154 results on '"Blagojević, Veljko"'
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2. Basic characteristics of Albanian strategic culture
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Blagojević Veljko B.
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albania ,strategic culture ,national values ,foreign policy ,national security ,war ,Military Science - Abstract
Throughout history, Serbs and Albanians have had periods of coexistence and cooperation, but more often than not there have been periods of conflicts, wih the long-standing one regarding the control over the territory of Kosovo and Metohija. Nevertheless, almost half a century since the appearance of the concept, there have been no significant efforts to define basic characteristics of Albanian strategic culture, which are evident shortcomings of Serbian strategic studies. This paper has the aim to initiate a process of systematic study of the abovementioned research problem, with the aim to objectively and rationally obtain basic characteristics of Albanian strategic culture by applying almost all scientific methods, especially hypothetico-deductive method. The paper's main hypothesis states as follows: basic characteristics of Albanian strategic culture are predominantly "mercantile" approach towards diplomacy with strong support from diaspora, by which they seek to support persistent aspirations for the control over the territory with the majority of Albanian population, whereby they do not hesitate to use political violence, while the military force is engaged exclusively with previously provided support from great powers. Basic characteristics of Albanian strategic culture point to the necessity to reevaluate our strategic approach towards Albanians.
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- 2023
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3. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans
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Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, and Minić, Rajna
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- 2022
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4. Evaluation of the Anti-Inflammatory/Immunomodulatory Effect of Teucrium montanum L. Extract in Collagen-Induced Arthritis in Rats.
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Bufan, Biljana, Marčetić, Mirjana, Djuretić, Jasmina, Ćuruvija, Ivana, Blagojević, Veljko, Božić, Dragana D., Milutinović, Violeta, Janković, Radmila, Sopta, Jelena, Kotur-Stevuljević, Jelena, and Arsenović-Ranin, Nevena
- Subjects
EXPERIMENTAL arthritis ,COLLAGEN-induced arthritis ,TREATMENT effectiveness ,ANKLE joint ,ORAL drug administration ,B cells - Abstract
Simple Summary: The use of medicinal traditional plants and plant-derived compounds in the treatment of inflammatory states, including rheumatoid arthritis, is gaining attention due to their anti-inflammatory and immunomodulatory properties and fewer side effects compared to conventional drugs. The current study aimed to evaluate the therapeutic effects of the Mediterranean plant Teucrium montanum L. on collagen-induced arthritis in rats, in an in vivo animal model of rheumatoid arthritis. The effect of the Teucrium montanum extract was evaluated by examining the cellular and molecular components of innate and adaptive immunity that have been shown to play an important role in the pathogenesis of both rheumatoid arthritis and collagen-induced arthritis. The Teucrium montanum extract alleviated the clinical manifestation and improved the histopathological findings of arthritis in CIA. The extract improved the anti-/pro-oxidative balance in serum, suppressed the production of pro-inflammatory cytokines in affected joints, and suppressed the T cell responses in secondary lymphoid organs and the production of autoantibodies. Our results suggest the anti-inflammatory/immunomodulatory properties of Teucrium montanum extract and represent a basis for the further exploration of the possible therapeutic use of the extract or its compound/s. The anti-inflammatory/immunomodulatory effects of Teucrium montanum L. (TM), a plant distributed in the Mediterranean region, have been insufficiently examined. The effects of the TM ethanol extract were tested in a rat collagen-induced arthritis (CIA) model of rheumatoid arthritis. LC-MS was used for the phytochemical analysis of the TM extract. Dark Agouti rats were immunized with bovine type II collagen (CII) in incomplete Freund's adjuvant for CIA, and treated with 100 or 200 mg/kg of TM extract daily via oral administration. Clinical and histopathological evaluations and a flow cytometric analysis of the phenotypic and functional characteristics of splenocytes and draining lymph node cells were performed. The cytokines in the paw tissue culture supernatants and anti-CII antibodies in serum were determined by ELISA. The TM extract, with the dominant components verbascoside and luteolin 7-O-rutinoside, reduced the arthritic score and ankle joint inflammation in CIA rats, promoted the antioxidant profile in serum, and lowered pro-inflammatory TNF-α, IL-6 and IL-1β production. It suppressed the activation status of CD11b+ cells by lowering CD86, MHCII and TLR-4 expression, and promoted the Th17/T regulatory cell (Tregs) balance towards Tregs. A lower frequency of B cells was accompanied by a lower level of anti-CII antibodies in treated rats. These findings imply the favorable effect of TM extract on the clinical presentation of CIA, suggesting its anti-inflammatory/immunomodulatory action and potential therapeutic effect. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Strategic deterrence: The key competences of the state bodies of the Republic of Serbia
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Blagojević Veljko and Radanović Tihomir
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strategic deterrence ,national security system ,competences of institutions ,preventive action ,Military Science - Abstract
In the post-Cold War period, contemporary studies of strategic deterrence have expanded from traditional ones to contents that are much broader in terms of content and topics. It can be said that today this is the comprehensive activity that should engage the entire society, proclaiming the concept of the whole power. Providing the systematic implementation of the principle of comprehensiveness, together with the principle of knowledge-worthiness and timeliness, certainly represents a key prerequisite for effective deterrence in modern conditions. Military power certainly represents a fundamental capability for deterrence in the context of support to foreign policy, diplomatic efforts and economic cooperation with foreign countries, as well as in terms of psychological and informational resistance. Another great problem is which state authority to entrust with the implementation of strategic deterrence. It seems most acceptable for the National Security Council to assume the responsibility for the implementation of strategic deterrence, with the necessary organizational and functional changes in terms of recruiting personnel for the Council. The paper deals with the problem how to distribute the competences of state authorities in the implementation of deterrence. We believe that this paper has offered quite enough arguments that it is necessary to legally regulate this field in a manner that it changes the basic assumptions of the competences of state authorities to the least possible extent. It is clear that this is a conceptual model that requires further elaboration and concretization, but it can serve as a starting point for further research.
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- 2022
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6. Modulation of T-cell-dependent humoral immune response to influenza vaccine by multiple antioxidant/immunomodulatory micronutrient supplementation
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Bufan, Biljana, primary, Arsenović-Ranin, Nevena, additional, Živković, Irena, additional, Ćuruvija, Ivana, additional, Blagojević, Veljko, additional, Kotur-Stevuljević, Jelena, additional, and Leposavić, Gordana, additional
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- 2024
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7. Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity
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Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, and Vujić, Vesna
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- 2021
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8. DESCRIPTION OF A NEW POTENTIAL AGGREGATION FACTOR FROM THE STREPTOCOCCUS THERMOPHILUS GENOME
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Tsibulskaya, Darya, Blagojević, Veljko, Terzić-Vidojević, Amarela, Lukić, Ivana, Vasić, Marko, Dragačević, Luka, Kojić, Milan, Tsibulskaya, Darya, Blagojević, Veljko, Terzić-Vidojević, Amarela, Lukić, Ivana, Vasić, Marko, Dragačević, Luka, and Kojić, Milan
- Abstract
Autoaggregation, the ability to self-aggregate, is widespread among both Gram-positive and Gram-negative bacteria. The functional role of aggregation is not fully understood, but it is believed to be involved in the adaptation of bacteria to environmental conditions (PMID: 31294207). One interesting class of compounds responsible for the aggregation of lactic acid bacteria is aggregation factors—surface high-molecular-weight proteins rich in threonine and lysine (PMID: 30027759). Recently, our research group discovered a new strain of Streptococcus thermophilus in the mountainous regions of Serbia, exhibiting an aggregation phenotype. Aggregation phenotype was confirmed visually and using microscopy. Complete genome of Agg+ strain was sequenced using NGS and a gene encoding a potential aggregation factor, which was named aggS was identified. The predicted threonine (12.5%) and lysine (10.5%) rich protein contains 2367 amino acids, with an average molecular weight of 255986.63 Da. AggS also contains two cysteine residues,whereas previously well-described aggregation factors of this type did not contain any cysteine residues. The predicted protein includes an N-terminal YSIRK-like signal sequence and an LPXTG cell wall anchor domain. It has 6 Mucin binding domain repeats alternating with 6 Mub B2-like domain repeats. Additionally, we found a region resembling an ice-binding domain. Given that these bacteria endure prolonged periods of low temperatures, it can be speculated that this surface membrane protein also helps the bacteria withstand freezing. The fact that the alignment using BLASTp revealed AggS to be most closely related to an uncharacterised protein from the genome of Lactococcus garvieae, along with the discovery of a transposase gene sequence upstream of the gene, suggests that the aggregation factor was likely acquired through horizontal gene transfer. We plan to clone it into a shuttle vector and investigate the aggregation phenotype using a heterologou
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- 2024
9. Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom
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Lukić, Ivana, Blagojević, Veljko, Minić, Rajna, Ivanović, Saša, Borozan, Sunčica, Ćupić, Vitomir, Živković, Irena, Lukić, Ivana, Blagojević, Veljko, Minić, Rajna, Ivanović, Saša, Borozan, Sunčica, Ćupić, Vitomir, and Živković, Irena
- Abstract
that can most closely evaluate the biological activity of the drug of interest. For testing the potency of antivenom, these are the methods used to assess cytotoxicity. The aim of this study was to evaluate the most commonly used cytotoxicity methods for determining the protective potency of the antivenom Viekvin, which neutralizes Vipera ammodytes venom. Material and methods: The selected methods are based on different biological mechanisms: MTT assay, based on the activity of cell oxidoreductase enzymes; crystal violet staining, based on the degree of cell adhesion; trypan blue staining, based on cell membrane permeability, and propidium iodide staining, based on measurement of nucleic acids of dead cells. The pro-apoptotic effect of the venom was also determined with annexin V staining. Results: The IC50 value of V. ammodytes venom obtained by these methods was very similar, while the EC50 values differed significantly. Conclusions: We concluded that the choice of the method used to measure the anticytotoxic antivenom potency depends on the immunogenicity of the venom components that cause cell death; for each venom/antivenom pair, it is necessary to select the appropriate assay separately, and at present, none of the standard cytotoxic methods can be universally applied to determine antivenom potency.
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- 2024
10. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
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Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljevic, Milica, Janković, Radmila, Djuretić, Jasmina, Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljevic, Milica, Janković, Radmila, and Djuretić, Jasmina
- Abstract
Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
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- 2024
11. NMDA Receptor Antagonist Memantine AmelioratesExperimental Autoimmune Encephalomyelitis in Aged Rats
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Bufan, Billjana, Bufan, Billjana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, Đuretić, Jasmina, Bufan, Billjana, Bufan, Billjana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, and Đuretić, Jasmina
- Abstract
Aging is closely related to the main aspects of multiple sclerosis (MS). The average age ofthe MS population is increasing and the number of elderly MS patients is expected to increase. Inaddition to neurons,N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronalcells, such as immune cells. The aim of this study was to investigate the role of NMDARs inexperimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and agedDark Agoutirats from day7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease,reactivation, and apoptosis of CD4+T cells in the target organ of aged EAE rats. The expression ofthe fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent inaged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expressionin brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advancedoxidation protein products in brain tissue were consistent with previous results. Overall, our resultssuggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
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- 2024
12. THE CONCEPT OF DETERRENCE IN CURRENT RELATIONS IN THE MIDDLE EAST.
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MILOSAVLJEVIĆ, branislav and BLAGOJEVIĆ, Veljko
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IRAN-United States relations ,GREAT powers (International relations) ,NON-state actors (International relations) ,INTERNATIONAL relations ,COLD War, 1945-1991 - Abstract
Copyright of Review of International Affairs (04866096) is the property of Institute of International Politics & Economics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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13. Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation.
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Bufan, Biljana, Arsenović-Ranin, Nevena, Živković, Irena, Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Kovačević, Ana, Kotur-Stevuljević, Jelena, and Leposavić, Gordana
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FLU vaccine efficacy ,HUMORAL immunity ,GERMINAL centers ,ANTIBODY formation ,VACCINE effectiveness - Abstract
Notwithstanding prevalence gaps in micronutrients supporting immune functions, the significance of their deficits/supplementation for the efficacy of vaccines is underinvestigated. Thus, the influence of supplementation combining vitamins C and D, zinc, selenium, manganese, and N-acetyl cysteine on immune correlates/surrogates of protection conferred by a quadrivalent influenza vaccine (QIV) in mice was investigated. The supplementation starting 5 days before the first of two QIV injections given 28 days apart increased the serum titres of total and neutralizing IgG against each of four influenza strains from QIV. Accordingly, the frequencies of germinal center B cells, follicular CD4+ T helper (Th) cells, and IL-21-producing Th cells increased in secondary lymphoid organs (SLOs). Additionally, the supplementation improved already increased IgG response to the second QIV injection by augmenting not only neutralizing antibody production, but also IgG2a response, which is important for virus clearance, through favoring Th1 differentiation as indicated by Th1 (IFN-γ)/Th2 (IL-4) signature cytokine level ratio upon QIV restimulation in SLO cell cultures. This most likely partly reflected antioxidant action of the supplement as indicated by splenic redox status analyses. Thus, the study provides a solid scientific background for further research aimed at repurposing the use of this safe and inexpensive micronutrient combination to improve response to the influenza vaccine. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Military cliques in the Serbian strategic culture of the 20th century
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Blagojević Veljko and Petrović Dejan
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military cliques ,military organization ,political elite ,strategic culture ,professionalism ,Military Science - Abstract
Throughout the entire 20th century, the Serbian strategic culture was "torn" by various discontinuities, from the state system, through foreign policy and ideological, to strategic and doctrinal orientation, and it can be said that discontinuity is almost its only constant. Nevertheless, the existence of military cliques can be considered to be one of the constants of the Serbian strategic culture. They followed the Serbian Army and influenced the political life of the Kingdom of Serbia. The dynastic coup of May 1903 can be taken as an example of their actions. After that, younger conspirators established the so-called Black Hand, whose leader was Colonel Apis, who was shot after the Thessaloniki process in 1917. The interwar period was marked by the action of the so-called White Hand, under the leadership of General Petar Živković, which was active until the assassination of King Alexander Karađorđević in Marseilles in 1934. The Second Yugoslavia even had a formalized "military clique" represented in the League of Communists, which was an integral part of the League of Communists of Yugoslavia at federal level, together with the republican leagues. Thus, the influence of the party on the military organization was secured, and the influence of the army in the society was also "secured" by its representation in the party forum at federal level. Consequently, the Serbian political elite welcomed the dissolution of the SFRY with perhaps the only constant in its strategic culture, and that is the reliance on the military in resolving political crises, which can be vividly presented by the statement of Slobodan Milosević: "We do not know how to work, but we know how to fight".
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- 2021
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15. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
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Bufan, Biljana, primary, Ćuruvija, Ivana, additional, Blagojević, Veljko, additional, Grujić-Milanović, Jelica, additional, Prijić, Ivana, additional, Radosavljević, Tatjana, additional, Samardžić, Janko, additional, Radosavljevic, Milica, additional, Janković, Radmila, additional, and Djuretić, Jasmina, additional
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- 2024
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16. Dragutin Dimitrijević Apis as a symbol of the Serbian strategic culture
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Blagojević Veljko
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apis ,black hand ,the karađorđević dynasty ,strategic culture ,serbian political and legal system ,Military Science - Abstract
The paper is the analysis of the life and achievements of Colonel Dragutin Dimitrijević Apis, an army and intelligence officer, who significantly influenced the history of Serbs at the beginning of the 20th century. The turbulent, almost adventurous, life of Colonel Apis ended with the rigged trial in Thessaloniki in 1917. From then until the present day, Apis is used as a symbol of the Serbian strategic culture for the military involvement in politics, but also as a patriotic and self-sacrificing officer dependent on the needs of the ruling elite and the facts which subculture analysts belong to. The aim of this paper is to analyze the achievements of Colonel Apis in an analytical and objective way, to describe different strategic subcultures in Serbia on the example of the attitude towards the symbol of Apis and to remove certain misconceptions about the (mis)use of Colonel Apis and his legacy.
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- 2020
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17. The specificity of implementing the Vienna Convention on Diplomatic Relations in defense (military) diplomacy
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Blagojević Veljko
- Subjects
diplomacy ,defence diplomacy of the republic of serbia ,diplomatic law and practice ,vienna convention on diplomatic relations ,protocol ,Law - Abstract
The Vienna Convention on Diplomatic Relations regulates the general framework for the implementation of diplomatic functions of State. Contemporary diplomacy is evolving in many areas that have not traditionally been in its focus, such as police and intelligence cooperation. The Republic of Serbia has also started appointing police and Security-Intelligence Agency representatives to diplomatic missions abroad. It is essential to monitor and study this practice from the standpoint of legal regulation. This matter is regulated by diplomatic and consular law, but also by national legislation and diplomatic practice. The experience and tradition of defense (military) diplomacy of the Republic of Serbia in adapting its functions to the Vienna Convention on Diplomatic Relations, legal regulation and organizational and functional support to the defense attachés network, the procedures for selecting defense attachés, training and preparing them for the duty, as well as monitoring and directing them during the performance of the diplomatic service are indisputable at the national level. The tradition of military diplomacy has prevailed for more than 140 years, which indicates the level of diplomatic practice that leads to the conclusion that Serbia does have all the needed criteria for quality and functional implementation of the assigned tasks. The question is how these criteria are met in the newly formed network of diplomatic representations of the Police and the Security-Information Agency, bearing in mind the extremely negative experiences with economic representatives of the Republic of Serbia abroad. There is no doubt that their engagement is in line with international and national legislation, but the issue of the lack of diplomatic practice in organizational and functional terms remains a questionable one. Furthermore, there is the issue of their responsibilities within the diplomatic and consular missions and the harmonization of different professional cultures and ethics, as well as the preparation for performing their duties abroad. It is clear that all these problems cannot be solved in a short term, which is a relatively new organizational solution. Some time is needed to detect shortcomings in practice and generate the experience necessary to overcome them. The practice of organizing defense (military) diplomacy of the Republic of Serbia can certainly serve as a signpost for their resolution, while respecting the specificities arising from different responsibilities and professional ethics. By analyzing the specifics of the implementation of the Vienna Convention on Diplomatic Relations in defense (military) diplomacy, analogies can be seen with the defining functions, while its legal and organizational experiences can serve as a guide to regulating the functions of Police and Security-Intelligence Agency representatives in the diplomatic service.
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- 2020
18. Noradrenaline through β-adrenoceptor contributes to sexual dimorphism in primary CD4+ T-cell response in DA rat EAE model?
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Vujnović, Ivana, Pilipović, Ivan, Jasnić, Nebojša, Petrović, Raisa, Blagojević, Veljko, Arsenović-Ranin, Nevena, Stojić-Vukanić, Zorica, Djordjević, Jelena, and Leposavić, Gordana
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- 2019
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19. Strateška kultura - studija slučaja - Srbija
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Blagojević Veljko
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Military Science - Abstract
nema
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- 2019
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20. Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis
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Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Kotur-Stevuljević, Jelena, Bogdanović, Andrija, Petrović, Raisa, Vujnović, Ivana, and Kovačević-Jovanović, Vesna
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- 2018
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21. Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?
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Blagojević, Veljko, Kovačević-Jovanović, Vesna, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, Ivana, Vujić, Vesna, and Stanojević, Stanislava
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- 2018
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22. Ključne reperkusije migracione krize u jugoistočnoj Evropi
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Blagojević Veljko and Pejić Igor
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Military Science - Abstract
nema
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- 2018
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23. Od ustanka do međunarodnog priznanja - vojska u stvaranju državnog identiteta Kneževine Srbije
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Blagojević Veljko and Pejić Igor
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Military Science - Abstract
nema
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- 2018
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24. Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom
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Milovanović, Vladimir, Blagojević, Veljko, Lukic, Ivana, Minić, Rajna, Borozan, Sunčica, Ćupić, Vitomir, Ivanović, Saša, Živković, Irena, Milovanović, Vladimir, Blagojević, Veljko, Lukic, Ivana, Minić, Rajna, Borozan, Sunčica, Ćupić, Vitomir, Ivanović, Saša, and Živković, Irena
- Abstract
Introduction: In recent decades, many efforts have been made in laboratories to develop alternative methods that would replace animal tests or reduce the number of animals used. In this sense, the methods for measuring cytotoxicity have proven to be very suitable substitutes for testing toxicity. As each individual substance being tested has its own specificities, it is necessary to carefully select a specific, appropriate cytotoxic method. This is especially important if the investigated substance is complex, such as animal venom. Also, antivenoms are drugs for which it is necessary to correctly determine the protective power.Methods: This study aims to evaluate most commonly methods for evaluating citotoxiciy of Vipera ammodytes venom and protective potency of appropriate antivenom. For this purpose, methods based on different biological mechanisms: MTT enzyme assay, Crystal Violet (CV), Trypan Blue (TB) and Propidium Iodide (PI) staining, were examined on Vero cells. The proapoptotic effect of the venom was also determined with AnexinV staining.Results: The IC50 value of V. ammodytes venom evaluated by MTT, CV, TB, PI (%) methods was similar: 62.5, 55, 66, 87 g/ml, respectively, and the most suitable methods for testing the cytotoxicity of V. ammodytes venom were methods based on the measurement of living cells, which were MTT and CV staining. The same methods were used to test the anticytotoxic potency of the antivenom and each method confirmed significant anticytotoxic potential, but the EC50values obtained by those methods varied significantly.Conclusion: Choice of the method used to measure the anticytotoxic antivenom potency depends on the immunogenicity of the venom components that cause cell death.
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- 2023
25. Serbian diplomacy in deterrence
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Blagojević, Veljko, primary
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- 2023
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26. Political and legal framework of contemporary migrations in southeast Europe
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Blagojević Veljko and Milosavljević Branislav
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migration ,asylum ,refugee ,balkan migration route ,migration policy ,Military Science - Abstract
The phenomenon of human migrations is as old as the civilization. The reasons why people choose to change their place of residence are complex and subject to frequent changes. That is why the classification of migration is a very complex matter, both for legal and security theorists. However, international legal regulations have been adopted, which provide rights for migrants. On the other hand, the given rights to freedom of movement and protection from persecution are limited by state sovereignty, as historical, legal and political category. When supranational international organizations, such as the European Union and other regional organizations, are added to national sovereignty, the difficulty of legal regulation of that matter can be understood. Countries in the region are trying to adapt to circumstances arising from mass migrations through their respective territories, where each is led primarily by political decisions based on national interests, in the absence of a broader, European migration policy that would be a comprehensive effort to solve the afore mentioned problem. The Southeast European region is a transit hub for smuggling, trafficking and illegal migrations in general, which requires further caution of competent authorities when classifying certain categories of migrants and, therefore, the compliance with international and domestic standards regarding their guaranteed rights. The aim of this paper is to analyze global and regional migrations and their future trends, with a focus on South East European region, as well as to establish basic motives that cause migration processes and their classification, which could be used for the proper treatment of migrants in accordance with their rights and the respective national legislation.
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- 2016
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27. Organized crime in Western Balkans: Case Serbia
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Štrbac Katarina, Milosavljević Branislav, and Blagojević Veljko
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organized crime ,criminal law ,methods of undercover investigation ,international cooperation ,Military Science - Abstract
This paper discusses the common heritage of the Western Balkans in connection with organized crime, which, it can be claimed, did not significantly manifest under socialism. The disintegration of the former Yugoslavia led to war, destruction, and overall increase in crime, including organized crime. The factors that especially contributed to that, besides the war, are: the transition process, the increase in the level of corruption, inefficient functioning of the state apparatus, economic decline, and trade sanctions imposed by the United Nations which resulted in a sort of semi-legal cooperation between governmental authorities and organized criminal groups in the Western Balkans. Local wars in the former Yugoslavia enabled the expansion of illegal arms trafficking, theft and smuggling of motor vehicles, and smuggling of all kinds of excise goods, especially oil and cigarettes. The trafficking of narcotics became a very lucrative and widespread activity of criminal groups, with the geo-strategic position of the Western Balkans countries being one of the contributing factors. The beginning of democratic changes in Serbia marked the commencement of the fight against organized crime. It was also the start of the creation of the legal regulatory system to counter organized crime, which involves interstate cooperation at both the regional and global level, as well as the adoption of international conventions and standards. Consequently, a special police unit for combating organized crime was established within the Serbian Ministry of Interior. The Republic of Serbia, like most countries in the region, has adopted a series of legislative measures to combat organized crime, which come from experiences of other countries that have shown good results. Specific aspects of the investigative procedure and collecting evidence of organized crime cannot be achieved using conventional methods of investigation, such as home, other premises and persons’ search warrants, temporary confiscation of items, questioning of the accused, witnesses’ hearing, etc. In these efforts the basic dilemma is the level of proportionality between security and freedom, because the methods of an undercover investigation are special methods that are used to temporarily restrict the fundamental rights and freedoms of men, for the purposes of combating complex forms of crime.
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- 2016
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28. Indicators of the existence and operations of organized crime
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Milosavljević Branislav and Blagojević Veljko
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threat to security ,organized crime ,forms of manifestation ,prevention ,indications ,indicators ,Military Science - Abstract
In security sciences, indicators have a dual purpose – as indicators of the existence and operation of various forms of threats, and as an evaluation of the security subjects’ performance in combating the criminal activity. In professional literature, the terms indicators and indications are often identified, and erroneously understood as synonymous. However, these are different logical notions, with a completely different application in practice. The paper discusses basic issues related to the indicators of the security threat manifestations. Apart from general considerations, special attention is paid to indicators of organized crime. The authors analyze the process of defining the indicators of the existence and operations of organized crime, which is determined by specific features of this type of security threat and also by the specialization of certain government entities that have been put in charge of countering the organized crime. The determination of the indicators arises from the obligation of government authorities to have elaborated indicators in accordance with their assigned responsibilities in the fight against organized crime, and other forms of threats as well. Also discussed are some indicators that represent a solid basis for further upgrade in line with the specific characteristics and changes in the manifestation of this form of threat.
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- 2016
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29. Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression
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Ćuruvija, Ivana, Stanojević, Stanislava, Arsenović-Ranin, Nevena, Blagojević, Veljko, Dimitrijević, Mirjana, Vidić-Danković, Biljana, and Vujić, Vesna
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- 2017
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30. Why war, not an International congress in 1914?
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Blagojević Veljko and Stojanović Stanislav
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diplomacy ,international order ,national interests ,balance of power ,world war one ,Military Science - Abstract
The paper discusses the key developments and processes preceding the World War I, and answers the question of why the then political elites had not opted for holding an international conference where the interests of the major powers could have been agreed by compromise according to the diplomatic model of the Holy Alliance. The analysis of the causes of the failure of diplomacy to prevent the outbreak of the Great War is based on consideration of the characteristic of diplomatic practice of the so-called Concert of Europe, the influence of Germanic factor on antagonizing the relations between the European powers, the process of emancipation of Serbia and its diplomacy, as well as the domination of militaristic perception in the summer of 1914.
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- 2015
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31. Intelligence services as agents of a coup
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Milenković Miloš and Blagojević Veljko
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intelligence services ,subversive activity ,overthrow ,coup ,Military Science - Abstract
Intelligence services, as part of the state apparatus that is basically designed to collect important information needed for decision-making, represent also an important tool for states to achieve strategic interests beyond its borders. In achieving these strategic objectives imposed on them by the government, intelligence services conduct different types of operations abroad, even those that are not solely of intelligence nature. Such actions of intelligence services are called subversive or non-intelligence actions, and are carried out through various forms, among which is an overthrow. The subject of this paper is overthrows that are accomplished by execution of a coup, in which intelligence services can have, and usually do have, their place and role. In practice, a coup is usually prepared and executed by such more powerful intelligence services that are both organizationally and financially capable of this type of complex action. As one of agents of a coup, intelligence services are involved in all phases of its implementation. Their role, although important for the ultimate success of the coup, usually remains insufficiently known to the general public, because of the nature of their activities. Nevertheless, it is possible to identify methods by which intelligence services exercise their role of agents of the coup.
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- 2015
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32. The strategic context of World War I
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Stojanović Stanislav and Blagojević Veljko
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world war i ,strategic context ,the european international order ,interests ,strategies ,war plans ,Military Science - Abstract
The paper discusses the circumstances that had been decisive for the creation of a strategic context for the outbreak of World War I. It analyzes the political, economic and strategic interests of key actors in European international order in the late 19th and early 20th century, and how they led to the polarization of European politics and the formation of opposing alliances. Also, the main ideas of their war plans are discussed, as well as the aggressive positioning of Germany and Austro-Hungary before the commencement of the Great War. Special attention is addressed to the geopolitical position of Serbia from after it had gained independence until the beginning of World War I, as well as the challenges faced by the Balkan region in the swirl of complicated international political relations in Europe by the late 19th and early 20th century.
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- 2015
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33. Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans
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Nikodijević, Slavomir, primary, Blagojević, Veljko, additional, Ćuruvija, Ivana, additional, Kosanović, Dejana, additional, Djukić, Tamara, additional, Djordjević, Brižita, additional, Ilić, Vesna, additional, and Minić, Rajna, additional
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- 2022
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34. Fenotipske karakteristike peritonealnih makrofaga dva soja pacova tokom razvoja inflamatornog odgovora: značaj predstavnika crevne mikrobiote
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Blagojević, Veljko, Stanojević, Stanislava, Božić Nedeljković, Biljana, Miljković, Đorđe, Minić, Rajna, and Mitić, Katarina
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AO pacovi, DA pacovi, imunomodulacija, inflamacija, komensalne bakterije, kolitis, Lactobacillus rhamnosus, makrofagi peritonealne šupljine ,AO rats, commensal bacteria, colitis, DA rats, immunomodulation, inflammation, Lactobacillus rhamnosus, peritoneal cavity macrophages - Abstract
Predmet istraživanja ove doktorske disertacije je ispitivanje promena u fenotipu i aktivnosti makrofaga peritonealne šupljine Albino Oxford (AO) i Dark Agouti (DA) pacova tokom razvoja inflamatornog odgovora koji je indukovan delovanjem bakterija mikrobiote creva direktno, intraperitonealnom (i.p.) primenom, ili indirektno, tokom razvoja kolitisa, kao i ispitivanje modulacije ovih promena primenom probiotske bakterije Lactobacillus rhamnosus (L. rhamnosus). Rezultati su pokazali da i.p. primena umrtvljenih E. coli ili Enterococcus spp. dovodi do izraženije inflamatorne polarizacije makrofaga peritonealne šupljine DA pacova u odnosu na AO soj, dok i.p. primena L. rhamnosus ima veći potencijal da spreči funkcionalne promene do kojih dovodi in vitro stimulacija komensalnim bakterijama u makrofagima pacova AO soja. Tokom razvoja kolitisa makrofagi DA pacova produkuju više IL-6 u odnosu na AO soj, a značajno manja smrtnost AO pacova je praćena intenzivnijom produkcijom IL-10 u makrofagima u odgovoru na dodatnu stimulaciju komensalnim bakterijama in vitro i višim nivoom antitela klase IgA specifičnih za E. coli u serumu. Rani postnatalni oralni tretman bakterijom L. rhamnosus u DA pacovima povećava raznovrsnost Bifidobacterium vrsta u fecesu, a nakon indukcije kolitisa u adultnom dobu smanjuje stepen ulceracije kolona, povećava zastupljenost rezidentnih makrofaga peritonealne šupljine i suprimira povećanja produkcije NO i IL-10 stimulisana komensalnim bakterijama in vitro. Nalazi ukazuju na značaj genetski determinisane predispozicije ka određenom obrascu aktivacije makrofaga za promene u fenotipu i aktivnosti tokom razvoja inflamatornog odgovora koji je indukovan delovanjem bakterija mikrobiote creva, kao i za modulaciju ovih promena primenom probiotske bakterije L. rhamnosus. The subject of this doctoral dissertation is the study of changes in the phenotype and activity of peritoneal cavity macrophages from Albino Oxford (AO) and Dark Agouti (DA) rats during the development of inflammatory response induced by intestinal microbiota directly, intraperitoneally (i.p.), or indirectly, through development of colitis, as well as study of the modulation of these changes using the probiotic bacterium Lactobacillus rhamnosus (L. rhamnosus). I.p. application of killed E. coli or Enterococcus spp. leads to a more pronounced inflammatory polarization of the peritoneal cavity macrophages from DA rats compared the AO strain, while i.p. application of L. rhamnosus has a greater potential to prevent functional changes caused by in vitro stimulation with commensal bacteria in AO rat macrophages. During the development of colitis, DA rat macrophages produce more IL-6 than the AO strain, and significantly lower AO rat mortality was accompanied by more intense IL-10 production in macrophages in response to additional stimulation with commensal bacteria in vitro and higher levels of IgA-class antibodies specific to E. coli in the serum. Early postnatal oral treatment with L. rhamnosus in DA rats increased the diversity of Bifidobacterium species in feces, and after induction of colitis in adulthood reduced the degree of colonic ulceration, increased the presence of resident peritoneal cavity macrophages and suppressed the increased production of NO and IL-10 stimulated by commensal bacteria . The findings indicate the importance of genetically determined predisposition to a certain pattern of macrophage activation for changes in phenotype and activity during the development of inflammatory response induced by intestinal microbiota, as well as for modulation of these changes using probiotic bacterium L. rhamnosus.
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- 2022
35. Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4
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Dimitrijević, Mirjana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujnović, Ivana, Petrović, Raisa, Arsenović-Ranin, Nevena, Vujić, Vesna, and Leposavić, Gordana
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- 2016
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36. Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.
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Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, and Minić, Rajna
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- 2022
37. Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation
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Blagojević, Veljko, Ćuruvija, Ivana, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, and Stanojević, Stanislava
- Abstract
Inflammation is a redistribution of immune cells, providing a more efficient elimination of the inflammatory offense. However, it is not limited to local microenvironment. In this study, the interaction of the effect of BCG priming and peritoneal inflammation on the remote inflammatory milieu of infected lung was investigated. Young male AO rats infected with Mycoplasma spp. were s.c. injected with BCG (3x105 CFU) or saline, and 7 days later received an i.p. injection of 7ml of thioglycollate (TG) or saline. Up to 7 days after TG injection, a broncho-alveolar lavage (BAL) was performed, and cells were analysed for their surface marker expression and NO production. Infected rats had a high percentage of HIS48HiCD11bHi neutrophils. BCG priming didn’t alter BAL cells phenotype, while TG injection increased the proportion of MHCII+CD11blow activated alveolar macrophages (aAMFs) on day 7. However, the BCG+TG group showed significant changes – percentage of HIS48HiCD11bHi neutrophils decreased from day 3, the share of aAMFs increased from day 5 and the share of MHCII+CD11b-AMFs increased on days 3-5. However, the percentage of B220+FSClow B lymphocytes were increased from day 1. Production of NO from BAL fluid cells was low in all groups. We conclude that BCG vaccination likely increased the number of circulating B lymphocytes, while TG-induced peritoneal inflammation potentially prevented their entry into the peritoneal cavity, forcing them into permissive tissues, such as lungs.
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- 2022
38. Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans
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Nikodijević, Slavomir, Nikodijević, Slavomir, Blagojević, Veljko, Ćuruvija, Ivana, Kosanović, Dejana, Đukić, Tamara, Đorđević, Brižita, Ilić, Vesna, Minić, Rajna, Nikodijević, Slavomir, Nikodijević, Slavomir, Blagojević, Veljko, Ćuruvija, Ivana, Kosanović, Dejana, Đukić, Tamara, Đorđević, Brižita, Ilić, Vesna, and Minić, Rajna
- Abstract
Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.
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- 2022
39. Supplementary information for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Applied Biochemistry and Biotechnology 2022, 194, 2047–2060. https://doi.org/10.1007/s12010-021-03772-w.
- Author
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Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, and Minić, Rajna
- Abstract
SDS-PAGE showing: Lane 1 – purified banana lectin – BanLec; Lane 2 – chimera of banana lectin and enhanced green fluorescent protein BanLec-eGFP; Lane 3 – MW – molecular weight markers; Table 1. Repeatability of flow cytometric detection of different quantities of BL-eGFP binding to C. albicans; Table 2. Repeatability of ELLSA detection of different quantities of BanLec-B binding to C. albicans; Table 3. Repeatability of flow cytometric detection of different quantities of RCA120-FITC binding to L. casei DG; Table 4. Repeatability of ELLSA detection of different quantities of RCA120-B binding to L. casei DG; Table 5. Reproducibility of BanLec-B binding to 21 different microorganisms. Experiments were done in three different laboratories, with preparing new plates each time; Fig. 1 Determining linearity of ELLSA method, by measuring binding between C.albicans and different quantity of BanLec-B; Fig. 2 Determining linearity of flow cytometry, by measuring binding between C.albicans and different quantity of BanLec-eGFP; Fig. 3 Determining linearity of ELLSA method, by measuring binding between L.casei DG and different quantity of RCA120; Fig. 4 Determining linearity of flow cytometry, by measuring binding between L.casei DG and different quantity of RCA120.
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- 2022
40. The intriguing role of anti-commensal bacteria antibodies in sera of colitic rats
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Kovačević Jovanović, Vesna, Ćuruvija, Ivana, Stanojević, Stanislava, Blagojević, Veljko, Kovačević Jovanović, Vesna, Ćuruvija, Ivana, Stanojević, Stanislava, and Blagojević, Veljko
- Abstract
BACKGROUND A variety of commensal bacterial taxa elicit serum IgA responses resulting in protection against polymicrobial sepsis, whereas anti-commensal IgG may have deleterious role in gastrointestinal and systemic inflammation. OBJECTIVES The aim was to determine the systemic levels of specific antibodies directed to autologous E.coli in rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains during colitis. METHODS Rats were intrarectally injected with ethanol or trinitrobenzenesulfonic acid (TNBS, 10 or 40mg/kg) whereas controls received saline in the same manner. Sera were tested for the level of anti-E.coli antibodies of IgG1, IgG2a, IgG2b and IgA classes by ELISA. RESULTS Both rat strains developed colitis, but the degree of colon necrosis and hyperemia were slightly greater in DA than in AO rats and the survival rate was significantly lower in DA relative to AO rats. Among saline-treated controls, the levels of IgG1, IgG2a, and IgG2b anti-E.coli antibodies were comparable between rat strains, whereas the amounts of IgA were significantly higher in DA compared to AO rats. Development of colitis significantly increased the amount of anti-E.coli antibodies of IgA and IgG2a classes in sera of AO rats, and those of IgG2a and IgG2b classes in sera of DA rats. Hence, mostly beneficial role of IgA and probably deleterious role of IgG2b antibodies directed to commensal E.coli during colitis may be suggested, whereas the role of anti-E.coli antibodies of IgG2a classes remains intriguing.
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- 2022
41. BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?
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Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, and Stanojević, Stanislava
- Abstract
BCG vaccination induces a memory-like response in innate immune cells known as trained immunity. In this study, we investigated the modification of innate immune cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were examined for 7 days after TG injection by flow cytometry, and for NO production and peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage cells as following: increased in vitro LPS and BCG stimulated NO production from total cells compared to adherent cells (day 1); increased cell number (days 3 and 5); increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On day 7, cells from both experimental groups showed no production of NO in response to in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the acute phase of sterile inflammation, which may lead to the later observed phenotypic and functional changes that could be seen as accelerated resolution of inflammation and possibly point to trained immune response.
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- 2022
42. STRATEŠKO PROMIŠLJANJE VOJNE NEUTRALNOSTI SRBIJE: MOGUĆNOSTI, IZAZOVI I RIZICI
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Blagojević, Veljko, primary
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- 2022
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43. Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat
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Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, Vujić, Vesna, Ćuruvija, Ivana, Blagojević, Veljko, and Leposavić, Gordana
- Published
- 2015
- Full Text
- View/download PDF
44. Sex differences in the effects of early-life probiotic treatment on TNBS-induced colitis in rats
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Blagojević, Veljko, Vujić, Vesna, Ćuruvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Stanojević, Stanislava, Blagojević, Veljko, Vujić, Vesna, Ćuruvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, and Stanojević, Stanislava
- Published
- 2021
45. Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment
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Nacka-Aleksić, Mirjana, Stojić-Vukanić, Zorica, Pilipović, Ivan, Blagojević, Veljko, Kotur-Stevuljević, Jelena, Leposavić, Gordana, Nacka-Aleksić, Mirjana, Stojić-Vukanić, Zorica, Pilipović, Ivan, Blagojević, Veljko, Kotur-Stevuljević, Jelena, and Leposavić, Gordana
- Abstract
The study examined the influence of sex on the alterations occurring with ageing in rat lymph node (LN) T cell compartment. In female and male rats the decrease in LN T cell counts was followed by a shift in CD4+/CD8+ T cell ratio towards CD8+ T cells, which was more prominent in males than in females. With ageing, in both major LN T cell subpopulations naive (recent thymic emigrants and mature naive cells) to memory/activated T cell ratio shifted to the side of memory/activated cells in female, and particularly in male rats. The frequency of regulatory CD25+Foxp3+ cells increased among LN CD4+/CD8+ T cells with ageing, reflecting, at least partly, an enhanced conversion of effector T cells into regulatory cells. This was also more prominent in male rats. The more prounounced increase in LN oxidative damage and the expression levels of proinflammatory cytokines in male rats with ageing, most likely contributed to the greater frequency of proinflammatory, replicatively senescent CD28- cells expressing CD11b (innate cell marker), among T cells of old male rats compared with age matched females. The increase in LN oxidation/proinflammatory state with ageing was also consistent with the accumulation of exhausted PD-1(high) cells among T lymphocytes, particularly prominent among CD8+ T cells from male rats. Finally, by calculating a summary score for the key ageing-relevant parameters (an ageing index), a faster development of the deleterious changes in the T cell compartment occurring with ageing was confirmed in male rat LNs. Additionally, the study pointed to indices of LN T cell compartment ageing which correlate with those in peripheral blood.
- Published
- 2020
46. Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment
- Author
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Nacka-Aleksić, Mirjana, primary, Stojić-Vukanić, Zorica, additional, Pilipović, Ivan, additional, Blagojević, Veljko, additional, Kotur-Stevuljević, Jelena, additional, and Leposavić, Gordana, additional
- Published
- 2020
- Full Text
- View/download PDF
47. Sudbina jedne globalne geopolitičke procene - Stepić Milomir: Buduća blokovska struktura sveta
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Blagojević, Veljko, primary
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- 2020
- Full Text
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48. Noradrenaline through beta-adrenoceptor contributes to sexual dimorphism in primary CD4+T-cell response in DA rat EAE model?
- Author
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Vujnović, Ivana, Pilipović, Ivan, Jasnić, Nebojša, Petrović, Raisa, Blagojević, Veljko, Arsenović-Ranin, Nevena, Stojić-Vukanić, Zorica, Đorđević, Jelena, Leposavić, Gordana, Vujnović, Ivana, Pilipović, Ivan, Jasnić, Nebojša, Petrović, Raisa, Blagojević, Veljko, Arsenović-Ranin, Nevena, Stojić-Vukanić, Zorica, Đorđević, Jelena, and Leposavić, Gordana
- Abstract
Males exhibit stronger sympathetic nervous system (SNS) activity, but weaker primary CD4 + T-cell (auto) immune responses. To test the role of catecholamines, major end-point SNS mediators, in this dimorphism, influence of propranolol (beta-adrenoceptor blocker) on mitogen/neuroantigen-stimulated CD4 + T cells from female and male EAE rat draining lymph node (dLN) cell cultures was examined. Male rat dLNs exhibited higher noradrenaline concentration and frequency of beta(2)-adrenoceptor-expressing CD4 + T lymphocytes and antigen presenting cells. Propranolol, irrespective of exogenous noradrenaline presence, more prominently augmented IL-2 production and proliferation of CD4 + lymphocytes in male than female rat dLN cell cultures. In neuroantigen-stimulated dLN cells of both sexes propranolol increased IL-1 beta and IL-23/p19 expression and IL-17 + CD4 + cell frequency, but enhanced IL-17 production only in male rat CD4 + lymphocytes, thereby abrogating sexual dimorphism in IL-17 concentration observed in propranolol-free cultures. Thus, beta-adrenoceptor-mediated signalling may contribute to sex bias in rat IL-17-producing cell secretory capacity.
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- 2019
49. Potential impact of early-life probiotic supplementation on peritoneal macrophage function
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Blagojević, Veljko, Petrović, Raisa, Ćuruvija, Ivana, Prijić, Ivana, Vujić, Vesna, Stanojević, Stanislava, Blagojević, Veljko, Petrović, Raisa, Ćuruvija, Ivana, Prijić, Ivana, Vujić, Vesna, and Stanojević, Stanislava
- Abstract
Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.
- Published
- 2019
50. The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats
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Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, and Vujić, Vesna
- Abstract
The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute
- Published
- 2018
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