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2. Faire gras à Molène: dairy products and ruminant fats detected by lipid and isotopic analysis of pottery dating to the Final Neolithic-Early Bronze Age from the island site of Beg ar Loued (Molène, western Brittany, France)

Author

PRÉVOST, Camielsa, primary, SURYANARAYAN, Akshyeta, additional, PAILLER, Yvan, additional, NICOLAS, Clément, additional, BLASCO, Thierry, additional, MAZUY, Arnaud, additional, HANOT, Pauline, additional, DRÉANO, Yvon, additional, DUPONT, Catherine, additional, and REGERT, Martine, additional

Published
2024
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7. Defining pottery use and exploitation of natural products at Clairvaux XIV during the Middle Neolithic

Author

Drieu, Léa, Mirabaud, Sigrid, Roffet-Salque, Mélanie, Blasco, Thierry, Pétrequin, Pierre, Pétrequin, Anne-Marie, Evershed, Richard P, and Regert, Martine

Subjects
lipids, vessel use, organic residues, carbon isotopes, cardiovascular system, dairying, Lake dwelling site, Middle Neolithic
Abstract

Investigating pottery function requires optimal preservation of both ceramic vessels and organic residues related to vessel use. If these criteria are met then opportunities emerge for combining macroscopic observations, typological classification, and capacity measurements and molecular and isotopic proxies (GC, GC-MS, and GC-C-IRMS) to explore vessel use and resource exploitation. The exceptional ceramic assemblage of hundreds of vessels excavated from the Middle Neolithic site of Clairvaux XIV (Jura, France, IVth millennium B.C.) offered such an opportunity, with a wide diversity of vessel shapes and extraordinary preservation of lipids. Interrogating the lipid residues together with typological analyses revealed a complex system of ceramic vessel use. Despite the scarcity of aquatic products, a wide diversity of natural substances has been detected: ruminant adipose fats, dairy products, plants substances, and beeswax. These commodities were processed in specific vessels with three main different functions: cooking vessels of various volumes, serving dishes for animal products, and dedicated vessels for dairy product processing and consumption.

Published
2022

8. Os, graines, charbons, fumier et isotopes : des archives biologiques pour comprendre le système agropastoral néolithique en moyenne montagne

Author

Gourichon, Lionel, Vuillien, Manon, Delhon, Claire, Herrscher, Estelle, Blasco, Thierry, Montanes, Maryline, Martin, L, Lepère, Cédric, Battentier, Janet, André, Guy, Carré, Alain, Mazuy, Arnaud, Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Archéozoologie, archéobotanique : sociétés, pratiques et environnements (AASPE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches Pétrographiques et Géochimiques (CRPG), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Universiteit Utrecht, EVEHA (Etudes et valorisations archeologiques), Centre Camille Jullian - Histoire et archéologie de la Méditerranée et de l'Afrique du Nord de la protohistoire à la fin de l'Antiquité (CCJ), and Aix Marseille Université (AMU)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory
Abstract

International audience

Published
2022

10. Nourrir la terre, nourrir le troupeau : approches bioarchéologiques et isotopiques des systèmes agropastoraux au Néolithique

Author

Martin, L, Delhon, Claire, Herrscher, Estelle, Blasco, Thierry, Montanes, Maryline, Vuillien, Manon, Gourichon, Lionel, Lepère, Cédric, Battentier, Janet, André, Guy, Carré, Alain, Mazuy, Arnaud, Centre de Recherches Pétrographiques et Géochimiques (CRPG), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Universiteit Utrecht, Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Archéozoologie, archéobotanique : sociétés, pratiques et environnements (AASPE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), EVEHA (Etudes et valorisations archeologiques), Centre Camille Jullian - Histoire et archéologie de la Méditerranée et de l'Afrique du Nord de la protohistoire à la fin de l'Antiquité (CCJ), Aix Marseille Université (AMU)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), and GMPCA

Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory
Abstract

International audience

Published
2022

11. Feeding the earth, feeding the herd: bioarchaeological and isotopic approaches to Neolithic agropastoral systems

Author

Martin, Lucie, Delhon, Claire, Herrscher, Estelle, Blasco, Thierry, Montanes, Maryline, Vuillien, Manon, Lepère, Cédric, Battentier, Janet, André, Guy, Carré, Alain, Mazuy, Arnaud, Environnements, Dynamiques et Territoires de la Montagne (EDYTEM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), EVEHA (Etudes et valorisations archeologiques), Centre Camille Jullian - Histoire et archéologie de la Méditerranée et de l'Afrique du Nord de la protohistoire à la fin de l'Antiquité (CCJ), Aix Marseille Université (AMU)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Environnements, Dynamiques et Territoires de Montagne (EDYTEM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), CEPAM, and Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])

Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

12. Identifying short-term climatic changes through isotopic charcoal analyses early Holocene warming and the '9.3 ky' event at the Mesolithic site of La Baume de Monthiver (Var, France)

Author

Audiard, Benjamin, Ricci, Giulia, Porraz, Guillaume, Purdue, Louise, Tomasso, Antonin, Mologni, Carlo, Blasco, Thierry, Battipaglia, Giovanna, Thery-Parisot, Isabelle, Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Centre National de la Recherche Scientifique (CNRS), Service Départemental d’Archéologie du Var, Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and University of the Study of Campania Luigi Vanvitelli

Subjects
isotopie (δ 13 C), Prealps, Paleoclimate, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, 3 ka », paléoclimat, Anthracologie, événement « 9, Isotopy (δ 13 C), "9.3 ky" event, Préalpes Anthracology
Abstract

Methodological approaches that allow the characterization of environmental and climatic changes on complementary tem-poral and geographical scales are essential in the study of the response of ancient societies to changes in their environment.Here, through the combining taxonomic and isotopic (δ13 C) analysis of archaeological charcoal from the Mesolithic site of La Baume de Monthiver (Var, France) with sub-stratigraphic sampling, we highlight the potential of this method to provide a highly sensitive proxy for long-term environmental trends as well as more rapid climatic episodes. Thus, we observe at the site: (i) the general taxonomic composition of the tree cover for the study phase, (ii) a high-resolution isotopic signal highlighting short-term climatic evolution, interpreted as early Holocene warming and/or the “9.3 ka” dry climate event.; Les approches permettant de caractériser les évolutions environ-nementales et climatiques à différentes échelles temporelles/géographiques sont essentielles dans l’étude de la réponse des sociétés anciennes faces aux changements de leur milieu. Ici, à travers l’application combinée de l’analyse taxinomique et isotopique (δ13 C) des charbons archéologiques des charbons du site mésolithique de La Baume de Monthiver (Var, France) avec un échantillonnage sous-stratigraphique, nous soulignons le potentiel de cette méthode pour fournir un proxy de haute sensibilité renseignant les tendances environnementales à long terme comme les épisodes climatiques plus rapides.On observe ainsi sur le site : (i) la composition taxinomique du cou-vert arborée de la phase d’étude, (ii) une évolution climatique à court terme, interprétée comme le réchauffement du début de l’Holocène et/ou l’événement climatique sec de « 9,3 ka »

Published
2021

15. Impact of dust addition on the metabolism of Mediterranean plankton communities and carbon export under present and future conditions of pH and temperature

Author

Gazeau, Frédéric, primary, Van Wambeke, France, additional, Marañón, Emilio, additional, Pérez-Lorenzo, Maria, additional, Alliouane, Samir, additional, Stolpe, Christian, additional, Blasco, Thierry, additional, Leblond, Nathalie, additional, Zäncker, Birthe, additional, Engel, Anja, additional, Marie, Barbara, additional, Dinasquet, Julie, additional, and Guieu, Cécile, additional

Published
2021
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17. Impact of dust addition on Mediterranean plankton communities under present and future conditions of pH and temperature: an experimental overview

Author

Gazeau, Frédéric, Van Wambeke, France, Maranon, Emilio, Perez-lorenzo, Maria, Alliouane, Samir, Stolpe, Christian, Blasco, Thierry, Leblond, Nathalie, Zancker, Birthe, Engel, Anja, Marie, Barbara, Dinasquet, Julie, and Guieu, Cécile

Subjects
Ocean warming, Carbon export, Ocean acidification, Mediterranean Sea, Plankton community metabolism, Atmospheric deposition
Abstract

Although atmospheric dust fluxes from arid as well as human-impacted areas represent a significant source of nutrients to surface waters of the Mediterranean Sea, studies focusing on the evolution of the metabolic balance of the plankton community following a dust deposition event are scarce and none were conducted in the context of projected future levels of temperature and pH. Moreover, most of the experiments took place in coastal areas. In the framework of the PEACETIME project, three dust-addition perturbation experiments were conducted in 300-L tanks filled with surface seawater collected in the Tyrrhenian Sea (TYR), Ionian Sea (ION) and in the Algerian basin (FAST) onboard the R/V “Pourquoi Pas?” in late spring 2017. For each experiment, six tanks were used to follow the evolution of chemical and biological stocks, biological activity and particle export. The impacts of a dust deposition event simulated at their surface were followed under present environmental conditions and under a realistic climate change scenario for 2100 (ca. +3 °C and −0.3 pH units). The tested waters were all typical of stratified oligotrophic conditions encountered in the open Mediterranean Sea at this period of the year, with low rates of primary production and a metabolic balance towards net heterotrophy. The release of nutrients after dust seeding had very contrasting impacts on the metabolism of the communities, depending on the station investigated. At TYR, the release of new nutrients was followed by a negative impact on both particulate and dissolved 14C-based production rates, while heterotrophic bacterial production strongly increased, driving the community to an even more heterotrophic state. At ION and FAST, the efficiency of organic matter export due to mineral/organic aggregation processes was lower than at TYR likely related to a lower quantity/age of dissolved organic matter present at the time of the seeding. At these stations, both the autotrophic and heterotrophic community benefited from dust addition, with a stronger relative increase in autotrophic processes observed at FAST. Our study showed that the potential positive impact of dust deposition on primary production depends on the initial composition and metabolic state of the investigated community. This potential is constrained by the quantity of nutrients added in order to sustain both the fast response of heterotrophic prokaryotes and the delayed one of primary producers. Finally, under future environmental conditions, heterotrophic metabolism was overall more impacted than primary production, with the consequence that all integrated net community production rates decreased with no detectable impact on carbon export, therefore reducing the capacity of surface waters to sequester anthropogenic CO2.

Published
2021

18. Impact of dust addition on the metabolism of Mediterranean plankton communities and carbon export under present and future conditions of pH and temperature

Author

Gazeau, Frédéric, Van Wambeke, France, Marañón, Emilio, Pérez-Lorenzo, Maria, Alliouane, Samir, Stolpe, Christian, Blasco, Thierry, Leblond, Nathalie, Zäncker, Birthe, Engel, Anja, Marie, Barbara, Dinasquet, Julie, Guieu, Cécile, Gazeau, Frédéric, Van Wambeke, France, Marañón, Emilio, Pérez-Lorenzo, Maria, Alliouane, Samir, Stolpe, Christian, Blasco, Thierry, Leblond, Nathalie, Zäncker, Birthe, Engel, Anja, Marie, Barbara, Dinasquet, Julie, and Guieu, Cécile

Abstract

Although atmospheric dust fluxes from arid as well as human-impacted areas represent a significant source of nutrients to surface waters of the Mediterranean Sea, studies focusing on the evolution of the metabolic balance of the plankton community following a dust deposition event are scarce, and none were conducted in the context of projected future levels of temperature and pH. Moreover, most of the experiments took place in coastal areas. In the framework of the PEACETIME project, three dust-addition perturbation experiments were conducted in 300 L tanks filled with surface seawater collected in the Tyrrhenian Sea (TYR), Ionian Sea (ION) and Algerian basin (FAST) on board the R/V Pourquoi Pas? in late spring 2017. For each experiment, six tanks were used to follow the evolution of chemical and biological stocks, biological activity and particle export. The impacts of a dust deposition event simulated at their surface were followed under present environmental conditions and under a realistic climate change scenario for 2100 (ca. +3 ∘C and −0.3 pH units). The tested waters were all typical of stratified oligotrophic conditions encountered in the open Mediterranean Sea at this period of the year, with low rates of primary production and a metabolic balance towards net heterotrophy. The release of nutrients after dust seeding had very contrasting impacts on the metabolism of the communities, depending on the station investigated. At TYR, the release of new nutrients was followed by a negative impact on both particulate and dissolved 14C-based production rates, while heterotrophic bacterial production strongly increased, driving the community to an even more heterotrophic state. At ION and FAST, the efficiency of organic matter export due to mineral/organic aggregation processes was lower than at TYR and likely related to a lower quantity/age of dissolved organic matter present at the time of the seeding and a smaller production of DOM following dust addition. This wa

Published
2021
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19. Independence of a Marine Unicellular Diazotroph to the Presence of NO3−

Author

Rabouille, Sophie, Randall, Benjamin, Talec, Amélie, Raimbault, Patrick, Blasco, Thierry, Latifi, Amel, Oschlies, Andreas, Rabouille, Sophie, Randall, Benjamin, Talec, Amélie, Raimbault, Patrick, Blasco, Thierry, Latifi, Amel, and Oschlies, Andreas

Abstract

Marine nitrogen (N2 ) fixation was historically considered to be absent or reduced in nitrate (NO3− ) rich environments. This is commonly attributed to the lower energetic cost of NO3− uptake compared to diazotrophy in oxic environments. This paradigm often contributes to making inferences about diazotroph distribution and activity in the ocean, and is also often used in biogeochemical ocean models. To assess the general validity of this paradigm beyond the traditionally used model organism Trichodesmium spp., we grew cultures of the unicellular cyanobacterium Crocosphaera watsonii WH8501 long term in medium containing replete concentrations of NO3− . NO3− uptake was measured in comparison to N2 fixation to assess the cultures’ nitrogen source preferences. We further measured culture growth rate, cell stoichiometry, and carbon fixation rate to determine if the presence of NO3 − had any effect on cell metabolism. We found that uptake of NO3− by this strain of Crocosphaera was minimal in comparison to other N sources (~2–4% of total uptake). Furthermore, availability of NO3 − did not statistically alter N2 fixation rate nor any aspect of cell physiology or metabolism measured (cellular growth rate, cell stoichiometry, cell size, nitrogen fixation rate, nitrogenase activity) in comparison to a NO3− free control culture. These results demonstrate the capability of a marine diazotroph to fix nitrogen and grow independently of NO3− . This lack of sensitivity of diazotrophy to NO3− suggests that assumptions often made about, and model formulations of, N2 fixation should be reconsidered.

Published
2021
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20. In-source fragmentation of very labile peptides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Author

Sachon, Emmanuelle, Clodic, Gilles, Blasco, Thierry, Jacquot, Yves, and Bolbach, Gerard

Subjects
Time-of-flight mass spectrometry -- Methods, Peptides -- Properties, Desorption -- Observations, Chemistry, Analytic -- Research, Chemistry
Abstract

Synthetic acidic praline-rich peptides devoid of basic chemical groups were studied by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). Their ion mass spectra recorded in reflector positive ion mode have shown unusual features, i.e., absence or very weak presence of protonated peptide together with a major peak associated with fragmentation at a site that corresponds to the amide bond N-terminal to the first proline of the XPP motif. In contrast, arginine-containing analogues were stable in MALDI-TOF, whereas peptides sharing a free N-terminal amino group were moderately subject to the same fragmentation. Effects of extraction delay time suggest that this process takes place very early (nanoseconds) at the beginning of the plume expansion. The effect of the nature of the matrix on the survival yield indicates a better correlation with the initial axial velocity than with the matrix proton affinity. All the data show some strong differences with the classical in-source decay (ISD). Our results suggest the role of the available protons in the close neighborhood of the peptide during the crystallization process and the prompt fragmentation induced by collisions in the first step of ablation. Undoubtedly, our study highlights that the MALDI-TOF analysis of peptides containing praline and no basic group should be carried out with extreme caution. 10.1021/ac901449d

Published
2009

21. Supplementary material to "Impact of dust addition on the metabolism of Mediterranean plankton communities and carbon export under present and future conditions of pH and temperature"

Author

Gazeau, Frédéric, primary, Van Wambeke, France, additional, Marañón, Emilio, additional, Pérez-Lorenzo, Maria, additional, Alliouane, Samir, additional, Stolpe, Christian, additional, Blasco, Thierry, additional, Leblond, Nathalie, additional, Zäncker, Birthe, additional, Engel, Anja, additional, Marie, Barbara, additional, Dinasquet, Julie, additional, and Guieu, Cécile, additional

Published
2021
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23. The use of stable isotopes to measure the ingestion rate of 1 potentially toxic benthic dinoflagellates by harpacticoid copepods

Author

Boisnoir, Aurélie, Pavaux, Anne-Sophie, Schizas, Nikolaos, Marro, Sophie, Blasco, Thierry, Lemée, Rodolphe, Pascal, Pierre-Yves, Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Puerto Rico at Mayagüez (UPR-M), Observatoire océanologique de Villefranche-sur-mer (OOVM), and Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, grazing rate, Ostreopsis cf ovata, Amphidinium cf carterae, fungi, meiofauna, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, 16 food web, isotope, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

International audience; Phycotoxins synthesized by benthic dinoflagellates are known to bioaccumulate in macrofauna and hence represent a risk for human health. However, the presence of toxins synthesized by benthic dinoflagellates in smaller marine organisms than macrofauna has not been considered despite the fact that such small organisms have an important ecological role in the benthic food web. This present study quantified, for the first time, the trophic relationship between benthic dinoflagellates and meiofauna by using stable isotope enriched dinoflagellates during ingestion experiments. Results showed that harpacticoid copepods were not able to discriminate, during ingestion, between the potentially toxic cells of Ostreopsis cf. ovata and the non-toxic cells of Amphidinium cf. carterae, even when another food resource, such as diatoms (e.g. Odontella sp.), was provided (Kruskal Wallis test, p > 0.05).

Published
2020

26. New climatic approaches of the middle Paleolithic sequences: comibined methodology of the taxonomic and isotopic charcoal analyses on two Neanderthals settlements: 'Les Canalettes ' (Aveyron, France) and 'la Combette' (Vaucluse, France)

Author

AUDIARD, Benjamin, Théry-Parisot, Isabelle, Meignen, Liliane, Texier, Pierre-Jean, Blasco, Thierry, Battipaglia, Giovanna, Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), and University of Naples Federico II

Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, ComputingMilieux_MISCELLANEOUS, [SHS]Humanities and Social Sciences
Abstract

International audience

Published
2019

28. Potentiel de l’anthracologie couplée à l’utilisation du signal isotopique (δ13C) des charbons de bois archéologiques pour l’étude des paléoclimats : approche méthodologiques et applications pour l’étude des gisements pléistocène

Author

Benjamin, AUDIARD, Blasco, Thierry, Brossier, Benoît, TEXIER, Pierre Jean, Battipaglia, Giovanna, THERY−PARISOT, Isabelle, Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), and University of Naples Federico II

Subjects
bois, isotopique, méthodologiques, applications, pour, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, l’étude, couplée, l’utilisation, paléoclimats, gisements, pléistocène, δ13C, Potentiel, charbons, archéologiques, signal, l’anthracologie, approche, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

29. Nouvelle méthode d'étude des paléo-environnements : création d'un référentiel actuel du signal isotopique présent dans une espèce clé : le pin sylvestre; protocole et premier résultats

Author

AUDIARD, Benjamin, Blasco, Thierry, Brossier Benoit, Pierre-Jean, Battipaglia, Giovanna, Théry-Parisot, Isabelle and, Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), and University of Naples Federico II

Subjects
sylvestre, isotopique, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, d'un, d'étude, espèce, dans, présent, résultats, méthode, référentiel, premier, actuel, création, paléo-environnements, signal, protocole, Nouvelle
Abstract

Nouvelle méthode d'étude des paléo-environnements : création d'un référentiel actuel du signal isotopique présent dans une espèce clé : le pin sylvestre; protocole et premier résultats

Published
2017

30. Etude par GC-c-IRMS de la métabolisation et bioaccumulation du bisphénol A et de sa toxicité dans l’ascidie Phallusia mammillata

Author

Gendre, Heloise, Gomes, I, Dumollard, Rémy, Migon, Christophe, Blasco, Thierry, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Observatoire océanologique de Villefranche-sur-mer (OOVM), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract

International audience

Published
2017

32. δ13C referential in three Pinusspecies for a first archaeological application to Paleolithic contexts: “Between intra- and inter-individual variation and carbonization effect”

Author

Audiard, Benjamin, Blasco, Thierry, Brossier, Benoit, Fiorentino, Girolamo, Battipaglia, Giovanna, and Théry-Parisot, Isabelle

Abstract

Tree-ring studies, particularly those focusing on the δ13C isotopic signal, have become a reliable method to reconstruct past climatic and environmental conditions. Unfortunately, wood remains are notably absent from the very ancient archaeological record, due to degradability of plant tissue. For archaeological studies, the analysis of isotopic signals from charcoal of Pinusspecies, which is more reliably preserved over time, and continuously represented among archaeological remains, would yield more robust findings. However, methodological confirmation of this approach is required prior to the archaeological implementation of this tool. Here, we present a wood-charcoal reference set, focused on the intra-genus Pinusspp. Δ13C data (Pinus halepensisL., Pinus sylvestrisMill., Pinus nigrasubsp. nigraArn.), which addresses both natural variability and the isotopic impact of the carbonization. Our results show the potential application of an average isotopic signal from different Pinusspp. either from wood and charcoal. Moreover, carbonization results offer a baseline from which to create a general correction of the δ13C shift introduced during pyrolysis temperature gradients. Lastly, a primary reference set was established in the context of climatic and environmental parameters for future archaeological analyses of charcoal remains.

Published
2018
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35. MALDI TOFTOF characterization of a light stabilizer polymer contaminant from polypropylene or polyethylene plastic test tubes

Author

Sachon, Emmanuelle, Matheron, Lucrèce, Clodic, Gilles, Blasco, Thierry, and Bolbach, Gérard

Abstract

Disposable plasticware such as plastic test tubes are routinely used in all proteomics laboratories. Additives in polymers are used to protect them against oxygen or ultraviolet UV light degradation. Hindered amine light stabilizers HALSs are of utmost importance in modern polyolefin polypropylene, polyethylene stabilization. In this article, we demonstrate that the manufacturing polymeric agent: polyNβhydroxyethyl2,2,6,6tetramethyl4hydroxypiperidinyl succinate, known as Tinuvin622 or Lowilite 62, from the HALS family, leaches from laboratory polypropylene or polyethylene plastic test tubes into the standard solvents for sample preparation. The analysis of these polluted samples by matrixassisted laser desorptionionisationtime of flight MALDITOF mass spectrometry, in the positive mode, shows highly contaminated mass spectra, due to the high sensitivity of this technique. These contaminants have mass range and mass defect similar to those of peptides arising from the digestion of a protein in a conventional proteomics study. Therefore, they can be really harmful for proteomics studies, leading to misattributions, preventing any protein identification. In this article, an MS and MSMS fingerprint of this pollutant is given and some pieces of advice to avoid it are proposed. Copyright © 2009 John Wiley & Sons, Ltd.

Published
2010
Full Text
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36. Assessment of Lipid Quality in Commercial Omega-3 Supplements Sold in the French Market

Author

Federica Pasini, Ana Maria Gómez-Caravaca, Thierry Blasco, Jelena Cvejić, Maria Fiorenza Caboni, Vito Verardo, Pasini, Federica, Gómez-Caravaca, Ana Maria, Blasco, Thierry, Cvejić, Jelena, Caboni, Maria Fiorenza, and Verardo, Vito

Subjects
Docosahexaenoic Acids, Schizochytrium, Tocopherols, Fish oil, Microalgae oil, Peroxide, Biochemistry, Triglyceride, Fish Oils, lipid oxidation, Fatty Acids, Omega-3, peroxide value, Molecular Biology, Triglycerides, Dietary Supplement, Docosahexaenoic Acid, microalgae oil, Tocopherol, Tocotrienols, Tocotrienol, Fatty Acids, Fish Oil, polyunsaturated fatty acid, Lipid oxidation, Peroxides, Eicosapentaenoic Acid, polyunsaturated fatty acids, fish oil, p-anisidine value, Totox, Peroxide value, Dietary Supplements, Polyunsaturated fatty acids, Fatty Acid
Abstract

Supplementation of omega-3 fatty acids is considered a valuable strategy to supply the low intake of these fatty acids. Thus, the safety of the supplements is an important milestone. Because of that, we analyzed 20 unflavored supplements sold in the French market for fatty acid and triglyceride composition, for EPA and DHA, and for tocol content, as well as for oxidative status. This study found that only 2.5% of the supplements did not meet their label claims for omega-3 content. TAG analysis showed high variability among the triglyceride distribution, and the same trend was also noticed for the tocol content; in fact, a high variability of the distribution of the six tocols (four tocopherols and two tocotrienols) was found among the samples. Of the tested products, all of them complied with peroxide value, p-anisidine value, and Totox values established by the Global Organization for EPA and DHA Omega-3s (GOED) and were not oxidized., Spanish Ministry of Science, Education and Universities/Spanish State Research Agency PCI2018-093178, European Commission

Published
2022
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37. Crossing taxonomic and isotopic approaches in charcoal analyses to reveal past climates. New perspectives in Paleobotany from the Paleolithic Neanderthal dwelling-site of La Combette (Vaucluse, France)

Author

Benjamin Audiard, Giovanna Battipaglia, Pierre-Jean Texier, Isabelle Théry-Parisot, Thierry Blasco, Carlo Mologni, Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Géoazur (GEOAZUR 7329), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de la Côte d'Azur, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), University of the Study of Campania Luigi Vanvitelli, Audiard, Benjamin, Thery-Parisot, Isabelle, Blasco, Thierry, Mologni, Carlo, Texier, Pierre-Jean, Battipaglia, Giovanna, Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), and CCSD, Accord Elsevier

Subjects
0106 biological sciences, 010506 paleontology, Neanderthal, Pleistocene, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, Context (language use), 010603 evolutionary biology, 01 natural sciences, Paleolithic, biology.animal, Middle Paleolithic, vaucluse, Paleobotany, Charcoal, climate, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, Isotope analysis, Anthracology, Palynology, [SHS.ARCHEO] Humanities and Social Sciences/Archaeology and Prehistory, biology, Paleontology, 15. Life on land, Ecology, Evolution, Behavior and Systematic, Geography, 13. Climate action, visual_art, visual_art.visual_art_medium, Physical geography, France, charcoal
Abstract

International audience; Robust methodology in charcoal analyses has strong potential in the reconstruction of past environments. However, charcoal assemblages from Pleistocene contexts, particularly those that formed under varying climatic conditions during glaciation and mostly composed of the genus Pinus, can neither express changes in forest density, nor can they reveal climatic conditions. Here, we explore the potential of a combined approach using taxonomic and δ13C isotopic study of archeological charcoal from middle Paleolithic sequence of La Combette. In order to investigate past climate and/or environmental conditions, we focus on charcoal of Pinus spp. from infra stratigraphic units at the site that showed a high potential for isotope analysis. Those isotopic results are consistent with other paleoenvironnemental data (anthracology, micromorphology, palynology, etc.) that are manifested at different time scales. In some cases, isotopic data provide, more accurate information than that derived from taxonomic identifications. This is due to the quicker physiological response of the plants compared to the long-term changes in forest biodiversity under climatic pressure. Subsequently, this first application of cross taxonomic and isotopic approach offers promising and original results and must be applied to different context (low to high taphonomic process). Lastly, the degree of asynchrony or synchrony between taxonomic and isotopic findings allows us to rethink Neanderthal fuel management and mobility patterns and to explain the correlation between Neanderthal occupations of the rock shelter in view of climatic conditions.

Published
2019

38. δ13C referential in three Pinus species for a first archeological application to Paleolithic contexts 'Between intra- and inter-individual variation and carbonization effect

Author

Girolamo Fiorentino, Isabelle Théry-Parisot, Benoît Brossier, Thierry Blasco, Benjamin Audiard, Giovanna Battipaglia, Laboratoire d'océanographie de Villefranche (LOV), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Archeobotanica e Paleoecologia, Università del Salento [Lecce], Department of Environmental, Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Naples Federico II, Culture et Environnements, Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Audiard, Benjamin, Blasco, Thierry, Brossier, Benoit, Fiorentino, Girolamo, Battipaglia, Giovanna, and Théry-Parisot, Isabelle

Subjects
0106 biological sciences, Archeology, Stable isotopes Carbonization Charcoal Pinus Palaeoclimatology Pleistocene, 010504 meteorology & atmospheric sciences, Context (archaeology), [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, Archaeological record, species, 01 natural sciences, contexts, intra, Paleolithic, Natural variability, Charcoal, inter-individual, Pinu, 0105 earth and related environmental sciences, δ13C, Carbonization, effect, referential, carbonization, “Between, 15. Life on land, Pinus, Stable isotope, Archaeology, Plant tissue, Pleistocene, %22">Pinus , archeological, Palaeoclimatology, three, visual_art, [SHS.ENVIR]Humanities and Social Sciences/Environmental studies, visual_art.visual_art_medium, Environmental science, first, variation, application, 010606 plant biology & botany
Abstract

Tree-ring studies, particularly those focusing on the delta C-13 isotopic signal, have become a reliable method to reconstruct past climatic and environmental conditions. Unfortunately, wood remains are notably absent from the very ancient archaeological record, due to degradability of plant tissue. For archaeological studies, the analysis of isotopic signals from charcoal of Pinus species, which is more reliably preserved over time, and continuously represented among archaeological remains, would yield more robust findings. However, methodological confirmation of this approach is required prior to the archaeological implementation of this tool. Here, we present a wood-charcoal reference set, focused on the intra-genus Pinus spp. Delta C-13 data (Pinus halepensis L., Pinus sylvestris Mill., Pinus nigra subsp. nigra Arn.), which addresses both natural variability and the isotopic impact of the carbonization. Our results show the potential application of an average isotopic signal from different Pinus spp. either from wood and charcoal. Moreover, carbonization results offer a baseline from which to create a general correction of the delta C-13 shift introduced during pyrolysis temperature gradients. Lastly, a primary reference set was established in the context of climatic and environmental parameters for future archaeological analyses of charcoal remains.

Published
2018

39. MALDI TOF-TOF characterization of a light stabilizer polymer contaminant from polypropylene or polyethylene plastic test tubes.

Author

Sachon E, Matheron L, Clodic G, Blasco T, and Bolbach G

Subjects
Amines chemistry, Disposable Equipment, Proteomics, Sensitivity and Specificity, Serum Albumin, Bovine, Polyesters chemistry, Polyethylene chemistry, Polypropylenes chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Abstract

Disposable plasticware such as plastic test tubes are routinely used in all proteomics laboratories. Additives in polymers are used to protect them against oxygen or ultraviolet (UV) light degradation. Hindered amine light stabilizers (HALSs) are of utmost importance in modern polyolefin (polypropylene, polyethylene) stabilization. In this article, we demonstrate that the manufacturing polymeric agent: poly-(N-beta-hydroxyethyl-2,2,6,6-tetramethyl-4-hydroxy-piperidinyl succinate), known as Tinuvin-622 or Lowilite 62, from the HALS family, leaches from laboratory polypropylene or polyethylene plastic test tubes into the standard solvents for sample preparation. The analysis of these polluted samples by matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry, in the positive mode, shows highly contaminated mass spectra, due to the high sensitivity of this technique. These contaminants have mass range and mass defect similar to those of peptides arising from the digestion of a protein in a conventional proteomics study. Therefore, they can be really harmful for proteomics studies, leading to misattributions, preventing any protein identification. In this article, an MS and MS/MS fingerprint of this pollutant is given and some pieces of advice to avoid it are proposed., (Copyright 2009 John Wiley & Sons, Ltd.)

Published
2010
Full Text
View/download PDF

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