Search

Accurate quantification of evapotranspiration (ET) at the watershed-scale remains an important research challenge. ET products from model simulations and remote sensing, even after incorporating in situ ET observations from flux towers in calibration or assimilation procedures, often produce different watershed areal-averaged ET estimates. These differences in ET estimates are magnified when they are integrated over time as part of water balance calculations. To address this challenge, we present a methodology for comparing watershed-average ET within a water balance framework that makes use of Gravity Recovery and Climate Experiment (GRACE)-observed terrestrial water storage change (TWSC). The methodology is demonstrated for South Carolina for a five-year period (2003-2007) using four different ET products: ET generated using a locally calibrated VIC model, a MODIS-derived ET product, and ET generated from two models (NOAH and VIC) as part of the North American Land Data Assimilation Systems 2 (NLDAS-2) project. The results of the example application suggest that the NLDAS-NOAH ET product is most consistent with GRACE-observed TWSC for the overall study region and time period. However, for periods of decreasing TWS, when ET becomes a more significant term in the water balance, the locally calibrated VIC model showed the most agreement with GRACE-observed TWSC. Application of the methodology for other regions and time periods can provide insight into different ET products when used for watershed-scale water resources management.

Most antimicrobial peptides (AMPs) induce membrane damage such as pore formation in bacterial cells, resulting in rapid cell death. On the other hand, bacterial cells have a large intracellular turgor pressure, i.e., an osmotic pressure (Π) due to higher osmolarity inside bacterial cells, but the effects of Π on the membrane permeation of the internal contents of lipid vesicles and cells through AMP-induced pores are unknown. Here, we investigated the effect of Π on the membrane permeability of a water-soluble fluorescent probe, AlexaFluor 488 hydrazide (AF488), when passing through peptidyl-glycylleucine-carboxyamide (PGLa)- or magainin 2 (Mag)-induced nanopores in giant unilamellar vesicles (GUVs). For the interaction of PGLa with single GUVs under Π, the onset of pore formation was followed by a gradual increase in the membrane permeability coefficient, M P , until M P reached a steady value, P s . On the other hand, for the interaction of Mag with single GUVs under Π, the onset of pore formation was rapidly followed by a change of M P to P s . Small Π values enhanced the P s values of AF488 passing through the PGLa- or Mag-induced nanopores. The mechanisms underlying the increase of P s at small Π values were discussed. Based on these results and our previous results that the membrane tension (due to Π) enhances rate of AMP-induced pore formation, we consider the role of turgor pressure in AMP-induced damage in bacterial membranes and the efflux of internal contents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

A significant turning point in the search for environmentally friendly energy storage options is the switch from lithium-ion to sodium-ion batteries. This review highlights the potential of sodium-ion battery (NIB) technology to address the environmental and financial issues related to lithium-ion systems by thoroughly examining recent developments in NIB technology. It is noted that sodium is more abundant and less expensive than lithium, NIBs have several benefits that could drastically lower the total cost of energy storage systems. In addition, this study examines new findings in important fields including electrolyte compositions, electrode materials, and battery performances of lithium-ion batteries (LIBs) and NIBs. The article highlights advancements in anode and cathode materials, with a focus on improving energy density, cycle stability, and rate capability of both LIBs and NIBs. The review also covers the advances made in comprehending the electrochemical mechanisms and special difficulties associated with NIBs, such as material degradation and sodium ion diffusion. Future research directions are discussed, with an emphasis on enhancing the scalability and commercial viability of sodium-ion technology over lithium on Electric Grid. Considering sustainability objectives and the integration of renewable energy sources, the review's assessment of sodium-ion batteries' possible effects on the future state of energy storage is included in its conclusion., (© 2024 The Chemical Society of Japan and Wiley-VCH GmbH.)

Time-restricted feeding (TRF) is a popular dietary strategy whereby daily food intake is limited to a <12h window. As little is known about the effects of TRF on cognitive and behavioral measures, the present study examined the effects of time-restricted (8h/day; zeitgeber time [ZT]12-20) or continuous access to a high-fat, high-sugar cafeteria-style diet (Caf; Caf and Caf-TRF groups; n=12 adult male Sprague-Dawley rats) or standard chow (Chow and Chow-TRF groups) on short-term memory, anxiety-like behavior, adiposity and gut microbiota composition over 13-weeks with daily food intake measures. TRF significantly reduced daily energy intake in Caf- but not chow-fed groups. In Caf-fed groups, TRF reduced the proportion of energy derived from sugar while increasing that derived from protein. Caf diet significantly increased weight gain, adiposity and fasting glucose within 4 weeks; TRF partially reduced these effects. Caf diet increased anxiety-like behavior in the Elevated Plus Maze in week 3 but not week 12, and impaired hippocampal-dependent place recognition memory in week 11; neither measure was affected by TRF. Global microbiota composition differed markedly between chow and Caf groups, with a small effect of TRF in rats fed chow. In both chow and Caf diet groups, TRF reduced microbiota alpha diversity measures of Shannon diversity and evenness relative to continuous access. Results indicate only limited benefits of TRF access to an obesogenic diet under these conditions, suggesting that more severe time restriction may be required to offset adverse metabolic and cognitive effects when using highly palatable diets., Competing Interests: Declarations of interest None., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Parkinson's disease (PD) is a progressive neurological disease that causes both motor and nonmotor symptoms. While our understanding of putative mechanisms has advanced significantly, it remains challenging to verify biomarkers with sufficient evidence for regular clinical use. Clinical symptoms are the primary basis for diagnosing the disease, which can be mild in the early stages and overlap with other neurological disorders. As a result, clinical testing and medical records are mostly relied upon for diagnosis, posing substantial challenges during both the initial diagnosis and the continuous disease monitoring. Recent biochemical, neuroimaging, and genetic biomarkers have helped us understand the pathophysiology of Parkinson's disease. This comprehensive study focuses on these biomarkers, which were chosen based on their relevance, methodological excellence, and contribution to the field. Biochemical biomarkers, including α-synuclein and glial fibrillary acidic protein (GFAP), can predict disease severity and progression. The dopaminergic system is widely used as a neuroimaging biomarker to diagnose PD. Numerous genes and genome wide association study (GWAS) sites have been related to the development of PD. Recent research on the SNCA gene and leucine-rich repeat protein kinase 2 (LRRK2) has shown promising results. By evaluating current studies, this review intends to uncover gaps in biomarker validation and use, while also highlighting promising improvements. It emphasizes the need for dependable and reproducible indicators in improving PD diagnosis and prognosis. These biomarkers may open up new avenues for early diagnosis, disease progression tracking, and the development of personalized treatment programs.

Risk stratification is an important initial step for primary prevention of atherosclerotic cardiovascular diseases. There are a number of scoring systems for this purpose worldwide. We tried to evaluate two most updated scoring systems. To assess which one is the better for Bangladeshi population residing in Bangladesh. This cross-sectional study was conducted in a secondary and a tertiary care hospital in Bangladesh from January 2019 to June 2019. Total 274 patients were included in the study. They were evaluated using ASCVD scoring system and QRISK3 scoring system for the risk of atherosclerotic cardiovascular event (myocardial infarction and/or stroke) in next 10 years. Average age of the patients was 57.1±12.8 years and 192 of them were male and 92 of them were female. Half (50.4%) of the patients were smoker, half (51.1%) of them were hypertensive, 45.6% of them were diabetic, 29.6% of them had family history of premature atherosclerotic cardiovascular diseases and 27.0% of them were overweight or obese. According to ASCVD scoring 36.5% patients were at high risk, 32.5% at intermediate risk, 16.4% at low risk of cardiovascular events in next 10 years and risk evaluation was not possible in 14.6% patients. According to QRISK3 scoring method 55.5% are at high risk, 20.8% at intermediate risk, 16.0% at low risk of cardiovascular events and evaluation was not possible in 7.7% patients. Predictive value of QRISK3 scoring system is better to detect more patients who are at high risk for atherosclerotic cardiovascular events in next 10 years. QRISK3 can also evaluate the patients at a younger age. At present QRISK3 is better system to evaluate cardiovascular risk in Bangladeshi population. We need further study to evaluate its role in the form of clinical efficacy and cost effectiveness.

This review focuses on the deformation and poration of lipid vesicles caused by the interaction of anionic magnetite nanoparticles (MNPs). Effects of various factors, such as surface charge density, salt and sugar concentrations in buffer, membrane cholesterol content, polymer-grafted phospholipid, and membrane potential have been discussed for the interaction of MNPs with lipid vesicles. To quantify these effects on the vesicles, compactness, fraction of deformation and poration, dynamics of membrane permeation, and kinetics of membrane permeation have been critically evaluated. The review explores the potential advancements as well as future directions of the research field in the biomedical application of MNPs., Competing Interests: The authors affirm that there are no conflicts of interest., (This journal is © The Royal Society of Chemistry.)

Irreversible electroporation induces permanent permeabilization of lipid membranes of vesicles, resulting in vesicle rupture upon the application of a pulsed electric field. Electrofusion is a phenomenon wherein neighboring vesicles can be induced to fuse by exposing them to a pulsed electric field. We focus how the frequency of direct current (DC) pulses of electric field impacts rupture and electrofusion in cell-sized giant unilamellar vesicles (GUVs) prepared in a physiological buffer. The average time, probability, and kinetics of rupture and electrofusion in GUVs have been explored at frequency 500, 800, 1050, and 1250 Hz. The average time of rupture of many 'single GUVs' decreases with the increase in frequency, whereas electrofusion shows the opposite trend. At 500 Hz, the rupture probability stands at 0.45 ± 0.02, while the electrofusion probability is 0.71 ± 0.01. However, at 1250 Hz, the rupture probability increases to 0.69 ± 0.03, whereas the electrofusion probability decreases to 0.46 ± 0.03. Furthermore, when considering kinetics, at 500 Hz, the rate constant of rupture is (0.8 ± 0.1)×10-2 s-1, and the rate constant of fusion is (2.4 ± 0.1)×10-2 s-1. In contrast, at 1250 Hz, the rate constant of rupture is (2.3 ± 0.8)×10-2 s-1, and the rate constant of electrofusion is (1.0 ± 0.1)×10-2 s-1. These results are discussed by considering the electrical model of the lipid bilayer and the energy barrier of a prepore., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Bhuiyan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

To clarify the damage of lipid bilayer region in bacterial cell membrane caused by antimicrobial peptides (AMPs) and antimicrobial compounds (AMCs), their interactions with giant unilamellar vesicles (GUVs) of various lipid compositions have been examined. The findings revealed two main causes for the leakage: nanopore formation in the membrane and burst of GUVs. Although GUV burst has been explained previously based on the carpet model, the supporting evidence is limited. In this review, to better clarify the mechanism of GUV burst by AMPs, AMCs, and other membrane-active peptides, we described current knowledge of the conditions, characteristics, and detailed processes of GUV burst and the changes in the shape of the GUVs during burst. We identified several physical factors that affect GUV burst, such as membrane tension, electrostatic interaction, structural changes of GUV membrane such as membrane folding, and oil in the membrane. We also clarified one of the physical mechanisms underlying the instability of lipid bilayers that are associated with leakage in the carpet model. Based on these results, we propose a mechanism underlying some types of GUV burst induced by these substances: the growth of a nanopore to a micropore, resulting in GUV burst., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Integrating high-throughput phenotyping (HTP) based traits into phenomic and genomic selection (GS) can accelerate the breeding of high-yielding and climate-resilient wheat cultivars. In this study, we explored the applicability of Unmanned Aerial Vehicles (UAV)-assisted HTP combined with deep learning (DL) for the phenomic or multi-trait (MT) genomic prediction of grain yield (GY), test weight (TW), and grain protein content (GPC) in winter wheat. Significant correlations were observed between agronomic traits and HTP-based traits across different growth stages of winter wheat. Using a deep neural network (DNN) model, HTP-based phenomic predictions showed robust prediction accuracies for GY, TW, and GPC for a single location with R 2 of 0.71, 0.62, and 0.49, respectively. Further prediction accuracies increased (R 2 of 0.76, 0.64, and 0.75) for GY, TW, and GPC, respectively when advanced breeding lines from multi-locations were used in the DNN model. Prediction accuracies for GY varied across growth stages, with the highest accuracy at the Feekes 11 (Milky ripe) stage. Furthermore, forward prediction of GY in preliminary breeding lines using DNN trained on multi-location data from advanced breeding lines improved the prediction accuracy by 32% compared to single-location data. Next, we evaluated the potential of incorporating HTP-based traits in multi-trait genomic selection (MT-GS) models in the prediction of GY, TW, and GPC. MT-GS, models including UAV data-based anthocyanin reflectance index (ARI), green chlorophyll index (GCI), and ratio vegetation index 2 (RVI&#95;2) as covariates demonstrated higher predictive ability (0.40, 0.40, and 0.37, respectively) as compared to single-trait model (0.23) for GY. Overall, this study demonstrates the potential of integrating HTP traits into DL-based phenomic or MT-GS models for enhancing breeding efficiency., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Kaushal, Gill, Billah, Khan, Halder, Bernardo, Amand, Bai, Glover, Maimaitijiang and Sehgal.)

Developing countries with predominantly agricultural economies are particularly vulnerable to short-term climate variability and long-term climate change adversities. This provides the context for adopting climate-smart agricultural (CSA) practices to ensure consistent agricultural product supply and food security. Although concepts of CSA tools and strategies are well-established, their adoption trends in developing regions are still undesirable, with little to no empirical understanding of factors contributing to these trends. The current research aims to address this gap of knowledge by assessing the nature of climate change impacts, adaptation strategies, and socio-economic characteristics of the farmers influencing the adoption of CSA techniques in a rural cohort in Southwestern Bangladesh. Systematically designed interview schedules revealed that the most common and devastating consequences of climate change in the study area were increased infestation of pests and diseases. Other overlooked hazards including water-logging, cyclones and cold waves, are posing new threats to regional agriculture by changing conventional patterns. Contrary to the status quo, many CSA technologies, especially indigenous approaches, are widely and effectively adopted by farmers in the studied area to cope with climate change. These adoption tendencies and innovations in CSA were found to be significantly driven by factors such as extension and training programs, education of farmers, and dependence on on-farming income. The findings of the current study provide a preliminary analysis of the attitude, knowledge and trends of CSA technology adoption by farmers in a less-saline floodplain niche of a predominantly salinity-impacted coastal region. The study acts as a model for future relevant investigations and implementations for stakeholders with supply of inputs, technologies and expertise in finding sustainable solutions and appropriate extension for communities around the world confronted with similar challenges., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Fluoroquinolone (FQ) antibiotics have become a subject of growing concern due to their increasing presence in the environment, particularly in the soil and groundwater. This review provides a comprehensive examination of the attributes, prevalence, ecotoxicity, and remediation approaches associated with FQs in environmental matrices. The paper discusses the physicochemical properties that influence the fate and transport of FQs in soil and groundwater, exploring the factors contributing to their prevalence in these environments. Furthermore, the ecotoxicological implications of FQ contamination in soil and aquatic ecosystems are reviewed, shedding light on the potential risks to environmental and human health. The latter part of the review is dedicated to an extensive analysis of remediation approaches, encompassing both in-situ and ex-situ methods employed to mitigate FQ contamination. The critical evaluation of these remediation strategies provides insights into their efficacy, limitations, and environmental implications. In this investigation, a correlation between FQ antibiotics and climate change is established, underlining its significance in addressing the Sustainable Development Goals (SDGs). The study further identifies and delineates multiple research gaps, proposing them as key areas for future investigational directions. Overall, this review aims to consolidate current knowledge on FQs in soil and groundwater, offering a valuable resource for researchers, policymakers, and practitioners engaged in environmental management and public health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The photocatalytic activity of fluorine (F) doped CuO nanoparticles (NPs) prepared employing modified sol-gel process was investigated here in this study. Structural and elemental characterization using XRD and XPS data confirmed successful incorporation of F as dopant. F doping led to lattice distortion and reduced crystallinity with smaller crystallite size while promoting the emergence of Cu 2 O as the second phase. Morphological analysis showed irregularly shaped, fused particles with a decreasing particle size trend upon doping. Addition of hydrogen peroxide generated hydroxyl radicals (OH˙) under ultra-violet (UV) light, which effectively degrades pollutants by facilitating the photocatalytic kinetics. Photocatalytic activity of all the nanoparticles was examined against Rhodamine B (Rh B) dye and most efficient degradation (97.78%) was observed for 3 mol% F dopant concentration. The emergence of Cu 2 O phase for doping beyond 1 mol% F doped CuO might be the prime reason to enhance its degradation performance. Conversely, 5 mol% doping caused notable phase changes and decreased degradation rate (88.05%) due to increased recombination rate in presence of metallic copper. The ability of F doped CuO nanoparticles to disintegrate organic contaminants by producing reactive oxygen species when exposed to UV light suggests their potential effectiveness in applications such as dye degradation, water purification, and environmental sustainability., Competing Interests: There are no conflicts of interest to declare., (This journal is © The Royal Society of Chemistry.)

Most antimicrobial peptides (AMPs) induce pore formation and a burst of lipid bilayers and plasma membranes. This causes severe leakage of the internal contents and cell death. The AMP PGLa forms nanopores in giant unilamellar vesicles (GUVs) comprising dioleoylphosphatidylcholine (DOPC) and dioleoylphosphatidylglycerol (DOPG). We here elucidated the effect of the line tension of a prepore rim on PGLa-induced nanopore formation by investigating the interaction of PGLa with single GUVs comprising dioleoylphosphatidylethanolamine (DOPE)/DOPG (6:4) in buffer using the single GUV method. We found that PGLa forms nanopores in the GUV membrane, which evolved into a local burst and burst of GUVs. The rate of pore formation in DOPE/DOPG-GUVs was smaller than that in DOPC/DOPG-GUVs. PGLa is located only in the outer leaflet of a GUV bilayer just before a fluorescent probe AF647 leakage from the inside, indicating that this asymmetric distribution induces nanopore formation. PGLa-induced local burst and burst of GUVs were observed at 10 ms-time resolution. After nanopore formation started, dense particles and small vesicles appeared in the GUVs, followed by a decrease in the GUV diameter. The GUV was finally converted into smaller GUV or lipid membrane aggregates. We discuss the mechanisms of PGLa-induced nanopore formation and its direct evolution to a GUV burst.

The chemical co-precipitation method, an effective approach in the synthesis of nanomaterials, was used to synthesize CuO nanoparticles (NPs). Structural and morphological modification of undoped and nitrogen (N) doped CuO nanoparticles were studied thoroughly using X-ray diffraction (XRD), FT-IR and field emission scanning electron microscope (FE SEM). Doping effect on defects was investigated using X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and photoluminescence (PL) spectroscopy. Thus, the effect of doping on crystallinity, crystallite size, strain induced in lattice, defects and electron-hole recombination rate were investigated. Optical band gap was calculated using Kubelka-Munk function from the diffuse reflectance spectra (DRS) obtained using ultraviolet visible (UV-Vis) spectroscopy. Finally, photocatalytic performance was studied from rhodamine B (Rh B) degradation and reaction kinetics were analyzed. Maximum degradation efficiency was obtained for 1.0 mol% N doped CuO NPs which also exhibited minimum band gap and lowest electron-hole recombination rate. For the optimum doping concentration, nitrogen was found to create oxygen vacancies while substituting oxygen in the lattice, and thus reduce electron-hole recombination rate and increase photocatalytic degradation rate effectively., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors. Published by Elsevier Ltd.)

The comparative effect of serial stenosis and aneurysms arteries on blood flow is examined to identify atherosclerotic diseases. The finite element approach has been used to solve the continuity, momentum, and Oldroyd-B partial differential equations to analyze the blood flow. Newtonian and non-Newtonian both cases are taken for the viscoelastic response of blood. In this study, the impact of multiple stenotic and aneurysmal arteries on blood flow have been studied to determine the severity of atherosclerosis diseases through the analysis of blood behavior. The novel aspect of the study is its assessment of the severity of atherosclerotic disorders for the occurrence of serial stenosis and aneurysm simultaneously in the blood vessel wall in each of the four cases. The maximum abnormal arterial blood flow effect is found for the presence of serial stenoses compared to aneurysms which refers to the severity of atherosclerosis. At the hub of stenosis, the blood velocity magnitude and wall shear stress (WSS) are higher, whereas the arterial wall normal gradient values are lower. For all cases, the contrary results are observed at the hub of the aneurysmal model. The blood flow has been affected significantly by the increases in Reynolds number for both models. The influence of stenotic and aneurysmal arteries on blood flow is graphically illustrated in terms of the velocity profile, pressure distribution, and WSS. Medical experts may use this study's findings to assess the severity of cardiovascular diseases., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Microplastics (MPs), small synthetic particles, have emerged as perilous chemical pollutants in aquatic habitats, causing grave concerns about their disruptive effects on ecosystems. The fauna and flora inhabiting these specific environments consume these MPs, unwittingly introducing them into the intricate web of the food chain. In this comprehensive evaluation, the current methods of identifying MPs are amalgamated and their profound impacts on marine and freshwater ecosystems are discussed. There are many potential risks associated with MPs, including the dangers of ingestion and entanglement, as well as internal injuries and digestive obstructions, both marine and freshwater organisms. In this review, the merits and limitations of diverse identification techniques are discussed, including spanning chemical analysis, thermal identification, and spectroscopic imaging such as Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, and fluorescent microscopy. Additionally, it discusses the prevalence of MPs, the factors that affect their release into aquatic ecosystems, as well as their plausible impact on various aquatic ecosystems. Considering these disconcerting findings, it is imperative that appropriate measures should be taken to assess the potential risks of MP pollution, protect aquatic life and human health, and foster sustainable development., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The pore edge tension ( Γ ) of a membrane closely intertwines with membrane stability and plays a vital role in the mechanisms that facilitate membrane resealing following pore formation caused by electrical and mechanical tensions. We have explored a straightforward procedure to determine Γ by fitting the inverse of the tension-dependent logarithm of the rate constant of rupture of giant unilamellar vesicles (GUVs) using the Arrhenius equation. The GUVs were prepared using a combination of 1,2-dioleoyl- sn-glycero -3-phospho-(1'- rac -glycerol) (DOPG) and 1, 2-dioleoyl- sn-glycero -3-phosphocholine (DOPC) in a physiological environment. The effects of sugar concentration, membrane surface charge density, and membrane cholesterol concentration on Γ have been investigated. The values of Γ increase with sugar concentration in the physiological buffer, measuring 9.6 ± 0.3, 10.4 ± 0.1, and 16.2 ± 0.1 pN for 40, 100, and 300 mM, respectively. A higher concentration of anionic lipids (70 mol% of DOPG) significantly reduces Γ . An increasing trend of Γ with cholesterol content was observed; specifically, the values of Γ were 11.9 ± 0.9, 13.9 ± 0.7, and 16.2 ± 0.4 pN for 15, 29, and 40 mol% cholesterol, respectively. Thus, the presence of higher anionic lipids in the bilayer led to a decrease in membrane stability. In contrast, the presence of higher sugar concentrations in the buffer and increased cholesterol concentration in the membranes enhanced membrane stability.

The osmotic pressure (Π) method has recently been developed to quantitatively examine the effect of membrane tension (σ) on pore formation in giant unilamellar vesicles (GUVs) induced by antimicrobial peptides (AMPs). Here, we used the Π method to reveal the effect of σ on the interaction of an AMP, PGLa, with lipid bilayers comprising dioleoylphosphatidylglycerol (DOPG) and dioleoylphosphatidylcholine (DOPC) (4/6). PGLa induced leakage of fluorescent probes from single GUVs under Π, indicating nanopore formation. Membrane tension did not transform a PGLa-induced nanopore into a micropore nor cause GUV burst up to 3.4 mN/m, which is in contrast with the effect of σ on another AMP, magainin 2-induced pore formation, where lower σ resulted in GUV burst. The fraction of leaking GUVs at a specific time increased with increasing σ, indicating that the rate of PGLa-induced pore formation increases with increasing σ. The rate of transfer of fluorescent probe-labeled PGLa across the lipid bilayer without pore formation also increased with increasing σ. PGLa-induced pore formation requires a symmetric distribution of peptides in both leaflets of the GUV bilayer, and thus we infer that the increase in the rate of PGLa transfer from the outer leaflet to the inner leaflet underlies the increase in the rate of pore formation with increasing σ. On the basis of these results, we discuss the difference between the effect of σ on nanopore formation in GUV membranes induced by PGLa and that by magainin 2., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

The current investigation aims to choose an alternate potential replacement for the nonbiodegradable synthetic fibers used in polymer composites. This goal motivated the thorough characterization of Rosa hybrida bark (RHB) fibers. The research explored fiber characterization such as morphological, mechanical, thermal, and physical properties. The suggested fiber features a percentage of cellulose, hemicellulose molecules, and lignin of 52.99 wt%, 18.49 wt%, and 17.34 wt%, respectively according to chemical composition studies, which improves its mechanical properties. It is suitable for lightweight applications due to its decreased density (1.194 gcm -3 ). The purpose of the Fourier transform infrared spectroscope was to observe and record how various chemical groups were distributed throughout the surface of the fiber. The presence of 1.41 nm-sized crystalline cellulose and further XRD analysis showed a crystallinity index of 75.48 %. Scanning electron microscope studies revealed that RHB fibers have a rough surface. According to a single fiber tensile test, for gauge length (GL) 40 mm, Young's modulus and tensile strength of RHB fibers were 6.57 GPa and 352.01 MPa, respectively, and for GL 50 mm, 9.02 GPa and 311 MPa, respectively. Furthermore, thermo-gravimetric examination revealed that the isolated fibers were thermally stable up to 290 °C and the kinetic activation energy was found to be 75.32 kJ/mol. The fibers taken from the Rosa hybrida flower plants' bark exhibit qualities similar to those of currently used natural fibers, making them a highly promising replacement for synthetic fibers in polymer matrix composites., Competing Interests: Declaration of competing interest The authors affirm that they have no known financial or interpersonal conflicts that might have looked to have influenced the research presented in this study., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Positive membrane tension in the stretched plasma membrane of cells and in the stretched lipid bilayer of vesicles has been well analyzed quantitatively, whereas there is limited quantitative information on negative membrane tension in compressed plasma membranes and lipid bilayers. Here, we examined negative membrane tension quantitatively. First, we developed a theory to describe negative membrane tension by analyzing the free energy of lipid bilayers to obtain a theoretical equation for negative membrane tension. This allowed us to obtain an equation describing the negative membrane tension (σosm) for giant unilamellar vesicles (GUVs) in hypertonic solutions due to negative osmotic pressure (Π). Then, we experimentally estimated the negative membrane tension for GUVs in hypertonic solutions by measuring the rate constant (kr) of rupture of the GUVs induced by the constant tension (σex) due to an external force as a function of σex. We found that larger σex values were required to induce the rupture of GUVs under negative Π compared with GUVs in isotonic solution and quantitatively determined the negative membrane tension induced by Π (σosm) by the difference between these σex values. At small negative Π, the experimental values of negative σosm agree with their theoretical values within experimental error, but as negative Π increases, the deviation increases. Negative tension increased the stability of GUVs because higher tensions were required for GUV rupture, and the rate constant of antimicrobial peptide magainin 2-induced pore formation decreased., (© 2024 Author(s). Published under an exclusive license by AIP Publishing.)

Global warming is one of the greatest threats to living organisms. Among them, marine invertebrates are severely impacted on reproductive fitness by rising seawater surface temperatures due to climate change (e.g., massive heat waves). In this study, we used highly sensitive radioimmunoassay, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), in situ TUNEL assay, luminescence assay, and colorimetric assay techniques to investigate the impacts of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in gonads of Atlantic sea urchin (Arbacia punctulata, a commercially important species). Young adult sea urchins were exposed to 24, 28, and 32 °C for one week in a controlled laboratory setting. High temperatures (28 and 32 °C) markedly increased global DNA methylation (around 1.1-1.5-fold in testes and ~ 1.7-fold in ovaries) and 5-methylcytosine (5-mC) levels in gonads (around 2.7- to ~5.1-fold in ovaries and ~ 3.5- to ~6.2-fold in testes) compared with controls (24 °C). The number of apoptotic nuclei in gonads was much higher in high-temperature groups. The caspase activity also increased significantly (P < 0.05) in gonads in high-temperature groups. Nitrate/nitrites (NOx, a biomarker of reactive nitrogen species) levels were increased around 2.6- to ~5.2-fold in testes and ~ 1.9- to ~3.8-fold in ovaries in high-temperature groups. Collectively, these outcomes indicate that high temperatures drastically induce global DNA methylation, 5-mC expression levels, cellular apoptosis, and NOx levels in the gonads of Atlantic sea urchin., Competing Interests: Declaration of Competing Interest There are no conflicts of interest for this study., (Copyright © 2023 Elsevier Inc. All rights reserved.)