32 results on '"Bingqi Dong"'
Search Results
2. Identification of potential ferroptosis-associated biomarkers in rheumatoid arthritis
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Xu He, Juqi Zhang, Mingli Gong, Yanlun Gu, Bingqi Dong, Xiaocong Pang, Chenglong Zhang, and Yimin Cui
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rheumatoid arthritis ,ferroptosis ,GRN ,ENO1 ,PTGS2 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundRheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by inflammation and gradual joint degeneration, resulting in function disability. Recently, ferroptosis, a novel form of regulated cell death that involves iron-dependent lipid peroxidation, has been implicated in the pathogenesis of RA. However, the underlying molecular mechanisms and key genes involved in ferroptosis in RA remain largely unknown.MethodsThe GSE134420 and GSE77298 datasets were downloaded and DEGs were identified using R software. The DEGs were then mapped to the dataset of 619 ferroptosis-related genes obtained from the GeneCards database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to investigate the possible biological functions. Protein-protein interaction (PPI) networks were constructed to identify the hub genes. The relationship between hub genes and immune infiltration was estimated using the CIBERSORT algorithms. Gene Set Enrichment Analysis (GSEA) was used to explore the underlying signaling pathways of hub genes. Genome-wide association studies (GWAS) analysis was performed to confirm the pathogenic regions of the hub genes. RcisTarget and Gene-motif ranking databases were used to identify transcription factors (TFs) associated with the hub genes. The miRcode databases were utilized to construct the microRNA (miRNA)-messenger RNA (mRNA) network. Single-cell analysis was utilized to cluster cells and display the expression of hub genes in cell clusters. Finally, the expression and potential mechanism of hub genes were investigated in human and experimental samples.ResultsThree hub genes PTGS2, ENO1, and GRN highly associated with ferroptosis were identified. Four pathogenic genes HLA-B, MIF, PSTPIP, TLR1 were identified that were significantly and positively correlated with the expression levels of hub genes. The results of the GSEA showed that the hub genes were significantly enriched in pathways related to immunity, lysosome, phagocytosis and infection. ENO1 and PTGS2 were enriched in the TF-binding motif of cisbp_M5493. The hub genes were validated in experimental and patient samples and highly level of ENO1 expression was found to inhibit ACO1, which reduces ferroptosis in proliferating fibroblast-like synoviocytes (FLS).ConclusionPTGS2, ENO1 and GRN were identified and validated as potential ferroptosis-related biomarkers. Our work first revealed that ENO1 is highly expressed in RA synovium and that ferroptosis may be regulated by the ENO1-ACO1 axis, advancing the understanding of the underlying ferroptosis-related mechanisms of synovial proliferation and providing potential diagnostic and therapeutic targets for RA.
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- 2023
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3. Risk of venous thromboembolism with janus kinase inhibitors in inflammatory immune diseases: a systematic review and meta-analysis
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Juqi Zhang, Wenhui Li, Mingli Gong, Yanlun Gu, Hanxu Zhang, Bingqi Dong, Qi Guo, Xiaocong Pang, Qian Xiang, Xu He, and Yimin Cui
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janus kinase inhibitor ,venous thromboembolism ,inflammatory immune diseases ,meta-analysis topic: venous thromboembolism ,deep vein thrombosis ,pulmonary embolism ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Objectives: This study aimed to evaluate the risk of venous thrombosis (VTE) associated with Janus kinase (JAK) inhibitors in patients diagnosed with immune-mediated inflammatory diseases.Methods: We conducted a comprehensive search of PUBMED, Cochrane, and Embase databases for randomized controlled trials evaluating venous thromboembolic incidence after administering JAK inhibitors in patients with immune-mediated inflammatory diseases. The studies were screened according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, and a meta-analysis was performed.Results: A total of 16 studies, enrolling 17,242 participants, were included in this review. Four approved doses of JAK inhibitors were administered in the included studies. The meta-analysis revealed no significant difference in the incidence of VTE between patients receiving JAK inhibitors, a placebo, or tumor necrosis factor (TNF) inhibitors (RR 0.72, 95% CI (0.33-1.55); RR 0.94, 95%CI (0.33-2.69)). Subgroup analysis showed a lower risk of VTE with lower doses of JAK inhibitors [RR 0.56, 95%CI (0.36-0.88)]. Compared with the higher dose of tofacitinib, the lower dose was associated with a lower risk of pulmonary embolism [RR 0.37, 95%CI (0.18-0.78)].Conclusion: Our meta-analysis of randomized controlled trials observed a potential increase in the risk of VTE in patients with immune-mediated inflammatory diseases treated with JAK inhibitors compared to placebo or tumor necrosis factor inhibitors, though statistical significance was not attained. Notably, a higher risk of pulmonary embolism was observed with high doses of tofacitinib. Our findings provide valuable insights for physicians when evaluating the use of JAK inhibitors for patients with immune-mediated inflammatory diseases.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023382544, identifier CRD42023382544
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- 2023
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4. A novel immunogenic cell death–related subtype classification and risk signature for predicting prognosis and immunotherapy efficacy in gastric cancer
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Bingqi Dong, Yingchao Wu, Junling Zhang, Yanlun Gu, Ran Xie, Xu He, Xiaocong Pang, Xin Wang, and Yimin Cui
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immunogenic cell death ,prognosis ,tumor microenvironment ,immunotherapy ,gastric cancer ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The majority of gastric cancer (GC) patients are in a progressive stage at the initial stage of treatment, and the overall response rate to immunotherapy remains unsatisfactory largely due to the lack of effective prognostic biomarkers. Immunogenic cell death (ICD) was identified as a new form of regulated cell death that can activate adaptive immune responses and further promote immunotherapy efficacy. Therefore, we attempted to characterize the ICD-associated signature to stratify patients who could benefit from immunotherapy. In our study, two subgroups of patients were identified based on the data of 34 ICD-related genes extracted from The Cancer Genome Atlas database via consensus clustering. The estimated scores, stromal scores, immune scores, tumor purity, and survival rate showed significant differences between the low and high ICD groups. Then, we constructed an ICD-related risk signature, including IFNB1, IL6, LY96, and NT5E, using least absolute shrinkage and selection operator Cox regression analysis; then, high- and low-risk groups could be clearly distinguished. Notably, the risk score is a reliable predictor of the prognosis and immunotherapy outcome in GC, which was further validated in an immunohistochemistry assay. These results suggest that ICD is closely associated with the prognosis and tumor immune microenvironment in GC. Taken together, this study first constructed and validated a prognostic ICD-related signature to predict the survival and effect of immunotherapy in GC, which provided new insight for potent individualized immunotherapy strategies.
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- 2023
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5. The challenges and opportunities of αvβ3-based therapeutics in cancer: From bench to clinical trials
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Yanlun Gu, Bingqi Dong, Xu He, Zhiwei Qiu, Juqi Zhang, Mo Zhang, Haitao Liu, Xiaocong Pang, and Yimin Cui
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Integrin ,αvβ3 ,RGD ,Cancer ,Extracellular matrix ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Integrins are main cell adhesion receptors serving as linker attaching cells to extracellular matrix (ECM) and bidirectional hubs transmitting biochemical and mechanical signals between cells and their environment. Integrin αvβ3 is a critical family member of integrins and interacts with ECM proteins containing RGD tripeptide sequence. Accumulating evidence indicated that the abnormal expression of integrin αvβ3 was associated with various tumor progressions, including tumor initiation, sustained tumor growth, distant metastasis, drug resistance development, maintenance of stemness in cancer cells. Therefore, αvβ3 has been explored as a therapeutic target in various types of cancers, but there is no αvβ3 antagonist approved for human therapy. Targeting-integrin αvβ3 therapeutics has been a challenge, but lessons from the past are valuable to the development of innovative targeting approaches. This review systematically summarized the structure, signal transduction, regulatory role in cancer, and drug development history of integrin αvβ3, and also provided new insights into αvβ3-based therapeutics in cancer from bench to clinical trials, which would contribute to developing effective targeting αvβ3 agents for cancer treatment.
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- 2023
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6. A laparoscopic radical inguinal lymphadenectomy approach partly preserving great saphenous vein branches can benefit for patients with penile carcinoma
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Yongkang Ma, Jianwei Hao, Huaqi Yin, Mingkai Zhu, Bao Guan, Chaoshuai Zhu, Bingqi Dong, Shiming Zhao, Zhaohong He, and Tiejun Yang
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Penile carcinoma ,Inguinal lymphadenectomy ,Laparoscope ,Great saphenous vein branches ,Surgery ,RD1-811 - Abstract
Abstract Background Inguinal lymphadenectomy (iLAD) is effective for penile carcinoma treatment, but usually results in many complications. This study aims to clinically evaluate the feasibility and clinical significance of a laparoscopic radical iLAD approach partly preserving great saphenous vein branches for penile carcinoma patients. Methods A total of 48 patients with penile cancer who underwent laparoscopic radical iLAD with retention of the great saphenous vein in Henan Cancer Hospital from 2012 Jan to 2020 Dec were included in this study. Sixteen penile carcinoma patients who underwent laparoscopic radical iLAD preserving parts of superficial branches of the great saphenous vein were identified as the sparing group, and the matched 32 patients who incised those branches were identified as control group. This new procedure was performed by laparoscopy, preserving parts of superficial branches of the great saphenous vein, superficial lateral and medial femoral veins. Clinicopathological features and perioperative variables were recorded. Postoperative complications, including skin flap necrosis, lymphorrhagia, and lower extremity edema were analyzed retrospectively. Results We found that the operative time of the sparing group is significantly longer than the control group (p = 0.011). There was no statistical difference in intraoperative blood loss, the lymph node number per side, average time to remove the drainage tube and postoperative hospital stay between the two groups. Compared to the control group, the sparing group showed a significantly decreased incidence of lower extremity edema (p = 0.018). The preservation of parts of superficial branches of the great saphenous vein was mainly decreased the incidence of edema below ankle (p = 0.034). Conclusions This study demonstrated that the iLAD with preserving parts of superficial branches of the great saphenous vein, with a decreased incidence of postoperative complications, is a safe and feasible approach for penile cancer.
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- 2022
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7. Case report: Gemcitabine intravesical hyperthermic infusion combined with tislelizumab in muscle invasive bladder urothelium carcinoma
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Zheng Du, Huaqi Yin, Shiming Zhao, Yongkang Ma, Zhenghui Sun, Bingqi Dong, Mingkai Zhu, Chaoshuai Zhu, Jiangshan Peng, and Tiejun Yang
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muscle invasive bladder urothelium carcinoma ,chemotherapy ,bladder hyperthermia ,gemcitabine ,tislelizumab ,tumor immunotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundMuscle invasive bladder urothelium carcinoma is a common urinary tract tumor. With the deepening of research, more and more treatment methods are applied in clinical practice, extending the life of patients. Among them, the clinical application of chemotherapeutic intravesical hyperthermia and tumor immunotherapy provides new ideas for our treatment.Case reportAn 81-year-old female patient was diagnosed with stage T2N0M0 bladder cancer in our hospital. Because the patient and her family were keen to preserve her bladder, they declined surgery and opted for combined chemotherapy. After informed consent from the patient and her family, she received cisplatin combined with gemcitabine intravesical hyperthermic infusion. But the side effects of cisplatin made her intolerable to chemotherapy. With their informed consent we changed her to intravenous tislelizumab in combination with gemcitabine intravesical hyperthermic infusion to continue her treatment. During the subsequent follow-up visits, we found a surprising effect of the treatment.ConclusionGemcitabine intravesical hyperthermia therapy combined with intravenous tislelizumab in the treatment of muscle invasive bladder urothelium carcinoma may provide a new possible therapeutic strategy of some patients who are inoperable or refuse surgery.
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- 2022
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8. Penile cancer with bilateral inguinal lymph node metastases surviving for more than 4 years: A case report
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Mingkai Zhu, Huaqi Yin, Bingqi Dong, and Tiejun Yang
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Penile cancer ,Immunotherapy ,Chemotherapy ,Targeted therapy ,Surgery ,RD1-811 - Published
- 2023
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9. Clinical significance of circulating tumour cells and Ki-67 in renal cell carcinoma
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Jinbo Song, Zhe Yu, Bingqi Dong, Mingkai Zhu, Xiaofeng Guo, Yongkang Ma, Shiming Zhao, and Tiejun Yang
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Circulating tumour cells (CTCs) ,Circulating tumour microemboli (CTM) ,Perioperative period ,Renal cell carcinoma ,Ki-67 ,Surgery ,RD1-811 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Renal cell carcinoma (RCC) is a common malignant tumour of the genitourinary system. We aimed to analyse the potential value of metastasis-related biomarkers, circulating tumour cells (CTCs) and the proliferative marker Ki-67 in the diagnosis of RCC. Methods Data from 24 laparoscopic radical nephrectomies (RNs) and 17 laparoscopic partial nephrectomies (PNs) were collected in 2018. The numbers and positive rates of CTCs and circulating tumour microemboli (CTM) in the peripheral blood were obtained at three different time points: just before surgery, immediately after surgery and 1 week after surgery. Ki-67 protein expression was evaluated in the RCC tissue by immunohistochemistry. Results Except for the statistically significant association between the preoperative CTC counts and tumour size, no association between the number and positive rate of perioperative CTCs and clinicopathological features was found. The CTC counts gradually decreased during the perioperative period, and at 1 week after surgery, they were significantly lower than those before surgery. High Ki-67 expression was significantly positively correlated with preoperative CTC counts. In addition, Ki-67 expression was higher in the high CTC group (≥ 5 CTCs). Conclusion Our results suggest that surgical nephrectomy is associated with a decrease in CTC counts in RCC patients. CTCs can act as a potential biomarker for the diagnosis and prognosis of RCC. A careful and sufficient long-term follow-up is needed for patients with high preoperative CTC counts.
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- 2021
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10. The Atmospheric Pollution Characteristics and Health Risk Assessment of Perfluorohexane Sulfonic Acid in Beijing
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Fan Wang, Jing Wu, Yiru Zhuang, Bingqi Dong, Yueling Zhang, and Lin Peng
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perfluorohexane sulfonate acid ,perfluoroalkyl and polyfluoroalkyl substances ,gas–particle partitioning ,deposition ,health risk assessment ,Meteorology. Climatology ,QC851-999 - Abstract
Perfluorohexane sulfonic acid (PFHxS), one of the substitutes for perfluorooctane sulfonic acid (PFOS), has been widely discussed around the world. In this study, the atmospheric concentrations of PFHxS in Beijing in the summer and autumn of 2021 were monitored, and the gas–particle partitioning and wet and dry deposition characteristics were analyzed. The results show that the average total concentrations of PFHxS in Beijing in the summer and autumn were 1.61 pg/m3 and 1.41 pg/m3, respectively. The particulate fraction φ of PFHxS was about 48% during the research period, and there was a significant positive correlation between φ and air temperatures. The linear fitting relationship between logKp(gas–particle partition coefficient) and logPL (compound vapor pressure) showed that the absorption between the PFHxS molecule and particulate matter dominated the process of gas–particle partitioning. The total deposition flux of PFHxS was the largest in July and the smallest in October, with values of 55.2 and 23.7 ng/m2, respectively. During the study period, PFHxS in the atmosphere had no obvious health impacts on humans. However, with their extensive use, the possible harmful effects on children should be continuously investigated.
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- 2023
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11. In ovo feeding of epidermal growth factor: embryonic expression of intestinal epidermal growth factor receptor and posthatch growth performance and intestinal development in broiler chickens1
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Emily Kim, Nadeem Akhtar, Julang Li, Qianru Hui, Bingqi Dong, Chengbo Yang, and Elijah G. Kiarie
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broiler ,in ovo feeding ,epidermal growth factor ,intestinal development and function ,Animal culture ,SF1-1100 - Abstract
We investigated efficacy of in ovo application of epidermal growth factor (EGF) on intestinal expression of EGF receptor (EGFR) during embryogenesis (experiment 1) and posthatch growth performance and gastrointestinal development in broiler chickens (experiment 2). In experiment 1, 450 fertile Ross 708 eggs were allocated to 3 groups (150 eggs/group): 1) control, 2) 160 μg EGF/kg of egg, and 3) 640 μg of EGF/kg of egg. Eggs were candled for live embryos on day 16 and injected with the respective treatment solutions on day 17 and sampled for jejunal tissue from day 17 to hatch for EGFR analyses. There was no effect of EGF (P > 0.05) on EGFR expression on day 17 to 20; however, on day 21, EGF increased (P < 0.05) EGFR expression in EGF birds relative to control birds. In experiment 2, 600 fertile Ross 708 eggs were allocated to 5 treatments: 1) intact, no puncture or injection, 2) punched but not injected, 3) control, no EGF, 4) 80 μg of EGF/kg of egg, and 5) 160 μg of EGF/kg of egg. The eggs were incubated and candled for live embryos on D 19, treated, and subsequently transferred to the hatcher. Upon hatching, chicks were weighed, and 90 chicks per treatment placed in cages (15 birds/cage) and allowed free access to a standard antibiotic-free corn-soybean diet for 21 D. Feed intake and body weight were monitored on a weekly basis. Samples of birds were necropsied on D 0, 7, 14, and 21 for measurements of intestinal weight and jejunal histomorphology and excreta samples taken on D 3 to 5 and 17 to 19 for apparent retention of dry matter. There was no EGF effect (P > 0.05) on any posthatch response criteria. In conclusion, in ovo application of EGF increased EGFR expression but had no effect on posthatch growth performance, DM retention, and intestinal development. The lack of EGF effect on posthatch response was surprising but suggested in ovo application of EGF may not be a viable approach.
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- 2020
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12. Capsaicin Attenuates Lipopolysaccharide-Induced Inflammation and Barrier Dysfunction in Intestinal Porcine Epithelial Cell Line-J2
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Xiaoya Zhao, Bingqi Dong, Marissa Friesen, Shangxi Liu, Changqing Zhu, and Chengbo Yang
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capsaicin ,LPS induction ,IPEC-J2 ,inflammation ,TLR4/NF-κB pathway ,gut integrity ,Physiology ,QP1-981 - Abstract
Capsaicin is a spicy, highly pungent, colorless, vanilloid compound found in chili peppers with anti-inflammatory, antioxidant, anti-cancer, and analgesic properties. However, the protective effects of capsaicin on the pig intestine during inflammation are yet to be explored. This study investigated the effects of capsaicin on the gut inflammatory response, intestinal epithelial integrity, and gene expression level of nutrient transporters in a model of lipopolysaccharide (LPS)-induced inflammation in non-differentiated intestinal porcine epithelial cell line-J2 (IPEC-J2). The results showed that the pre-treatment of cells with capsaicin (100 μM) significantly decreased the gene expression and secretion of proinflammatory cytokines induced by LPS through Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. In addition, pre-treatment of cells with capsaicin also increased both gene and protein abundance of tight junction proteins. Furthermore, pre-treatment cells with capsaicin significantly increased trans-epithelial electrical resistance (TEER) and decreased permeability of fluorescein isothiocyanate-dextran (FD4) from the apical side to the basolateral side compared with the control (P < 0.05). Additionally, pre-treatment of cells with capsaicin upregulated the mRNA abundance of nutrients transporters such as Na+/glucose cotransporter 1 (SGLT1). These results suggested that capsaicin could attenuate LPS-induced inflammation response through TLR4/NF-κB pathway and improve barrier integrity and glucose absorption.
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- 2021
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13. Tumor Expression Profile Analysis Developed and Validated a Prognostic Model Based on Immune-Related Genes in Bladder Cancer
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Bingqi Dong, Jiaming Liang, Ding Li, Wenping Song, Shiming Zhao, Yongkang Ma, Jinbo Song, Mingkai Zhu, and Tiejun Yang
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bladder cancer ,immune-related signature ,The Cancer Genome Atlas ,Gene Expression Omnibus ,immunotherapy ,Genetics ,QH426-470 - Abstract
Background: Bladder cancer (BLCA) ranks 10th in incidence among malignant tumors and 6th in incidence among malignant tumors in males. With the application of immune therapy, the overall survival (OS) rate of BLCA patients has greatly improved, but the 5-year survival rate of BLCA patients is still low. Furthermore, not every BLCA patient benefits from immunotherapy, and there are a limited number of biomarkers for predicting the immunotherapy response. Therefore, novel biomarkers for predicting the immunotherapy response and prognosis of BLCA are urgently needed.Methods: The RNA sequencing (RNA-seq) data, clinical data and gene annotation files for The Cancer Genome Atlas (TCGA) BLCA cohort were extracted from the University of California, Santa Cruz (UCSC) Xena Browser. The BLCA datasets GSE31684 and GSE32894 from the Gene Expression Omnibus (GEO) database were extracted for external validation. Immune-related genes were extracted from InnateDB. Significant differentially expressed genes (DEGs) were identified using the R package “limma,” and Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis for the DEGs were performed using R package “clusterProfiler.” Least absolute shrinkage and selection operator (LASSO) regression analysis were used to construct the signature model. The infiltration level of each immune cell type was estimated using the single-sample gene set enrichment analysis (ssGSEA) algorithm. The performance of the model was evaluated with receiver operating characteristic (ROC) curves and calibration curves.Results: In total, 1,040 immune-related DEGs were identified, and eight signature genes were selected to construct a model using LASSO regression analysis. The risk score of BLCA patients based on the signature model was negatively correlated with OS and the immunotherapy response. The ROC curve for OS revealed that the model had good accuracy. The calibration curve showed good agreement between the predictions and actual observations.Conclusions: Herein, we constructed an immune-related eight-gene signature that could be a potential biomarker to predict the immunotherapy response and prognosis of BLCA patients.
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- 2021
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14. Identification of a Prognostic Signature Associated With the Homeobox Gene Family for Bladder Cancer
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Bingqi Dong, Jiaming Liang, Ding Li, Wenping Song, Jinbo Song, Mingkai Zhu, Shiming Zhao, Yongkang Ma, and Tiejun Yang
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bladder cancer ,homeobox gene family ,prognostic signature ,immunotherapy ,biomarkers ,Biology (General) ,QH301-705.5 - Abstract
Background: Bladder cancer (BLCA) is a common malignant tumor of the genitourinary system, and there is a lack of specific, reliable, and non-invasive tumor biomarker tests for diagnosis and prognosis evaluation. Homeobox genes play a vital role in BLCA tumorigenesis and development, but few studies have focused on the prognostic value of homeobox genes in BLCA. In this study, we aim to develop a prognostic signature associated with the homeobox gene family for BLCA.Methods: The RNA sequencing data, clinical data, and probe annotation files of BLCA patients were downloaded from the Gene Expression Omnibus database and the University of California, Santa Cruz (UCSC), Xena Browser. First, differentially expressed homeobox gene screening between tumor and normal samples was performed using the “limma” and robust rank aggregation (RRA) methods. The mutation data were obtained with the “TCGAmutation” package and visualized with the “maftools” package. Kaplan–Meier curves were plotted with the “survminer” package. Then, a signature was constructed by logistic regression analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using “clusterProfiler.” Furthermore, the infiltration level of each immune cell type was estimated using the single-sample gene set enrichment analysis (ssGSEA) algorithm. Finally, the performance of the signature was evaluated by receiver-operating characteristic (ROC) curve and calibration curve analyses.Results: Six genes were selected to construct this prognostic model: TSHZ3, ZFHX4, ZEB2, MEIS1, ISL1, and HOXC4. We divided the BLCA cohort into high- and low-risk groups based on the median risk score calculated with the novel signature. The overall survival (OS) rate of the high-risk group was significantly lower than that of the low-risk group. The infiltration levels of almost all immune cells were significantly higher in the high-risk group than in the low-risk group. The average risk score for the group that responded to immunotherapy was significantly lower than that of the group that did not.Conclusion: We constructed a risk prediction signature with six homeobox genes, which showed good accuracy and consistency in predicting the patient’s prognosis and response to immunotherapy. Therefore, this signature can be a potential biomarker and treatment target for BLCA patients.
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- 2021
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15. Review on Per- and Poly-Fluoroalkyl Substances’ (PFASs’) Pollution Characteristics and Possible Sources in Surface Water and Precipitation of China
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Fan Wang, Yiru Zhuang, Bingqi Dong, and Jing Wu
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i-PFASs ,China ,river ,lake ,precipitation ,the Tibetan Plateau ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
In recent years, due to the production and use of per- and poly-fluoroalkyl substances (PFASs), the research on the pollution characteristics and sources of PFASs in surface water and precipitation in China has attracted increasing attention. In this study, the related published articles with sampling years from 2010 to 2020 were reviewed, and the concentration levels, composition characteristics and possible sources of PFASs in surface water (rivers and lakes) and precipitation in China were summarized, including those in the Tibetan Plateau region. The results show that the concentrations of PFASs in surface water in different areas of China vary greatly, ranging from 0.775 to 1.06 × 106 ng/L. The production processes of fluorinated manufacturing facilities (FMFs) and sewage discharge from wastewater treatment plants (WWTPS) were the main sources of PFASs in surface water in China, and the concentrations of PFASs in water flowing through cities with high urbanization increased significantly compared with those before water flowed through cities with high urbanization. The compositions of PFASs in surface water gradually changed from long-chain PFASs, such as per-fluoro-octanoic acid (PFOA) and per-fluoro-octanesulfonic acid (PFOS) to short-chain PFASs, such as per-fluorobutanoic acid (PFBA), per-fluorobutanesulfonic acid (PFBS), perfluorohexanoic acid (PFHxA) and per-fluoropentanoic acid (PFPeA). The concentrations of PFASs in precipitation in China ranged from 4.2 to 191 ng/L, which were lower than those of surface water. The precipitation concentrations were relatively high around a fluorination factory and in areas with high urbanization levels. PFASs were detected in the surface water and precipitation in the Tibetan Plateau (TP), which is the global “roof of the world”, but the concentrations were low (0.115–6.34 ng/L and 0.115–1.24 ng/L, respectively). Local human activities and surface runoff were the main sources of PFASs in the surface water of the Tibetan Plateau. In addition, under the influence of the Southeast Asian monsoon in summers, marine aerosols from the Indian Ocean and air pollutants from human activities in Southeast Asia and South Asia will also enter the water bodies through dry and wet depositions. With the melting of glaciers caused by global warming, the concentration of PFASs in the surface water of the TP was higher than that before the melting of glaciers flowed into the surface water of the TP. Generally, this study summarized the existing research progress of PFAS studies on surface water and precipitation in China and identified the research gaps, which deepened the researchers’ understanding of this field and provided scientific support for related research in the future. The concentrations of PFASs in the water bodies after flowing through FMFs were significantly higher than those before water flowed through FMFs, so the discharge of the FMF production process was one of the main sources of PFASs in surface water.
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- 2022
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16. Birth Weight and Nutrient Restriction Affect Jejunal Enzyme Activity and Gene Markers for Nutrient Transport and Intestinal Function in Piglets
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Michael O. Wellington, Lucas A. Rodrigues, Qiao Li, Bingqi Dong, Josiane C. Panisson, Chengbo Yang, and Daniel A. Columbus
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low birth weight ,normal birth weight ,neonatal piglets ,intestinal development ,gene expression ,normal nutrition ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Significant variation in the birth weight of piglets has arisen due to increased sow prolificacy. Intestinal development and function may be affected by birth weight. Low birth weight (LBW) pigs may also have reduced feed intake, leading to further impairment of intestinal development. The objective of this study was to examine the intestinal development pattern of LBW and normal birth weight (NBW) piglets with normal nutrition (NN) or restricted nutrition (RN) in the pre-weaning period. Jejunal intestinal samples were analyzed for target gene expression and enzyme activity at d 28 (weaning) and d 56 (post-weaning). At d 28, excitatory amino acid transporter (EAAC1) and sodium-dependent neutral amino acid transporter (B0AT1) were downregulated in LBW compared to NBW pigs (p < 0.05). On d 56, B0AT1 and ASCT2 (glutamine transporter) were downregulated in RN compared to NN pigs (p < 0.05), regardless of birth weight. Peptide transporter 1 (PepT1) expression was downregulated in LBW compared to NBW pigs at 28 d (p < 0.05), with no effects of treatments at 56 d. Sodium-glucose transporter-1 (SGLT1) was upregulated in NBW-NN compared to LBW-NN pigs (p < 0.05) at 28 d. Alkaline phosphatase (ALP) was upregulated in LBW-RN at d 28. At d 56, claudin-3 (CLDN-3) and Zonular occludin-1 (ZO-1) were upregulated in NN compared to RN pigs (p < 0.05). There were no treatment effects on ALP, maltase, or sucrase activity at 28 d. However, at 56 d, ALP was upregulated in NBW-NN pigs while sucrase activity was upregulated in NN pigs (p < 0.05). The results demonstrate differences in jejunal gene expression associated with birth weight, with reduced gene expression of amino acid transporters (PepT1, EAAC1, B0AT1) in LBW compared to NBW pigs (p < 0.05). While neonatal nutrient restriction had minimal effects at 28 d and d 56 for tight junction protein transcript abundance, neutral amino acid transporter abundance was upregulated in NN pigs compared to RN piglets (p < 0.05).
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- 2021
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17. Hyperthermia inhibits cellular function and induces immunogenic cell death in renal cell carcinoma
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Huaqi, Yin, Bingqi, Dong, Yanhui, Zhao, Yongkang, Ma, Shiming, Zhao, Zhenghui, Sun, Zheng, Du, Jiangshan, Peng, and Tiejun, Yang
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- 2023
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18. Correction: The significance of transrectal ultrasound and urologist_dually guided pelvic floor muscle exercise in improving urinary continence after radical prostatectomy
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Huaqi, Yin, Zheng, Du, Yongkang, Ma, Shiming, Zhao, Zhenghui, Sun, Zhiwei, Wang, Congyu, Li, Qian, Li, Bingqi, Dong, Mingkai, Zhu, Chaoshuai, Zhu, Jiangshan, Peng, and Tiejun, Yang
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- 2023
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19. The significance of transrectal ultrasound and urologist_dually guided pelvic floor muscle exercise in improving urinary continence after radical prostatectomy
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Huaqi, Yin, Zheng, Du, Yongkang, Ma, Shiming, Zhao, Zhenghui, Sun, Zhiwei, Wang, Congyu, Li, Qian, Li, Bingqi, Dong, Mingkai, Zhu, Chaoshuai, Zhu, Jiangshan, Peng, and Tiejun, Yang
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- 2023
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20. Accelerated release of PFASs from glacier melting on the Qinghai-Tibet Plateau caused by global warming
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Lin Peng, Jing Wu, Bingqi Dong, Yiru Zhuang, Fan Wang, Lixin Yang, Yulong Yan, Junjie Li, Kai Xie, Dayu Zhang, Zhuocheng Liu, and Xiaolin Duan
- Abstract
Due to the characteristics of persistence, bioaccumulation, potential for long-range environmental transport and adverse effects, the emissions and pollution characteristics of long-chain, short-chain and ultra-short-chain polyfluoroalkyl and polyfluoroalkyl substances (PFASs) are affected by International attention, especially in the Arctic, Antarctic and the third pole Qinghai-Tibet Plateau region. The Tibetan Plateau is the highest plateau in the world (averaging over 4,000 metres) and has the largest ice reserves (approximately 7,481 cubic kilometres) except for the polar regions, and is known as the water tower of Asia. Kwok et al. (2013) found that glaciers can act as temporary reservoirs for PFASs, which are released by melting glaciers under the influence of global warming. Chen et al. (2019) found that melting glaciers have become the second major source of PFASs in Lake Nam Co. Since the 1980s, global warming has led to the retreat of more than 80% of the glaciers and the expansion of more than 50% of the lakes on the Tibetan Plateau, which may intensify the release of PFASs from the glaciers. In this study, 17 water samples, 12 sediment samples and 12 soil samples were collected in the east, south, west or north direction, or in the center of Lake Nam Co in August 2020. Moreover, 23 water samples were collected from glaciers and non-glacial runoff around the lake that flow into Lake Namco. 19 PFASs (C2-C18), and their nine isomers and one main precursor FHxSA were analyzed by solid-phase extraction (two-fraction elution) and ultra-high performance liquid chromatography/tandem mass spectrometry. The results showed the concentrations of PFASs in water bodies such as lake water, glacial runoff and non-glacial runoff were the highest, with average concentrations of 3603 pg/L, 9,823 pg/L and 4,089 pg/L, respectively, which were 4 times, 7.4 and 5.6 times those of 2017. The concentrations of PFASs in soil and sediment were significantly lower than those in water bodies, 1.86 and 0.616 ng/g (dry weight), respectively. It was estimated that the PFASs input flux of surface runoff to lake reached 18,926.5mg/d, of which the glacial runoff reached 11,326.2mg/d (7.7 times that of 2017). It is very likely that the melting of glaciers accelerated the release of PFASs from glacier runoff into the water of Lake Nam Co. For all the three media, the linear chain was the most important isomer of PFOA and PFOS, and the order of the proportion of branched chain isomers was consistent (iso->5m->4m->3m-PFOS/PFOA). The PFBA concentrations were highest in lakes and surface runoff, accounting for 55.2% and 81.2% of total PFASs, respectively. PFBA and other PFASs in lake water were poorly correlated, and Cai et al. (2012) found similar results in polar glaciers, suggesting that the PFBA in the Lake Nam Co probably mainly came from the melting of glaciers. In the future, global warming may further accelerate the melting of glaciers and the release of PFASs in glaciers, and the changing trend of PFASs concentrations in water bodies on the Tibetan Plateau should be continuously tracked.
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- 2023
21. Atmospheric observation and source analysis of CFCs, HCFCs and HFCs at the Medog background site on the southern slope of the Himalayas, China
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Jing Wu, Zehua Liu, Bo Yao, Minde An, Yueyuan Niu, Wanglin Zhao, Haibo Yu, Tong Wang, Bingqi Dong, and Lin Peng
- Abstract
The ozone-depleting substances chlorofluorocarbons (CFCs), hydrochlorofluorocarbons (HCFCs) and their major substitutes hydrofluorocarbons (HFCs) are all potent greenhouse gases, and their atmospheric concentrations and emission sources have received international attention. The relevant studies have been carried out at multiple background sites around the world, but there is no report on the southern slope of the Himalayas. For the first time, this study set up the Medog background observation site 30 km away from Medog County on the southern slope of the Himalayas (95.5262ºE, 29.5314ºN, 1298.8 m above sea level) in China. From August 10 to September 19 and November 3 to November 30 in 2021, 114 atmospheric samples were collected instantaneously using evacuated electro-polished stainless-steel canisters and manual pressurization equipment and 1-3 samples were collected per day. Nine substances (CFC-11, 12, 113; HCFC-22, 141b, 142b; HFC-23, 125, 134a) were analyzed using a high-precision measurement system of halogenated gases (ODS5-pro). For each substance, all the atmospheric samples were divided into two categories: background samples and polluted samples. The background samples are those whose concentrations were within the range of the background concentrations (measured at the Ragged Point site at the same latitude in 2021) ± 3σ (instrument precision), and the others were classified as polluted samples. Our results showed that the number of the polluted samples of each substance accounted for about 40%-90%. In addition, we found that the mixing ratios of CFC-113, HFC-23, and HCFC-22 in the polluted samples were 93, 44 and 311 pptv, respectively, with higher mixing ratios (31.6%, 24.5%, and 22.5%) above the background levels than other substances (4.3%~10.2%). Furthermore, CFC-113 and HFC-23 were significantly correlated (R = 0.429, P < 0.01), suggesting that they may have similar sources. Both Backward Trajectory and Potential Source Contribution Function of the polluted samples of CFC-113, HFC-23, and HCFC-22 found that the polluted air masses mainly came from the northeast of India and other regions southwest of the sampling site. In contrast, the background air masses mainly came from the local areas. The concentrations of CFC-113, HFC-23, and HCFC-22 in the polluted samples observed in this study, likely from the northeast of India, were about 20-30% higher than the results of aircraft samples conducted by Say et al. (2019) over Northern and Southern India in 2016. In the future, we will continue to carry out atmospheric observations at the Medog background site and try to use the suitable atmospheric transport model to inverse the emissions in the surrounding areas.
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- 2023
22. Hyperthermia inhibited cellular function and induced immunogenic cell death of renal cell carcinoma
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Huaqi, Yin, primary, Bingqi, Dong, additional, Yanhui, Zhao, additional, Yongkang, Ma, additional, Shiming, Zhao, additional, Zhenghui, Sun, additional, Zheng, Du, additional, Jiangshan, Peng, additional, and Tiejun, Yang, additional
- Published
- 2023
- Full Text
- View/download PDF
23. Trace Analysis Method Based on UPLC–MS/MS for the Determination of (C2–C18) Per-and Polyfluoroalkyl Substances and Its Application to Tap Water and Bottled Water
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Bingqi Dong, Jing Wu, Yiru Zhuang, Fan Wang, Yueling Zhang, Xiaona Zhang, Hui Zheng, Lixin Yang, and Lin Peng
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Analytical Chemistry - Abstract
As the usage of long-chain perfluoroalkyl and polyfluoroalkyl substances (PFASs) may be gradually restricted, short-chain and even ultra-short-chain PFASs have been widely produced and used, which has put forward new requirements for the simultaneous analysis of the above substances. Using solid phase extraction two-fraction elution and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), an experimental method was established for the simultaneous analysis of ultrashort-chain, short-chain, and long-chain PFASs and the precursor perfluorohexanesulfonamide (FHxSA) in low-concentration water, such as tap water and bottled water. By optimizing the volume of methanol in the first-fraction elution, the concentration of ammonia in the second-fraction elution, and the concentration of ammonium acetate in the mobile phase, the high recovery and low detection limit (0.01-3 ng/L) were obtained. In addition, this method was used to measure nine tap water samples and six bottled water samples for validation, and the results showed that the concentration of PFASs in bottled water was lower than that in tap water. This study first reported the trifluoroacetic acid concentration in bottled water (6.61 ± 9.60 ng/L), which was lower than that in tap water (1712 ± 174 ng/L). The main substances in tap water and bottled water are both ultrashort-chain PFASs (C2-C3), accounting for more than 50%. There are few reports on the simultaneous analysis of ultrashort-chain, short-chain, and long-chain PFASs (C2-C18) and the precursor FHxSA in low-concentration water samples, and the new method can be further developed for different environmental media.
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- 2023
24. Perfluoroalkyl and polyfluoroalkyl substances in cord serum of newborns and their potential factors
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Jingwen Jia, Lihong Duan, Bingqi Dong, Qiuying Dong, Yinping Liu, Wanqin Yu, Lixin Yang, and Hongmei Shi
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Environmental Engineering ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Environmental Chemistry ,General Medicine ,General Chemistry ,Pollution - Abstract
The demonstrated developmental and reproductive toxicity of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), coupled with the increasing production and use of emerging per- and polyfluoroalkyl substances (PFASs) has resulted in progressively higher human exposure levels. This has raised concerns about PFAS exposure levels in the fetus, which is highly susceptible to the potential effects of hazardous environmental chemicals. However, in utero exposure to PFASs and health implications have not been fully characterized in China. To fill this gap, we analyzed 19 PFASs in umbilical cord serum samples (n = 66). Information about the mothers and newborns was obtained through questionnaires. Associations between maternal characteristics and neonatal birth weight and PFAS concentrations were analyzed using nonparametric tests. As results, PFOA was detected in all serum samples. The highest median concentration of PFOS in umbilical serum was 1.092 ng mL
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- 2022
25. In ovo feeding of epidermal growth factor: embryonic expression of intestinal epidermal growth factor receptor and posthatch growth performance and intestinal development in broiler chickens
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Elijah G. Kiarie, Julang Li, Nadeem Akhtar, Chengbo Yang, Emily Kim, Bingqi Dong, and Qianru Hui
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animal structures ,Zygote ,Chick Embryo ,Biology ,broiler ,In ovo ,Metabolism and Nutrition ,Andrology ,03 medical and health sciences ,Epidermal growth factor ,Animals ,Epidermal growth factor receptor ,Receptor ,030304 developmental biology ,0303 health sciences ,Epidermal Growth Factor ,intestinal development and function ,Body Weight ,Embryogenesis ,0402 animal and dairy science ,Broiler ,Embryo ,04 agricultural and veterinary sciences ,General Medicine ,040201 dairy & animal science ,Embryonic stem cell ,in ovo feeding ,ErbB Receptors ,Intestines ,Gene Expression Regulation ,embryonic structures ,biology.protein ,Animal Science and Zoology ,Chickens ,hormones, hormone substitutes, and hormone antagonists - Abstract
We investigated efficacy of in ovo application of epidermal growth factor (EGF) on intestinal expression of EGF receptor (EGFR) during embryogenesis (experiment 1) and posthatch growth performance and gastrointestinal development in broiler chickens (experiment 2). In experiment 1, 450 fertile Ross 708 eggs were allocated to 3 groups (150 eggs/group): 1) control, 2) 160 μg EGF/kg of egg, and 3) 640 μg of EGF/kg of egg. Eggs were candled for live embryos on day 16 and injected with the respective treatment solutions on day 17 and sampled for jejunal tissue from day 17 to hatch for EGFR analyses. There was no effect of EGF (P > 0.05) on EGFR expression on day 17 to 20; however, on day 21, EGF increased (P < 0.05) EGFR expression in EGF birds relative to control birds. In experiment 2, 600 fertile Ross 708 eggs were allocated to 5 treatments: 1) intact, no puncture or injection, 2) punched but not injected, 3) control, no EGF, 4) 80 μg of EGF/kg of egg, and 5) 160 μg of EGF/kg of egg. The eggs were incubated and candled for live embryos on D 19, treated, and subsequently transferred to the hatcher. Upon hatching, chicks were weighed, and 90 chicks per treatment placed in cages (15 birds/cage) and allowed free access to a standard antibiotic-free corn-soybean diet for 21 D. Feed intake and body weight were monitored on a weekly basis. Samples of birds were necropsied on D 0, 7, 14, and 21 for measurements of intestinal weight and jejunal histomorphology and excreta samples taken on D 3 to 5 and 17 to 19 for apparent retention of dry matter. There was no EGF effect (P > 0.05) on any posthatch response criteria. In conclusion, in ovo application of EGF increased EGFR expression but had no effect on posthatch growth performance, DM retention, and intestinal development. The lack of EGF effect on posthatch response was surprising but suggested in ovo application of EGF may not be a viable approach.
- Published
- 2020
26. A laparoscopic radical inguinal lymphadenectomy approach partly preserving great saphenous vein branches can benefit for patients with penile carcinoma
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Bingqi Dong, Weijian Hao, Yongkang Ma, Mingkai Zhu, Tiejun Yang, Zhaohong He, Shiming Zhao, Bao Guan, Chaoshuai Zhu, and Huaqi Yin
- Subjects
Male ,medicine.medical_specialty ,business.industry ,Great saphenous vein ,Carcinoma ,Inguinal lymphadenectomy ,General Medicine ,Femoral Vein ,Surgery ,Postoperative Complications ,Penile Carcinoma ,Medicine ,Humans ,Lymph Node Excision ,Laparoscopy ,Saphenous Vein ,business ,Penile Neoplasms ,Retrospective Studies - Abstract
Background Inguinal lymphadenectomy (iLAD) is effective for penile carcinoma treatment, but usually results in many complications. This study aims to clinically evaluate the feasibility and clinical significance of a laparoscopic radical iLAD approach partly preserving great saphenous vein branches for penile carcinoma patients. Methods A total of 48 patients with penile cancer who underwent laparoscopic radical iLAD with retention of the great saphenous vein in Henan Cancer Hospital from 2012 Jan to 2020 Dec were included in this study. Sixteen penile carcinoma patients who underwent laparoscopic radical iLAD preserving parts of superficial branches of the great saphenous vein were identified as the sparing group, and the matched 32 patients who incised those branches were identified as control group. This new procedure was performed by laparoscopy, preserving parts of superficial branches of the great saphenous vein, superficial lateral and medial femoral veins. Clinicopathological features and perioperative variables were recorded. Postoperative complications, including skin flap necrosis, lymphorrhagia, and lower extremity edema were analyzed retrospectively. Results We found that the operative time of the sparing group is significantly longer than the control group (p = 0.011). There was no statistical difference in intraoperative blood loss, the lymph node number per side, average time to remove the drainage tube and postoperative hospital stay between the two groups. Compared to the control group, the sparing group showed a significantly decreased incidence of lower extremity edema (p = 0.018). The preservation of parts of superficial branches of the great saphenous vein was mainly decreased the incidence of edema below ankle (p = 0.034). Conclusions This study demonstrated that the iLAD with preserving parts of superficial branches of the great saphenous vein, with a decreased incidence of postoperative complications, is a safe and feasible approach for penile cancer.
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- 2021
27. Capsaicin Attenuates Lipopolysaccharide-Induced Inflammation and Barrier Dysfunction in Intestinal Porcine Epithelial Cell Line-J2
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Changqing Zhu, Chengbo Yang, Marissa Friesen, Shangxi Liu, Xiaoya Zhao, and Bingqi Dong
- Subjects
Lipopolysaccharide ,Tight junction ,Physiology ,LPS induction ,Inflammation ,Pharmacology ,capsaicin ,IPEC-J2 ,Proinflammatory cytokine ,chemistry.chemical_compound ,TLR4/NF-κB pathway ,chemistry ,inflammation ,Capsaicin ,Physiology (medical) ,medicine ,TLR4 ,QP1-981 ,lipids (amino acids, peptides, and proteins) ,medicine.symptom ,Signal transduction ,Receptor ,Original Research ,gut integrity - Abstract
Capsaicin is a spicy, highly pungent, colorless, vanilloid compound found in chili peppers with anti-inflammatory, antioxidant, anti-cancer, and analgesic properties. However, the protective effects of capsaicin on the pig intestine during inflammation are yet to be explored. This study investigated the effects of capsaicin on the gut inflammatory response, intestinal epithelial integrity, and gene expression level of nutrient transporters in a model of lipopolysaccharide (LPS)-induced inflammation in non-differentiated intestinal porcine epithelial cell line-J2 (IPEC-J2). The results showed that the pre-treatment of cells with capsaicin (100 μM) significantly decreased the gene expression and secretion of proinflammatory cytokines induced by LPS through Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. In addition, pre-treatment of cells with capsaicin also increased both gene and protein abundance of tight junction proteins. Furthermore, pre-treatment cells with capsaicin significantly increased trans-epithelial electrical resistance (TEER) and decreased permeability of fluorescein isothiocyanate-dextran (FD4) from the apical side to the basolateral side compared with the control (P < 0.05). Additionally, pre-treatment of cells with capsaicin upregulated the mRNA abundance of nutrients transporters such as Na+/glucose cotransporter 1 (SGLT1). These results suggested that capsaicin could attenuate LPS-induced inflammation response through TLR4/NF-κB pathway and improve barrier integrity and glucose absorption.
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- 2021
28. Birth Weight and Nutrient Restriction Affect Jejunal Enzyme Activity and Gene Markers for Nutrient Transport and Intestinal Function in Piglets
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Chengbo Yang, Daniel A Columbus, Josiane C. Panisson, Michael O Wellington, Bingqi Dong, Lucas A Rodrigues, and Qiao Li
- Subjects
medicine.medical_specialty ,Birth weight ,Veterinary medicine ,Article ,03 medical and health sciences ,Internal medicine ,Gene expression ,SF600-1100 ,medicine ,Weaning ,low birth weight ,intestinal development ,030304 developmental biology ,restricted nutrition ,2. Zero hunger ,0303 health sciences ,General Veterinary ,biology ,Peptide transporter 1 ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,normal birth weight ,Glutamine ,Low birth weight ,Endocrinology ,QL1-991 ,biology.protein ,gene expression ,Alkaline phosphatase ,Animal Science and Zoology ,neonatal piglets ,medicine.symptom ,Maltase ,Zoology ,normal nutrition - Abstract
Significant variation in the birth weight of piglets has arisen due to increased sow prolificacy. Intestinal development and function may be affected by birth weight. Low birth weight (LBW) pigs may also have reduced feed intake, leading to further impairment of intestinal development. The objective of this study was to examine the intestinal development pattern of LBW and normal birth weight (NBW) piglets with normal nutrition (NN) or restricted nutrition (RN) in the pre-weaning period. Jejunal intestinal samples were analyzed for target gene expression and enzyme activity at d 28 (weaning) and d 56 (post-weaning). At d 28, excitatory amino acid transporter (EAAC1) and sodium-dependent neutral amino acid transporter (B0AT1) were downregulated in LBW compared to NBW pigs (p <, 0.05). On d 56, B0AT1 and ASCT2 (glutamine transporter) were downregulated in RN compared to NN pigs (p <, 0.05), regardless of birth weight. Peptide transporter 1 (PepT1) expression was downregulated in LBW compared to NBW pigs at 28 d (p <, 0.05), with no effects of treatments at 56 d. Sodium-glucose transporter-1 (SGLT1) was upregulated in NBW-NN compared to LBW-NN pigs (p <, 0.05) at 28 d. Alkaline phosphatase (ALP) was upregulated in LBW-RN at d 28. At d 56, claudin-3 (CLDN-3) and Zonular occludin-1 (ZO-1) were upregulated in NN compared to RN pigs (p <, 0.05). There were no treatment effects on ALP, maltase, or sucrase activity at 28 d. However, at 56 d, ALP was upregulated in NBW-NN pigs while sucrase activity was upregulated in NN pigs (p <, 0.05). The results demonstrate differences in jejunal gene expression associated with birth weight, with reduced gene expression of amino acid transporters (PepT1, EAAC1, B0AT1) in LBW compared to NBW pigs (p <, 0.05). While neonatal nutrient restriction had minimal effects at 28 d and d 56 for tight junction protein transcript abundance, neutral amino acid transporter abundance was upregulated in NN pigs compared to RN piglets (p <, 0.05).
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- 2021
29. Identification of a Prognostic Signature Associated With the Homeobox Gene Family for Bladder Cancer
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Yongkang Ma, Jinbo Song, Wenping Song, Ding Li, Shiming Zhao, Bingqi Dong, Jiaming Liang, Tiejun Yang, and Mingkai Zhu
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,QH301-705.5 ,medicine.medical_treatment ,Biology ,medicine.disease_cause ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,homeobox gene family ,Internal medicine ,medicine ,Molecular Biosciences ,prognostic signature ,KEGG ,Biology (General) ,Gene ,Molecular Biology ,Original Research ,Bladder cancer ,biomarkers ,Immunotherapy ,medicine.disease ,TSHZ3 ,030104 developmental biology ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,Homeobox ,bladder cancer ,immunotherapy ,Carcinogenesis - Abstract
Background: Bladder cancer (BLCA) is a common malignant tumor of the genitourinary system, and there is a lack of specific, reliable, and non-invasive tumor biomarker tests for diagnosis and prognosis evaluation. Homeobox genes play a vital role in BLCA tumorigenesis and development, but few studies have focused on the prognostic value of homeobox genes in BLCA. In this study, we aim to develop a prognostic signature associated with the homeobox gene family for BLCA.Methods: The RNA sequencing data, clinical data, and probe annotation files of BLCA patients were downloaded from the Gene Expression Omnibus database and the University of California, Santa Cruz (UCSC), Xena Browser. First, differentially expressed homeobox gene screening between tumor and normal samples was performed using the “limma” and robust rank aggregation (RRA) methods. The mutation data were obtained with the “TCGAmutation” package and visualized with the “maftools” package. Kaplan–Meier curves were plotted with the “survminer” package. Then, a signature was constructed by logistic regression analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using “clusterProfiler.” Furthermore, the infiltration level of each immune cell type was estimated using the single-sample gene set enrichment analysis (ssGSEA) algorithm. Finally, the performance of the signature was evaluated by receiver-operating characteristic (ROC) curve and calibration curve analyses.Results: Six genes were selected to construct this prognostic model: TSHZ3, ZFHX4, ZEB2, MEIS1, ISL1, and HOXC4. We divided the BLCA cohort into high- and low-risk groups based on the median risk score calculated with the novel signature. The overall survival (OS) rate of the high-risk group was significantly lower than that of the low-risk group. The infiltration levels of almost all immune cells were significantly higher in the high-risk group than in the low-risk group. The average risk score for the group that responded to immunotherapy was significantly lower than that of the group that did not.Conclusion: We constructed a risk prediction signature with six homeobox genes, which showed good accuracy and consistency in predicting the patient’s prognosis and response to immunotherapy. Therefore, this signature can be a potential biomarker and treatment target for BLCA patients.
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- 2021
30. Clinical significance of circulating tumour cells and Ki-67 in renal cell carcinoma
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Bingqi Dong, Xiaofeng Guo, Yongkang Ma, Jinbo Song, Tiejun Yang, Zhe Yu, Mingkai Zhu, and Shiming Zhao
- Subjects
medicine.medical_specialty ,RD1-811 ,medicine.medical_treatment ,Urology ,Renal cell carcinoma ,Surgical oncology ,Biomarkers, Tumor ,medicine ,Humans ,Clinical significance ,neoplasms ,Carcinoma, Renal Cell ,RC254-282 ,Perioperative period ,biology ,business.industry ,Genitourinary system ,Research ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Perioperative ,Neoplastic Cells, Circulating ,Prognosis ,medicine.disease ,Kidney Neoplasms ,Nephrectomy ,Circulating tumour microemboli (CTM) ,Ki-67 Antigen ,Oncology ,Ki-67 ,biology.protein ,Immunohistochemistry ,Surgery ,business ,Circulating tumour cells (CTCs) - Abstract
Background Renal cell carcinoma (RCC) is a common malignant tumour of the genitourinary system. We aimed to analyse the potential value of metastasis-related biomarkers, circulating tumour cells (CTCs) and the proliferative marker Ki-67 in the diagnosis of RCC. Methods Data from 24 laparoscopic radical nephrectomies (RNs) and 17 laparoscopic partial nephrectomies (PNs) were collected in 2018. The numbers and positive rates of CTCs and circulating tumour microemboli (CTM) in the peripheral blood were obtained at three different time points: just before surgery, immediately after surgery and 1 week after surgery. Ki-67 protein expression was evaluated in the RCC tissue by immunohistochemistry. Results Except for the statistically significant association between the preoperative CTC counts and tumour size, no association between the number and positive rate of perioperative CTCs and clinicopathological features was found. The CTC counts gradually decreased during the perioperative period, and at 1 week after surgery, they were significantly lower than those before surgery. High Ki-67 expression was significantly positively correlated with preoperative CTC counts. In addition, Ki-67 expression was higher in the high CTC group (≥ 5 CTCs). Conclusion Our results suggest that surgical nephrectomy is associated with a decrease in CTC counts in RCC patients. CTCs can act as a potential biomarker for the diagnosis and prognosis of RCC. A careful and sufficient long-term follow-up is needed for patients with high preoperative CTC counts.
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- 2021
31. Additional file 2 of Clinical significance of circulating tumour cells and Ki-67 in renal cell carcinoma
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Jinbo Song, Yu, Zhe, Bingqi Dong, Mingkai Zhu, Xiaofeng Guo, Yongkang Ma, Shiming Zhao, and Yang, Tiejun
- Subjects
neoplasms - Abstract
Additional file 2: Supplementary table 2. The correlation of CTC counts with the pathological features of patients one week after surgery
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- 2021
- Full Text
- View/download PDF
32. Evaluation of the reporting quality of guidelines for gastric cancer using the RIGHT checklist
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Hanqiong Zhou, Zhen He, Yingxi Wu, Kristina A. Matkowskyj, Xuan Wu, Khaldoun Almhanna, Jing Han, Haiyang Chen, Qiming Wang, Aslam Ejaz, Ding Li, Bingqi Dong, and Yanfang Ma
- Subjects
medicine.medical_specialty ,business.industry ,media_common.quotation_subject ,International standard ,MEDLINE ,Declaration ,General Medicine ,Guideline ,Checklist ,Family medicine ,Health care ,medicine ,Original Article ,Quality (business) ,business ,Quality assurance ,media_common - Abstract
Background Gastric cancer is the fifth most common type of cancer globally. We aimed to evaluate the reporting quality of clinical practice guidelines in the field of gastric cancer. Methods We searched Medline (via PubMed), China Biology Medicine, Chinese National Knowledge Infrastructure and WanFang databases and the websites of the main guideline development organizations from 2018 to 2020 for guidelines on gastric cancer. Data were extracted and the reporting quality evaluated by two researchers independently using the Reporting Items for Practice Guidelines in Healthcare (RIGHT) checklist. We assessed the compliance of the guidelines to each of the 35 items of RIGHT and summarized the reporting proportions of the seven domains of RIGHT. Results Eighteen guidelines were included. The mean proportion of appropriately reported RIGHT items was 52.4%. Among the seven domains of the RIGHT checklist, Basic information had the highest reporting rate (78.7%), and Review and quality assurance domain the lowest rate (16.7%). The domains Evidence (40.0%), Funding and declaration and management of interests (43.1%), and Other information (31.5%) had also reporting rates below 50%. Two RIGHT items (17 and 19b) were not reported by any of the guidelines. Conclusions The reporting quality of gastric cancer guidelines published in the years 2018-2020 was suboptimal, especially regarding the reporting of review, quality assurance and evidence. Guideline developers should pay attention on rigorous reporting following international standard to improve the quality of guidelines.
- Published
- 2021
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