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Seasonal variation of proximate composition (moisture, protein, fat and ash) and biochemical parameter like peroxide value (PV), Total Volatile Base Nitrogen (TVB-N), Trimethylamine Nitrogen (TMA-N) and Potential of Hydrogen (pH) of Rastrelliger kanagurta was analyzed from Diamond Harbour fish landing center, West Bengal, East coast of India. The result show on that the proximate and biochemical composition of fish depends on season. There were moisture content was found to be in the range of 72.56±0.11% and 79.09±0.08%. The protein content was found to be ranging between 16.59±0.28% to 19.27±0.15%. Higher fat content was recorded during breeding season and lower during spent phase or post breeding season. There were no significant changes (p>0.05) in the values of ash within the various months with higher values seen during the dry season. The PV recorded higher values mostly in the last quarter of the year and lower values in the first quarter. TVB-N and TMA-N values of fresh landed fish were found ranging between 19.52±0.04 to 23.02±0.04 mg/100g flesh and 9.64±0.06 to 12.37±0.07 mgN/100g flesh, respectively. The values of pH of the samples ranged from 6.30±0.04 to 7.21±0.01. [ABSTRACT FROM AUTHOR]

Background: Several natural/synthetic molecules having a structure similar to 1H-isochromen- 1-ones have been reported to display promising antioxidants and platelet aggregation inhibitory activity. Isocoumarin (1H-2-benzopyran-1-one) skeleton, either whole or as a part of the molecular framework, has been explored for its antioxidant or antiplatelet activities., Introduction: Based on the literature, a new prototype, i.e., 3-phenyl-1H-isochromen-1-ones based compounds, has been rationalized to possess both antioxidant as well as antiplatelet activities. Consequently, no reports are available regarding its inhibition either by cyclooxygenase-1 (COX-1) enzyme or by arachidonic acid (AA)-induced platelet aggregation. This prompted us to investigate 3-phenyl-1H-isochromen-1-ones towards antioxidant and antiplatelet agents., Methods: The goal of this work was to identify new 3-phenyl-1H-isochromen-1-ones based compounds via synthesis of a series of analogues, followed by performing in vitro antioxidant as well as AA-induced antiplatelet activities. Then, identification of potent compounds by SAR and molecular docking studies was carried out., Results: Out of all synthesized 3-phenyl-1H-isochromen-1-ones analogues, five compounds showed 7-fold to 16-fold more highly potent antioxidant activities than ascorbic acid. Altogether, ten 3-phenyl-1H-isochromen- 1-one analogues displayed antioxidant activities in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Almost all the 3-phenyl-1H-isochromen-1-one analogues exhibited potent AA-induced antiplatelet activity; few of them displayed 7-folds more activity as compared to aspirin. Further, in silico analysis validated the wet results., Conclusion: We disclose the first detailed study for the identification of 3-phenyl-1H-isochromen-1-one analogues as highly potent antioxidant as well as antiplatelet agents. The article describes the scaffold designing, synthesis, bioevaluation, structure-activity relationship, and in silico studies of a pharmaceutically privileged bioactive 3-phenyl-1H-isochromen-1-one class of heterocycles., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)