Sgandurra, L. Sordini, V. and Donckt, M. Vander Verdier, P. Viret, S. Xiao, H. and Tsamalaidze, Z. Autermann, C. Beranek, S. Bontenackels, M. and Calpas, B. Edelhoff, M. Feld, L. Hindrichs, O. and Klein, K. Ostapchuk, A. Perieanu, A. Raupach, F. Sammet, J. Schael, S. Sprenger, D. Weber, H. Wittmer, B. and Zhukov, V. Ata, M. Caudron, J. Dietz-Laursonn, E. and Duchardt, D. Erdmann, M. Fischer, R. Gueth, A. Hebbeker, T. Heidemann, C. Hoepfner, K. Klingebiel, D. Knutzen, S. and Kreuzer, P. Merschmeyer, M. Thueer, S. Olschewski, M. and Padeken, K. Papacz, P. Reithler, H. Schmitz, S. A. and Sonnenschein, L. Teyssier, D. Thuer, S. Weber, M. and Cherepanov, V. Erdogan, Y. Fluegge, G. Geenen, H. and Geisler, M. Ahmad, W. Haj Hoehle, F. Kargoll, B. Kress, T. Kuessel, Y. Lingemann, J. Nowack, A. Nugent, I. M. and Perchalla, L. Pooth, O. Stahl, A. Asin, I. Bartosik, N. Behr, J. Behrenhoff, W. Behrens, U. Bell, A. J. and Bergholz, M. Bethani, A. Borras, K. Burgmeier, A. Cakir, A. Calligaris, L. Campbell, A. Choudhury, S. Costanza, F. Pardos, C. Diez Dooling, S. Dorland, T. Eckerlin, G. and Eckstein, D. Eichhorn, T. Flucke, G. Geiser, A. and Grebenyuk, A. Gunnellini, P. Habib, S. Hauk, J. Hellwig, G. Hempel, M. Horton, D. Jung, H. Kasemann, M. and Katsas, P. Kieseler, J. Kleinwort, C. Kraemer, M. and Kruecker, D. Lange, W. Leonard, J. Lipka, K. Lohmann, W. and Lutz, B. Mankel, R. Marfin, I. Melzer-Pellmann, I. -A. and Meyer, A. B. Mnich, J. Mussgiller, A. Naumann-Emme, S. and Novgorodova, O. Nowak, F. Perrey, H. Petrukhin, A. and Pitzl, D. Placakyte, R. Raspereza, A. Cipriano, P. M. Ribeiro Riedl, C. Ron, E. Sahin, M. O. Salfeld-Nebgen, J. Saxena, P. Schmidt, R. Schoerner-Sadenius, T. and Schroeder, M. Stein, M. Trevino, A. D. R. Vargas Walsh, R. and Wissing, C. Martin, M. Aldaya Blobel, V. Enderle, H. and Erfle, J. Garutti, E. Goebel, K. Goerner, M. Gosselink, M. Haller, J. Hoeing, R. S. Kirschenmann, H. Klanner, R. and Kogler, R. Lange, J. Lapsien, T. Marchesini, I. Ott, J. Peiffer, T. Pietsch, N. Rathjens, D. Sander, C. and Schettler, H. Schleper, P. Schlieckau, E. Schmidt, A. and Seidel, M. Sibille, J. Sola, V. Stadie, H. Steinbrueck, G. Troendle, D. Usai, E. Vanelderen, L. Barth, C. and Baus, C. Berger, J. Boeser, C. Butz, E. Chwalek, T. and De Boer, W. Descroix, A. Dierlamm, A. Feindt, M. and Guthoff, M. Hartmann, F. Hauth, T. Held, H. Hoffmann, K. H. Husemann, U. Katkov, I. Kornmayer, A. Kuznetsova, E. and Pardo, P. Lobelle Martschei, D. Mozer, M. U. Mueller, T. and Niegel, M. Nuerenberg, A. Oberst, O. Quast, G. and Rabbertz, K. Ratnikov, F. Roecker, S. Schilling, F. -P. and Schott, G. Simonis, H. J. Stober, F. M. Ulrich, R. and Wagner-Kuhr, J. Wayand, S. Weiler, T. Wolf, R. Zeise, M. and Anagnostou, G. Daskalakis, G. Geralis, T. Kesisoglou, S. and Kyriakis, A. Loukas, D. Markou, A. Markou, C. and Ntomari, E. Psallidas, A. Topsis-Giotis, I. Gouskos, L. and Panagiotou, A. Saoulidou, N. Stiliaris, E. Aslanoglou, X. and Evangelou, I. Flouris, G. Foudas, C. Jones, J. and Kokkas, P. Manthos, N. Papadopoulos, I. Paradas, E. and Bencze, G. Hajdu, C. Hidas, P. Horvath, D. Sikler, F. and Veszpremi, V. Vesztergombi, G. Zsigmond, A. J. Beni, N. and Czellar, S. Molnar, J. Palinkas, J. Szillasi, Z. and Karancsi, J. Raics, P. Trocsanyi, Z. L. Ujvari, B. and Swain, S. K. Beri, S. B. Bhatnagar, V. Dhingra, N. and Gupta, R. Kaur, M. Mehta, M. Z. Mittal, M. Nishu, N. and Sharma, A. Singh, J. B. Kumar, A. Kumar, A. Ahuja, S. and Bhardwaj, A. Choudhary, B. C. Kumar, A. Malhotra, S. and Naimuddin, M. Ranjan, K. Sharma, V. Shivpuri, R. K. and Banerjee, S. Bhattacharya, S. Chatterjee, K. Dutta, S. and Gomber, B. Jain, S. Jain, S. Khurana, R. Modak, A. and Mukherjee, S. Roy, D. Sarkar, S. Sharan, M. Singh, A. P. and Abdulsalam, A. Dutta, D. Kailas, S. Kumar, V. and Mohanty, A. K. Pant, L. M. Shukla, P. Topkar, A. Aziz, T. Chatterjee, R. M. Ganguly, S. Ghosh, S. Guchait, M. and Gurtu, A. Kole, G. Kumar, S. Maity, M. Majumder, G. and Mazumdar, K. Mohanty, G. B. Parida, B. Sudhakar, K. and Wickramage, N. Banerjee, S. Dugad, S. Arfaei, H. and Bakhshiansohi, H. Behnamian, H. Etesami, S. M. Fahim, A. and Jafari, A. Khakzad, M. Najafabadi, M. Mohammadi Naseri, M. and Mehdiabadi, S. Paktinat Safarzadeh, B. Zeinali, M. and Grunewald, M. Abbrescia, M. Barbone, L. Calabria, C. and Chhibra, S. S. Colaleo, A. Creanza, D. De Filippis, N. and De Palma, M. Fiore, L. Iaselli, G. Maggi, G. Maggi, M. and Marangelli, B. My, S. Nuzzo, S. Pacifico, N. and Pompili, A. Pugliese, G. Radogna, R. Selvaggi, G. and Silvestris, L. Singh, G. Venditti, R. Verwilligen, P. and Zito, G. Abbiendi, G. Benvenuti, A. C. Bonacorsi, D. and Braibant-Giacomelli, S. Brigliadori, L. Campanini, R. and Capiluppi, P. Castro, A. Cavallo, F. R. Codispoti, G. and Cuffiani, M. Dallavalle, G. M. Fabbri, F. Fanfani, A. and Fasanella, D. Giacomelli, P. Grandi, C. Guiducci, L. and Marcellini, S. Masetti, G. Meneghelli, M. Montanari, A. and Navarria, F. L. Odorici, F. Perrotta, A. Primavera, F. and Rossi, A. M. Rovelli, T. Siroli, G. P. Tosi, N. and Travaglini, R. Albergo, S. Cappello, G. Chiorboli, M. and Costa, S. Giordano, F. Potenza, R. Tricomi, A. Tuve, C. and Barbagli, G. Ciulli, V. Civinini, C. D'Alessandro, R. and Focardi, E. Gallo, E. Gonzi, S. Gori, V. Lenzi, P. and Meschini, M. Paoletti, S. Sguazzoni, G. Tropiano, A. and Benussi, L. Bianco, S. Fabbri, F. Piccolo, D. and Fabbricatore, P. Ferretti, R. Ferro, F. Lo Vetere, M. and Musenich, R. Robutti, E. Tosi, S. Benaglia, A. Dinardo, M. E. Fiorendi, S. Gennai, S. Gerosa, R. Ghezzi, A. and Govoni, P. Lucchini, M. T. Malvezzi, S. Manzoni, R. A. and Martelli, A. Marzocchi, B. Menasce, D. Moroni, L. and Paganoni, M. Pedrini, D. Ragazzi, S. Redaelli, N. De Fatis, T. Tabarelli Buontempo, S. Cavallo, N. Fabozzi, F. and Iorio, A. O. M. Lista, L. Meola, S. Merola, M. and Paolucci, P. Azzi, P. Bacchetta, N. Bisello, D. Branca, A. Carlin, R. Checchia, P. Dorigo, T. Galanti, M. and Gasparini, F. Gasparini, U. Giubilato, P. Gozzelino, A. and Kanishchev, K. Lacaprara, S. Lazzizzera, I. Margoni, M. and Meneguzzo, A. T. Passaseo, M. Pazzini, J. Pegoraro, M. and Pozzobon, N. Ronchese, P. Simonetto, F. Torassa, E. and Tosi, M. Triossi, A. Ventura, S. Zucchetta, A. Zumerle, G. Gabusi, M. Ratti, S. P. Riccardi, C. Vitulo, P. and Biasini, M. Bilei, G. M. Fano, L. 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Markowitz, P. Martinez, G. Rodriguez, J. L. and Adams, T. Askew, A. Bochenek, J. Chen, J. Diamond, B. and Haas, J. Hagopian, S. Hagopian, V. Johnson, K. F. and Prosper, H. Veeraraghavan, V. Weinberg, M. Baarmand, M. M. and Dorney, B. Hohlmann, M. Kalakhety, H. Yumiceva, F. and Adams, M. R. Apanasevich, L. Bazterra, V. E. Betts, R. R. and Bucinskaite, I. Cavanaugh, R. Evdokimov, O. Gauthier, L. and Gerber, C. E. Hofman, D. J. Khalatyan, S. Kurt, P. and Moon, D. H. O'Brien, C. Silkworth, C. Turner, P. and Varelas, N. Akgun, U. Albayrak, E. A. Bilki, B. Clarida, W. Dilsiz, K. Duru, F. Haytmyradov, M. Merlo, J. -P. and Mermerkaya, H. Mestvirishvili, A. Moeller, A. Nachtman, J. and Ogul, H. Onel, Y. Ozok, F. Sen, S. Tan, P. and Tiras, E. Wetzel, J. Yetkin, T. Yi, K. Barnett, B. A. and Blumenfeld, B. Bolognesi, S. Fehling, D. Gritsan, A. V. and Maksimovic, P. Martin, C. Swartz, M. Baringer, P. and Bean, A. Benelli, G. Kenny, R. P. Murray, M. Noonan, D. and Sanders, S. Sekaric, J. Stringer, R. Wang, Q. Wood, J. S. Barfuss, A. F. Chakaberia, I. Ivanov, A. Khalil, S. Makouski, M. Maravin, Y. Saini, L. K. Shrestha, S. and Svintradze, I. Gronberg, J. Lange, D. Rebassoo, F. and Wright, D. Baden, A. Calvert, B. Eno, S. C. Gomez, J. A. and Hadley, N. J. Kellogg, R. G. Kolberg, T. Lu, Y. and Marionneau, M. Mignerey, A. C. Pedro, K. Skuja, A. and Temple, J. Tonjes, M. B. Tonwar, S. C. Apyan, A. and Barbieri, R. Bauer, G. Busza, W. Cali, I. A. Chan, M. and Di Matteo, L. Dutta, V. Ceballos, G. Gomez Goncharov, M. and Gulhan, D. Klute, M. Lai, Y. S. Lee, Y. -J. Levin, A. Luckey, P. D. Ma, T. Paus, C. Ralph, D. Roland, C. Roland, G. Stephans, G. S. F. Stckli, F. Sumorok, K. and Velicanu, D. Veverka, J. Wyslouch, B. Yang, M. Yoon, A. S. Zanetti, M. Zhukova, V. Dahmes, B. De Benedetti, A. Gude, A. Kao, S. C. Klapoetke, K. Kubota, Y. and Mans, J. Pastika, N. Rusack, R. Singovsky, A. Tambe, N. and Turkewitz, J. Acosta, J. G. Cremaldi, L. M. Kroeger, R. and Oliveros, S. Perera, L. Rahmat, R. Sanders, D. A. and Summers, D. Avdeeva, E. Bloom, K. Bose, S. Claes, D. R. and Dominguez, A. Suarez, R. Gonzalez Keller, J. Knowlton, D. Kravchenko, I. Lazo-Flores, J. Malik, S. Meier, F. and Snow, G. R. Dolen, J. Godshalk, A. Iashvili, I. and Jain, S. Kharchilava, A. Kumar, A. Rappoccio, S. and Alverson, G. Barberis, E. Baumgartel, D. Chasco, M. and Haley, J. Massironi, A. Nash, D. Orimoto, T. Trocino, D. and Wood, D. Zhang, J. Anastassov, A. Hahn, K. A. Kubik, A. Lusito, L. Mucia, N. Odell, N. Pollack, B. and Pozdnyakov, A. Schmitt, M. Stoynev, S. Sung, K. Velasco, M. Won, S. Berry, D. Brinkerhoff, A. Chan, K. M. and Drozdetskiy, A. Hildreth, M. Jessop, C. Karmgard, D. J. and Kellams, N. Kolb, J. Lannon, K. Luo, W. Lynch, S. and Marinelli, N. Morse, D. M. Pearson, T. Planer, M. and Ruchti, R. Slaunwhite, J. Valls, N. Wayne, M. Wolf, M. and Woodard, A. Antonelli, L. Bylsma, B. Durkin, L. S. and Flowers, S. Hill, C. Hughes, R. Kotov, K. Ling, T. Y. and Puigh, D. Rodenburg, M. Smith, G. Vuosalo, C. Winer, B. L. Wolfe, H. Wulsin, H. W. Berry, E. Elmer, P. and Halyo, V. Hebda, P. Hegeman, J. Hunt, A. Jindal, P. and Koay, S. A. Lujan, P. Marlow, D. Medvedeva, T. Mooney, M. Olsen, J. Pirou, P. Quan, X. Raval, A. Saka, H. and Stickland, D. Tully, C. Werner, J. S. Zenz, S. C. and Zuranski, A. Brownson, E. Lopez, A. Mendez, H. Vargas, J. E. Ramirez Alagoz, E. Benedetti, D. Bolla, G. and Bortoletto, D. De Mattia, M. Everett, A. Hu, Z. Jha, M. and Jones, M. Jung, K. Kress, M. Leonardo, N. Pegna, D. Lopes Maroussov, V. Merkel, P. Miller, D. H. Neumeister, N. Radburn-Smith, B. C. Shipsey, I. Silvers, D. and Svyatkovskiy, A. Wang, F. Xie, W. Xu, L. Yoo, H. D. and Zablocki, J. Zheng, Y. Parashar, N. Adair, A. Akgun, B. and Ecklund, K. M. Geurts, F. J. M. Li, W. Michlin, B. and Padley, B. P. Redjimi, R. Roberts, J. Zabel, J. and Betchart, B. Bodek, A. Covarelli, R. De Barbaro, P. and Demina, R. Eshaq, Y. Ferbel, T. Garcia-Bellido, A. and Goldenzweig, P. Han, J. Harel, A. Miner, D. C. Petrillo, G. Vishnevskiy, D. Zielinski, M. Bhatti, A. Ciesielski, R. Demortier, L. Goulianos, K. Lungu, G. Malik, S. and Mesropian, C. Arora, S. Barker, A. Chou, J. P. and Contreras-Campana, C. Contreras-Campana, E. Duggan, D. and Ferencek, D. Gershtein, Y. Gray, R. Halkiadakis, E. and Hidas, D. Lath, A. Panwalkar, S. Park, M. Patel, R. and Rekovic, V. Robles, J. Salur, S. Schnetzer, S. Seitz, C. and Somalwar, S. Stone, R. Thomas, S. Thomassen, P. and Walker, M. Rose, K. Spanier, S. Yang, Z. C. York, A. and Bouhali, O. Eusebi, R. Flanagan, W. Gilmore, J. Kamon, T. Khotilovich, V. Krutelyov, V. Montalvo, R. Osipenkov, I. Pakhotin, Y. Perloff, A. Roe, J. Safonov, A. and Sakuma, T. Suarez, I. Tatarinov, A. Toback, D. Akchurin, N. Cowden, C. Damgov, J. Dragoiu, C. Dudero, P. R. and Kovitanggoon, K. Kunori, S. Lee, S. W. Libeiro, T. and Volobouev, I. Appelt, E. Delannoy, A. G. Greene, S. and Gurrola, A. Johns, W. Maguire, C. Mao, Y. Melo, A. and Sharma, M. Sheldon, P. Snook, B. Tuo, S. Velkovska, J. and Arenton, M. W. Boutle, S. Cox, B. Francis, B. and Goodell, J. Hirosky, R. Ledovskoy, A. Lin, C. Neu, C. and Wood, J. Gollapinni, S. Harr, R. Karchin, P. E. Don, C. Kottachchi Kankanamge Lamichhane, P. Belknap, D. A. and Borrello, L. Carlsmith, D. Cepeda, M. Dasu, S. Duric, S. and Friis, E. Grothe, M. Hall-Wilton, R. Herndon, M. and Herve, A. Klabbers, P. Klukas, J. Lanaro, A. Levine, A. and Loveless, R. Mohapatra, A. Ojalvo, I. Perry, T. and Pierro, G. A. Polese, G. Ross, I. Sakharov, A. Sarangi, T. Savin, A. Smith, W. H. CMS Collaboration

Subjects
High Energy Physics::Phenomenology, High Energy Physics::Experiment
Abstract

Results are presented of a search for a “natural” supersymmetry scenario with gauge mediated symmetry breaking. It is assumed that only the supersymmetric partners of the top quark (the top squark) and the Higgs boson (Higgsino) are accessible. Events are examined in which there are two photons forming a Higgs boson candidate, and at least two b-quark jets. In 19.7 fb(-1) of proton-proton collision data at root s = 8 TeV, recorded in the CMS experiment, no evidence of a signal is found and lower limits at the 95% confidence level are set, excluding the top squark mass below 360 to 410 GeV, depending on the Higgsino mass.

Published
2014

18. Théorie politique

Author

Berner, Christian, Thouard, Denis, Berner, C ( Christian ), Thouard, D ( Denis ), Terrier, Jean, Berner, Christian, Thouard, Denis, Berner, C ( Christian ), Thouard, D ( Denis ), and Terrier, Jean

Published
2015

19. Proform: Profile Forming Innovation

Author

Maria Angeles Gutierrez, I. Eguia, S. Berner, C. Hennigs, A. Sedlmaier, A. Agnello, J. Bahillo

Subjects
ComputerApplications_COMPUTERSINOTHERSYSTEMS
Abstract

IDDRG - Conference Proceedings - Tools and Technologies for the Processing of ultra high strength steels

Published
2010
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30. Mesures par anémométrie laser derrière des corps bi- et tridimensionnels

Author

Jaeggy, B. C., Berner, C., Jaeggy, B. C., and Berner, C.

Abstract

Le but de cet article est d'illustrer par quelques exemples significatifs l'intérêt de l'anémométrie laser pour l'étude des écoulements décollés et en particulier pour l'analyse des caractéristiques instationnaires et turbulentes de ces écoulements. On ne rappellera donc pas ici les principes de la méthode qui sont maintenant bien connus et on ne décrira pas les montages employés, au sujet desquels on voudra bien se reporter à la bibliographie. Les exemples passés en revue concernent la transition par décollement laminaire et recollement turbulent sur un profil d'aile, la couche limite turbulente proche du décollement sur le même profil, l'allée de tourbillons derrière un profil d'aile muni d'un spoiler et enfin le champ d'écoulement derrière un culot cylindrique droit et un culot avec méplats.

Published
1987
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32. Treatment of systemic lupus erythematosus with LJP 394.

Author

Furie, R A, Cash, J M, Cronin, M E, Katz, R S, Weisman, M H, Aranow, C, Liebling, M R, Hudson, N P, Berner, C M, Coutts, S, and de Haan, H A

Abstract

OBJECTIVE: LJP 394 is a novel therapy under development for the treatment of systemic lupus erythematosus (SLE). We investigated the optimal LJP 394 dosing regimen required to maximally reduce serum dsDNA antibodies. We also evaluated the safety and tolerability of repeated doses of LJP 394 as well as the effects of therapy on SLE related disease activity and health related quality of life. METHODS: This was a multicenter, partially randomized, placebo controlled, double blind, dose-ranging trial. Study drug or placebo was administered at weekly, biweekly, or monthly intervals for a total of 17, 9, or 5 doses, respectively. Fifty-eight patients were randomly assigned to receive 1, 10, or 50 mg LJP 394 or placebo. After a 2 month pretreatment period, dosing visits continued for 16 weeks, after which there was a 2 month posttreatment period. RESULTS: The greatest reductions in mean dsDNA antibody titers were observed in the group of patients who received 50 mg LJP 394 weekly (38.1% and 37.1 % at Weeks 16 and 24, respectively). A reduction (29.3%) in dsDNA antibody titers was also observed at Week 24 in the group of patients who received 10 mg LJP 394 weekly. The frequencies of adverse events were comparable in the placebo and active treatment groups. CONCLUSION: This clinical trial, in which a large number of patients with SLE were treated with LJP 394, expanded the safety profile of LJP 394 and demonstrated its capacity to reduce dsDNA antibodies.

Published
2001

33. Laser velocity measurements behind two and three dimensional bodies

Author

Jaeggy, B. and Berner, C.

Abstract

The purpose of this paper is to present, by means of a few significant examples, the benefits of laser Doppler velocimetry (LDV) in the study of the unstationnary and turbulent characteristics in separated flows. The principles of the method is now well known and therefore the LDV systems mentionned in the references will not be described in this paper. Experimental investigations concern the study of the laminar-turbulent transition on an airfoil as well as the turbulent boundary layer near the separation, the vortex street behind an airfoil equipped with a spoiler, and finally the flow field behind a cylindrical afterbody and a triangular boattail.Le but de cet article est d'illustrer par quelques exemples significatifs l'int?r?t de l'an?mom?trie laser pour l'?tude des ?coulements d?coll?s et en particulier pour l'analyse des caract?ristiques instationnaires et turbulentes de ces ?coulements. On ne rappellera donc pas ici les principes de la m?thode qui sont maintenant bien connus et on ne d?crira pas les montages employ?s, au sujet desquels on voudra bien se reporter ? la bibliographie. Les exemples pass?s en revue concernent la transition par d?collement laminaire et recollement turbulent sur un profil d'aile, la couche limite turbulente proche du d?collement sur le m?me profil, l'all?e de tourbillons derri?re un profil d'aile muni d'un spoiler et enfin le champ d'?coulement derri?re un culot cylindrique droit et un culot avec m?plats.

Published
1987

34. Flow Around Baffles

Author

Berner, C., Durst, F., and McEligot, D. M.

Abstract

Flow visualization, manometry, and laser-Doppler anemometry have been applied to approximately two-dimensional water flow around segmental baffles with baffle spacing/depth equal to 0.4, window cuts from 10 to 50 percent, and Reynolds numbers ranging from 600–10,500 in order to simulate important aspects relating to shellside flow in shell-and-tube heat exchangers. The main features of the flow (which is eventually periodic in the streamwise direction), development lengths, pressure loss coefficients, and mean and rms velocity distributions are presented.

Published
1984
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42. Die Exoduserzählung

Author

Berner, Christoph

Subjects
Religion, Biblical Studies, Old Testament, bic Book Industry Communication::H Humanities::HR Religion & beliefs, bic Book Industry Communication::H Humanities::HR Religion & beliefs::HRL Aspects of religion (non-Christian)::HRLC Sacred texts::HRLC1 Criticism & exegesis of sacred texts
Abstract

Christoph Berner legt eine detaillierte redaktionsgeschichtliche Analyse der Exoduserzählung (Ex 1-15) und des mit ihr literarisch eng verknüpften Jitro-Kapitels Ex 18 vor. Dabei zeigt sich, daß das Paradigma der Neueren Urkundenhypothese vollständig zu verabschieden ist. Sämtliche Textanteile lassen sich im Rahmen eines zumeist kleinschrittig verlaufenden Fortschreibungsprozesses erklären, in dem die Exoduserzählung sukzessive Gestalt gewann. Noch in einer vorpriesterschriftlichen Entwicklungsphase wurde sie redaktionell mit der Vätererzählung der Genesis verbunden und damit zum Teil einer Erzväter und Exodus umfassenden Volksgeschichte, der die priesterschriftliche Bearbeitung sodann offenbarungstheologisches Profil verleiht. Hieran anknüpfend entspinnt sich eine komplexe nachpriesterschriftliche Fortschreibungsaktivität, der weite Teile der Exoduserzählung allererst ihre Existenz verdanken. Das alle Phasen der Textgenese einende Prinzip ist die fortwährende 'midraschartige' Selbstauslegung der werdenden Schrift.

Published
2010

43. [Uterine conservative treatment for placenta accreta : new standard?]

Author

Metaxas T, Garcia Vilaplana E, Peter-Berner C, Palacios-Jaraquemada JM, Nieto-Calvache AJ, Martinez De Tejada B, and Pellegrinelli JM

Subjects
Humans, Female, Pregnancy, Cesarean Section methods, Cesarean Section adverse effects, Hysterectomy methods, Telemedicine, Postpartum Hemorrhage therapy, Postpartum Hemorrhage prevention & control, Placenta Accreta therapy, Placenta Accreta diagnosis, Conservative Treatment methods
Abstract

Placenta accreta spectrum (PAS) is among the most dangerous obstetric conditions due to elevated risk of massive hemorrhage. Ultrasound is PAS's preferred screening method. This article -introduces the new topographic classification of PAS, allowing for treatment selection, whether radical or conservative. This classification enables considering the One-Step Conservative Surgery (OSCS), a technique that could potentially avoid up to 80% of cesarean -hysterectomies in cases of PAS. This article details the procedure and -eligibility criteria for the OSCS. This promising technique could significantly reduce maternal morbidity and mortality. Finally, the possibility of training in these techniques through telemedicine opens new avenues for conservative uterine treatment in the -context of PAS., Competing Interests: Les auteurs n’ont déclaré aucun conflit d’intérêts en relation avec cet article.

Published
2024
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44. Sculpting conducting nanopore size and shape through de novo protein design.

Author

Berhanu S, Majumder S, Müntener T, Whitehouse J, Berner C, Bera AK, Kang A, Liang B, Khan N, Sankaran B, Tamm LK, Brockwell DJ, Hiller S, Radford SE, Baker D, and Vorobieva AA

Subjects
Protein Folding, Crystallography, X-Ray, Nuclear Magnetic Resonance, Biomolecular, Protein Conformation, beta-Strand, Models, Molecular, Nanopores, Protein Engineering
Abstract

Transmembrane β-barrels have considerable potential for a broad range of sensing applications. Current engineering approaches for nanopore sensors are limited to naturally occurring channels, which provide suboptimal starting points. By contrast, de novo protein design can in principle create an unlimited number of new nanopores with any desired properties. Here we describe a general approach to designing transmembrane β-barrel pores with different diameters and pore geometries. Nuclear magnetic resonance and crystallographic characterization show that the designs are stably folded with structures resembling those of the design models. The designs have distinct conductances that correlate with their pore diameter, ranging from 110 picosiemens (~0.5 nanometer pore diameter) to 430 picosiemens (~1.1 nanometer pore diameter). Our approach opens the door to the custom design of transmembrane nanopores for sensing and sequencing applications.

Published
2024
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45. Validation of de novo designed water-soluble and transmembrane β-barrels by in silico folding and melting.

Author

Hermosilla AM, Berner C, Ovchinnikov S, and Vorobieva AA

Subjects
Water chemistry, Computer Simulation, Models, Molecular, Protein Conformation, beta-Strand, Deep Learning, Algorithms, Protein Folding, Solubility, Membrane Proteins chemistry
Abstract

In silico validation of de novo designed proteins with deep learning (DL)-based structure prediction algorithms has become mainstream. However, formal evidence of the relationship between a high-quality predicted model and the chance of experimental success is lacking. We used experimentally characterized de novo water-soluble and transmembrane β-barrel designs to show that AlphaFold2 and ESMFold excel at different tasks. ESMFold can efficiently identify designs generated based on high-quality (designable) backbones. However, only AlphaFold2 can predict which sequences have the best chance of experimentally folding among similar designs. We show that ESMFold can generate high-quality structures from just a few predicted contacts and introduce a new approach based on incremental perturbation of the prediction ("in silico melting"), which can reveal differences in the presence of favorable contacts between designs. This study provides a new insight on DL-based structure prediction models explainability and on how they could be leveraged for the design of increasingly complex proteins; in particular membrane proteins which have historically lacked basic in silico validation tools., (© 2024 The Protein Society.)

Published
2024
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46. Sculpting conducting nanopore size and shape through de novo protein design.

Author

Berhanu S, Majumder S, Müntener T, Whitehouse J, Berner C, Bera AK, Kang A, Liang B, Khan GN, Sankaran B, Tamm LK, Brockwell DJ, Hiller S, Radford SE, Baker D, and Vorobieva AA

Abstract

Transmembrane β-barrels (TMBs) are widely used for single molecule DNA and RNA sequencing and have considerable potential for a broad range of sensing and sequencing applications. Current engineering approaches for nanopore sensors are limited to naturally occurring channels such as CsgG, which have evolved to carry out functions very different from sensing, and hence provide sub-optimal starting points. In contrast, de novo protein design can in principle create an unlimited number of new nanopores with any desired properties. Here we describe a general approach to the design of transmembrane β-barrel pores with different diameter and pore geometry. NMR and crystallographic characterization shows that the designs are stably folded with structures close to the design models. We report the first examples of de novo designed TMBs with 10, 12 and 14 stranded β-barrels. The designs have distinct conductances that correlate with their pore diameter, ranging from 110 pS (~0.5 nm pore diameter) to 430 pS (~1.1 nm pore diameter), and can be converted into sensitive small-molecule sensors with high signal to noise ratio. The capability to generate on demand β-barrel pores of defined geometry opens up fundamentally new opportunities for custom engineering of sequencing and sensing technologies.

Published
2023
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47. Combining Molecular Dynamics Simulations and Biophysical Characterization to Investigate Protein-Specific Excipient Effects on Reteplase during Freeze Drying.

Author

Ko SK, Björkengren G, Berner C, Winter G, Harris P, and Peters GHJ

Abstract

We performed molecular dynamics simulations of Reteplase in the presence of different excipients to study the stabilizing mechanisms and to identify the role of excipients during freeze drying. To simulate the freeze-drying process, we divided the process into five distinct steps: (i) protein-excipient formulations at room temperature, (ii) the ice-growth process, (iii)-(iv) the partially solvated and fully dried formulations, and (v) the reconstitution. Furthermore, coarse-grained (CG) simulations were employed to explore the protein-aggregation process in the presence of arginine. By using a coarse-grained representation, we could observe the collective behavior and interactions between protein molecules during the aggregation process. The CG simulations revealed that the presence of arginine prevented intermolecular interactions of the catalytic domain of Reteplase, thus reducing the aggregation propensity. This suggests that arginine played a stabilizing role by interacting with protein-specific regions. From the freeze-drying simulations, we could identify several protein-specific events: (i) collapse of the domain structure, (ii) recovery of the drying-induced damages during reconstitution, and (iii) stabilization of the local aggregation-prone region via direct interactions with excipients. Complementary to the simulations, we employed nanoDSF, size-exclusion chromatography, and CD spectroscopy to investigate the effect of the freeze-drying process on the protein structure and stability.

Published
2023
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48. Crossing religious boundaries: Individual and contextual determinants of who can violate religious norms.

Author

Dahl A, Berner C, Jesuthasan J, Wehry J, and Srinivasan M

Subjects
Adolescent, Adult, Humans, United States, Judgment, Religion
Abstract

In pluralistic societies, encounters with individuals, contexts, and norms of other religions can prompt conflict. We test a novel framework for explaining how individuals apply religious norms across individuals and contexts. In Studies 1 and 2, adolescents and adults in India and the United States judged events in which religious norms were violated by protagonists of different religions in different religious contexts. Participants often judged that norm violations were wrong even when the norm religion matched only the protagonist or context religion. Study 3 presented dilemmas that pitted religious norms against non-religious concerns. Participants favored following the religious norm yet accepted the protagonist's right to violate it. In each adult sample, more religious participants more often judged that protagonists were obligated to follow the protagonist's own religious norms. These findings reveal individual and contextual determinants of judgments about religious violations with implications for peaceful coexistence in pluralistic societies., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Published
2022
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49. Investigation of the pH-dependent aggregation mechanisms of GCSF using low resolution protein characterization techniques and advanced molecular dynamics simulations.

Author

Ko SK, Berner C, Kulakova A, Schneider M, Antes I, Winter G, Harris P, and Peters GHJ

Abstract

Granulocyte-colony stimulating factor (GCSF) is a widely used therapeutic protein to treat neutropenia. GCSF has an increased propensity to aggregate if the pH is increased above 5.0. Although GCSF is very well experimentally characterized, the exact pH-dependent aggregation mechanism of GCSF is still under debate. This study aimed to model the complex pH-dependent aggregation behavior of GCSF using state-of-the-art simulation techniques. The conformational stability of GCSF was investigated by performing metadynamics simulations, while the protein-protein interactions were investigated using coarse-grained (CG) simulations of multiple GCSF monomers. The CG simulations were directly compared with small-angle X-ray (SAXS) data. The metadynamics simulations demonstrated that the orientations of Trp residues in GCSF are dependent on pH. The conformational change of Trp residues is due to the loss of Trp-His interactions at the physiological pH, which in turn may increase protein flexibility. The helical structure of GCSF was not affected by the pH conditions of the simulations. Our CG simulations indicate that at pH 4.0, the colloidal stability may be more important than the conformational stability of GCSF. The electrostatic potential surface and CG simulations suggested that the basic residues are mainly responsible for colloidal stability as deprotonation of these residues causes a reduction of the highly positively charged electrostatic barrier close to the aggregation-prone long loop regions., Competing Interests: The authors declare that there are no known conflicts of interest. The financial support of the research or personal relationships do not have any significant impact on the outcomes reported in this works., (© 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.)

Published
2022
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50. Combining Unfolding Reversibility Studies and Molecular Dynamics Simulations to Select Aggregation-Resistant Antibodies.

Author

Berner C, Menzen T, Winter G, and Svilenov HL

Subjects
Antibodies, Monoclonal therapeutic use, Calorimetry, Differential Scanning, Chemistry, Pharmaceutical methods, Chromatography, Gel, Drug Storage, Dynamic Light Scattering, Hot Temperature adverse effects, Hydrogen-Ion Concentration, Molecular Dynamics Simulation, Protein Conformation, Protein Folding, Protein Unfolding, Antibodies, Monoclonal chemistry, Protein Denaturation
Abstract

The efficient development of new therapeutic antibodies relies on developability assessment with biophysical and computational methods to find molecules with drug-like properties such as resistance to aggregation. Despite the many novel approaches to select well-behaved proteins, antibody aggregation during storage is still challenging to predict. For this reason, there is a high demand for methods that can identify aggregation-resistant antibodies. Here, we show that three straightforward techniques can select the aggregation-resistant antibodies from a dataset with 13 molecules. The ReFOLD assay provided information about the ability of the antibodies to refold to monomers after unfolding with chemical denaturants. Modulated scanning fluorimetry (MSF) yielded the temperatures that start causing irreversible unfolding of the proteins. Aggregation was the main reason for poor unfolding reversibility in both ReFOLD and MSF experiments. We therefore performed temperature ramps in molecular dynamics (MD) simulations to obtain partially unfolded antibody domains in silico and used CamSol to assess their aggregation potential. We compared the information from ReFOLD, MSF, and MD to size-exclusion chromatography (SEC) data that shows whether the antibodies aggregated during storage at 4, 25, and 40 °C. Contrary to the aggregation-prone molecules, the antibodies that were resistant to aggregation during storage at 40 °C shared three common features: (i) higher tendency to refold to monomers after unfolding with chemical denaturants, (ii) higher onset temperature of nonreversible unfolding, and (iii) unfolding of regions containing aggregation-prone sequences at higher temperatures in MD simulations.

Published
2021
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