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Background: Clinical studies comparing retention rates of pit and fissure sealants placed under Isolite, rubber dam, and cotton roll combined have not been conducted until now. Aim: To evaluate the retention rate of pit and fissure sealants (PFS) placed under three different isolation techniques (Isolite system [IS], rubber dam isolation [RDI], and cotton roll isolation [CRI]). Design: One hundred and forty‐four teeth from 48 children aged 6–15 years attending paediatric dental clinics at King Saud University in Saudi Arabia were randomized to receive three PFS using three isolation techniques. The children that met the inclusion criteria were randomized by a simple block random allocation method. All PFS were placed by an operator and evaluated by a blinded evaluator. The evaluation scores were recorded at baseline and followed up over a period of 12–22 months. Results: The children's mean age was 8.58 ± 1.93 years. Seven patients were lost to follow‐up. A total of 123 teeth were clinically evaluated; of these teeth, 22% had completely retained sealants, whereas approximately 66% had a partial loss of sealants, and approximately 12% had a complete loss of sealants. There were, however, no significant differences between the three isolation techniques on the retention rate of pit and fissure sealant. Conclusion: The types of isolation had no impact on the retention rate of pit and fissure sealant. [ABSTRACT FROM AUTHOR]

Aim: The aim of the present study was to evaluate the effect of aging on the microhardness score [Vickers hardness number (VHN)] of different resin-based fluoride-releasing sealants compared to non-fluoride resin-based sealants., Materials and Methods: A total of 48 extracted sound molars that were sectioned mesiodistally were used. In the current study, four types of sealants were compared: Group A, a resin-based non-fluoride-releasing pit and fissure sealant (Eco-S sealant) served as a control. Groups B, C, and D received resin-based fluoride-releasing pit and fissure sealants Helioseal F, Fissurit F, and Embrace ™ WetBond, respectively. Subsequently, each group was further divided into immediate and aged subgroups. The samples had been evaluated regarding their microhardness using a pyramidal diamond indenter of a Vickers hardness test at two-time intervals: Immediately and after the aging process through thermocycling., Results: There were no statistically significant differences between mean VHN and material types ( p = 0.72). Aging appeared to significantly increase the mean VHN ( p = 0.001). The interaction model between material type and time factor showed that the effect of aging differs by the material type, where the VHN of the Embrace ™ group increased significantly after aging from 24.33 ± 5.60 to 31.70 ± 3.59 ( p = 0.001)., Conclusion: While there were no significant differences in the microhardness of commonly used fluoride-releasing fissure sealants, time appears to significantly increase the mean microhardness score (VHN), especially in the Embrace ™ group., Clinical Significance: Embrace ™ WetBond fissure sealant showed a significant improvement in the mean microhardness score (VHN) with time. However, clinical studies with long-term follow-up are needed to confirm our results.