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C8H9NO3S, triclinic, P1̅ (no. 2), a = 6.984(4) Å, b = 7.494(4) Å, c = 9.174(5) Å, α = 97.249(9)°, β = 95.155(8)°, γ = 108.515(8)°, V = 447.4(4) Å3, Z = 2, Rgt(F) = 0.0538, wRref(F2) = 0.1617, T = 296(2) K.

C15H19N3O3S, triclinic, P1̅ (no. 2), a = 8.682(8) Å, b = 9.700(8) Å, c = 11.273(10) Å, α = 90.681(14)°, β = 112.624(13)°, γ = 112.632(13)°, V = 794.5(12) Å3, Z = 2, Rgt(F) = 0.0405, wRref(F2) = 0.1171, T = 296(2) K.

A simple thiocarbazone‐appended coumarin derivative 1 with high selectivity as a ratiometric fluorescence sensor towards Cu2+ and Zn2+ in a semi‐aqueous solution was synthesised and characterised. Upon the addition of Cu2+ and Zn2+, the emission of 1 solution undergoes a blue and red shift to 480 and 545 nm according to the intense blue and yellow colour under a 365‐nm ultraviolet lamp, respectively. Binding was confirmed by ultraviolet‐visible absorption analysis, fluorescence measurement, mass spectrometry, proton nuclear magnetic resonance, Fourier Transform‐infrared spectroscopy and density functional theory calculation. The results show that 1 coordinated with Cu2+ and Zn2+ in different modes, resulting in different ratiometric fluorescence responses. The detection limits of the sensor 1 for Cu2+ and Zn2+ were 100.8 and 11.5 nM, respectively. The association constants (Ka) of Cu2+ and Zn2+ with 1 were determined to be 5.59 × 104 and 5.08 × 104 M−1, respectively. Fluorescent microscopy experiments demonstrated that probe 1 has the potential to be used to monitor Zn2+ and Cu2+ in living cells. [ABSTRACT FROM AUTHOR]