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1. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.

Author

Welsh, Joshua, Goberdhan, Deborah, ODriscoll, Lorraine, Buzas, Edit, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom, Erdbrügger, Uta, Falcon-Perez, Juan, Fu, Qing-Ling, Hill, Andrew, Lenassi, Metka, Lim, Sai, Mahoney, Mỹ, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther, Brucale, Marco, Burger, Dylan, Carney, Randy, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian, Haughey, Norman, Hendrix, An, Ivanov, Alexander, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole, Kuulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen, Lötvall, Jan, Maddox, Adam, Martens-Uzunova, Elena, Mizenko, Rachel, Newman, Lauren, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula, Saugstad, Julie, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn, Wauben, Marca, Wehman, Ann, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan, Théry, Clotilde, and Witwer, Kenneth

Subjects
MISEV, ectosomes, exosomes, extracellular particles, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardisation, Extracellular Vesicles, Exosomes, Biological Transport, Biomarkers, Phenotype
Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its Minimal Information for Studies of Extracellular Vesicles, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

3. Blending space and time to talk about cancer in extended reality

Author

Robb, Tamsin J., Liu, Yinan, Woodhouse, Braden, Windahl, Charlotta, Hurley, Daniel, McArthur, Grant, Fox, Stephen B., Brown, Lisa, Guilford, Parry, Minhinnick, Alice, Jackson, Christopher, Blenkiron, Cherie, Parker, Kate, Henare, Kimiora, McColl, Rose, Haux, Bianca, Young, Nick, Boyle, Veronica, Cameron, Laird, Deva, Sanjeev, Reeve, Jane, Print, Cristin G., Davis, Michael, Rieger, Uwe, and Lawrence, Ben

Published
2024
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7. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author

Théry, Clotilde, Witwer, Kenneth W, Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D, Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkin‐Smith, Georgia K, Ayre, D Craig, Bach, Jean‐Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N, Baxter, Amy A, Bebawy, Mary, Beckham, Carla, Zavec, Apolonija Bedina, Benmoussa, Abderrahim, Berardi, Anna C, Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis‐Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E, Bosch, Steffi, Boulanger, Chantal M, Breakefield, Xandra, Breglio, Andrew M, Brennan, Meadhbh Á, Brigstock, David R, Brisson, Alain, Broekman, Marike LD, Bromberg, Jacqueline F, Bryl‐Górecka, Paulina, Buch, Shilpa, Buck, Amy H, Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I, Byrd, James Bryan, Camussi, Giovanni, Carter, David RF, Caruso, Sarah, Chamley, Lawrence W, Chang, Yu‐Ting, Chen, Chihchen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R, Clayton, Aled, Clerici, Stefano P, Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J, Cordeiro‐da‐Silva, Anabela, Couch, Yvonne, Coumans, Frank AW, Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D'Souza‐Schorey, Crislyn, Das, Saumya, Chaudhuri, Amrita Datta, de Candia, Paola, De Santana, Eliezer F, De Wever, Olivier, del Portillo, Hernando A, Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C, Dolo, Vincenza, Rubio, Ana Paula Dominguez, Dominici, Massimo, Dourado, Mauricio R, Driedonks, Tom AP, Duarte, Filipe V, Duncan, Heather M, Eichenberger, Ramon M, Ekström, Karin, Andaloussi, Samir EL, Elie‐Caille, Celine, Erdbrügger, Uta, Falcón‐Pérez, Juan M, Fatima, Farah, Fish, Jason E, Flores‐Bellver, Miguel, Försönits, András, and Frelet‐Barrand, Annie

Subjects
Biochemistry and Cell Biology, Biological Sciences, extracellular vesicles, exosomes, ectosomes, microvesicles, minimal information requirements, guidelines, standardization, microparticles, rigor, reproducibility, Biochemistry and cell biology
Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Published
2018

8. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Author

Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W, Stoorvogel, Willem, Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Welsh, Joshua A, Goberdhan, Deborah C I, O'Driscoll, Lorraine, Buzas, Edit I, Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A P, Erdbrügger, Uta, Falcon-Perez, Juan M, Fu, Qing-Ling, Hill, Andrew F, Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G, Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C, Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M, Brucale, Marco, Burger, Dylan, Carney, Randy P, Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L, Haughey, Norman J, Hendrix, An, Ivanov, Alexander R, Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A, Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M, Lötvall, Jan, Maddox, Adam L, Martens-Uzunova, Elena S, Mizenko, Rachel R, Newman, Lauren A, Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S, Saugstad, Julie A, Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P, Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J C, Wauben, Marca H M, Wehman, Ann M, Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W, and Stoorvogel, Willem

Published
2024

9. Minimal information for studies of extracellular vesicles (MISEV2023) : from basic to advanced approaches

Author

Welsh, Joshua A., Goberdhan, Deborah C. I., O'Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing-Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Eichenberger, Ramon, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M., Lötvall, Jan, Maddox, Adam L., Martens-Uzunova, Elena S., Mizenko, Rachel R., Newman, Lauren A., Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S., Saugstad, Julie A., Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P., Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J. C., Wauben, Marca H. M., Wehman, Ann M., Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, Witwer, Kenneth W., Welsh, Joshua A., Goberdhan, Deborah C. I., O'Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing-Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Colombo, Federico, Crescitelli, Rossella, Eichenberger, Ramon, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Hendrix, An, Ivanov, Alexander R., Jovanovic-Talisman, Tijana, Kruh-Garcia, Nicole A., Ku'ulei-Lyn Faustino, Vroniqa, Kyburz, Diego, Lässer, Cecilia, Lennon, Kathleen M., Lötvall, Jan, Maddox, Adam L., Martens-Uzunova, Elena S., Mizenko, Rachel R., Newman, Lauren A., Ridolfi, Andrea, Rohde, Eva, Rojalin, Tatu, Rowland, Andrew, Saftics, Andras, Sandau, Ursula S., Saugstad, Julie A., Shekari, Faezeh, Swift, Simon, Ter-Ovanesyan, Dmitry, Tosar, Juan P., Useckaite, Zivile, Valle, Francesco, Varga, Zoltan, van der Pol, Edwin, van Herwijnen, Martijn J. C., Wauben, Marca H. M., Wehman, Ann M., Williams, Sarah, Zendrini, Andrea, Zimmerman, Alan J, Misev Consortium, Théry, Clotilde, and Witwer, Kenneth W.

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

10. Minimal information for studies of extracellular vesicles (MISEV2023):From basic to advanced approaches

Author

Welsh, Joshua A., Goberdhan, Deborah C.I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A.P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, M. ỹ.G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Benediktsdóttir, Berglind E., Gargiulo, Ernesto, Gori, Alessandro, Guo, Wei, Hufnagel, Antonia, Di Ianni, Emilio, Jacobsen, Soren, Li, Bo, Mellergaard, Maiken, Nejsum, Peter, Nolan, John P., Østergaard, Ole, Yarani, Reza, Yin, Hang, Welsh, Joshua A., Goberdhan, Deborah C.I., O’Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A.P., Erdbrügger, Uta, Falcon-Perez, Juan M., Fu, Qing Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, M. ỹ.G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, Ochiya, Takahiro, Sahoo, Susmita, Torrecilhas, Ana C., Zheng, Lei, Zijlstra, Andries, Abuelreich, Sarah, Bagabas, Reem, Bergese, Paolo, Bridges, Esther M., Brucale, Marco, Burger, Dylan, Carney, Randy P., Cocucci, Emanuele, Crescitelli, Rossella, Hanser, Edveena, Harris, Adrian L., Haughey, Norman J., Benediktsdóttir, Berglind E., Gargiulo, Ernesto, Gori, Alessandro, Guo, Wei, Hufnagel, Antonia, Di Ianni, Emilio, Jacobsen, Soren, Li, Bo, Mellergaard, Maiken, Nejsum, Peter, Nolan, John P., Østergaard, Ole, Yarani, Reza, and Yin, Hang

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly., Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Published
2024

16. The tumour-derived extracellular vesicle proteome varies by endometrial cancer histology and is confounded by an obesogenic environment

Author

Artuyants, Anastasiia, primary, Guo, George, additional, Flinterman, Marcella, additional, Middleditch, Martin, additional, Jacob, Bincy, additional, Lee, Kate, additional, Vella, Laura J., additional, Su, Huaqi, additional, Wilson, Michelle, additional, Eva, Lois, additional, Shelling, Andrew N., additional, and Blenkiron, Cherie, additional

Published
2023
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18. An integrated positional and functional approach for identifying ovarian cancer tumour suppressor genes on chromosome 11p

Author

Blenkiron, Cherie

Subjects
616.99
Abstract

Ovarian cancer represents the most lethal gynaecological malignancy in the UK. Numerous tumour suppressor genes (TSG) are postulated to be involved in the aetiology of epithelial ovarian cancer (EOC). Cytogenetic analyses of cancer cells by methods such as LOH and CGH, have identified regions of genomic aberration. Allele loss on chromosome 1 lp has frequently been implicated in ovarian cancers, suggesting the presence of TSGs in these regions. Ovarian cancer cell line OVCAR3 has lost a whole copy of chromosome 11. The remaining copy is fragmented, rearranged and duplicated. Transfer of normal chromosome 11 into OVCAR3 by Microcell Mediated Chromosome Transfer (MMCT) produced microcell hybrids that display suppression of growth and cellular migration in vitro and inhibition of tumour growth in vivo. Analysis of revertant clones was unable to further minimise regions harbouring candidate TSGs. Subsequently, mRNA populations from OHN, a clonal derivative of the OVCAR3 parent line, and from 110H2.1, a growth suppressed microcell hybrid, were used for expression difference analysis by Differential Display RT-PCR (DDRT-PCR), cDNA-Representational Difference Analysis (cDNA-RDA) and cDNA high density filter array (HDFA). In all, these techniques identified 159 up and 162 down regulated genes with respect to growth suppression. Quantitative real time RT-PCR was used to validate expression differences in 178 transcripts. We identified, in total, 12 validated upregulated products and 4 validated downregulated products. Of the 12 upregulated products associated with growth suppression, 4 were localised on chromosome 11, three at llpl5. These were cathepsin D (CTSD), proteasome subunit PSMD13, ribosomal subunit RPL27A on 11 p 15 and aB crystallin (CRYAB) on llq23. All were shown to have decreased expression in several ovarian cancer cell lines and primary tumours. Furthermore, a tight correlation was observed between the expression of PSMD13 and RPL27A in cell lines and primary ovarian tumours. Low expression of CTSD and CRYAB were associated with adverse survival in patients with ovarian cancers. The genes downregulated in association with growth suppression, and therefore of potentially oncogenic function, were RALDH2, IGFBP2 and 2 novel cDNAs. When examined on cell line and primary tumour panels, these genes did not however appear to demonstrate a global increase in expression over that of normal OSE. An extensive LOH analysis of 87 ovarian tumours and their matched normal samples was then performed. Thirty-nine microsatellite markers spanning 19.8Mb on 1 lp 15 were used in the most comprehensive analysis in ovarian cancer to date. Loss of the complete region was common (24%) and peaks of high LOH (> 35%) were seen for 12 markers. Six microsatellite markers showed an association with one or more clinicopathological variables (p < 0.01). Nine minimal regions of LOH were found. PSMD13 and CTSD were both found within these regions of LOH as characterised by the markers D11S2071 and D11S922. RPL27a resides on llpl5.4 near the marker D11S932 which was not located within a minimal region of loss but LOH of that marker was significantly associated with advanced FIGO stage (p=0.0001). This approach has demonstrated that the integration of functional and positional molecular genetic techniques can co-operate in the identification of candidate ovarian cancer TSGs.

Published
2003

19. Supplementary Figure S5 from Complex Patterns of Genomic Heterogeneity Identified in 42 Tumor Samples and ctDNA of a Pulmonary Atypical Carcinoid Patient

Author

Robb, Tamsin J., primary, Tsai, Peter, primary, Fitzgerald, Sandra, primary, Shields, Paula, primary, Houseman, Pascalene S., primary, Patel, Rachna, primary, Fan, Vicky, primary, Curran, Ben, primary, Tse, Rexson, primary, Ting, Jacklyn, primary, Kramer, Nicole, primary, Woodhouse, Braden J., primary, Coats, Esther, primary, Le Quesne Stabej, Polona, primary, Reeve, Jane, primary, Parker, Kate, primary, Lawrence, Ben, primary, Blenkiron, Cherie, primary, and Print, Cristin G., primary

Published
2023
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20. Data from Complex Patterns of Genomic Heterogeneity Identified in 42 Tumor Samples and ctDNA of a Pulmonary Atypical Carcinoid Patient

Author

Robb, Tamsin J., primary, Tsai, Peter, primary, Fitzgerald, Sandra, primary, Shields, Paula, primary, Houseman, Pascalene S., primary, Patel, Rachna, primary, Fan, Vicky, primary, Curran, Ben, primary, Tse, Rexson, primary, Ting, Jacklyn, primary, Kramer, Nicole, primary, Woodhouse, Braden J., primary, Coats, Esther, primary, Le Quesne Stabej, Polona, primary, Reeve, Jane, primary, Parker, Kate, primary, Lawrence, Ben, primary, Blenkiron, Cherie, primary, and Print, Cristin G., primary

Published
2023
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21. Supplementary Tables S1-S10 from Complex Patterns of Genomic Heterogeneity Identified in 42 Tumor Samples and ctDNA of a Pulmonary Atypical Carcinoid Patient

Author

Robb, Tamsin J., primary, Tsai, Peter, primary, Fitzgerald, Sandra, primary, Shields, Paula, primary, Houseman, Pascalene S., primary, Patel, Rachna, primary, Fan, Vicky, primary, Curran, Ben, primary, Tse, Rexson, primary, Ting, Jacklyn, primary, Kramer, Nicole, primary, Woodhouse, Braden J., primary, Coats, Esther, primary, Le Quesne Stabej, Polona, primary, Reeve, Jane, primary, Parker, Kate, primary, Lawrence, Ben, primary, Blenkiron, Cherie, primary, and Print, Cristin G., primary

Published
2023
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24. Complex Patterns of Genomic Heterogeneity Identified in 42 Tumor Samples and ctDNA of a Pulmonary Atypical Carcinoid Patient

Author

Robb, Tamsin J., primary, Tsai, Peter, additional, Fitzgerald, Sandra, additional, Shields, Paula, additional, Houseman, Pascalene S., additional, Patel, Rachna, additional, Fan, Vicky, additional, Curran, Ben, additional, Tse, Rexson, additional, Ting, Jacklyn, additional, Kramer, Nicole, additional, Woodhouse, Braden J., additional, Coats, Esther, additional, Le Quesne Stabej, Polona, additional, Reeve, Jane, additional, Parker, Kate, additional, Lawrence, Ben, additional, Blenkiron, Cherie, additional, and Print, Cristin G., additional

Published
2023
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27. Dynamic ctDNA mutational complexity in melanoma patients receiving immunotherapy

Author

Fitzgerald, Sandra, primary, Blenkiron, Cherie, additional, Stephens, Rosalie, additional, Mathy, Jon, additional, Somers-Edgar, Tiffany, additional, Rolfe, Gill, additional, Martin, Richard, additional, Jackson, Christopher, additional, Eccles, Michael, additional, Robb, Tamsin, additional, Rodger, Euan, additional, Lawrence, Ben, additional, Guilford, Parry, additional, Lasham, Annette, additional, and Print, Cristin, additional

Published
2022
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30. Recurrent loss of heterozygosity correlates with clinical outcome in pancreatic neuroendocrine cancer

Author

Lawrence, Ben, Blenkiron, Cherie, Parker, Kate, Tsai, Peter, Fitzgerald, Sandra, Shields, Paula, Robb, Tamsin, Yeong, Mee Ling, Kramer, Nicole, James, Sarah, Black, Mik, Fan, Vicky, Poonawala, Nooriyah, Yap, Patrick, Coats, Esther, Woodhouse, Braden, Ramsaroop, Reena, Yozu, Masato, Robinson, Bridget, Henare, Kimiora, Koea, Jonathan, Johnston, Peter, Carroll, Richard, Connor, Saxon, Morrin, Helen, Elston, Marianne, Jackson, Christopher, Reid, Papaarangi, Windsor, John, MacCormick, Andrew, Babor, Richard, Bartlett, Adam, Damianovich, Dragan, Knowlton, Nicholas, Grimmond, Sean, Findlay, Michael, and Print, Cristin

Published
2018
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33. A Novel Electrochemically Switchable Conductive Polymer Interface for Controlled Capture and Release of Chemical and Biological Entities

Author

Akbarinejad, Alireza, primary, Hisey, Colin Lee, additional, Martinez‐Calderón, Miguel, additional, Low, Jeffery, additional, Bryant, Devon T., additional, Zhu, Bicheng, additional, Brewster, Diane, additional, Chan, Eddie Wai Chi, additional, Ashraf, Jesna, additional, Wan, Ziyao, additional, Artuyants, Anastasiia, additional, Blenkiron, Cherie, additional, Chamley, Larry, additional, Barker, David, additional, Williams, David E., additional, Evans, Clive W., additional, Pilkington, Lisa I., additional, and Travas‐Sejdic, Jadranka, additional

Published
2022
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34. Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

Author

Witwer, Kenneth W, primary, Goberdhan, Deborah CI, additional, O'Driscoll, Lorraine, additional, Théry, Clotilde, additional, Welsh, Joshua A, additional, Blenkiron, Cherie, additional, Buzás, Edit I, additional, Di Vizio, Dolores, additional, Erdbrügger, Uta, additional, Falcón‐Pérez, Juan M, additional, Fu, Qing‐Ling, additional, Hill, Andrew F, additional, Lenassi, Metka, additional, Lötvall, Jan, additional, Nieuwland, Rienk, additional, Ochiya, Takahiro, additional, Rome, Sophie, additional, Sahoo, Susmita, additional, and Zheng, Lei, additional

Published
2021
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41. The Extracellular Matrix Protein TGFBI Induces Microtubule Stabilization and Sensitizes Ovarian Cancers to Paclitaxel

Author

Ahmed, Ahmed Ashour, Mills, Anthony D., Ibrahim, Ashraf E.K., Temple, Jillian, Blenkiron, Cherie, Vias, Maria, Massie, Charlie E., Iyer, N. Gopalakrishna, McGeoch, Adam, Crawford, Robin, Nicke, Barbara, Downward, Julian, Swanton, Charles, Bell, Stephen D., Earl, Helena M., Laskey, Ronald A., Caldas, Carlos, and Brenton, James D.

Published
2007
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42. High-resolution magic angle spinning lH NMR spectroscopy and reverse transcription-PCR analysis of apoptosis in a rat glioma

Author

Griffin, Julian L., Blenkiron, Cherie, Valonen, Piia K., Caldas, Carlos, and Kauppinen, Risto A.

Subjects
Apoptosis -- Research, Nuclear magnetic resonance spectroscopy -- Analysis, Gliomas -- Diagnosis, Polymerase chain reaction -- Analysis, Chemistry
Abstract

The functional genomic approaches of transcriptomics, proteomics and metabolomics aim to measure the mRNA, protein or metabolite complement of a cell, tissue or organism. In this study we have investigated the compatibility of transcriptional analysis, using Reverse Transcription (RT)-PCR, and metabolite analysis, by high-resolution magic angle spinning (HRMAS) [sup.1]H NMR spectroscopy, in BT4C rat glioma following the induction of programmed cell death. The metabolite and transcriptional changes that accompanied apoptosis were examined at 0, 4 and 8 days of ganciclovir/thymidine kinase gene therapy. Despite the high spinning speeds employed during HRMAS [sup.1]H NMR spectroscopy of one-half of the tumor samples, RT-PCR analysis of the pro-apoptotic transcripts Bcl-2, BAK-1, caspase-9 and FAS was possible, producing similar results to those detected in the unspun half of the tumors. Furthermore, the expression of FAS was inversely correlated with some of the key metabolic changes across the time period examined including the increases CH = CH and CH = CHC[H.sub.2] lipid resonances which accompany apoptosis. This study demonstrates how combined transcriptomic and metabolomic studies of tumors can be used to understand the molecular events that accompany well documented metabolic perturbations during cell death processes.

Published
2006

47. Novel Electrochemically Switchable, Flexible, Microporous Cloth that Selectively Captures, Releases, and Concentrates Intact Extracellular Vesicles

Author

Akbarinejad, Alireza, primary, Hisey, Colin L., additional, Brewster, Diane, additional, Ashraf, Jesna, additional, Chang, Vanessa, additional, Sabet, Saman, additional, Nursalim, Yohanes, additional, Lucarelli, Valentina, additional, Blenkiron, Cherie, additional, Chamley, Larry, additional, Barker, David, additional, Williams, David E., additional, Evans, Clive W., additional, and Travas-Sejdic, Jadranka, additional

Published
2020
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49. N-Methyl-D-Aspartate Receptor Hypofunction in Meg-01 Cells Reveals a Role for Intracellular Calcium Homeostasis in Balancing Megakaryocytic-Erythroid Differentiation

Author

Hearn, James I., additional, Green, Taryn N., additional, Chopra, Martin, additional, Nursalim, Yohanes N. S., additional, Ladvanszky, Leandro, additional, Knowlton, Nicholas, additional, Blenkiron, Cherie, additional, Poulsen, Raewyn C., additional, Singleton, Dean C., additional, Bohlander, Stefan K., additional, and Kalev-Zylinska, Maggie L., additional

Published
2020
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