1. Evidence for ARGONAUTE4–DNA interactions in RNA-directed DNA methylation in plants
- Author
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Lahmy, Sylvie, Pontier, Dominique, Bies-Etheve, Natacha, Laudié, Michèle, Feng, Suhua, Jobet, Edouard, Hale, Christopher J, Cooke, Richard, Hakimi, Mohamed-Ali, Angelov, Dimitar, Jacobsen, Steven E, and Lagrange, Thierry
- Subjects
Genetics ,Generic health relevance ,Amino Acid Motifs ,Arabidopsis Proteins ,Argonaute Proteins ,Chromatin ,DNA Methylation ,DNA ,Plant ,DNA-Directed RNA Polymerases ,Gene Silencing ,Models ,Biological ,RNA Interference ,RNA ,Plant ,Transcription ,Genetic ,Argonaute ,DNA methylation ,RdDM ,Biological Sciences ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Developmental Biology - Abstract
RNA polymerase V (Pol V) long noncoding RNAs (lncRNAs) have been proposed to guide ARGONAUTE4 (AGO4) to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we provide evidence, based on laser UV-assisted zero-length cross-linking, for functionally relevant AGO4-DNA interaction at RdDM targets. We further demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. We propose a model in which AGO4-DNA interaction could be responsible for the unique specificities of RdDM.
- Published
- 2016