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1. Antithrombotic activity of flavonoid fractions of Juglans regia root bark through inhibiting platelet aggregation and plasmatic coagulation

Author

AMIROU, Asmae, LEGSSYER, Abdelkhaleq, ZIYYAT, Abderrahim, AZIZ, Mohammed, BNOUHAM, Mohamed, BERRABAH, Mohammed, MARKAOUI, Mostafa, BENSAID, Mohammed, ZAID, Younes, and MEKHFI, Hassane

Subjects
Acute thromboembolism, Coagulation, Flavonoids, Juglans regia, Platelet aggregation
Abstract

Thrombus formation in cardiovascular diseases is one of the important mechanisms responsible for death and disability. Plants have long been used as traditional remedies for the treatment of many diseases. In this study, we investigated the antithrombotic, antiaggregant, and anticoagulant activities of flavonoids isolated from Juglans regia root bark. The antithrombotic activity was evaluated on collagen plus epinephrine induced acute pulmonary thromboembolism model of mice and murine platelet aggregation was induced by different agonists. Clotting parameters including activated partial thromboplastin time, prothrombin time, and thrombin time in the platelet-poor plasma were determined in vitro by using a coagulometer in the presence and absence of flavonoid fractions. Flavonoids extracted from Juglans regia exhibited an antithrombotic activity (80% of protection) by preventing the paralysis or death induced by an intravenous injection of collagen and epinephrine. In addition, these compounds significantly inhibited platelet aggregation and prolonged the plasmatic coagulation time. Flavonoids isolated from Juglans regia exert a potent antithrombotic effect via antiaggregant and anticoagulant actions. Juglans regia could be a precious source of antithrombotic compounds., Arabian Journal of Medicinal and Aromatic Plants, مجلد 8, عدد 3 (2022)

Published
2022
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3. Antithrombotic, Antiaggregant and Anticoagulant Effect of Methanolic Fraction of Calamintha officinalis: In vitro and ex vivo Experiments

Author

Zoheir, Kaoutar; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Bnouham, Mohamed; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Legssyer, Abdelkhaleq; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Ziyyat, Abderrahim; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Berrabah, Mohammed; Laboratory of Mineral Solid and Analytical Chemistry, Department of Chemistry, Faculty of Sciences, University Mohammed the First, Oujda 60000, Aziz, Mohammed; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Bensaid, Mohammed; Laboratory Bensaid of Pathological Cytology and Anatomy, 100, Allal Ben Abdellah Street, Apartment number 9, Oujda, 60000, Mekhfi, Hassane; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Zoheir, Kaoutar; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Bnouham, Mohamed; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Legssyer, Abdelkhaleq; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Ziyyat, Abderrahim; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Berrabah, Mohammed; Laboratory of Mineral Solid and Analytical Chemistry, Department of Chemistry, Faculty of Sciences, University Mohammed the First, Oujda 60000, Aziz, Mohammed; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000, Bensaid, Mohammed; Laboratory Bensaid of Pathological Cytology and Anatomy, 100, Allal Ben Abdellah Street, Apartment number 9, Oujda, 60000, and Mekhfi, Hassane; Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, University Mohammed the First, Oujda, 60000

Abstract

The objective was to study the effect of Calamintha officinalis methanolic fraction in the prevention of thrombosis and to explore its effect on primary and secondary hemostasis. The antithrombotic activity was evaluated by using the pulmonary thromboembolism experimental model. Platelet aggregation, tail bleeding time and coagulation assays were explored to elucidate the antithrombotic mechanism. For aggregation, washed platelets were incubated in presence of Calamintha officinalis methanolic fraction and then activated by different agonists (thrombin, ADP, collagen). The bleeding time was determined to measure the time between the tail incision and the moment the bleeding stops. The coagulation was explored by measuring prothrombin time, activated partial thromboplastin time, thrombin time and plasmatic fibrinogen concentration. For ex vivo study, the animals were treated by the Calamintha officinalis methanolic fraction (250 mg/kg/day) for 4 weeks. Then, platelet aggregation, tail bleeding time and coagulation parameters was studied. The results indicate that C. officinalis methanol fraction seems to exert in vivo an antithrombotic action (40% of protection). The in vitro investigations also show that this fraction significantly inhibits aggregation, increases bleeding time, coagulation times and reduces fibrinogen concentration. The chronic treatment of animals confirms the precedent data. This study demonstrates an antithrombotic effect of C. officinalis. The observed antiaggregant and anticoagulant effects could explain this activity.

Published
2018

4. Antithrombotic, Antiaggregant and Anticoagulant Effect of Methanolic Fraction of Calamintha officinalis: In vitro and ex vivo Experiments

Author

Zoheir, Kaoutar, Bnouham, Mohamed, Legssyer, Abdelkhaleq, Ziyyat, Abderrahim, Berrabah, Mohammed, Aziz, Mohammed, Bensaid, Mohammed, Mekhfi, Hassane, Zoheir, Kaoutar, Bnouham, Mohamed, Legssyer, Abdelkhaleq, Ziyyat, Abderrahim, Berrabah, Mohammed, Aziz, Mohammed, Bensaid, Mohammed, and Mekhfi, Hassane

Abstract

The objective was to study the effect of Calamintha officinalis methanolic fraction in the prevention of thrombosis and to explore its effect on primary and secondary hemostasis. The antithrombotic activity was evaluated by using the pulmonary thromboembolism experimental model. Platelet aggregation, tail bleeding time and coagulation assays were explored to elucidate the antithrombotic mechanism. For aggregation, washed platelets were incubated in presence of Calamintha officinalis methanolic fraction and then activated by different agonists (thrombin, ADP, collagen). The bleeding time was determined to measure the time between the tail incision and the moment the bleeding stops. The coagulation was explored by measuring prothrombin time, activated partial thromboplastin time, thrombin time and plasmatic fibrinogen concentration. For ex vivo study, the animals were treated by the Calamintha officinalis methanolic fraction (250 mg/kg/day) for 4 weeks. Then, platelet aggregation, tail bleeding time and coagulation parameters was studied. The results indicate that C. officinalis methanol fraction seems to exert in vivo an antithrombotic action (40% of protection). The in vitro investigations also show that this fraction significantly inhibits aggregation, increases bleeding time, coagulation times and reduces fibrinogen concentration. The chronic treatment of animals confirms the precedent data. This study demonstrates an antithrombotic effect of C. officinalis. The observed antiaggregant and anticoagulant effects could explain this activity.

Published
2018

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