93 results on '"Bian, Bao-Lin"'
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2. The integrated study on the chemical profiling and in vivo course to explore the bioactive constituents and potential targets of Chinese classical formula Qingxin Lianzi Yin Decoction by UHPLC-MS and network pharmacology approaches
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Gao, Wen-Ya, Si, Nan, Li, Ming-li, Gu, Xin-ru, Zhang, Yan, Zhou, Yan-yan, Wang, Hong-Jie, Wei, Xiao-Lu, Bian, Bao-Lin, and Zhao, Hai-Yu
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- 2021
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3. The lipid homeostasis regulation study of arenobufagin in zebrafish HepG2 xenograft model and HepG2 cells using integrated lipidomics-proteomics approach
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Zhao, Li-Juan, Zhao, Hai-Yu, Wei, Xiao-Lu, Guo, Fei-Fei, Wei, Jun-Ying, Wang, Hong-Jie, Yang, Jian, Yang, Zhi-Gang, Si, Nan, and Bian, Bao-Lin
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- 2020
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4. Study on the potential mechanism of Qingxin Lianzi Yin Decoction on renoprotection in db/db mice via network pharmacology and metabolomics
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Gao, Wen-ya, primary, Tian, Meng-yao, additional, Li, Ming-li, additional, Gao, Shuang-rong, additional, Wei, Xiao-lu, additional, Gao, Chang, additional, Zhou, Yan-yan, additional, Li, Tao, additional, Wang, Hong-jie, additional, Bian, Bao-lin, additional, Si, Nan, additional, Zhao, Wei, additional, and Zhao, Hai-yu, additional
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- 2023
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5. Chemical profiling and quantification of Chinese medicinal formula Huang-Lian-Jie-Du decoction, a systematic quality control strategy using ultra high performance liquid chromatography combined with hybrid quadrupole-orbitrap and triple quadrupole mass spectrometers
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Yang, Yang, Wang, Hong-Jie, Yang, Jian, Brantner, Adelheid H., Lower-Nedza, Agnieszka D., Si, Nan, Song, Jian-Fang, Bai, Bing, Zhao, Hai-Yu, and Bian, Bao-Lin
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- 2013
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6. Action mechanisms of complementary and alternative medicine therapies for rheumatoid arthritis
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Keisuke, Imada, Bian, Bao-lin / 边宝林, Li, Xiang-dong / 李向东, Takashi, Sato, and Akira, Ito
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- 2011
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7. A Newly Discovered Phenylethanoid Glycoside from Stevia rebaudiana Bertoni Affects Insulin Secretion in Rat INS-1 Islet β Cells
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He, Jing, primary, Zhu, Nai-Liang, additional, Kong, Jing, additional, Peng, Ping, additional, Li, Lin-Fu, additional, Wei, Xiao-Lu, additional, Jiang, Yan-Yan, additional, Zhang, Yan-Ling, additional, Bian, Bao-Lin, additional, She, Gai-Mei, additional, and Shi, Ren-Bing, additional
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- 2019
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8. Pharmacokinetic-Pharmacodynamic Analysis on Inflammation Rat Model after Oral Administration of Huang Lian Jie Du Decoction
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Ren, Wei, primary, Zuo, Ran, additional, Wang, Yao-Nan, additional, Wang, Hong-Jie, additional, Yang, Jian, additional, Xin, Shao-Kun, additional, Han, Ling-Yu, additional, Zhao, Hai-Yu, additional, Han, Shu-Yan, additional, Gao, Bo, additional, Hu, Hao, additional, Hu, Yuan-Jia, additional, Bian, Bao-Lin, additional, and Si, Nan, additional
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- 2016
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9. Comparative metabolism of four limonoids in human liver microsomes using ultra-high-performance liquid chromatography coupled with high-resolution LTQ-Orbitrap mass spectrometry
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Ren, Wei, primary, Xin, Shao-Kun, additional, Han, Ling-Yu, additional, Zuo, Ran, additional, Li, Yan, additional, Gong, Mu-Xing, additional, Wei, Xiao-Lu, additional, Zhou, Yan-Yan, additional, He, Jing, additional, Wang, Hong-Jie, additional, Si, Nan, additional, Zhao, Hai-Yu, additional, Yang, Jian, additional, and Bian, Bao-Lin, additional
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- 2015
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10. Comparative metabolism of tussilagone in rat and human liver microsomes using ultra-high-performance liquid chromatography coupled with high-resolution LTQ-Orbitrap mass spectrometry
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Zhang, Xin-Shi, primary, Ren, Wei, additional, Bian, Bao-Lin, additional, Zhao, Hai-Yu, additional, and Wang, Shu, additional
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- 2015
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11. Metabolic fate analysis of Huang–Lian–Jie–Du Decoction in rat urine and feces by LC–IT-MS combining with LC–FT-ICR-MS: a feasible strategy for the metabolism study of Chinese medical formula
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Zuo, Ran, primary, Ren, Wei, additional, Bian, Bao-Lin, additional, Wang, Hong-Jie, additional, Wang, Yao-Nan, additional, Hu, Hao, additional, Zhao, Hai-Yu, additional, and Si, Nan, additional
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- 2015
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12. Inhibiton of cytochrome P450 isoenzymes and P-gp activity by multiple extracts of Huang-Lian-Jie-Du decoction
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Wang, Ying, primary, Jiang, Yi-ming, additional, Wang, Yong-tao, additional, Kang, Jun-wei, additional, Yu, Tao, additional, Zhao, Hai-Yu, additional, Bian, Bao-Lin, additional, Huang, Min, additional, and Bi, Hui-Chang, additional
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- 2014
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13. Species‐related difference between limonin and obacunone among five liver microsomes and zebrafish using ultra‐high‐performance liquid chromatography coupled with a LTQ‐Orbitrap mass spectrometer
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Ren, Wei, primary, Li, Yan, additional, Zuo, Ran, additional, Wang, Hong‐Jie, additional, Si, Nan, additional, Zhao, Hai‐Yu, additional, Han, Ling‐Yu, additional, Yang, Jian, additional, and Bian, Bao‐Lin, additional
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- 2014
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14. Metabolic fate analysis of Huang–Lian–Jie–Du Decoction in rat urine and feces by LC–IT-MS combining with LC–FT-ICR-MS: a feasible strategy for the metabolism study of Chinese medical formula.
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Zuo, Ran, Ren, Wei, Bian, Bao-Lin, Wang, Hong-Jie, Wang, Yao-Nan, Hu, Hao, Zhao, Hai-Yu, and Si, Nan
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METABOLISM ,COPROPHAGIA ,FLAVONOIDS ,IRIDOIDS ,BIOCHEMISTRY - Abstract
1. Huang–Lian–Jie–Du Decoction (HLJDD) is widely used for the treatment of hypertension, diabetes, inflammation and neural system diseases in clinic. In the present study, the comprehensive metabolic profile of HLJDD was demonstrated reliably and rapidly followed by the metabolic pathway analysis of six typical pure compounds (four alkaloids, one flavonoid and one iridoid) in HLJDD using LC–IT-MS combined with high resolution LC–FT-ICR-MS. 2. Totally, 85 compounds, including 32 prototype components and 53 biotransformed metabolites were detected and characterized in the urine and feces after oral administration of HLJDD and six pure compounds to rats, respectively. Among them, 17 prototypes were identified definitely with standard references. 3. Hydroxylation, demethylation and glucuronidation reactions of alkaloids, as well as glucuronidation and sulfonation reactions of iridoids and flavonoids, were observed as the major metabolic pathways of HLJDD. Flavonoids, iridoids and their metabolites were mainly excreted from urine. However, amount of alkaloids were detected in feces. 4. In general, the distinctive metabolic process of three kinds of representative components in HLJDD was clarified. Thein vivometabolic network of HLJDD was demonstrated. Meanwhile, the investigation of representative pure compounds in metabolic study provided a valuable strategy to elucidate the full-scale metabolic fate of HLJDD. This might be helpful to understand thein vivomechanism of Traditional Chinese medicine (TCM). [ABSTRACT FROM AUTHOR]
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- 2016
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15. Huanglian Jiedu decoction active fraction protects ipsilateral thalamus injury in MCAO rats through regulating astrocytes.
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ZHAO Hui, LONG Jian-fei, ZOU Hai-yan, ZHANG Qiu-xia, ZHANG Jian, WANG-Lei, ZHANG Nan, BIAN Bao-lin, and ZHAO Hai-yu
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- 2014
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16. A new compound from Gastrodia elata blume
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Xiao, Yong-Qing, primary, Li, Li, additional, You, Xiao-Lin, additional, Bian, Bao-Lin, additional, Liang, Xin-Miao, additional, and Wang, Yi-Tao, additional
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- 2002
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17. Study on action mechanism of adjuvant therapeutic effect compound Ejiao slurry in treating cancers based on network pharmacology.
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XU Hai-yu, WANG Song-song, YANG Hong-jun, BIAN Bao-lin, TIAN Shou-sheng, WANG Dong-Hang, LU Peng, ZHOU Xiang-shan, YOU Jin-hua, and HUANG Lu-qi
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- 2014
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18. Synthesis and Magnetic Properties of μ-Oxamido Benuclear Cu(II)-NI(II) Complexes
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Jin, Cheng-Yong, primary, Gao, Jian, additional, Bian, Bao-Lin, additional, Wang, Feng-Ling, additional, Ma, Shu-Lin, additional, and Liao, Dai-Zheng, additional
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- 1996
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19. Chemical Profiling of the Chinese Herb Formula Xiao-Cheng-Qi Decoction Using Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrometry.
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Zhao, Hai-Yu, Fan, Miao-Xuan, Wu, Xu, Wang, Hong-Jie, Yang, Jian, Si, Nan, and Bian, Bao-Lin
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CHINESE medicine ,HERBAL medicine ,LIQUID chromatography ,ELECTROSPRAY ionization mass spectrometry ,ANALYTICAL chemistry ,RHUBARB ,ANTHRAQUINONES - Abstract
An approach was established to analyze the chemical profiling of Xiao-Cheng-Qi Decoction (XCQD) using liquid chromatography coupled with electrospray ionization tandem mass spectrometry. XCQD consisted of three herbal medicines (Rhubarb, Fructus Aurantii Immaturus and Cortex Magnoliae Officinalis). The traditional water extractive method was applied in the sample preparation, which was identical with clinical use. The characteristic fragmentation pathways of 17 reference compounds were comprehensively studied, including precursors of tannins, flavonones, anthraquinones and lignan. In total, 71 constituents were identified or tentatively characterized based on their mass spectrometry fragmentations and chromatographic behaviors. By comparing their relative contents, flavanones and anthraquinones were supposed to be used together for the quality control of XCQD. Further pharmacology and pharmacokinetics investigations should be performed on the basis of the present chemical profiling study. [ABSTRACT FROM PUBLISHER]
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- 2013
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20. [Evaluation of efficacy of Dihuang Baoyuan Granules for diabetes mellitus and composition and in vivo distribution analysis based on UHPLC-LTQ-Orbitrap MS].
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Tian MY, Wang MX, Luo KK, Hu TB, Li HM, Huo SH, Hao LY, Si N, Gao WY, Liu YY, Bian BL, Wang HJ, and Zhou YY
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- Chromatography, High Pressure Liquid methods, Animals, Rats, Male, Humans, Liver drug effects, Liver chemistry, Liver metabolism, Mass Spectrometry methods, Kidney drug effects, Kidney chemistry, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Experimental blood, Diabetes Mellitus drug therapy, Drugs, Chinese Herbal chemistry, Drugs, Chinese Herbal pharmacokinetics, Rats, Sprague-Dawley
- Abstract
Dihuang Baoyuan Granules is a prescription endorsed by HU Tianbao, a renowned and elderly Chinese medicine practitioner from Beijing, and has demonstrated definite clinical efficacy. The composition of this prescription is intricate as it includes 7 distinct herbal medicines. This study aims to analyze the chemical composition of Dihuang Baoyuan Granules, evaluate its efficacy in the treatment of diabetes and analyze the distribution of the drug components in the plasma, liver, and kidney after administration. The findings will serve as a reference for future research on pharmacodynamic substances of this prescription. UHPLC-LTQ-Orbitrap MS was employed to analyze the main chemical components of Dihuang Baoyuan Granules. A Waters ACQUITY Premier HSS T3 column(2.1 mm×100 mm, 1.8 μm) was used for chromatographic separation with 0.1% formic acid(A)-acetonitrile(B) as the mobile phases in a gradient elution at a flow rate of 0.3 mL·min~(-1). Electrospray ionization(ESI) source was used to acquire data in positive and negative ion modes. Furthermore, a rat model of diabetes mellitus was established by feeding with a high-sugar high-fat diet, and injection with streptozocin at a dose of 35 mg·kg~(-1), and the modeled rats were then administrated with Dihuang Baoyuan Granules. The fasting blood glucose, hemoglobin A1c, and other relevant indicators were measured, and the substances present in the plasma, liver, and kidney were identified. By reference to quasi-molecular ions, MS/MS fragment ions, MS spectra of reference substances, and compound information in available reports, 191 components were identified in Dihuang Baoyuan Granules, including 29 alkaloids, 24 flavonoids, 22 organic acids, 16 amino acids, 12 terpenes, 11 steroid saponins, 9 sugars, 8 phenylethanoid glycosides, 8 nucleosides, 2 phenylpropanoids, and 49 others compounds. Eighty-three chemical components were identified in rat plasma, 109 in the liver, and 98 in the kidney. Component identification and characterization of Dihuang Baoyuan Granules in vitro and in vivo provide efficacy information and guidance for the basic research on the pharmacodynamic substances and further clinical application of this prescription.
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- 2024
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21. [Synthesis and tissue distribution of bufadienolides in Bufo bufo gargarizans].
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Liu YY, Sun B, Zhao HY, Ding YH, Zhang Y, Gao WY, Tian MY, Wang HJ, Zhou YY, Wei XL, Si N, and Bian BL
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- Animals, Bufo bufo, Tissue Distribution, Bufonidae, Spectrometry, Mass, Electrospray Ionization, Bufanolides
- Abstract
This study explored the biosynthesis of bufadienolides(BDs) in Bufo bufo gargarizans to solve the dilemma of the decreasing resources of B. bufo gargarizans and provide a theoretical basis for the sustainable utilization of the resources. Ultra-high performance liquid chromatography-Orbitrap-mass spectrometry(UHPLC-Orbitrap-MS) was employed to detect the synthesis sites of BDs in B. bufo gargarizans, and the results were verified by desorption electrospray ionization-mass spectrometry imaging(DESI-MSI) and homogenate incubation experiments. BDs in B. bufo gargarizans had the highest content in the liver and the highest concentration in the gallbladder, in addition to the parotid gland and skin, which suggested that the liver could synthesize BDs. The results of DESI-MSI also showed that BDs were mainly enriched in the liver rather than the immature parotid gland. The incubation experiment of liver homogenates demonstrated the liver of B. bufo gargarizans had the ability to synthesize BDs. This study showed that the liver was a major organ for the synthesis of BDs in B. bufo gargarizans during metamorphosis, development, and growth, which provided strong theoretical support for the biosynthesis of BDs and the sustainable utilization of B. bufo gargarizans resources.
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- 2024
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22. [Component profile analysis of Sanhan Huashi Formula based on UHPLC-LTQ-Orbitrap-MS, GC-MS, and UHPLC-QQQ-MS/MS].
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Wang MX, Yao CS, Zhao HY, Wang WH, Zhang Y, Luo KK, Si N, Bian BL, Li T, Wei XL, Tian MY, Zhou YY, Wang HJ, and Li M
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- Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Reproducibility of Results, Tandem Mass Spectrometry, Drugs, Chinese Herbal chemistry
- Abstract
This study comprehensively analyzed the active components of Sanhan Huashi Formula using qualitative and quantitative mass spectrometry techniques, laying the foundation for understanding its pharmacological substance basis. UHPLC-LTQ-Orbitrap-MS and GC-MS technologies were used to analyze and identify the volatile and non-volatile components in Sanhan Huashi Formula. UHPLC-QQQ-MS/MS technology was used to simultaneously determine the content of 27 major active components in the formula. The results showed that 308 major chemical components were identified in Sanhan Huashi Formula, among which 60 compounds were identified by comparing with reference standards, mainly including alkaloids, flavonoids, coumarins, triterpenoid saponins, amino acids, and nucleosides. GC-MS technology preliminarily identified 52 volatile compounds, with γ-eudesmol and β-eudesmol as the main components. The quantitative results demonstrated good linearity(r>0.99) for the 27 active components, indicating the stability, simplicity, and reliability of the established method. Among them, amygdalin, nodakenin, arecoline, ephedrine, and pseudoephedrine had relatively high content and were presumably the main pharmacologically active substances. In conclusion, this study systematically and comprehensively characterized the major chemical components and patterns in Sanhan Huashi Formula, providing a basis for understanding its pharmacological mechanisms and clinical applications.
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- 2023
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23. [Study on biomarkers of acteoside in treating puromycin aminonucleoside nephropathy in young rats based on non-targeted urine metabolomics technology].
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Wang MX, Luo KK, Gao WY, Tian MY, Zhao HY, Si N, Bian BL, Wei XL, Wang HJ, and Zhou YY
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- Humans, Child, Rats, Animals, Metabolomics methods, Biomarkers urine, Chromatography, High Pressure Liquid methods, Acetophenones, Phenylalanine, Amino Acids, Puromycin Aminonucleoside, Glomerulonephritis
- Abstract
This study aims to reveal the endogenous metabolic characteristics of acteoside in the young rat model of purinomycin aminonucleoside nephropathy(PAN) by non-targeted urine metabolomics and decipher the potential mechanism of action. Biochemical indicators in the urine of rats from each group were determined by an automatic biochemical analyzer. The potential biomarkers and related core metabolic pathways were identified by ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry(UHPLC-LTQ-Orbitrap MS) combined with principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA). MetaboAnalyst 5.0 was used to establish the receiver operating characteristic(ROC) curve for evaluating the clinical diagnostic performance of core metabolites. The results showed that acteoside significantly decreased urinary protein-to-creatinine ratio in PAN young rats. A total of 17 differential metabolites were screened out by non-targeted urine metabolomics in PAN young rats and they were involved in phenylalanine metabolism and phenylalanine, tyrosine and tryptophan biosynthesis. Thirtten differential metabolites were screened by acteoside intervention in PAN young rats, and they were involved in phenylalanine metabolism and arginine and proline metabolism. Among them, leucylproline and acetophenone were the differential metabolites that were significantly recovered after acteoside treatment. These pathways suggest that acteoside treats PAN in young rats by regulating amino acid metabolism. The area under the curve of two core biomarkers, leucylproline and acetophenone, were both greater than 0.9. In summary, acteoside may restore amino acid metabolism by regulating endogenous differential metabolites in PAN young rats, which will help to clarify the mechanism of acteoside in treating chronic glomerulonephritis in children. The characteristic biomarkers screened out have a high diagnostic value for evaluating the treatment of chronic glomerulonephritis in children with acteoside.
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- 2023
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24. [Tissue distribution of Qingfei Paidu Decoction based on HPLC-MS/MS].
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Zhang Y, Zhao HY, Yang LX, Zhou YY, Bian BL, Wu HK, Zhu HY, Si N, Lin PF, Wang L, and Wang HJ
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- Chromatography, High Pressure Liquid, Tissue Distribution, Tandem Mass Spectrometry, Drugs, Chinese Herbal
- Abstract
The tissue distribution of Qingfei Paidu Decoction was studied by HPLC-MS/MS in vivo. Hypersil GOLD C_(18) column(2.1 mm×50 mm, 1.9 μm) was used for gradient elution with acetonitrile as the mobile phase A and 0.1% formic acid solution as the mobile phase B. High-resolution liquid chromatography-mass spectrometry in both positive and negative ion scanning mode and multiple response monitoring(MRM) mode was employed to analyze the behaviors of the active components of Qingfei Paidu Decoction in diffe-rent tissues. The results showed that 19, 9, 17, 14, 22, 19, 24, and 2 compounds were detected in plasma, heart, liver, spleen, lung, kidney, large intestine, and brain, respectively. The compounds belonged to 8 groups, covering 14 herbs in the prescription. After administration with Qingfei Paidu Decoction, the compounds were rapidly distributed in various tissues, especially in the lung, liver, large intestine, and kidney. The majority of the compounds displayed secondary distribution. This study comprehensively analyzed the distribution rules of the main active components in Qingfei Paidu Decoction and provided a basis for the clinical application.
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- 2023
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25. [Systematic comparison of two kinds of Bufonis Venenum derived from different Bufo gargarizans subspecies based on metabolomics and antitumor activity].
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Sun B, Li ML, Ding YH, Zhang Y, Xia B, Guo SY, Wang SL, Bian BL, Si N, and Zhao HY
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- Animals, Bufonidae, Chromatography, High Pressure Liquid, Quality Control, Cell Line, Tumor, Zebrafish, Bufanolides analysis
- Abstract
This paper compared the differences between two kinds of Bufonis Venenum produced by Bufo gargarizans gargarizans and B. gararizans andrewsi, and verified the rationality of the market value orientation of Bufonis Venenum based on the zebrafish mo-del. Twenty batches of Bufonis Venenum from Jiangsu province, Hebei province, Liaoning province, Jilin province, and Liangshan, Sichuan province, including B. gargarizans gargarizans and B. gararizans andrewsi, were collected. The UHPLC-LTQ-Orbitrap-MS combined with principal component analysis was used to compare the differences between two kinds of Bufonis Venenum. According to the limiting conditions of VIP>1, FC<0.5 or FC>2.0, and peak total area ratio>1%, 9 differential markers were determined, which were cinobufagin, cinobufotalin, arenobufagin, resibufogenin, scillaredin A, resibufagin, 3-(N-suberoylargininyl)-arenobufagin, 3-(N-suberoylargininyl)-marinobufagin, and 3-(N-suberoylargininyl)-resibufogenin. The content of 20 batches of Bufonis Venenum was determined according to the Chinese Pharmacopoeia(2020 edition) by high-performance liquid chromatography, and the 2 batches of Bufonis Venenum, CS7(8.99% of total content) and CS9(5.03% of total content), with the largest difference in the total content of the three quality control indexes of the Chinese Pharmacopoeia(bufalin, cinobufagin, and resibufogenin) were selected to evaluate their anti-liver tumor activity based on the zebrafish model. The tumor inhibition rates of the 2 batches were 38.06% and 45.29%, respectively, proving that only using the quality control indexes of the Chinese Pharmacopoeia as the value orientation of Bufonis Venenum market circulation was unreasonable. This research provides data support for the effective utilization of Bufonis Venenum resources and the establishment of a rational quality evaluation system of Bufonis Venenum.
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- 2023
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26. [Clinical application value of Huanglian Jiedu Pills in improving syndrome of excess heat and fire toxin based on phase Ⅱ clinical trial study on plasma ATP, 4-HNE, and ACTH levels].
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Luo KK, Zhao HY, Si N, Bian BL, Xia W, Chen YQ, Wang MX, Wei XL, Li X, Qin GY, Yang J, Zhou YY, and Wang HJ
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- Humans, Medicine, Chinese Traditional, Adenosine Triphosphate, Adrenocorticotropic Hormone, Hot Temperature
- Abstract
A randomized, double-blind, placebo-controlled, multi-center phase Ⅱ clinical trial design was used in this study to recruit subjects who were in line with the syndrome of excess heat and fire toxin, and were diagnosed as recurrent oral ulcers, gingivitis, and acute pharyngitis. A total of 240 cases were included and randomly divided into a placebo group and a Huanglian Jiedu Pills group. The clinical efficacy of Huanglian Jiedu Pills in treating the syndrome of excess heat and fire toxin was evaluated by using the traditional Chinese medicine(TCM) syndrome scale. Enzyme-linked immunosorbent assay(ELISA) was used to determine and evaluate the levels of adenosine triphosphate(ATP), 4-hydroxynonenal(4-HNE), and adrenocorticotropic hormone(ACTH) in plasma of the two groups before and after administration and to predict their application value as clinical biomarkers. The results showed that the disappearance rate of main symptoms in the Huanglian Jiedu Pills group was 69.17%, and that in the placebo group was 50.83%. The comparison between the Huanglian Jiedu Pills group and the placebo group showed that 4-HNE before and after administration was statistically significant(P<0.05). The content of 4-HNE in the Huanglian Jiedu Pills group decreased significantly after administration(P<0.05), but that in the placebo group had no statistical significance and showed an upward trend. After administration, the content of ATP in both Huanglian Jiedu Pills group and placebo group decreased significantly(P<0.05), indicating that the energy metabolism disorder was significantly improved after administration of Huanglian Jiedu Pills and the body's self-healing ability also alleviated the increase in ATP level caused by the syndrome of excess heat and fire toxin to a certain extent. ACTH in both Huanglian Jiedu Pills group and placebo group decreased significantly after administration(P<0.05). It is concluded that Huanglian Jiedu Pills has a significant clinical effect, and can significantly improve the abnormal levels of ATP and 4-HNE in plasma caused by the syndrome of excess heat and fire toxin, which are speculated to be the effective clinical biomarkers for Huanglian Jiedu Pills to treat the syndrome of excess heat and fire toxin.
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- 2023
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27. [Determination and comparison of 10 active components in different parts of Loropetalum chinensis based on ultra-high performance liquid chromatography-tandem mass spectrometry].
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Zhou YY, Wei XL, Song JF, Si N, Zhang Y, Zhao HY, Li ML, Liu HY, Gao WY, Luo KK, Wang HJ, and Bian BL
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- Chromatography, High Pressure Liquid methods, Quinic Acid, Chromatography, Liquid, Tandem Mass Spectrometry methods, Drugs, Chinese Herbal chemistry
- Abstract
To establish a method for the simultaneous determination of ellagic acid, quercetin, gallic acid, kaempferol, myricetin, tiliroside, salidroside, isoquercetin, chlorogenic acid, and quinic acid in the leaves, flowers, fruits, and roots of Loropetalum chinensis by ultra-performance liquid chromatography-tandem mass spectrometry, and provide references for the development and utilization of L. chinensis resources. The analysis was performed on the chromatographic column ACQUITY UPLC HSS T3(2.1 mm×100 mm, 1.8 μm) with a gradient mobile phase of acetonitrile-0.2% formic solution at the flow rate of 0.3 mL·min~(-1). Column temperature was 30 ℃ and injection volume was 2 μL. Multiple reactive ion monitoring mode(MRM) was used in the negative ion ionization mode of electrospray ion source. The 10 active components had a good linear relationship, and the established method was stable, simple, and accurate. The 10 active components existed in different parts of L. chinensis, with significant different content. The main components in different parts of L. chinensis were polyphenols, with the highest content, followed by flavonoids. The content of 10 active components was generally high in flowers. Among them, the content of quinic acid was the highest, reaching 22.539 1 mg·g~(-1). This study elucidates the differences of active components in the same part and the different parts of L. chinensis, thereby providing basis for the research on the pharmacodynamic substances of L. chinensis and references for the comprehensive development and utilization of L. chinensis resources.
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- 2022
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28. [Biomarkers related to cognitive dysfunction in APP/PS1 mice based on non-targeted metabonomics and intervention mechanism of Huanglian Jiedu Decoction].
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Wang LN, Gu XR, Si N, Wang HJ, Zhou YY, Bian BL, and Zhao HY
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- Animals, Mice, Metabolomics methods, Biomarkers, Mice, Transgenic, Tyrosine, Drugs, Chinese Herbal pharmacology, Cognitive Dysfunction drug therapy
- Abstract
Through the non-targeted metabonomics study on endogenous substances in APP/PS1 transgenic mice, this paper aimed to discover biomarkers related to APP/PS1 mice with cognitive dysfunction, and find targets of Huanglian Jiedu Decoction(HLJDD) in the treatment of Alzheimer's disease(AD) and its mechanism. The brain tissue and serum metabolic mass spectrometry of mice were analyzed by ultra-high performance liquid chromatography-Orbitrap mass spectrometry(UPLC-Orbitrap MS). Through partial least squares-discriminant analysis(PLS-DA) and orthogonal partial least squares-discriminant analysis(OPLS-DA), the metabolic data of the normal group, the model group, the high-dose and low-dose HLJDD groups, and the berberine group were compared and analyzed to screen out potential biomarkers, and the relevant metabolic pathways were constructed with the help of the Kyoto Encyclopedia of Genes and Genomes(KEGG) database. Forty-five potential endogenous metabolites were identified, including 13 in brain and 35 in serum, among which leukotriene B4, tyrosine, and adenosine were expected to be differential metabolites related to cognitive function. HLJDD recalled 22 differential metabolites, and the pathways mainly involved in aminoacyl-tRNA biosynthesis, valine, leucine and isoleucine biosynthesis, pantothenic acid and coenzyme A biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, and arachidonic acid metabolism. These pathways suggested that the main mechanism of HLJDD in the intervention of AD was to inhibit central and peripheral inflammation, and regulate energy metabolism, fatty acid metabolism, and amino acid metabolism. HLJDD has a certain effect on the improvement of cognitive dysfunction, and regulates relative pathways by recalling endogenous differential metabolites, which helps to further discover the biomarkers of AD and clarify the intervention mechanism of HLJDD in the treatment of AD.
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- 2022
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29. [Determination of neohesperidin and naringin in Qingfei Paidu Granules by RP-HPLC and their transfer rates in preparation process].
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Zhang Y, Wang HJ, Yang LX, Zhou YY, Zhao HY, Li ML, Bian BL, Wu HK, Zhu HY, Si N, and Han L
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- Chromatography, High Pressure Liquid methods, Drugs, Chinese Herbal, Flavanones, Hesperidin analogs & derivatives
- Abstract
The present study established an RP-HPLC method for simultaneous determination of two active components in Qingfei Paidu Granules and investigated the transfer rates of neohesperidin and naringin in the preparation process to provide references for improving the quality control standard and production of Qingfei Paidu Granules.RP-HPLC was performed on a YMC Triart C_(18) column(4.6 mm×150 mm, 5 μm)with column temperature of 30 ℃, acetonitrile(A) and 0.2% phosphoric acid solution(B) as mobile phases for gradient elution at a flow rate of 1.0 mL·min~(-1) and detection wavelength of 284 nm.Good linearity was observed for naringin at 0.10-1.0 μg(R~2=0.999 9) and neohesperidin at 0.12-1.2 μg(R~2=0.999 9).The average recovery of naringin was 99.52% with an RSD of 1.2%, and that of neohesperidin was 100.8% with an RSD of 1.2%.The transfer rates of naringin and neohesperidin between medicinal materials, extracts, concentrates, and granules were measured by this method.The average transfer rate of naringin from medicinal materials to granules was 54.89%±4.38%, and that of neohesperidin was 57.63%±5.88%.The process from medicinal materials to extracts was presumedly the key link affecting the whole preparation process.The established method is simple and sensitive and can be adopted for the quality control of Qingfei Paidu Granules.Meanwhile, it can be used to investigate the transfer rate of neohesperidin and naringin in the preparation of Qingfei Paidu Granules, and further improve the quality control standard of Aurantii Fructus Immaturus in Qingfei Paidu Granules.
- Published
- 2022
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- View/download PDF
30. [Comprehensive evaluation system for quality of Chinese medicinal decoction pieces based on "experience-ingredients-activity-electronic sensing"].
- Author
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Li ML, Gao WY, Wang HJ, Si N, Zhou YY, Wei XL, Bian BL, and Zhao HY
- Subjects
- China, Electronics, Medicine, Chinese Traditional, Restraint, Physical, Drugs, Chinese Herbal
- Abstract
Quality evaluation of Chinese medicinal decoction pieces is vital for the development of the downstream industries, and is an important channel for implementing the strategy of "higher quality, higher price, and priority for the high quality" for traditional Chinese medicine. At the moment, the quality of Chinese medicinal decoction pieces is mainly evaluated based on chemical component examination. Considering the weak preliminary research foundation and poor research conditions, traditional experience-based evaluation is undervalued in the quality rating of Chinese medicinal decoction pieces. However, traditional experience is a summary of the quality of Chinese medicinal materials based on clinical experience, which thus can be a potential basis for the quality evaluation of the decoction pieces. It is a challenge in the evaluation of Chinese medicinal decoction pieces to objectify the traditional experience-based evaluation from multiple aspects such as chemistry, effect, and characterization via modern techniques. Therefore, this study developed the "experience-ingredients-activity-electronic sensing" evaluation system for Chinese medicinal decoction pieces on the basis of experience-based assessment, chemical ingredients that can truly reflect the traditional experience, biological effect assessment, and electronic sensory evaluation, which is expected to quantify the traditional experience of quality evaluation of Chinese medicinal decoction pieces via chemistry, biology, and sensory simulation. The evaluation system can serve as a reference for clinical experience-based quality evaluation of Chinese medicinal decoction pieces.
- Published
- 2022
- Full Text
- View/download PDF
31. [Identification of chemical constituents of Xiaoer Chiqiao Qingre Granules based on UHPLC-LTQ-Orbitrap-MS/MS and network pharmacology analysis].
- Author
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Zhang Y, Zhou YY, Gao WY, Cui MY, Bian BL, Ni LQ, Wang K, Wang HJ, Si N, and Zhao HY
- Subjects
- Child, Chromatography, High Pressure Liquid, Chromatography, Liquid, Humans, Network Pharmacology, Drugs, Chinese Herbal, Tandem Mass Spectrometry
- Abstract
This study aimed to qualitatively analyze the chemical components in Xiaoer Chiqiao Qingre Granules(XRCQ) by UHPLC-LTQ-Orbitrap-MS/MS and identify its material basis. The absorbed components in plasma were combined for exploring the potential action mechanism by integrated network pharmacology. ACQUITY UPLC HSS T3(2.1 mm×100 mm, 1.8 μm) column and mobile phase system of 0.1% formic acid solution(A)-acetonitrile(B) were used for gradient elution, followed by high resolution liquid chromatography-mass spectrometry in both positive and negative ion scanning modes. According to the precise relative molecular mass and MS/MS fragment ions, a total of 124 chemical components were identified in XRCQ by the comparison with references and literature reports, among which 29 compounds were completely confirmed by comparison with reference substances. Then, the main absorbed components of XRCQ in plasma were also analyzed and clarified by UHPLC-LTQ-Orbitrap-MS/MS. BATMAN-TCM and SwissTargetPrediction were used for target prediction of absorbed components in plasma. Following the plotting of association network with Cytoscape 3.8.2, the core targets were subjected to GO and KEGG pathway enrichment analysis and a component-target-pathway network was constructed. A total of eight main targets of XRCQ against fever in children were obtained together with eight absorbed components in plasma, including glycyrhydinic acid, hesperidin, emodin, reticuline, daidzein, magnolignan C, magnolignan A, and magnolaldehyde D. It was inferred that XRCQ might improve alimentary system abnormality, inflammatory response, oxidative stress, and endocrine disorder through tumor necrosis factor, PI3 K-AKT, and other signaling pathways. The present study comprehensively expounded the chemical profiles of XRCQ and the main absorbed components in plasma and predicted the potential mechanism of XRCQ based on integrated network pharmacology, which has provided certain theoretical reference for the clinical application of XRCQ.
- Published
- 2021
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32. [Study on anti-inflammatory activity and mechanism of indolealkylamines in toad skin on LPS-activated neutrophils].
- Author
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Zhang Y, Ding YH, Wei XL, Zhou YY, Si N, Yang J, Wang HJ, Zhao HY, and Bian BL
- Subjects
- Animals, Anti-Inflammatory Agents pharmacology, Apoptosis, Bufonidae, Skin, Lipopolysaccharides toxicity, Neutrophils
- Abstract
Indolealkylamines(IAAs) are the main hydrophilic substances in toad skin, mainly including free N-methyl-5-hydroxytryptamine, bufotenine, bufotenidine, dehydrobufotenine, and binding bufothionine. In this study, the LPS-activated neutrophils were used to investigate the structure-activity relationship and anti-inflammatory mechanism of the above-mentioned five monomers from the toad skin in vitro. The neutrophils were divided into the control group, model group(1 μg·mL~(-1) LPS), positive drug group(100 μg·mL~(-1) indometacin), as well as the low-(50 μg·mL~(-1)), medium-(100 μg·mL~(-1)) and high-dose(200 μg·mL~(-1)) free N-methyl-5-hydroxytryptamine, bufotenine, bufotenidine, dehydrobufotenine, and binding bufothionine groups. The levels of IL-6, TNF-α and IL-1β in the neutrophil supernatant of each group was measured by enzyme-linked immunosorbent assay(ELISA) after LPS stimulation, followed by the detection of apoptosis in each group after Annexin V/PI staining. The protein expression levels of caspase-3, Bax, Bcl-2, beclin1, LC3-I, and LC3-Ⅱ were assayed by Western blot. The results showed that IAAs reduced the excessive secretion of inflammatory cytokines caused by LPS compared with the model group. Besides, the activity of each free IAAs(N-methyl-5-hydroxytryptamine, bufotenine, bufotenidine and dehydrobufotenine), especially bufotenine, was stronger than that of the binding bufothionine. As revealed by Annexin V/PI staining, LPS delayed the early apoptosis of neutrophils compared with the control group, while bufotenine promoted the apoptosis of neutrophils in a dose-dependent manner, which might be related to the elevated expression of apoptosis-related protein Bax/Bcl-2. In addition, LPS activated the autophagy pathways in neutrophils. This study confirmed the efficacy of IAAs in reducing the secretion of inflammatory cytokines in neutrophils induced by LPS for the first time. For instance, bufotenine exerts the anti-inflammatory effect possibly by inducing the apoptosis of neutrophils.
- Published
- 2021
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33. [Identification and attribution of chemical constituents of Qingfei Paidu Decoction based on UHPLC-LTQ-Orbitrap-MS technology].
- Author
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Zhou YY, Gao WY, Gu XR, Chen ZQ, Zhao HY, Bian BL, Yang LX, Si N, Wang HJ, and Tan Y
- Subjects
- Chromatography, High Pressure Liquid, Coumarins analysis, Flavonoids analysis, Mass Spectrometry, Drugs, Chinese Herbal
- Abstract
UHPLC-LTQ-Orbitrap-MS was developed for the identification of chemical constituents in Qingfei Paidu Decoction, which will clarify its material basis. ACQUITY UHPLC HSS T3 chromatography column(2.1 mm×100 mm, 1.8 μm) was used with 0.1% formic acid(B)-acetonitrile(A) as the mobile phase in gradient elution. The decoction was detected by high-resolution liquid chromatography-mass spectrometry equipped with an ESI ion source in positive and negative mode. Based on the accurate mass measurements, retention time, mass fragmentation patterns combined with comparison of reference and literature reports, a total of 87 major compounds including 43 flavonoids, 9 alkaloids, 4 triterpenoid saponins, 1 sesquiterpene, 2 coumarins, 10 phenolic acids and 18 other compounds were tentatively screened and characterized. UHPLC-LTQ-Orbitrap-MS was employed to comprehensively elucidate the chemical components in Qingfei Paidu Decoction, which basically covered 20 Chinese medicines except gypsum in Qingfei Paidu Decoction. These collective results provide a scientific basis for further research on the quality control standard of Qingfei Paidu Decoction.
- Published
- 2020
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34. [Application of MALDI-mass spectrometry imaging in spatial distribution of secondary metabolites in medicinal plants -- a case study of Lepdium meyenii root].
- Author
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Mi YL, Sun W, Li ML, Zhao HY, Bian BL, and Zhou YY
- Subjects
- Chromatography, High Pressure Liquid, Plants, Medicinal chemistry, Secondary Metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Lepidium chemistry, Phytochemicals analysis, Plant Roots chemistry
- Abstract
Matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) is a novel technique for in-situ distribution of various substances in tissue without labeling. This technique is increasingly applied to the study of medicinal plants owing to its high spatial resolution and its potential of in-situ analysis in small molecules. In this study, the structural information and their fragmentation patterns of the midazole alkaloids(1,3-dibenzyl-4,5-dimethylimidazolium chloride and 1,3-dibenzyl-2,4,5-trimethylimi-dazolium chloride) and benzylglucosinolate in the medicinal plant Maca(Lepdium meyeni) root were analyzed by ultra-high-performance liquid phase combined with LTQ-Orbitrap mass spectrometry(UHPLC-HR-MS). The localization of these active ingredients in the cross-sections of Maca root was performed by MALDI-MSI. These results demonstrated that the two types of imidazole alkaloids had a similar distributed pattern. They were located more in the cortex and the periderm than those in the medulla of a lateral root, while the localization of benzylglucosinolate was concentrated in the center of the root rather than in the cortex and the periderm. The precise spatial distribution of various secondary metabolites in tissue provides an important scientific basis for the accumulation of medicinal plant active ingredients in tissues. In addition, this imaging method is a promising technique for the rapid evaluation and identification of the active ingredients of traditional Chinese medicine in plant tissues, as well as assisting the research on the processing of medicinal plants.
- Published
- 2020
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35. [Pharmacokinetics of active ingredients of Pudilan Xiaoyan Oral Liquid in rats and effect on liver drug-metabolizing enzymes].
- Author
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Zhou YY, Gu XR, Li T, Bian BL, Wang HJ, Si N, and Zhao HY
- Subjects
- Administration, Oral, Animals, Chromatography, High Pressure Liquid, Chromatography, Liquid, Liver, Rats, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization, Drugs, Chinese Herbal pharmacokinetics, Tandem Mass Spectrometry
- Abstract
This study is aimed to establish a method for the determination of baicalin,baicalin and purpurin in the plasma of rats after oral administration of Pudilan Xiaoyan Oral Liquid( PDL) by using liquid chromatography-mass spectrometry( LC-MS),analyze the pharmacokinetics of three components in rats,and investigate the effects of PDL on drug-metabolizing enzymes in rat liver. C18 column was used for liquid chromatography separation,with acetonitrile-water( containing 0. 2% formic acid) as the mobile phase for gradient elution. The mass spectrometry was detected by electrospray ion source( ESI) under multi-reaction monitoring mode( MRM),as well as positive and negative ion alternating mode. Plasma sample collection was performed by using an automatic blood collection meter for small animals. The pharmacokinetic parameters were calculated by Win Nonlin software. The total protein concentration of rat liver microsomes and the total enzyme content of CYP450 were determined by BCA method and spectrophotometry respectively. The methodological study in terms of linear range,recovery rate,precision and sample stability,was used to confirm that the LC-MS analysis method established in this experiment was simple,exclusive,accurate and reliable,and can meet the requirement of determining the content of baicalin,oroxindin and corynoline in plasma after PDL administration in rats. The drug-time curve showed that baicalin and oroxindin had a bimodal phenomenon,and the pharmacokinetic parameters indicated that baicalin,oroxindin and corynoline in PDL had certain drug-like properties. After 7 consecutive days of PDL administration,the rat liver coefficient,total liver microparticle protein and CYP450 enzyme content were increased,but there was no significant difference,indicating that PDL was less likely to develop drug-drug interaction based on CYP enzyme. The results of this experiment can provide reference for the research on in vivo efficacy and drug interaction of PDL as well as on its clinical application.
- Published
- 2019
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36. [Identification and attribution of chemical compounds of Pudilan Antiphlogistic Oral Liquid].
- Author
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Zhao LJ, Gao WY, Gu XR, Wang HJ, Zhao HY, and Bian BL
- Subjects
- Chromatography, High Pressure Liquid, Tandem Mass Spectrometry, Anti-Inflammatory Agents analysis, Drugs, Chinese Herbal chemistry, Phytochemicals analysis
- Abstract
UPLC-ESI-Orbitrap-MS/MS was used to analyze,identify and attribute the chemical constituents in Pudilan Antiphlogistic Oral Liquid. The analysis was performed on an Agilent Eclipse XDB-C18(4.6 mm × 150 mm,3.5 μm) with a gradient mobile phase of methanol-0.1% formic solution system at the flow rate of 0.5 m L·min-1. The sample volume was 2 μL. The column temperature was30 ℃. The high-resolution orbitrap mass spectrometry was used as detector,with electrospray ion source in both positive and negative models,and the MS scanning ranged between m/z 50 and 2 000. Based on the analysis of mass spectrometry and literature reports,79 compounds were confirmed,including 30 alkaloids,28 organic acids,18 flavonoids and 3 coumarins. Finally,39 compounds,such as rutin,esculetin,gallic acid,caffeic acid,cichoric acid,were identified from Taraxacum mongolicum; 11 compounds,such as baicalin,baicalein,apigenin,chrysin,oroxylin A,were identified from Scutellaria baicalensis; 13 compounds,such as arginine,proline,hypoxanthine,epigoitrin,indirubin,were identified from Isatis indigotica; and 18 compounds,such as dehydrocheilanthifoline,oxysanguinarine,corynoline,protopine,spallidamine,were identified from Corydalis bungeana. After the analysis of chemical model and attribution,the contents of some compounds were high in Pudilan Antiphlogistic Oral Liquid,such as baicalin,wogonoside,baicalein,wogonin,apigenin,chrysin,skullcapflavonⅡ,oroxylin A,cichoric acid,chlorogenic acid,caffeic acid,esculetin,dehydrocheilanthifoline,dihydrosanguinarine,protopine,corynoline and indirubin. The established method is simple,accurate,rapid,sensitive and reproducible,and thus suitable for the qualitative identification and quantitative determination of Pudilan Antiphlogistic Oral Liquid,which lays a foundation for the systematic quality control and the establishment of whole-course traceability system of active ingredients.
- Published
- 2019
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37. [Lipid metabolism study of sodium norcantharidate in LO2 hepatocytes based on lipidomics].
- Author
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Zhao LJ, Si N, Gao B, Wei XL, Wang YL, Zhao HY, and Bian BL
- Subjects
- Cells, Cultured, Hepatocytes metabolism, Humans, Lipids analysis, Tandem Mass Spectrometry, Bridged Bicyclo Compounds, Heterocyclic pharmacology, Hepatocytes drug effects, Lipid Metabolism
- Abstract
In order to find the endogenous potential biomarkers of in vitro hepatic injury caused by NCTD-Na and elucidate the mechanism of hepatic injury of NCTD-Na,ultra-high performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) was used for lipidomics detection.Multivariate statistical analysis was used to study the endogenous lipid metabolic changes of human normal liver cells LO2 injury after the treatment with sodium norcantharidate(NCTD-Na).The results showed that the half maximal inhibitory concentration(IC50) of NCTD-Na was 0.034 mmol·L-1.A total of 280 differential metabolites were found between the control group and the low-dose group,with VIP > 2.0 and P<0.05.At the same time,a total of 273 differential metabolites were found between the control group and the high-dose group,with VIP > 2.0 and P<0.05.Cell metabolite profiles showed clear separation among control group,the low-dose group and the high-dose group,and 111 differential metabolites were found,with VIP > 2.0,P<0.05,RSD<30% and in a dose-dependent manner.It was found that most of the above differential metabolites were lipid metabolites after the analysis of simple preparnation methods and database search.A total of 32 potential biomarkers were identified,including 3 phosphatidylcholine(PC),5 lysophosphatidylcholine(Lyso PC),3 ceramide(Cer),1 sphingomyelin(SM),1 phosphatidylethanolamine(PE),10 lysophosphatidylethanolamine(LysoPE),4 diacylglycerol(DG),1 Phosphatidic acid(PA),1 lysophosphatidic acid(Lyso PA),1 phosphatidyl glycerol(PG),1 fatty acid hydroxy fatty acid(FAHFA) and 1 phosphatidylserine(PS).The changes of PCs,Cers,SM,PE and DGs were closely related liver protection,DNA methylation and self-repair in hepatocytes,apoptosis,methylation and detoxification of carcinogens,as well as lipid peroxides production process.Also,they had impact on the proliferation of hepatocytes,differentiation and gene transcription disorders.Cells stimulated by NCTD-Na could promote the production of PA as well as the synthesis and catabolism of FAHFA in a variety of ways.The levels of Lyso PCs,LysoPEs and Lyso PA were correlated with PCs,PE and PA;PE and PS might have valgus during apoptosis,triggering phagocytosis.
- Published
- 2019
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38. [Pharmacodynamics of Huanglian Jiedu decoction in Alzheimer's disease (AD) model rats and effect on improvement of inflammation microenvironment in brain].
- Author
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Gu XR, Fang SY, Ren W, Wang HJ, Yang J, Si N, Bian BL, and Zhao HY
- Subjects
- Animals, Brain, Inflammation, Rats, Rats, Sprague-Dawley, Alzheimer Disease, Drugs, Chinese Herbal
- Abstract
To study the pharmacokinetics of active ingredients (alkaloids, iridoids and flavonoids) in Huanglian Jiedu decoction (HLJDD) in Alzheimer's disease (AD) model rats, and investigate its mechanism in treatment of AD. All of rats were divided into normal control group ( n =6), shame operation group ( n =6) and model group ( n =12). Rats in shame operation group received daily subcutaneous injection of D-galactose (D-gal 50 mg·kg⁻¹) for a total of 45 d to induce subacute aging model. Based on the operation of shame operation group, the rats in model group were given with an injection of Aβ₂₅₋₃₅ (4.0 g·L⁻¹)-ibotenic acid (2.0 g·L⁻¹) into the nucleus basalis magnocellularis. Then rats in model group were divided into HLJDD one day administration group ( n =6) and HLJDD one week group ( n =6). The plasma concentration of alkaloids, iridoid and flavonoids was determined by liquid chromatography tandem mass spectrometry (LC-QQQ-MS) at different time points. The levels of seven inflammatory factors (MIP-2, IL-1β, IL-2, IL-8, IL-10, IL-13, TNF-α) in cerebrospinalfluid (CSF) were measured by Bio-Plex multi-factor detection technology. Iridoids in AD model rats, with high bioavailability, were easily absorbed and eliminated. The plasma concentration of alkaloid was lower, and the AUC (area under the curve) was higher in HLJDD one week group than that in HLJDD one day group. The plasma concentration-time curves of flavonoids showed obvious bimodal phenomena. After the gastric administration of HLJDD, the inflammatory factors in CSF of AD rats demonstrated a callback trend, including IL-1β/IL-10 ( P <0.05) with significant difference. The pharmacokinetic behaviors of iridoids, alkaloids and flavonoids (41 compounds) in AD model rats were fundamentally elucidated, and HLJDD can improve the central inflammatory status of AD rats by regulating the levels of inflammatory factors., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2018
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39. [Chemical studieson hydrophilic constituents of toad skin].
- Author
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Xin SK, Si N, Wang HJ, Yang J, Gao B, Wei XL, Zhao HY, and Bian BL
- Subjects
- Animals, Chromatography, High Pressure Liquid, Bufonidae, Skin chemistry
- Abstract
Fifteen compounds were isolated from the toad skin by a combination of various chromatographic methods including macroporous resin, silica gel, ODS and semi-preparative HPLC. Their structures were identified as 4,5-dimethyl-1,3,4,5-tetrahydropyrrolo[4,3,2-de]quinolin-6-ol(1), serotonin(2), N-methyl serotonin(3), O-methyl bufotenine(4), 1,2,3,4-tetrahydro-6-hydroxy-β-carboline(5), O-methylserotonin(6), glycinebetaine(7), caffeine(8), bufotenine(9), shepherdine(10), tryptophan(11), (5-hydroxy-1H-indol-3-yl)acetic acid(12), 5-hydroxy tryptophol(13), 2-methyl-6-hydroxy-1,2,3,4-tetrahydro-β-carboline(14), bufothionine(15). Among them, compound 1 was a new compound,compound 5 was a new natural product. Compounds 4-8 and 10-14 were separated from toad skin for the first time., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2016
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40. [Study on characteristic spectrum of ingredients from different species Cordyceps and its anti-fibrotic activity on human embryonic fibroblasts].
- Author
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Si N, Peng B, Zhao HY, Wang HJ, Yang J, Li JR, Sun W, and Bian BL
- Subjects
- Adenosine pharmacology, Cells, Cultured, Deoxyadenosines pharmacology, Fibrosis, Humans, Transforming Growth Factor beta1, Uridine pharmacology, Cordyceps chemistry, Fibroblasts drug effects
- Abstract
In this study, 10 samples of parasites, cursive, and the whole from six different species of Cordyceps were determined and compared by HPLC and LC-MS methods. Uridine, adenosine, and cordycepin were selected as the main evaluation index. The anti-fibrotic activity of different species Cordyceps extracts was observed using in vitro TGF-β1-induced ECM accumulation in human embryonic fibroblasts CCC-ESF-1. The results demonstrated that the number of atoms and hyphae ingredients of different species showed little difference, however, the content distribution of each component has obvious significance. The in vitro anti-fibrotic activities of different species were as follow: Cordyceps flower > Cicada Cordyceps (Cicada flower)> Silkworm Cordyceps> Tussah Cordyceps>natural Cordyceps. Our preliminary data could serve as reference for the discovery of artificial alternatives of natural Cordyceps., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2016
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41. [Study of screening pseudoallergenic substances of Shuanghuanglian injection].
- Author
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Yi Y, Zhang YS, Li CY, Zhao HY, Xiao HB, Li GQ, Lu YT, Han JY, Zhao Y, Wang HJ, Si N, Liang AH, and Bian BL
- Subjects
- Animals, Drugs, Chinese Herbal analysis, Furans adverse effects, Glucosides adverse effects, Glycosides adverse effects, Injections, Male, Mice, Mice, Inbred ICR, Drug Hypersensitivity etiology, Drugs, Chinese Herbal adverse effects
- Abstract
In this study, chemistry, biology and pharmacology were combinated to screen pseudoallergenic substances of Shuang-huanglian injection (SHLI) so that to establish a scientific and systematic approach to screen pseudoallergenic substances of traditional Chinese medicine injections. The mouse pseudoallergic reaction models were used to screen the pseudoallergic reaction of SHLI's intermediate extract and the intermediate extract's component or ingredient. Among the three intermediates of Shuanghuanglian injection (extract of Scutellaria baicalensis, extract of Lonicera japonica, extract of Forsythia suspensa) , pseudoallergic action of Forsythia suspensa was the strongest, Forsythia suspesnsa's pseudoallergic reaction mainly associated with the composition with largerchemical polarity. Further it was found that forsythiaside A and arctiin which existed in the the composition with largerchemical polarity caused obvious pseudoallergic reactions. SHLI with removal forsythoside A with the technology of HPLC-MS displayed reduced pseudoallergic reaction and a significant improved safety. This study provided a scientific basis for SHLI process improvements and also offered idea and research foundation for screening pseudoallergenic substances injections in other TCM injections.
- Published
- 2015
42. [Chemical constituents of bufadienolides in cinobufacino injection].
- Author
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Han LY, Si N, Liu JQ, Zhao HY, Yang J, Bian BL, and Wang HJ
- Subjects
- Animals, Bufanolides chemistry, Injections, Bufanolides isolation & purification, Bufo bufo, Skin chemistry
- Abstract
Cinobufacino injection is purified from water extraction of the skin of Bufo bufo gargarizans, which has been widely used for various cancers in clinic with significant anti-tumor effects. Bufadienolides were regarded as the main active constituents of cinobufacino injection in previous reports. In present study, 6 bufadienolides were isolated and purified from Cinobufacino injection. Their structures were identified as 3-epi-ψ-bufarenogin (1), ψ-bufarenogin (2), 3-epi-arenobufagin (3), arenobufagin (4), 3-epi-gamabufotalin (5), and 3-oxo-arenobufagin (6), separately. Among them, 1 and 3 were new compounds, 5 and 6 were new natural products. Compounds 1, 2 and compounds 3, 4 were two pairs configuration isomers at C-3, separately.
- Published
- 2014
43. [Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS].
- Author
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He J, Li Y, Si N, Zhao HY, Bian BL, and Wang HJ
- Subjects
- Animals, Chromatography, High Pressure Liquid, Tandem Mass Spectrometry, Amphibian Venoms chemistry, Bufanolides chemistry, Bufonidae
- Abstract
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
- Published
- 2014
44. [In vivo comparison analysis of scandoside methyl ester metabolites in four kinds of liver microsomes using ultra-performance liquid chromatography combined with electrospray ionization tandem orbitrap mass spectrometry].
- Author
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Li Y, Wang HJ, He J, Si N, Yang J, Zhao HY, and Bian BL
- Subjects
- Animals, Chromatography, High Pressure Liquid, Dogs, Esters metabolism, Humans, Macaca mulatta, Rats, Spectrometry, Mass, Electrospray Ionization, Iridoids metabolism, Microsomes, Liver metabolism
- Abstract
In order to clarify the metabolism pathways of scandoside methyl ester, the analysis of metabolites profiling in four kinds of liver microsomes was performed by using an ultra-performance liquid chromatography/ electrospray-tandem mass spectrometry (UPLC-ESI-MS). The data obtained from the 0 h-incubation and the 2 h-incubation were compared and analyzed. After incubation, 5 metabolites of scandoside methyl ester were found in rat, Beagles, rhesus monkey and human liver microsome. The results showed that scandoside methyl ester's major metabolic pathway in the liver microsomes is hydrolysis, oxidation and reduction reactions, and there are certain kinds differences between species. The study provides a research base for further research about iridoid compounds in vivo metabolic pathways.
- Published
- 2014
45. [Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method].
- Author
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Liu JQ, Si N, Yang J, Zhao HY, Bian BL, and Wang HJ
- Subjects
- Animals, Bufanolides chemistry, Chromatography, High Pressure Liquid, Molecular Structure, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Amphibian Venoms chemistry, Bufanolides analysis, Bufo bufo
- Abstract
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
- Published
- 2014
46. [LC-FT-ICR-MS analysis of the prototypes and metabolites in rat plasma after administration of huang-lian-jie-du decoction].
- Author
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Zuo R, Wang HJ, Si N, Zhao HY, Yang J, and Bian BL
- Subjects
- Administration, Oral, Animals, Biotransformation, Chromatography, High Pressure Liquid, Drug Combinations, Drugs, Chinese Herbal administration & dosage, Drugs, Chinese Herbal isolation & purification, Male, Plants, Medicinal chemistry, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Alkaloids blood, Drugs, Chinese Herbal metabolism, Flavonoids blood, Iridoids blood
- Abstract
To obtain chemical constituent information of rat plasma after oral administration of Huang-Lian-Jie-Du Decoction (HLJDD), a LC-FT-ICR-MS method has been established, and both positive and negative ions scan modes were include in the analysis. By comparing their retention time, high resolution mass data of HLJDD extracts, blank plasma and dosed plasma, 38 constituents, including 22 prototype compounds and 16 metabolites, were detected in rat plasma after oral administration of HLJDD. In the 22 prototype compounds, 16 constituents were determined unambiguously by comparing with references. In the analysis of metabolites, phase II reactions like glucuronidation and sulfation were the major biotransformation pathways of HLJDD. M11 was observed as the only phase I metabolite in present experiment. The results will be beneficial for the further pharmacokinetics and pharmacological evaluations of HLJDD.
- Published
- 2014
47. [In vitro anti-proliferation effect of peptides from cinobufacini injection].
- Author
-
Wu X, Gao B, Yang J, Bian BL, and Wang HJ
- Subjects
- Amphibian Venoms administration & dosage, Amphibian Venoms isolation & purification, Animals, Antineoplastic Agents isolation & purification, Bufonidae, Cell Line, Tumor, HT29 Cells, Humans, Injections, Medicine, Chinese Traditional, Peptides isolation & purification, Skin chemistry, Amphibian Venoms chemistry, Antineoplastic Agents pharmacology, Cell Proliferation drug effects, Peptides pharmacology
- Abstract
Cinobufacini is an aqueous extract of Bufo bufo gargarizans Cantor dried skin, which has been widely used for cancer therapy in China. So far, its active components are still not very clear. In previous reports, bufadienolides with low-concentration were usually studied because of their anticancer effects. However, the high polarity constituents in cinobufacini are less investigated. The present study found that more than 50% contents of cinobufacini were water-soluble peptides. Then, in vitro anticancer experiments were carried out, including human stomach cancer cell lines BGC823 and MCG803, human colon cancer cell lines DLD-1 and HT-29, and human pancreatic cancer cell line MIAPACA-2. The IC50 for these cell lines model were ranged from 25-123 microgmL(-1). The results indicated that these peptides showed similar activity with cinobufacini injection. As a conclusion, this study provides a new and further understanding of anticancer components in cinobufacini injection.
- Published
- 2012
48. [Studies on chemical constituents and volatile oil of Xiaochengqi decoction].
- Author
-
Fan MX, Wang HJ, Li XM, Li PY, and Bian BL
- Subjects
- Anthraquinones chemistry, Biphenyl Compounds chemistry, Cinnamates chemistry, Cyclohexenes chemistry, Cymenes, Emodin analogs & derivatives, Emodin chemistry, Gallic Acid chemistry, Gas Chromatography-Mass Spectrometry, Lignans chemistry, Limonene, Monoterpenes chemistry, Sitosterols chemistry, Terpenes chemistry, Drugs, Chinese Herbal chemistry, Oils, Volatile chemistry
- Abstract
Objective: To study the chemical constituents and volatile oil of Xiaochengqi decoction., Method: The constituents in decoction were separated by means of column chromatography and their structures were identified by spectral data and compared with literature data. As well as the volatile oil of Xiaochengqi Fang were analyzed and identified by GC-MS., Result: Eleven compounds were isolated and identified as chrysophanol (1), physcion (2), magnolol (3), beta-sitosterol (4), sitosterol trans-cinnamic acid (5), emodin (6), aloe emodin (7), rhein (8), gallic acid (9), chrysophanol-8-O-beta-D-glucopyranoside (10), aurantiamarin (11). The volatile oils extracted with steam distillation from Xiaochengqi were identified 67 components, and the main components are including., Conclusion: All of the eleven compounds were isolated from Xiaochengqi decoction for the first time and the study on their activities in Chinese prescription is being carried out. D-limonene (42.61%), p-cymene (16.43%), and 8-terpinene (14.46%).
- Published
- 2008
49. [Study on preparation technology of plumula nelumbinis Alkaloid Dripping Pill and determination of content by HPLC].
- Author
-
Liu W, Wang HJ, Si N, and Bian BL
- Subjects
- Alkaloids chemistry, Benzylisoquinolines analysis, Benzylisoquinolines chemistry, Isoquinolines analysis, Isoquinolines chemistry, Microspheres, Particle Size, Phenols analysis, Phenols chemistry, Plants, Medicinal chemistry, Quality Control, Seeds chemistry, Solubility, Alkaloids analysis, Chromatography, High Pressure Liquid methods, Nelumbo chemistry, Polyethylene Glycols chemistry, Technology, Pharmaceutical methods
- Abstract
Objective: To determine the optimum technical parameters through controlling the different factors and the content of Dripping Pill., Method: Time of dissolution, spherical degree and pill weight as parameters, the influential factors were investigated by orthogonal test and then to determine the content of liensinine, isoliensinine, neferine in plumula nelumbinis Alkaloid Dripping Pill., Result: The optimum condition: 75% PEG4000 as matrix, methyl-silicon oil as refrigerant, 4 cm dripping distance, 10 degrees C refrigerant, 30 drippings per minute., Conclusion: The good quality pills can be produced through this process and the HPLC determination method is simple, reliable and can be used in the quality control of plumula nelumbinis Alkaloid Dripping Pill.
- Published
- 2007
50. [Solubilities of ephedrine and d-pseudo-ephedrine from different sizes of ultramicro-particles of the compound traditional Chinese herbs Maxing Shigan decoction].
- Author
-
Si N, Yang J, and Bian BL
- Subjects
- Calcium Sulfate chemistry, Drug Combinations, Drugs, Chinese Herbal administration & dosage, Drugs, Chinese Herbal isolation & purification, Ephedra chemistry, Ephedrine chemistry, Glycyrrhiza uralensis chemistry, Particle Size, Powders, Prunus chemistry, Solubility, Drugs, Chinese Herbal chemistry, Ephedrine analysis, Plants, Medicinal chemistry, Technology, Pharmaceutical methods
- Abstract
Objective: To compare the solubilities of ephedrine and d-pseudo-ephdrine in the compound of traditional Chinese drugs--Maxing Shigan decoction with and without ultramicro-pulverizations using a RP-HPLC procedure., Method: The analytical column was a YMC ODS-C18(4.6 mm x 150 mm,4 microm). A mixture of water-acetonitrile-sodium dodecyl sulfate-phosphorous acid (650:350:5:1) was used as the mobile phase at flow rate of 1.5 mL x min(-1). The column temperature was 50 degrees C and the wavelength was 210 nm., Result: In this way, ephedrine and d-pseudo-ephedrine of the compound samples can be separated well and showed significant differences before and after the ultramicro-pulverizations., Conclusion: For the Maxing Shigan decoction, the dissolution rates and solubilites of ephedrine and d-pseudo-ephedrine ultramicro-pulverization were greatly improved.
- Published
- 2006
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