403 results on '"Biofertilization"'
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2. Effect of Seed Priming and Biofertilization Treatments on Vegetative Growth Characters of Soybean under Water Stress Conditions.
- Author
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Seadh, S. E., El-Moneam, M. A. Abd, El-Saidy, Aml E. A., Attia, A. N. E., and Ghozzy, A. A. M. B.
- Subjects
IRRIGATION water ,CALCIUM chloride ,WATER purification ,HUMIC acid ,MYCORRHIZAS - Abstract
Copyright of Journal of Plant Production is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
- Full Text
- View/download PDF
3. Optimizing an Ultisol of marginal land for soybean cultivation through inorganic fertilizer substitution with Tithonia compost and biochar ameliorant
- Author
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Ardiyaningsih Puji Lestari, Zulkarnain, Adriani, Mapegau, Sosiawan Nusifera, and Dede Martino
- Subjects
ameliorants ,biofertilization ,soybean ,substitution ,ultisol ,Environmental effects of industries and plants ,TD194-195 - Abstract
Soybean has a strategic role as a food commodity in Jambi, Indonesia, but until now, it has still not been self-sufficient. There is still an opportunity to increase soybean productivity in Jambi by 35-49.6% by optimizing the use of marginal, dominated by Ultisol, which has low inherent soil fertility. One of the efforts that can be made to improve soil fertility for cultivating soybean plants in Jambi is to utilize compost and biochar as soil ameliorants to substitute for inorganic fertilizer. This study aimed to assess the effect of compost and biochar ameliorant as substitutes for inorganic fertilizer on the growth and yield of soybean plants grown on an Ultisol of Jambi. The experiment was arranged in a simple randomized block design with the combination of the percentage of inorganic fertilizer with compost and biochar ameliorant (100+0, 75+25, 50+50, 25+75, and 0+100) as the treatment factors. The observed variables were plant height, number of branches, plant nitrogen content, rate of symbiotically fixed nitrogen, weight of 100 seeds, and yield. The results showed that providing compost and biochar ameliorants can replace the role of inorganic fertilizer, and providing 100% compost and biochar ameliorant and a combination of 25% inorganic fertilizer and 75% compost biochar ameliorant can provide the highest growth and yield in soybean plants planted in Ultisol dry land.
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- 2024
- Full Text
- View/download PDF
4. Technical and Economic Assessment of Tomato Cultivation Through a Macro-Tunnel Production System with the Application of Gluconacetobacter diazotrophicus.
- Author
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Ceballos-Aguirre, Nelson, Hurtado-Salazar, Alejandro, Restrepo, Gloria M., Sánchez, Óscar J., Hernández, María C., and Montoya, Mauricio
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TOMATO farming ,NITROGEN fertilizers ,NITROGEN fixation ,U.S. dollar ,ECONOMIC indicators - Abstract
Bacterial inoculants hold promise for enhancing the sustainability and profitability of tomato cultivation in macro-tunnel systems. This study aimed to evaluate the technical and economic viability of applying Gluconacetobacter diazotrophicus to tomato production. The separate addition of native G. diazotrophicus GIBI025 and GIBI029 isolates and a commercial inoculant containing Azotobacter chrococcum and Azospirillium sp. was evaluated at a rate of 1 × 10
8 CFU·mL−1 without nitrogen addition. Conventional fertilization treatment with no bacteria added and 100%-nitrogen fertilization relative to crop requirements (added as MAP and urea) was also assessed. The treatments were evaluated within the macro-tunnel production system. The experiment utilized a completely randomized block design with four replications per treatment, and each experimental unit consisted of 20 plants. The yield (kg·ha−1 ) was calculated and economic assessment was performed. The results show that native G. diazotrophicus isolates in tomato cultivation under the macro-tunnel production system improved its economic viability, achieving yields up to 95,501 kg·ha−1 without the addition of nitrogenous fertilizers. This research reveals benefit–cost ratios achieving 1.57 and net incomes reaching 16,707 US dollars per hectare. This work demonstrated that the native isolates assessed may be used in the pursuit of more integrated, sustainable, and competitive cultural practices. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
5. Optimizing an Ultisol of marginal land for soybean cultivation through inorganic fertilizer substitution with Tithonia compost and biochar ameliorant.
- Author
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Lestari, Ardiyaningsih Puji, Zulkarnain, Adriani, Mapegau, Nusifera, Sosiawan, and Martino, Dede
- Subjects
SOYBEAN ,FOOD ,SOIL fertility ,FERTILIZERS ,NITROGEN content of sewage - Abstract
Soybean has a strategic role as a food commodity in Jambi, Indonesia, but until now, it has still not been self-sufficient. There is still an opportunity to increase soybean productivity in Jambi by 35-49.6% by optimizing the use of marginal, dominated by Ultisol, which has low inherent soil fertility. One of the efforts that can be made to improve soil fertility for cultivating soybean plants in Jambi is to utilize compost and biochar as soil ameliorants to substitute for inorganic fertilizer. This study aimed to assess the effect of compost and biochar ameliorant as substitutes for inorganic fertilizer on the growth and yield of soybean plants grown on an Ultisol of Jambi. The experiment was arranged in a simple randomized block design with the combination of the percentage of inorganic fertilizer with compost and biochar ameliorant (100+0, 75+25, 50+50, 25+75, and 0+100) as the treatment factors. The observed variables were plant height, number of branches, plant nitrogen content, rate of symbiotically fixed nitrogen, weight of 100 seeds, and yield. The results showed that providing compost and biochar ameliorants can replace the role of inorganic fertilizer, and providing 100% compost and biochar ameliorant and a combination of 25% inorganic fertilizer and 75% compost biochar ameliorant can provide the highest growth and yield in soybean plants planted in Ultisol dry land. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Biofertilization potential of products from biotransformation of organic waste in Morocco: comparing between aerobic and anaerobic mode.
- Author
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Foughal, Tarik, Doublali, Fatima Ezzahra, Ozi, Fatima Zahra, Hadidi, Meryem, Louanjli, Fatima Azzahra, Bahlaouan, Bouchaib, Antri, Said El, and Boutaleb, Nadia
- Abstract
Morocco's management of agri-food waste presents serious environmental problems that threaten natural environment and human health. It has become crucial to use more effective technologies, like composting or anaerobic digestion, for recycling food waste in order to address this problem. Such technologies must to not only provide solutions but also provide beneficial outcomes. Therefore, conducting comparative studies to identify the most effective technologies is crucial. The present study aims to examine the potential of converting this waste into a high-quality biofertilizer by comparing between the aerobic and anaerobic bioconversion with the use of a fungal inoculum in order to enhance the quality and time of the biological process. Additionally, the study examines the effect of using a single type of waste or a variety of wastes on the quality of the biofertilizer produced. A fungus known for its capacity to accelerate biotransformation, Aspergillus niger, was added to a mixture of fish, vegetable, and poultry wastes. In a 20-day process, the impact of waste formulation on nutritional and hygienic potential was assessed based on biofertilization standards. According to the results, a combination of all three waste types with a tiny excess of poultry waste was the best mixture for biofertilization. Regardless of the bioconversion mechanism employed, this mixture was proven to be effective and secure. Anaerobic digestion product had a higher potential for biofertilization than aerobic biotransformation product, according to fertilization tests on tomato crops. Therefore, choosing the most appropriate method of bioconversion is essential to produce a high-quality biofertilizer that can enhance plant growth. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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7. Response Superior Grape Yield and Quality to Organic and Inorganic Fertilization.
- Author
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Wassel, A. M. M., Hassan, E. A., and Ahmed, Soad E. A.
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GRAPE yields ,GRAPE quality ,GRAPES ,COMPOSTING ,CLIMBING plants ,BERRIES - Abstract
Copyright of Scientific Journal of Agricultural Sciences (SJAS) is the property of Beni Suef University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
- Full Text
- View/download PDF
8. Applications of the Microalgae Chlamydomonas and Its Bacterial Consortia in Detoxification and Bioproduction.
- Author
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Torres, María J., Bellido-Pedraza, Carmen M., and Llamas, Angel
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CONSORTIA , *PHOSPHORUS metabolism , *WASTE recycling , *BIOTECHNOLOGY , *CROP yields - Abstract
The wide metabolic diversity of microalgae, their fast growth rates, and low-cost production make these organisms highly promising resources for a variety of biotechnological applications, addressing critical needs in industry, agriculture, and medicine. The use of microalgae in consortia with bacteria is proving valuable in several areas of biotechnology, including the treatment of various types of wastewater, the production of biofertilizers, and the extraction of various products from their biomass. The monoculture of the microalga Chlamydomonas has been a prominent research model for many years and has been extensively used in the study of photosynthesis, sulphur and phosphorus metabolism, nitrogen metabolism, respiration, and flagellar synthesis, among others. Recent research has increasingly recognised the potential of Chlamydomonas–bacteria consortia as a biotechnological tool for various applications. The detoxification of wastewater using Chlamydomonas and its bacterial consortia offers significant potential for sustainable reduction of contaminants, while facilitating resource recovery and the valorisation of microalgal biomass. The use of Chlamydomonas and its bacterial consortia as biofertilizers can offer several benefits, such as increasing crop yields, protecting crops, maintaining soil fertility and stability, contributing to CO2 mitigation, and contributing to sustainable agricultural practises. Chlamydomonas–bacterial consortia play an important role in the production of high-value products, particularly in the production of biofuels and the enhancement of H2 production. This review aims to provide a comprehensive understanding of the potential of Chlamydomonas monoculture and its bacterial consortia to identify current applications and to propose new research and development directions to maximise their potential. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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9. Plant-Growth-Promoting Rhizobacteria Improve Seeds Germination and Growth of Argania spinosa.
- Author
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Chabbi, Naima, Chafiki, Salahddine, Telmoudi, Maryem, Labbassi, Said, Bouharroud, Rachid, Tahiri, Abdelghani, Mentag, Rachid, El Amri, Majda, Bendiab, Khadija, Hsissou, Driss, Mimouni, Abdelaziz, Ait Aabd, Naima, and Qessaoui, Redouan
- Subjects
PLANT growth-promoting rhizobacteria ,ROOT development ,SUSTAINABLE agriculture ,ALTERNATIVE agriculture ,INDOLEACETIC acid - Abstract
Argania spinosa is among the most important species of the Moroccan forest in terms of ecological, environmental, and socio-economic aspects. However, it faces a delicate balance between regeneration and degradation in its natural habitat. Hence, the efforts to preserve and regenerate argan forests are crucial for biodiversity, soil quality, and local livelihoods, yet they face challenges like overgrazing and climate change. Sustainable management practices, including reforestation and community engagement, are vital for mitigating degradation. Similarly, exploiting the argan tree's rhizosphere can enhance soil quality by leveraging its rich microbial diversity. This approach not only improves crop growth but also maintains ecosystem balance, ultimately benefiting both agriculture and the environment. This enrichment can be achieved by different factors: mycorrhizae, plant extracts, algae extracts, and plant growth-promoting rhizobacteria (PGPR). The benefits provided by PGPR may include increased nutrient availability, phytohormone production, shoot, root development, protection against several plant pathogens, and disease reduction. In this study, the effect of rhizobacteria isolated from the Agran rhizosphere was evaluated on germination percentage and radicle length for Argania spinosa in vitro tests, growth, collar diameter, and branching number under greenhouse conditions. One hundred and twenty (120) bacteria were isolated from the argan rhizosphere and evaluated for their capacity for phosphate solubilization and indole acetic acid production. The results showed that 52 isolates could solubilize phosphorus, with the diameters of the solubilization halos varying from 0.56 ± 0.14 to 2.9 ± 0.08 cm. Among 52 isolates, 25 were found to be positive for indole acetic acid production. These 25 isolates were first tested on maize growth to select the most performant ones. The results showed that 14 isolates from 25 tested stimulated maize growth significantly, and 3 of them by 28% (CN005, CN006, and CN009) compared to the control. Eight isolates (CN005, CN006, CN004, CN007, CN008, CN009, CN010, and CN011) that showed plant growth of more than 19% were selected to evaluate their effect on argan germination rate and radicle length and were subjected to DNA extraction and conventional Sanger sequencing. The 8 selected isolates were identified as: Brevundimonas naejangsanensis sp2, Alcaligenes faecalis, Brevundimonas naejangsanensis sp3, Brevundimonas naejangsanensis sp4, Leucobacter aridicollis sp1, Leucobacter aridicollis sp2, Brevundimonas naejangsanensis sp1, and Staphylococcus saprophyticus. The results showed that Leucobacter aridicollis sp2 significantly increased the germination rate by 95.83%, and the radicle length with a value of 2.71 cm compared to the control (1.60 cm), followed by Brevundimonas naejangsanensis sp3 and Leucobacter aridicollis sp1 (2.42 cm and 2.11 cm, respectively). Under greenhouse conditions, the results showed that the height growth increased significantly for Leucobacter aridicollis sp1 (42.07%) and Leucobacter aridicollis sp2 (39.99%). The isolates Brevundimonas naejangsanensis sp3 and Leucobacter aridicollis sp1 increased the gain of collar diameter by 41.56 and 41.21%, respectively, followed by Leucobacter aridicollis sp2 and Staphyloccocus saprophyticus (38.68 and 22.79%). Leucobacter aridicollis sp1 increased the ramification number per plant to 12 compared to the control, which had 6 ramifications per plant. The use of these isolates represents a viable alternative in sustainable agriculture by improving the germination rate and root development of the argan tree, as well as its development, while increasing the availability of nutrients in the soil and consequently improving fertilization. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. Impact of Organic, NPK and Biofertilization on Yield of Cowpea Cultivars Under Arid Land Conditions
- Author
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Dalia Soliman
- Subjects
biofertilization ,cowpea ,farmyard manure ,chicken manure ,organic fertilization ,npk fertilization ,Agriculture - Abstract
This study was conducted to investigate the effects of organic, NPK, and biofertilizers on the yield of two cowpea varieties grown under arid land conditions. Two cultivars of cowpea (Karim-7 and Dokki-331) were evaluated using different fertilizer types. The fertilizers examined were organic (farmyard manure (FYM) and chicken manure (CHM)) and biofertilizers (effective microorganisms (EM1) and technology of smart fertilizer (TS)) in addition to NPK treatments (NPK (50 kg/fed), NPK (100 kg/fed), EM1 (15 m3.fed-1) + NPK (50 kg/fed), TS (15 m3.fed-1) + NPK (50 kg/fed). A randomized complete block design (RCBD) with 16 treatments and three replications was used to set up the experiment. The measured yield parameters were seed number/plant, pod length, seed number/pod, pod number/plant, dry yield/plant, dry yield/m2, bio yield, pod weight, 100-seed weight, and grain yield. The results cleared that the cultivar dokki-331 under EM1 biofertilizer + NPK (50 kg/fed) combination treatment was the superior practice for increasing all studied traits.
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- 2024
- Full Text
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11. Field inoculation by arbuscular mycorrhizal fungi with contrasting life-history strategies differently affects tomato nutrient uptake and residue decomposition dynamics.
- Author
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Arcidiacono, Myriam, Pellegrino, Elisa, Nuti, Marco, and Ercoli, Laura
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VESICULAR-arbuscular mycorrhizas , *LIFE history theory , *NUTRIENT uptake , *TOMATOES , *ORGANIC farming , *FUNGAL colonies - Abstract
Purpose: Plant microbial biostimulants, such as arbuscular mycorrhizal fungi (AMF), enhance nutrient concentration in fruits, including tomato. However, field studies on tomato AMF inoculation are scarce. AMF species belonging to Gigasporaceae and Glomeraceae families known to vary in life-history strategies may determine differential effects on plant nutrient benefits and residue decomposition. Despite this, the effect of different life-history strategies on nutrient acquisition of tomato fruits has not been investigated yet. Methods: We studied the effect of inoculation of two tomato varieties with four AMF species belonging to Glomeraceae and Gigasporaceae. Fungal colonization, yield, fruit nutrient concentration, litter decomposition, and bacterial and fungal abundances in soil were assessed in the field under organic agriculture. Results: Overall Gigasporaceae promoted the concentration of nutrients in tomato fruits compared to Glomeraceae. A variability in AM fungal colonization and fruit nutrient concentration was detected within Glomeraceae. Scutellospora pellucida increased the yield (+ 27%) of var. Rio Grande with respect to Gigaspora gigantea. In var. Rio Grande, inoculation with Funneliformis mosseae did not change litter decomposition as compared to non-inoculated controls, whereas it was lower than in Sclerocystis sinuosa and Gigasporaceae species, which showed the highest decomposition rates. AMF inoculation promoted soil total bacterial and fungal abundance and fungal:bacterial (F:B) ratio compared to controls, and members of Gigasporaceae had the highest F:B ratio. Conclusion: These findings pointed at the inclusion of AM fungal life-history strategy within the selection criteria for the development of biofertilizers able to enhance the nutritional value of vegetables under organic farming systems. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
- View/download PDF
12. Foliar diagnosis of common arrowroot propagated by different forms and fertilized with biofertilizer.
- Author
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Lima, Ana K. B., da Costa, Rafael S., Borges, Francisca R. M., Dias, Thiago J., Mamede, George L., Amorim, Aiala V., Silva, Mateus G. J. B., de C. Lourencio, Rosaliny, de Melo Junior, Madson F., and Marinho, Albanise B.
- Subjects
FOLIAR diagnosis ,PLANT nutrition ,PLANT stems ,EDIBLE plants ,BLOCK designs - Abstract
Copyright of Revista Brasileira de Engenharia Agricola e Ambiental - Agriambi is the property of Revista Brasileira de Engenharia Agricola e Ambiental and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
- Full Text
- View/download PDF
13. Exploring the Role of Debaryomyces hansenii as Biofertilizer in Iron-Deficient Environments to Enhance Plant Nutrition and Crop Production Sustainability.
- Author
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Sevillano-Caño, Jesús, García, María José, Córdoba-Galván, Clara, Luque-Cruz, Carmen, Agustí-Brisach, Carlos, Lucena, Carlos, Ramos, José, Pérez-Vicente, Rafael, and Romera, Francisco Javier
- Subjects
- *
PLANT nutrition , *CROPS , *FLOWER development , *AGRICULTURE , *AGRICULTURAL productivity , *CUCUMBERS - Abstract
The European "Green Deal" policies are shifting toward more sustainable and environmentally conscious agricultural practices, reducing the use of chemical fertilizer and pesticides. This implies exploring alternative strategies. One promising alternative to improve plant nutrition and reinforce plant defenses is the use of beneficial microorganisms in the rhizosphere, such as "Plant-growth-promoting rhizobacteria and fungi". Despite the great abundance of iron (Fe) in the Earth's crust, its poor solubility in calcareous soil makes Fe deficiency a major agricultural issue worldwide. Among plant promoting microorganisms, the yeast Debaryomyces hansenii has been very recently incorporated, for its ability to induce morphological and physiological key responses to Fe deficiency in plants, under hydroponic culture conditions. The present work takes it a step further and explores the potential of D. hansenii to improve plant nutrition and stimulate growth in cucumber plants grown in calcareous soil, where ferric chlorosis is common. Additionally, the study examines D. hansenii's ability to induce systemic resistance (ISR) through a comparative relative expression study by qRT-PCR of ethylene (ET) biosynthesis (ACO1), or ET signaling (EIN2 and EIN3), and salicylic acid (SA) biosynthesis (PAL)-related genes. The results mark a significant milestone since D. hansenii not only enhances nutrient uptake and stimulates plant growth and flower development but could also amplify induced systemic resistance (ISR). Although there is still much work ahead, these findings make D. hansenii a promising candidate to be used for sustainable and environmentally friendly integrated crop management. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
14. Impact of Organic, NPK and Biofertilization on Yield of Cowpea Cultivars Under Arid Land Conditions.
- Author
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El-Atty Soliaman, Dalia Abd
- Subjects
- *
BIOFERTILIZERS , *FARM manure , *POULTRY manure , *SOIL fertility management , *SUSTAINABLE agriculture - Abstract
This study was conducted to investigate the effects of organic, NPK and biofertilizers on the yield of two cowpea varieties grown under arid land conditions. Two cultivars of cowpea (Karim-7 and Dokki-331) were evaluated using different fertilizer types. The fertilizers examined were organic (farmyard manure (FYM) and chicken manure (CHM)) and biofertilizers (effective microorganisms (EM1) and technology of smart fertilizer (TS)) in addition to NPK treatments (NPK (50 kg/fed), NPK (100 kg/fed), EM1 (15 m³ .fed-1 ) + NPK (50 kg/fed), TS (15 m³ .fed-1 ) + NPK (50 kg/fed). A randomized complete block design (RCBD) with 16 treatments and three replications was used to set up the experiment. The measured yield parameters were seed number/plant, pod length, seed number/pod, pod number/plant, dry yield/plant, dry yield/m2, bio yield, pod weight, 100-seed weight, and grain yield. The results cleared that the cultivar dokki-331 under EM1 biofertilizer + NPK (50 kg/fed) combination treatment was the superior practice for increasing all studied traits. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
15. Phosphate solubilizing Pseudomonas and Bacillus combined with rock phosphates promoting tomato growth and reducing bacterial canker disease.
- Author
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Bakki, Mohamed, Banane, Badra, Marhane, Omaima, Esmaeel, Qassim, Hatimi, Abdelhakim, Barka, Essaid Ait, Azim, Khalid, and Bouizgarne, Brahim
- Subjects
PHOSPHATE rock ,TOMATOES ,BIOPESTICIDES ,BACTERIAL diseases ,BACILLUS (Bacteria) ,SUSTAINABLE agriculture ,PSEUDOMONAS - Abstract
Nowadays, sustainable agriculture approaches are based on the use of biofertilizers and biopesticides. Tomato (Solanum lycopersicum L.) rhizosphere could provide rhizobacteria with biofertilizing and biopesticide properties. In this study, bacteria from the rhizosphere of tomato were evaluated in vitro for plant growth promotion (PGP) properties. Five Pseudomonas isolates (PsT-04c, PsT-94s, PsT-116, PsT-124, and PsT-130) and one Bacillus isolate (BaT-68s), with the highest ability to solubilize tricalcium phosphate (TCP) were selected for further molecular identification and characterization. Isolates showed phosphate solubilization up to 195.42 µg mL-1. All isolates showed phosphate solubilization by organic acid production. The six isolates improved seed germination and showed effective root colonization when tomato seeds were coated with isolates at 106 cfu g-1 in axenic soil conditions. Furthermore, the selected isolates were tested for beneficial effects on tomato growth and nutrient status in greenhouse experiments with natural rock phosphate (RP). The results showed that inoculated tomato plants in the presence of RP have a higher shoot and root lengths and weights compared with the control. After 60 days, significant increases in plant Ca, Na, P, protein, and sugar contents were also observed in inoculated seedlings. In addition, inoculated tomato seedlings showed an increase in foliar chlorophyll a and b and total chlorophyll, while no significant changes were observed in chlorophyll fluorescence. In greenhouse, two Pseudomonas isolates, PsT-04c and PsT-130, showed ability to trigger induced systemic resistance in inoculated tomato seedlings when subsequently challenged by Clavibacter michiganensis subsp. michiganensis, the causal agent of tomato bacterial canker. High protection rate (75%) was concomitant to an increase in the resistance indicators: total soluble phenolic compounds, phenylalanine-ammonia lyase, and H2O2. The results strongly demonstrated the effectiveness of phosphate-solubilizing bacteria adapted to rhizosphere as biofertilizers for tomato crops and biopesticides by inducing systemic resistance to the causal agent of tomato bacterial canker disease. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
16. Soil and Crop Health Promotion: Zinc and Lead Management by Biofertilization
- Author
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Mousavi, Seyed Majid, Sedaghat, Azadeh, Dheeman, Shrivardhan, editor, Islam, M. Tofazzal, editor, Egamberdieva, Dilfuza, editor, and Siddiqui, Md. Nurealam, editor
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- 2024
- Full Text
- View/download PDF
17. In silico biotechnological potential of Bacillus sp. strain MHSD_37 bacterial endophyte
- Author
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Pfariso Maumela, Adivhaho Khwathisi, Ntakadzeni Edwin Madala, and Mahloro Hope Serepa-Dlamini
- Subjects
Bioremediation ,Biofertilization ,Biocontrol ,Bio-nanotechnology ,Bacterial endophytes ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Endophytic bacteria possess a range of unique characteristics that enable them to successfully interact with their host and survive in adverse environments. This study employed in silico analysis to identify genes, from Bacillus sp. strain MHSD_37, with potential biotechnological applications. Results The strain presented several endophytic lifestyle genes which encode for motility, quorum sensing, stress response, desiccation tolerance and root colonisation. The presence of plant growth promoting genes such as those involved in nitrogen fixation, nitrate assimilation, siderophores synthesis, seed germination and promotion of root nodule symbionts, was detected. Strain MHSD_37 also possessed genes involved in insect virulence and evasion of defence system. The genome analysis also identified the presence of genes involved in heavy metal tolerance, xenobiotic resistance, and the synthesis of siderophores involved in heavy metal tolerance. Furthermore, LC-MS analysis of the excretome identified secondary metabolites with biological activities such as anti-cancer, antimicrobial and applications as surfactants. Conclusions Strain MHSD_37 thereby demonstrated potential biotechnological application in bioremediation, biofertilisation and biocontrol. Moreover, the strain presented genes encoding products with potential novel application in bio-nanotechnology and pharmaceuticals.
- Published
- 2024
- Full Text
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18. In silico biotechnological potential of Bacillus sp. strain MHSD_37 bacterial endophyte
- Author
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Maumela, Pfariso, Khwathisi, Adivhaho, Madala, Ntakadzeni Edwin, and Serepa-Dlamini, Mahloro Hope
- Published
- 2024
- Full Text
- View/download PDF
19. Endophytic Bacillus vallismortis and Bacillus tequilensis bacteria isolated from medicinal plants enhance phosphorus acquisition and fortify Brassica napus L. vegetative growth and metabolic content.
- Author
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Nagah, Aziza, El-Sheekh, Mostafa M., Arief, Omnia M., Alqahtani, Mashael Daghash, Alharbi, Basmah M., and Dawwam, Ghada E.
- Subjects
RAPESEED ,BACILLUS (Bacteria) ,MEDICINAL plants ,PHOSPHORUS ,SOIL productivity - Abstract
Phosphorus fertilization imposes critical limitations on crop productivity and soil health. The aim of the present work is to explore the potential of two phosphate solubilizing bacteria (PSB) species in phosphorus supplementation of canola (Brassica napus L.). Out of 38 bacterial isolates obtained from nine medicinal plants, two bacterial strains (20P and 28P) were proved as the most potent for the in-vitro tricalcium phosphate solubilization test. These isolates verified their activity toward different enzymes as nitrogenase and alkaline phosphatase. Also, 20P and 28P gave a high amount of indole-3-acetic acid, 34.16 mg/ml and 35.20 mg/ml, respectively, and were positive for siderophores production as they detected moderate affinity for iron chelation. Molecular identification confirmed that strain 20P was Bacillus vallismortis and strain 28P was Bacillus tequilensis. A pot experiment was conducted to study the effect of four different phosphorus concentrations (0%, 50%, 75%, and 100% P) each alone and/or in combination with B. vallismortis, B. tequilensis, or both bacterial isolates on the vegetative growth and some physiological parameters of canola. The combined treatment of 50% phosphorus + (B. vallismortis + B. tequilensis) was generally themost effective with respect to shoot height, shoot dry mass, leaf area, photosynthetic pigment fractions, total sugar content, and accumulated NPK content. In contrast, the rhizosphere pH reached the minimum value under the same treatment. These findings highlighted the potential use of PSB (B. vallismortis and B. tequilensis) along with phosphorus fertilization as a safe sustainable tactic. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
20. Co-inoculation of the endophytes Bacillus thuringiensis CAPE95 and Paenibacillus polymyxa CAPE238 promotes Tropaeolum majus L. growth and enhances its root bacterial diversity.
- Author
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Dal'Rio, Isabella, dos Santos Lopes, Eliene, Santaren, Karen Caroline Ferreira, Rosado, Alexandre Soares, and Seldin, Lucy
- Subjects
BACILLUS thuringiensis ,BACTERIAL diversity ,PAENIBACILLUS ,ENDOPHYTIC bacteria ,ENDOPHYTES ,ABIOTIC stress ,NITROGEN fixation ,SPECIES diversity - Abstract
Tropaeolum majus L. is a versatile edible plant that is widely explored due to its medicinal properties and as a key element in intercropping systems. Its growth could be improved by the use of biofertilizers that can enhance nutrient uptake by the plant or provide tolerance to different abiotic and biotic stresses. In a previous study, 101 endophytes isolated from T. majus roots showed more than three plant growth-promoting (PGP) features in vitro, such as phosphate mineralization/solubilization, production of siderophores, antimicrobial substances and indole-related compounds, and presence of the nifH gene. To provide sustainable alternatives for biofertilization, the genomes of two promising endophytes--CAPE95 and CAPE238--were sequenced to uncover metabolic pathways related to biofertilization. Greenhouse experiments were conducted with 216 seeds and 60 seedlings, half co-inoculated with the endophytes (treatment) and half inoculated with 1X PBS (control), and the impact of the co-inoculation on the plant's bacteriome was accessed through 16S rRNA gene metabarcoding. The strains CAPE95 and CAPE238 were taxonomically assigned as Bacillus thuringiensis and Paenibacillus polymyxa, respectively. Metabolic pathways related to the enhancement of nutrient availability (nitrogen fixation, sulfate-sulfur assimilation), biosynthesis of phytohormones (indole-3-acetic acid precursors) and antimicrobial substances (bacilysin, paenibacillin) were found in their genomes. The in vivo experiments showed that treated seeds exhibited faster germination, with a 20.3% higher germination index than the control on the eleventh day of the experiment. Additionally, treated seedlings showed significantly higher plant height and leaf diameters (p < 0.05). The bacterial community of the treated plants was significantly different from that of the control plants (p < 0.001) and showed a higher richness and diversity of species (Chao and Shannon indexes, p < 0.001). A higher relative abundance of potential synergistic PGP bacteria was also shown in the bacteriome of the treated plants, such as Lysinibacillus and Geobacter. For the first time, co-inoculation of B. thuringiensis and P. polymyxa was shown to have great potential for application as a biofertilizer to T. majus plants. The bacterial consortium used here could also be explored in other plant species in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
21. Strategic utilization of microalgae byproducts for sustainable biofertilization of Camellia sinensis (green tea).
- Author
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Çakmak, Ramazan and Uzuner, Ugur
- Subjects
- *
TEA , *GREEN tea , *CHLORELLA vulgaris , *MICROALGAE , *MICROALGAE cultures & culture media , *GREEN algae - Abstract
Green algae are well known for their plant growth-promoting role, but the biofertilization potential of some industrial algae waste has yet to be tested. Industrial algae production facilities are increasing rapidly yearly, but most growing liquids are unused and remain as waste. Here we examined the effects of various microalgae byproducts as tea plant growth biostimulants. The roles of cell-free supernatant (CFS), sonicated microalgae biomass and living cells of the freshwater alga Chlorella vulgaris in promoting tea plant growth were investigated through real field applications. All three algae by-products of the same culture were applied to the tea plants three times either from aerial parts or from the soil. After 40 days of incubation, it was determined that all microalgae waste, even CFS, significantly improved the vegetative growth of the treated tea plants. CFS were also able to improve chlorophyll accumulation, node length, leaf length, and leaf width by 23%-26%, 62%-69%, 5%-36%, and 23%-54%, respectively. Notably, the sonicated microalgae effluents were able to improve the node growth by 123%. To our knowledge, this is the first study revealing the significant biofertilization potential of C. vulgaris CFS for shoot growth of tea plants. The obtained results highlight the agricultural potentials of both microalgae culture broths and degreased microalgae wastes as sustainable and environmentally-friendly biofertilizers for Camellia sinensis organic farming. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
22. Optimizing tomato seedling growth with indigenous mangrove bacterial inoculants and reduced NPK fertilization.
- Author
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Tounsi-Hammami, Soumaya, Khan, Munawwar Ali, Zeb, Aroosa, Anwar, Aneesa Rasheed, Arora, Naman, Naseem, Muhammad, and Mundra, Sunil
- Subjects
MANGROVE plants ,SYNTHETIC fertilizers ,SEEDLINGS ,TOMATOES ,SEEDLING quality ,BACILLUS (Bacteria) - Abstract
The search for ecofriendly products to reduce crop dependence on synthetic chemical fertilizers presents a new challenge. The present study aims to isolate and select efficient native PGPB that can reduce reliance on synthetic NPK fertilizers. A total of 41 bacteria were isolated from the sediment and roots of mangrove trees (Avicennia marina) and assessed for their PGP traits under in vitro conditions. Of them, only two compatible strains of Bacillus species were selected to be used individually and in a mix to promote tomato seedling growth. The efficiency of three inoculants applied to the soil was assessed in a pot experiment at varying rates of synthetic NPK fertilization (0, 50, and 100% NPK). The experiment was set up in a completely randomized design with three replications. Results showed that the different inoculants significantly increased almost all the studied parameters. However, their effectiveness is strongly linked to the applied rate of synthetic fertilization. Applying bacterial inoculant with only 50% NPK significantly increased the plant height (44-51%), digital biomass (60-86%), leaf area (77-87%), greenness average (29-36%), normalized difference vegetation index (29%), shoot dry weight (82-92%) and root dry weight (160-205%) compared to control plants. Concerning the photosynthetic activity, this treatment showed a positive impact on the concentrations of chlorophyll a (25-31%), chlorophyll b (34-39%), and carotenoid (45-49%). Interestingly, these increases ensured the highest values significantly similar to or higher than those of control plants given 100% NPK. Furthermore, the highest accumulation of N, P, K, Cu, Fe, Zn, and Ca in tomato shoots was recorded in plants inoculated with the bacterial mix at 50% NPK. It was proven for the first time that the native PGP bacteria derived from mangrove plant species A. marina positively affects the quality of tomato seedlings while reducing 50% NPK. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
23. Importance of Lactic Acid Bacteria as an Emerging Group of Plant Growth-Promoting Rhizobacteria in Sustainable Agroecosystems.
- Author
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Yaghoubi Khanghahi, Mohammad, Strafella, Sabrina, Filannino, Pasquale, Minervini, Fabio, and Crecchio, Carmine
- Subjects
PLANT growth-promoting rhizobacteria ,LACTIC acid bacteria ,SUSTAINABILITY ,AGRICULTURAL ecology ,SUSTAINABLE agriculture ,AGRICULTURAL chemicals - Abstract
Increasing awareness of the problems caused by synthetic agrochemicals, such as chemical fertilizers, pesticides, and herbicides, makes it crucial to discover substitute approaches that can guarantee competitive plant production and protect the environment while maintaining the natural balance in agroecosystems. One of the leading alternatives is utilizing rhizobacterial strains named plant growth-promoting rhizobacteria (PGPR). The utilization of PGPR-based biofertilizers for advancement in the sustainability of farming productions has received considerable critical attention all over the world because of their contribution to not only improving plant growth but also inducing biotic and abiotic stress tolerance. This review updates the aforementioned eco-friendly strategy in sustainable agroecosystems and provides new insights into the phytostimulation and bioprotection ability of lactic acid bacteria (LAB), an emerging taxon of PGPR. In this regard, the ability of LAB to synthesize metabolites, including organic acids, phenolic acids and their flavonoid derivatives, phytohormones, and antimicrobial substrates, is presented. The use of LAB provides a bridge between PGPR and environmentally friendly crop productivity, which can lead to sustainable production systems by reducing the use of agrochemicals, improving soil quality, and minimizing environmental pollution. All the beneficial aspects of LAB need to be addressed by future research to plan systematic methodologies for their use and/or to combine the use of PGPR along with other organic or inorganic inputs in sustainable production systems. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
24. Effect of biofertilizers and rhizospheric bacteria on growth and root ultrastucture of lettuce.
- Author
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Montesdeoca-Flores, David, Alfayate-Casañas, Carmen, Hernández-Bolaños, Eduardo, Hernández-González, Mercedes, Estupiñan-Afonso, Zuleima, and Abreu-Acosta, Néstor
- Abstract
Biofertilization is a technique that uses plant and animal wastes to add organic matter and nutrients to the soil. It can also use microorganisms that can metabolize these by-products to facilitate their absorption by the plant roots. In this study, we tested the effects of rhizospheric bacteria inoculation (T
1 ), a combination of rhizospheric bacteria with liquid fertilizer (T2 ) and uncombined liquid fertilizer (T3 ), on the growth, nutritional content, root tissue, and root cells of lettuce plants. The results showed significant positive differences in all treatments compared to control plants, in terms of morphological, nutritional, and productivity parameters. The combination of rhizospheric bacteria with liquid SEFEL fertilizer (T2 ) yielded the best results, showing increased fresh and dry weight, and diameter. There were no differences between treatments for nutritional content, but each treatment outperformed the control by more than 700% for all macronutrients. The best result was phosphorus content for T1 , with 1272.22% more than control. Regarding root structure and ultrastructure, there was no variation in tissue organization compared to control plants, but increases in root hairs (T1 ), development of transfer cells (T2 ), and secondary growth (T3 ) were observed. Additionally, colonization of roots by rhizospheric bacteria was confirmed in all three treatments. In conclusion, this study suggests that inoculating with rhizospheric bacteria is a viable and environmentally friendly biofertilization for lettuce plants. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
25. Phosphate solubilizing Pseudomonas and Bacillus combined with rock phosphates promoting tomato growth and reducing bacterial canker disease
- Author
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Mohamed Bakki, Badra Banane, Omaima Marhane, Qassim Esmaeel, Abdelhakim Hatimi, Essaid Ait Barka, Khalid Azim, and Brahim Bouizgarne
- Subjects
Bacillus ,biofertilization ,rock phosphate ,plant growth promotion ,Pseudomonas ,solubilization ,Microbiology ,QR1-502 - Abstract
Nowadays, sustainable agriculture approaches are based on the use of biofertilizers and biopesticides. Tomato (Solanum lycopersicum L.) rhizosphere could provide rhizobacteria with biofertilizing and biopesticide properties. In this study, bacteria from the rhizosphere of tomato were evaluated in vitro for plant growth promotion (PGP) properties. Five Pseudomonas isolates (PsT-04c, PsT-94s, PsT-116, PsT-124, and PsT-130) and one Bacillus isolate (BaT-68s), with the highest ability to solubilize tricalcium phosphate (TCP) were selected for further molecular identification and characterization. Isolates showed phosphate solubilization up to 195.42 μg mL−1. All isolates showed phosphate solubilization by organic acid production. The six isolates improved seed germination and showed effective root colonization when tomato seeds were coated with isolates at 106 cfu g−1 in axenic soil conditions. Furthermore, the selected isolates were tested for beneficial effects on tomato growth and nutrient status in greenhouse experiments with natural rock phosphate (RP). The results showed that inoculated tomato plants in the presence of RP have a higher shoot and root lengths and weights compared with the control. After 60 days, significant increases in plant Ca, Na, P, protein, and sugar contents were also observed in inoculated seedlings. In addition, inoculated tomato seedlings showed an increase in foliar chlorophyll a and b and total chlorophyll, while no significant changes were observed in chlorophyll fluorescence. In greenhouse, two Pseudomonas isolates, PsT-04c and PsT-130, showed ability to trigger induced systemic resistance in inoculated tomato seedlings when subsequently challenged by Clavibacter michiganensis subsp. michiganensis, the causal agent of tomato bacterial canker. High protection rate (75%) was concomitant to an increase in the resistance indicators: total soluble phenolic compounds, phenylalanine-ammonia lyase, and H2O2. The results strongly demonstrated the effectiveness of phosphate-solubilizing bacteria adapted to rhizosphere as biofertilizers for tomato crops and biopesticides by inducing systemic resistance to the causal agent of tomato bacterial canker disease.
- Published
- 2024
- Full Text
- View/download PDF
26. Technical and Economic Assessment of Tomato Cultivation Through a Macro-Tunnel Production System with the Application of Gluconacetobacter diazotrophicus
- Author
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Nelson Ceballos-Aguirre, Alejandro Hurtado-Salazar, Gloria M. Restrepo, Óscar J. Sánchez, María C. Hernández, and Mauricio Montoya
- Subjects
biofertilization ,economic indicators ,microbial inoculants ,nitrogen fixation ,plant growth promoting bacteria ,Plant culture ,SB1-1110 - Abstract
Bacterial inoculants hold promise for enhancing the sustainability and profitability of tomato cultivation in macro-tunnel systems. This study aimed to evaluate the technical and economic viability of applying Gluconacetobacter diazotrophicus to tomato production. The separate addition of native G. diazotrophicus GIBI025 and GIBI029 isolates and a commercial inoculant containing Azotobacter chrococcum and Azospirillium sp. was evaluated at a rate of 1 × 108 CFU·mL−1 without nitrogen addition. Conventional fertilization treatment with no bacteria added and 100%-nitrogen fertilization relative to crop requirements (added as MAP and urea) was also assessed. The treatments were evaluated within the macro-tunnel production system. The experiment utilized a completely randomized block design with four replications per treatment, and each experimental unit consisted of 20 plants. The yield (kg·ha−1) was calculated and economic assessment was performed. The results show that native G. diazotrophicus isolates in tomato cultivation under the macro-tunnel production system improved its economic viability, achieving yields up to 95,501 kg·ha−1 without the addition of nitrogenous fertilizers. This research reveals benefit–cost ratios achieving 1.57 and net incomes reaching 16,707 US dollars per hectare. This work demonstrated that the native isolates assessed may be used in the pursuit of more integrated, sustainable, and competitive cultural practices.
- Published
- 2024
- Full Text
- View/download PDF
27. Endophytic Bacillus vallismortis and Bacillus tequilensis bacteria isolated from medicinal plants enhance phosphorus acquisition and fortify Brassica napus L. vegetative growth and metabolic content
- Author
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Aziza Nagah, Mostafa M. El-Sheekh, Omnia M. Arief, Mashael Daghash Alqahtani, Basmah M. Alharbi, and Ghada E. Dawwam
- Subjects
biofertilization ,canola ,phosphate solubilization ,Bacillus vallismortis ,Bacillus tequilensis ,metabolic content ,Plant culture ,SB1-1110 - Abstract
Phosphorus fertilization imposes critical limitations on crop productivity and soil health. The aim of the present work is to explore the potential of two phosphate solubilizing bacteria (PSB) species in phosphorus supplementation of canola (Brassica napus L.). Out of 38 bacterial isolates obtained from nine medicinal plants, two bacterial strains (20P and 28P) were proved as the most potent for the in-vitro tricalcium phosphate solubilization test. These isolates verified their activity toward different enzymes as nitrogenase and alkaline phosphatase. Also, 20P and 28P gave a high amount of indole-3-acetic acid, 34.16 μg/ml and 35.20 μg/ml, respectively, and were positive for siderophores production as they detected moderate affinity for iron chelation. Molecular identification confirmed that strain 20P was Bacillus vallismortis and strain 28P was Bacillus tequilensis. A pot experiment was conducted to study the effect of four different phosphorus concentrations (0%, 50%, 75%, and 100% P) each alone and/or in combination with B. vallismortis, B. tequilensis, or both bacterial isolates on the vegetative growth and some physiological parameters of canola. The combined treatment of 50% phosphorus + (B. vallismortis + B. tequilensis) was generally the most effective with respect to shoot height, shoot dry mass, leaf area, photosynthetic pigment fractions, total sugar content, and accumulated NPK content. In contrast, the rhizosphere pH reached the minimum value under the same treatment. These findings highlighted the potential use of PSB (B. vallismortis and B. tequilensis) along with phosphorus fertilization as a safe sustainable tactic.
- Published
- 2024
- Full Text
- View/download PDF
28. Optimizing tomato seedling growth with indigenous mangrove bacterial inoculants and reduced NPK fertilization
- Author
-
Soumaya Tounsi-Hammami, Munawwar Ali Khan, Aroosa Zeb, Aneesa Rasheed Anwar, Naman Arora, Muhammad Naseem, and Sunil Mundra
- Subjects
Bacillus sp. ,sustainable agriculture ,biofertilization ,nutrient management ,Avicennia marina ,Plant culture ,SB1-1110 - Abstract
The search for ecofriendly products to reduce crop dependence on synthetic chemical fertilizers presents a new challenge. The present study aims to isolate and select efficient native PGPB that can reduce reliance on synthetic NPK fertilizers. A total of 41 bacteria were isolated from the sediment and roots of mangrove trees (Avicennia marina) and assessed for their PGP traits under in vitro conditions. Of them, only two compatible strains of Bacillus species were selected to be used individually and in a mix to promote tomato seedling growth. The efficiency of three inoculants applied to the soil was assessed in a pot experiment at varying rates of synthetic NPK fertilization (0, 50, and 100% NPK). The experiment was set up in a completely randomized design with three replications. Results showed that the different inoculants significantly increased almost all the studied parameters. However, their effectiveness is strongly linked to the applied rate of synthetic fertilization. Applying bacterial inoculant with only 50% NPK significantly increased the plant height (44-51%), digital biomass (60-86%), leaf area (77-87%), greenness average (29-36%), normalized difference vegetation index (29%), shoot dry weight (82-92%) and root dry weight (160-205%) compared to control plants. Concerning the photosynthetic activity, this treatment showed a positive impact on the concentrations of chlorophyll a (25-31%), chlorophyll b (34-39%), and carotenoid (45-49%). Interestingly, these increases ensured the highest values significantly similar to or higher than those of control plants given 100% NPK. Furthermore, the highest accumulation of N, P, K, Cu, Fe, Zn, and Ca in tomato shoots was recorded in plants inoculated with the bacterial mix at 50% NPK. It was proven for the first time that the native PGP bacteria derived from mangrove plant species A. marina positively affects the quality of tomato seedlings while reducing 50% NPK.
- Published
- 2024
- Full Text
- View/download PDF
29. Co-inoculation of the endophytes Bacillus thuringiensis CAPE95 and Paenibacillus polymyxa CAPE238 promotes Tropaeolum majus L. growth and enhances its root bacterial diversity
- Author
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Isabella Dal’Rio, Eliene dos Santos Lopes, Karen Caroline Ferreira Santaren, Alexandre Soares Rosado, and Lucy Seldin
- Subjects
Tropaeolum majus ,Bacillus thuringiensis ,Paenibacillus polymyxa ,biofertilization ,plant growth-promoting bacteria ,Microbiology ,QR1-502 - Abstract
Tropaeolum majus L. is a versatile edible plant that is widely explored due to its medicinal properties and as a key element in intercropping systems. Its growth could be improved by the use of biofertilizers that can enhance nutrient uptake by the plant or provide tolerance to different abiotic and biotic stresses. In a previous study, 101 endophytes isolated from T. majus roots showed more than three plant growth-promoting (PGP) features in vitro, such as phosphate mineralization/solubilization, production of siderophores, antimicrobial substances and indole-related compounds, and presence of the nifH gene. To provide sustainable alternatives for biofertilization, the genomes of two promising endophytes—CAPE95 and CAPE238—were sequenced to uncover metabolic pathways related to biofertilization. Greenhouse experiments were conducted with 216 seeds and 60 seedlings, half co-inoculated with the endophytes (treatment) and half inoculated with 1X PBS (control), and the impact of the co-inoculation on the plant’s bacteriome was accessed through 16S rRNA gene metabarcoding. The strains CAPE95 and CAPE238 were taxonomically assigned as Bacillus thuringiensis and Paenibacillus polymyxa, respectively. Metabolic pathways related to the enhancement of nutrient availability (nitrogen fixation, sulfate-sulfur assimilation), biosynthesis of phytohormones (indole-3-acetic acid precursors) and antimicrobial substances (bacilysin, paenibacillin) were found in their genomes. The in vivo experiments showed that treated seeds exhibited faster germination, with a 20.3% higher germination index than the control on the eleventh day of the experiment. Additionally, treated seedlings showed significantly higher plant height and leaf diameters (p
- Published
- 2024
- Full Text
- View/download PDF
30. Biofertilización orgánica y mineral en posturas de Tithonia diversifolia (Hemsl.) A. Gray en fase de vivero.
- Author
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Lezcano Más, Yohanka, Milián Florido, Grethel, and Placeres Espinosa, Iraní
- Subjects
- *
TITHONIA diversifolia , *SECONDARY metabolism , *METABOLISM , *BIOCHAR , *PLANT nurseries - Abstract
Background: The current challenge is to produce food in systems that are resilient to climate change and take care of the environment. Aim. Evaluate organic and mineral biofertilization in the growth and development of Tithonia diversifolia positions during the nursery phase. Materials and methods: Two trials were carried out: E-I at the “Renato Guitar” CPA and E-II: at the Indio Hatuey Pasture and Forage Experimental Station (EEPFIH). For their development, a completely randomized experimental design was used with 15 and 10 replications, experiment I and II respectively. E-I, three treatments were carried out: T-1: 100% unfertilized soil, T-2: 50% soil + 50% biochar, T-3: 50% soil + 25% biochar + 25% cane bagasse ash. The biochar was obtained from marabou (D. cinerea) and was always embedded in efficient IHPLUS®BF microorganisms. Physiological parameters of primary and secondary metabolism were measured at 30 days, and morphological growth indicators at 30 and 60 days. In E-II, 5 treatments were used: T-1: Unfertilized soil, T-2: Combination of 50% soil-50% compost + Rhizobium, T-3: Combination of 50% soil-50% compost + Trichoderma, T-4: Combination of 50% soil-50% compost with micronized dolomite + Rhizobium and T-5: Combination of 50% soil-50% compost with micronized dolomite + Trichoderma. Morphological growth indicators were measured at 60 days and production of dry aerial biomass at the time of transplant. Results: the results show that in Trial-I the best variant was T-3: soil 50%+IHPLUS®BF enriched Biochar 25%+ash 25%. Test-II, all the variants studied were superior to the control. Conclusion: The results confirmed the effectiveness of these alternatives to grow T. diversifolia plants under nursery conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
31. Endophytic Microbes and Their Role in Plant Health
- Author
-
Krishnappa, Charishma, Kavya, B. S., Akshay Kumar, H. M., Reddy, Priya, Rajeshwar Rao, G., Darshan, K., Sharma, Anil Kumar, Series Editor, Mathur, Piyush, editor, Kapoor, Rupam, editor, and Roy, Swarnendu, editor
- Published
- 2023
- Full Text
- View/download PDF
32. Advanced Biotechnological Tools for Improving Phosphorus Use Efficiency
- Author
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Malik, Hafiza Aasia, Rahman, Atta Ur, Akbar, Fazal, Ahmad, Nisar, Ali, Syed Shujait, Suleman, Muhammad, Ali, Shahid, Hussain, Zahid, Zaman, Nasib, Rasool, Akhtar, Shah, Muzafar, Israr, Muhammad, Iqbal, Asif, Lichtfouse, Eric, Series Editor, Ranjan, Shivendu, Advisory Editor, Dasgupta, Nandita, Advisory Editor, Iqbal, Asif, editor, Iqbal, Mazhar, editor, Alamzeb, Madeeha, editor, Meizhen, Song, editor, Xiling, Zhang, editor, Arif, Muhammad, editor, and Du, Xiongming, editor
- Published
- 2023
- Full Text
- View/download PDF
33. Applications of the Microalgae Chlamydomonas and Its Bacterial Consortia in Detoxification and Bioproduction
- Author
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María J. Torres, Carmen M. Bellido-Pedraza, and Angel Llamas
- Subjects
algal–microbial consortia ,microalga ,Chlamydomonas ,bioremediation ,biofertilization ,high-value added products ,Science - Abstract
The wide metabolic diversity of microalgae, their fast growth rates, and low-cost production make these organisms highly promising resources for a variety of biotechnological applications, addressing critical needs in industry, agriculture, and medicine. The use of microalgae in consortia with bacteria is proving valuable in several areas of biotechnology, including the treatment of various types of wastewater, the production of biofertilizers, and the extraction of various products from their biomass. The monoculture of the microalga Chlamydomonas has been a prominent research model for many years and has been extensively used in the study of photosynthesis, sulphur and phosphorus metabolism, nitrogen metabolism, respiration, and flagellar synthesis, among others. Recent research has increasingly recognised the potential of Chlamydomonas–bacteria consortia as a biotechnological tool for various applications. The detoxification of wastewater using Chlamydomonas and its bacterial consortia offers significant potential for sustainable reduction of contaminants, while facilitating resource recovery and the valorisation of microalgal biomass. The use of Chlamydomonas and its bacterial consortia as biofertilizers can offer several benefits, such as increasing crop yields, protecting crops, maintaining soil fertility and stability, contributing to CO2 mitigation, and contributing to sustainable agricultural practises. Chlamydomonas–bacterial consortia play an important role in the production of high-value products, particularly in the production of biofuels and the enhancement of H2 production. This review aims to provide a comprehensive understanding of the potential of Chlamydomonas monoculture and its bacterial consortia to identify current applications and to propose new research and development directions to maximise their potential.
- Published
- 2024
- Full Text
- View/download PDF
34. Plant-Growth-Promoting Rhizobacteria Improve Seeds Germination and Growth of Argania spinosa
- Author
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Naima Chabbi, Salahddine Chafiki, Maryem Telmoudi, Said Labbassi, Rachid Bouharroud, Abdelghani Tahiri, Rachid Mentag, Majda El Amri, Khadija Bendiab, Driss Hsissou, Abdelaziz Mimouni, Naima Ait Aabd, and Redouan Qessaoui
- Subjects
Argania spinosa ,PGPR ,seedling ,plant growth ,biofertilization ,Botany ,QK1-989 - Abstract
Argania spinosa is among the most important species of the Moroccan forest in terms of ecological, environmental, and socio-economic aspects. However, it faces a delicate balance between regeneration and degradation in its natural habitat. Hence, the efforts to preserve and regenerate argan forests are crucial for biodiversity, soil quality, and local livelihoods, yet they face challenges like overgrazing and climate change. Sustainable management practices, including reforestation and community engagement, are vital for mitigating degradation. Similarly, exploiting the argan tree’s rhizosphere can enhance soil quality by leveraging its rich microbial diversity. This approach not only improves crop growth but also maintains ecosystem balance, ultimately benefiting both agriculture and the environment. This enrichment can be achieved by different factors: mycorrhizae, plant extracts, algae extracts, and plant growth-promoting rhizobacteria (PGPR). The benefits provided by PGPR may include increased nutrient availability, phytohormone production, shoot, root development, protection against several plant pathogens, and disease reduction. In this study, the effect of rhizobacteria isolated from the Agran rhizosphere was evaluated on germination percentage and radicle length for Argania spinosa in vitro tests, growth, collar diameter, and branching number under greenhouse conditions. One hundred and twenty (120) bacteria were isolated from the argan rhizosphere and evaluated for their capacity for phosphate solubilization and indole acetic acid production. The results showed that 52 isolates could solubilize phosphorus, with the diameters of the solubilization halos varying from 0.56 ± 0.14 to 2.9 ± 0.08 cm. Among 52 isolates, 25 were found to be positive for indole acetic acid production. These 25 isolates were first tested on maize growth to select the most performant ones. The results showed that 14 isolates from 25 tested stimulated maize growth significantly, and 3 of them by 28% (CN005, CN006, and CN009) compared to the control. Eight isolates (CN005, CN006, CN004, CN007, CN008, CN009, CN010, and CN011) that showed plant growth of more than 19% were selected to evaluate their effect on argan germination rate and radicle length and were subjected to DNA extraction and conventional Sanger sequencing. The 8 selected isolates were identified as: Brevundimonas naejangsanensis sp2, Alcaligenes faecalis, Brevundimonas naejangsanensis sp3, Brevundimonas naejangsanensis sp4, Leucobacter aridicollis sp1, Leucobacter aridicollis sp2, Brevundimonas naejangsanensis sp1, and Staphylococcus saprophyticus. The results showed that Leucobacter aridicollis sp2 significantly increased the germination rate by 95.83%, and the radicle length with a value of 2.71 cm compared to the control (1.60 cm), followed by Brevundimonas naejangsanensis sp3 and Leucobacter aridicollis sp1 (2.42 cm and 2.11 cm, respectively). Under greenhouse conditions, the results showed that the height growth increased significantly for Leucobacter aridicollis sp1 (42.07%) and Leucobacter aridicollis sp2 (39.99%). The isolates Brevundimonas naejangsanensis sp3 and Leucobacter aridicollis sp1 increased the gain of collar diameter by 41.56 and 41.21%, respectively, followed by Leucobacter aridicollis sp2 and Staphyloccocus saprophyticus (38.68 and 22.79%). Leucobacter aridicollis sp1 increased the ramification number per plant to 12 compared to the control, which had 6 ramifications per plant. The use of these isolates represents a viable alternative in sustainable agriculture by improving the germination rate and root development of the argan tree, as well as its development, while increasing the availability of nutrients in the soil and consequently improving fertilization.
- Published
- 2024
- Full Text
- View/download PDF
35. Improving soil fertility through dual inoculation with arbuscular mycorrhizal fungi and Rhizobium on a eutric cambisol cultivated with forage legumes in a semi-arid region
- Author
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Sanele Mpongwana, Alen Manyevere, Johnfisher Mupangwa, Conference Thando Mpendulo, and Chuene Victor Mashamaite
- Subjects
Arbuscular mycorrhizal fungi ,Biofertilization ,Legumes ,Chemical properties ,Rhizobium ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
The Sub-Saharan region of southern Africa is characterized by high temperatures, low rainfall, and poor land-use management practices such as continuous cropping without replenishment of soil nutrients. The combination of these factors has resulted in nutrient depletion and land degradation. The current study aimed at investigating the effect of arbuscular mycorrhizal fungi (AMF) and Rhizobium bacteria inoculation on soil chemical properties in field-grown forage legumes, namely, Mucuna pruriens (mucuna), Lablab purpureus (lablab) and Vigna unguiculata (cowpea), in the semi-arid region of the Eastern Cape Province (South Africa). Forage legumes were inoculated with the AMF species Paraglomus occulum and the Rhizobia bacteria species Bradyrhizobium strain and grown for 120 days. Soil samples were collected in the following sequence: prior to planting, before flowering and after harvesting the forage legumes in each of the two seasons (2017/2018 and 2018/2019) and soil chemical properties were determined using standard procedures. The results showed that the addition of dual inoculation over time greatly improved soil chemical properties when compared to the control treatment. This was advocated by the significant (P ≤ 0.05) increase in soil pH, soil organic carbon, soil organic matter, total nitrogen, phosphorus, calcium, potassium, magnesium, sodium, sulfur and iron in soils. The concentration of cation exchange capacity was significantly (P ≤ 0.05) higher in cowpea treated with Rhizobium as compared to other treatment combinations. The control treatment of mucuna forage greatly improved the concentrations of manganese, boron, copper, molybdenum, and zinc over other treatment combinations only before the flowering stage. However, the concentrations of micronutrients were significantly higher on the treatment combination of lablab and single inoculation of AMF after harvesting. Generally, dual inoculation with AMF and Rhizobia enhanced soil properties when compared to a single inoculation or untreated control.
- Published
- 2024
- Full Text
- View/download PDF
36. Potential of Bacillus toyonensis and Paenibacillus alvei as Plant Growth Promoter on Melon Manis Terengganu (MMT)
- Author
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Mariana Mohammad, Noor A. Badaluddin, Mohammad M. Khandaker, and Eeyad A. Asri
- Subjects
Biofertilization ,Cucumis melo ,Effective microorganisms ,Agriculture (General) ,S1-972 - Abstract
Cucumis melo var inodorus cv. Manis Terengganu is known as Terengganu’s iconic fruits. However, MMT production is still insufficient to satisfy local demand. Over-reliance on chemical fertilizers to MMT has reduced soil fertility, degraded soil quality resistance, and negatively affected the environment. As a result, efficient microorganisms such as Paenibacillus alvei and Bacillus toyonensis were investigated as possible ecologically compatible biofertilisers for MMT. In this study, B. toyonensis and P. alvei produced indole-3-acetic acid (IAA) at concentrations of 20.49 µg.mL-1 and 19.18 µg.mL-1, respectively. hen, the greenhouse testing was conducted to evaluate the potentiality of effective microbes as plant growth promoter rhizobacteria by introducing the soil with B. toyonensis, P. alvei, and consortium. The experiment was laid out in a randomized complete block design (RCBD) with three groups of bacteria plus the control and each treatment replicated 15 times. Data were analyzed according to ANOVA procedure. As a result, B. toyonensis, P. alvei, and consortium significantly increased in all experimental growth parameters such as plant height, leaves, chlorophyll content, root mass, root length, leaf area, fruit weight, macronutrients, and micronutrients compared to control. Overall, the results showed that the effective single microbial strains promoted the development of MMT more effectively than the consortium
- Published
- 2023
37. Phosphate solubilization and the enhancement of chickpea growth by new rhizospheric microorganisms Bacillus tequilensis and Trichoderma orientale.
- Author
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Bekkar, Ahmed Amine and Zaim, Souad
- Subjects
- *
BACILLUS (Bacteria) , *TRICHODERMA , *SOLUBILIZATION , *MICROORGANISMS , *SEED development - Abstract
Two Trichoderma strains and three Bacillus strains isolated from the rhizosphere of healthy chickpeas in Algeria were assessed for their phosphate solubilizing capacity in vitro as well as their growth effects on seedlings of the chickpea in pot experiments. The microorganisms tested had higher phosphate-solubilizing activities, with the solubilization index ranging from 2.41 to 7.40. The concentration of solubilized phosphate varied from 30.17 to 157.44 µg/mL. The maximum phosphate-solubilizing activity was observed in the two culture filtrates of Bacillus tequilensis Bt1 (157.44 µg/mL) and Trichoderma orientale T1 (143.33 µg/mL), accompanied by a decrease in pH of the growth medium from 4.51 to 5.75. The application of the strains (B. tequilensis Bt1 and T. orientale T1) separately and in combination had a beneficial effect on germination by promoting the development of the seeds and effectively enhancing plant growth. Chickpea seedlings showed better vegetative growth when treated with a mixture of B. tequilensis Bt1 and T. orientale T1 together than an individual treatment. To our knowledge, this is the first report of the phosphate-solubilizing potential of the combined microorganisms B. tequilensis and T. orientale and their capacity to promote plant growth in chickpeas. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
38. The effect of biofertilization and organic fertilization and the interaction between them on the yield characteristics and components of the mung bean plant Vigna radiata L.
- Author
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Juma Al-Zubaidi, Najm Abdullah and Alwan Al-Shammari, Abeer Fadel
- Subjects
MUNG bean ,AUTUMN ,HUMIC acid ,CLAY soils ,AZOTOBACTER - Abstract
A field experiment was conducted in the fall season of 2022 in the village of Aday in Baquba District - Diyala Governorate, on the mash plant. Vigna radaita a local vegetable cultivar in clay soil in order to study the effect of biofertilization with Azotobacter chroococcum and Mycorhiza fungi (Glomus mosseae) and organic fertilization with humic acid and the interaction between them on the vegetative characteristics of the mung bean plant. The experiment was carried out according to the randomized complete block design (R.C.B.D) and with three replications. It included two factors, the first was biological fertilization, which included four treatments, namely (without addition, the addition of Azotobacter, the addition of Mycorrhiza, and the addition of Azotobacter + Mycorrhiza) and the second factor was organic fertilization, which included four concentrations of humic acid 0 and 3 And 6 and 9 ml.l
-1 . The following are the most important results that were reached: - The superiority of biofertilization treatment by adding Azotobacter and Mycorrhizae in the yield and its components, such as the number of pods, the weight of 1000 seeds, the yield of one plant, the overall yield, obtained, Azolobacter and mycorrhizae were introduced to the biofertilization process., 130.83 pods.plant-1 , 29.26 gm., 35.57 g.m. plant-1 , 2341.08 kg.h-1 . - The treatment of organic fertilization with humic acid at a concentration of 9 ml.L-1 was superior in giving it the highest mean in the number of pods, the weight of 1000 seeds, yield per plant, overall yield, which reached 127.14 pod.plant-1 , 27.76 gm., 36.2 gm. plant-1 , 2221.2 kg.h-1 . [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
39. Exopolysaccharide production in Ensifer meliloti laboratory and native strains and their effects on alfalfa inoculation
- Author
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Primo, Emiliano D, Cossovich, Sacha, Nievas, Fiorela, Bogino, Pablo, Humm, Ethan A, Hirsch, Ann M, and Giordano, Walter
- Subjects
Microbiology ,Biological Sciences ,Zero Hunger ,Bacterial Proteins ,Fertilizers ,Galactans ,Gene Expression Regulation ,Bacterial ,Glucans ,Medicago sativa ,Plant Root Nodulation ,Polysaccharides ,Bacterial ,Sinorhizobium meliloti ,Symbiosis ,Alfalfa ,Ensifer meliloti ,Exopolysaccharides ,Biofertilization ,Medical Microbiology - Abstract
Bacterial surface molecules have an important role in the rhizobia-legume symbiosis. Ensifer meliloti (previously, Sinorhizobium meliloti), a symbiotic Gram-negative rhizobacterium, produces two different exopolysaccharides (EPSs), termed EPS I (succinoglycan) and EPS II (galactoglucan), with different functions in the symbiotic process. Accordingly, we undertook a study comparing the potential differences in alfalfa nodulation by E. meliloti strains with differences in their EPSs production. Strains recommended for inoculation as well as laboratory strains and native strains isolated from alfalfa fields were investigated. This study concentrated on EPS-II production, which results in mucoid colonies that are dependent on the presence of an intact expR gene. The results revealed that although the studied strains exhibited different phenotypes, the differences did not affect alfalfa nodulation itself. However, subtle changes in timing and efficacy to the effects of inoculation with the different strains may result because of other as-yet unknown factors. Thus, additional research is needed to determine the most effective inoculant strains and the best conditions for improving alfalfa production under agricultural conditions.
- Published
- 2020
40. Selección de rizobios eficientes en líneas de frijol común (Phaseolus vulgaris L.) tolerantes a sequía.
- Author
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CUBILLOS-HINOJOSA, JUAN-GUILLERMO, SUÁREZ-FRAGOZO, ELVA-CECILIA, AGUIRRE-PÉREZ, LAURA, GÓMEZ-RAMÍREZ, LUIS-FERNANDO, and TOFIÑO-RIVERA, ADRIANA-PATRICIA
- Subjects
- *
NITROGEN fixation , *COMMON bean , *PLANT adaptation , *ABIOTIC stress , *AGRICULTURAL productivity , *LEGUMES , *PLANT growth , *PLANT nutrients , *RHIZOBACTERIA - Abstract
Common bean (Phaseolus vulgaris L.) yield is limited by prolonged drought conditions and nutrients scarcity in the soil. The Colombian Agricultural Research Corporation (AGROSAVIA) selected five common bean genotypes for presenting biofortification characteristics, good grain size and water deficit tolerance, however, sustainable techniques are required that contribute to the adaptation of these genotypes, and mitigate the impact of drought and nutrient scarcity on the crop production. Plant growth promoting rhizobacteria is an alternative to improve nutrients absorption and plants adaptation to adverse conditions. In this work, the efficiency of rhizobia in biological nitrogen fixation (BNF) in selected common bean genotypes was evaluated. Soil samples were taken in the rhizosphere and roots of the five bean genotypes at the Motilonia Research Center, as well as two alternative samplings in other areas of the Cesar department. Bean seeds were used as a trap to obtain nodules in the laboratory. The nodulation and biological nitrogen fixation of 11 strains from the AGROSAVIA bank in five bean genotypes were evaluated and the symbiotic efficiency of 7 of the strains in four of the genotypes was measured. Three isolates with rhizobia characteristics, symbionts of the 77-SMG22 genotype, were obtained. The most efficient strains in BNF were P17 and P22 in genotype 45-HTA10-2, strains C229 and P37 in 48-HTA14-1, in genotype 56-DAB295 all symbiont strains were efficient (C229, P03, P22 and P37), and in the 77-SMG22 genotype the most efficient treatment was the commercial inoculant Rhizobiol®. Therefore, the strains from the AGROSAVIA bank promote the development of some common bean genotypes reflected in the growth of the plants. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
41. The effect of biofertilization and organic fertilization and the interaction between them on the yield characteristics and components of the mung bean plant Vigna radiata L.
- Author
-
Al-Zubaidi, Najm Abdullah Juma and Al-Shammari, Abeer Fadel Alwan
- Subjects
MUNG bean ,AUTUMN ,HUMIC acid ,CLAY soils ,AZOTOBACTER - Abstract
A field experiment was conducted in the fall season of 2022 in the village of Aday in Baquba District - Diyala Governorate, on the mash plant. Vigna radaita a local vegetable cultivar in clay soil in order to study the effect of biofertilization with Azotobacter chroococcum and Mycorhiza fungi (Glomus mosseae) and organic fertilization with humic acid and the interaction between them on the vegetative characteristics of the mung bean plant. The experiment was carried out according to the randomized complete block design (R.C.B.D) and with three replications. It included two factors, the first was biological fertilization, which included four treatments, namely (without addition, the addition of Azotobacter, the addition of Mycorrhiza, and the addition of Azotobacter + Mycorrhiza) and the second factor was organic fertilization, which included four concentrations of humic acid 0 and 3 And 6 and 9 ml.l
-1 . The following are the most important results that were reached: - The superiority of biofertilization treatment by adding Azotobacter and Mycorrhizae in the yield and its components, such as the number of pods, the weight of 1000 seeds, the yield of one plant, the overall yield, obtained, Azolobacter and mycorrhizae were introduced to the biofertilization process., 130.83 pods.plant-1 , 29.26 gm., 35.57 g.m. plant-1 , 2341.08 kg.h-1 . - The treatment of organic fertilization with humic acid at a concentration of 9 ml.L-1 was superior in giving it the highest mean in the number of pods, the weight of 1000 seeds, yield per plant, overall yield, which reached 127.14 pod.plant-1 , 27.76 gm., 36.2 gm. plant-1 , 2221.2 kg.h-1 . [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
42. Investigating the effects of plant growth-promoting rhizobacteria isolates on germination and physiology status of durum wheat under salt stress.
- Author
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BOUFARES, Khaled, KOUADRIA, Mostefa, KARIMA, Mohamedi, and MERDJET, Yahia Naima
- Subjects
DURUM wheat ,PLANT growth ,RHIZOBACTERIA - Abstract
Copyright of Acta Agriculturae Slovenica is the property of Biotechnical Faculty of the University of Ljubljana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
43. Mealworm Frass as a Potential Organic Fertilizer in Synergy with PGP-Based Biostimulant for Lettuce Plants.
- Author
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Fuertes-Mendizábal, Teresa, Salcedo, Isabel, Huérfano, Ximena, Riga, Patrick, Estavillo, José María, Ávila Blanco, David, and Duñabeitia, Miren Karmele
- Subjects
- *
ORGANIC fertilizers , *GREENHOUSES , *ORGANIC farming , *ROOT development , *NUTRIENT uptake , *MICROBIAL diversity , *LETTUCE - Abstract
This study explores the potential use of frass, the larval excrement residue obtained from mealworm rearing, as organic fertilizer for crops. Its high organic matter content means that its joint application with a biostimulant based on efficient microorganisms, favoring its mineralization, is of interest. An experiment with lettuce plants (Lactuca sativa L.) was conducted with two factors and six replicates under greenhouse conditions. The first factor was frass amendment at 0%, 1%, 2.5%, and 5% of the peat substrate, and the second factor was a Bacillus-based BS at two levels, with and without efficient microorganism application. The results reveal that frass shows great potential as an organic fertilizer, providing macronutrients and increasing lettuce aerial biomass, although its effect is mediated by the application rate. Rates of 2.5% or higher proved negative for lettuce plant growth, especially root development, probably due to an increased incidence of potentially pathogenic fungi. The negative effect of medium–high frass rates was counteracted by the addition of a PGP-based biostimulant, enhancing lettuce plant nutrient uptake, aerial biomass, and quality in terms of succulence, but also favoring microbial diversity in the rhizosphere, increasing the incidence of beneficial microorganisms, and decreasing potentially pathogenic fungi. This positive synergy observed between frass and the PGP-based biostimulant is of interest for the design of new organic fertilization strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
44. Responses in Nodulated Bean (Phaseolus vulgaris L.) Plants Grown at Elevated Atmospheric CO 2.
- Author
-
Bellido, Enrique, de la Haba, Purificación, and Agüera, Eloísa
- Subjects
ATMOSPHERIC carbon dioxide ,COMMON bean ,LEGUMES ,BEANS ,PLANT fertilization ,RHIZOBIUM leguminosarum ,AGRICULTURE ,CARBON metabolism - Abstract
The increase in the concentration of CO
2 in the atmosphere is currently causing metabolomic and physiological changes in living beings and especially in plants. Future climate change may affect crop productivity by limiting the uptake of soil resources such as nitrogen (N) and water. The contribution of legume–rhizobia symbioses to N2 fixation increases the available biological N reserve. Elevated CO2 (eCO2 ) has been shown to enhance the amount of fixed N2 primarily by increasing biomass. Greater leaf biomass under eCO2 levels increases N demand, which can stimulate and increase N2 fixation. For this reason, bean plants (Phaseolus vulgaris L.) were used in this work to investigate how, in a CO2 -enriched atmosphere, inoculation with rhizobia (Rhizobium leguminosarum) affects different growth parameters and metabolites of carbon and nitrogen metabolism, as well as enzymatic activities of nitrogen metabolism and the oxidative state of the plant, with a view to future scenarios, where the concentration of CO2 in the atmosphere will increase. The results showed that bean symbiosis with R. leguminosarum improved N2 fixation, while also decreasing the plant's oxidative stress, and provided the plant with a greater defense system against eCO2 conditions. In conclusion, the nodulation with rhizobia potentially replaced the chemical fertilization of bean plants (P. vulgaris L.), resulting in more environmentally friendly agricultural practices. However, further optimization of symbiotic activities is needed to improve the efficiency and to also develop strategies to improve the response of legume yields to eCO2, particularly due to the climate change scenario in which there is predicted to be a large increase in the atmospheric CO2 concentration. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
45. Understanding the sugar beet holobiont for sustainable agriculture.
- Author
-
Wolfgang, Adrian, Temme, Nora, Tilcher, Ralf, and Berg, Gabriele
- Subjects
SUSTAINABLE agriculture ,ROOT crops ,SUGAR beets ,PHYTOPATHOGENIC microorganisms ,AGRICULTURE ,BIOLOGICAL pest control agents ,PLANT parasites - Abstract
The importance of crop-associated microbiomes for the health and field performance of plants has been demonstrated in the last decades. Sugar beet is the most important source of sucrose in temperate climates, and-- as a root crop--yield heavily depends on genetics as well as on the soil and rhizosphere microbiomes. Bacteria, fungi, and archaea are found in all organs and life stages of the plant, and research on sugar beet microbiomes contributed to our understanding of the plant microbiome in general, especially of microbiome-based control strategies against phytopathogens. Attempts to make sugar beet cultivation more sustainable are increasing, raising the interest in biocontrol of plant pathogens and pests, biofertilization and --stimulation as well as microbiome-assisted breeding. This review first summarizes already achieved results on sugar beet-associated microbiomes and their unique traits, correlating to their physical, chemical, and biological peculiarities. Temporal and spatial microbiome dynamics during sugar beet ontogenesis are discussed, emphasizing the rhizosphere formation and highlighting knowledge gaps. Secondly, potential or already tested biocontrol agents and application strategies are discussed, providing an overview of how microbiome-based sugar beet farming could be performed in the future. Thus, this review is intended as a reference and baseline for further sugar beet-microbiome research, aiming to promote investigations in rhizosphere modulation-based biocontrol options. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
46. Importance of Lactic Acid Bacteria as an Emerging Group of Plant Growth-Promoting Rhizobacteria in Sustainable Agroecosystems
- Author
-
Mohammad Yaghoubi Khanghahi, Sabrina Strafella, Pasquale Filannino, Fabio Minervini, and Carmine Crecchio
- Subjects
biofertilization ,bioprotection ,plant growth-promoting rhizobacteria ,phytostimulation ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Increasing awareness of the problems caused by synthetic agrochemicals, such as chemical fertilizers, pesticides, and herbicides, makes it crucial to discover substitute approaches that can guarantee competitive plant production and protect the environment while maintaining the natural balance in agroecosystems. One of the leading alternatives is utilizing rhizobacterial strains named plant growth-promoting rhizobacteria (PGPR). The utilization of PGPR-based biofertilizers for advancement in the sustainability of farming productions has received considerable critical attention all over the world because of their contribution to not only improving plant growth but also inducing biotic and abiotic stress tolerance. This review updates the aforementioned eco-friendly strategy in sustainable agroecosystems and provides new insights into the phytostimulation and bioprotection ability of lactic acid bacteria (LAB), an emerging taxon of PGPR. In this regard, the ability of LAB to synthesize metabolites, including organic acids, phenolic acids and their flavonoid derivatives, phytohormones, and antimicrobial substrates, is presented. The use of LAB provides a bridge between PGPR and environmentally friendly crop productivity, which can lead to sustainable production systems by reducing the use of agrochemicals, improving soil quality, and minimizing environmental pollution. All the beneficial aspects of LAB need to be addressed by future research to plan systematic methodologies for their use and/or to combine the use of PGPR along with other organic or inorganic inputs in sustainable production systems.
- Published
- 2024
- Full Text
- View/download PDF
47. The Potential of Plant Growth-Promoting Rhizobacteria (PGPR) as Biological Tools in Enhancing Agricultural Sustainability
- Author
-
Malik, Garima, Chugh, Samira, Madan, Akanksha, Gupta, Vijai Kumar, Series Editor, Tuohy, Maria G., Series Editor, Sayyed, R.Z, editor, Singh, Anjana, editor, and Ilyas, Noshiin, editor
- Published
- 2022
- Full Text
- View/download PDF
48. EFFECT OF SOME SALINITY AND FERTILIZATION TREATMENTS ON BERMUDA: A. VEGETATIVE GROWTH
- Author
-
M.A.H. Abdou, M. Aly, and H. Ammar
- Subjects
cynodon dactylon ,salinity ,mineral fertilization ,biofertilization ,vegetative growth ,Plant ecology ,QK900-989 - Abstract
This study was undertaken at the private Farm, Bani Mazar District, Minia governorate. during the two seasons of 2020 and 2021 to investigate the effect of irrigation water salinity, mineral and biofertilization [effective microorganisms (EM) and Azotobacter chroococcum bacteria (AC)] treatments, and, their interactions on the vegetative growth of bermudagrass (Cynodon dactylon, L.), grown in sandy soil. Our results indicated that the vegetative growth traits (covering density, plant height, as well as, fresh and dry weights of clipping) were increased with the low level of salinity (3000 and 6000 ppm), while, they were decreased with the high level of salinity (9000 ppm) comparing with control treatment, with significant differences in some cases, in the three cuts during both seasons. The mineral and biofertilization treatments significantly increased the previous parameters compared with the control treatment, except some treatments (EM or AC) in 2nd and 3rd cuts with the highest values which were obtained due to 100% mineral NPK followed by biofertilizer (EM + AC), without significant differences in some cases, in the three cuts during both seasons. The interaction treatments were significant for all vegetative growth traits in the three cuts during both seasons. The best interaction treatments that mitigate the adverse effects of salinity (9000 ppm) were 100% mineral NPK followed by biofertilizer (EM + AC)
- Published
- 2022
- Full Text
- View/download PDF
49. EFFECT OF SOME SALINITY AND FERTILIZATION TREATMENTS ON BERMUDA: B. ROOT GROWTH AND SOME CHEMICAL COMPOSITION
- Author
-
M.A.H. Abdou, M. Aly, and H. Ammar
- Subjects
cynodon dactylon ,salinity ,mineral fertilization ,biofertilization ,root growth ,chemical composition ,Plant ecology ,QK900-989 - Abstract
This study was undertaken at the private Farm, Bani Mazar District, Minia governorate. during the two seasons of 2020 and 2021. The aim of this study was to investigate the effect of irrigation water salinity, mineral and biofertilization [effective microorganisms (EM) and Azotobacter chroococcum bacteria (AC)] treatments, as well as, their combinations on the root growth and some chemical constituents of bermudagrass (Cynodon dactylon, L.), grown in sandy soil. Results showed that the root growth parameters i.e., root length and fresh and dry weights/unit were enhanced with the low and medium levels of salinity (3000 and 6000 ppm), while, they decreased with the high level of salinity (9000 ppm) compared with control, in the third cut during both seasons. On the other hand, all salinity treatments increased Na, Cl, Ca (% in the dry herb) and proline content (in fresh weight), and decreased photosynthetic pigments as well as NPK %. All used mineral and/or biofertilization treatments significantly increased root length and fresh and dry weights/unit comparing with control treatment in 3rd cut, except AC for root length and EM and AC in case of fresh and dry weights/unit, with the highest values that were obtained due to 100% mineral NPK followed by EM + AC during both seasons. N, P, K and Ca % as well as photosynthetic pigments and proline content were increased due to application with any of the sub-plot treatments, while, Na and Cl were decreased. The best interaction treatments which mitigate the adverse effects of salinity (9000 ppm) were 100% mineral NPK followed by biofertilizer (EM + AC)
- Published
- 2022
- Full Text
- View/download PDF
50. Integrated biofertilization using yeast with cyanobacteria on growth and productivity of wheat
- Author
-
Seham M. Hamed, Naayem Mohamed El-Gaml, and Sherif Thabet Eissa
- Subjects
Cyanobacteria ,Yeast ,Biofertilization ,Wheat ,N2–fixation ,Yield components ,Medicine (General) ,R5-920 ,Science - Abstract
Abstract Background The heavy use of conventional mineral fertilization considerably increased wheat growth and yield components. However, the excessive nitrogen fertilization accounts for large expenses on farmers’ budget and has negative environmental drawbacks to soil and water ecosystems. Recently, establishing wheat plants associations with a variety of N2-fixing cyanobacteria and/or growth promoting microorganisms in farming systems as nutrients source is seen as ecofriendly and economically feasible solution. Results In this work, the influence of different nitrogen (N) levels (100% N, 75% N, and 50% N as urea) and cyanobacteria (Cy) and/or yeast (Y) inoculation each alone or both in combination with different nitrogen levels were assessed on two different wheat (Triticumae stivum L.) genotypes (Sids-14 and Giza-171) through field experiments over two consecutive seasons (2019/2020 and 2020/2021). Although, the full recommended dose (FRD) (100% N) showed the highest chlorophyll (Chl) a, b content during tillering stage, grains quality (NPK-uptake, protein%), and wheat yield components in both genotypes. The integrated inoculation of cyanobacteria and yeast with 50% N, 75% N-fertilization enhanced wheat growth characteristic (Chl a,b) and upgraded soil microbial community (nitrogenase activity and CO2 evolution) in Sids-14 and Giza-171. Cy combined with Y and partial N-fertilization levels enhanced NPK-uptake Kg/fed and protein% in wheat grains in the two wheat genotypes. Moreover, this combination recorded a wheat yield components (plant height, number of spikes/m2, number of grains/spike, 1000 grains wt (gm) and grains yield (tonne/fed) insignificantly different from those obtained by FRD in wheat cultivation. Conclusion The integrated application of cyanobacteria with yeast and 50% N or 75% N-fertilization improved soil fertility index and promoted NPK- uptake, protein% and wheat yield components, showing comparable values to conventional chemical fertilization. Cyanobacteria combined with yeast inoculation had socio-economic benefits as it can save about 25–50% of the required mineral nitrogen fertilizers for wheat crop production.
- Published
- 2022
- Full Text
- View/download PDF
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